AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480019533.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480019533.01.T01 MTR_4g033015 92.244 361 28 0 5 365 3 363 0.0 664
MsG0480019533.01.T01 MTR_4g033200 91.967 361 29 0 5 365 3 363 0.0 663
MsG0480019533.01.T01 MTR_4g033150 89.589 365 36 1 1 365 1 363 0.0 652
MsG0480019533.01.T01 MTR_4g088200 72.603 365 96 2 1 365 1 361 0.0 554
MsG0480019533.01.T01 MTR_4g033170 75.342 365 82 2 1 365 1 357 0.0 534
MsG0480019533.01.T01 MTR_4g088190 74.863 366 89 2 1 365 1 364 0.0 533
MsG0480019533.01.T01 MTR_4g033085 75.068 365 83 2 1 365 1 357 0.0 510
MsG0480019533.01.T01 MTR_0794s0020 88.446 251 27 1 1 251 1 249 3.60e-164 459
MsG0480019533.01.T01 MTR_5g074680 51.515 363 165 6 7 364 3 359 2.98e-125 365
MsG0480019533.01.T01 MTR_7g014570 51.275 353 161 4 13 365 11 352 1.56e-124 362
MsG0480019533.01.T01 MTR_5g074600 50.847 354 166 5 14 365 12 359 3.60e-124 362
MsG0480019533.01.T01 MTR_5g074580 49.725 364 172 6 7 365 3 360 3.66e-119 349
MsG0480019533.01.T01 MTR_4g088345 51.130 354 164 4 13 365 11 356 3.25e-117 344
MsG0480019533.01.T01 MTR_7g014510 47.159 352 180 4 14 365 12 357 7.19e-117 343
MsG0480019533.01.T01 MTR_7g024500 48.148 351 170 4 13 363 10 348 2.01e-115 339
MsG0480019533.01.T01 MTR_1g076940 47.945 365 181 7 4 365 3 361 7.61e-115 338
MsG0480019533.01.T01 MTR_5g074610 48.459 357 151 3 9 365 3 326 2.42e-114 335
MsG0480019533.01.T01 MTR_7g024520 49.003 351 167 4 13 363 10 348 2.15e-112 332
MsG0480019533.01.T01 MTR_4g079160 47.309 353 177 3 14 365 11 355 2.00e-110 327
MsG0480019533.01.T01 MTR_5g074540 46.479 355 164 6 13 365 11 341 6.04e-107 317
MsG0480019533.01.T01 MTR_4g088340 49.560 341 163 4 26 365 1 333 7.66e-107 317
MsG0480019533.01.T01 MTR_3g083620 37.609 343 199 9 22 361 27 357 6.34e-64 207
MsG0480019533.01.T01 MTR_4g038440 35.635 362 219 8 8 365 17 368 5.12e-63 206
MsG0480019533.01.T01 MTR_0017s0100 35.635 362 219 8 8 365 17 368 1.52e-61 202
MsG0480019533.01.T01 MTR_0079s0070 35.965 342 205 8 28 365 3 334 2.62e-59 195
MsG0480019533.01.T01 MTR_1g076930 43.852 244 126 6 26 265 1 237 9.70e-59 191
MsG0480019533.01.T01 MTR_1g036460 30.726 358 230 8 2 352 9 355 2.02e-47 165
MsG0480019533.01.T01 MTR_7g012090 32.845 341 208 10 34 363 40 370 2.69e-47 165
MsG0480019533.01.T01 MTR_7g011990 31.195 343 208 10 34 364 40 366 1.01e-46 163
MsG0480019533.01.T01 MTR_7g011900 32.249 338 214 9 34 364 40 369 2.20e-46 162
MsG0480019533.01.T01 MTR_7g012070 32.258 341 210 11 34 363 40 370 3.49e-46 162
MsG0480019533.01.T01 MTR_8g068870 33.526 346 214 10 20 357 19 356 3.76e-46 161
MsG0480019533.01.T01 MTR_1g036490 31.099 373 235 11 2 364 9 369 5.05e-46 161
MsG0480019533.01.T01 MTR_1g096310 30.447 358 231 8 2 352 12 358 2.47e-45 159
MsG0480019533.01.T01 MTR_7g012040 32.335 334 213 8 34 363 39 363 2.04e-44 157
MsG0480019533.01.T01 MTR_5g098170 34.731 334 197 13 33 357 33 354 2.65e-44 157
MsG0480019533.01.T01 MTR_2g055940 31.940 335 213 9 34 363 34 358 3.23e-43 154
MsG0480019533.01.T01 MTR_7g118320 33.333 330 205 10 28 350 26 347 6.99e-43 153
MsG0480019533.01.T01 MTR_8g068690 33.538 325 203 8 28 346 40 357 1.38e-42 152
MsG0480019533.01.T01 MTR_8g068750 32.641 337 213 9 28 357 41 370 2.01e-42 152
MsG0480019533.01.T01 MTR_7g012020 32.239 335 213 8 34 363 39 364 3.55e-42 151
MsG0480019533.01.T01 MTR_0591s0010 33.538 325 203 8 28 346 41 358 6.38e-42 150
MsG0480019533.01.T01 MTR_3g092900 31.897 348 219 9 9 351 15 349 4.24e-41 148
MsG0480019533.01.T01 MTR_7g118300 32.344 337 204 9 28 355 42 363 1.88e-40 147
MsG0480019533.01.T01 MTR_1g036510 30.330 333 218 7 27 352 44 369 4.91e-40 145
MsG0480019533.01.T01 MTR_3g021440 32.722 327 208 8 30 352 38 356 6.49e-39 142
MsG0480019533.01.T01 MTR_2g072790 32.047 337 210 9 33 362 27 351 7.85e-39 142
MsG0480019533.01.T01 MTR_8g024160 34.510 255 158 5 100 352 68 315 1.25e-36 135
MsG0480019533.01.T01 MTR_3g021430 29.944 354 229 10 6 352 2 343 1.79e-36 135
MsG0480019533.01.T01 MTR_3g096050 30.747 348 219 11 25 364 24 357 6.69e-36 134
MsG0480019533.01.T01 MTR_4g038440 33.333 261 164 5 8 265 17 270 7.50e-36 132
MsG0480019533.01.T01 MTR_8g068570 31.833 311 172 9 28 332 41 317 3.80e-35 131
MsG0480019533.01.T01 MTR_8g068730 30.473 338 187 10 28 357 41 338 9.10e-33 125
MsG0480019533.01.T01 MTR_3g096070 29.565 345 200 12 25 362 24 332 6.03e-32 123
MsG0480019533.01.T01 MTR_6g078850 28.161 348 230 9 11 350 33 368 9.12e-31 122
MsG0480019533.01.T01 MTR_7g012020 30.705 241 158 6 34 269 39 275 2.24e-27 110
MsG0480019533.01.T01 MTR_1g050220 34.416 154 88 2 196 349 147 287 1.13e-22 97.1
MsG0480019533.01.T01 MTR_8g068690 29.268 246 165 6 28 268 40 281 2.88e-21 92.8
MsG0480019533.01.T01 MTR_1g036510 29.268 246 163 6 27 265 44 285 4.44e-21 92.4
MsG0480019533.01.T01 MTR_3g096050 31.579 247 152 9 25 265 24 259 2.20e-20 90.1
MsG0480019533.01.T01 MTR_8g068740 28.727 275 151 8 28 294 41 278 3.69e-20 89.7
MsG0480019533.01.T01 MTR_7g012020 30.476 210 137 6 34 238 39 244 1.00e-18 85.1
MsG0480019533.01.T01 MTR_8g068600 28.017 232 158 6 40 266 27 254 2.01e-17 82.0
MsG0480019533.01.T01 MTR_8g068620 35.965 114 71 1 155 268 2 113 8.34e-16 73.2
MsG0480019533.01.T01 MTR_4g025570 41.414 99 51 3 12 110 17 108 5.01e-14 70.9
MsG0480019533.01.T01 MTR_4g025570 42.708 96 51 3 267 361 122 214 6.08e-14 70.9
MsG0480019533.01.T01 MTR_1g036510 27.397 219 148 6 27 238 44 258 4.99e-13 68.9
MsG0480019533.01.T01 MTR_8g068760 41.053 95 51 3 265 357 135 226 9.90e-13 67.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480019533.01.T01 AT4G35160 33.973 365 210 12 18 364 29 380 3.46e-49 170
MsG0480019533.01.T01 AT4G35150 30.726 358 188 10 18 364 15 323 2.97e-48 166
MsG0480019533.01.T01 AT5G54160 33.127 323 205 7 33 352 34 348 2.79e-44 156
MsG0480019533.01.T01 AT1G51990 31.367 373 229 12 4 365 6 362 1.90e-43 154
MsG0480019533.01.T01 AT1G77520 31.217 378 223 12 9 364 18 380 1.40e-42 152
MsG0480019533.01.T01 AT1G51990 31.383 376 225 13 4 365 6 362 1.06e-41 150
MsG0480019533.01.T01 AT1G33030 31.325 332 209 10 33 355 21 342 2.13e-39 143
MsG0480019533.01.T01 AT3G53140 31.351 370 225 13 6 362 3 356 2.29e-39 143
MsG0480019533.01.T01 AT5G53810 30.259 347 222 9 31 364 38 377 4.92e-35 132
MsG0480019533.01.T01 AT1G77530 32.353 374 224 14 9 364 18 380 5.58e-35 132
MsG0480019533.01.T01 AT1G63140 29.730 370 236 11 11 364 19 380 5.76e-34 129
MsG0480019533.01.T01 AT1G63140 29.730 370 236 11 11 364 39 400 9.19e-34 129
MsG0480019533.01.T01 AT1G76790 30.163 368 215 15 8 357 8 351 2.05e-27 111
MsG0480019533.01.T01 AT1G62900 35.938 192 118 3 175 364 16 204 1.95e-25 102
MsG0480019533.01.T01 AT1G21130 28.402 338 207 13 33 352 40 360 5.99e-25 104
MsG0480019533.01.T01 AT1G21100 27.976 336 211 12 33 352 40 360 3.11e-24 102
MsG0480019533.01.T01 AT1G21110 27.492 331 209 12 33 347 40 355 1.37e-23 100
MsG0480019533.01.T01 AT5G37170 31.443 194 127 4 174 364 43 233 3.70e-23 97.1
MsG0480019533.01.T01 AT5G37170 31.443 194 127 4 174 364 143 333 6.67e-23 98.2
MsG0480019533.01.T01 AT1G21120 27.083 336 214 12 33 352 40 360 1.36e-22 98.2
MsG0480019533.01.T01 AT1G21120 27.083 336 214 12 33 352 88 408 2.94e-22 97.8
MsG0480019533.01.T01 AT5G37170 34.104 173 105 5 196 362 14 183 8.51e-22 92.0
MsG0480019533.01.T01 AT1G33030 30.000 240 153 9 33 265 21 252 6.02e-18 83.2
MsG0480019533.01.T01 AT1G33030 30.000 240 153 9 33 265 21 252 1.01e-17 82.8
MsG0480019533.01.T01 AT1G63140 27.509 269 176 8 11 265 39 302 5.49e-16 78.2

Find 61 sgRNAs with CRISPR-Local

Find 94 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCACAAGAGGCGAATCATTT+TGG 0.147947 4:+21743865 MsG0480019533.01.T01:CDS
ATGATGATGACTTTGTTAAA+TGG 0.247172 4:+21744831 MsG0480019533.01.T01:CDS
TAAAACAAATGAGTTGAAAT+TGG 0.270517 4:+21744797 MsG0480019533.01.T01:CDS
TGTATATATGTTTGTATAGA+TGG 0.289789 4:-21743464 None:intergenic
TTCTTCACTGCTAGTGCTAT+TGG 0.295654 4:-21743418 None:intergenic
CTCAGTGAGCCATTTCTTAG+AGG 0.316815 4:-21743817 None:intergenic
TAGCCATGTGTTTGAAGGTT+TGG 0.322024 4:+21743986 MsG0480019533.01.T01:CDS
TACAGTGGATTCTGCATGAT+TGG 0.329305 4:+21744658 MsG0480019533.01.T01:intron
TGAATTGTCCTTGAAGATAT+TGG 0.335230 4:+21744686 MsG0480019533.01.T01:CDS
TACAGGACACTAACTTTAGA+AGG 0.365683 4:-21743603 None:intergenic
GCTAATTCAGTAAGAGTCAT+TGG 0.368956 4:-21743561 None:intergenic
AAGAAAGAGAGAAGAAAGAA+TGG 0.382447 4:+21744856 MsG0480019533.01.T01:CDS
GATAGACTTGAAGCCGCATA+TGG 0.398440 4:-21744925 None:intergenic
AACGAAAATTTGAAATTTGT+TGG 0.401585 4:+21744128 MsG0480019533.01.T01:CDS
TGTTTGTCACCATTTGTTAC+CGG 0.405515 4:+21743759 MsG0480019533.01.T01:CDS
GCAGTTCTCCAATATCTTCA+AGG 0.406503 4:-21744694 None:intergenic
TTAGGTGAGGAAAAGTTTCA+TGG 0.421372 4:-21744058 None:intergenic
GAGTATAGCCATGTGTTTGA+AGG 0.421443 4:+21743981 MsG0480019533.01.T01:CDS
GTTTGAAGGTTTGGAGTCTC+TGG 0.426025 4:+21743995 MsG0480019533.01.T01:CDS
CCACTGATGAGAAGCATTGA+AGG 0.442792 4:-21743726 None:intergenic
TGTGGTACTCCTCTAAGAAA+TGG 0.454917 4:+21743808 MsG0480019533.01.T01:CDS
GTTGATGTTGGAGGTGGCAC+AGG 0.461040 4:+21744017 MsG0480019533.01.T01:CDS
ATTTCAGGAAAAGGGACAAA+AGG 0.472680 4:+21744726 MsG0480019533.01.T01:CDS
AGAAAGAGAGAAGAAAGAAT+GGG 0.494469 4:+21744857 MsG0480019533.01.T01:CDS
TTGGAGTCTCTGGTTGATGT+TGG 0.495110 4:+21744005 MsG0480019533.01.T01:CDS
GAAAATTTGAAATTTGTTGG+TGG 0.495217 4:+21744131 MsG0480019533.01.T01:CDS
GAGTCTCTGGTTGATGTTGG+AGG 0.503583 4:+21744008 MsG0480019533.01.T01:CDS
ACACTGATGAATGGCAAAGA+AGG 0.516027 4:+21743672 MsG0480019533.01.T01:CDS
GATGTTGGAGGTGGCACAGG+AGG 0.520874 4:+21744020 MsG0480019533.01.T01:CDS
CTCAAATCAGCCGTGGAGCT+AGG 0.521896 4:+21743507 MsG0480019533.01.T01:CDS
TGAAGCGATCCGGTAACAAA+TGG 0.526003 4:-21743768 None:intergenic
CACGGCTGATTTGAGTGCCA+TGG 0.545739 4:-21743499 None:intergenic
ATACAGCTTTATAAATTCCA+TGG 0.553200 4:+21743482 MsG0480019533.01.T01:CDS
ACGACATGGATGAAGCGATC+CGG 0.554100 4:-21743778 None:intergenic
TTGAGTAAAACAGCATCTGC+AGG 0.557051 4:-21744176 None:intergenic
GCAAGCGCAAGAGACGATAC+AGG 0.558531 4:-21743620 None:intergenic
AGTCTCTTTGAGAGCACAAG+AGG 0.560449 4:+21743852 MsG0480019533.01.T01:CDS
GAGTATGTTTCAAGAGGCCA+TGG 0.566423 4:+21743923 MsG0480019533.01.T01:CDS
ATCTGCTATGCCTAGCTCCA+CGG 0.571981 4:-21743517 None:intergenic
CCTTCAATGCTTCTCATCAG+TGG 0.573536 4:+21743726 MsG0480019533.01.T01:CDS
GATTTGAGAATCAGCTGCCA+TGG 0.584730 4:-21743940 None:intergenic
TCTCTGGTTGATGTTGGAGG+TGG 0.586831 4:+21744011 MsG0480019533.01.T01:CDS
TACCACATGTTGAGACGACA+TGG 0.595315 4:-21743792 None:intergenic
ATCCATGTCGTCTCAACATG+TGG 0.596571 4:+21743790 MsG0480019533.01.T01:CDS
CATAGTATTTGACCAACCAC+AGG 0.602779 4:+21744085 MsG0480019533.01.T01:CDS
ATAGACTTGAAGCCGCATAT+GGG 0.603518 4:-21744924 None:intergenic
TTGCGCTTGCTAACACACAA+TGG 0.607728 4:+21743633 MsG0480019533.01.T01:CDS
GGACACTAACTTTAGAAGGG+TGG 0.621869 4:-21743599 None:intergenic
GAAATGGCTCACTGAGGACA+AGG 0.624424 4:+21743824 MsG0480019533.01.T01:CDS
AGTAAAACAGCATCTGCAGG+AGG 0.624714 4:-21744173 None:intergenic
TGAACTGAGTATGTTTCAAG+AGG 0.628302 4:+21743917 MsG0480019533.01.T01:CDS
AGGAAAAGGGACAAAAGGAA+AGG 0.628316 4:+21744731 MsG0480019533.01.T01:CDS
CATGGCACTCAAATCAGCCG+TGG 0.637083 4:+21743500 MsG0480019533.01.T01:CDS
GAGAAACTCATATTTGACGC+AGG 0.638948 4:+21744879 MsG0480019533.01.T01:CDS
GCAGATGCAATCCACAACCA+TGG 0.642290 4:+21743534 MsG0480019533.01.T01:CDS
ACAGGACACTAACTTTAGAA+GGG 0.644029 4:-21743602 None:intergenic
CTGATGAGAAGCATTGAAGG+AGG 0.666811 4:-21743723 None:intergenic
TATAAGATTACACCCATATG+CGG 0.675606 4:+21744912 MsG0480019533.01.T01:CDS
ACTCCAAACCTTCAAACACA+TGG 0.677292 4:-21743989 None:intergenic
GTAAAACAGCATCTGCAGGA+GGG 0.681270 4:-21744172 None:intergenic
CTCTAAGAAATGGCTCACTG+AGG 0.732533 4:+21743818 MsG0480019533.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AGTTAAAGATATATTTTTTT+GGG + Chr4:21744222-21744241 MsG0480019533.01.T01:intron 10.0%
!!! GAGTTAAAGATATATTTTTT+TGG + Chr4:21744221-21744240 MsG0480019533.01.T01:intron 15.0%
!!! GATAAAATATTTTTTTACAG+TGG + Chr4:21744643-21744662 MsG0480019533.01.T01:intron 15.0%
!! CTAATTAATCTTTGCAATAA+AGG + Chr4:21744549-21744568 MsG0480019533.01.T01:intron 20.0%
!! TGTATATATGTTTGTATAGA+TGG - Chr4:21743467-21743486 None:intergenic 20.0%
!! TGTTCATTATCTTATATCAA+CGG - Chr4:21744460-21744479 None:intergenic 20.0%
!!! AACGAAAATTTGAAATTTGT+TGG + Chr4:21744128-21744147 MsG0480019533.01.T01:CDS 20.0%
!!! GAGTAATTTTCTTATGTTAT+TGG + Chr4:21744496-21744515 MsG0480019533.01.T01:intron 20.0%
!!! TAAAACAAATGAGTTGAAAT+TGG + Chr4:21744797-21744816 MsG0480019533.01.T01:CDS 20.0%
! TAATTAGTCATCAACTAAAC+TGG - Chr4:21744536-21744555 None:intergenic 25.0%
!! AAAAAAGCTATTTCAGGAAA+AGG + Chr4:21744717-21744736 MsG0480019533.01.T01:CDS 25.0%
!! AAAAAGCTATTTCAGGAAAA+GGG + Chr4:21744718-21744737 MsG0480019533.01.T01:CDS 25.0%
!! ATACAGCTTTATAAATTCCA+TGG + Chr4:21743482-21743501 MsG0480019533.01.T01:CDS 25.0%
!! ATGATGATGACTTTGTTAAA+TGG + Chr4:21744831-21744850 MsG0480019533.01.T01:CDS 25.0%
!!! GAAAATTTGAAATTTGTTGG+TGG + Chr4:21744131-21744150 MsG0480019533.01.T01:CDS 25.0%
AAGAAAGAGAGAAGAAAGAA+TGG + Chr4:21744856-21744875 MsG0480019533.01.T01:CDS 30.0%
ACCGATCTTAAAATTGTCAA+CGG + Chr4:21744393-21744412 MsG0480019533.01.T01:intron 30.0%
AGAAAGAGAGAAGAAAGAAT+GGG + Chr4:21744857-21744876 MsG0480019533.01.T01:CDS 30.0%
TATAAGATTACACCCATATG+CGG + Chr4:21744912-21744931 MsG0480019533.01.T01:CDS 30.0%
TGAATTGTCCTTGAAGATAT+TGG + Chr4:21744686-21744705 MsG0480019533.01.T01:CDS 30.0%
! AACTGCAAAAAAGCTATTTC+AGG + Chr4:21744711-21744730 MsG0480019533.01.T01:CDS 30.0%
! GGTCAAATACTATGCATTTT+AGG - Chr4:21744079-21744098 None:intergenic 30.0%
!! AATACTATGCATTTTAGGTG+AGG - Chr4:21744074-21744093 None:intergenic 30.0%
ATTTCAGGAAAAGGGACAAA+AGG + Chr4:21744726-21744745 MsG0480019533.01.T01:CDS 35.0%
CAGGTTGTAAAAAACTTGTC+TGG + Chr4:21744104-21744123 MsG0480019533.01.T01:CDS 35.0%
GCAAAAACAACACTGATGAA+TGG + Chr4:21743663-21743682 MsG0480019533.01.T01:CDS 35.0%
GCTAATTCAGTAAGAGTCAT+TGG - Chr4:21743564-21743583 None:intergenic 35.0%
TACAGGACACTAACTTTAGA+AGG - Chr4:21743606-21743625 None:intergenic 35.0%
TGAACTGAGTATGTTTCAAG+AGG + Chr4:21743917-21743936 MsG0480019533.01.T01:CDS 35.0%
TTAGGTGAGGAAAAGTTTCA+TGG - Chr4:21744061-21744080 None:intergenic 35.0%
! ACAGGACACTAACTTTAGAA+GGG - Chr4:21743605-21743624 None:intergenic 35.0%
! AGGGAACAAGCAATTTTACA+AGG - Chr4:21744276-21744295 None:intergenic 35.0%
! GGGAACAAGCAATTTTACAA+GGG - Chr4:21744275-21744294 None:intergenic 35.0%
! TGTTTGTCACCATTTGTTAC+CGG + Chr4:21743759-21743778 MsG0480019533.01.T01:CDS 35.0%
!!! AGTTTTTTACAACCTGTGGT+TGG - Chr4:21744100-21744119 None:intergenic 35.0%
!!! GACAAGTTTTTTACAACCTG+TGG - Chr4:21744104-21744123 None:intergenic 35.0%
!!! GCCGTTGACAATTTTAAGAT+CGG - Chr4:21744397-21744416 None:intergenic 35.0%
!!! TAAGAGTCATTGGTTTTCCA+TGG - Chr4:21743554-21743573 None:intergenic 35.0%
ACACTGATGAATGGCAAAGA+AGG + Chr4:21743672-21743691 MsG0480019533.01.T01:CDS 40.0%
ACTCCAAACCTTCAAACACA+TGG - Chr4:21743992-21744011 None:intergenic 40.0%
AGGAAAAGGGACAAAAGGAA+AGG + Chr4:21744731-21744750 MsG0480019533.01.T01:CDS 40.0%
AGTTAACTGCCGCTGATATA+AGG - Chr4:21744296-21744315 None:intergenic 40.0%
ATAGACTTGAAGCCGCATAT+GGG - Chr4:21744927-21744946 None:intergenic 40.0%
CATAGTATTTGACCAACCAC+AGG + Chr4:21744085-21744104 MsG0480019533.01.T01:CDS 40.0%
GAGAAACTCATATTTGACGC+AGG + Chr4:21744879-21744898 MsG0480019533.01.T01:CDS 40.0%
GAGTATAGCCATGTGTTTGA+AGG + Chr4:21743981-21744000 MsG0480019533.01.T01:CDS 40.0%
GCAGTTCTCCAATATCTTCA+AGG - Chr4:21744697-21744716 None:intergenic 40.0%
GTTAACTGCCGCTGATATAA+GGG - Chr4:21744295-21744314 None:intergenic 40.0%
TAGCCATGTGTTTGAAGGTT+TGG + Chr4:21743986-21744005 MsG0480019533.01.T01:CDS 40.0%
TGCTTGTTCCCTTATATCAG+CGG + Chr4:21744284-21744303 MsG0480019533.01.T01:intron 40.0%
TTCTTCACTGCTAGTGCTAT+TGG - Chr4:21743421-21743440 None:intergenic 40.0%
TTGAGTAAAACAGCATCTGC+AGG - Chr4:21744179-21744198 None:intergenic 40.0%
! CACAAGAGGCGAATCATTTT+GGG + Chr4:21743866-21743885 MsG0480019533.01.T01:CDS 40.0%
! TACAGTGGATTCTGCATGAT+TGG + Chr4:21744658-21744677 MsG0480019533.01.T01:intron 40.0%
! TGTGGTACTCCTCTAAGAAA+TGG + Chr4:21743808-21743827 MsG0480019533.01.T01:CDS 40.0%
!! TTCGATCATAAAAGCGTCAG+TGG - Chr4:21744349-21744368 None:intergenic 40.0%
!!! TCATTGGTTTTCCATGGTTG+TGG - Chr4:21743548-21743567 None:intergenic 40.0%
!!! TGCAGATGCTGTTTTACTCA+AGG + Chr4:21744178-21744197 MsG0480019533.01.T01:CDS 40.0%
AGTAAAACAGCATCTGCAGG+AGG - Chr4:21744176-21744195 None:intergenic 45.0%
ATCCATGTCGTCTCAACATG+TGG + Chr4:21743790-21743809 MsG0480019533.01.T01:CDS 45.0%
CCTTCAATGCTTCTCATCAG+TGG + Chr4:21743726-21743745 MsG0480019533.01.T01:CDS 45.0%
CTCAGTGAGCCATTTCTTAG+AGG - Chr4:21743820-21743839 None:intergenic 45.0%
GAGTATGTTTCAAGAGGCCA+TGG + Chr4:21743923-21743942 MsG0480019533.01.T01:CDS 45.0%
GATAGACTTGAAGCCGCATA+TGG - Chr4:21744928-21744947 None:intergenic 45.0%
GATTTGAGAATCAGCTGCCA+TGG - Chr4:21743943-21743962 None:intergenic 45.0%
GTAAAACAGCATCTGCAGGA+GGG - Chr4:21744175-21744194 None:intergenic 45.0%
TACCACATGTTGAGACGACA+TGG - Chr4:21743795-21743814 None:intergenic 45.0%
TATCGCTGAAAAATGCCGAG+CGG - Chr4:21744320-21744339 None:intergenic 45.0%
TGAAGCGATCCGGTAACAAA+TGG - Chr4:21743771-21743790 None:intergenic 45.0%
! AGTCTCTTTGAGAGCACAAG+AGG + Chr4:21743852-21743871 MsG0480019533.01.T01:CDS 45.0%
! CTCTAAGAAATGGCTCACTG+AGG + Chr4:21743818-21743837 MsG0480019533.01.T01:CDS 45.0%
! GCACAAGAGGCGAATCATTT+TGG + Chr4:21743865-21743884 MsG0480019533.01.T01:CDS 45.0%
! GGACACTAACTTTAGAAGGG+TGG - Chr4:21743602-21743621 None:intergenic 45.0%
! GTTTGAAGGTTTGGAGTCTC+TGG + Chr4:21743995-21744014 MsG0480019533.01.T01:CDS 45.0%
! TTGCGCTTGCTAACACACAA+TGG + Chr4:21743633-21743652 MsG0480019533.01.T01:CDS 45.0%
! TTGGAGTCTCTGGTTGATGT+TGG + Chr4:21744005-21744024 MsG0480019533.01.T01:CDS 45.0%
!! CCACTGATGAGAAGCATTGA+AGG - Chr4:21743729-21743748 None:intergenic 45.0%
!! CTGATGAGAAGCATTGAAGG+AGG - Chr4:21743726-21743745 None:intergenic 45.0%
ACGACATGGATGAAGCGATC+CGG - Chr4:21743781-21743800 None:intergenic 50.0%
ATCTGCTATGCCTAGCTCCA+CGG - Chr4:21743520-21743539 None:intergenic 50.0%
GAAATGGCTCACTGAGGACA+AGG + Chr4:21743824-21743843 MsG0480019533.01.T01:CDS 50.0%
GCAGATGCAATCCACAACCA+TGG + Chr4:21743534-21743553 MsG0480019533.01.T01:CDS 50.0%
TGCCACTGAAAAATGCCGAG+CGG + Chr4:21744424-21744443 MsG0480019533.01.T01:intron 50.0%
! GAGTCTCTGGTTGATGTTGG+AGG + Chr4:21744008-21744027 MsG0480019533.01.T01:CDS 50.0%
! TCTCTGGTTGATGTTGGAGG+TGG + Chr4:21744011-21744030 MsG0480019533.01.T01:CDS 50.0%
AACGGCAGTTAACTGCCGCT+CGG - Chr4:21744442-21744461 None:intergenic 55.0%
AGCGGCAGTTAACTACCGCT+CGG + Chr4:21744302-21744321 MsG0480019533.01.T01:intron 55.0%
CATGGCACTCAAATCAGCCG+TGG + Chr4:21743500-21743519 MsG0480019533.01.T01:CDS 55.0%
CTCAAATCAGCCGTGGAGCT+AGG + Chr4:21743507-21743526 MsG0480019533.01.T01:CDS 55.0%
GCAAGCGCAAGAGACGATAC+AGG - Chr4:21743623-21743642 None:intergenic 55.0%
GTTGATGTTGGAGGTGGCAC+AGG + Chr4:21744017-21744036 MsG0480019533.01.T01:CDS 55.0%
!! CACGGCTGATTTGAGTGCCA+TGG - Chr4:21743502-21743521 None:intergenic 55.0%
!! TGCCGCTCGGCATTTTTCAG+TGG - Chr4:21744429-21744448 None:intergenic 55.0%
GATGTTGGAGGTGGCACAGG+AGG + Chr4:21744020-21744039 MsG0480019533.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr4 gene 21743405 21744965 21743405 ID=MsG0480019533.01;Name=MsG0480019533.01
Chr4 mRNA 21743405 21744965 21743405 ID=MsG0480019533.01.T01;Parent=MsG0480019533.01;Name=MsG0480019533.01.T01;_AED=0.21;_eAED=0.22;_QI=0|0|0|1|1|1|2|0|365
Chr4 exon 21743405 21744199 21743405 ID=MsG0480019533.01.T01:exon:8266;Parent=MsG0480019533.01.T01
Chr4 exon 21744663 21744965 21744663 ID=MsG0480019533.01.T01:exon:8267;Parent=MsG0480019533.01.T01
Chr4 CDS 21743405 21744199 21743405 ID=MsG0480019533.01.T01:cds;Parent=MsG0480019533.01.T01
Chr4 CDS 21744663 21744965 21744663 ID=MsG0480019533.01.T01:cds;Parent=MsG0480019533.01.T01
Gene Sequence

>MsG0480019533.01.T01

ATGAATTTCAGTACCAATAGCACTAGCAGTGAAGAAAATCAGCTGTATCATGCTCAAATCCATCTATACAAACATATATACAGCTTTATAAATTCCATGGCACTCAAATCAGCCGTGGAGCTAGGCATAGCAGATGCAATCCACAACCATGGAAAACCAATGACTCTTACTGAATTAGCTTCATCTTTGAAACTCCACCCTTCTAAAGTTAGTGTCCTGTATCGTCTCTTGCGCTTGCTAACACACAATGGTTTCTTTGCAAAAACAACACTGATGAATGGCAAAGAAGGAGAAGAAGAAACAACATATTCTCTCACTCCTCCTTCAATGCTTCTCATCAGTGGCAAATCAACATGTTTGTCACCATTTGTTACCGGATCGCTTCATCCATGTCGTCTCAACATGTGGTACTCCTCTAAGAAATGGCTCACTGAGGACAAGGAACTCAGTCTCTTTGAGAGCACAAGAGGCGAATCATTTTGGGACTATCTTAACAAAGATTCTGAATCTGATGAACTGAGTATGTTTCAAGAGGCCATGGCAGCTGATTCTCAAATCTTTAACATTGCACTAAAAGAGTATAGCCATGTGTTTGAAGGTTTGGAGTCTCTGGTTGATGTTGGAGGTGGCACAGGAGGTGTCACAAAACTTATCCATGAAACTTTTCCTCACCTAAAATGCATAGTATTTGACCAACCACAGGTTGTAAAAAACTTGTCTGGAAACGAAAATTTGAAATTTGTTGGTGGAGATATGTTCGAATCTATCCCTCCTGCAGATGCTGTTTTACTCAAGTGGATTCTGCATGATTGGAATGATGAATTGTCCTTGAAGATATTGGAGAACTGCAAAAAAGCTATTTCAGGAAAAGGGACAAAAGGAAAGGTGATAATCATTGACATATCAATTGATGAAATAAGTGACAATCATAAAACAAATGAGTTGAAATTGGACTTTGACTTCATGATGATGACTTTGTTAAATGGAAAAGAAAGAGAGAAGAAAGAATGGGAGAAACTCATATTTGACGCAGGCTTCAGCAGCTATAAGATTACACCCATATGCGGCTTCAAGTCTATCATCGAAGTTTATCCTTAA
Protein sequence

>MsG0480019533.01.T01

MNFSTNSTSSEENQLYHAQIHLYKHIYSFINSMALKSAVELGIADAIHNHGKPMTLTELASSLKLHPSKVSVLYRLLRLLTHNGFFAKTTLMNGKEGEEETTYSLTPPSMLLISGKSTCLSPFVTGSLHPCRLNMWYSSKKWLTEDKELSLFESTRGESFWDYLNKDSESDELSMFQEAMAADSQIFNIALKEYSHVFEGLESLVDVGGGTGGVTKLIHETFPHLKCIVFDQPQVVKNLSGNENLKFVGGDMFESIPPADAVLLKWILHDWNDELSLKILENCKKAISGKGTKGKVIIIDISIDEISDNHKTNELKLDFDFMMMTLLNGKEREKKEWEKLIFDAGFSSYKITPICGFKSIIEVYP*