Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480019748.01.T01 | ACJ83264.1 | 96.875 | 96 | 2 | 1 | 1 | 95 | 1 | 96 | 1.62E-58 | 187 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480019748.01.T01 | P35684 | 84.375 | 96 | 14 | 1 | 1 | 95 | 1 | 96 | 5.77E-52 | 170 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480019748.01.T01 | A0A7J7BUU3 | 95.833 | 96 | 3 | 1 | 1 | 95 | 1 | 96 | 9.99e-57 | 189 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000155.01 | MsG0480019748.01 | 0.835187 | 1.941401e-56 | 2.356279e-53 |
| MsG0180005746.01 | MsG0480019748.01 | 0.810671 | 1.026514e-50 | 6.226551e-48 |
| MsG0480019748.01 | MsG0680031628.01 | 0.804487 | 2.110094e-49 | 1.089304e-46 |
| MsG0480019748.01 | MsG0680034853.01 | 0.804327 | 2.278780e-49 | 1.171570e-46 |
| MsG0480019748.01 | MsG0780039189.01 | 0.810362 | 1.196801e-50 | 7.199730e-48 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480019748.01.T01 | MTR_1g098540 | 96.875 | 96 | 2 | 1 | 1 | 95 | 1 | 96 | 7.06e-60 | 187 |
| MsG0480019748.01.T01 | MTR_4g094398 | 93.750 | 96 | 5 | 1 | 1 | 95 | 1 | 96 | 3.46e-50 | 162 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480019748.01.T01 | AT1G61580 | 92.708 | 96 | 6 | 1 | 1 | 95 | 1 | 96 | 3.15e-50 | 163 |
| MsG0480019748.01.T01 | AT1G43170 | 89.286 | 84 | 9 | 0 | 1 | 84 | 1 | 84 | 9.38e-49 | 159 |
| MsG0480019748.01.T01 | AT1G43170 | 89.286 | 84 | 9 | 0 | 1 | 84 | 1 | 84 | 9.38e-49 | 159 |
| MsG0480019748.01.T01 | AT1G43170 | 89.286 | 84 | 9 | 0 | 1 | 84 | 1 | 84 | 9.38e-49 | 159 |
| MsG0480019748.01.T01 | AT1G43170 | 89.286 | 84 | 9 | 0 | 1 | 84 | 1 | 84 | 9.38e-49 | 159 |
| MsG0480019748.01.T01 | AT1G43170 | 89.286 | 84 | 9 | 0 | 1 | 84 | 1 | 84 | 9.38e-49 | 159 |
| MsG0480019748.01.T01 | AT1G43170 | 89.286 | 84 | 9 | 0 | 1 | 84 | 1 | 84 | 9.38e-49 | 159 |
| MsG0480019748.01.T01 | AT1G43170 | 89.286 | 84 | 9 | 0 | 1 | 84 | 1 | 84 | 9.38e-49 | 159 |
| MsG0480019748.01.T01 | AT1G43170 | 89.286 | 84 | 9 | 0 | 1 | 84 | 1 | 84 | 9.38e-49 | 159 |
Find 40 sgRNAs with CRISPR-Local
Find 36 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGCTTGGTTGGGTCATCCTT+AGG | 0.263169 | 4:+26310569 | None:intergenic |
| ACCCAAGCTAACTGCCTTCT+TGG | 0.286054 | 4:-26310546 | MsG0480019748.01.T01:CDS |
| GCACCCAAGACACGGTTCTT+TGG | 0.287363 | 4:-26310645 | MsG0480019748.01.T01:CDS |
| CACCCAAGACACGGTTCTTT+GGG | 0.292688 | 4:-26310644 | MsG0480019748.01.T01:CDS |
| TGACGAGCAGCACGCTTTCT+TGG | 0.308401 | 4:+26310614 | None:intergenic |
| GTTAGCTTGGGTGGCTTGGT+TGG | 0.310280 | 4:+26310557 | None:intergenic |
| TTCTTGTGAAGCTTGGATCC+TGG | 0.364837 | 4:+26310479 | None:intergenic |
| TTAGCTTGGGTGGCTTGGTT+GGG | 0.372233 | 4:+26310558 | None:intergenic |
| GGCAGTTAGCTTGGGTGGCT+TGG | 0.374534 | 4:+26310553 | None:intergenic |
| GCTTGGTTGGGTCATCCTTA+GGG | 0.388678 | 4:+26310570 | None:intergenic |
| GACGAGCAGCACGCTTTCTT+GGG | 0.410830 | 4:+26310615 | None:intergenic |
| AACTGCCTTCTTGGGTTACA+AGG | 0.412933 | 4:-26310537 | MsG0480019748.01.T01:CDS |
| CCCAAGCTAACTGCCTTCTT+GGG | 0.428836 | 4:-26310545 | MsG0480019748.01.T01:CDS |
| ACCCAAGAAGGCAGTTAGCT+TGG | 0.433816 | 4:+26310544 | None:intergenic |
| ATCCCAAAGAACCGTGTCTT+GGG | 0.433824 | 4:+26310642 | None:intergenic |
| ATGGTTGTTGTCGGTGTTGT+GGG | 0.440477 | 4:-26310419 | MsG0480019748.01.T01:CDS |
| AGTCTCCTTCTTGTGAAGCT+TGG | 0.450717 | 4:+26310472 | None:intergenic |
| CCCAAGAAGGCAGTTAGCTT+GGG | 0.469247 | 4:+26310545 | None:intergenic |
| TCCAGACAGTGTTCAATGTT+CGG | 0.473376 | 4:+26310373 | None:intergenic |
| TATGGTTGTTGTCGGTGTTG+TGG | 0.481177 | 4:-26310420 | MsG0480019748.01.T01:CDS |
| GACAATCATTGAACCTCCTA+TGG | 0.483779 | 4:-26310438 | MsG0480019748.01.T01:CDS |
| GCCTTCTTGGGTTACAAGGT+TGG | 0.488962 | 4:-26310533 | MsG0480019748.01.T01:CDS |
| AATCCCAAAGAACCGTGTCT+TGG | 0.489699 | 4:+26310641 | None:intergenic |
| GGAAGAAGAAGATGTCTCAC+AGG | 0.511229 | 4:-26310676 | None:intergenic |
| GTCAGAGAAGTCGAGAAGCC+AGG | 0.515408 | 4:-26310497 | MsG0480019748.01.T01:CDS |
| GAACCTCCTATGGTTGTTGT+CGG | 0.515778 | 4:-26310428 | MsG0480019748.01.T01:CDS |
| AGGATCCAAGCTTCACAAGA+AGG | 0.558613 | 4:-26310477 | MsG0480019748.01.T01:CDS |
| AAGAAGGCAGTTAGCTTGGG+TGG | 0.559172 | 4:+26310548 | None:intergenic |
| ACCAACCTTGTAACCCAAGA+AGG | 0.575488 | 4:+26310532 | None:intergenic |
| TGTTCAATGTTCGGAGACCA+CGG | 0.577689 | 4:+26310382 | None:intergenic |
| AAAAGTGAAGGCTTTCCCTA+AGG | 0.579121 | 4:-26310585 | MsG0480019748.01.T01:CDS |
| ACAACACCGACAACAACCAT+AGG | 0.591726 | 4:+26310422 | None:intergenic |
| GTTCAATGTTCGGAGACCAC+GGG | 0.593211 | 4:+26310383 | None:intergenic |
| AAGTTCGAGCACCCAAGACA+CGG | 0.616675 | 4:-26310653 | MsG0480019748.01.T01:CDS |
| ACACCGACAACAACCATAGG+AGG | 0.620644 | 4:+26310425 | None:intergenic |
| AGGAGGTTCAATGATTGTCA+CGG | 0.640790 | 4:+26310442 | None:intergenic |
| TCGTCACAGAGGAAAAGTGA+AGG | 0.670298 | 4:-26310597 | MsG0480019748.01.T01:CDS |
| GGGCTATGTGAAAACTCCCG+TGG | 0.677832 | 4:-26310399 | MsG0480019748.01.T01:CDS |
| AAGCGTGCTGCTCGTCACAG+AGG | 0.692287 | 4:-26310608 | MsG0480019748.01.T01:CDS |
| TCACAAGAAGGAGACTTGTG+AGG | 0.693775 | 4:-26310465 | MsG0480019748.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAAAGTGAAGGCTTTCCCTA+AGG | - | Chr4:26310465-26310484 | MsG0480019748.01.T01:CDS | 40.0% | |
| AGGAGGTTCAATGATTGTCA+CGG | + | Chr4:26310611-26310630 | None:intergenic | 40.0% | |
| GACAATCATTGAACCTCCTA+TGG | - | Chr4:26310612-26310631 | MsG0480019748.01.T01:CDS | 40.0% | |
| AACTGCCTTCTTGGGTTACA+AGG | - | Chr4:26310513-26310532 | MsG0480019748.01.T01:CDS | 45.0% | |
| AATCCCAAAGAACCGTGTCT+TGG | + | Chr4:26310412-26310431 | None:intergenic | 45.0% | |
| ACAACACCGACAACAACCAT+AGG | + | Chr4:26310631-26310650 | None:intergenic | 45.0% | |
| ACCAACCTTGTAACCCAAGA+AGG | + | Chr4:26310521-26310540 | None:intergenic | 45.0% | |
| AGGATCCAAGCTTCACAAGA+AGG | - | Chr4:26310573-26310592 | MsG0480019748.01.T01:CDS | 45.0% | |
| AGTCTCCTTCTTGTGAAGCT+TGG | + | Chr4:26310581-26310600 | None:intergenic | 45.0% | |
| ATCCCAAAGAACCGTGTCTT+GGG | + | Chr4:26310411-26310430 | None:intergenic | 45.0% | |
| GAACCTCCTATGGTTGTTGT+CGG | - | Chr4:26310622-26310641 | MsG0480019748.01.T01:CDS | 45.0% | |
| TCACAAGAAGGAGACTTGTG+AGG | - | Chr4:26310585-26310604 | MsG0480019748.01.T01:CDS | 45.0% | |
| TCGTCACAGAGGAAAAGTGA+AGG | - | Chr4:26310453-26310472 | MsG0480019748.01.T01:CDS | 45.0% | |
| TTCTTGTGAAGCTTGGATCC+TGG | + | Chr4:26310574-26310593 | None:intergenic | 45.0% | |
| !! | ATGGTTGTTGTCGGTGTTGT+GGG | - | Chr4:26310631-26310650 | MsG0480019748.01.T01:CDS | 45.0% |
| !! | TATGGTTGTTGTCGGTGTTG+TGG | - | Chr4:26310630-26310649 | MsG0480019748.01.T01:CDS | 45.0% |
| AAGTTCGAGCACCCAAGACA+CGG | - | Chr4:26310397-26310416 | MsG0480019748.01.T01:CDS | 50.0% | |
| ACACCGACAACAACCATAGG+AGG | + | Chr4:26310628-26310647 | None:intergenic | 50.0% | |
| ACCCAAGAAGGCAGTTAGCT+TGG | + | Chr4:26310509-26310528 | None:intergenic | 50.0% | |
| ACCCAAGCTAACTGCCTTCT+TGG | - | Chr4:26310504-26310523 | MsG0480019748.01.T01:CDS | 50.0% | |
| CACCCAAGACACGGTTCTTT+GGG | - | Chr4:26310406-26310425 | MsG0480019748.01.T01:CDS | 50.0% | |
| CCCAAGCTAACTGCCTTCTT+GGG | - | Chr4:26310505-26310524 | MsG0480019748.01.T01:CDS | 50.0% | |
| GCCTTCTTGGGTTACAAGGT+TGG | - | Chr4:26310517-26310536 | MsG0480019748.01.T01:CDS | 50.0% | |
| GCTTGGTTGGGTCATCCTTA+GGG | + | Chr4:26310483-26310502 | None:intergenic | 50.0% | |
| ! | AAGAAGGCAGTTAGCTTGGG+TGG | + | Chr4:26310505-26310524 | None:intergenic | 50.0% |
| ! | CCCAAGAAGGCAGTTAGCTT+GGG | + | Chr4:26310508-26310527 | None:intergenic | 50.0% |
| !! | TTAGCTTGGGTGGCTTGGTT+GGG | + | Chr4:26310495-26310514 | None:intergenic | 50.0% |
| GACGAGCAGCACGCTTTCTT+GGG | + | Chr4:26310438-26310457 | None:intergenic | 55.0% | |
| GCACCCAAGACACGGTTCTT+TGG | - | Chr4:26310405-26310424 | MsG0480019748.01.T01:CDS | 55.0% | |
| GGGCTATGTGAAAACTCCCG+TGG | - | Chr4:26310651-26310670 | MsG0480019748.01.T01:CDS | 55.0% | |
| GTCAGAGAAGTCGAGAAGCC+AGG | - | Chr4:26310553-26310572 | MsG0480019748.01.T01:CDS | 55.0% | |
| TGACGAGCAGCACGCTTTCT+TGG | + | Chr4:26310439-26310458 | None:intergenic | 55.0% | |
| ! | GGCTTGGTTGGGTCATCCTT+AGG | + | Chr4:26310484-26310503 | None:intergenic | 55.0% |
| !! | GTTAGCTTGGGTGGCTTGGT+TGG | + | Chr4:26310496-26310515 | None:intergenic | 55.0% |
| ! | AAGCGTGCTGCTCGTCACAG+AGG | - | Chr4:26310442-26310461 | MsG0480019748.01.T01:CDS | 60.0% |
| ! | GGCAGTTAGCTTGGGTGGCT+TGG | + | Chr4:26310500-26310519 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 26310385 | 26310687 | 26310385 | ID=MsG0480019748.01;Name=MsG0480019748.01 |
| Chr4 | mRNA | 26310385 | 26310687 | 26310385 | ID=MsG0480019748.01.T01;Parent=MsG0480019748.01;Name=MsG0480019748.01.T01;_AED=0.39;_eAED=0.39;_QI=0|-1|0|1|-1|1|1|0|100 |
| Chr4 | exon | 26310385 | 26310687 | 26310385 | ID=MsG0480019748.01.T01:exon:9086;Parent=MsG0480019748.01.T01 |
| Chr4 | CDS | 26310385 | 26310687 | 26310385 | ID=MsG0480019748.01.T01:cds;Parent=MsG0480019748.01.T01 |
| Gene Sequence |
| Protein sequence |
