AlfalfaGEDB | MsG0480019874.01

M. sativa cultivar ZhongmuNo.1 / MsG0480019874.01

KEGG: K03025
GO: GO:0005666 GO:0006383
PFAM: PF05158

Nr:

Query id	Subject id	identity %	alignment length	mis matches	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480019874.01.T01	XP_024626527.1	96.104	231	9	1	231	1	231	8.89E-164	461
MsG0480019874.01.T03	XP_024626527.1	97.11	173	5	1	173	59	231	2.35E-121	352
MsG0480019874.01.T02	XP_024626527.1	96.277	188	7	1	188	44	231	1.25E-132	381

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480019874.01.T01	Q921X6	25.926	243	151	5	12	231	80	316	1.86E-23	99
MsG0480019874.01.T02	Q921X6	25.118	211	129	5	1	188	112	316	2.86E-17	80.9
MsG0480019874.01.T03	Q86JM3	26.203	187	108	4	1	172	114	285	7.14E-15	73.6

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480019874.01.T01	A0A396GUA7	96.104	231	9	1	231	1	231	4.25e-164	461
MsG0480019874.01.T02	A0A396GUA7	96.277	188	7	1	188	44	231	5.97e-133	381
MsG0480019874.01.T03	A0A396GUA7	97.110	173	5	1	173	59	231	1.12e-121	352

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0480019873.01	MsG0480019874.01	0.856157	3.890298e-62	9.198267e-59
MsG0480019874.01	MsG0680031154.01	0.802364	5.809444e-49	2.840559e-46

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480019874.01.T01	MTR_0003s0690	96.532	173	6	0	59	231	1	173	2.19e-124	350
MsG0480019874.01.T01	MTR_7g109780	74.093	193	45	2	44	231	1	193	1.06e-102	296
MsG0480019874.01.T01	MTR_7g109760	43.976	166	36	5	1	164	1	111	1.49e-32	114
MsG0480019874.01.T03	MTR_0003s0690	96.532	173	6	0	1	173	1	173	1.47e-123	345
MsG0480019874.01.T03	MTR_7g109780	74.719	178	40	2	1	173	16	193	5.50e-94	271
MsG0480019874.01.T03	MTR_7g109760	49.533	107	26	3	1	106	32	111	2.65e-26	97.1
MsG0480019874.01.T02	MTR_0003s0690	96.532	173	6	0	16	188	1	173	7.09e-124	347
MsG0480019874.01.T02	MTR_7g109780	74.093	193	45	2	1	188	1	193	1.62e-102	293
MsG0480019874.01.T02	MTR_7g109760	49.533	107	26	3	16	121	32	111	4.39e-26	96.7

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480019874.01.T01	AT5G23710	37.209	215	128	3	21	231	19	230	7.65e-47	155
MsG0480019874.01.T03	AT5G23710	39.548	177	101	2	1	173	56	230	3.76e-40	135
MsG0480019874.01.T02	AT5G23710	38.021	192	113	2	1	188	41	230	1.93e-43	145

Find 37 sgRNAs with CRISPR-Local

Find 54 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CAGATGACAAGTACTGGATA+TGG	0.343879	4:+28841222	MsG0480019874.01.T01:CDS
ATGAAACGTTTGCAAGAATC+TGG	0.362818	4:+28840730	MsG0480019874.01.T01:CDS
GATGTTTGGAGTGGGTTAAA+AGG	0.362955	4:+28841118	MsG0480019874.01.T01:CDS
GTGCTTGGAATGCATTTCAT+TGG	0.385524	4:+28841074	MsG0480019874.01.T01:CDS
GAGCCTTCAAAGGATATAAC+TGG	0.386052	4:+28840991	MsG0480019874.01.T01:CDS
CCACCAGTTATATCCTTTGA+AGG	0.401211	4:-28840994	None:intergenic
TTCCACATGTGATGGATGTT+TGG	0.402495	4:+28841104	MsG0480019874.01.T01:CDS
TAGAGTATACGCTCTTCATT+TGG	0.409796	4:-28840790	None:intergenic
GGCACTTATGAACAAGAGTA+TGG	0.416971	4:+28840909	MsG0480019874.01.T01:CDS
CATGTGTCTATTATCCGAAA+TGG	0.443441	4:+28841391	MsG0480019874.01.T01:CDS
CGCTCTAAAGAAAACGTGCT+TGG	0.454247	4:+28841059	MsG0480019874.01.T01:CDS
GGATAATAGACACATGTTGT+TGG	0.476483	4:-28841384	None:intergenic
TGGAGTGGGTTAAAAGGAGT+GGG	0.480413	4:+28841124	MsG0480019874.01.T01:CDS
AGATGACAAGTACTGGATAT+GGG	0.485810	4:+28841223	MsG0480019874.01.T01:CDS
CAAAGGTATAAAGATCTACA+TGG	0.502338	4:+28840957	MsG0480019874.01.T01:CDS
ATCATGCAGATGACAAGTAC+TGG	0.502476	4:+28841216	MsG0480019874.01.T01:CDS
CAGCGAAGGGAGTCTTGATA+CGG	0.510725	4:+28841026	MsG0480019874.01.T01:CDS
GTCTTCACAACTCAAGTCAC+TGG	0.514655	4:+28841147	MsG0480019874.01.T01:CDS
TGTCTATTATCCGAAATGGT+TGG	0.528005	4:+28841395	MsG0480019874.01.T01:CDS
ATACTTAGCAAGGAAAACTC+AGG	0.536886	4:+28840820	MsG0480019874.01.T01:CDS
AACAGAATTTGAGCCTTCAA+AGG	0.546675	4:+28840981	MsG0480019874.01.T01:CDS
TATGAACAAGAGTATGGTAA+AGG	0.546896	4:+28840915	MsG0480019874.01.T01:CDS
TATTAAGCACAGCAATAGGA+AGG	0.562016	4:-28840876	None:intergenic
TTGGAGTGGGTTAAAAGGAG+TGG	0.562912	4:+28841123	MsG0480019874.01.T01:CDS
GAGAGAGTTTCCACATGTGA+TGG	0.576485	4:+28841096	MsG0480019874.01.T01:CDS
TTGTTACATATGCAAAAGCA+AGG	0.588438	4:+28841275	MsG0480019874.01.T01:CDS
CTATGACCTTATACTTAGCA+AGG	0.601401	4:+28840810	MsG0480019874.01.T01:CDS
CCTTCAAAGGATATAACTGG+TGG	0.613871	4:+28840994	MsG0480019874.01.T01:CDS
CTCCAAACATCCATCACATG+TGG	0.618959	4:-28841106	None:intergenic
TACTTAGCAAGGAAAACTCA+GGG	0.624253	4:+28840821	MsG0480019874.01.T01:CDS
GATGACAAGTACTGGATATG+GGG	0.631855	4:+28841224	MsG0480019874.01.T01:CDS
GGAGTGGGTTAAAAGGAGTG+GGG	0.632175	4:+28841125	MsG0480019874.01.T01:CDS
CATGTGATGGATGTTTGGAG+TGG	0.639686	4:+28841109	MsG0480019874.01.T01:CDS
ATGTGATGGATGTTTGGAGT+GGG	0.644389	4:+28841110	MsG0480019874.01.T01:CDS
TGTTACATATGCAAAAGCAA+GGG	0.651007	4:+28841276	MsG0480019874.01.T01:CDS
AAAGGTATAAAGATCTACAT+GGG	0.652631	4:+28840958	MsG0480019874.01.T01:CDS
CTTCAAAGGATATAACTGGT+GGG	0.673279	4:+28840995	MsG0480019874.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TGTGCTTAATAAAAATATGA+AGG	+	Chr4:28840888-28840907	MsG0480019874.01.T01:CDS	20.0%
!!!	CTTTATACCTTTGTTTTTAA+TGG	-	Chr4:28840950-28840969	None:intergenic	20.0%
!!	AAAGGTATAAAGATCTACAT+GGG	+	Chr4:28840958-28840977	MsG0480019874.01.T01:CDS	25.0%
!!	TTTTTATTAAGCACAGCAAT+AGG	-	Chr4:28840883-28840902	None:intergenic	25.0%
!!!	ATACCTTTGTTTTTAATGGA+AGG	-	Chr4:28840946-28840965	None:intergenic	25.0%
	CTTCCTTCCATTAAAAACAA+AGG	+	Chr4:28840940-28840959	MsG0480019874.01.T01:CDS	30.0%
	TGACAAGAAAAACATGGAAA+GGG	-	Chr4:28841327-28841346	None:intergenic	30.0%
	TGTTACATATGCAAAAGCAA+GGG	+	Chr4:28841276-28841295	MsG0480019874.01.T01:CDS	30.0%
	TTGACAAGAAAAACATGGAA+AGG	-	Chr4:28841328-28841347	None:intergenic	30.0%
	TTGTTACATATGCAAAAGCA+AGG	+	Chr4:28841275-28841294	MsG0480019874.01.T01:CDS	30.0%
!	AGAAGAGATTTTGCAAACTT+TGG	+	Chr4:28841179-28841198	MsG0480019874.01.T01:CDS	30.0%
!	AGTTTTCCTTGCTAAGTATA+AGG	-	Chr4:28840819-28840838	None:intergenic	30.0%
!	CAAAGGTATAAAGATCTACA+TGG	+	Chr4:28840957-28840976	MsG0480019874.01.T01:CDS	30.0%
!	TATGAACAAGAGTATGGTAA+AGG	+	Chr4:28840915-28840934	MsG0480019874.01.T01:CDS	30.0%
!	TTCCATGTTTTTCTTGTCAA+CGG	+	Chr4:28841328-28841347	MsG0480019874.01.T01:CDS	30.0%
	AACAGAATTTGAGCCTTCAA+AGG	+	Chr4:28840981-28841000	MsG0480019874.01.T01:CDS	35.0%
	ATACTTAGCAAGGAAAACTC+AGG	+	Chr4:28840820-28840839	MsG0480019874.01.T01:CDS	35.0%
	ATCCGTTGACAAGAAAAACA+TGG	-	Chr4:28841333-28841352	None:intergenic	35.0%
	ATGAAACGTTTGCAAGAATC+TGG	+	Chr4:28840730-28840749	MsG0480019874.01.T01:CDS	35.0%
	CATGTGTCTATTATCCGAAA+TGG	+	Chr4:28841391-28841410	MsG0480019874.01.T01:CDS	35.0%
	CTATGACCTTATACTTAGCA+AGG	+	Chr4:28840810-28840829	MsG0480019874.01.T01:CDS	35.0%
	CTTCAAAGGATATAACTGGT+GGG	+	Chr4:28840995-28841014	MsG0480019874.01.T01:CDS	35.0%
	GGATAATAGACACATGTTGT+TGG	-	Chr4:28841387-28841406	None:intergenic	35.0%
	TACTTAGCAAGGAAAACTCA+GGG	+	Chr4:28840821-28840840	MsG0480019874.01.T01:CDS	35.0%
	TAGAGTATACGCTCTTCATT+TGG	-	Chr4:28840793-28840812	None:intergenic	35.0%
	TATTAAGCACAGCAATAGGA+AGG	-	Chr4:28840879-28840898	None:intergenic	35.0%
	TGTCTATTATCCGAAATGGT+TGG	+	Chr4:28841395-28841414	MsG0480019874.01.T01:CDS	35.0%
!	ATGAGCTTTTGTTCAACAGA+TGG	+	Chr4:28841351-28841370	MsG0480019874.01.T01:CDS	35.0%
!	ATGTAACAAGTTTTGCCAAC+AGG	-	Chr4:28841264-28841283	None:intergenic	35.0%
!	GATTTTGCATCTATTCCTGT+TGG	+	Chr4:28841246-28841265	MsG0480019874.01.T01:CDS	35.0%
!!	AGATGACAAGTACTGGATAT+GGG	+	Chr4:28841223-28841242	MsG0480019874.01.T01:CDS	35.0%
!!	TTTTTGTCGTTTCTGTTGAG+AGG	-	Chr4:28840758-28840777	None:intergenic	35.0%
	ATCATGCAGATGACAAGTAC+TGG	+	Chr4:28841216-28841235	MsG0480019874.01.T01:CDS	40.0%
	CCACCAGTTATATCCTTTGA+AGG	-	Chr4:28840997-28841016	None:intergenic	40.0%
	CCTTCAAAGGATATAACTGG+TGG	+	Chr4:28840994-28841013	MsG0480019874.01.T01:CDS	40.0%
	GAGCCTTCAAAGGATATAAC+TGG	+	Chr4:28840991-28841010	MsG0480019874.01.T01:CDS	40.0%
	GGCACTTATGAACAAGAGTA+TGG	+	Chr4:28840909-28840928	MsG0480019874.01.T01:CDS	40.0%
	GTGCTTGGAATGCATTTCAT+TGG	+	Chr4:28841074-28841093	MsG0480019874.01.T01:CDS	40.0%
!!	ATGTGATGGATGTTTGGAGT+GGG	+	Chr4:28841110-28841129	MsG0480019874.01.T01:CDS	40.0%
!!	CAGATGACAAGTACTGGATA+TGG	+	Chr4:28841222-28841241	MsG0480019874.01.T01:CDS	40.0%
!!	GATGACAAGTACTGGATATG+GGG	+	Chr4:28841224-28841243	MsG0480019874.01.T01:CDS	40.0%
!!	GATGTTTGGAGTGGGTTAAA+AGG	+	Chr4:28841118-28841137	MsG0480019874.01.T01:CDS	40.0%
!!	TTCCACATGTGATGGATGTT+TGG	+	Chr4:28841104-28841123	MsG0480019874.01.T01:CDS	40.0%
	CTCCAAACATCCATCACATG+TGG	-	Chr4:28841109-28841128	None:intergenic	45.0%
	GAGAGAGTTTCCACATGTGA+TGG	+	Chr4:28841096-28841115	MsG0480019874.01.T01:CDS	45.0%
	GTCTTCACAACTCAAGTCAC+TGG	+	Chr4:28841147-28841166	MsG0480019874.01.T01:CDS	45.0%
!	GTGGGCATTTTTACAGCGAA+GGG	+	Chr4:28841013-28841032	MsG0480019874.01.T01:CDS	45.0%
!!	CATGTGATGGATGTTTGGAG+TGG	+	Chr4:28841109-28841128	MsG0480019874.01.T01:CDS	45.0%
!!	CGCTCTAAAGAAAACGTGCT+TGG	+	Chr4:28841059-28841078	MsG0480019874.01.T01:CDS	45.0%
!!	TGGAGTGGGTTAAAAGGAGT+GGG	+	Chr4:28841124-28841143	MsG0480019874.01.T01:CDS	45.0%
!!	TTGGAGTGGGTTAAAAGGAG+TGG	+	Chr4:28841123-28841142	MsG0480019874.01.T01:CDS	45.0%
	CAGCGAAGGGAGTCTTGATA+CGG	+	Chr4:28841026-28841045	MsG0480019874.01.T01:CDS	50.0%
!	GGTGGGCATTTTTACAGCGA+AGG	+	Chr4:28841012-28841031	MsG0480019874.01.T01:CDS	50.0%
!!	GGAGTGGGTTAAAAGGAGTG+GGG	+	Chr4:28841125-28841144	MsG0480019874.01.T01:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
Chr4	gene	28840730	28841425	28840730	ID=MsG0480019874.01;Name=MsG0480019874.01
Chr4	mRNA	28840730	28841425	28840730	ID=MsG0480019874.01.T01;Parent=MsG0480019874.01;Name=MsG0480019874.01.T01;_AED=0.00;_eAED=0.00;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|231
Chr4	exon	28840730	28841425	28840730	ID=MsG0480019874.01.T01:exon:12026;Parent=MsG0480019874.01.T01
Chr4	CDS	28840730	28841425	28840730	ID=MsG0480019874.01.T01:cds;Parent=MsG0480019874.01.T01
Chr4	mRNA	28840859	28841425	28840859	ID=MsG0480019874.01.T02;Parent=MsG0480019874.01;Name=MsG0480019874.01.T02;_AED=0.10;_eAED=0.10;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|188
Chr4	exon	28840859	28841425	28840859	ID=MsG0480019874.01.T02:exon:12027;Parent=MsG0480019874.01.T02
Chr4	CDS	28840859	28841425	28840859	ID=MsG0480019874.01.T02:cds;Parent=MsG0480019874.01.T02
Chr4	mRNA	28840904	28841425	28840904	ID=MsG0480019874.01.T03;Parent=MsG0480019874.01;Name=MsG0480019874.01.T03;_AED=0.13;_eAED=0.13;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|173
Chr4	exon	28840904	28841425	28840904	ID=MsG0480019874.01.T03:exon:12028;Parent=MsG0480019874.01.T03
Chr4	CDS	28840904	28841425	28840904	ID=MsG0480019874.01.T03:cds;Parent=MsG0480019874.01.T03

Gene Sequence

>MsG0480019874.01.T01

ATGAAACGTTTGCAAGAATCTGGTTCCTCTCAACAGAAACGACAAAAACTGTCGTCGCTGCCAAATGAAGAGCGTATACTCTATGACCTTATACTTAGCAAGGAAAACTCAGGGATCTCAAAAACAGACATGAGAAGAGAAACAAACCTTCCTATTGCTGTGCTTAATAAAAATATGAAGGCACTTATGAACAAGAGTATGGTAAAGGAACTTCCTTCCATTAAAAACAAAGGTATAAAGATCTACATGGGAACAGAATTTGAGCCTTCAAAGGATATAACTGGTGGGCATTTTTACAGCGAAGGGAGTCTTGATACGGTTTTTATAGACGCTCTAAAGAAAACGTGCTTGGAATGCATTTCATTGGAGAGAGTTTCCACATGTGATGGATGTTTGGAGTGGGTTAAAAGGAGTGGGGTCTTCACAACTCAAGTCACTGGAGAACAAATAGAAGAGATTTTGCAAACTTTGGTTTTAGATGATGAGATCATGCAGATGACAAGTACTGGATATGGGGATTTTGCATCTATTCCTGTTGGCAAAACTTGTTACATATGCAAAAGCAAGGGTGAAAAGAAATCTGCTGACTCGACTCCCTTTCCATGTTTTTCTTGTCAACGGATGAGCTTTTGTTCAACAGATGGCGTTGTCTCTCCAACAACATGTGTCTATTATCCGAAATGGTTGGATTTTTGA

>MsG0480019874.01.T03

ATGAAGGCACTTATGAACAAGAGTATGGTAAAGGAACTTCCTTCCATTAAAAACAAAGGTATAAAGATCTACATGGGAACAGAATTTGAGCCTTCAAAGGATATAACTGGTGGGCATTTTTACAGCGAAGGGAGTCTTGATACGGTTTTTATAGACGCTCTAAAGAAAACGTGCTTGGAATGCATTTCATTGGAGAGAGTTTCCACATGTGATGGATGTTTGGAGTGGGTTAAAAGGAGTGGGGTCTTCACAACTCAAGTCACTGGAGAACAAATAGAAGAGATTTTGCAAACTTTGGTTTTAGATGATGAGATCATGCAGATGACAAGTACTGGATATGGGGATTTTGCATCTATTCCTGTTGGCAAAACTTGTTACATATGCAAAAGCAAGGGTGAAAAGAAATCTGCTGACTCGACTCCCTTTCCATGTTTTTCTTGTCAACGGATGAGCTTTTGTTCAACAGATGGCGTTGTCTCTCCAACAACATGTGTCTATTATCCGAAATGGTTGGATTTTTGA

>MsG0480019874.01.T02

ATGAGAAGAGAAACAAACCTTCCTATTGCTGTGCTTAATAAAAATATGAAGGCACTTATGAACAAGAGTATGGTAAAGGAACTTCCTTCCATTAAAAACAAAGGTATAAAGATCTACATGGGAACAGAATTTGAGCCTTCAAAGGATATAACTGGTGGGCATTTTTACAGCGAAGGGAGTCTTGATACGGTTTTTATAGACGCTCTAAAGAAAACGTGCTTGGAATGCATTTCATTGGAGAGAGTTTCCACATGTGATGGATGTTTGGAGTGGGTTAAAAGGAGTGGGGTCTTCACAACTCAAGTCACTGGAGAACAAATAGAAGAGATTTTGCAAACTTTGGTTTTAGATGATGAGATCATGCAGATGACAAGTACTGGATATGGGGATTTTGCATCTATTCCTGTTGGCAAAACTTGTTACATATGCAAAAGCAAGGGTGAAAAGAAATCTGCTGACTCGACTCCCTTTCCATGTTTTTCTTGTCAACGGATGAGCTTTTGTTCAACAGATGGCGTTGTCTCTCCAACAACATGTGTCTATTATCCGAAATGGTTGGATTTTTGA

Protein sequence

>MsG0480019874.01.T01

MKRLQESGSSQQKRQKLSSLPNEERILYDLILSKENSGISKTDMRRETNLPIAVLNKNMKALMNKSMVKELPSIKNKGIKIYMGTEFEPSKDITGGHFYSEGSLDTVFIDALKKTCLECISLERVSTCDGCLEWVKRSGVFTTQVTGEQIEEILQTLVLDDEIMQMTSTGYGDFASIPVGKTCYICKSKGEKKSADSTPFPCFSCQRMSFCSTDGVVSPTTCVYYPKWLDF*

>MsG0480019874.01.T03

MKALMNKSMVKELPSIKNKGIKIYMGTEFEPSKDITGGHFYSEGSLDTVFIDALKKTCLECISLERVSTCDGCLEWVKRSGVFTTQVTGEQIEEILQTLVLDDEIMQMTSTGYGDFASIPVGKTCYICKSKGEKKSADSTPFPCFSCQRMSFCSTDGVVSPTTCVYYPKWLDF*

>MsG0480019874.01.T02

MRRETNLPIAVLNKNMKALMNKSMVKELPSIKNKGIKIYMGTEFEPSKDITGGHFYSEGSLDTVFIDALKKTCLECISLERVSTCDGCLEWVKRSGVFTTQVTGEQIEEILQTLVLDDEIMQMTSTGYGDFASIPVGKTCYICKSKGEKKSADSTPFPCFSCQRMSFCSTDGVVSPTTCVYYPKWLDF*