AlfalfaGEDB | MsG0480019562.01

M. sativa cultivar ZhongmuNo.1 / MsG0480019562.01

KEGG: K00224
GO: GO:0016491 GO:0055114
PFAM: PF08240 PF13602

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480019562.01.T01	XP_003605677.3	96.97	330	10	0	1	330	1	330	0	651

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480019562.01.T01	Q9SV68	73.939	330	85	1	1	330	1	329	4.69E-175	491

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480019562.01.T01	A0A396I9R6	96.970	330	10	0	1	330	1	330	0.0	651

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180000114.01	MsG0480019562.01	0.827429	1.572246e-54	1.517496e-51
MsG0180000284.01	MsG0480019562.01	0.802238	6.168032e-49	3.006062e-46
MsG0180000982.01	MsG0480019562.01	0.818087	2.354177e-52	1.745081e-49
MsG0180003725.01	MsG0480019562.01	0.803866	2.841259e-49	1.443658e-46
MsG0180004396.01	MsG0480019562.01	0.811330	7.390893e-51	4.561632e-48
MsG0180004599.01	MsG0480019562.01	0.829680	4.495290e-55	4.633812e-52
MsG0180005787.01	MsG0480019562.01	0.807118	5.908953e-50	3.263795e-47
MsG0180005990.01	MsG0480019562.01	0.802726	4.893393e-49	2.414776e-46
MsG0180006243.01	MsG0480019562.01	0.800908	1.155959e-48	5.447221e-46
MsG0280010595.01	MsG0480019562.01	0.802746	4.845883e-49	2.392523e-46
MsG0380011531.01	MsG0480019562.01	0.806191	9.273236e-50	5.001794e-47
MsG0380011537.01	MsG0480019562.01	0.825208	5.314573e-54	4.812620e-51
MsG0380011669.01	MsG0480019562.01	0.815520	8.864093e-52	6.125383e-49
MsG0380011895.01	MsG0480019562.01	0.825718	4.024189e-54	3.697335e-51
MsG0380014727.01	MsG0480019562.01	0.811052	8.487645e-51	5.200873e-48
MsG0480018473.01	MsG0480019562.01	0.821395	4.131419e-53	3.357150e-50
MsG0480018847.01	MsG0480019562.01	0.821128	4.760283e-53	3.839603e-50
MsG0480018995.01	MsG0480019562.01	0.873436	1.472433e-67	6.390127e-64
MsG0480019434.01	MsG0480019562.01	0.824550	7.595838e-54	6.750170e-51
MsG0480019442.01	MsG0480019562.01	0.808930	2.431150e-50	1.408072e-47
MsG0480019562.01	MsG0480020322.01	0.811373	7.233391e-51	4.469807e-48
MsG0480019562.01	MsG0480020323.01	0.816325	5.862402e-52	4.141382e-49
MsG0480019562.01	MsG0480021280.01	0.805843	1.097811e-49	5.868839e-47
MsG0480019562.01	MsG0480021917.01	0.805658	1.200150e-49	6.385550e-47
MsG0480019562.01	MsG0480022207.01	0.816470	5.439687e-52	3.857708e-49
MsG0480019562.01	MsG0480022827.01	0.811682	6.194683e-51	3.859142e-48
MsG0480019562.01	MsG0480022933.01	0.800804	1.213475e-48	5.702882e-46
MsG0480019562.01	MsG0480023025.01	0.806575	7.695782e-50	4.191627e-47
MsG0480019562.01	MsG0580024725.01	0.817184	3.762382e-52	2.720885e-49
MsG0480019562.01	MsG0580024775.01	0.845086	5.058087e-59	8.334792e-56
MsG0480019562.01	MsG0580024788.01	0.801980	6.968442e-49	3.373733e-46
MsG0480019562.01	MsG0580024935.01	0.832991	6.891329e-56	7.831320e-53
MsG0480019562.01	MsG0580026032.01	0.816165	6.365988e-52	4.477246e-49
MsG0480019562.01	MsG0580026304.01	0.827654	1.388234e-54	1.348554e-51
MsG0480019562.01	MsG0580028289.01	0.807301	5.404295e-50	2.999504e-47
MsG0480019562.01	MsG0580028991.01	0.825077	5.704434e-54	5.146725e-51
MsG0480019562.01	MsG0580029038.01	0.807787	4.262298e-50	2.395666e-47
MsG0480019562.01	MsG0580029717.01	0.802222	6.215814e-49	3.028029e-46
MsG0480019562.01	MsG0580029879.01	0.813668	2.277677e-51	1.496748e-48
MsG0480019562.01	MsG0680030415.01	0.807608	4.651964e-50	2.602430e-47
MsG0480019562.01	MsG0680030636.01	0.801449	8.956792e-49	4.278634e-46
MsG0480019562.01	MsG0680031334.01	0.867383	1.425250e-65	4.967083e-62
MsG0480019562.01	MsG0680032058.01	0.839971	1.151449e-57	1.616693e-54
MsG0480019562.01	MsG0680033459.01	0.803616	3.201278e-49	1.616106e-46
MsG0480019562.01	MsG0680035765.01	0.801221	9.974249e-49	4.737298e-46
MsG0480019562.01	MsG0780037082.01	0.807346	5.287701e-50	2.938093e-47
MsG0480019562.01	MsG0780039439.01	0.806600	7.602148e-50	4.143297e-47
MsG0480019562.01	MsG0780039628.01	0.810896	9.175138e-51	5.599021e-48
MsG0480019562.01	MsG0780040566.01	-0.808109	3.639565e-50	2.063023e-47
MsG0480019562.01	MsG0780040576.01	-0.804531	2.065816e-49	1.067648e-46
MsG0480019562.01	MsG0780041030.01	0.815326	9.792726e-52	6.730723e-49
MsG0480019562.01	MsG0780041187.01	0.803932	2.753609e-49	1.401515e-46
MsG0480019562.01	MsG0880042031.01	0.817059	4.013669e-52	2.892835e-49
MsG0480019562.01	MsG0880042055.01	0.822812	1.939084e-53	1.640378e-50
MsG0480019562.01	MsG0880042743.01	0.805711	1.169860e-49	6.233108e-47
MsG0480019562.01	MsG0880042857.01	0.883791	3.326140e-71	2.139284e-67
MsG0480019562.01	MsG0880043749.01	0.825572	4.358280e-54	3.987547e-51
MsG0480019562.01	MsG0880043867.01	0.841420	4.803542e-58	7.054719e-55
MsG0480019562.01	MsG0880044573.01	0.829987	3.783302e-55	3.934551e-52
MsG0480019562.01	MsG0880045387.01	0.830083	3.585917e-55	3.739160e-52
MsG0480019562.01	MsG0880046377.01	0.819972	8.769421e-53	6.850041e-50
MsG0480019562.01	MsG0880046901.01	0.800731	1.255974e-48	5.891761e-46
MsG0480019562.01	MsG0880046930.01	0.802559	5.297592e-49	2.602927e-46
MsG0480019562.01	MsG0880047045.01	0.804814	1.803113e-49	9.388139e-47
MsG0480019562.01	MsG0880047046.01	0.839139	1.894267e-57	2.591899e-54
MsG0480019562.01	MsG0880047267.01	0.808066	3.716783e-50	2.104441e-47
MsG0480019562.01	MsG0880047594.01	0.846724	1.814420e-59	3.152181e-56
MsG0380016055.01	MsG0480019562.01	0.814560	1.447735e-51	9.746470e-49
MsG0380016182.01	MsG0480019562.01	0.849771	2.611243e-60	5.004073e-57
MsG0380016187.01	MsG0480019562.01	0.806779	6.968949e-50	3.815703e-47
MsG0380016391.01	MsG0480019562.01	0.819725	9.987535e-53	7.747615e-50
MsG0380017023.01	MsG0480019562.01	0.826682	2.372587e-54	2.240551e-51
MsG0380017048.01	MsG0480019562.01	0.803045	4.204423e-49	2.091528e-46
MsG0380017085.01	MsG0480019562.01	0.844589	6.883493e-59	1.116596e-55
MsG0480018207.01	MsG0480019562.01	-0.826418	2.742906e-54	2.570946e-51
MsG0480018214.01	MsG0480019562.01	0.824952	6.107520e-54	5.490576e-51
MsG0480018236.01	MsG0480019562.01	0.817262	3.611984e-52	2.617687e-49
MsG0480018318.01	MsG0480019562.01	0.800460	1.426104e-48	6.643932e-46
MsG0480018402.01	MsG0480019562.01	0.808804	2.586423e-50	1.493022e-47
MsG0280007023.01	MsG0480019562.01	0.804876	1.750367e-49	9.128415e-47
MsG0280007496.01	MsG0480019562.01	0.804549	2.048397e-49	1.059141e-46
MsG0280008113.01	MsG0480019562.01	0.812474	4.162537e-51	2.649290e-48
MsG0280008738.01	MsG0480019562.01	0.802993	4.309055e-49	2.140730e-46
MsG0280008936.01	MsG0480019562.01	0.806551	7.785957e-50	4.238334e-47
MsG0280009637.01	MsG0480019562.01	-0.808825	2.559923e-50	1.478568e-47
MsG0280009739.01	MsG0480019562.01	0.804693	1.910699e-49	9.916882e-47
MsG0280010288.01	MsG0480019562.01	0.815760	7.836101e-52	5.450855e-49

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480019562.01.T01	MTR_4g036310	82.371	329	57	1	1	329	1	328	0.0	555
MsG0480019562.01.T01	MTR_4g033205	80.606	330	63	1	1	330	1	329	0.0	541
MsG0480019562.01.T01	MTR_4g032995	80.606	330	63	1	1	330	1	329	0.0	540
MsG0480019562.01.T01	MTR_4g036260	97.048	271	8	0	60	330	38	308	0.0	536
MsG0480019562.01.T01	MTR_4g036170	74.164	329	59	4	1	329	1	303	9.49e-169	471
MsG0480019562.01.T01	MTR_7g055743	67.314	309	100	1	21	329	61	368	4.16e-149	424
MsG0480019562.01.T01	MTR_8g064610	35.673	342	168	11	6	330	76	382	3.87e-43	153
MsG0480019562.01.T01	MTR_4g036150	80.282	71	14	0	1	71	1	71	2.32e-38	131
MsG0480019562.01.T01	MTR_8g035630	32.738	336	185	10	4	328	2	307	7.14e-34	127
MsG0480019562.01.T01	MTR_8g035880	32.738	336	185	10	4	328	2	307	1.61e-33	126
MsG0480019562.01.T01	MTR_6g012170	33.134	335	185	8	7	330	12	318	8.41e-33	124
MsG0480019562.01.T01	MTR_7g034395	34.203	345	182	13	1	330	1	315	4.11e-31	119
MsG0480019562.01.T01	MTR_2g078520	29.446	343	212	9	7	329	27	359	1.58e-27	110
MsG0480019562.01.T01	MTR_6g012160	31.613	310	175	7	7	307	12	293	5.24e-26	105
MsG0480019562.01.T01	MTR_4g050760	27.439	328	209	10	16	328	10	323	2.07e-21	93.2
MsG0480019562.01.T01	MTR_2g012380	27.439	328	209	10	16	328	10	323	2.07e-21	93.2
MsG0480019562.01.T01	MTR_2g012420	27.245	323	206	10	21	328	15	323	1.16e-19	88.6
MsG0480019562.01.T01	MTR_2g012380	30.876	217	136	6	16	229	10	215	4.13e-19	85.9
MsG0480019562.01.T01	MTR_4g086410	30.699	329	190	13	24	328	306	620	3.49e-17	82.8
MsG0480019562.01.T01	MTR_4g086400	30.793	328	191	13	24	328	166	480	3.55e-17	82.4
MsG0480019562.01.T01	MTR_4g086400	30.793	328	191	13	24	328	306	620	5.64e-17	82.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480019562.01.T01	AT4G13010	73.939	330	85	1	1	330	1	329	4.78e-176	491
MsG0480019562.01.T01	AT1G23740	35.294	340	176	11	4	330	76	384	1.44e-41	149
MsG0480019562.01.T01	AT3G15090	30.650	323	196	6	26	329	52	365	3.77e-29	115
MsG0480019562.01.T01	AT1G49670	31.737	334	186	16	19	328	301	616	2.30e-18	86.3
MsG0480019562.01.T01	AT1G49670	30.398	352	190	17	19	328	301	639	1.74e-15	77.8
MsG0480019562.01.T01	AT4G21580	27.329	322	209	13	21	329	15	324	1.79e-15	76.6
MsG0480019562.01.T01	AT4G21580	27.414	321	204	13	21	329	15	318	6.14e-15	74.7
MsG0480019562.01.T01	AT5G61510	25.767	326	219	8	16	330	93	406	1.50e-12	68.6
MsG0480019562.01.T01	AT5G61510	25.545	321	216	8	21	330	41	349	2.74e-12	67.4

Find 80 sgRNAs with CRISPR-Local

Find 323 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CGTGACGCCCTCACAGAAAT+TGG	0.273115	4:-22267052	MsG0480019562.01.T01:CDS
AGTAAAACTTCATTGGTCTT+TGG	0.287529	4:+22271154	None:intergenic
AAAGAAGAATCCGAAGATTC+TGG	0.304846	4:-22271389	MsG0480019562.01.T01:five_prime_UTR
GACTGAATCTTCCAATCAAC+TGG	0.313131	4:+22271106	None:intergenic
CACGTGGTTGTTCCCTAGTT+TGG	0.331494	4:+22266940	None:intergenic
AGATTCAGTCTGGTGCTCTA+AGG	0.338199	4:-22271093	MsG0480019562.01.T01:CDS
TCCTATGGCGGAGGAGTCTC+TGG	0.355413	4:-22271318	MsG0480019562.01.T01:CDS
CAATTTGAGTAAAACTTCAT+TGG	0.361551	4:+22271147	None:intergenic
AACTGTTAAGCGTGAGGGCT+TGG	0.380438	4:-22266636	MsG0480019562.01.T01:CDS
TGATCCTAATTTGAGTGAAA+AGG	0.380673	4:-22266756	MsG0480019562.01.T01:CDS
GATCCTAATTTGAGTGAAAA+GGG	0.382966	4:-22266755	MsG0480019562.01.T01:CDS
CCTTCAGCAGCTGAGACTTC+AGG	0.386418	4:+22267106	None:intergenic
TGGCGAGCAGAAGAATGTTT+TGG	0.398444	4:-22267008	MsG0480019562.01.T01:CDS
ATTGGAGGAATTAAGCTTGA+TGG	0.403108	4:-22267034	MsG0480019562.01.T01:CDS
AGCTGAGACTTCAGGTGGTC+TGG	0.404146	4:+22267114	None:intergenic
AGTATTGATGGCCATGCTAC+TGG	0.420478	4:-22266521	MsG0480019562.01.T01:CDS
GCTGTTCAACTTGCCAAACT+AGG	0.421441	4:-22266953	MsG0480019562.01.T01:CDS
AGAGATTGGACCACAAGTTA+AGG	0.426820	4:-22269945	MsG0480019562.01.T01:CDS
TGAGCAAAGCTGAAGATGCT+TGG	0.430166	4:-22266550	MsG0480019562.01.T01:CDS
GGTGCGAGTTTACCTATTGC+CGG	0.430821	4:-22267085	MsG0480019562.01.T01:CDS
CTCTTCAGTGATGTTCCCTC+TGG	0.430862	4:+22266842	None:intergenic
ATCGACTTTGTTAAGAGCTT+AGG	0.447363	4:-22266893	MsG0480019562.01.T01:CDS
TTGGTCTTTGGAGTTGGTAC+AGG	0.448329	4:+22271166	None:intergenic
GAGCAAAGCTGAAGATGCTT+GGG	0.448420	4:-22266549	MsG0480019562.01.T01:CDS
CGGAGGAGTCTCTGGATTGA+AGG	0.450051	4:-22271310	MsG0480019562.01.T01:intron
GCAAAAGTCAACAATGAACT+TGG	0.454364	4:+22266707	None:intergenic
GCTTGGGCTAAGAGTATTGA+TGG	0.455624	4:-22266533	MsG0480019562.01.T01:CDS
CTAGCATCAACCCAGTTGAT+TGG	0.458227	4:-22271117	MsG0480019562.01.T01:CDS
CGAGCTGTGAGACCGGCAAT+AGG	0.465576	4:+22267073	None:intergenic
GCAGGAGAGGTAGTAGAGAT+TGG	0.465867	4:-22269959	MsG0480019562.01.T01:CDS
GGAATTAAGCTTGATGGGAC+TGG	0.474637	4:-22267028	MsG0480019562.01.T01:CDS
GCATCTTCAGCTTTGCTCAA+AGG	0.476004	4:+22266554	None:intergenic
ACTTCATTGGTCTTTGGAGT+TGG	0.486575	4:+22271160	None:intergenic
GTCAACAATGAACTTGGGCC+AGG	0.489320	4:+22266713	None:intergenic
TTGGAGGAATTAAGCTTGAT+GGG	0.490626	4:-22267033	MsG0480019562.01.T01:CDS
ACTGCTGCTTCAGGTGGTGT+AGG	0.492958	4:-22266983	MsG0480019562.01.T01:CDS
GGCGTCACGAGCTGTGAGAC+CGG	0.493743	4:+22267066	None:intergenic
GTTGTCGTGGATTTAACACC+TGG	0.495781	4:-22266731	MsG0480019562.01.T01:CDS
ACATCACTGAAGAGTCCATC+TGG	0.503456	4:-22266833	MsG0480019562.01.T01:CDS
CTGTTCAACTTGCCAAACTA+GGG	0.504458	4:-22266952	MsG0480019562.01.T01:CDS
GCAGTAATACATTGCACCAC+TGG	0.505627	4:-22266797	MsG0480019562.01.T01:CDS
CGCTTAACAGTTACAACGAA+TGG	0.512764	4:+22266647	None:intergenic
GCTCTTCAGTACAGTTCCTA+TGG	0.513825	4:-22271333	MsG0480019562.01.T01:CDS
AATTCAGGCACTGATGTAGC+AGG	0.527374	4:-22269977	MsG0480019562.01.T01:intron
AATTCCTCCAATTTCTGTGA+GGG	0.528728	4:+22267045	None:intergenic
GTTGATTGGAAGATTCAGTC+TGG	0.533264	4:-22271103	MsG0480019562.01.T01:CDS
GTCGATGTTACGAGCACCAC+AGG	0.534676	4:+22266910	None:intergenic
GCTTTAAAATCCTTAACTTG+TGG	0.541477	4:+22269935	None:intergenic
GATTCAGTCTGGTGCTCTAA+GGG	0.542295	4:-22271092	MsG0480019562.01.T01:CDS
TTGGTAACTGCTGCTTCAGG+TGG	0.558002	4:-22266989	MsG0480019562.01.T01:CDS
CTCGCTCAACTAGTGAAGGA+TGG	0.564162	4:-22266608	MsG0480019562.01.T01:CDS
TAAGAGCTTAGGTGCTGATG+AGG	0.567525	4:-22266882	MsG0480019562.01.T01:CDS
GTTGTAACTGTTAAGCGTGA+GGG	0.570258	4:-22266641	MsG0480019562.01.T01:CDS
TCCAGAGACTCCTCCGCCAT+AGG	0.573666	4:+22271317	None:intergenic
CAGTACAGTTCCTATGGCGG+AGG	0.575427	4:-22271327	MsG0480019562.01.T01:CDS
CCTGAAGTCTCAGCTGCTGA+AGG	0.576984	4:-22267106	MsG0480019562.01.T01:CDS
CGTTGTAACTGTTAAGCGTG+AGG	0.578759	4:-22266642	MsG0480019562.01.T01:CDS
TCCTAATTTGAGTGAAAAGG+GGG	0.582411	4:-22266753	MsG0480019562.01.T01:CDS
CACCACAGGTGGCTGTCACG+TGG	0.590671	4:+22266924	None:intergenic
ACATCTCGCTCAACTAGTGA+AGG	0.592170	4:-22266612	MsG0480019562.01.T01:CDS
AGGCACTGATGTAGCAGGAG+AGG	0.593122	4:-22269972	MsG0480019562.01.T01:CDS
TGTGAATGACAGTATGGAGG+TGG	0.601090	4:-22267178	MsG0480019562.01.T01:intron
ATCCTAATTTGAGTGAAAAG+GGG	0.606192	4:-22266754	MsG0480019562.01.T01:CDS
CAAAAGTCAACAATGAACTT+GGG	0.607885	4:+22266708	None:intergenic
GACGCCCTCACAGAAATTGG+AGG	0.616536	4:-22267049	MsG0480019562.01.T01:CDS
TGTGTCATACAAGGTATGTG+AGG	0.642683	4:+22271046	None:intergenic
TGTTAAGCTCTCGCTAGCCA+CGG	0.646494	4:+22267141	None:intergenic
TGGACTAGCTGAGTTTGCCG+TGG	0.647341	4:-22267158	MsG0480019562.01.T01:CDS
TAATTCCTCCAATTTCTGTG+AGG	0.652507	4:+22267044	None:intergenic
CTTGACTACAAAACTCCAGA+GGG	0.654718	4:-22266857	MsG0480019562.01.T01:CDS
TCAGCAGCTGAGACTTCAGG+TGG	0.657763	4:+22267109	None:intergenic
CTTCAGTACAGTTCCTATGG+CGG	0.668012	4:-22271330	MsG0480019562.01.T01:CDS
TTATGTGAATGACAGTATGG+AGG	0.672015	4:-22267181	MsG0480019562.01.T01:intron
ACTGAATCTTCCAATCAACT+GGG	0.677961	4:+22271107	None:intergenic
AACCACGTGACAGCCACCTG+TGG	0.678724	4:-22266926	MsG0480019562.01.T01:CDS
TTAGGATCAAAAGTTGACCA+TGG	0.679639	4:+22266770	None:intergenic
AGTTGACCATGGTATTCCAG+TGG	0.688020	4:+22266781	None:intergenic
TCTTGACTACAAAACTCCAG+AGG	0.691725	4:-22266858	MsG0480019562.01.T01:CDS
ATTGCACCACTGGAATACCA+TGG	0.709876	4:-22266787	MsG0480019562.01.T01:CDS
GATGTTACGAGCACCACAGG+TGG	0.713051	4:+22266913	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AATCAAAGAAAAAAAAATAT+AGG	+	Chr4:22267756-22267775	None:intergenic	10.0%
!!	ATAGAAATTAAAATATTGTT+TGG	+	Chr4:22269445-22269464	None:intergenic	10.0%
!!	TATGAAATAAAAAAAAAAGT+TGG	+	Chr4:22270649-22270668	None:intergenic	10.0%
!!!	GTGATTTTTTTTTTTTTTTT+TGG	+	Chr4:22269876-22269895	None:intergenic	10.0%
!!	AATAAAAAAAAAAGTTGGAT+TGG	+	Chr4:22270644-22270663	None:intergenic	15.0%
!!	AATTTGAAAAAGAACAATAA+AGG	-	Chr4:22267592-22267611	MsG0480019562.01.T01:intron	15.0%
!!	CATTTATATATTAATTACAC+TGG	-	Chr4:22270671-22270690	MsG0480019562.01.T01:intron	15.0%
!!	TTTAAAAACTCATTTATTTC+TGG	+	Chr4:22269759-22269778	None:intergenic	15.0%
!!!	AATTATAAATATAGGTTGAA+AGG	+	Chr4:22268871-22268890	None:intergenic	15.0%
!!!	ATTATAAATATAGGTTGAAA+GGG	+	Chr4:22268870-22268889	None:intergenic	15.0%
!!	AACCAAAATAAATTCTTCTT+TGG	-	Chr4:22269356-22269375	MsG0480019562.01.T01:intron	20.0%
!!	AATTTACACTTGAATTAACA+CGG	+	Chr4:22268081-22268100	None:intergenic	20.0%
!!	ACCAAAATAAATTCTTCTTT+GGG	-	Chr4:22269357-22269376	MsG0480019562.01.T01:intron	20.0%
!!	ATCTGATTCAATTCAATTAT+TGG	-	Chr4:22266687-22266706	MsG0480019562.01.T01:CDS	20.0%
!!	ATTTCTACAAGTGAAATTAT+AGG	+	Chr4:22266908-22266927	None:intergenic	20.0%
!!	CTAGAAAACTGAAATATTAT+TGG	-	Chr4:22267163-22267182	MsG0480019562.01.T01:CDS	20.0%
!!	GTAGTCTGAATTATAAATAT+AGG	+	Chr4:22268879-22268898	None:intergenic	20.0%
!!	TAAATTACTAATCTATAGCT+TGG	-	Chr4:22268501-22268520	MsG0480019562.01.T01:intron	20.0%
!!	TTAATAAATCATACCTTTCT+AGG	+	Chr4:22270248-22270267	None:intergenic	20.0%
!!	TTGCAATATCAATAAAATGA+GGG	+	Chr4:22268248-22268267	None:intergenic	20.0%
!!	TTTCATATCTCCATAATTTA+GGG	+	Chr4:22269711-22269730	None:intergenic	20.0%
!!	TTTGCAATATCAATAAAATG+AGG	+	Chr4:22268249-22268268	None:intergenic	20.0%
!!!	AATTTAGTTTCTTTACATAC+TGG	+	Chr4:22268487-22268506	None:intergenic	20.0%
!!!	ACTCATTTTTGCTTATAAAT+AGG	+	Chr4:22269150-22269169	None:intergenic	20.0%
!!!	ATCAATATTTTGATATGTGA+TGG	+	Chr4:22268277-22268296	None:intergenic	20.0%
!!!	GAATTTTAAACTCAAACAAA+TGG	+	Chr4:22268762-22268781	None:intergenic	20.0%
!!!	GGATTTTAGTTTATAAAAAC+AGG	-	Chr4:22267653-22267672	MsG0480019562.01.T01:intron	20.0%
!!!	TATAGATAGATTACTACTTT+TGG	-	Chr4:22270016-22270035	MsG0480019562.01.T01:intron	20.0%
!!!	TTTTAAGAATTAGATGGAAA+TGG	-	Chr4:22270189-22270208	MsG0480019562.01.T01:intron	20.0%
!!!	TTTTCATATCTCCATAATTT+AGG	+	Chr4:22269712-22269731	None:intergenic	20.0%
!!!	TTTTTGCTTATAAATAGGAA+TGG	+	Chr4:22269145-22269164	None:intergenic	20.0%
!	AAATTGAAATGCAATCATCT+TGG	-	Chr4:22269403-22269422	MsG0480019562.01.T01:intron	25.0%
!	AAGATGATTGCATTTCAATT+TGG	+	Chr4:22269404-22269423	None:intergenic	25.0%
!	AATAAAGTTTACTACCATGA+TGG	-	Chr4:22268606-22268625	MsG0480019562.01.T01:intron	25.0%
!	AATAACCTTCTCTTTAGTAA+AGG	-	Chr4:22270434-22270453	MsG0480019562.01.T01:intron	25.0%
!	AATTAACAGCATAAATCATG+TGG	+	Chr4:22270509-22270528	None:intergenic	25.0%
!	AATTAAGATTCCTTTGATCT+TGG	-	Chr4:22267893-22267912	MsG0480019562.01.T01:intron	25.0%
!	ACTAAAAATAACTCAGAGAT+AGG	+	Chr4:22267277-22267296	None:intergenic	25.0%
!	AGAAAAAGTAAAAAAGAAGG+TGG	+	Chr4:22266884-22266903	None:intergenic	25.0%
!	ATCACTAGAAATAGAATCTA+GGG	-	Chr4:22269312-22269331	MsG0480019562.01.T01:intron	25.0%
!	ATTAAGATTCCTTTGATCTT+GGG	-	Chr4:22267894-22267913	MsG0480019562.01.T01:intron	25.0%
!	CAATTTGAGTAAAACTTCAT+TGG	+	Chr4:22266755-22266774	None:intergenic	25.0%
!	CACTTAACACATGAAATTAA+CGG	-	Chr4:22269896-22269915	MsG0480019562.01.T01:intron	25.0%
!	CACTTATCAACAAATCTAAA+AGG	-	Chr4:22269528-22269547	MsG0480019562.01.T01:intron	25.0%
!	CTAAAAATAACTCAGAGATA+GGG	+	Chr4:22267276-22267295	None:intergenic	25.0%
!	GAAATATTATTGGATGTTTC+TGG	-	Chr4:22267173-22267192	MsG0480019562.01.T01:intron	25.0%
!	GATGTATTTGGTTAAAAATG+TGG	+	Chr4:22269196-22269215	None:intergenic	25.0%
!	GCAGTTTATAATTAGTAATG+TGG	+	Chr4:22269090-22269109	None:intergenic	25.0%
!	GGAGATATGAAAATTTCTTA+AGG	-	Chr4:22269719-22269738	MsG0480019562.01.T01:intron	25.0%
!	TAAAAATAACTCAGAGATAG+GGG	+	Chr4:22267275-22267294	None:intergenic	25.0%
!	TATCACTAGAAATAGAATCT+AGG	-	Chr4:22269311-22269330	MsG0480019562.01.T01:intron	25.0%
!	TCATTCACATAACACAAAAT+TGG	+	Chr4:22270710-22270729	None:intergenic	25.0%
!	TTATCTATAAGTACCCAATT+TGG	+	Chr4:22269989-22270008	None:intergenic	25.0%
!!	ACCCAAAGAAGAATTTATTT+TGG	+	Chr4:22269361-22269380	None:intergenic	25.0%
!!	ATTCTGGAAAAAGTTAGATA+TGG	-	Chr4:22266526-22266545	MsG0480019562.01.T01:CDS	25.0%
!!	CAATGAAGTTTTACTCAAAT+TGG	-	Chr4:22266753-22266772	MsG0480019562.01.T01:CDS	25.0%
!!	TGTTTCTCCAACAAATTTTT+GGG	-	Chr4:22269504-22269523	MsG0480019562.01.T01:intron	25.0%
!!	TTGTTGATTGATTTACCAAT+TGG	+	Chr4:22270042-22270061	None:intergenic	25.0%
!!	TTTAAAATTCCTTTAGCAAG+AGG	-	Chr4:22268772-22268791	MsG0480019562.01.T01:intron	25.0%
!!!	AATTCTAGCTTGTATCAATT+TGG	-	Chr4:22269784-22269803	MsG0480019562.01.T01:intron	25.0%
!!!	ACTCGTTTTTTCTTATAGAT+AGG	-	Chr4:22269227-22269246	MsG0480019562.01.T01:intron	25.0%
!!!	CTAAGGTTTTAAGAATTAGA+TGG	-	Chr4:22270183-22270202	MsG0480019562.01.T01:intron	25.0%
!!!	CTTTTAGATTTGTTGATAAG+TGG	+	Chr4:22269530-22269549	None:intergenic	25.0%
!!!	GACAATATTTTGATAAGTCA+GGG	-	Chr4:22269931-22269950	MsG0480019562.01.T01:CDS	25.0%
!!!	TCAAAGAAAGTTGATGTATT+TGG	+	Chr4:22269208-22269227	None:intergenic	25.0%
!!!	TGACAATATTTTGATAAGTC+AGG	-	Chr4:22269930-22269949	MsG0480019562.01.T01:CDS	25.0%
!!!	TTTTGAAAGAGATGAGAAAT+GGG	+	Chr4:22266631-22266650	None:intergenic	25.0%
!!!	TTTTTCAAAACCGAATTGAT+CGG	-	Chr4:22267238-22267257	MsG0480019562.01.T01:intron	25.0%
	AAAAATCATTGTTGAGCCAT+AGG	-	Chr4:22271401-22271420	MsG0480019562.01.T01:five_prime_UTR	30.0%
	AAAAGATAAACTCAAAACCG+TGG	-	Chr4:22268692-22268711	MsG0480019562.01.T01:intron	30.0%
	AAATCATACCTTTCTAGGTA+AGG	+	Chr4:22270243-22270262	None:intergenic	30.0%
	AAGCAAAAATGAGTCAAAGA+AGG	-	Chr4:22269156-22269175	MsG0480019562.01.T01:intron	30.0%
	AATGGTAAAACCATGGTAAA+TGG	+	Chr4:22268744-22268763	None:intergenic	30.0%
	ACAATAAAGGAATAGCCTAA+AGG	-	Chr4:22267605-22267624	MsG0480019562.01.T01:intron	30.0%
	AGACAACTGGAAATAGATAT+TGG	+	Chr4:22268441-22268460	None:intergenic	30.0%
	ATACACCTTTACTAAAGAGA+AGG	+	Chr4:22270442-22270461	None:intergenic	30.0%
	ATCCTAATTTGAGTGAAAAG+GGG	-	Chr4:22271145-22271164	MsG0480019562.01.T01:CDS	30.0%
	ATGTCATAAGCTATTCCTTT+AGG	+	Chr4:22267623-22267642	None:intergenic	30.0%
	ATGTTAGACAGACAACAAAT+TGG	+	Chr4:22270113-22270132	None:intergenic	30.0%
	ATTACCATGTGATTAAGAGA+AGG	+	Chr4:22270359-22270378	None:intergenic	30.0%
	CAAAAGTCAACAATGAACTT+GGG	+	Chr4:22271194-22271213	None:intergenic	30.0%
	CAAATACTGTAATACTACGA+TGG	+	Chr4:22268550-22268569	None:intergenic	30.0%
	CATGGTAGTAAACTTTATTC+TGG	+	Chr4:22268605-22268624	None:intergenic	30.0%
	CGTTAATTTCATGTGTTAAG+TGG	+	Chr4:22269898-22269917	None:intergenic	30.0%
	CTAAACTAGTACATCCAAAT+TGG	+	Chr4:22267090-22267109	None:intergenic	30.0%
	GAAACAACTTAAGATATGTG+TGG	-	Chr4:22266988-22267007	MsG0480019562.01.T01:CDS	30.0%
	GATCCTAATTTGAGTGAAAA+GGG	-	Chr4:22271144-22271163	MsG0480019562.01.T01:CDS	30.0%
	GATTAAATCGATAAAACGCT+AGG	-	Chr4:22268398-22268417	MsG0480019562.01.T01:intron	30.0%
	GGCAGAAAAAGTAAAAAAGA+AGG	+	Chr4:22266887-22266906	None:intergenic	30.0%
	TAACACATGAAATTAACGGA+GGG	-	Chr4:22269900-22269919	MsG0480019562.01.T01:intron	30.0%
	TAATAACACATACCCTTTCA+CGG	-	Chr4:22268153-22268172	MsG0480019562.01.T01:intron	30.0%
	TAATGGAAAACAGTAACCTA+AGG	+	Chr4:22268047-22268066	None:intergenic	30.0%
	TACTTTAATCCTCTTGCTAA+AGG	+	Chr4:22268784-22268803	None:intergenic	30.0%
	TAGTACACGTAAGAAAAAGT+CGG	+	Chr4:22269813-22269832	None:intergenic	30.0%
	TATTGCAAGTAAAAGTGAGT+AGG	+	Chr4:22267872-22267891	None:intergenic	30.0%
	TCAAACAAATGGTAAAACCA+TGG	+	Chr4:22268751-22268770	None:intergenic	30.0%
	TGATCCTAATTTGAGTGAAA+AGG	-	Chr4:22271143-22271162	MsG0480019562.01.T01:CDS	30.0%
	TTAACACATGAAATTAACGG+AGG	-	Chr4:22269899-22269918	MsG0480019562.01.T01:intron	30.0%
	TTCAACAGGTATAATAACCA+CGG	+	Chr4:22268712-22268731	None:intergenic	30.0%
	TTCTTATAGATAGGAACAGA+GGG	-	Chr4:22269236-22269255	MsG0480019562.01.T01:intron	30.0%
	TTTCTTATAGATAGGAACAG+AGG	-	Chr4:22269235-22269254	MsG0480019562.01.T01:intron	30.0%
!	AAAAAAGTTGGATTGGAGAT+AGG	+	Chr4:22270637-22270656	None:intergenic	30.0%
!	AATATAGGTTGAAAGGGAAA+AGG	+	Chr4:22268864-22268883	None:intergenic	30.0%
!	ATATAGGTTGAAAGGGAAAA+GGG	+	Chr4:22268863-22268882	None:intergenic	30.0%
!	GTGTTTCTCCAACAAATTTT+TGG	-	Chr4:22269503-22269522	MsG0480019562.01.T01:intron	30.0%
!	TGTACTAGTTTAGTTTCTTC+AGG	-	Chr4:22267097-22267116	MsG0480019562.01.T01:CDS	30.0%
!!	AAAATTTGTTGGAGAAACAC+AGG	+	Chr4:22269503-22269522	None:intergenic	30.0%
!!	AGTAAAACTTCATTGGTCTT+TGG	+	Chr4:22266748-22266767	None:intergenic	30.0%
!!	CTAACTTTTTCCAGAATCTT+CGG	+	Chr4:22266523-22266542	None:intergenic	30.0%
!!	CTTTTGAAAGAGATGAGAAA+TGG	+	Chr4:22266632-22266651	None:intergenic	30.0%
!!	GCTTTAAAATCCTTAACTTG+TGG	+	Chr4:22267967-22267986	None:intergenic	30.0%
!!	GTGCTTAAGCTTTAAAAACT+AGG	+	Chr4:22269479-22269498	None:intergenic	30.0%
!!!	CAAGTTAAGGATTTTAAAGC+TGG	-	Chr4:22267967-22267986	MsG0480019562.01.T01:intron	30.0%
!!!	TACTTTTTCAAAGAAGCGAT+TGG	-	Chr4:22271227-22271246	MsG0480019562.01.T01:intron	30.0%
	AAAAGACAAAGACTTGACTC+AGG	-	Chr4:22269617-22269636	MsG0480019562.01.T01:intron	35.0%
	AAAGAAGAATCCGAAGATTC+TGG	-	Chr4:22266510-22266529	MsG0480019562.01.T01:CDS	35.0%
	AATCAATCAACAACATGCAC+TGG	-	Chr4:22270049-22270068	MsG0480019562.01.T01:intron	35.0%
	AATGGAGTATATCCATACCA+TGG	+	Chr4:22269127-22269146	None:intergenic	35.0%
	AATTATTAGTCACAGCTCAC+AGG	+	Chr4:22268639-22268658	None:intergenic	35.0%
	AATTCAAGTCCCAAGATCAA+AGG	+	Chr4:22267906-22267925	None:intergenic	35.0%
	AATTCCTCCAATTTCTGTGA+GGG	+	Chr4:22270857-22270876	None:intergenic	35.0%
	ACAAACTACATCTCCAAACA+AGG	+	Chr4:22270279-22270298	None:intergenic	35.0%
	ACTGAATCTTCCAATCAACT+GGG	+	Chr4:22266795-22266814	None:intergenic	35.0%
	AGACAACAAATTGGAATGGT+TGG	+	Chr4:22270104-22270123	None:intergenic	35.0%
	AGACAGACAACAAATTGGAA+TGG	+	Chr4:22270108-22270127	None:intergenic	35.0%
	AGGTAAGGCTAATCAAAACA+AGG	+	Chr4:22270228-22270247	None:intergenic	35.0%
	AGTACACGTAAGAAAAAGTC+GGG	+	Chr4:22269812-22269831	None:intergenic	35.0%
	ATACTACGATGGTACATTTC+TGG	+	Chr4:22268539-22268558	None:intergenic	35.0%
	ATATCTCGTGATCAATCATG+TGG	+	Chr4:22270311-22270330	None:intergenic	35.0%
	ATTGGAGGAATTAAGCTTGA+TGG	-	Chr4:22270865-22270884	MsG0480019562.01.T01:intron	35.0%
	CAAGTGATTATGCTCATCAT+CGG	+	Chr4:22266944-22266963	None:intergenic	35.0%
	CAATGTCTATGCTAACAGAA+CGG	-	Chr4:22269285-22269304	MsG0480019562.01.T01:intron	35.0%
	CATGTGATTAAGAGAAGGTA+AGG	+	Chr4:22270354-22270373	None:intergenic	35.0%
	CTGGTTTCAGTGAATAAAAG+AGG	+	Chr4:22268468-22268487	None:intergenic	35.0%
	CTGTTAGCATAGACATTGTA+TGG	+	Chr4:22269283-22269302	None:intergenic	35.0%
	CTTACCTTCTCTTAATCACA+TGG	-	Chr4:22270352-22270371	MsG0480019562.01.T01:intron	35.0%
	GCAAAAGTCAACAATGAACT+TGG	+	Chr4:22271195-22271214	None:intergenic	35.0%
	GCGGTCTGAATTATAAATAC+AGG	+	Chr4:22268823-22268842	None:intergenic	35.0%
	GCTTATGACATGTCTAAGAA+TGG	-	Chr4:22267632-22267651	MsG0480019562.01.T01:intron	35.0%
	GGTTATTATACCTGTTGAAC+AGG	-	Chr4:22268713-22268732	MsG0480019562.01.T01:intron	35.0%
	GTGATGCTAAGTTTCTGTTT+GGG	-	Chr4:22267431-22267450	MsG0480019562.01.T01:intron	35.0%
	GTGTTATGTGAATGACAGTA+TGG	-	Chr4:22270715-22270734	MsG0480019562.01.T01:intron	35.0%
	TAAAAGAGGAAGAAGACAAC+TGG	+	Chr4:22268454-22268473	None:intergenic	35.0%
	TAAGTGGCCCAAAAATTTGT+TGG	+	Chr4:22269514-22269533	None:intergenic	35.0%
	TAATTCCTCCAATTTCTGTG+AGG	+	Chr4:22270858-22270877	None:intergenic	35.0%
	TAGTACACGTAAGCAAAAGT+CGG	-	Chr4:22268374-22268393	MsG0480019562.01.T01:intron	35.0%
	TAGTGAAAAACCACATGAAC+TGG	-	Chr4:22267204-22267223	MsG0480019562.01.T01:intron	35.0%
	TCCTAATTTGAGTGAAAAGG+GGG	-	Chr4:22271146-22271165	MsG0480019562.01.T01:CDS	35.0%
	TCTCTTTCTTTCACTTCCTT+AGG	-	Chr4:22268028-22268047	MsG0480019562.01.T01:intron	35.0%
	TGTCACGTTAAGTGATATTC+TGG	-	Chr4:22268340-22268359	MsG0480019562.01.T01:intron	35.0%
	TGTGATGCTAAGTTTCTGTT+TGG	-	Chr4:22267430-22267449	MsG0480019562.01.T01:intron	35.0%
	TTAAGAGAAGGTAAGGTTAG+AGG	+	Chr4:22270347-22270366	None:intergenic	35.0%
	TTAGGATCAAAAGTTGACCA+TGG	+	Chr4:22271132-22271151	None:intergenic	35.0%
	TTATGTGAATGACAGTATGG+AGG	-	Chr4:22270718-22270737	MsG0480019562.01.T01:intron	35.0%
	TTGGAGGAATTAAGCTTGAT+GGG	-	Chr4:22270866-22270885	MsG0480019562.01.T01:intron	35.0%
!	ATCGACTTTGTTAAGAGCTT+AGG	-	Chr4:22271006-22271025	MsG0480019562.01.T01:intron	35.0%
!	CAATGATTTTTCCAGTAGCA+TGG	+	Chr4:22271392-22271411	None:intergenic	35.0%
!	TCCAAATTGGCATCGAAAAA+CGG	+	Chr4:22267077-22267096	None:intergenic	35.0%
!	TCTTTTACCCACAAATGAGT+TGG	+	Chr4:22268678-22268697	None:intergenic	35.0%
!!	GATTACTACTTTTGGCCAAT+TGG	-	Chr4:22270024-22270043	MsG0480019562.01.T01:intron	35.0%
!!	TTGATTAGCCTTACCTAGAA+AGG	-	Chr4:22270232-22270251	MsG0480019562.01.T01:intron	35.0%
!!!	GCATCGTATTTTTTACCAGA+TGG	+	Chr4:22271084-22271103	None:intergenic	35.0%
	AAACCACATGAACTGGATTC+AGG	-	Chr4:22267211-22267230	MsG0480019562.01.T01:intron	40.0%
	AACATGTGGTACATATGCAC+CGG	-	Chr4:22270571-22270590	MsG0480019562.01.T01:intron	40.0%
	AACCGACTGAAACTAACAGA+AGG	+	Chr4:22268309-22268328	None:intergenic	40.0%
	AACTACATCTCCAAACAAGG+AGG	+	Chr4:22270276-22270295	None:intergenic	40.0%
	ACACGGATGAGAGGAAATAA+TGG	+	Chr4:22268064-22268083	None:intergenic	40.0%
	AGAGATTGGACCACAAGTTA+AGG	-	Chr4:22267954-22267973	MsG0480019562.01.T01:intron	40.0%
	AGTACACGTAAGCAAAAGTC+GGG	-	Chr4:22268375-22268394	MsG0480019562.01.T01:intron	40.0%
	ATGCTAAGTTTCTGTTTGGG+AGG	-	Chr4:22267434-22267453	MsG0480019562.01.T01:intron	40.0%
	ATGTCTGGTAGGGTCAAATA+AGG	+	Chr4:22270136-22270155	None:intergenic	40.0%
	ATTAACCTTGCCTCCTTGTT+TGG	-	Chr4:22270263-22270282	MsG0480019562.01.T01:intron	40.0%
	CAACTTAAGATATGTGTGGC+TGG	-	Chr4:22266992-22267011	MsG0480019562.01.T01:CDS	40.0%
	CGCTTAACAGTTACAACGAA+TGG	+	Chr4:22271255-22271274	None:intergenic	40.0%
	CTACCTACAGCAATTACAAG+CGG	-	Chr4:22268895-22268914	MsG0480019562.01.T01:intron	40.0%
	CTCCATAATTTAGGGACCAT+GGG	+	Chr4:22269703-22269722	None:intergenic	40.0%
	CTGTTCAACTTGCCAAACTA+GGG	-	Chr4:22270947-22270966	MsG0480019562.01.T01:intron	40.0%
	CTTACCACCAACTCATTTGT+GGG	-	Chr4:22268668-22268687	MsG0480019562.01.T01:intron	40.0%
	CTTGAATTAACACGGATGAG+AGG	+	Chr4:22268073-22268092	None:intergenic	40.0%
	CTTGACTACAAAACTCCAGA+GGG	-	Chr4:22271042-22271061	MsG0480019562.01.T01:intron	40.0%
	GACTGAATCTTCCAATCAAC+TGG	+	Chr4:22266796-22266815	None:intergenic	40.0%
	GAGAGAGAATGAGTCAAAGA+AGG	-	Chr4:22269050-22269069	MsG0480019562.01.T01:intron	40.0%
	GTACACGTAAGAAAAAGTCG+GGG	+	Chr4:22269811-22269830	None:intergenic	40.0%
	GTGCATATGTACCACATGTT+GGG	+	Chr4:22270571-22270590	None:intergenic	40.0%
	GTTGATTGGAAGATTCAGTC+TGG	-	Chr4:22266796-22266815	MsG0480019562.01.T01:CDS	40.0%
	GTTGTAACTGTTAAGCGTGA+GGG	-	Chr4:22271258-22271277	MsG0480019562.01.T01:intron	40.0%
	TAACCGCTTGTAATTGCTGT+AGG	+	Chr4:22268901-22268920	None:intergenic	40.0%
	TAGGATCAGTCATAGAGTAG+TGG	+	Chr4:22270618-22270637	None:intergenic	40.0%
	TATTTGACCCTACCAGACAT+GGG	-	Chr4:22270136-22270155	MsG0480019562.01.T01:intron	40.0%
	TCTCCATAATTTAGGGACCA+TGG	+	Chr4:22269704-22269723	None:intergenic	40.0%
	TCTTGACTACAAAACTCCAG+AGG	-	Chr4:22271041-22271060	MsG0480019562.01.T01:intron	40.0%
	TGATCTTGGGACTTGAATTC+AGG	-	Chr4:22267907-22267926	MsG0480019562.01.T01:intron	40.0%
	TGCATATGTACCACATGTTG+GGG	+	Chr4:22270570-22270589	None:intergenic	40.0%
	TGCTAAGTTTCTGTTTGGGA+GGG	-	Chr4:22267435-22267454	MsG0480019562.01.T01:intron	40.0%
	TGGGAAACCTTCAATGTTAC+CGG	+	Chr4:22270593-22270612	None:intergenic	40.0%
	TGTGTCATACAAGGTATGTG+AGG	+	Chr4:22266856-22266875	None:intergenic	40.0%
	TTATTTGACCCTACCAGACA+TGG	-	Chr4:22270135-22270154	MsG0480019562.01.T01:intron	40.0%
	TTGAACTTTCACAACCACGA+GGG	+	Chr4:22270395-22270414	None:intergenic	40.0%
	TTTGAACTTTCACAACCACG+AGG	+	Chr4:22270396-22270415	None:intergenic	40.0%
!	ACCATGGGAGTGTAAAGAAT+GGG	+	Chr4:22269688-22269707	None:intergenic	40.0%
!	ACCCCCTTTTCACTCAAATT+AGG	+	Chr4:22271150-22271169	None:intergenic	40.0%
!	ATATGCACCGGTAACATTGA+AGG	-	Chr4:22270583-22270602	MsG0480019562.01.T01:intron	40.0%
!	ATGAGATTGGCCGATCAATT+CGG	+	Chr4:22267251-22267270	None:intergenic	40.0%
!	CCAAATTGGCATCGAAAAAC+GGG	+	Chr4:22267076-22267095	None:intergenic	40.0%
!	GGTATGTGAGGAAATTTTCG+AGG	+	Chr4:22266844-22266863	None:intergenic	40.0%
!	GTCCTTCTGTTAGTTTCAGT+CGG	-	Chr4:22268304-22268323	MsG0480019562.01.T01:intron	40.0%
!	GTTAGTTTCAGTCGGTTAAG+TGG	-	Chr4:22268312-22268331	MsG0480019562.01.T01:intron	40.0%
!	GTTTGGAGATGTAGTTTGTG+CGG	-	Chr4:22270280-22270299	MsG0480019562.01.T01:intron	40.0%
!	TTTACCCACAAATGAGTTGG+TGG	+	Chr4:22268675-22268694	None:intergenic	40.0%
!!	ACTTCATTGGTCTTTGGAGT+TGG	+	Chr4:22266742-22266761	None:intergenic	40.0%
!!	CAGTCATAGAGTAGTGGATT+GGG	+	Chr4:22270612-22270631	None:intergenic	40.0%
!!	TCAGTCATAGAGTAGTGGAT+TGG	+	Chr4:22270613-22270632	None:intergenic	40.0%
	AAAGGGAAAAGGGAGGACAT+TGG	+	Chr4:22268853-22268872	None:intergenic	45.0%
	AACAACATGCACTGGAGCTT+TGG	-	Chr4:22270057-22270076	MsG0480019562.01.T01:intron	45.0%
	AATGGTGAGACCTGTTCAAC+AGG	+	Chr4:22268726-22268745	None:intergenic	45.0%
	ACAGGTCTCACCATTTACCA+TGG	-	Chr4:22268731-22268750	MsG0480019562.01.T01:intron	45.0%
	ACATCACTGAAGAGTCCATC+TGG	-	Chr4:22271066-22271085	MsG0480019562.01.T01:CDS	45.0%
	ACATCTCGCTCAACTAGTGA+AGG	-	Chr4:22271287-22271306	MsG0480019562.01.T01:intron	45.0%
	ACCCATTCTTTACACTCCCA+TGG	-	Chr4:22269684-22269703	MsG0480019562.01.T01:intron	45.0%
	AGATTCAGTCTGGTGCTCTA+AGG	-	Chr4:22266806-22266825	MsG0480019562.01.T01:CDS	45.0%
	AGTCACAGCTCACAGGAATA+AGG	+	Chr4:22268632-22268651	None:intergenic	45.0%
	AGTTGACCATGGTATTCCAG+TGG	+	Chr4:22271121-22271140	None:intergenic	45.0%
	ATCATAGACCAGCCATGGTA+TGG	-	Chr4:22269112-22269131	MsG0480019562.01.T01:intron	45.0%
	ATTGCACCACTGGAATACCA+TGG	-	Chr4:22271112-22271131	MsG0480019562.01.T01:CDS	45.0%
	ATTTGACCCTACCAGACATG+GGG	-	Chr4:22270137-22270156	MsG0480019562.01.T01:intron	45.0%
	CGTTGTAACTGTTAAGCGTG+AGG	-	Chr4:22271257-22271276	MsG0480019562.01.T01:intron	45.0%
	CTAAGTTTCTGTTTGGGAGG+GGG	-	Chr4:22267437-22267456	MsG0480019562.01.T01:intron	45.0%
	CTAGCATCAACCCAGTTGAT+TGG	-	Chr4:22266782-22266801	MsG0480019562.01.T01:CDS	45.0%
	CTCCCATGGTCCCTAAATTA+TGG	-	Chr4:22269698-22269717	MsG0480019562.01.T01:intron	45.0%
	CTTAAGATATGTGTGGCTGG+TGG	-	Chr4:22266995-22267014	MsG0480019562.01.T01:CDS	45.0%
	CTTCAGTACAGTTCCTATGG+CGG	-	Chr4:22266569-22266588	MsG0480019562.01.T01:CDS	45.0%
	GAACCTGAATCCAGTTCATG+TGG	+	Chr4:22267217-22267236	None:intergenic	45.0%
	GAGCAAAGCTGAAGATGCTT+GGG	-	Chr4:22271350-22271369	MsG0480019562.01.T01:CDS	45.0%
	GAGTATATCCATACCATGGC+TGG	+	Chr4:22269123-22269142	None:intergenic	45.0%
	GATTCAGTCTGGTGCTCTAA+GGG	-	Chr4:22266807-22266826	MsG0480019562.01.T01:CDS	45.0%
	GCAGTAATACATTGCACCAC+TGG	-	Chr4:22271102-22271121	MsG0480019562.01.T01:CDS	45.0%
	GCATACACAGTGGTTGTCTA+AGG	-	Chr4:22270166-22270185	MsG0480019562.01.T01:intron	45.0%
	GCATCTTCAGCTTTGCTCAA+AGG	+	Chr4:22271348-22271367	None:intergenic	45.0%
	GCTAAGTTTCTGTTTGGGAG+GGG	-	Chr4:22267436-22267455	MsG0480019562.01.T01:intron	45.0%
	GCTCTTCAGTACAGTTCCTA+TGG	-	Chr4:22266566-22266585	MsG0480019562.01.T01:CDS	45.0%
	GCTGTTCAACTTGCCAAACT+AGG	-	Chr4:22270946-22270965	MsG0480019562.01.T01:intron	45.0%
	GCTTACCACCAACTCATTTG+TGG	-	Chr4:22268667-22268686	MsG0480019562.01.T01:intron	45.0%
	GGAATTAAGCTTGATGGGAC+TGG	-	Chr4:22270871-22270890	MsG0480019562.01.T01:intron	45.0%
	GGAGGACATTGGATATTGTG+CGG	+	Chr4:22268842-22268861	None:intergenic	45.0%
	GGTGCATATGTACCACATGT+TGG	+	Chr4:22270572-22270591	None:intergenic	45.0%
	GTACACGTAAGCAAAAGTCG+GGG	-	Chr4:22268376-22268395	MsG0480019562.01.T01:intron	45.0%
	GTGGATCAGTGTGTCATACA+AGG	+	Chr4:22266865-22266884	None:intergenic	45.0%
	GTTGTCGTGGATTTAACACC+TGG	-	Chr4:22271168-22271187	MsG0480019562.01.T01:CDS	45.0%
	TATGCTTGTATCCCCATGTC+TGG	+	Chr4:22270151-22270170	None:intergenic	45.0%
	TATTTCTTCATTGCCCCTCG+TGG	-	Chr4:22270378-22270397	MsG0480019562.01.T01:intron	45.0%
	TCACAGGAATAAGGCCATCA+TGG	+	Chr4:22268623-22268642	None:intergenic	45.0%
	TGAACTTTCACAACCACGAG+GGG	+	Chr4:22270394-22270413	None:intergenic	45.0%
	TGAGCAAAGCTGAAGATGCT+TGG	-	Chr4:22271349-22271368	MsG0480019562.01.T01:CDS	45.0%
	TGCTTATCATAGACCAGCCA+TGG	-	Chr4:22269107-22269126	MsG0480019562.01.T01:intron	45.0%
	TGTGAATGACAGTATGGAGG+TGG	-	Chr4:22270721-22270740	MsG0480019562.01.T01:intron	45.0%
	TTGTATCCCCATGTCTGGTA+GGG	+	Chr4:22270146-22270165	None:intergenic	45.0%
!	ATGGGAGTGTAAAGAATGGG+TGG	+	Chr4:22269685-22269704	None:intergenic	45.0%
!	CTGATTCGAGCGACCAAATT+GGG	-	Chr4:22269973-22269992	MsG0480019562.01.T01:CDS	45.0%
!	GACCATGGGAGTGTAAAGAA+TGG	+	Chr4:22269689-22269708	None:intergenic	45.0%
!	TAGGTTGAAAGGGAAAAGGG+AGG	+	Chr4:22268860-22268879	None:intergenic	45.0%
!	TCTGATTCGAGCGACCAAAT+TGG	-	Chr4:22269972-22269991	MsG0480019562.01.T01:CDS	45.0%
!	TGGCGAGCAGAAGAATGTTT+TGG	-	Chr4:22270891-22270910	MsG0480019562.01.T01:intron	45.0%
!!	AATTCAGGCACTGATGTAGC+AGG	-	Chr4:22267922-22267941	MsG0480019562.01.T01:intron	45.0%
!!	AGTATTGATGGCCATGCTAC+TGG	-	Chr4:22271378-22271397	MsG0480019562.01.T01:five_prime_UTR	45.0%
!!	CCCGTTTTTCGATGCCAATT+TGG	-	Chr4:22267073-22267092	MsG0480019562.01.T01:CDS	45.0%
!!	GCTTGGGCTAAGAGTATTGA+TGG	-	Chr4:22271366-22271385	MsG0480019562.01.T01:exon	45.0%
!!	GTTTTGGTAACTGCTGCTTC+AGG	-	Chr4:22270907-22270926	MsG0480019562.01.T01:intron	45.0%
!!	TAAGAGCTTAGGTGCTGATG+AGG	-	Chr4:22271017-22271036	MsG0480019562.01.T01:intron	45.0%
!!	TTGGTCTTTGGAGTTGGTAC+AGG	+	Chr4:22266736-22266755	None:intergenic	45.0%
!!	ATAATTATTATTAAATATTG+TGG	+	Chr4:22268928-22268947	None:intergenic	5.0%
!!	TAATTATTATTAAATATTGT+GGG	+	Chr4:22268927-22268946	None:intergenic	5.0%
	AACTGTTAAGCGTGAGGGCT+TGG	-	Chr4:22271263-22271282	MsG0480019562.01.T01:intron	50.0%
	ATGCTCTTTGCCCCAACATG+TGG	-	Chr4:22270557-22270576	MsG0480019562.01.T01:intron	50.0%
	CACGTGGTTGTTCCCTAGTT+TGG	+	Chr4:22270962-22270981	None:intergenic	50.0%
	CATCTCCAAACAAGGAGGCA+AGG	+	Chr4:22270271-22270290	None:intergenic	50.0%
	CTCGCTCAACTAGTGAAGGA+TGG	-	Chr4:22271291-22271310	MsG0480019562.01.T01:intron	50.0%
	CTCTTCAGTGATGTTCCCTC+TGG	+	Chr4:22271060-22271079	None:intergenic	50.0%
	CTTGTATCCCCATGTCTGGT+AGG	+	Chr4:22270147-22270166	None:intergenic	50.0%
	GAAGGTAAGGTTAGAGGCAG+AGG	+	Chr4:22270341-22270360	None:intergenic	50.0%
	GCAGGAGAGGTAGTAGAGAT+TGG	-	Chr4:22267940-22267959	MsG0480019562.01.T01:intron	50.0%
	GGAGTGTAAAGAATGGGTGG+TGG	+	Chr4:22269682-22269701	None:intergenic	50.0%
	GGGGATACAAGCATACACAG+TGG	-	Chr4:22270156-22270175	MsG0480019562.01.T01:intron	50.0%
	GGTGCGAGTTTACCTATTGC+CGG	-	Chr4:22270814-22270833	MsG0480019562.01.T01:intron	50.0%
	GTCAACAATGAACTTGGGCC+AGG	+	Chr4:22271189-22271208	None:intergenic	50.0%
	TGGTTCCCAGATGCGCAAAA+TGG	+	Chr4:22269384-22269403	None:intergenic	50.0%
	TGTTAAGCTCTCGCTAGCCA+CGG	+	Chr4:22270761-22270780	None:intergenic	50.0%
	TTGAGAAGCAGCACACTCCA+AGG	+	Chr4:22267034-22267053	None:intergenic	50.0%
!	AACATGCACTGGAGCTTTGG+AGG	-	Chr4:22270060-22270079	MsG0480019562.01.T01:intron	50.0%
!	ATATGTGTGGCTGGTGGTGT+TGG	-	Chr4:22267001-22267020	MsG0480019562.01.T01:CDS	50.0%
!	CAGAGATAGGGGCATGAGAT+TGG	+	Chr4:22267264-22267283	None:intergenic	50.0%
!	TGCACATGAGCACCGTGAAA+GGG	+	Chr4:22268168-22268187	None:intergenic	50.0%
!	TGGGTCCATTTTGCGCATCT+GGG	-	Chr4:22269376-22269395	MsG0480019562.01.T01:intron	50.0%
!	TTCTGTTTGGGAGGGGGTTT+TGG	-	Chr4:22267443-22267462	MsG0480019562.01.T01:intron	50.0%
!	TTGCACATGAGCACCGTGAA+AGG	+	Chr4:22268169-22268188	None:intergenic	50.0%
!	TTGGGTCCATTTTGCGCATC+TGG	-	Chr4:22269375-22269394	MsG0480019562.01.T01:intron	50.0%
!	TTGGTAACTGCTGCTTCAGG+TGG	-	Chr4:22270910-22270929	MsG0480019562.01.T01:intron	50.0%
!!	TTGGAGTGTGCTGCTTCTCA+AGG	-	Chr4:22267033-22267052	MsG0480019562.01.T01:CDS	50.0%
	AGCTGAGACTTCAGGTGGTC+TGG	+	Chr4:22270788-22270807	None:intergenic	55.0%
	CAGTACAGTTCCTATGGCGG+AGG	-	Chr4:22266572-22266591	MsG0480019562.01.T01:CDS	55.0%
	CCTGAAGTCTCAGCTGCTGA+AGG	-	Chr4:22270793-22270812	MsG0480019562.01.T01:intron	55.0%
	CCTTCAGCAGCTGAGACTTC+AGG	+	Chr4:22270796-22270815	None:intergenic	55.0%
	CGGAGGAGTCTCTGGATTGA+AGG	-	Chr4:22266589-22266608	MsG0480019562.01.T01:CDS	55.0%
	CGTGACGCCCTCACAGAAAT+TGG	-	Chr4:22270847-22270866	MsG0480019562.01.T01:intron	55.0%
	GACGCCCTCACAGAAATTGG+AGG	-	Chr4:22270850-22270869	MsG0480019562.01.T01:intron	55.0%
	GAGTGAAAAGGGGGTTGTCG+TGG	-	Chr4:22271155-22271174	MsG0480019562.01.T01:CDS	55.0%
	GATGTTACGAGCACCACAGG+TGG	+	Chr4:22270989-22271008	None:intergenic	55.0%
	GTCGATGTTACGAGCACCAC+AGG	+	Chr4:22270992-22271011	None:intergenic	55.0%
	TCAGCAGCTGAGACTTCAGG+TGG	+	Chr4:22270793-22270812	None:intergenic	55.0%
!	ACTGCTGCTTCAGGTGGTGT+AGG	-	Chr4:22270916-22270935	MsG0480019562.01.T01:intron	55.0%
!!	AGGCACTGATGTAGCAGGAG+AGG	-	Chr4:22267927-22267946	MsG0480019562.01.T01:intron	55.0%
!!	GTGCTGCTTCTCAAGGTCTC+AGG	-	Chr4:22267040-22267059	MsG0480019562.01.T01:CDS	55.0%
!!	TGGACTAGCTGAGTTTGCCG+TGG	-	Chr4:22270741-22270760	MsG0480019562.01.T01:intron	55.0%
!!	TGTTTGGGAGGGGGTTTTGG+AGG	-	Chr4:22267446-22267465	MsG0480019562.01.T01:intron	55.0%
!!!	GTTTGGGAGGGGGTTTTGGA+GGG	-	Chr4:22267447-22267466	MsG0480019562.01.T01:intron	55.0%
	AACCACGTGACAGCCACCTG+TGG	-	Chr4:22270973-22270992	MsG0480019562.01.T01:intron	60.0%
	CGAGCTGTGAGACCGGCAAT+AGG	+	Chr4:22270829-22270848	None:intergenic	60.0%
	TCCAGAGACTCCTCCGCCAT+AGG	+	Chr4:22266585-22266604	None:intergenic	60.0%
!	TCCTATGGCGGAGGAGTCTC+TGG	-	Chr4:22266581-22266600	MsG0480019562.01.T01:CDS	60.0%
!!	GTGGCTGGTGGTGTTGGCTT+GGG	-	Chr4:22267007-22267026	MsG0480019562.01.T01:CDS	60.0%
!!	GTGGTGTTGGCTTGGGACCT+TGG	-	Chr4:22267014-22267033	MsG0480019562.01.T01:CDS	60.0%
!!	TGTGGCTGGTGGTGTTGGCT+TGG	-	Chr4:22267006-22267025	MsG0480019562.01.T01:CDS	60.0%
	CACCACAGGTGGCTGTCACG+TGG	+	Chr4:22270978-22270997	None:intergenic	65.0%
	GGCGTCACGAGCTGTGAGAC+CGG	+	Chr4:22270836-22270855	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
Chr4	gene	22266499	22271422	22266499	ID=MsG0480019562.01;Name=MsG0480019562.01
Chr4	mRNA	22266499	22271422	22266499	ID=MsG0480019562.01.T01;Parent=MsG0480019562.01;Name=MsG0480019562.01.T01;_AED=0.49;_eAED=0.49;_QI=46\|1\|0.75\|1\|1\|1\|4\|0\|330
Chr4	exon	22266499	22267188	22266499	ID=MsG0480019562.01.T01:exon:10583;Parent=MsG0480019562.01.T01
Chr4	exon	22269901	22269992	22269901	ID=MsG0480019562.01.T01:exon:10582;Parent=MsG0480019562.01.T01
Chr4	exon	22271056	22271200	22271056	ID=MsG0480019562.01.T01:exon:10581;Parent=MsG0480019562.01.T01
Chr4	exon	22271311	22271422	22271311	ID=MsG0480019562.01.T01:exon:10580;Parent=MsG0480019562.01.T01
Chr4	five_prime_UTR	22271377	22271422	22271377	ID=MsG0480019562.01.T01:five_prime_utr;Parent=MsG0480019562.01.T01
Chr4	CDS	22271311	22271376	22271311	ID=MsG0480019562.01.T01:cds;Parent=MsG0480019562.01.T01
Chr4	CDS	22271056	22271200	22271056	ID=MsG0480019562.01.T01:cds;Parent=MsG0480019562.01.T01
Chr4	CDS	22269901	22269992	22269901	ID=MsG0480019562.01.T01:cds;Parent=MsG0480019562.01.T01
Chr4	CDS	22266499	22267188	22266499	ID=MsG0480019562.01.T01:cds;Parent=MsG0480019562.01.T01

Gene Sequence

>MsG0480019562.01.T01

ATGGCTGCGAAACTTATGCAAGCTCTTCAGTACAGTTCCTATGGCGGAGGAGTCTCTGGATTGAAGCATGTTGAAGTTCCTGTACCAACTCCAAAGACCAATGAAGTTTTACTCAAATTGGAAGCAACTAGCATCAACCCAGTTGATTGGAAGATTCAGTCTGGTGCTCTAAGGGCTATCTTTTTGCCTCGAAAATTTCCTCACATACCTTGCACTGATGTAGCAGGAGAGGTAGTAGAGATTGGACCACAAGTTAAGGATTTTAAAGCTGGTGACAAAGTTATTGCTAAACTCACACATCAATATGGAGGTGGACTAGCTGAGTTTGCCGTGGCTAGCGAGAGCTTAACAGCTGCCAGACCACCTGAAGTCTCAGCTGCTGAAGGTGCGAGTTTACCTATTGCCGGTCTCACAGCTCGTGACGCCCTCACAGAAATTGGAGGAATTAAGCTTGATGGGACTGGCGAGCAGAAGAATGTTTTGGTAACTGCTGCTTCAGGTGGTGTAGGTTCATTTGCTGTTCAACTTGCCAAACTAGGGAACAACCACGTGACAGCCACCTGTGGTGCTCGTAACATCGACTTTGTTAAGAGCTTAGGTGCTGATGAGGTTCTTGACTACAAAACTCCAGAGGGAACATCACTGAAGAGTCCATCTGGTAAAAAATACGATGCAGTAATACATTGCACCACTGGAATACCATGGTCAACTTTTGATCCTAATTTGAGTGAAAAGGGGGTTGTCGTGGATTTAACACCTGGCCCAAGTTCATTGTTGACTTTTGCACTGAAGAAACTTACTTTTTCAAAGAAGCGATTGGTGCCATTCGTTGTAACTGTTAAGCGTGAGGGCTTGGAACATCTCGCTCAACTAGTGAAGGATGGAAAACTCAAGACAATTATCGACTCTAAGTTTCCTTTGAGCAAAGCTGAAGATGCTTGGGCTAAGAGTATTGATGGCCATGCTACTGGAAAAATCATTGTTGAGCCATAG

Protein sequence

>MsG0480019562.01.T01

MAAKLMQALQYSSYGGGVSGLKHVEVPVPTPKTNEVLLKLEATSINPVDWKIQSGALRAIFLPRKFPHIPCTDVAGEVVEIGPQVKDFKAGDKVIAKLTHQYGGGLAEFAVASESLTAARPPEVSAAEGASLPIAGLTARDALTEIGGIKLDGTGEQKNVLVTAASGGVGSFAVQLAKLGNNHVTATCGARNIDFVKSLGADEVLDYKTPEGTSLKSPSGKKYDAVIHCTTGIPWSTFDPNLSEKGVVVDLTPGPSSLLTFALKKLTFSKKRLVPFVVTVKREGLEHLAQLVKDGKLKTIIDSKFPLSKAEDAWAKSIDGHATGKIIVEP*