AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480020401.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480020401.01.T01 MTR_4g050170 95.973 447 13 2 1 442 1 447 0.0 876
MsG0480020401.01.T01 MTR_7g106760 75.787 413 92 6 32 442 27 433 0.0 553
MsG0480020401.01.T01 MTR_2g087810 72.263 411 102 5 34 442 26 426 0.0 551
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480020401.01.T01 AT5G06390 70.824 449 111 5 3 442 1 438 0.0 575
MsG0480020401.01.T01 AT2G35860 75.980 408 87 4 36 442 33 430 0.0 570
MsG0480020401.01.T01 AT3G52370 74.510 408 88 4 36 442 82 474 0.0 560
MsG0480020401.01.T01 AT3G52370 74.510 408 88 4 36 442 29 421 0.0 556
MsG0480020401.01.T01 AT3G11700 69.623 451 118 6 3 442 1 443 0.0 546
MsG0480020401.01.T01 AT5G05650 58.696 92 34 1 320 411 2 89 3.95e-32 119

Find 125 sgRNAs with CRISPR-Local

Find 156 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGGGTTTACCCTTCCGTTCT+TGG 0.196546 4:-40626324 None:intergenic
TATAATGCTGTTAGAAGATT+TGG 0.205388 4:+40626097 MsG0480020401.01.T01:CDS
CTCCCATCCGTATAAATATC+TGG 0.225583 4:-40626226 None:intergenic
ATTCAAACACTGATACATTA+TGG 0.268025 4:+40625860 MsG0480020401.01.T01:CDS
ATCAACCTCCGGTGCACCTT+CGG 0.276459 4:-40625446 None:intergenic
GCCATAGATTGCGGCATCTA+TGG 0.285569 4:+40624842 MsG0480020401.01.T01:CDS
TCGCAACAATGGTTGGTTAA+TGG 0.287229 4:+40625286 MsG0480020401.01.T01:CDS
AGAGATGAATGATTGTTAAA+TGG 0.291033 4:-40624660 None:intergenic
TAACGAGATGGCGGATATTC+TGG 0.301192 4:+40625889 MsG0480020401.01.T01:CDS
ACCATAGATGCCGCAATCTA+TGG 0.306861 4:-40624843 None:intergenic
TGATTAAGAGGAGTTTGTTT+AGG 0.340226 4:-40624613 None:intergenic
GTTTGACCCAGATATTTATA+CGG 0.345120 4:+40626219 MsG0480020401.01.T01:CDS
GCTGATGGGTCGGTTAAATT+CGG 0.358905 4:+40626172 MsG0480020401.01.T01:CDS
TTAGCCATAGCTTCATCATT+TGG 0.365438 4:-40625983 None:intergenic
ATTGGCTCCTGGACCTTCTC+TGG 0.374989 4:+40625546 MsG0480020401.01.T01:CDS
TGAGCATCAGTTATTGATAC+AGG 0.376900 4:+40625252 MsG0480020401.01.T01:CDS
TCAACCTCCGGTGCACCTTC+GGG 0.377012 4:-40625445 None:intergenic
CAACGCACCTCTTCTTTGCT+TGG 0.384525 4:-40626359 None:intergenic
CCATTACCTGGTGCTGGCGC+CGG 0.386857 4:-40625571 None:intergenic
AAAAGAAGAGGTTGTTGATA+AGG 0.389174 4:+40626294 MsG0480020401.01.T01:CDS
CCGGGTCGACCCGCCTTTGT+TGG 0.396403 4:-40625212 None:intergenic
GGGTTTACCCTTCCGTTCTT+GGG 0.397284 4:-40626323 None:intergenic
CCGCCACCACCGCAGAAGCC+TGG 0.404968 4:+40625493 MsG0480020401.01.T01:CDS
GACCCCACCATCATTTCAAC+GGG 0.406272 4:+40625816 MsG0480020401.01.T01:CDS
ATTTCTGTGCAAGGGATTGA+TGG 0.408807 4:+40626250 MsG0480020401.01.T01:CDS
TGATAGTACATAATTTGTTC+CGG 0.408846 4:-40626040 None:intergenic
TACTTTGAGATTGCCTCATA+AGG 0.412712 4:+40626135 MsG0480020401.01.T01:CDS
GTGAACCCGGTTCACTCAAC+TGG 0.414386 4:-40626018 None:intergenic
ATAAACACAGAGGTAGCATT+TGG 0.415473 4:+40624775 MsG0480020401.01.T01:five_prime_UTR
TCTTCAGCATCAACCTTATG+AGG 0.417041 4:-40626148 None:intergenic
TTCATCTTCTTCTTCTTCAA+CGG 0.419684 4:+40624919 MsG0480020401.01.T01:CDS
ATAATGCTGTTAGAAGATTT+GGG 0.425540 4:+40626098 MsG0480020401.01.T01:CDS
AAACTCAAACTCAATCTTAG+TGG 0.429019 4:+40624949 MsG0480020401.01.T01:CDS
AACGGACCAGTTGAGTGAAC+CGG 0.429059 4:+40626012 MsG0480020401.01.T01:CDS
TGCTTGGCCACAACCTTAGC+GGG 0.430153 4:-40626343 None:intergenic
GGAAGAGCAGGCGGAGAGCC+AGG 0.439938 4:-40625511 None:intergenic
CTGGCACCGGCGCCAGCACC+AGG 0.447448 4:+40625565 MsG0480020401.01.T01:CDS
AGACCTGATGGTGTTATTCA+TGG 0.447511 4:+40625340 MsG0480020401.01.T01:CDS
CTCTCTTCAGTTTGATACTC+AGG 0.448810 4:-40626070 None:intergenic
CAAGATCTCGTTCAAGAGCT+TGG 0.451144 4:-40625087 None:intergenic
GTTAAATTCGGGCATGGTGA+TGG 0.463040 4:+40626184 MsG0480020401.01.T01:CDS
CCAGGCTTCTGCGGTGGTGG+CGG 0.467825 4:-40625493 None:intergenic
GATGAGTAGGTTAGTATCAG+AGG 0.468619 4:+40625937 MsG0480020401.01.T01:CDS
TCCTGGACCTTCTCTGGCAC+CGG 0.471775 4:+40625552 MsG0480020401.01.T01:CDS
GAGCCAGGCTTCTGCGGTGG+TGG 0.479008 4:-40625496 None:intergenic
CCAACAAAGGCGGGTCGACC+CGG 0.482239 4:+40625212 MsG0480020401.01.T01:CDS
TTGTTAATTTGGTGATTAAG+AGG 0.484592 4:-40624625 None:intergenic
CTGATGGGTCGGTTAAATTC+GGG 0.490096 4:+40626173 MsG0480020401.01.T01:CDS
ATTCCATGAATAACACCATC+AGG 0.490128 4:-40625343 None:intergenic
TTTCTGTGCAAGGGATTGAT+GGG 0.500163 4:+40626251 MsG0480020401.01.T01:CDS
GACCCAGATATTTATACGGA+TGG 0.504898 4:+40626223 MsG0480020401.01.T01:CDS
TTGAAAGGCTTCTTATTCCA+AGG 0.507843 4:+40625365 MsG0480020401.01.T01:CDS
AAGCTTCCATTACCTGGTGC+TGG 0.507955 4:-40625577 None:intergenic
CAACGGGGAGGCACAGGTGA+AGG 0.509640 4:+40625832 MsG0480020401.01.T01:CDS
CAAACGACAATGTTCTATGC+CGG 0.510547 4:-40625231 None:intergenic
TAAGATTCCGTGGCTCTAAG+AGG 0.512177 4:-40625129 None:intergenic
GGGTAAACCCGCTAAGGTTG+TGG 0.515104 4:+40626336 MsG0480020401.01.T01:CDS
GGCGGAGAGCCAGGCTTCTG+CGG 0.518421 4:-40625502 None:intergenic
GTGTTATTCATGGAATTGAA+AGG 0.521049 4:+40625350 MsG0480020401.01.T01:CDS
AGCACCAAATGATGAAGCTA+TGG 0.522967 4:+40625979 MsG0480020401.01.T01:CDS
AAATGATGGTGGGGTCCACC+AGG 0.523892 4:-40625809 None:intergenic
ACCCAGATATTTATACGGAT+GGG 0.523918 4:+40626224 MsG0480020401.01.T01:CDS
GCTGGCGCCGGTGCCAGAGA+AGG 0.527163 4:-40625559 None:intergenic
TCCCATCCGTATAAATATCT+GGG 0.527187 4:-40626225 None:intergenic
ATAATTTGTTCCGGTGAACC+CGG 0.528069 4:-40626031 None:intergenic
TTGCTTGGCCACAACCTTAG+CGG 0.531460 4:-40626344 None:intergenic
ATGAGTAGGTTAGTATCAGA+GGG 0.533753 4:+40625938 MsG0480020401.01.T01:CDS
GTGCCTCCCCGTTGAAATGA+TGG 0.534564 4:-40625823 None:intergenic
GTTGAGTGAACCGGGTTCAC+CGG 0.536161 4:+40626021 MsG0480020401.01.T01:CDS
GGCTGTGACCCAAGAACGGA+AGG 0.537283 4:+40626315 MsG0480020401.01.T01:CDS
TGTGTCCTCGGATCAACCTC+CGG 0.537319 4:-40625457 None:intergenic
AAGTCATCTTGAACAGACCT+TGG 0.542381 4:-40625382 None:intergenic
AAACGACAATGTTCTATGCC+GGG 0.550630 4:-40625230 None:intergenic
TGAAACATCGCAACAATGGT+TGG 0.551947 4:+40625279 MsG0480020401.01.T01:CDS
CTCCCCGTTGAAATGATGGT+GGG 0.553409 4:-40625819 None:intergenic
TCATTTCAACGGGGAGGCAC+AGG 0.554998 4:+40625826 MsG0480020401.01.T01:CDS
TTAGAAGATTTGGGAAGGTG+AGG 0.560046 4:+40626107 MsG0480020401.01.T01:CDS
GCCGGTGCCAGAGAAGGTCC+AGG 0.566022 4:-40625553 None:intergenic
TTCTGTGCAAGGGATTGATG+GGG 0.568281 4:+40626252 MsG0480020401.01.T01:CDS
GGACCCCACCATCATTTCAA+CGG 0.574499 4:+40625815 MsG0480020401.01.T01:CDS
ACGGACCAGTTGAGTGAACC+GGG 0.578352 4:+40626013 MsG0480020401.01.T01:CDS
AATTAAACCCGAAGGTGCAC+CGG 0.579641 4:+40625438 MsG0480020401.01.T01:CDS
ATGGGAGGATTTCTGTGCAA+GGG 0.579676 4:+40626242 MsG0480020401.01.T01:CDS
TGCTGTTAGAAGATTTGGGA+AGG 0.581041 4:+40626102 MsG0480020401.01.T01:CDS
ACGGAAGGGTAAACCCGCTA+AGG 0.584103 4:+40626330 MsG0480020401.01.T01:CDS
GAGCATCAGTTATTGATACA+GGG 0.589102 4:+40625253 MsG0480020401.01.T01:CDS
GAACTTAACGTCGTTAGCGA+CGG 0.595166 4:+40625913 MsG0480020401.01.T01:CDS
AAACTTTCCTCTTAGAGCCA+CGG 0.597093 4:+40625122 MsG0480020401.01.T01:CDS
CCTCCCCGTTGAAATGATGG+TGG 0.597615 4:-40625820 None:intergenic
GAAATGAAACATCGCAACAA+TGG 0.598171 4:+40625275 MsG0480020401.01.T01:CDS
GATCTGTATCCAACAAAGGC+GGG 0.603752 4:+40625203 MsG0480020401.01.T01:CDS
GGGTCGGTTAAATTCGGGCA+TGG 0.604050 4:+40626178 MsG0480020401.01.T01:CDS
GGAGAGCCAGGCTTCTGCGG+TGG 0.606784 4:-40625499 None:intergenic
ATTGCTGCAATTAAACCCGA+AGG 0.616715 4:+40625430 MsG0480020401.01.T01:CDS
TTATGGAGGTTATAACGAGA+TGG 0.617430 4:+40625877 MsG0480020401.01.T01:CDS
ATCTGGGTCAAACAAATAAG+CGG 0.617447 4:-40626209 None:intergenic
CGTTAGCGACGGAGATGAGT+AGG 0.618054 4:+40625924 MsG0480020401.01.T01:CDS
GATGGGAGGATTTCTGTGCA+AGG 0.618422 4:+40626241 MsG0480020401.01.T01:CDS
TGAAGAGATTTAAGATTCCG+TGG 0.620500 4:-40625139 None:intergenic
ATTATTCCTACACGTATCAA+CGG 0.621998 4:+40625181 MsG0480020401.01.T01:CDS
TCCCCGTTGAAATGATGGTG+GGG 0.631232 4:-40625818 None:intergenic
CAGATATTTATACGGATGGG+AGG 0.632435 4:+40626227 MsG0480020401.01.T01:CDS
ATAAGGCTGTGACCCAAGAA+CGG 0.632729 4:+40626311 MsG0480020401.01.T01:CDS
GTTGATGCTGAAGAAGCTGA+TGG 0.639771 4:+40626157 MsG0480020401.01.T01:CDS
AAGATGACTTTAACCGTCGT+AGG 0.641102 4:+40625395 MsG0480020401.01.T01:CDS
TAGAAACAAGACTCCTACGA+CGG 0.647653 4:-40625408 None:intergenic
GGATCTGTATCCAACAAAGG+CGG 0.658563 4:+40625202 MsG0480020401.01.T01:CDS
AATGATGTGATGAGACCTGA+TGG 0.663714 4:+40625328 MsG0480020401.01.T01:CDS
TAAACCCGAAGGTGCACCGG+AGG 0.664246 4:+40625441 MsG0480020401.01.T01:CDS
TGCTGAAGAAGCTGATGGGT+CGG 0.664468 4:+40626162 MsG0480020401.01.T01:CDS
AACGGATCTGTATCCAACAA+AGG 0.664692 4:+40625199 MsG0480020401.01.T01:CDS
GAATGAAGAAGAAGAACGTG+CGG 0.665760 4:-40624883 None:intergenic
GTTGTGGCCAAGCAAAGAAG+AGG 0.666635 4:+40626352 MsG0480020401.01.T01:CDS
ACAGATCCGTTGATACGTGT+AGG 0.669477 4:-40625187 None:intergenic
TTGATGCTGAAGAAGCTGAT+GGG 0.675714 4:+40626158 MsG0480020401.01.T01:CDS
CAAACACTGATACATTATGG+AGG 0.675816 4:+40625863 MsG0480020401.01.T01:CDS
CCACCATCATTTCAACGGGG+AGG 0.679312 4:+40625820 MsG0480020401.01.T01:CDS
TGGAGGTTATAACGAGATGG+CGG 0.695354 4:+40625880 MsG0480020401.01.T01:CDS
CAAACACTAGAAGACACTGT+CGG 0.700543 4:+40625031 MsG0480020401.01.T01:CDS
AGGTTGATCCGAGGACACAT+CGG 0.701711 4:+40625461 MsG0480020401.01.T01:CDS
AGCTATGGCTAAGTTAACAA+CGG 0.711535 4:+40625994 MsG0480020401.01.T01:CDS
GTGCACCGGAGGTTGATCCG+AGG 0.717893 4:+40625452 MsG0480020401.01.T01:CDS
GCTGTGACCCAAGAACGGAA+GGG 0.741038 4:+40626316 MsG0480020401.01.T01:CDS
AAACATAAACATAAACACAG+AGG 0.748225 4:+40624765 MsG0480020401.01.T01:five_prime_UTR
ACCCCACCATCATTTCAACG+GGG 0.810320 4:+40625817 MsG0480020401.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAACCAAAACCTTAAAAAA+TGG + Chr4:40624820-40624839 MsG0480020401.01.T01:exon 20.0%
!! TTGAATATAATCAGATGTAT+TGG - Chr4:40625688-40625707 None:intergenic 20.0%
! AAACATAAACATAAACACAG+AGG + Chr4:40624765-40624784 MsG0480020401.01.T01:five_prime_UTR 25.0%
! AGAGATGAATGATTGTTAAA+TGG - Chr4:40624663-40624682 None:intergenic 25.0%
! ATAATGCTGTTAGAAGATTT+GGG + Chr4:40626098-40626117 MsG0480020401.01.T01:CDS 25.0%
! ATTCAAACACTGATACATTA+TGG + Chr4:40625860-40625879 MsG0480020401.01.T01:CDS 25.0%
! TATAATGCTGTTAGAAGATT+TGG + Chr4:40626097-40626116 MsG0480020401.01.T01:CDS 25.0%
!! TTGTTAATTTGGTGATTAAG+AGG - Chr4:40624628-40624647 None:intergenic 25.0%
!!! AAGAGGAAAGTTTTGAAATT+TGG - Chr4:40625115-40625134 None:intergenic 25.0%
!!! AGAGGAAAGTTTTGAAATTT+GGG - Chr4:40625114-40625133 None:intergenic 25.0%
!!! GTTTTGTTGTGTTGTTAATT+TGG - Chr4:40624639-40624658 None:intergenic 25.0%
!!! TGATAGTACATAATTTGTTC+CGG - Chr4:40626043-40626062 None:intergenic 25.0%
!!! TTGTAACTAACTTGTTTTTC+AGG + Chr4:40625785-40625804 MsG0480020401.01.T01:intron 25.0%
AAAAGAAGAGGTTGTTGATA+AGG + Chr4:40626294-40626313 MsG0480020401.01.T01:CDS 30.0%
AAACTCAAACTCAATCTTAG+TGG + Chr4:40624949-40624968 MsG0480020401.01.T01:CDS 30.0%
ATTATTCCTACACGTATCAA+CGG + Chr4:40625181-40625200 MsG0480020401.01.T01:CDS 30.0%
GTTTGACCCAGATATTTATA+CGG + Chr4:40626219-40626238 MsG0480020401.01.T01:CDS 30.0%
TAAAAAATGGCCATAGATTG+CGG + Chr4:40624833-40624852 MsG0480020401.01.T01:exon 30.0%
TTCATCTTCTTCTTCTTCAA+CGG + Chr4:40624919-40624938 MsG0480020401.01.T01:CDS 30.0%
! GTGTTATTCATGGAATTGAA+AGG + Chr4:40625350-40625369 MsG0480020401.01.T01:CDS 30.0%
!!! ATGGCCATTTTTTAAGGTTT+TGG - Chr4:40624827-40624846 None:intergenic 30.0%
!!! CAATCTATGGCCATTTTTTA+AGG - Chr4:40624833-40624852 None:intergenic 30.0%
!!! TCTTCCTATTTTTGATGCAT+TGG + Chr4:40625528-40625547 MsG0480020401.01.T01:CDS 30.0%
ACCCAGATATTTATACGGAT+GGG + Chr4:40626224-40626243 MsG0480020401.01.T01:CDS 35.0%
AGCTATGGCTAAGTTAACAA+CGG + Chr4:40625994-40626013 MsG0480020401.01.T01:CDS 35.0%
ATAAACACAGAGGTAGCATT+TGG + Chr4:40624775-40624794 MsG0480020401.01.T01:five_prime_UTR 35.0%
ATCTGGGTCAAACAAATAAG+CGG - Chr4:40626212-40626231 None:intergenic 35.0%
ATGAGTAGGTTAGTATCAGA+GGG + Chr4:40625938-40625957 MsG0480020401.01.T01:CDS 35.0%
ATTCCATGAATAACACCATC+AGG - Chr4:40625346-40625365 None:intergenic 35.0%
CAAACACTGATACATTATGG+AGG + Chr4:40625863-40625882 MsG0480020401.01.T01:CDS 35.0%
GAAATGAAACATCGCAACAA+TGG + Chr4:40625275-40625294 MsG0480020401.01.T01:CDS 35.0%
GAGCATCAGTTATTGATACA+GGG + Chr4:40625253-40625272 MsG0480020401.01.T01:CDS 35.0%
GTTAATAAGCTTCCATTACC+TGG - Chr4:40625586-40625605 None:intergenic 35.0%
TCCACATGAAGAAAAAGAAG+AGG + Chr4:40626282-40626301 MsG0480020401.01.T01:CDS 35.0%
TCCCATCCGTATAAATATCT+GGG - Chr4:40626228-40626247 None:intergenic 35.0%
TGAGCATCAGTTATTGATAC+AGG + Chr4:40625252-40625271 MsG0480020401.01.T01:CDS 35.0%
TTAGCCATAGCTTCATCATT+TGG - Chr4:40625986-40626005 None:intergenic 35.0%
TTATGGAGGTTATAACGAGA+TGG + Chr4:40625877-40625896 MsG0480020401.01.T01:CDS 35.0%
TTGAAAGGCTTCTTATTCCA+AGG + Chr4:40625365-40625384 MsG0480020401.01.T01:CDS 35.0%
! ACCTCTTCTTTTTCTTCATG+TGG - Chr4:40626286-40626305 None:intergenic 35.0%
! TAATCAGATGTATTGGTGTC+TGG - Chr4:40625681-40625700 None:intergenic 35.0%
! TGAAGAGATTTAAGATTCCG+TGG - Chr4:40625142-40625161 None:intergenic 35.0%
!! TACTTTGAGATTGCCTCATA+AGG + Chr4:40626135-40626154 MsG0480020401.01.T01:CDS 35.0%
AAACGACAATGTTCTATGCC+GGG - Chr4:40625233-40625252 None:intergenic 40.0%
AAACTTTCCTCTTAGAGCCA+CGG + Chr4:40625122-40625141 MsG0480020401.01.T01:CDS 40.0%
AACGGATCTGTATCCAACAA+AGG + Chr4:40625199-40625218 MsG0480020401.01.T01:CDS 40.0%
AAGATGACTTTAACCGTCGT+AGG + Chr4:40625395-40625414 MsG0480020401.01.T01:CDS 40.0%
AAGTCATCTTGAACAGACCT+TGG - Chr4:40625385-40625404 None:intergenic 40.0%
AATGATGTGATGAGACCTGA+TGG + Chr4:40625328-40625347 MsG0480020401.01.T01:CDS 40.0%
AGCACCAAATGATGAAGCTA+TGG + Chr4:40625979-40625998 MsG0480020401.01.T01:CDS 40.0%
ATTGCTGCAATTAAACCCGA+AGG + Chr4:40625430-40625449 MsG0480020401.01.T01:CDS 40.0%
ATTTCTGTGCAAGGGATTGA+TGG + Chr4:40626250-40626269 MsG0480020401.01.T01:CDS 40.0%
CAAACACTAGAAGACACTGT+CGG + Chr4:40625031-40625050 MsG0480020401.01.T01:CDS 40.0%
CAAACGACAATGTTCTATGC+CGG - Chr4:40625234-40625253 None:intergenic 40.0%
CAGATATTTATACGGATGGG+AGG + Chr4:40626227-40626246 MsG0480020401.01.T01:CDS 40.0%
CATGTCAATTGTGTAGTGTC+TGG + Chr4:40625740-40625759 MsG0480020401.01.T01:intron 40.0%
CTCCCATCCGTATAAATATC+TGG - Chr4:40626229-40626248 None:intergenic 40.0%
CTCTCTTCAGTTTGATACTC+AGG - Chr4:40626073-40626092 None:intergenic 40.0%
GAATGAAGAAGAAGAACGTG+CGG - Chr4:40624886-40624905 None:intergenic 40.0%
GACCCAGATATTTATACGGA+TGG + Chr4:40626223-40626242 MsG0480020401.01.T01:CDS 40.0%
GATGAGTAGGTTAGTATCAG+AGG + Chr4:40625937-40625956 MsG0480020401.01.T01:CDS 40.0%
GCATCAAAAATAGGAAGAGC+AGG - Chr4:40625526-40625545 None:intergenic 40.0%
GGAGCCAATGCATCAAAAAT+AGG - Chr4:40625535-40625554 None:intergenic 40.0%
TAGAAACAAGACTCCTACGA+CGG - Chr4:40625411-40625430 None:intergenic 40.0%
TCAAAAATAGGAAGAGCAGG+CGG - Chr4:40625523-40625542 None:intergenic 40.0%
TCTTCAGCATCAACCTTATG+AGG - Chr4:40626151-40626170 None:intergenic 40.0%
TGAAACATCGCAACAATGGT+TGG + Chr4:40625279-40625298 MsG0480020401.01.T01:CDS 40.0%
TGCTGTTAGAAGATTTGGGA+AGG + Chr4:40626102-40626121 MsG0480020401.01.T01:CDS 40.0%
TTAGAAGATTTGGGAAGGTG+AGG + Chr4:40626107-40626126 MsG0480020401.01.T01:CDS 40.0%
TTGATGCTGAAGAAGCTGAT+GGG + Chr4:40626158-40626177 MsG0480020401.01.T01:CDS 40.0%
TTTCTGTGCAAGGGATTGAT+GGG + Chr4:40626251-40626270 MsG0480020401.01.T01:CDS 40.0%
! ACAAATTAGTTTCGAGCACC+CGG - Chr4:40625769-40625788 None:intergenic 40.0%
! TCGCAACAATGGTTGGTTAA+TGG + Chr4:40625286-40625305 MsG0480020401.01.T01:CDS 40.0%
!! AGACCTGATGGTGTTATTCA+TGG + Chr4:40625340-40625359 MsG0480020401.01.T01:CDS 40.0%
!! ATAATTTGTTCCGGTGAACC+CGG - Chr4:40626034-40626053 None:intergenic 40.0%
!! TTTTTCAACCGATGTGTCCT+CGG - Chr4:40625472-40625491 None:intergenic 40.0%
!!! ATTTTTGATGCATTGGCTCC+TGG + Chr4:40625535-40625554 MsG0480020401.01.T01:CDS 40.0%
!!! CGTTCAAGAGCTTGGTTTTT+TGG - Chr4:40625082-40625101 None:intergenic 40.0%
!!! CTAACTTGTTTTTCAGGACC+TGG + Chr4:40625791-40625810 MsG0480020401.01.T01:intron 40.0%
ACAGATCCGTTGATACGTGT+AGG - Chr4:40625190-40625209 None:intergenic 45.0%
ACCATAGATGCCGCAATCTA+TGG - Chr4:40624846-40624865 None:intergenic 45.0%
ATAAGGCTGTGACCCAAGAA+CGG + Chr4:40626311-40626330 MsG0480020401.01.T01:CDS 45.0%
ATGGGAGGATTTCTGTGCAA+GGG + Chr4:40626242-40626261 MsG0480020401.01.T01:CDS 45.0%
ATTGTGTAGTGTCTGGTGTC+CGG + Chr4:40625747-40625766 MsG0480020401.01.T01:intron 45.0%
CAAGATCTCGTTCAAGAGCT+TGG - Chr4:40625090-40625109 None:intergenic 45.0%
GAACTTAACGTCGTTAGCGA+CGG + Chr4:40625913-40625932 MsG0480020401.01.T01:CDS 45.0%
GATCTGTATCCAACAAAGGC+GGG + Chr4:40625203-40625222 MsG0480020401.01.T01:CDS 45.0%
GGATCTGTATCCAACAAAGG+CGG + Chr4:40625202-40625221 MsG0480020401.01.T01:CDS 45.0%
GTTAAATTCGGGCATGGTGA+TGG + Chr4:40626184-40626203 MsG0480020401.01.T01:CDS 45.0%
GTTGATGCTGAAGAAGCTGA+TGG + Chr4:40626157-40626176 MsG0480020401.01.T01:CDS 45.0%
TGGAGGTTATAACGAGATGG+CGG + Chr4:40625880-40625899 MsG0480020401.01.T01:CDS 45.0%
TTCTGTGCAAGGGATTGATG+GGG + Chr4:40626252-40626271 MsG0480020401.01.T01:CDS 45.0%
! AATTAAACCCGAAGGTGCAC+CGG + Chr4:40625438-40625457 MsG0480020401.01.T01:CDS 45.0%
! ACGTCTTCCATCAGAAGTGT+CGG - Chr4:40625614-40625633 None:intergenic 45.0%
! ATGTATTGGTGTCTGGTGTC+AGG - Chr4:40625674-40625693 None:intergenic 45.0%
! CTGATGGGTCGGTTAAATTC+GGG + Chr4:40626173-40626192 MsG0480020401.01.T01:CDS 45.0%
! GCTGATGGGTCGGTTAAATT+CGG + Chr4:40626172-40626191 MsG0480020401.01.T01:CDS 45.0%
!! TAACGAGATGGCGGATATTC+TGG + Chr4:40625889-40625908 MsG0480020401.01.T01:CDS 45.0%
!! TAAGATTCCGTGGCTCTAAG+AGG - Chr4:40625132-40625151 None:intergenic 45.0%
!!! ACTTGTTTTTCAGGACCTGG+TGG + Chr4:40625794-40625813 MsG0480020401.01.T01:intron 45.0%
AACGGACCAGTTGAGTGAAC+CGG + Chr4:40626012-40626031 MsG0480020401.01.T01:CDS 50.0%
AAGCTTCCATTACCTGGTGC+TGG - Chr4:40625580-40625599 None:intergenic 50.0%
ACACTGACCGACACTTCTGA+TGG + Chr4:40625604-40625623 MsG0480020401.01.T01:intron 50.0%
ACCCCACCATCATTTCAACG+GGG + Chr4:40625817-40625836 MsG0480020401.01.T01:CDS 50.0%
CAACGCACCTCTTCTTTGCT+TGG - Chr4:40626362-40626381 None:intergenic 50.0%
CTCCCCGTTGAAATGATGGT+GGG - Chr4:40625822-40625841 None:intergenic 50.0%
CTTCTGATGGAAGACGTGTC+TGG + Chr4:40625617-40625636 MsG0480020401.01.T01:intron 50.0%
GACCCCACCATCATTTCAAC+GGG + Chr4:40625816-40625835 MsG0480020401.01.T01:CDS 50.0%
GATGGGAGGATTTCTGTGCA+AGG + Chr4:40626241-40626260 MsG0480020401.01.T01:CDS 50.0%
GCCATAGATTGCGGCATCTA+TGG + Chr4:40624842-40624861 MsG0480020401.01.T01:CDS 50.0%
GGACCCCACCATCATTTCAA+CGG + Chr4:40625815-40625834 MsG0480020401.01.T01:CDS 50.0%
GGGTTTACCCTTCCGTTCTT+GGG - Chr4:40626326-40626345 None:intergenic 50.0%
GTTGTGGCCAAGCAAAGAAG+AGG + Chr4:40626352-40626371 MsG0480020401.01.T01:CDS 50.0%
TTGCTTGGCCACAACCTTAG+CGG - Chr4:40626347-40626366 None:intergenic 50.0%
TTGTGTAGTGTCTGGTGTCC+GGG + Chr4:40625748-40625767 MsG0480020401.01.T01:intron 50.0%
! AGGTTGATCCGAGGACACAT+CGG + Chr4:40625461-40625480 MsG0480020401.01.T01:CDS 50.0%
! TCCCCGTTGAAATGATGGTG+GGG - Chr4:40625821-40625840 None:intergenic 50.0%
! TGCTGAAGAAGCTGATGGGT+CGG + Chr4:40626162-40626181 MsG0480020401.01.T01:CDS 50.0%
ACGGAAGGGTAAACCCGCTA+AGG + Chr4:40626330-40626349 MsG0480020401.01.T01:CDS 55.0%
ACGGACCAGTTGAGTGAACC+GGG + Chr4:40626013-40626032 MsG0480020401.01.T01:CDS 55.0%
ATCAACCTCCGGTGCACCTT+CGG - Chr4:40625449-40625468 None:intergenic 55.0%
CCACCATCATTTCAACGGGG+AGG + Chr4:40625820-40625839 MsG0480020401.01.T01:CDS 55.0%
CCTCCCCGTTGAAATGATGG+TGG - Chr4:40625823-40625842 None:intergenic 55.0%
CGGGTTTACCCTTCCGTTCT+TGG - Chr4:40626327-40626346 None:intergenic 55.0%
CGTTAGCGACGGAGATGAGT+AGG + Chr4:40625924-40625943 MsG0480020401.01.T01:CDS 55.0%
GCTGTGACCCAAGAACGGAA+GGG + Chr4:40626316-40626335 MsG0480020401.01.T01:CDS 55.0%
GGGTAAACCCGCTAAGGTTG+TGG + Chr4:40626336-40626355 MsG0480020401.01.T01:CDS 55.0%
GGGTCGGTTAAATTCGGGCA+TGG + Chr4:40626178-40626197 MsG0480020401.01.T01:CDS 55.0%
GTGAACCCGGTTCACTCAAC+TGG - Chr4:40626021-40626040 None:intergenic 55.0%
GTGCCTCCCCGTTGAAATGA+TGG - Chr4:40625826-40625845 None:intergenic 55.0%
GTTGAGTGAACCGGGTTCAC+CGG + Chr4:40626021-40626040 MsG0480020401.01.T01:CDS 55.0%
TGCTTGGCCACAACCTTAGC+GGG - Chr4:40626346-40626365 None:intergenic 55.0%
TGGAAGACGTGTCTGGTGTC+TGG + Chr4:40625624-40625643 MsG0480020401.01.T01:intron 55.0%
TGTGTCCTCGGATCAACCTC+CGG - Chr4:40625460-40625479 None:intergenic 55.0%
! ATTGGCTCCTGGACCTTCTC+TGG + Chr4:40625546-40625565 MsG0480020401.01.T01:CDS 55.0%
! TCATTTCAACGGGGAGGCAC+AGG + Chr4:40625826-40625845 MsG0480020401.01.T01:CDS 55.0%
!! AAATGATGGTGGGGTCCACC+AGG - Chr4:40625812-40625831 None:intergenic 55.0%
GGCTGTGACCCAAGAACGGA+AGG + Chr4:40626315-40626334 MsG0480020401.01.T01:CDS 60.0%
TCAACCTCCGGTGCACCTTC+GGG - Chr4:40625448-40625467 None:intergenic 60.0%
TCCTGGACCTTCTCTGGCAC+CGG + Chr4:40625552-40625571 MsG0480020401.01.T01:CDS 60.0%
! TAAACCCGAAGGTGCACCGG+AGG + Chr4:40625441-40625460 MsG0480020401.01.T01:CDS 60.0%
CCAACAAAGGCGGGTCGACC+CGG + Chr4:40625212-40625231 MsG0480020401.01.T01:CDS 65.0%
! CAACGGGGAGGCACAGGTGA+AGG + Chr4:40625832-40625851 MsG0480020401.01.T01:CDS 65.0%
! GTGCACCGGAGGTTGATCCG+AGG + Chr4:40625452-40625471 MsG0480020401.01.T01:CDS 65.0%
!! CCATTACCTGGTGCTGGCGC+CGG - Chr4:40625574-40625593 None:intergenic 65.0%
CCGGCGCCAGCACCAGGTAA+TGG + Chr4:40625571-40625590 MsG0480020401.01.T01:intron 70.0%
CCGGGTCGACCCGCCTTTGT+TGG - Chr4:40625215-40625234 None:intergenic 70.0%
GAGCCAGGCTTCTGCGGTGG+TGG - Chr4:40625499-40625518 None:intergenic 70.0%
GGAAGAGCAGGCGGAGAGCC+AGG - Chr4:40625514-40625533 None:intergenic 70.0%
GGAGAGCCAGGCTTCTGCGG+TGG - Chr4:40625502-40625521 None:intergenic 70.0%
GGCGGAGAGCCAGGCTTCTG+CGG - Chr4:40625505-40625524 None:intergenic 70.0%
! CCAGGCTTCTGCGGTGGTGG+CGG - Chr4:40625496-40625515 None:intergenic 70.0%
! GCCGGTGCCAGAGAAGGTCC+AGG - Chr4:40625556-40625575 None:intergenic 70.0%
CCGCCACCACCGCAGAAGCC+TGG + Chr4:40625493-40625512 MsG0480020401.01.T01:CDS 75.0%
!! GCTGGCGCCGGTGCCAGAGA+AGG - Chr4:40625562-40625581 None:intergenic 75.0%
!!! CTGGCACCGGCGCCAGCACC+AGG + Chr4:40625565-40625584 MsG0480020401.01.T01:CDS 80.0%
Chromosome Type Strat End Strand Name
Chr4 gene 40624616 40626387 40624616 ID=MsG0480020401.01;Name=MsG0480020401.01
Chr4 mRNA 40624616 40626387 40624616 ID=MsG0480020401.01.T01;Parent=MsG0480020401.01;Name=MsG0480020401.01.T01;_AED=0.35;_eAED=0.35;_QI=201|1|0.66|1|0.5|0.33|3|0|442
Chr4 exon 40624616 40624688 40624616 ID=MsG0480020401.01.T01:exon:5830;Parent=MsG0480020401.01.T01
Chr4 exon 40624711 40625586 40624711 ID=MsG0480020401.01.T01:exon:5831;Parent=MsG0480020401.01.T01
Chr4 exon 40625807 40626387 40625807 ID=MsG0480020401.01.T01:exon:5832;Parent=MsG0480020401.01.T01
Chr4 five_prime_UTR 40624616 40624688 40624616 ID=MsG0480020401.01.T01:five_prime_utr;Parent=MsG0480020401.01.T01
Chr4 five_prime_UTR 40624711 40624838 40624711 ID=MsG0480020401.01.T01:five_prime_utr;Parent=MsG0480020401.01.T01
Chr4 CDS 40624839 40625586 40624839 ID=MsG0480020401.01.T01:cds;Parent=MsG0480020401.01.T01
Chr4 CDS 40625807 40626387 40625807 ID=MsG0480020401.01.T01:cds;Parent=MsG0480020401.01.T01
Gene Sequence

>MsG0480020401.01.T01

ATGGCCATAGATTGCGGCATCTATGGTTTCATCAACATCAAACACCGCACGTTCTTCTTCTTCATTCTTCTTCTATCTTTTTCATCTTCTTCTTCTTCAACGGTTCAAATAAACTCAAACTCAATCTTAGTGGCTTTACTAGACTCACATTATACAGAACTAGCTGAACTCGTTGAAAAAGCTATGCTTCTACAAACACTAGAAGACACTGTCGGTAAAAACAACATCACAATCTTCGCACCAAAAAACCAAGCTCTTGAACGAGATCTTGACCCAAATTTCAAAACTTTCCTCTTAGAGCCACGGAATCTTAAATCTCTTCAGACTTTGTTAATGTCACATATTATTCCTACACGTATCAACGGATCTGTATCCAACAAAGGCGGGTCGACCCGGCATAGAACATTGTCGTTTGAGCATCAGTTATTGATACAGGGAAATGAAACATCGCAACAATGGTTGGTTAATGGAGCAAAGATTGTTCATCTGAATGATGTGATGAGACCTGATGGTGTTATTCATGGAATTGAAAGGCTTCTTATTCCAAGGTCTGTTCAAGATGACTTTAACCGTCGTAGGAGTCTTGTTTCTATTGCTGCAATTAAACCCGAAGGTGCACCGGAGGTTGATCCGAGGACACATCGGTTGAAAAAGCCGCCACCACCGCAGAAGCCTGGCTCTCCGCCTGCTCTTCCTATTTTTGATGCATTGGCTCCTGGACCTTCTCTGGCACCGGCGCCAGCACCAGGACCTGGTGGACCCCACCATCATTTCAACGGGGAGGCACAGGTGAAGGATTTTATTCAAACACTGATACATTATGGAGGTTATAACGAGATGGCGGATATTCTGGTGAACTTAACGTCGTTAGCGACGGAGATGAGTAGGTTAGTATCAGAGGGTTATGTTTTAACAGTTCTAGCACCAAATGATGAAGCTATGGCTAAGTTAACAACGGACCAGTTGAGTGAACCGGGTTCACCGGAACAAATTATGTACTATCATTTGATTCCTGAGTATCAAACTGAAGAGAGTATGTATAATGCTGTTAGAAGATTTGGGAAGGTGAGGTATGATACTTTGAGATTGCCTCATAAGGTTGATGCTGAAGAAGCTGATGGGTCGGTTAAATTCGGGCATGGTGATGGTTCCGCTTATTTGTTTGACCCAGATATTTATACGGATGGGAGGATTTCTGTGCAAGGGATTGATGGGGTTTTGTTTCCACATGAAGAAAAAGAAGAGGTTGTTGATAAGGCTGTGACCCAAGAACGGAAGGGTAAACCCGCTAAGGTTGTGGCCAAGCAAAGAAGAGGTGCGTTGCTTTGA
Protein sequence

>MsG0480020401.01.T01

MAIDCGIYGFINIKHRTFFFFILLLSFSSSSSSTVQINSNSILVALLDSHYTELAELVEKAMLLQTLEDTVGKNNITIFAPKNQALERDLDPNFKTFLLEPRNLKSLQTLLMSHIIPTRINGSVSNKGGSTRHRTLSFEHQLLIQGNETSQQWLVNGAKIVHLNDVMRPDGVIHGIERLLIPRSVQDDFNRRRSLVSIAAIKPEGAPEVDPRTHRLKKPPPPQKPGSPPALPIFDALAPGPSLAPAPAPGPGGPHHHFNGEAQVKDFIQTLIHYGGYNEMADILVNLTSLATEMSRLVSEGYVLTVLAPNDEAMAKLTTDQLSEPGSPEQIMYYHLIPEYQTEESMYNAVRRFGKVRYDTLRLPHKVDAEEADGSVKFGHGDGSAYLFDPDIYTDGRISVQGIDGVLFPHEEKEEVVDKAVTQERKGKPAKVVAKQRRGALL*