Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480019873.01.T01 | XP_024626527.1 | 96.491 | 114 | 4 | 0 | 9 | 122 | 118 | 231 | 5.37E-75 | 232 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480019873.01.T01 | A0A396GUA7 | 96.491 | 114 | 4 | 0 | 9 | 122 | 118 | 231 | 2.57e-75 | 232 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048856.01 | MsG0480019873.01 | 0.803834 | 2.885140e-49 | 1.464712e-46 |
| MsG0180000470.01 | MsG0480019873.01 | 0.834002 | 3.854237e-56 | 4.514727e-53 |
| MsG0180001892.01 | MsG0480019873.01 | 0.809667 | 1.689500e-50 | 9.980575e-48 |
| MsG0180002089.01 | MsG0480019873.01 | 0.803369 | 3.602730e-49 | 1.807176e-46 |
| MsG0180002259.01 | MsG0480019873.01 | 0.828347 | 9.457380e-55 | 9.374949e-52 |
| MsG0180003669.01 | MsG0480019873.01 | 0.807452 | 5.019069e-50 | 2.796678e-47 |
| MsG0180004275.01 | MsG0480019873.01 | 0.822827 | 1.923715e-53 | 1.628133e-50 |
| MsG0280006324.01 | MsG0480019873.01 | 0.803488 | 3.403670e-49 | 1.712641e-46 |
| MsG0280010796.01 | MsG0480019873.01 | 0.809552 | 1.788091e-50 | 1.053059e-47 |
| MsG0380011609.01 | MsG0480019873.01 | 0.808419 | 3.126738e-50 | 1.786916e-47 |
| MsG0380012009.01 | MsG0480019873.01 | 0.811821 | 5.779607e-51 | 3.613972e-48 |
| MsG0380013475.01 | MsG0480019873.01 | 0.802838 | 4.639585e-49 | 2.296115e-46 |
| MsG0380014495.01 | MsG0480019873.01 | 0.814266 | 1.681777e-51 | 1.123211e-48 |
| MsG0480019873.01 | MsG0480019874.01 | 0.856157 | 3.890298e-62 | 9.198267e-59 |
| MsG0480019873.01 | MsG0480022068.01 | 0.800424 | 1.450905e-48 | 6.753593e-46 |
| MsG0480019873.01 | MsG0580024038.01 | 0.807168 | 5.766468e-50 | 3.189338e-47 |
| MsG0480019873.01 | MsG0580025135.01 | 0.813846 | 2.081450e-51 | 1.374371e-48 |
| MsG0480019873.01 | MsG0580026002.01 | 0.807606 | 4.656431e-50 | 2.604799e-47 |
| MsG0480019873.01 | MsG0580027922.01 | 0.821949 | 3.076366e-53 | 2.539149e-50 |
| MsG0480019873.01 | MsG0680030882.01 | 0.801291 | 9.649448e-49 | 4.591247e-46 |
| MsG0480019873.01 | MsG0680031379.01 | 0.824757 | 6.787601e-54 | 6.068375e-51 |
| MsG0480019873.01 | MsG0680031505.01 | 0.802697 | 4.961954e-49 | 2.446718e-46 |
| MsG0480019873.01 | MsG0680035346.01 | 0.801439 | 9.001266e-49 | 4.298737e-46 |
| MsG0480019873.01 | MsG0780036080.01 | 0.802455 | 5.565977e-49 | 2.727685e-46 |
| MsG0480019873.01 | MsG0780036477.01 | 0.811587 | 6.499451e-51 | 4.038894e-48 |
| MsG0480019873.01 | MsG0780037054.01 | 0.831461 | 1.647907e-55 | 1.789525e-52 |
| MsG0480019873.01 | MsG0780039423.01 | 0.802082 | 6.641728e-49 | 3.224065e-46 |
| MsG0480019873.01 | MsG0880042966.01 | 0.801820 | 7.518193e-49 | 3.625302e-46 |
| MsG0480019873.01 | MsG0880043479.01 | 0.805603 | 1.232476e-49 | 6.548059e-47 |
| MsG0480019873.01 | MsG0880044210.01 | 0.805165 | 1.523065e-49 | 8.001468e-47 |
| MsG0480019873.01 | MsG0880044323.01 | 0.807274 | 5.475832e-50 | 3.037040e-47 |
| MsG0480019873.01 | MsG0880044415.01 | 0.801357 | 9.353214e-49 | 4.457754e-46 |
| MsG0480019873.01 | MsG0880044416.01 | 0.836031 | 1.187383e-56 | 1.478084e-53 |
| MsG0480019873.01 | MsG0880045362.01 | 0.809111 | 2.223237e-50 | 1.294021e-47 |
| MsG0480019873.01 | MsG0880047473.01 | 0.806639 | 7.459786e-50 | 4.069550e-47 |
| MsG0380015720.01 | MsG0480019873.01 | 0.805683 | 1.185858e-49 | 6.313709e-47 |
| MsG0380016910.01 | MsG0480019873.01 | 0.806926 | 6.487853e-50 | 3.565841e-47 |
| MsG0380017326.01 | MsG0480019873.01 | 0.814053 | 1.873610e-51 | 1.244102e-48 |
| MsG0480018163.01 | MsG0480019873.01 | 0.804756 | 1.853840e-49 | 9.637747e-47 |
| MsG0280007955.01 | MsG0480019873.01 | 0.829314 | 5.516118e-55 | 5.626335e-52 |
| MsG0280008615.01 | MsG0480019873.01 | 0.826793 | 2.232506e-54 | 2.114961e-51 |
| MsG0280010443.01 | MsG0480019873.01 | 0.808201 | 3.479926e-50 | 1.977453e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480019873.01.T01 | MTR_0003s0690 | 94.737 | 114 | 6 | 0 | 9 | 122 | 60 | 173 | 5.62e-77 | 225 |
| MsG0480019873.01.T01 | MTR_7g109780 | 77.119 | 118 | 23 | 1 | 9 | 122 | 76 | 193 | 3.85e-62 | 188 |
| MsG0480019873.01.T01 | MTR_7g109760 | 70.213 | 47 | 14 | 0 | 9 | 55 | 65 | 111 | 1.23e-20 | 80.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480019873.01.T01 | AT5G23710 | 43.478 | 92 | 48 | 1 | 35 | 122 | 139 | 230 | 4.85e-22 | 87.4 |
Find 19 sgRNAs with CRISPR-Local
Find 31 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAGATGACAAGTACTGGATA+TGG | 0.343879 | 4:-28838264 | MsG0480019873.01.T01:CDS |
| GATGTTTGGAGTGGGTTAAA+AGG | 0.362955 | 4:-28838368 | MsG0480019873.01.T01:CDS |
| TTCCACATGTGATGGATGTT+TGG | 0.402495 | 4:-28838382 | MsG0480019873.01.T01:CDS |
| CATGTGTCTATTATCCGAAA+TGG | 0.443441 | 4:-28838095 | MsG0480019873.01.T01:CDS |
| GGATAATAGACACATGTTGT+TGG | 0.476483 | 4:+28838102 | None:intergenic |
| TGGAGTGGGTTAAAAGGAGT+GGG | 0.480413 | 4:-28838362 | MsG0480019873.01.T01:CDS |
| AGATGACAAGTACTGGATAT+GGG | 0.485810 | 4:-28838263 | MsG0480019873.01.T01:CDS |
| ATCATGCAGATGACAAGTAC+TGG | 0.502476 | 4:-28838270 | MsG0480019873.01.T01:CDS |
| GTCTTCACAACTCAAGTCAC+TGG | 0.514655 | 4:-28838339 | MsG0480019873.01.T01:CDS |
| TGTCTATTATCCGAAATGGT+TGG | 0.528005 | 4:-28838091 | MsG0480019873.01.T01:CDS |
| TTGGAGTGGGTTAAAAGGAG+TGG | 0.562912 | 4:-28838363 | MsG0480019873.01.T01:CDS |
| GAGAGAGTTTCCACATGTGA+TGG | 0.576485 | 4:-28838390 | MsG0480019873.01.T01:CDS |
| TTGTTACATATGCAAAAGCA+AGG | 0.588438 | 4:-28838211 | MsG0480019873.01.T01:CDS |
| CTCCAAACATCCATCACATG+TGG | 0.618959 | 4:+28838380 | None:intergenic |
| GATGACAAGTACTGGATATG+GGG | 0.631855 | 4:-28838262 | MsG0480019873.01.T01:CDS |
| GGAGTGGGTTAAAAGGAGTG+GGG | 0.632175 | 4:-28838361 | MsG0480019873.01.T01:CDS |
| CATGTGATGGATGTTTGGAG+TGG | 0.639686 | 4:-28838377 | MsG0480019873.01.T01:CDS |
| ATGTGATGGATGTTTGGAGT+GGG | 0.644389 | 4:-28838376 | MsG0480019873.01.T01:CDS |
| TGTTACATATGCAAAAGCAA+GGG | 0.651007 | 4:-28838210 | MsG0480019873.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TGACAAGAAAAACATGGAAA+GGG | + | Chr4:28838353-28838372 | None:intergenic | 30.0% | |
| TGTTACATATGCAAAAGCAA+GGG | - | Chr4:28838302-28838321 | MsG0480019873.01.T01:CDS | 30.0% | |
| TTGACAAGAAAAACATGGAA+AGG | + | Chr4:28838354-28838373 | None:intergenic | 30.0% | |
| TTGTTACATATGCAAAAGCA+AGG | - | Chr4:28838301-28838320 | MsG0480019873.01.T01:CDS | 30.0% | |
| ! | AGAAGAGATTTTGCAAACTT+TGG | - | Chr4:28838205-28838224 | MsG0480019873.01.T01:CDS | 30.0% |
| ! | TTCCATGTTTTTCTTGTCAA+CGG | - | Chr4:28838354-28838373 | MsG0480019873.01.T01:CDS | 30.0% |
| ATCCGTTGACAAGAAAAACA+TGG | + | Chr4:28838359-28838378 | None:intergenic | 35.0% | |
| CATGTGTCTATTATCCGAAA+TGG | - | Chr4:28838417-28838436 | MsG0480019873.01.T01:CDS | 35.0% | |
| GGATAATAGACACATGTTGT+TGG | + | Chr4:28838413-28838432 | None:intergenic | 35.0% | |
| TGTCTATTATCCGAAATGGT+TGG | - | Chr4:28838421-28838440 | MsG0480019873.01.T01:CDS | 35.0% | |
| ! | ATGAGCTTTTGTTCAACAGA+TGG | - | Chr4:28838377-28838396 | MsG0480019873.01.T01:CDS | 35.0% |
| ! | ATGTAACAAGTTTTGCCAAC+AGG | + | Chr4:28838290-28838309 | None:intergenic | 35.0% |
| ! | GATTTTGCATCTATTCCTGT+TGG | - | Chr4:28838272-28838291 | MsG0480019873.01.T01:CDS | 35.0% |
| !! | AGATGACAAGTACTGGATAT+GGG | - | Chr4:28838249-28838268 | MsG0480019873.01.T01:CDS | 35.0% |
| ATCATGCAGATGACAAGTAC+TGG | - | Chr4:28838242-28838261 | MsG0480019873.01.T01:CDS | 40.0% | |
| !! | ATGTGATGGATGTTTGGAGT+GGG | - | Chr4:28838136-28838155 | MsG0480019873.01.T01:CDS | 40.0% |
| !! | CAGATGACAAGTACTGGATA+TGG | - | Chr4:28838248-28838267 | MsG0480019873.01.T01:CDS | 40.0% |
| !! | GATGACAAGTACTGGATATG+GGG | - | Chr4:28838250-28838269 | MsG0480019873.01.T01:CDS | 40.0% |
| !! | GATGTTTGGAGTGGGTTAAA+AGG | - | Chr4:28838144-28838163 | MsG0480019873.01.T01:CDS | 40.0% |
| !! | TTCCACATGTGATGGATGTT+TGG | - | Chr4:28838130-28838149 | MsG0480019873.01.T01:CDS | 40.0% |
| CTCCAAACATCCATCACATG+TGG | + | Chr4:28838135-28838154 | None:intergenic | 45.0% | |
| GAGAGAGTTTCCACATGTGA+TGG | - | Chr4:28838122-28838141 | MsG0480019873.01.T01:CDS | 45.0% | |
| GTCTTCACAACTCAAGTCAC+TGG | - | Chr4:28838173-28838192 | MsG0480019873.01.T01:CDS | 45.0% | |
| !! | CATGTGATGGATGTTTGGAG+TGG | - | Chr4:28838135-28838154 | MsG0480019873.01.T01:CDS | 45.0% |
| !! | TGGAGTGGGTTAAAAGGAGT+GGG | - | Chr4:28838150-28838169 | MsG0480019873.01.T01:CDS | 45.0% |
| !! | TTGGAGTGGGTTAAAAGGAG+TGG | - | Chr4:28838149-28838168 | MsG0480019873.01.T01:CDS | 45.0% |
| !! | GGAGTGGGTTAAAAGGAGTG+GGG | - | Chr4:28838151-28838170 | MsG0480019873.01.T01:CDS | 50.0% |
| GGGGGGGGAATGCATTTCAT+TGG | - | Chr4:28838100-28838119 | MsG0480019873.01.T01:CDS | 55.0% | |
| ! | ATGTGGGGGGGGGGGGGGGG+GGG | - | Chr4:28838083-28838102 | MsG0480019873.01.T01:CDS | 85.0% |
| ! | TGTGGGGGGGGGGGGGGGGG+GGG | - | Chr4:28838084-28838103 | MsG0480019873.01.T01:CDS | 90.0% |
| ! | GTGGGGGGGGGGGGGGGGGG+GGG | - | Chr4:28838085-28838104 | MsG0480019873.01.T01:CDS | 95.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 28838083 | 28838451 | 28838083 | ID=MsG0480019873.01;Name=MsG0480019873.01 |
| Chr4 | mRNA | 28838083 | 28838451 | 28838083 | ID=MsG0480019873.01.T01;Parent=MsG0480019873.01;Name=MsG0480019873.01.T01;_AED=0.04;_eAED=0.04;_QI=0|-1|0|1|-1|0|1|0|122 |
| Chr4 | exon | 28838083 | 28838451 | 28838083 | ID=MsG0480019873.01.T01:exon:12076;Parent=MsG0480019873.01.T01 |
| Chr4 | CDS | 28838083 | 28838451 | 28838083 | ID=MsG0480019873.01.T01:cds;Parent=MsG0480019873.01.T01 |
| Gene Sequence |
| Protein sequence |
