Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480018299.01.T01 | AES86511.1 | 98.404 | 564 | 9 | 0 | 1 | 564 | 1 | 564 | 0 | 1137 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480018299.01.T01 | P0C897 | 46.667 | 525 | 239 | 8 | 9 | 528 | 9 | 497 | 7.73E-162 | 474 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480018299.01.T01 | G7JFQ2 | 98.404 | 564 | 9 | 0 | 1 | 564 | 1 | 564 | 0.0 | 1137 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048652.01 | MsG0480018299.01 | 0.801182 | 1.016051e-48 | 4.821039e-46 |
| MsG0480018299.01 | MsG0480022889.01 | 0.809984 | 1.443770e-50 | 8.600406e-48 |
| MsG0480018299.01 | MsG0780039924.01 | 0.827074 | 1.911855e-54 | 1.826243e-51 |
| MsG0480018299.01 | MsG0880042541.01 | 0.801816 | 7.533108e-49 | 3.632136e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480018299.01.T01 | MTR_4g009340 | 98.404 | 564 | 9 | 0 | 1 | 564 | 1 | 564 | 0.0 | 1137 |
| MsG0480018299.01.T01 | MTR_4g009590 | 89.964 | 558 | 54 | 2 | 1 | 557 | 1 | 557 | 0.0 | 1005 |
| MsG0480018299.01.T01 | MTR_4g009620 | 72.939 | 558 | 147 | 4 | 1 | 557 | 1 | 555 | 0.0 | 797 |
| MsG0480018299.01.T01 | MTR_7g017620 | 59.279 | 555 | 214 | 5 | 1 | 554 | 1 | 544 | 0.0 | 674 |
| MsG0480018299.01.T01 | MTR_2g041180 | 31.020 | 245 | 140 | 7 | 21 | 254 | 30 | 256 | 5.53e-20 | 93.6 |
| MsG0480018299.01.T01 | MTR_2g036320 | 31.667 | 240 | 144 | 6 | 21 | 254 | 47 | 272 | 4.66e-19 | 90.9 |
| MsG0480018299.01.T01 | MTR_5g086230 | 32.386 | 176 | 106 | 6 | 43 | 214 | 29 | 195 | 1.51e-17 | 81.6 |
| MsG0480018299.01.T01 | MTR_5g086340 | 33.333 | 189 | 117 | 6 | 373 | 559 | 257 | 438 | 2.86e-16 | 82.0 |
| MsG0480018299.01.T01 | MTR_5g086340 | 26.721 | 247 | 140 | 9 | 18 | 246 | 4 | 227 | 1.09e-13 | 73.9 |
| MsG0480018299.01.T01 | MTR_8g005295 | 29.947 | 187 | 118 | 4 | 25 | 198 | 19 | 205 | 2.04e-15 | 79.3 |
| MsG0480018299.01.T01 | MTR_5g087580 | 28.641 | 206 | 136 | 6 | 354 | 557 | 22 | 218 | 2.40e-15 | 75.9 |
| MsG0480018299.01.T01 | MTR_5g016570 | 30.058 | 173 | 106 | 7 | 21 | 187 | 78 | 241 | 6.18e-15 | 78.2 |
| MsG0480018299.01.T01 | MTR_8g005235 | 32.934 | 167 | 96 | 6 | 42 | 204 | 27 | 181 | 1.66e-14 | 76.3 |
| MsG0480018299.01.T01 | MTR_8g005235 | 29.452 | 146 | 98 | 3 | 395 | 539 | 288 | 429 | 4.96e-12 | 68.6 |
| MsG0480018299.01.T01 | MTR_7g060330 | 24.950 | 497 | 264 | 18 | 59 | 527 | 49 | 464 | 2.07e-14 | 76.3 |
| MsG0480018299.01.T01 | MTR_6g011060 | 25.749 | 167 | 107 | 2 | 42 | 208 | 25 | 174 | 2.99e-14 | 75.5 |
| MsG0480018299.01.T01 | MTR_6g011060 | 26.554 | 177 | 129 | 1 | 375 | 550 | 242 | 418 | 3.95e-11 | 65.9 |
| MsG0480018299.01.T01 | MTR_5g086260 | 28.571 | 182 | 121 | 5 | 379 | 557 | 137 | 312 | 8.78e-14 | 72.8 |
| MsG0480018299.01.T01 | MTR_7g060840 | 28.191 | 188 | 130 | 4 | 372 | 555 | 251 | 437 | 1.78e-13 | 73.2 |
| MsG0480018299.01.T01 | MTR_6g009170 | 25.595 | 168 | 109 | 2 | 40 | 207 | 23 | 174 | 2.73e-13 | 72.4 |
| MsG0480018299.01.T01 | MTR_6g010890 | 28.500 | 200 | 132 | 3 | 358 | 550 | 230 | 425 | 3.55e-13 | 72.0 |
| MsG0480018299.01.T01 | MTR_0209s0060 | 32.075 | 159 | 93 | 6 | 42 | 196 | 29 | 176 | 3.84e-13 | 72.0 |
| MsG0480018299.01.T01 | MTR_0209s0030 | 32.075 | 159 | 93 | 6 | 42 | 196 | 29 | 176 | 3.84e-13 | 72.0 |
| MsG0480018299.01.T01 | MTR_8g005285 | 30.588 | 170 | 105 | 4 | 42 | 198 | 36 | 205 | 3.96e-13 | 72.4 |
| MsG0480018299.01.T01 | MTR_8g005285 | 30.464 | 151 | 96 | 4 | 395 | 540 | 339 | 485 | 8.80e-12 | 68.2 |
| MsG0480018299.01.T01 | MTR_6g010920 | 24.691 | 162 | 105 | 2 | 42 | 203 | 25 | 169 | 4.03e-13 | 71.6 |
| MsG0480018299.01.T01 | MTR_2g035640 | 28.902 | 173 | 117 | 5 | 377 | 546 | 175 | 344 | 1.09e-12 | 70.1 |
| MsG0480018299.01.T01 | MTR_7g060080 | 28.962 | 183 | 125 | 4 | 373 | 551 | 11 | 192 | 1.67e-12 | 67.0 |
| MsG0480018299.01.T01 | MTR_8g005325 | 26.667 | 225 | 146 | 7 | 42 | 258 | 56 | 269 | 1.68e-12 | 70.1 |
| MsG0480018299.01.T01 | MTR_8g005325 | 30.345 | 145 | 98 | 3 | 395 | 539 | 318 | 459 | 2.44e-12 | 69.7 |
| MsG0480018299.01.T01 | MTR_8g005225 | 29.487 | 156 | 106 | 4 | 395 | 550 | 304 | 455 | 1.78e-12 | 70.1 |
| MsG0480018299.01.T01 | MTR_6g011050 | 24.691 | 162 | 107 | 3 | 42 | 203 | 24 | 170 | 1.80e-12 | 69.7 |
| MsG0480018299.01.T01 | MTR_6g009610 | 24.074 | 162 | 106 | 2 | 42 | 203 | 25 | 169 | 1.82e-12 | 69.7 |
| MsG0480018299.01.T01 | MTR_6g009610 | 28.977 | 176 | 122 | 3 | 377 | 550 | 236 | 410 | 4.31e-11 | 65.5 |
| MsG0480018299.01.T01 | MTR_6g009590 | 24.365 | 197 | 128 | 4 | 22 | 218 | 7 | 182 | 1.89e-12 | 69.7 |
| MsG0480018299.01.T01 | MTR_6g009590 | 28.492 | 179 | 127 | 1 | 373 | 550 | 232 | 410 | 4.35e-12 | 68.6 |
| MsG0480018299.01.T01 | MTR_7g059480 | 28.767 | 146 | 102 | 2 | 411 | 555 | 634 | 778 | 2.07e-12 | 70.5 |
| MsG0480018299.01.T01 | MTR_7g060310 | 29.452 | 146 | 101 | 2 | 411 | 555 | 348 | 492 | 4.29e-12 | 68.9 |
| MsG0480018299.01.T01 | MTR_0024s0080 | 25.967 | 181 | 115 | 5 | 43 | 222 | 26 | 188 | 4.48e-12 | 68.6 |
| MsG0480018299.01.T01 | MTR_8g006525 | 31.250 | 160 | 96 | 6 | 42 | 198 | 28 | 176 | 7.06e-12 | 68.2 |
| MsG0480018299.01.T01 | MTR_7g446270 | 23.790 | 496 | 269 | 17 | 59 | 527 | 48 | 461 | 1.35e-11 | 67.4 |
| MsG0480018299.01.T01 | MTR_7g011980 | 28.161 | 174 | 124 | 1 | 378 | 550 | 168 | 341 | 1.56e-11 | 66.6 |
| MsG0480018299.01.T01 | MTR_3g079390 | 28.902 | 173 | 122 | 1 | 379 | 550 | 277 | 449 | 1.71e-11 | 67.0 |
| MsG0480018299.01.T01 | MTR_7g067940 | 31.847 | 157 | 99 | 3 | 41 | 196 | 28 | 177 | 2.58e-11 | 66.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480018299.01.T01 | AT3G02645 | 46.667 | 525 | 239 | 8 | 9 | 528 | 9 | 497 | 7.88e-163 | 474 |
| MsG0480018299.01.T01 | AT3G50180 | 32.922 | 243 | 144 | 11 | 19 | 253 | 153 | 384 | 6.74e-27 | 115 |
| MsG0480018299.01.T01 | AT3G50180 | 31.148 | 183 | 116 | 5 | 377 | 552 | 403 | 582 | 1.04e-13 | 74.3 |
| MsG0480018299.01.T01 | AT3G50120 | 36.364 | 187 | 108 | 7 | 21 | 204 | 80 | 258 | 4.38e-25 | 109 |
| MsG0480018299.01.T01 | AT3G50120 | 29.050 | 179 | 121 | 3 | 377 | 552 | 350 | 525 | 8.41e-13 | 71.2 |
| MsG0480018299.01.T01 | AT3G50170 | 33.333 | 186 | 115 | 6 | 21 | 204 | 90 | 268 | 6.56e-24 | 105 |
| MsG0480018299.01.T01 | AT3G50170 | 33.333 | 186 | 115 | 6 | 21 | 204 | 90 | 268 | 1.85e-23 | 104 |
| MsG0480018299.01.T01 | AT3G50170 | 30.168 | 179 | 119 | 3 | 377 | 552 | 358 | 533 | 3.35e-14 | 75.9 |
| MsG0480018299.01.T01 | AT3G50130 | 32.979 | 188 | 115 | 7 | 21 | 205 | 116 | 295 | 2.67e-21 | 98.2 |
| MsG0480018299.01.T01 | AT3G50140 | 32.258 | 186 | 117 | 6 | 21 | 204 | 90 | 268 | 9.03e-21 | 95.5 |
| MsG0480018299.01.T01 | AT3G50140 | 32.258 | 186 | 117 | 6 | 21 | 204 | 90 | 268 | 2.09e-20 | 95.1 |
| MsG0480018299.01.T01 | AT3G50140 | 26.154 | 195 | 138 | 3 | 362 | 551 | 339 | 532 | 2.51e-12 | 70.1 |
| MsG0480018299.01.T01 | AT3G50150 | 26.493 | 268 | 175 | 9 | 21 | 274 | 67 | 326 | 1.77e-19 | 91.7 |
| MsG0480018299.01.T01 | AT3G50150 | 26.493 | 268 | 175 | 9 | 21 | 274 | 67 | 326 | 1.88e-19 | 92.0 |
| MsG0480018299.01.T01 | AT3G50150 | 30.899 | 178 | 119 | 3 | 377 | 552 | 328 | 503 | 3.49e-16 | 82.0 |
| MsG0480018299.01.T01 | AT5G11290 | 32.903 | 155 | 90 | 3 | 41 | 193 | 32 | 174 | 4.15e-17 | 84.3 |
| MsG0480018299.01.T01 | AT3G50160 | 26.289 | 194 | 122 | 5 | 41 | 228 | 103 | 281 | 4.49e-17 | 84.7 |
| MsG0480018299.01.T01 | AT3G50160 | 29.319 | 191 | 128 | 4 | 364 | 552 | 310 | 495 | 3.60e-14 | 75.5 |
| MsG0480018299.01.T01 | AT4G31980 | 32.278 | 158 | 93 | 4 | 42 | 197 | 293 | 438 | 6.06e-17 | 84.7 |
| MsG0480018299.01.T01 | AT3G47210 | 32.317 | 164 | 102 | 3 | 35 | 198 | 86 | 240 | 1.08e-15 | 80.1 |
| MsG0480018299.01.T01 | AT3G50190 | 30.233 | 172 | 111 | 6 | 21 | 190 | 73 | 237 | 1.90e-15 | 78.6 |
| MsG0480018299.01.T01 | AT3G50190 | 30.233 | 172 | 111 | 6 | 21 | 190 | 73 | 237 | 1.90e-15 | 78.6 |
| MsG0480018299.01.T01 | AT3G50190 | 30.233 | 172 | 111 | 6 | 21 | 190 | 73 | 237 | 2.04e-15 | 78.6 |
| MsG0480018299.01.T01 | AT3G50190 | 30.233 | 172 | 111 | 6 | 21 | 190 | 73 | 237 | 3.28e-15 | 79.0 |
| MsG0480018299.01.T01 | AT3G47250 | 25.342 | 292 | 176 | 6 | 22 | 299 | 36 | 299 | 6.31e-15 | 77.8 |
| MsG0480018299.01.T01 | AT3G47250 | 25.342 | 292 | 176 | 6 | 22 | 299 | 36 | 299 | 6.31e-15 | 77.8 |
| MsG0480018299.01.T01 | AT3G47250 | 25.342 | 292 | 176 | 6 | 22 | 299 | 36 | 299 | 6.31e-15 | 77.8 |
| MsG0480018299.01.T01 | AT2G36430 | 26.009 | 223 | 146 | 4 | 38 | 255 | 41 | 249 | 1.29e-14 | 76.6 |
| MsG0480018299.01.T01 | AT5G22560 | 26.692 | 266 | 132 | 10 | 22 | 252 | 24 | 261 | 1.92e-13 | 73.6 |
| MsG0480018299.01.T01 | AT3G47200 | 29.268 | 164 | 103 | 3 | 35 | 196 | 39 | 191 | 5.59e-13 | 71.6 |
| MsG0480018299.01.T01 | AT3G47200 | 29.268 | 164 | 103 | 3 | 35 | 196 | 39 | 191 | 5.59e-13 | 71.6 |
| MsG0480018299.01.T01 | AT5G22550 | 24.569 | 232 | 145 | 6 | 32 | 252 | 34 | 246 | 6.91e-13 | 71.6 |
| MsG0480018299.01.T01 | AT5G22550 | 28.889 | 180 | 127 | 1 | 377 | 555 | 308 | 487 | 2.43e-12 | 69.7 |
| MsG0480018299.01.T01 | AT5G22550 | 28.889 | 180 | 127 | 1 | 377 | 555 | 259 | 438 | 2.08e-12 | 69.7 |
| MsG0480018299.01.T01 | AT5G22540 | 28.571 | 189 | 110 | 5 | 22 | 198 | 6 | 181 | 9.11e-11 | 64.7 |
Find 77 sgRNAs with CRISPR-Local
Find 95 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAATTGGTCCTTACCATTAT+TGG | 0.181399 | 4:+3092184 | MsG0480018299.01.T01:CDS |
| AGCAATCATTCAATGAAATT+TGG | 0.198118 | 4:+3092853 | MsG0480018299.01.T01:CDS |
| GCAAAGATTCTAAGAGAAAA+AGG | 0.234685 | 4:+3093362 | MsG0480018299.01.T01:CDS |
| TCATTTCCTGTTTCTAATTT+AGG | 0.239490 | 4:-3092744 | None:intergenic |
| CAATGTGCCAAAGCTTCTTA+TGG | 0.260296 | 4:+3092125 | MsG0480018299.01.T01:CDS |
| TATACTTGTAGTCGCTTCTT+TGG | 0.274811 | 4:+3093641 | MsG0480018299.01.T01:CDS |
| CAACCTTTGATGAACTTAGA+TGG | 0.280233 | 4:+3092031 | MsG0480018299.01.T01:CDS |
| CCTCTTCAAATTCTTCTTCA+AGG | 0.288017 | 4:-3092074 | None:intergenic |
| TGCAAGAAGGTGCAACAAAA+AGG | 0.295985 | 4:+3092433 | MsG0480018299.01.T01:CDS |
| TTACCTTCCTATAATTGGTT+TGG | 0.311845 | 4:+3093217 | MsG0480018299.01.T01:CDS |
| CTTTGCATTTCATAGAGTTC+TGG | 0.331900 | 4:-3092210 | None:intergenic |
| ATCAAGCAAATGTGCACTTT+CGG | 0.361310 | 4:-3092700 | None:intergenic |
| TTAACATCCAAACCAATTAT+AGG | 0.368618 | 4:-3093224 | None:intergenic |
| TTTATGAAGTCTTATGTGTT+TGG | 0.373276 | 4:+3093542 | MsG0480018299.01.T01:CDS |
| GTTAAAGAACTTGTGAAGTC+AGG | 0.374433 | 4:+3093128 | MsG0480018299.01.T01:CDS |
| CTCTTCAAATTCTTCTTCAA+GGG | 0.387908 | 4:-3092073 | None:intergenic |
| GACCCATAAAATCTCTCAAA+AGG | 0.390635 | 4:+3092901 | MsG0480018299.01.T01:CDS |
| TTTGTTCATACTTTGTCAAT+TGG | 0.390968 | 4:-3092302 | None:intergenic |
| CTTGGTATGCTTCCATTAGT+AGG | 0.393361 | 4:-3093164 | None:intergenic |
| CGTTCAAATCTAAGAATTTG+TGG | 0.397038 | 4:-3092341 | None:intergenic |
| TGTTGTGGAACGTAAGAATC+TGG | 0.408953 | 4:-3092156 | None:intergenic |
| AAGTATGAACAAAGGGTTAG+AGG | 0.411014 | 4:+3092312 | MsG0480018299.01.T01:CDS |
| TATAATTCCATTCATCAATT+CGG | 0.422039 | 4:-3093324 | None:intergenic |
| ATTGTGCCTAAATTAGAAAC+AGG | 0.430168 | 4:+3092738 | MsG0480018299.01.T01:CDS |
| AGTCTTATGTGTTTGGTTCA+TGG | 0.433433 | 4:+3093549 | MsG0480018299.01.T01:CDS |
| AGAGTTCTGGTCTCCAATAA+TGG | 0.437374 | 4:-3092197 | None:intergenic |
| AAAATCATAAGCAATCTTCC+AGG | 0.447323 | 4:+3092969 | MsG0480018299.01.T01:CDS |
| TCACTAGCCATAAGAAGCTT+TGG | 0.455256 | 4:-3092132 | None:intergenic |
| GTTCCACAACAAGTTGCAAT+TGG | 0.472042 | 4:+3092168 | MsG0480018299.01.T01:CDS |
| CAAATTTATGCAATGCAAGA+AGG | 0.480891 | 4:+3092420 | MsG0480018299.01.T01:CDS |
| TATGAATCATCGGTTGGATC+GGG | 0.482387 | 4:+3093278 | MsG0480018299.01.T01:CDS |
| CACAAATTCTTAGATTTGAA+CGG | 0.491137 | 4:+3092342 | MsG0480018299.01.T01:CDS |
| AAGTGCTTGTAAAGTTTCCA+TGG | 0.492984 | 4:+3092946 | MsG0480018299.01.T01:CDS |
| GGTGAAGATGAAAATTCAAA+AGG | 0.496710 | 4:+3092813 | MsG0480018299.01.T01:CDS |
| GGACCAATTGCAACTTGTTG+TGG | 0.498583 | 4:-3092171 | None:intergenic |
| CTTTCGGAGACTTTAATGCT+TGG | 0.499690 | 4:-3092684 | None:intergenic |
| ATTGACAAAGTATGAACAAA+GGG | 0.500245 | 4:+3092305 | MsG0480018299.01.T01:CDS |
| GCAAGTCATTGAGATTATCA+AGG | 0.501646 | 4:+3093444 | MsG0480018299.01.T01:CDS |
| AACTTTACAAGCACTTTCAA+AGG | 0.504638 | 4:-3092939 | None:intergenic |
| ATCGGTTGGATCGGGACCAT+TGG | 0.507267 | 4:+3093286 | MsG0480018299.01.T01:CDS |
| TTTGCATCGAAATTAATACT+TGG | 0.511465 | 4:-3093182 | None:intergenic |
| TTTAGAATTATATGACATAG+TGG | 0.512393 | 4:-3092007 | None:intergenic |
| TTCACAAGTTCTTTAACAGA+AGG | 0.512475 | 4:-3093122 | None:intergenic |
| AACCTTTGATGAACTTAGAT+GGG | 0.512620 | 4:+3092032 | MsG0480018299.01.T01:CDS |
| TCAAAGGTGGCTTGTTAACA+AGG | 0.514604 | 4:-3093082 | None:intergenic |
| AACTCTTCAATCATCTTGAA+AGG | 0.518028 | 4:-3092660 | None:intergenic |
| GAAGAATTTGAAGAGGAAGA+TGG | 0.518706 | 4:+3092081 | MsG0480018299.01.T01:CDS |
| ACCCATAAAATCTCTCAAAA+GGG | 0.523730 | 4:+3092902 | MsG0480018299.01.T01:CDS |
| TTAAAGAACTTGTGAAGTCA+GGG | 0.526962 | 4:+3093129 | MsG0480018299.01.T01:CDS |
| CTTGGTTGCATATGAATCAT+CGG | 0.528032 | 4:+3093268 | MsG0480018299.01.T01:CDS |
| CAAGTCATTGAGATTATCAA+GGG | 0.529827 | 4:+3093445 | MsG0480018299.01.T01:CDS |
| ATATGAATCATCGGTTGGAT+CGG | 0.532054 | 4:+3093277 | MsG0480018299.01.T01:CDS |
| GTTGCATATGAATCATCGGT+TGG | 0.535467 | 4:+3093272 | MsG0480018299.01.T01:CDS |
| CGTTACACCGAATTGATGAA+TGG | 0.539855 | 4:+3093317 | MsG0480018299.01.T01:CDS |
| ATAAAGAAGTTGCTGATATG+TGG | 0.544942 | 4:+3093411 | MsG0480018299.01.T01:CDS |
| ATAGTGATTTCTTCAATCAA+AGG | 0.550729 | 4:-3093098 | None:intergenic |
| TCACATTTAGTTGACTATGC+TGG | 0.552649 | 4:+3092474 | MsG0480018299.01.T01:CDS |
| TGTTGAGATTCAACAAGAAG+AGG | 0.558169 | 4:+3092779 | MsG0480018299.01.T01:CDS |
| CACCCATCTAAGTTCATCAA+AGG | 0.560565 | 4:-3092034 | None:intergenic |
| TCTGGTCTCCAATAATGGTA+AGG | 0.564617 | 4:-3092192 | None:intergenic |
| CCTTGAAGAAGAATTTGAAG+AGG | 0.574533 | 4:+3092074 | MsG0480018299.01.T01:CDS |
| GTGATTTCTTCAATCAAAGG+TGG | 0.576916 | 4:-3093095 | None:intergenic |
| GAAGTTGCTGATATGTGGAA+TGG | 0.576981 | 4:+3093416 | MsG0480018299.01.T01:CDS |
| ATTCTTTCAAAACTTAACAA+AGG | 0.581545 | 4:+3092879 | MsG0480018299.01.T01:CDS |
| CATCCAAACCAATTATAGGA+AGG | 0.589371 | 4:-3093220 | None:intergenic |
| TGATTTCAAGAATCTTTCTG+TGG | 0.598523 | 4:-3092247 | None:intergenic |
| ACTTTGTCAATTGGTCAACA+AGG | 0.606799 | 4:-3092293 | None:intergenic |
| GTGTAACGCGTTATAACCAA+TGG | 0.610442 | 4:-3093302 | None:intergenic |
| AATTGACAAAGTATGAACAA+AGG | 0.638794 | 4:+3092304 | MsG0480018299.01.T01:CDS |
| AGATTTGAACGGAGAAACGA+TGG | 0.639181 | 4:+3092353 | MsG0480018299.01.T01:CDS |
| CAAGAAGAGGAACAAGACAA+AGG | 0.664135 | 4:+3092792 | MsG0480018299.01.T01:CDS |
| TAAAGAACTTGTGAAGTCAG+GGG | 0.664634 | 4:+3093130 | MsG0480018299.01.T01:CDS |
| GAATGGAATTATAGACTCGG+AGG | 0.673937 | 4:+3093334 | MsG0480018299.01.T01:CDS |
| ATCATAAGCAATCTTCCAGG+AGG | 0.678359 | 4:+3092972 | MsG0480018299.01.T01:CDS |
| CAAAGGTGGCTTGTTAACAA+GGG | 0.680704 | 4:-3093081 | None:intergenic |
| TGAACGGAGAAACGATGGTG+TGG | 0.683030 | 4:+3092358 | MsG0480018299.01.T01:CDS |
| GATGAATGGAATTATAGACT+CGG | 0.696616 | 4:+3093331 | MsG0480018299.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATTTTCTCAAAACAAATAA+CGG | - | Chr4:3092558-3092577 | None:intergenic | 15.0% |
| !!! | TTATTTTTCTCTAAAGAAAA+AGG | + | Chr4:3093023-3093042 | MsG0480018299.01.T01:CDS | 15.0% |
| !! | ATTCTTTCAAAACTTAACAA+AGG | + | Chr4:3092879-3092898 | MsG0480018299.01.T01:CDS | 20.0% |
| !! | GAGAAAAATAAAGATTCAAT+AGG | - | Chr4:3093014-3093033 | None:intergenic | 20.0% |
| !! | TATAATTCCATTCATCAATT+CGG | - | Chr4:3093327-3093346 | None:intergenic | 20.0% |
| !!! | ATGTTGATTTTCATGTTTAT+TGG | + | Chr4:3092618-3092637 | MsG0480018299.01.T01:CDS | 20.0% |
| !!! | ATTTTCTTGTTGTTTTTGAT+GGG | + | Chr4:3093596-3093615 | MsG0480018299.01.T01:CDS | 20.0% |
| !!! | GTTTTATAATAGTAGAATGA+AGG | + | Chr4:3093505-3093524 | MsG0480018299.01.T01:CDS | 20.0% |
| !!! | TATTTTCTTGTTGTTTTTGA+TGG | + | Chr4:3093595-3093614 | MsG0480018299.01.T01:CDS | 20.0% |
| !!! | TTTAGAATTATATGACATAG+TGG | - | Chr4:3092010-3092029 | None:intergenic | 20.0% |
| ! | AATTGACAAAGTATGAACAA+AGG | + | Chr4:3092304-3092323 | MsG0480018299.01.T01:CDS | 25.0% |
| ! | AGCAATCATTCAATGAAATT+TGG | + | Chr4:3092853-3092872 | MsG0480018299.01.T01:CDS | 25.0% |
| ! | ATAGTGATTTCTTCAATCAA+AGG | - | Chr4:3093101-3093120 | None:intergenic | 25.0% |
| ! | ATTGACAAAGTATGAACAAA+GGG | + | Chr4:3092305-3092324 | MsG0480018299.01.T01:CDS | 25.0% |
| ! | CACAAATTCTTAGATTTGAA+CGG | + | Chr4:3092342-3092361 | MsG0480018299.01.T01:CDS | 25.0% |
| ! | TTAACATCCAAACCAATTAT+AGG | - | Chr4:3093227-3093246 | None:intergenic | 25.0% |
| ! | TTTGCATCGAAATTAATACT+TGG | - | Chr4:3093185-3093204 | None:intergenic | 25.0% |
| ! | TTTGTTCATACTTTGTCAAT+TGG | - | Chr4:3092305-3092324 | None:intergenic | 25.0% |
| !! | ACTTTTTACCTTCCTATAAT+TGG | + | Chr4:3093212-3093231 | MsG0480018299.01.T01:CDS | 25.0% |
| !! | CAAAGATTCTAAGAGAAAAA+GGG | + | Chr4:3093363-3093382 | MsG0480018299.01.T01:CDS | 25.0% |
| !! | CAAGTTTCTAAGAAAAACTT+CGG | - | Chr4:3093252-3093271 | None:intergenic | 25.0% |
| !! | GTAAATTTTTCTCCTACTAA+TGG | + | Chr4:3093152-3093171 | MsG0480018299.01.T01:CDS | 25.0% |
| !! | TTTATGAAGTCTTATGTGTT+TGG | + | Chr4:3093542-3093561 | MsG0480018299.01.T01:CDS | 25.0% |
| !!! | TCATTTCCTGTTTCTAATTT+AGG | - | Chr4:3092747-3092766 | None:intergenic | 25.0% |
| AAAATCATAAGCAATCTTCC+AGG | + | Chr4:3092969-3092988 | MsG0480018299.01.T01:CDS | 30.0% | |
| AACCTTTGATGAACTTAGAT+GGG | + | Chr4:3092032-3092051 | MsG0480018299.01.T01:CDS | 30.0% | |
| AACTCTTCAATCATCTTGAA+AGG | - | Chr4:3092663-3092682 | None:intergenic | 30.0% | |
| AACTTTACAAGCACTTTCAA+AGG | - | Chr4:3092942-3092961 | None:intergenic | 30.0% | |
| ACCCATAAAATCTCTCAAAA+GGG | + | Chr4:3092902-3092921 | MsG0480018299.01.T01:CDS | 30.0% | |
| ATAAAGAAGTTGCTGATATG+TGG | + | Chr4:3093411-3093430 | MsG0480018299.01.T01:CDS | 30.0% | |
| ATTGTGCCTAAATTAGAAAC+AGG | + | Chr4:3092738-3092757 | MsG0480018299.01.T01:CDS | 30.0% | |
| CAAATTTATGCAATGCAAGA+AGG | + | Chr4:3092420-3092439 | MsG0480018299.01.T01:CDS | 30.0% | |
| CAAGTCATTGAGATTATCAA+GGG | + | Chr4:3093445-3093464 | MsG0480018299.01.T01:CDS | 30.0% | |
| CGTTCAAATCTAAGAATTTG+TGG | - | Chr4:3092344-3092363 | None:intergenic | 30.0% | |
| CTCTTCAAATTCTTCTTCAA+GGG | - | Chr4:3092076-3092095 | None:intergenic | 30.0% | |
| GATGAATGGAATTATAGACT+CGG | + | Chr4:3093331-3093350 | MsG0480018299.01.T01:CDS | 30.0% | |
| GGTGAAGATGAAAATTCAAA+AGG | + | Chr4:3092813-3092832 | MsG0480018299.01.T01:CDS | 30.0% | |
| TGATTTCAAGAATCTTTCTG+TGG | - | Chr4:3092250-3092269 | None:intergenic | 30.0% | |
| TTAAAGAACTTGTGAAGTCA+GGG | + | Chr4:3093129-3093148 | MsG0480018299.01.T01:CDS | 30.0% | |
| TTACCTTCCTATAATTGGTT+TGG | + | Chr4:3093217-3093236 | MsG0480018299.01.T01:CDS | 30.0% | |
| TTCACAAGTTCTTTAACAGA+AGG | - | Chr4:3093125-3093144 | None:intergenic | 30.0% | |
| ! | AGATTGCTTATGATTTTCCA+TGG | - | Chr4:3092966-3092985 | None:intergenic | 30.0% |
| ! | CGAAGTTTTTCTTAGAAACT+TGG | + | Chr4:3093250-3093269 | MsG0480018299.01.T01:CDS | 30.0% |
| ! | GCAAAGATTCTAAGAGAAAA+AGG | + | Chr4:3093362-3093381 | MsG0480018299.01.T01:CDS | 30.0% |
| !! | ACCCTTTTGAGAGATTTTAT+GGG | - | Chr4:3092906-3092925 | None:intergenic | 30.0% |
| !! | TACCCTTTTGAGAGATTTTA+TGG | - | Chr4:3092907-3092926 | None:intergenic | 30.0% |
| !!! | ATTATCAAGGGTTTTGTTCT+TGG | + | Chr4:3093457-3093476 | MsG0480018299.01.T01:CDS | 30.0% |
| AAGTATGAACAAAGGGTTAG+AGG | + | Chr4:3092312-3092331 | MsG0480018299.01.T01:CDS | 35.0% | |
| AAGTGCTTGTAAAGTTTCCA+TGG | + | Chr4:3092946-3092965 | MsG0480018299.01.T01:CDS | 35.0% | |
| ACTTTGTCAATTGGTCAACA+AGG | - | Chr4:3092296-3092315 | None:intergenic | 35.0% | |
| ATCAAGCAAATGTGCACTTT+CGG | - | Chr4:3092703-3092722 | None:intergenic | 35.0% | |
| CAACCTTTGATGAACTTAGA+TGG | + | Chr4:3092031-3092050 | MsG0480018299.01.T01:CDS | 35.0% | |
| CAATTGGTCCTTACCATTAT+TGG | + | Chr4:3092184-3092203 | MsG0480018299.01.T01:CDS | 35.0% | |
| CATCCAAACCAATTATAGGA+AGG | - | Chr4:3093223-3093242 | None:intergenic | 35.0% | |
| CCTCTTCAAATTCTTCTTCA+AGG | - | Chr4:3092077-3092096 | None:intergenic | 35.0% | |
| CCTTGAAGAAGAATTTGAAG+AGG | + | Chr4:3092074-3092093 | MsG0480018299.01.T01:CDS | 35.0% | |
| CTTGGTTGCATATGAATCAT+CGG | + | Chr4:3093268-3093287 | MsG0480018299.01.T01:CDS | 35.0% | |
| CTTTGCATTTCATAGAGTTC+TGG | - | Chr4:3092213-3092232 | None:intergenic | 35.0% | |
| GAAGAATTTGAAGAGGAAGA+TGG | + | Chr4:3092081-3092100 | MsG0480018299.01.T01:CDS | 35.0% | |
| GACCCATAAAATCTCTCAAA+AGG | + | Chr4:3092901-3092920 | MsG0480018299.01.T01:CDS | 35.0% | |
| GCAAGTCATTGAGATTATCA+AGG | + | Chr4:3093444-3093463 | MsG0480018299.01.T01:CDS | 35.0% | |
| GTGATTTCTTCAATCAAAGG+TGG | - | Chr4:3093098-3093117 | None:intergenic | 35.0% | |
| GTTAAAGAACTTGTGAAGTC+AGG | + | Chr4:3093128-3093147 | MsG0480018299.01.T01:CDS | 35.0% | |
| TAAAGAACTTGTGAAGTCAG+GGG | + | Chr4:3093130-3093149 | MsG0480018299.01.T01:CDS | 35.0% | |
| TATACTTGTAGTCGCTTCTT+TGG | + | Chr4:3093641-3093660 | MsG0480018299.01.T01:CDS | 35.0% | |
| TCACATTTAGTTGACTATGC+TGG | + | Chr4:3092474-3092493 | MsG0480018299.01.T01:CDS | 35.0% | |
| TGTTGAGATTCAACAAGAAG+AGG | + | Chr4:3092779-3092798 | MsG0480018299.01.T01:CDS | 35.0% | |
| ! | AGTCTTATGTGTTTGGTTCA+TGG | + | Chr4:3093549-3093568 | MsG0480018299.01.T01:CDS | 35.0% |
| ! | ATATGAATCATCGGTTGGAT+CGG | + | Chr4:3093277-3093296 | MsG0480018299.01.T01:CDS | 35.0% |
| !!! | TGTTTTAGAAGTTTACCTCC+TGG | - | Chr4:3092990-3093009 | None:intergenic | 35.0% |
| AGATTTGAACGGAGAAACGA+TGG | + | Chr4:3092353-3092372 | MsG0480018299.01.T01:CDS | 40.0% | |
| ATCATAAGCAATCTTCCAGG+AGG | + | Chr4:3092972-3092991 | MsG0480018299.01.T01:CDS | 40.0% | |
| CAAGAAGAGGAACAAGACAA+AGG | + | Chr4:3092792-3092811 | MsG0480018299.01.T01:CDS | 40.0% | |
| CAATGTGCCAAAGCTTCTTA+TGG | + | Chr4:3092125-3092144 | MsG0480018299.01.T01:CDS | 40.0% | |
| CACCCATCTAAGTTCATCAA+AGG | - | Chr4:3092037-3092056 | None:intergenic | 40.0% | |
| CGTTACACCGAATTGATGAA+TGG | + | Chr4:3093317-3093336 | MsG0480018299.01.T01:CDS | 40.0% | |
| CTTGGTATGCTTCCATTAGT+AGG | - | Chr4:3093167-3093186 | None:intergenic | 40.0% | |
| CTTTCGGAGACTTTAATGCT+TGG | - | Chr4:3092687-3092706 | None:intergenic | 40.0% | |
| GAAGTTGCTGATATGTGGAA+TGG | + | Chr4:3093416-3093435 | MsG0480018299.01.T01:CDS | 40.0% | |
| GAATGGAATTATAGACTCGG+AGG | + | Chr4:3093334-3093353 | MsG0480018299.01.T01:CDS | 40.0% | |
| GTGTAACGCGTTATAACCAA+TGG | - | Chr4:3093305-3093324 | None:intergenic | 40.0% | |
| GTTCCACAACAAGTTGCAAT+TGG | + | Chr4:3092168-3092187 | MsG0480018299.01.T01:CDS | 40.0% | |
| GTTGCATATGAATCATCGGT+TGG | + | Chr4:3093272-3093291 | MsG0480018299.01.T01:CDS | 40.0% | |
| TCACTAGCCATAAGAAGCTT+TGG | - | Chr4:3092135-3092154 | None:intergenic | 40.0% | |
| TCTGGTCTCCAATAATGGTA+AGG | - | Chr4:3092195-3092214 | None:intergenic | 40.0% | |
| TGTTGTGGAACGTAAGAATC+TGG | - | Chr4:3092159-3092178 | None:intergenic | 40.0% | |
| ! | AGAGTTCTGGTCTCCAATAA+TGG | - | Chr4:3092200-3092219 | None:intergenic | 40.0% |
| ! | GCAAGAAGGTGCAACAAAAA+GGG | + | Chr4:3092434-3092453 | MsG0480018299.01.T01:CDS | 40.0% |
| ! | TATGAATCATCGGTTGGATC+GGG | + | Chr4:3093278-3093297 | MsG0480018299.01.T01:CDS | 40.0% |
| ! | TGCAAGAAGGTGCAACAAAA+AGG | + | Chr4:3092433-3092452 | MsG0480018299.01.T01:CDS | 40.0% |
| !! | CAAAGGTGGCTTGTTAACAA+GGG | - | Chr4:3093084-3093103 | None:intergenic | 40.0% |
| !! | TCAAAGGTGGCTTGTTAACA+AGG | - | Chr4:3093085-3093104 | None:intergenic | 40.0% |
| !! | GGACCAATTGCAACTTGTTG+TGG | - | Chr4:3092174-3092193 | None:intergenic | 45.0% |
| TGAACGGAGAAACGATGGTG+TGG | + | Chr4:3092358-3092377 | MsG0480018299.01.T01:CDS | 50.0% | |
| ATCGGTTGGATCGGGACCAT+TGG | + | Chr4:3093286-3093305 | MsG0480018299.01.T01:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 3091991 | 3093685 | 3091991 | ID=MsG0480018299.01;Name=MsG0480018299.01 |
| Chr4 | mRNA | 3091991 | 3093685 | 3091991 | ID=MsG0480018299.01.T01;Parent=MsG0480018299.01;Name=MsG0480018299.01.T01;_AED=0.03;_eAED=0.03;_QI=0|-1|0|1|-1|1|1|0|564 |
| Chr4 | exon | 3091991 | 3093685 | 3091991 | ID=MsG0480018299.01.T01:exon:1305;Parent=MsG0480018299.01.T01 |
| Chr4 | CDS | 3091991 | 3093685 | 3091991 | ID=MsG0480018299.01.T01:cds;Parent=MsG0480018299.01.T01 |
| Gene Sequence |
| Protein sequence |
