Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680031154.01.T01 | KEH25281.1 | 76.724 | 232 | 51 | 1 | 1 | 232 | 163 | 391 | 4.24E-110 | 333 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680031154.01.T01 | Q9CZV8 | 28.947 | 228 | 151 | 6 | 12 | 233 | 125 | 347 | 4.03E-15 | 77 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680031154.01.T01 | A0A072U7E7 | 76.724 | 232 | 51 | 1 | 1 | 232 | 163 | 391 | 2.02e-110 | 333 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0280011032.01 | MsG0680031154.01 | 0.810797 | 9.638453e-51 | 5.865983e-48 |
| MsG0680031154.01 | MsG0680032801.01 | 0.806552 | 7.783443e-50 | 4.237047e-47 |
| MsG0680031154.01 | MsG0780037592.01 | 0.801396 | 9.184603e-49 | 4.381449e-46 |
| MsG0680031154.01 | MsG0880041836.01 | 0.804123 | 2.512181e-49 | 1.284867e-46 |
| MsG0680031154.01 | MsG0880042161.01 | 0.808622 | 2.829490e-50 | 1.625514e-47 |
| MsG0680031154.01 | MsG0880044574.01 | 0.801420 | 9.082240e-49 | 4.335315e-46 |
| MsG0480019874.01 | MsG0680031154.01 | 0.802364 | 5.809444e-49 | 2.840559e-46 |
| MsG0480022259.01 | MsG0680031154.01 | 0.816795 | 4.599285e-52 | 3.290907e-49 |
| MsG0480022262.01 | MsG0680031154.01 | 0.812224 | 4.721484e-51 | 2.984914e-48 |
| MsG0380015720.01 | MsG0680031154.01 | 0.800613 | 1.327499e-48 | 6.208727e-46 |
| MsG0380016475.01 | MsG0680031154.01 | 0.808279 | 3.348545e-50 | 1.906731e-47 |
| MsG0580027767.01 | MsG0680031154.01 | 0.810131 | 1.342246e-50 | 8.025918e-48 |
| MsG0580027922.01 | MsG0680031154.01 | 0.815899 | 7.297774e-52 | 5.095132e-49 |
| MsG0680030667.01 | MsG0680031154.01 | 0.812923 | 3.320175e-51 | 2.138093e-48 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680031154.01.T01 | MTR_6g016910 | 76.724 | 232 | 51 | 1 | 1 | 232 | 163 | 391 | 5.13e-114 | 333 |
| MsG0680031154.01.T01 | MTR_6g016705 | 61.333 | 225 | 79 | 2 | 15 | 232 | 159 | 382 | 1.10e-82 | 253 |
| MsG0680031154.01.T01 | MTR_6g025290 | 60.538 | 223 | 81 | 1 | 10 | 232 | 182 | 397 | 3.19e-81 | 249 |
| MsG0680031154.01.T01 | MTR_1g050478 | 64.319 | 213 | 73 | 2 | 20 | 232 | 325 | 534 | 3.22e-81 | 253 |
| MsG0680031154.01.T01 | MTR_5g046730 | 60.889 | 225 | 85 | 2 | 8 | 232 | 189 | 410 | 4.52e-81 | 249 |
| MsG0680031154.01.T01 | MTR_6g022820 | 62.385 | 218 | 81 | 1 | 15 | 232 | 228 | 444 | 2.58e-80 | 248 |
| MsG0680031154.01.T01 | MTR_4g133640 | 62.617 | 214 | 77 | 3 | 20 | 232 | 326 | 537 | 4.65e-75 | 238 |
| MsG0680031154.01.T01 | MTR_3g110740 | 60.094 | 213 | 82 | 2 | 20 | 232 | 344 | 553 | 1.05e-74 | 236 |
| MsG0680031154.01.T01 | MTR_2g007590 | 61.538 | 221 | 81 | 4 | 13 | 232 | 320 | 537 | 1.76e-74 | 236 |
| MsG0680031154.01.T01 | MTR_6g042905 | 57.456 | 228 | 93 | 3 | 5 | 232 | 169 | 392 | 4.11e-72 | 226 |
| MsG0680031154.01.T01 | MTR_5g075860 | 61.215 | 214 | 80 | 3 | 20 | 232 | 295 | 506 | 1.37e-71 | 228 |
| MsG0680031154.01.T01 | MTR_3g006050 | 61.860 | 215 | 80 | 2 | 19 | 232 | 327 | 540 | 1.60e-71 | 229 |
| MsG0680031154.01.T01 | MTR_6g016725 | 59.813 | 214 | 81 | 4 | 20 | 232 | 380 | 589 | 1.80e-69 | 224 |
| MsG0680031154.01.T01 | MTR_6g016905 | 55.455 | 220 | 93 | 3 | 14 | 232 | 310 | 525 | 2.68e-69 | 222 |
| MsG0680031154.01.T01 | MTR_6g016685 | 54.585 | 229 | 95 | 3 | 4 | 232 | 101 | 320 | 6.61e-69 | 215 |
| MsG0680031154.01.T01 | MTR_5g095830 | 58.879 | 214 | 85 | 3 | 20 | 232 | 336 | 547 | 8.24e-68 | 220 |
| MsG0680031154.01.T01 | MTR_4g006240 | 60.748 | 214 | 82 | 2 | 20 | 232 | 304 | 516 | 1.63e-67 | 218 |
| MsG0680031154.01.T01 | MTR_8g045150 | 58.216 | 213 | 74 | 2 | 20 | 232 | 239 | 436 | 1.02e-66 | 214 |
| MsG0680031154.01.T01 | MTR_6g016750 | 52.889 | 225 | 98 | 2 | 8 | 232 | 180 | 396 | 2.42e-66 | 211 |
| MsG0680031154.01.T01 | MTR_1g115380 | 56.744 | 215 | 88 | 4 | 20 | 232 | 296 | 507 | 9.32e-66 | 213 |
| MsG0680031154.01.T01 | MTR_7g044870 | 54.222 | 225 | 100 | 3 | 9 | 232 | 147 | 369 | 1.39e-65 | 208 |
| MsG0680031154.01.T01 | MTR_7g029535 | 59.346 | 214 | 82 | 4 | 20 | 232 | 378 | 587 | 1.32e-64 | 211 |
| MsG0680031154.01.T01 | MTR_3g036270 | 61.682 | 214 | 77 | 4 | 20 | 232 | 380 | 589 | 6.22e-64 | 209 |
| MsG0680031154.01.T01 | MTR_1g069090 | 51.724 | 232 | 79 | 5 | 7 | 232 | 28 | 232 | 2.19e-63 | 199 |
| MsG0680031154.01.T01 | MTR_6g016740 | 51.376 | 218 | 88 | 2 | 15 | 232 | 151 | 350 | 2.43e-63 | 202 |
| MsG0680031154.01.T01 | MTR_1g069145 | 49.782 | 229 | 89 | 3 | 6 | 231 | 256 | 461 | 4.41e-62 | 202 |
| MsG0680031154.01.T01 | MTR_1g115390 | 53.456 | 217 | 92 | 4 | 20 | 232 | 134 | 345 | 5.44e-62 | 197 |
| MsG0680031154.01.T01 | MTR_4g006450 | 53.425 | 219 | 81 | 2 | 19 | 232 | 299 | 501 | 6.51e-62 | 203 |
| MsG0680031154.01.T01 | MTR_5g088250 | 55.155 | 194 | 83 | 3 | 41 | 232 | 296 | 487 | 8.44e-60 | 197 |
| MsG0680031154.01.T01 | MTR_5g430490 | 53.052 | 213 | 99 | 1 | 20 | 232 | 315 | 526 | 1.43e-57 | 192 |
| MsG0680031154.01.T01 | MTR_3g083280 | 49.763 | 211 | 101 | 1 | 20 | 230 | 246 | 451 | 1.56e-56 | 187 |
| MsG0680031154.01.T01 | MTR_3g465770 | 50.472 | 212 | 100 | 2 | 20 | 230 | 314 | 521 | 4.65e-56 | 188 |
| MsG0680031154.01.T01 | MTR_1g075040 | 63.750 | 160 | 56 | 2 | 12 | 170 | 205 | 363 | 5.12e-56 | 182 |
| MsG0680031154.01.T01 | MTR_6g016915 | 48.131 | 214 | 109 | 2 | 19 | 232 | 148 | 359 | 4.92e-55 | 181 |
| MsG0680031154.01.T01 | MTR_4g059340 | 59.659 | 176 | 68 | 2 | 19 | 194 | 325 | 497 | 1.15e-54 | 183 |
| MsG0680031154.01.T01 | MTR_5g095810 | 55.897 | 195 | 62 | 3 | 20 | 213 | 58 | 229 | 3.66e-53 | 173 |
| MsG0680031154.01.T01 | MTR_8g017160 | 49.115 | 226 | 104 | 5 | 8 | 232 | 322 | 537 | 1.17e-52 | 179 |
| MsG0680031154.01.T01 | MTR_8g037780 | 49.115 | 226 | 103 | 6 | 8 | 232 | 317 | 531 | 2.46e-51 | 176 |
| MsG0680031154.01.T01 | MTR_6g054950 | 50.239 | 209 | 52 | 2 | 24 | 232 | 185 | 341 | 4.35e-49 | 165 |
| MsG0680031154.01.T01 | MTR_3g110148 | 61.268 | 142 | 54 | 1 | 91 | 232 | 182 | 322 | 6.72e-49 | 165 |
| MsG0680031154.01.T01 | MTR_4g009030 | 45.833 | 216 | 110 | 4 | 20 | 232 | 300 | 511 | 2.69e-48 | 167 |
| MsG0680031154.01.T01 | MTR_3g074570 | 48.241 | 199 | 97 | 2 | 33 | 230 | 206 | 399 | 9.70e-48 | 163 |
| MsG0680031154.01.T01 | MTR_1g069120 | 55.705 | 149 | 64 | 1 | 16 | 162 | 266 | 414 | 1.32e-46 | 160 |
| MsG0680031154.01.T01 | MTR_4g024200 | 44.286 | 210 | 115 | 2 | 21 | 229 | 204 | 412 | 1.66e-46 | 160 |
| MsG0680031154.01.T01 | MTR_4g019320 | 47.393 | 211 | 90 | 3 | 20 | 229 | 307 | 497 | 2.92e-46 | 161 |
| MsG0680031154.01.T01 | MTR_4g006210 | 56.322 | 174 | 66 | 5 | 47 | 213 | 164 | 334 | 3.46e-46 | 160 |
| MsG0680031154.01.T01 | MTR_6g016760 | 55.245 | 143 | 62 | 1 | 88 | 230 | 1 | 141 | 1.13e-43 | 147 |
| MsG0680031154.01.T01 | MTR_4g021765 | 41.589 | 214 | 121 | 4 | 21 | 232 | 66 | 277 | 4.55e-43 | 148 |
| MsG0680031154.01.T01 | MTR_6g027420 | 46.977 | 215 | 109 | 5 | 20 | 232 | 210 | 421 | 4.58e-43 | 151 |
| MsG0680031154.01.T01 | MTR_4g006290 | 46.190 | 210 | 88 | 6 | 25 | 232 | 207 | 393 | 5.82e-42 | 148 |
| MsG0680031154.01.T01 | MTR_6g016720 | 48.824 | 170 | 81 | 3 | 24 | 191 | 52 | 217 | 9.44e-39 | 134 |
| MsG0680031154.01.T01 | MTR_4g008430 | 56.291 | 151 | 64 | 2 | 20 | 169 | 154 | 303 | 4.73e-37 | 132 |
| MsG0680031154.01.T01 | MTR_2g078550 | 59.524 | 126 | 47 | 4 | 93 | 217 | 1 | 123 | 2.20e-36 | 125 |
| MsG0680031154.01.T01 | MTR_0295s0010 | 60.656 | 122 | 46 | 2 | 109 | 228 | 4 | 125 | 2.97e-36 | 129 |
| MsG0680031154.01.T01 | MTR_8g446780 | 45.078 | 193 | 98 | 2 | 40 | 231 | 27 | 212 | 9.22e-36 | 127 |
| MsG0680031154.01.T01 | MTR_4g435338 | 57.664 | 137 | 53 | 2 | 97 | 232 | 131 | 263 | 1.52e-35 | 128 |
| MsG0680031154.01.T01 | MTR_8g076010 | 43.627 | 204 | 95 | 4 | 47 | 232 | 182 | 383 | 1.68e-35 | 130 |
| MsG0680031154.01.T01 | MTR_5g087540 | 45.503 | 189 | 73 | 3 | 47 | 232 | 24 | 185 | 3.49e-35 | 125 |
| MsG0680031154.01.T01 | MTR_2g104970 | 59.048 | 105 | 42 | 1 | 129 | 232 | 8 | 112 | 1.39e-33 | 119 |
| MsG0680031154.01.T01 | MTR_1g023530 | 39.316 | 234 | 102 | 5 | 15 | 232 | 187 | 396 | 2.67e-33 | 125 |
| MsG0680031154.01.T01 | MTR_6g016900 | 69.412 | 85 | 26 | 0 | 150 | 234 | 24 | 108 | 3.14e-33 | 120 |
| MsG0680031154.01.T01 | MTR_3g081430 | 48.322 | 149 | 70 | 3 | 21 | 169 | 133 | 274 | 1.30e-31 | 117 |
| MsG0680031154.01.T01 | MTR_4g008420 | 53.509 | 114 | 51 | 2 | 120 | 232 | 134 | 246 | 2.29e-31 | 117 |
| MsG0680031154.01.T01 | MTR_3g110150 | 41.111 | 180 | 101 | 2 | 54 | 232 | 58 | 233 | 9.39e-31 | 115 |
| MsG0680031154.01.T01 | MTR_4g008450 | 44.949 | 198 | 96 | 5 | 41 | 232 | 52 | 242 | 1.07e-30 | 115 |
| MsG0680031154.01.T01 | MTR_3g073390 | 39.151 | 212 | 102 | 3 | 20 | 230 | 279 | 464 | 1.63e-30 | 118 |
| MsG0680031154.01.T01 | MTR_4g005810 | 56.818 | 132 | 55 | 2 | 102 | 232 | 11 | 141 | 1.71e-30 | 114 |
| MsG0680031154.01.T01 | MTR_4g021835 | 39.810 | 211 | 100 | 4 | 47 | 231 | 194 | 403 | 3.61e-29 | 114 |
| MsG0680031154.01.T01 | MTR_4g008600 | 50.000 | 136 | 61 | 1 | 20 | 155 | 95 | 223 | 3.17e-28 | 108 |
| MsG0680031154.01.T01 | MTR_7g100890 | 38.028 | 213 | 128 | 4 | 20 | 229 | 168 | 379 | 4.35e-28 | 111 |
| MsG0680031154.01.T01 | MTR_4g008400 | 54.808 | 104 | 45 | 2 | 130 | 232 | 138 | 240 | 8.59e-28 | 107 |
| MsG0680031154.01.T01 | MTR_4g008340 | 53.333 | 105 | 47 | 2 | 129 | 232 | 165 | 268 | 1.67e-27 | 107 |
| MsG0680031154.01.T01 | MTR_4g024220 | 57.407 | 108 | 45 | 1 | 125 | 231 | 37 | 144 | 5.68e-27 | 103 |
| MsG0680031154.01.T01 | MTR_4g008330 | 53.922 | 102 | 46 | 1 | 132 | 232 | 1 | 102 | 3.59e-26 | 100 |
| MsG0680031154.01.T01 | MTR_4g006280 | 49.123 | 114 | 57 | 1 | 119 | 231 | 171 | 284 | 7.65e-26 | 102 |
| MsG0680031154.01.T01 | MTR_8g060730 | 37.387 | 222 | 106 | 8 | 18 | 232 | 116 | 311 | 9.51e-26 | 103 |
| MsG0680031154.01.T01 | MTR_8g067230 | 41.117 | 197 | 101 | 7 | 42 | 232 | 70 | 257 | 1.64e-25 | 102 |
| MsG0680031154.01.T01 | MTR_3g081260 | 40.351 | 171 | 86 | 4 | 20 | 177 | 47 | 214 | 3.19e-25 | 100 |
| MsG0680031154.01.T01 | MTR_4g006250 | 59.524 | 84 | 34 | 0 | 149 | 232 | 9 | 92 | 3.35e-25 | 97.1 |
| MsG0680031154.01.T01 | MTR_3g074000 | 46.269 | 134 | 66 | 3 | 20 | 151 | 307 | 436 | 7.64e-25 | 102 |
| MsG0680031154.01.T01 | MTR_6g080560 | 47.154 | 123 | 61 | 2 | 114 | 232 | 126 | 248 | 2.12e-24 | 98.6 |
| MsG0680031154.01.T01 | MTR_4g008440 | 50.450 | 111 | 53 | 2 | 114 | 223 | 123 | 232 | 2.16e-24 | 97.4 |
| MsG0680031154.01.T01 | MTR_1g028400 | 38.710 | 186 | 81 | 4 | 47 | 232 | 284 | 436 | 7.47e-24 | 99.4 |
| MsG0680031154.01.T01 | MTR_4g008490 | 61.538 | 91 | 31 | 4 | 146 | 233 | 24 | 113 | 1.43e-23 | 93.6 |
| MsG0680031154.01.T01 | MTR_4g132730 | 71.212 | 66 | 18 | 1 | 167 | 232 | 143 | 207 | 7.04e-22 | 92.4 |
| MsG0680031154.01.T01 | MTR_3g074550 | 37.736 | 159 | 84 | 3 | 20 | 177 | 207 | 351 | 4.59e-21 | 90.9 |
| MsG0680031154.01.T01 | MTR_3g074550 | 30.729 | 192 | 105 | 6 | 51 | 230 | 155 | 330 | 2.17e-12 | 65.9 |
| MsG0680031154.01.T01 | MTR_5g067480 | 51.613 | 93 | 44 | 1 | 93 | 185 | 80 | 171 | 2.51e-19 | 84.0 |
| MsG0680031154.01.T01 | MTR_4g129140 | 32.075 | 212 | 89 | 5 | 21 | 232 | 257 | 413 | 2.54e-19 | 86.3 |
| MsG0680031154.01.T01 | MTR_4g008480 | 42.975 | 121 | 62 | 4 | 57 | 173 | 123 | 240 | 1.70e-18 | 82.4 |
| MsG0680031154.01.T01 | MTR_6g033270 | 48.913 | 92 | 29 | 1 | 10 | 101 | 190 | 263 | 2.23e-18 | 82.0 |
| MsG0680031154.01.T01 | MTR_6g018570 | 71.429 | 63 | 18 | 0 | 170 | 232 | 376 | 438 | 3.84e-18 | 83.2 |
| MsG0680031154.01.T01 | MTR_4g012820 | 63.934 | 61 | 21 | 1 | 142 | 201 | 125 | 185 | 2.07e-16 | 75.1 |
| MsG0680031154.01.T01 | MTR_5g067490 | 61.194 | 67 | 24 | 1 | 19 | 83 | 286 | 352 | 2.30e-15 | 74.7 |
| MsG0680031154.01.T01 | MTR_3g008790 | 34.848 | 132 | 56 | 1 | 101 | 232 | 38 | 139 | 3.74e-15 | 71.2 |
| MsG0680031154.01.T01 | MTR_4g006460 | 44.554 | 101 | 35 | 3 | 134 | 232 | 119 | 200 | 5.81e-15 | 72.4 |
| MsG0680031154.01.T01 | MTR_0041s0060 | 62.222 | 45 | 17 | 0 | 188 | 232 | 1 | 45 | 6.27e-14 | 64.7 |
| MsG0680031154.01.T01 | MTR_4g009055 | 36.943 | 157 | 77 | 6 | 19 | 170 | 212 | 351 | 2.50e-13 | 68.9 |
| MsG0680031154.01.T01 | MTR_4g008380 | 70.213 | 47 | 14 | 0 | 186 | 232 | 6 | 52 | 3.38e-13 | 64.3 |
| MsG0680031154.01.T01 | MTR_0807s0010 | 64.706 | 51 | 17 | 1 | 181 | 230 | 1 | 51 | 1.57e-12 | 62.0 |
| MsG0680031154.01.T01 | MTR_8g045170 | 38.235 | 102 | 59 | 3 | 57 | 155 | 221 | 321 | 6.92e-11 | 61.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680031154.01.T01 | AT1G80630 | 38.249 | 217 | 127 | 5 | 18 | 232 | 282 | 493 | 5.25e-30 | 117 |
Find 51 sgRNAs with CRISPR-Local
Find 52 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAACACGTCCACCAACTTTA+TGG | 0.157378 | 6:+15209182 | MsG0680031154.01.T01:CDS |
| AAAGAATTGTCCTGGGCTTT+TGG | 0.219466 | 6:+15209770 | MsG0680031154.01.T01:CDS |
| ATTAGGAATTTGAATTTAAC+TGG | 0.242510 | 6:+15209642 | MsG0680031154.01.T01:CDS |
| ACAACATTTGGATCTTTATC+TGG | 0.243205 | 6:+15209242 | MsG0680031154.01.T01:CDS |
| CAGCTCCAAATTGGTGAAAA+TGG | 0.270901 | 6:-15209553 | None:intergenic |
| CCAGCATGTTTCACTCCTTC+TGG | 0.297111 | 6:-15209825 | None:intergenic |
| AAAGCAATAACTAAGGTTAA+TGG | 0.309572 | 6:-15209325 | None:intergenic |
| GAAAGATGAAAACATCATAT+TGG | 0.331386 | 6:+15209524 | MsG0680031154.01.T01:CDS |
| GAGTTGTCTTTGTTTCTTGC+TGG | 0.341756 | 6:+15209294 | MsG0680031154.01.T01:CDS |
| GAAATCAAGCAGCTCCAAAT+TGG | 0.362610 | 6:-15209562 | None:intergenic |
| TAAGGAGATGTCGTAAGATT+AGG | 0.362611 | 6:+15209625 | MsG0680031154.01.T01:CDS |
| TTTACTCAGTGATGTCAAAA+TGG | 0.366863 | 6:+15209392 | MsG0680031154.01.T01:CDS |
| TCAATTGAAGTCTCTCTGTT+TGG | 0.372247 | 6:+15209485 | MsG0680031154.01.T01:CDS |
| GTCATCTCAAAGAATTGTCC+TGG | 0.396649 | 6:+15209762 | MsG0680031154.01.T01:CDS |
| TTCCATTACCCTCCTACTCC+TGG | 0.412468 | 6:+15209153 | MsG0680031154.01.T01:CDS |
| AAACTTTGAAGTTCCTAAGT+TGG | 0.412950 | 6:+15209692 | MsG0680031154.01.T01:CDS |
| AGGGAGTTCTAATTCTTTGA+AGG | 0.427638 | 6:+15209443 | MsG0680031154.01.T01:CDS |
| AAGTTCAACACCTCCAACTT+AGG | 0.443612 | 6:-15209705 | None:intergenic |
| GTGTCAATATATACAACATT+TGG | 0.444516 | 6:+15209230 | MsG0680031154.01.T01:CDS |
| CTTTGAAGTTCCTAAGTTGG+AGG | 0.458453 | 6:+15209695 | MsG0680031154.01.T01:CDS |
| AAAATGGAACACACAGGTAT+CGG | 0.475049 | 6:+15209408 | MsG0680031154.01.T01:CDS |
| TGTTCAAGAGTGAATCTACT+TGG | 0.476727 | 6:+15209666 | MsG0680031154.01.T01:CDS |
| AACAGAGAGACTTCAATTGA+GGG | 0.499788 | 6:-15209482 | None:intergenic |
| AAATGGAACACACAGGTATC+GGG | 0.508714 | 6:+15209409 | MsG0680031154.01.T01:CDS |
| AAACAGAGAGACTTCAATTG+AGG | 0.510285 | 6:-15209483 | None:intergenic |
| CTCCAGGAGTAGGAGGGTAA+TGG | 0.518411 | 6:-15209155 | None:intergenic |
| TGAGATCAAAGCAATAACTA+AGG | 0.526975 | 6:-15209332 | None:intergenic |
| TCATCTCAAAGAATTGTCCT+GGG | 0.532446 | 6:+15209763 | MsG0680031154.01.T01:CDS |
| CCAGAAGGAGTGAAACATGC+TGG | 0.532608 | 6:+15209825 | MsG0680031154.01.T01:CDS |
| AGTAGGAGGGTAATGGAACA+TGG | 0.534127 | 6:-15209148 | None:intergenic |
| CATAAAGTTGGTGGACGTGT+TGG | 0.535425 | 6:-15209181 | None:intergenic |
| GTGGACGTGTTGGTTACTCC+AGG | 0.542670 | 6:-15209171 | None:intergenic |
| ACGTCCACCAACTTTATGGA+TGG | 0.548223 | 6:+15209186 | MsG0680031154.01.T01:CDS |
| GTACATTCCATCCATAAAGT+TGG | 0.548360 | 6:-15209193 | None:intergenic |
| AGTAATAGTTCCAAAAGCCC+AGG | 0.549870 | 6:-15209780 | None:intergenic |
| TTGGTTACTCCAGGAGTAGG+AGG | 0.553845 | 6:-15209162 | None:intergenic |
| GGTATCGGGAAAGTGAGTGC+AGG | 0.568680 | 6:+15209423 | MsG0680031154.01.T01:CDS |
| TTGGAGCTGCTTGATTTCAG+TGG | 0.573789 | 6:+15209567 | MsG0680031154.01.T01:CDS |
| GTTGAACTTGTCATATACAA+AGG | 0.585440 | 6:+15209719 | MsG0680031154.01.T01:CDS |
| CATTCCATCCATAAAGTTGG+TGG | 0.588833 | 6:-15209190 | None:intergenic |
| ATGTTGTATATATTGACACT+TGG | 0.592360 | 6:-15209226 | None:intergenic |
| GTTCAAGAGTGAATCTACTT+GGG | 0.608380 | 6:+15209667 | MsG0680031154.01.T01:CDS |
| AGCAAGAAACAAAGACAACT+CGG | 0.612345 | 6:-15209292 | None:intergenic |
| GTGTTGGTTACTCCAGGAGT+AGG | 0.615840 | 6:-15209165 | None:intergenic |
| GGTTGCGATCAAATATCTCA+AGG | 0.616808 | 6:+15209588 | MsG0680031154.01.T01:CDS |
| CAGAAGGAGTGAAACATGCT+GGG | 0.617004 | 6:+15209826 | MsG0680031154.01.T01:CDS |
| GTATCGGGAAAGTGAGTGCA+GGG | 0.618679 | 6:+15209424 | MsG0680031154.01.T01:CDS |
| TGTCAAGATATCACACCAGA+AGG | 0.638726 | 6:+15209810 | MsG0680031154.01.T01:CDS |
| GTAGGAGGGTAATGGAACAT+GGG | 0.669148 | 6:-15209147 | None:intergenic |
| TGGTTACTCCAGGAGTAGGA+GGG | 0.679686 | 6:-15209161 | None:intergenic |
| GATGTCAAAATGGAACACAC+AGG | 0.710756 | 6:+15209402 | MsG0680031154.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTAGGAATTTGAATTTAAC+TGG | + | Chr6:15209642-15209661 | MsG0680031154.01.T01:CDS | 20.0% |
| ! | AAAGCAATAACTAAGGTTAA+TGG | - | Chr6:15209328-15209347 | None:intergenic | 25.0% |
| ! | ATGTTGTATATATTGACACT+TGG | - | Chr6:15209229-15209248 | None:intergenic | 25.0% |
| ! | GAAAGATGAAAACATCATAT+TGG | + | Chr6:15209524-15209543 | MsG0680031154.01.T01:CDS | 25.0% |
| ! | GTGTCAATATATACAACATT+TGG | + | Chr6:15209230-15209249 | MsG0680031154.01.T01:CDS | 25.0% |
| !!! | AAGGTATTTGTCAAGTTTTA+AGG | + | Chr6:15209607-15209626 | MsG0680031154.01.T01:CDS | 25.0% |
| ACAACATTTGGATCTTTATC+TGG | + | Chr6:15209242-15209261 | MsG0680031154.01.T01:CDS | 30.0% | |
| GTTGAACTTGTCATATACAA+AGG | + | Chr6:15209719-15209738 | MsG0680031154.01.T01:CDS | 30.0% | |
| TGAGATCAAAGCAATAACTA+AGG | - | Chr6:15209335-15209354 | None:intergenic | 30.0% | |
| TTTACTCAGTGATGTCAAAA+TGG | + | Chr6:15209392-15209411 | MsG0680031154.01.T01:CDS | 30.0% | |
| ! | TTCTTCCATTTTCACCAATT+TGG | + | Chr6:15209548-15209567 | MsG0680031154.01.T01:CDS | 30.0% |
| !! | AAACTTTGAAGTTCCTAAGT+TGG | + | Chr6:15209692-15209711 | MsG0680031154.01.T01:CDS | 30.0% |
| !! | ATTTTGACATCACTGAGTAA+AGG | - | Chr6:15209393-15209412 | None:intergenic | 30.0% |
| AAAATGGAACACACAGGTAT+CGG | + | Chr6:15209408-15209427 | MsG0680031154.01.T01:CDS | 35.0% | |
| AAACAGAGAGACTTCAATTG+AGG | - | Chr6:15209486-15209505 | None:intergenic | 35.0% | |
| AACAGAGAGACTTCAATTGA+GGG | - | Chr6:15209485-15209504 | None:intergenic | 35.0% | |
| AGCAAGAAACAAAGACAACT+CGG | - | Chr6:15209295-15209314 | None:intergenic | 35.0% | |
| GTACATTCCATCCATAAAGT+TGG | - | Chr6:15209196-15209215 | None:intergenic | 35.0% | |
| GTTCAAGAGTGAATCTACTT+GGG | + | Chr6:15209667-15209686 | MsG0680031154.01.T01:CDS | 35.0% | |
| TAAGGAGATGTCGTAAGATT+AGG | + | Chr6:15209625-15209644 | MsG0680031154.01.T01:CDS | 35.0% | |
| TCAATTGAAGTCTCTCTGTT+TGG | + | Chr6:15209485-15209504 | MsG0680031154.01.T01:CDS | 35.0% | |
| TCATCTCAAAGAATTGTCCT+GGG | + | Chr6:15209763-15209782 | MsG0680031154.01.T01:CDS | 35.0% | |
| TGTTCAAGAGTGAATCTACT+TGG | + | Chr6:15209666-15209685 | MsG0680031154.01.T01:CDS | 35.0% | |
| ! | AGGGAGTTCTAATTCTTTGA+AGG | + | Chr6:15209443-15209462 | MsG0680031154.01.T01:CDS | 35.0% |
| AAATGGAACACACAGGTATC+GGG | + | Chr6:15209409-15209428 | MsG0680031154.01.T01:CDS | 40.0% | |
| AAGTTCAACACCTCCAACTT+AGG | - | Chr6:15209708-15209727 | None:intergenic | 40.0% | |
| AGTAATAGTTCCAAAAGCCC+AGG | - | Chr6:15209783-15209802 | None:intergenic | 40.0% | |
| CAGCTCCAAATTGGTGAAAA+TGG | - | Chr6:15209556-15209575 | None:intergenic | 40.0% | |
| CATTCCATCCATAAAGTTGG+TGG | - | Chr6:15209193-15209212 | None:intergenic | 40.0% | |
| GAAATCAAGCAGCTCCAAAT+TGG | - | Chr6:15209565-15209584 | None:intergenic | 40.0% | |
| GATGTCAAAATGGAACACAC+AGG | + | Chr6:15209402-15209421 | MsG0680031154.01.T01:CDS | 40.0% | |
| GGTTGCGATCAAATATCTCA+AGG | + | Chr6:15209588-15209607 | MsG0680031154.01.T01:CDS | 40.0% | |
| GTCATCTCAAAGAATTGTCC+TGG | + | Chr6:15209762-15209781 | MsG0680031154.01.T01:CDS | 40.0% | |
| TGTCAAGATATCACACCAGA+AGG | + | Chr6:15209810-15209829 | MsG0680031154.01.T01:CDS | 40.0% | |
| ! | AAAGAATTGTCCTGGGCTTT+TGG | + | Chr6:15209770-15209789 | MsG0680031154.01.T01:CDS | 40.0% |
| ! | GAGTTGTCTTTGTTTCTTGC+TGG | + | Chr6:15209294-15209313 | MsG0680031154.01.T01:CDS | 40.0% |
| !! | CTTTGAAGTTCCTAAGTTGG+AGG | + | Chr6:15209695-15209714 | MsG0680031154.01.T01:CDS | 40.0% |
| ACGTCCACCAACTTTATGGA+TGG | + | Chr6:15209186-15209205 | MsG0680031154.01.T01:CDS | 45.0% | |
| CAACACGTCCACCAACTTTA+TGG | + | Chr6:15209182-15209201 | MsG0680031154.01.T01:CDS | 45.0% | |
| CAGAAGGAGTGAAACATGCT+GGG | + | Chr6:15209826-15209845 | MsG0680031154.01.T01:CDS | 45.0% | |
| TTGGAGCTGCTTGATTTCAG+TGG | + | Chr6:15209567-15209586 | MsG0680031154.01.T01:CDS | 45.0% | |
| !! | CATAAAGTTGGTGGACGTGT+TGG | - | Chr6:15209184-15209203 | None:intergenic | 45.0% |
| CCAGAAGGAGTGAAACATGC+TGG | + | Chr6:15209825-15209844 | MsG0680031154.01.T01:CDS | 50.0% | |
| CCAGCATGTTTCACTCCTTC+TGG | - | Chr6:15209828-15209847 | None:intergenic | 50.0% | |
| GTATCGGGAAAGTGAGTGCA+GGG | + | Chr6:15209424-15209443 | MsG0680031154.01.T01:CDS | 50.0% | |
| TGGTTACTCCAGGAGTAGGA+GGG | - | Chr6:15209164-15209183 | None:intergenic | 50.0% | |
| TTCCATTACCCTCCTACTCC+TGG | + | Chr6:15209153-15209172 | MsG0680031154.01.T01:CDS | 50.0% | |
| TTGGTTACTCCAGGAGTAGG+AGG | - | Chr6:15209165-15209184 | None:intergenic | 50.0% | |
| !! | GTGTTGGTTACTCCAGGAGT+AGG | - | Chr6:15209168-15209187 | None:intergenic | 50.0% |
| CTCCAGGAGTAGGAGGGTAA+TGG | - | Chr6:15209158-15209177 | None:intergenic | 55.0% | |
| ! | GGTATCGGGAAAGTGAGTGC+AGG | + | Chr6:15209423-15209442 | MsG0680031154.01.T01:CDS | 55.0% |
| !! | GTGGACGTGTTGGTTACTCC+AGG | - | Chr6:15209174-15209193 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr6 | gene | 15209150 | 15209854 | 15209150 | ID=MsG0680031154.01;Name=MsG0680031154.01 |
| Chr6 | mRNA | 15209150 | 15209854 | 15209150 | ID=MsG0680031154.01.T01;Parent=MsG0680031154.01;Name=MsG0680031154.01.T01;_AED=0.41;_eAED=0.41;_QI=0|-1|0|1|-1|1|1|0|234 |
| Chr6 | exon | 15209150 | 15209854 | 15209150 | ID=MsG0680031154.01.T01:exon:10237;Parent=MsG0680031154.01.T01 |
| Chr6 | CDS | 15209150 | 15209854 | 15209150 | ID=MsG0680031154.01.T01:cds;Parent=MsG0680031154.01.T01 |
| Gene Sequence |
| Protein sequence |
