AlfalfaGEDB | MsG0480019218.01

M. sativa cultivar ZhongmuNo.1 / MsG0480019218.01

KEGG: K14488
GO: GO:0009733
PFAM: PF02519

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480019218.01.T01	XP_013455080.1	92.683	164	9	1	1	164	1	161	3.09E-106	310

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480019218.01.T01	O22150	51.829	164	64	5	3	162	7	159	3.39E-49	159

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480019218.01.T01	I3T7X1	92.683	164	9	1	1	164	1	161	1.48e-106	310

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0480019218.01	MsG0480023937.01	0.806783	6.956973e-50	3.809480e-47
MsG0480019218.01	MsG0580024996.01	0.811246	7.705237e-51	4.745450e-48
MsG0480019218.01	MsG0780038092.01	0.806695	7.261799e-50	3.967384e-47
MsG0480019218.01	MsG0880042743.01	0.823474	1.358298e-53	1.170716e-50
MsG0480019218.01	MsG0880043059.01	0.803518	3.355107e-49	1.689539e-46
MsG0280008295.01	MsG0480019218.01	0.806809	6.868547e-50	3.763801e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480019218.01.T01	MTR_4g025830	92.683	164	9	1	1	164	1	161	3.75e-110	310
MsG0480019218.01.T01	MTR_0388s0020	51.333	150	62	3	3	152	7	145	6.79e-46	147
MsG0480019218.01.T01	MTR_7g118010	53.571	84	35	2	82	165	23	102	5.94e-23	87.8
MsG0480019218.01.T01	MTR_3g084250	55.882	68	26	2	82	148	25	89	4.74e-21	82.4
MsG0480019218.01.T01	MTR_3g092220	50.000	84	37	2	82	165	33	111	3.95e-20	80.9
MsG0480019218.01.T01	MTR_8g026730	51.190	84	36	2	82	165	27	105	1.10e-19	79.3
MsG0480019218.01.T01	MTR_3g084210	57.377	61	22	2	82	141	25	82	1.02e-18	76.6
MsG0480019218.01.T01	MTR_3g084230	57.377	61	22	2	82	141	25	82	1.02e-18	76.6
MsG0480019218.01.T01	MTR_4g072220	50.000	72	32	2	79	149	23	91	1.09e-18	76.6
MsG0480019218.01.T01	MTR_4g072240	50.704	71	31	2	79	148	23	90	1.59e-18	75.9
MsG0480019218.01.T01	MTR_3g084160	57.377	61	22	2	82	141	25	82	1.69e-18	75.9
MsG0480019218.01.T01	MTR_4g072300	50.704	71	31	2	79	148	23	90	3.28e-18	75.1
MsG0480019218.01.T01	MTR_8g461420	55.738	61	23	2	82	141	25	82	7.15e-18	74.3
MsG0480019218.01.T01	MTR_3g084190	55.738	61	23	2	82	141	25	82	1.35e-17	73.6
MsG0480019218.01.T01	MTR_3g110045	55.738	61	23	2	82	141	84	141	3.41e-17	74.3
MsG0480019218.01.T01	MTR_4g072570	48.529	68	31	2	82	148	25	89	4.08e-17	72.4
MsG0480019218.01.T01	MTR_4g072980	45.333	75	38	2	77	150	22	94	1.17e-16	71.2
MsG0480019218.01.T01	MTR_4g072850	48.529	68	31	2	82	148	27	91	1.26e-16	71.2
MsG0480019218.01.T01	MTR_4g072500	50.000	66	29	2	82	146	25	87	2.15e-16	70.5
MsG0480019218.01.T01	MTR_4g072330	47.059	68	32	2	82	148	23	87	2.65e-16	70.1
MsG0480019218.01.T01	MTR_3g117640	48.571	70	33	2	82	150	34	101	2.87e-16	70.5
MsG0480019218.01.T01	MTR_4g072530	51.515	66	28	2	82	146	25	87	3.57e-16	70.1
MsG0480019218.01.T01	MTR_4g072910	48.529	68	31	2	82	148	35	99	3.91e-16	70.1
MsG0480019218.01.T01	MTR_4g072230	46.269	67	30	2	82	147	26	87	4.26e-16	69.7
MsG0480019218.01.T01	MTR_4g072890	47.887	71	33	2	79	148	23	90	4.90e-16	69.7
MsG0480019218.01.T01	MTR_4g072830	41.772	79	42	2	71	148	12	87	6.58e-16	69.3
MsG0480019218.01.T01	MTR_4g072250	45.588	68	32	2	82	148	26	89	6.64e-16	69.3
MsG0480019218.01.T01	MTR_4g072640	48.485	66	30	2	82	146	25	87	8.68e-16	68.9
MsG0480019218.01.T01	MTR_4g072260	45.833	72	35	2	79	149	23	91	1.21e-15	68.6
MsG0480019218.01.T01	MTR_4g072820	47.059	68	32	2	82	148	20	84	1.81e-15	68.2
MsG0480019218.01.T01	MTR_4g072310	45.588	68	33	2	82	148	26	90	2.19e-15	68.2
MsG0480019218.01.T01	MTR_4g072370	45.070	71	35	2	79	148	20	87	2.31e-15	67.8
MsG0480019218.01.T01	MTR_4g072870	46.479	71	34	2	79	148	23	90	2.37e-15	67.8
MsG0480019218.01.T01	MTR_3g117610	50.000	60	27	1	82	141	45	101	2.66e-15	68.2
MsG0480019218.01.T01	MTR_4g072880	47.059	68	32	2	82	148	26	90	2.89e-15	67.8
MsG0480019218.01.T01	MTR_4g072160	50.794	63	28	1	82	144	37	96	3.23e-15	68.9
MsG0480019218.01.T01	MTR_4g072190	44.776	67	34	1	82	148	36	99	4.01e-15	67.4
MsG0480019218.01.T01	MTR_4g072600	44.444	72	36	2	79	149	17	85	9.06e-15	66.2
MsG0480019218.01.T01	MTR_4g072670	47.059	68	32	2	82	148	21	85	1.22e-14	65.9
MsG0480019218.01.T01	MTR_4g072930	46.479	71	36	2	79	147	38	108	1.34e-14	66.6
MsG0480019218.01.T01	MTR_4g072860	46.970	66	31	2	82	146	26	88	1.63e-14	65.9
MsG0480019218.01.T01	MTR_4g072580	41.667	72	38	2	79	149	17	85	2.09e-14	65.5
MsG0480019218.01.T01	MTR_2g066830	42.105	76	41	1	82	157	58	130	2.18e-14	66.6
MsG0480019218.01.T01	MTR_2g043960	50.000	68	28	2	77	141	68	132	2.31e-14	67.0
MsG0480019218.01.T01	MTR_3g113310	38.298	94	46	3	57	148	22	105	2.43e-14	65.5
MsG0480019218.01.T01	MTR_4g072940	47.059	68	32	2	82	148	57	121	2.69e-14	65.9
MsG0480019218.01.T01	MTR_4g124750	51.667	60	26	1	82	141	69	125	3.06e-14	66.6
MsG0480019218.01.T01	MTR_3g117630	47.761	67	31	2	82	147	25	88	3.75e-14	64.7
MsG0480019218.01.T01	MTR_8g461210	39.744	78	44	1	80	157	33	107	4.23e-14	65.5
MsG0480019218.01.T01	MTR_4g072560	44.444	72	37	2	78	148	28	97	5.76e-14	64.3
MsG0480019218.01.T01	MTR_8g076040	46.269	67	33	1	82	148	41	104	6.06e-14	64.7
MsG0480019218.01.T01	MTR_3g084150	44.776	67	33	2	82	147	25	88	6.46e-14	64.3
MsG0480019218.01.T01	MTR_3g084180	44.776	67	33	2	82	147	25	88	7.82e-14	63.9
MsG0480019218.01.T01	MTR_4g072610	43.056	72	37	2	79	149	16	84	1.01e-13	63.5
MsG0480019218.01.T01	MTR_4g072590	50.820	61	26	2	82	141	20	77	1.02e-13	63.5
MsG0480019218.01.T01	MTR_5g021820	43.284	67	35	1	82	148	40	103	1.02e-13	63.9
MsG0480019218.01.T01	MTR_4g072480	46.970	66	31	2	82	146	25	87	1.09e-13	63.5
MsG0480019218.01.T01	MTR_1g061570	41.558	77	42	1	80	156	17	90	1.30e-13	64.3
MsG0480019218.01.T01	MTR_4g072630	45.070	71	35	2	79	148	18	85	1.38e-13	63.2
MsG0480019218.01.T01	MTR_1g009150	44.595	74	37	2	71	144	61	130	1.57e-13	65.5
MsG0480019218.01.T01	MTR_4g072720	45.588	68	33	2	82	148	21	85	1.84e-13	62.8
MsG0480019218.01.T01	MTR_1g024085	44.737	76	38	2	80	154	35	107	1.84e-13	64.3
MsG0480019218.01.T01	MTR_4g072730	46.970	66	31	2	82	146	25	87	1.96e-13	62.8
MsG0480019218.01.T01	MTR_4g072270	46.970	66	31	2	82	146	26	88	2.30e-13	62.8
MsG0480019218.01.T01	MTR_4g005320	47.619	63	30	1	82	144	70	129	2.61e-13	64.3
MsG0480019218.01.T01	MTR_4g072490	44.118	68	34	2	82	148	20	84	2.74e-13	62.4
MsG0480019218.01.T01	MTR_8g461410	43.284	67	34	2	82	147	25	88	2.81e-13	62.4
MsG0480019218.01.T01	MTR_4g072510	43.662	71	36	2	79	148	18	85	3.79e-13	62.0
MsG0480019218.01.T01	MTR_4g072660	46.970	66	31	2	82	146	20	82	4.23e-13	62.0
MsG0480019218.01.T01	MTR_5g008370	43.478	69	35	2	79	146	22	87	4.71e-13	63.2
MsG0480019218.01.T01	MTR_4g072620	45.455	66	32	2	82	146	25	87	4.74e-13	62.0
MsG0480019218.01.T01	MTR_4g072680	44.118	68	34	2	82	148	25	89	7.73e-13	61.2
MsG0480019218.01.T01	MTR_3g110042	45.588	68	33	2	82	148	25	89	9.05e-13	61.2
MsG0480019218.01.T01	MTR_1g059770	38.095	84	49	1	76	159	43	123	9.60e-13	62.0
MsG0480019218.01.T01	MTR_4g072460	44.118	68	34	2	82	148	25	89	9.97e-13	61.2
MsG0480019218.01.T01	MTR_4g072550	44.776	67	33	2	82	147	36	99	1.02e-12	61.2
MsG0480019218.01.T01	MTR_5g008360	41.176	68	36	2	82	148	16	80	1.14e-12	60.8
MsG0480019218.01.T01	MTR_4g072650	41.667	72	38	2	79	149	16	84	2.04e-12	60.1
MsG0480019218.01.T01	MTR_4g050700	46.970	66	31	2	82	146	26	88	2.34e-12	60.1
MsG0480019218.01.T01	MTR_2g012410	46.970	66	31	2	82	146	26	88	2.34e-12	60.1
MsG0480019218.01.T01	MTR_4g072450	42.647	68	35	2	82	148	20	84	2.54e-12	59.7
MsG0480019218.01.T01	MTR_8g096440	37.113	97	57	2	58	153	20	113	3.36e-12	60.8
MsG0480019218.01.T01	MTR_3g109160	42.105	76	40	2	78	153	65	136	6.49e-12	60.8
MsG0480019218.01.T01	MTR_5g008350	48.276	58	26	2	83	139	21	75	8.48e-12	58.2
MsG0480019218.01.T01	MTR_4g072470	41.176	68	36	2	82	148	20	84	9.48e-12	58.2
MsG0480019218.01.T01	MTR_4g057810	34.940	83	50	2	78	157	34	115	1.37e-11	58.9
MsG0480019218.01.T01	MTR_4g072770	40.000	70	38	2	82	150	15	81	1.40e-11	57.8
MsG0480019218.01.T01	MTR_7g405740	44.444	63	32	1	82	144	67	126	1.80e-11	59.3
MsG0480019218.01.T01	MTR_4g072280	53.061	49	20	1	79	127	23	68	2.25e-11	57.4
MsG0480019218.01.T01	MTR_4g072465	47.368	57	26	2	82	137	21	74	3.18e-11	57.0
MsG0480019218.01.T01	MTR_4g072750	49.057	53	24	1	79	131	24	73	3.92e-11	56.6
MsG0480019218.01.T01	MTR_4g072690	39.706	68	37	2	82	148	20	84	4.50e-11	56.6
MsG0480019218.01.T01	MTR_2g095430	40.625	64	37	1	82	144	45	108	4.96e-11	57.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480019218.01.T01	AT3G60690	52.597	154	67	4	3	152	7	158	7.31e-52	163
MsG0480019218.01.T01	AT2G45210	51.829	164	64	5	3	162	7	159	3.46e-50	159
MsG0480019218.01.T01	AT4G12410	45.513	156	71	5	3	156	7	150	1.74e-35	121
MsG0480019218.01.T01	AT4G22620	43.871	155	78	4	3	156	7	153	5.07e-33	115
MsG0480019218.01.T01	AT4G00880	58.904	73	29	1	76	148	22	93	2.61e-21	84.3
MsG0480019218.01.T01	AT2G46690	61.194	67	26	0	82	148	24	90	3.02e-21	84.0
MsG0480019218.01.T01	AT3G61900	59.701	67	27	0	82	148	30	96	1.14e-20	82.8
MsG0480019218.01.T01	AT5G53590	53.247	77	34	1	82	156	46	122	7.68e-20	80.9
MsG0480019218.01.T01	AT2G21210	50.000	72	34	2	82	152	28	98	8.04e-18	74.3
MsG0480019218.01.T01	AT2G21210	50.000	72	34	2	82	152	95	165	1.74e-17	75.5
MsG0480019218.01.T01	AT4G34800	50.685	73	35	1	70	141	9	81	1.85e-17	73.6
MsG0480019218.01.T01	AT4G38850	44.000	75	41	1	75	148	14	88	3.71e-17	72.4
MsG0480019218.01.T01	AT4G34790	59.016	61	23	2	82	141	39	98	1.00e-16	72.0
MsG0480019218.01.T01	AT4G34810	54.839	62	26	2	82	141	48	109	1.43e-16	71.6
MsG0480019218.01.T01	AT3G43120	52.941	68	26	2	77	141	71	135	4.26e-16	71.6
MsG0480019218.01.T01	AT5G18080	50.000	72	33	2	78	148	20	89	4.64e-16	69.7
MsG0480019218.01.T01	AT3G03820	53.731	67	30	1	83	148	28	94	5.07e-16	69.7
MsG0480019218.01.T01	AT3G20220	44.578	83	41	3	66	147	32	110	8.26e-16	69.7
MsG0480019218.01.T01	AT3G03850	46.250	80	40	3	70	148	14	91	1.22e-15	68.6
MsG0480019218.01.T01	AT4G34780	48.529	68	34	1	82	148	28	95	1.28e-15	68.9
MsG0480019218.01.T01	AT4G34770	50.000	72	33	3	82	152	35	104	1.53e-15	68.9
MsG0480019218.01.T01	AT5G10990	45.349	86	41	2	80	162	39	121	1.73e-15	69.7
MsG0480019218.01.T01	AT5G18010	47.222	72	35	2	78	148	20	89	1.93e-15	68.2
MsG0480019218.01.T01	AT2G21220	44.000	75	37	2	76	148	32	103	2.24e-15	68.2
MsG0480019218.01.T01	AT4G38825	49.254	67	31	2	83	148	24	88	3.35e-15	67.4
MsG0480019218.01.T01	AT4G38840	49.254	67	31	2	82	147	33	97	3.38e-15	67.8
MsG0480019218.01.T01	AT5G20810	51.471	68	27	2	77	141	71	135	3.41e-15	69.3
MsG0480019218.01.T01	AT5G18020	47.887	71	34	2	78	147	20	88	5.02e-15	67.0
MsG0480019218.01.T01	AT5G18050	48.571	70	33	2	80	148	22	89	5.69e-15	67.0
MsG0480019218.01.T01	AT5G20810	51.471	68	27	2	77	141	71	135	7.00e-15	69.3
MsG0480019218.01.T01	AT1G56150	42.500	80	43	1	76	155	32	108	2.66e-14	65.9
MsG0480019218.01.T01	AT5G18060	49.254	67	31	2	83	148	25	89	2.80e-14	65.1
MsG0480019218.01.T01	AT5G18030	45.833	72	36	2	78	148	18	87	3.16e-14	65.1
MsG0480019218.01.T01	AT3G03840	50.746	67	31	2	83	148	28	93	3.68e-14	65.1
MsG0480019218.01.T01	AT4G34760	44.776	67	34	1	82	148	43	106	4.54e-14	65.1
MsG0480019218.01.T01	AT1G75580	38.889	108	55	5	46	148	6	107	1.36e-13	63.9
MsG0480019218.01.T01	AT1G75590	49.206	63	29	1	80	142	40	99	1.98e-13	64.7
MsG0480019218.01.T01	AT2G21200	46.269	67	33	2	83	148	22	86	2.98e-13	62.4
MsG0480019218.01.T01	AT4G38860	44.737	76	36	3	76	148	32	104	3.20e-13	62.8
MsG0480019218.01.T01	AT4G34750	45.946	74	33	2	71	144	30	96	3.30e-13	63.9
MsG0480019218.01.T01	AT4G34750	45.946	74	33	2	71	144	30	96	3.30e-13	63.9
MsG0480019218.01.T01	AT4G13790	47.761	67	33	2	83	148	26	91	5.88e-13	61.6
MsG0480019218.01.T01	AT1G19830	43.284	67	35	1	82	148	48	111	1.58e-12	61.2
MsG0480019218.01.T01	AT3G12830	41.667	72	39	1	80	151	48	116	1.67e-12	61.6
MsG0480019218.01.T01	AT3G03830	45.714	70	37	1	80	148	22	91	1.80e-12	60.5
MsG0480019218.01.T01	AT1G19840	47.619	63	30	1	80	142	39	98	2.10e-12	61.6
MsG0480019218.01.T01	AT4G36110	46.774	62	30	1	80	141	38	96	3.19e-12	60.1
MsG0480019218.01.T01	AT5G66260	37.838	74	43	1	75	148	27	97	3.90e-12	59.7
MsG0480019218.01.T01	AT1G16510	42.857	70	37	1	82	151	54	120	5.56e-12	60.5
MsG0480019218.01.T01	AT2G24400	47.619	63	30	1	82	144	64	123	2.31e-11	59.7
MsG0480019218.01.T01	AT2G18010	49.091	55	25	1	82	136	48	99	2.95e-11	57.8
MsG0480019218.01.T01	AT4G31320	43.077	65	34	1	80	144	78	139	3.72e-11	59.3
MsG0480019218.01.T01	AT2G16580	33.663	101	56	2	48	148	18	107	4.81e-11	57.0
MsG0480019218.01.T01	AT2G37030	42.857	63	33	1	82	144	49	108	7.75e-11	57.0

Find 65 sgRNAs with CRISPR-Local

Find 69 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TACTTCAATCATCCTTTGTT+TGG	0.205259	4:-16346664	MsG0480019218.01.T01:CDS
CGGTGAGTTTCGGCGGGTTT+TGG	0.248448	4:-16346698	MsG0480019218.01.T01:CDS
ACTTCAATCATCCTTTGTTT+GGG	0.255039	4:-16346663	MsG0480019218.01.T01:CDS
AAAGCAGGGTCGGGTAAATT+TGG	0.257203	4:-16346808	MsG0480019218.01.T01:CDS
AAGTGATTCGGGTCAGTAAA+TGG	0.277611	4:-16346960	MsG0480019218.01.T01:CDS
AATGGATATTCCGGCGATTT+CGG	0.281583	4:-16346942	MsG0480019218.01.T01:CDS
AAAGAAGCTGAGAAAGAGTA+TGG	0.306967	4:-16346631	MsG0480019218.01.T01:CDS
TATCCGACCCAGCTGCTATT+CGG	0.329644	4:+16346545	None:intergenic
GGTGAACCGCTTTGCTCAAC+CGG	0.334212	4:+16346763	None:intergenic
ATCCGACCCAGCTGCTATTC+GGG	0.371460	4:+16346546	None:intergenic
CGAGACGACGGTATCTTGTT+CGG	0.398391	4:+16346917	None:intergenic
TCGGGTTACCGAGTTTGAAC+GGG	0.414517	4:-16346575	MsG0480019218.01.T01:CDS
GTCGGGTTACCGAGTTTGAA+CGG	0.425737	4:-16346576	MsG0480019218.01.T01:CDS
GCTAGGGAAGAAAGTGATTC+GGG	0.437214	4:-16346971	MsG0480019218.01.T01:CDS
AGCTAGGGAAGAAAGTGATT+CGG	0.439484	4:-16346972	MsG0480019218.01.T01:CDS
CTTCCCTAGCTTGAATTGCA+TGG	0.441083	4:+16346984	None:intergenic
GTCGTCAAATGATTCTTCTC+CGG	0.445127	4:+16346886	None:intergenic
GAGACGACGGTATCTTGTTC+GGG	0.448535	4:+16346918	None:intergenic
GGGTCAGTAAATGGATATTC+CGG	0.450545	4:-16346951	MsG0480019218.01.T01:CDS
TGGAATCACTATTCCTTGTC+GGG	0.453830	4:-16346593	MsG0480019218.01.T01:CDS
GTGGAATCACTATTCCTTGT+CGG	0.471607	4:-16346594	MsG0480019218.01.T01:CDS
GGGCATTTAGCGGTGTATGT+GGG	0.472930	4:-16346730	MsG0480019218.01.T01:CDS
AAGTCTCTATGTTCAAAAGC+AGG	0.487934	4:-16346823	MsG0480019218.01.T01:CDS
AATTTGGTTATTTGCCGATA+GGG	0.495078	4:-16346792	MsG0480019218.01.T01:CDS
CCGTCGTCTCGGCGCCTCAC+CGG	0.497600	4:-16346905	MsG0480019218.01.T01:CDS
GGGGCATTTAGCGGTGTATG+TGG	0.497853	4:-16346731	MsG0480019218.01.T01:CDS
GCTAAATGCCCCTTCGGTAC+CGG	0.500069	4:+16346742	None:intergenic
TACACCGCTAAATGCCCCTT+CGG	0.500996	4:+16346736	None:intergenic
GTTAAGACCCGAATAGCAGC+TGG	0.501036	4:-16346553	MsG0480019218.01.T01:CDS
TGACGACAAAGCTTCTATCA+TGG	0.502757	4:-16346870	MsG0480019218.01.T01:CDS
GACGACAAAGCTTCTATCAT+GGG	0.503605	4:-16346869	MsG0480019218.01.T01:CDS
AAATTTGGTTATTTGCCGAT+AGG	0.508006	4:-16346793	MsG0480019218.01.T01:CDS
TGGGTCGGATACGCGAAGAT+TGG	0.508589	4:-16346533	MsG0480019218.01.T01:CDS
ATAACCATGCAATTCAAGCT+AGG	0.509622	4:-16346988	None:intergenic
GGTCTTAACCCGTTCAAACT+CGG	0.514111	4:+16346567	None:intergenic
CTATGTTCAAAAGCAGGGTC+GGG	0.516192	4:-16346817	MsG0480019218.01.T01:CDS
CTTCAATCATCCTTTGTTTG+GGG	0.517007	4:-16346662	MsG0480019218.01.T01:CDS
AAGCGGTTCACCGGTACCGA+AGG	0.543995	4:-16346752	MsG0480019218.01.T01:CDS
GGCATTTAGCGGTGTATGTG+GGG	0.555901	4:-16346729	MsG0480019218.01.T01:CDS
AAAAGACGGTGAGTTTCGGC+GGG	0.560575	4:-16346704	MsG0480019218.01.T01:CDS
TCTATGTTCAAAAGCAGGGT+CGG	0.560582	4:-16346818	MsG0480019218.01.T01:CDS
TTGTTCGGGTCCGAAATCGC+CGG	0.561790	4:+16346932	None:intergenic
TAACCATGCAATTCAAGCTA+GGG	0.566394	4:-16346987	None:intergenic
GGTTGAGCAAAGCGGTTCAC+CGG	0.573801	4:-16346761	MsG0480019218.01.T01:CDS
TTCAACAACTCCCCAAACAA+AGG	0.574136	4:+16346652	None:intergenic
GACCCGAATAGCAGCTGGGT+CGG	0.582499	4:-16346548	MsG0480019218.01.T01:CDS
AGCGGTTCACCGGTACCGAA+GGG	0.590068	4:-16346751	MsG0480019218.01.T01:CDS
GGTACCGAAGGGGCATTTAG+CGG	0.599134	4:-16346740	MsG0480019218.01.T01:CDS
CCGGTGAGGCGCCGAGACGA+CGG	0.603271	4:+16346905	None:intergenic
AAGAAGCTGAGAAAGAGTAT+GGG	0.603282	4:-16346630	MsG0480019218.01.T01:CDS
ACCGCTTTGCTCAACCGGGT+CGG	0.604772	4:+16346768	None:intergenic
TCAACCGGGTCGGACCCTAT+CGG	0.605566	4:+16346778	None:intergenic
GTGAACCGCTTTGCTCAACC+GGG	0.606769	4:+16346764	None:intergenic
ACGACAAAGCTTCTATCATG+GGG	0.613722	4:-16346868	MsG0480019218.01.T01:CDS
CGAACAAGATACCGTCGTCT+CGG	0.613951	4:-16346916	MsG0480019218.01.T01:CDS
AGTCTCTATGTTCAAAAGCA+GGG	0.613958	4:-16346822	MsG0480019218.01.T01:CDS
TCAAACTCGGTAACCCGACA+AGG	0.618391	4:+16346580	None:intergenic
CAAATGATTCTTCTCCGGTG+AGG	0.645717	4:+16346891	None:intergenic
TTTGCCGATAGGGTCCGACC+CGG	0.646098	4:-16346782	MsG0480019218.01.T01:CDS
GGATGATTGAAGTAAACCAC+CGG	0.649322	4:+16346673	None:intergenic
ATACGCGAAGATTGGCGAAG+CGG	0.653952	4:-16346525	MsG0480019218.01.T01:CDS
GCGGTTCACCGGTACCGAAG+GGG	0.656345	4:-16346750	MsG0480019218.01.T01:CDS
TTAAGACCCGAATAGCAGCT+GGG	0.660451	4:-16346552	MsG0480019218.01.T01:CDS
TCCGACCCGGTTGAGCAAAG+CGG	0.712572	4:-16346769	MsG0480019218.01.T01:CDS
CGACAAAGCTTCTATCATGG+GGG	0.730867	4:-16346867	MsG0480019218.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AAATTTGGTTATTTGCCGAT+AGG	-	Chr4:16346696-16346715	MsG0480019218.01.T01:CDS	30.0%
	AATTTGGTTATTTGCCGATA+GGG	-	Chr4:16346697-16346716	MsG0480019218.01.T01:CDS	30.0%
	TACTTCAATCATCCTTTGTT+TGG	-	Chr4:16346825-16346844	MsG0480019218.01.T01:CDS	30.0%
!	ACTTCAATCATCCTTTGTTT+GGG	-	Chr4:16346826-16346845	MsG0480019218.01.T01:CDS	30.0%
	AAAGAAGCTGAGAAAGAGTA+TGG	-	Chr4:16346858-16346877	MsG0480019218.01.T01:CDS	35.0%
	AAGTCTCTATGTTCAAAAGC+AGG	-	Chr4:16346666-16346685	MsG0480019218.01.T01:CDS	35.0%
	AGTCTCTATGTTCAAAAGCA+GGG	-	Chr4:16346667-16346686	MsG0480019218.01.T01:CDS	35.0%
!	AAGAAGCTGAGAAAGAGTAT+GGG	-	Chr4:16346859-16346878	MsG0480019218.01.T01:CDS	35.0%
!	CTTCAATCATCCTTTGTTTG+GGG	-	Chr4:16346827-16346846	MsG0480019218.01.T01:CDS	35.0%
	AATGGATATTCCGGCGATTT+CGG	-	Chr4:16346547-16346566	MsG0480019218.01.T01:CDS	40.0%
	ACGACAAAGCTTCTATCATG+GGG	-	Chr4:16346621-16346640	MsG0480019218.01.T01:CDS	40.0%
	AGCTAGGGAAGAAAGTGATT+CGG	-	Chr4:16346517-16346536	MsG0480019218.01.T01:CDS	40.0%
	GACGACAAAGCTTCTATCAT+GGG	-	Chr4:16346620-16346639	MsG0480019218.01.T01:CDS	40.0%
	GGATGATTGAAGTAAACCAC+CGG	+	Chr4:16346819-16346838	None:intergenic	40.0%
	GGGTCAGTAAATGGATATTC+CGG	-	Chr4:16346538-16346557	MsG0480019218.01.T01:CDS	40.0%
	GTCGTCAAATGATTCTTCTC+CGG	+	Chr4:16346606-16346625	None:intergenic	40.0%
	GTGGAATCACTATTCCTTGT+CGG	-	Chr4:16346895-16346914	MsG0480019218.01.T01:CDS	40.0%
	TCTATGTTCAAAAGCAGGGT+CGG	-	Chr4:16346671-16346690	MsG0480019218.01.T01:CDS	40.0%
	TGACGACAAAGCTTCTATCA+TGG	-	Chr4:16346619-16346638	MsG0480019218.01.T01:CDS	40.0%
	TGGAATCACTATTCCTTGTC+GGG	-	Chr4:16346896-16346915	MsG0480019218.01.T01:CDS	40.0%
	TTCAACAACTCCCCAAACAA+AGG	+	Chr4:16346840-16346859	None:intergenic	40.0%
!	AAGTGATTCGGGTCAGTAAA+TGG	-	Chr4:16346529-16346548	MsG0480019218.01.T01:CDS	40.0%
!!	GAGTATGGGTTTTGTCATCA+AGG	-	Chr4:16346873-16346892	MsG0480019218.01.T01:CDS	40.0%
!!	TATGGGTTTTGTCATCAAGG+TGG	-	Chr4:16346876-16346895	MsG0480019218.01.T01:CDS	40.0%
	AAAAAGACGGTGAGTTTCGG+CGG	-	Chr4:16346784-16346803	MsG0480019218.01.T01:CDS	45.0%
	AAAGCAGGGTCGGGTAAATT+TGG	-	Chr4:16346681-16346700	MsG0480019218.01.T01:CDS	45.0%
	CAAATGATTCTTCTCCGGTG+AGG	+	Chr4:16346601-16346620	None:intergenic	45.0%
	CGACAAAGCTTCTATCATGG+GGG	-	Chr4:16346622-16346641	MsG0480019218.01.T01:CDS	45.0%
	CTATGTTCAAAAGCAGGGTC+GGG	-	Chr4:16346672-16346691	MsG0480019218.01.T01:CDS	45.0%
	GCTAGGGAAGAAAGTGATTC+GGG	-	Chr4:16346518-16346537	MsG0480019218.01.T01:CDS	45.0%
	GGCAAAAAGACGGTGAGTTT+CGG	-	Chr4:16346781-16346800	MsG0480019218.01.T01:CDS	45.0%
	GGTCTTAACCCGTTCAAACT+CGG	+	Chr4:16346925-16346944	None:intergenic	45.0%
	GTGTATGTGGGGCAAAAAGA+CGG	-	Chr4:16346771-16346790	MsG0480019218.01.T01:CDS	45.0%
	TTAAGACCCGAATAGCAGCT+GGG	-	Chr4:16346937-16346956	MsG0480019218.01.T01:CDS	45.0%
	AAAAGACGGTGAGTTTCGGC+GGG	-	Chr4:16346785-16346804	MsG0480019218.01.T01:CDS	50.0%
	ATACGCGAAGATTGGCGAAG+CGG	-	Chr4:16346964-16346983	MsG0480019218.01.T01:CDS	50.0%
	CGAACAAGATACCGTCGTCT+CGG	-	Chr4:16346573-16346592	MsG0480019218.01.T01:CDS	50.0%
	CGAGACGACGGTATCTTGTT+CGG	+	Chr4:16346575-16346594	None:intergenic	50.0%
	GAGACGACGGTATCTTGTTC+GGG	+	Chr4:16346574-16346593	None:intergenic	50.0%
	GGCATTTAGCGGTGTATGTG+GGG	-	Chr4:16346760-16346779	MsG0480019218.01.T01:CDS	50.0%
	GGGCATTTAGCGGTGTATGT+GGG	-	Chr4:16346759-16346778	MsG0480019218.01.T01:CDS	50.0%
	GTCGGGTTACCGAGTTTGAA+CGG	-	Chr4:16346913-16346932	MsG0480019218.01.T01:CDS	50.0%
	GTTAAGACCCGAATAGCAGC+TGG	-	Chr4:16346936-16346955	MsG0480019218.01.T01:CDS	50.0%
	TACACCGCTAAATGCCCCTT+CGG	+	Chr4:16346756-16346775	None:intergenic	50.0%
	TATCCGACCCAGCTGCTATT+CGG	+	Chr4:16346947-16346966	None:intergenic	50.0%
	TCAAACTCGGTAACCCGACA+AGG	+	Chr4:16346912-16346931	None:intergenic	50.0%
	TCGGGTTACCGAGTTTGAAC+GGG	-	Chr4:16346914-16346933	MsG0480019218.01.T01:CDS	50.0%
	ATCCGACCCAGCTGCTATTC+GGG	+	Chr4:16346946-16346965	None:intergenic	55.0%
	GCTAAATGCCCCTTCGGTAC+CGG	+	Chr4:16346750-16346769	None:intergenic	55.0%
	GGTGAACCGCTTTGCTCAAC+CGG	+	Chr4:16346729-16346748	None:intergenic	55.0%
	GGTTGAGCAAAGCGGTTCAC+CGG	-	Chr4:16346728-16346747	MsG0480019218.01.T01:CDS	55.0%
	GTGAACCGCTTTGCTCAACC+GGG	+	Chr4:16346728-16346747	None:intergenic	55.0%
	TGGGTCGGATACGCGAAGAT+TGG	-	Chr4:16346956-16346975	MsG0480019218.01.T01:CDS	55.0%
	TTGTTCGGGTCCGAAATCGC+CGG	+	Chr4:16346560-16346579	None:intergenic	55.0%
!	GGGGCATTTAGCGGTGTATG+TGG	-	Chr4:16346758-16346777	MsG0480019218.01.T01:CDS	55.0%
!!	GGTACCGAAGGGGCATTTAG+CGG	-	Chr4:16346749-16346768	MsG0480019218.01.T01:CDS	55.0%
	ACCGCTTTGCTCAACCGGGT+CGG	+	Chr4:16346724-16346743	None:intergenic	60.0%
	GACCCGAATAGCAGCTGGGT+CGG	-	Chr4:16346941-16346960	MsG0480019218.01.T01:CDS	60.0%
	TCAACCGGGTCGGACCCTAT+CGG	+	Chr4:16346714-16346733	None:intergenic	60.0%
	TCCGACCCGGTTGAGCAAAG+CGG	-	Chr4:16346720-16346739	MsG0480019218.01.T01:CDS	60.0%
	TTTGCCGATAGGGTCCGACC+CGG	-	Chr4:16346707-16346726	MsG0480019218.01.T01:CDS	60.0%
!	AAGCGGTTCACCGGTACCGA+AGG	-	Chr4:16346737-16346756	MsG0480019218.01.T01:CDS	60.0%
!	AGCGGTTCACCGGTACCGAA+GGG	-	Chr4:16346738-16346757	MsG0480019218.01.T01:CDS	60.0%
!!	CGGTGAGTTTCGGCGGGTTT+TGG	-	Chr4:16346791-16346810	MsG0480019218.01.T01:CDS	60.0%
!!	GTTTCGGCGGGTTTTGGTGC+CGG	-	Chr4:16346797-16346816	MsG0480019218.01.T01:CDS	60.0%
!	GCGGTTCACCGGTACCGAAG+GGG	-	Chr4:16346739-16346758	MsG0480019218.01.T01:CDS	65.0%
!!!	TCGGCGGGTTTTGGTGCCGG+TGG	-	Chr4:16346800-16346819	MsG0480019218.01.T01:CDS	70.0%
	CCGTCGTCTCGGCGCCTCAC+CGG	-	Chr4:16346584-16346603	MsG0480019218.01.T01:CDS	75.0%
!!	CCGGTGAGGCGCCGAGACGA+CGG	+	Chr4:16346587-16346606	None:intergenic	75.0%

Chromosome	Type	Strat	End	Strand	Name
Chr4	gene	16346507	16347004	16346507	ID=MsG0480019218.01;Name=MsG0480019218.01
Chr4	mRNA	16346507	16347004	16346507	ID=MsG0480019218.01.T01;Parent=MsG0480019218.01;Name=MsG0480019218.01.T01;_AED=0.49;_eAED=0.49;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|165
Chr4	exon	16346507	16347004	16346507	ID=MsG0480019218.01.T01:exon:4348;Parent=MsG0480019218.01.T01
Chr4	CDS	16346507	16347004	16346507	ID=MsG0480019218.01.T01:cds;Parent=MsG0480019218.01.T01

Gene Sequence

>MsG0480019218.01.T01

ATGCAATTCAAGCTAGGGAAGAAAGTGATTCGGGTCAGTAAATGGATATTCCGGCGATTTCGGACCCGAACAAGATACCGTCGTCTCGGCGCCTCACCGGAGAAGAATCATTTGACGACAAAGCTTCTATCATGGGGGAATAAGTTAACTACAAAAGCAAAGTCTCTATGTTCAAAAGCAGGGTCGGGTAAATTTGGTTATTTGCCGATAGGGTCCGACCCGGTTGAGCAAAGCGGTTCACCGGTACCGAAGGGGCATTTAGCGGTGTATGTGGGGCAAAAAGACGGTGAGTTTCGGCGGGTTTTGGTGCCGGTGGTTTACTTCAATCATCCTTTGTTTGGGGAGTTGTTGAAAGAAGCTGAGAAAGAGTATGGGTTTTGTCATCAAGGTGGAATCACTATTCCTTGTCGGGTTACCGAGTTTGAACGGGTTAAGACCCGAATAGCAGCTGGGTCGGATACGCGAAGATTGGCGAAGCGGAGACATTTTGATTGCTGA

Protein sequence

>MsG0480019218.01.T01

MQFKLGKKVIRVSKWIFRRFRTRTRYRRLGASPEKNHLTTKLLSWGNKLTTKAKSLCSKAGSGKFGYLPIGSDPVEQSGSPVPKGHLAVYVGQKDGEFRRVLVPVVYFNHPLFGELLKEAEKEYGFCHQGGITIPCRVTEFERVKTRIAAGSDTRRLAKRRHFDC*