Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024109.01.T01 | XP_003610667.1 | 94.527 | 201 | 11 | 0 | 1 | 201 | 1 | 201 | 2.73E-137 | 392 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024109.01.T01 | Q84MC0 | 58.791 | 182 | 72 | 2 | 20 | 201 | 20 | 198 | 5.27E-70 | 214 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024109.01.T01 | B7FGN4 | 94.527 | 201 | 11 | 0 | 1 | 201 | 1 | 201 | 1.30e-137 | 392 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080047814.01 | MsG0580024109.01 | 0.809327 | 1.998539e-50 | 1.169970e-47 |
| MsG0080048701.01 | MsG0580024109.01 | 0.812086 | 5.059494e-51 | 3.186496e-48 |
| MsG0080049140.01 | MsG0580024109.01 | 0.821191 | 4.603732e-53 | 3.719960e-50 |
| MsG0180000275.01 | MsG0580024109.01 | 0.805951 | 1.041821e-49 | 5.584942e-47 |
| MsG0180000360.01 | MsG0580024109.01 | 0.845061 | 5.137109e-59 | 8.458178e-56 |
| MsG0180000445.01 | MsG0580024109.01 | 0.801644 | 8.169772e-49 | 3.921813e-46 |
| MsG0180000557.01 | MsG0580024109.01 | 0.829015 | 6.520031e-55 | 6.590093e-52 |
| MsG0180001198.01 | MsG0580024109.01 | 0.854410 | 1.253649e-61 | 2.797071e-58 |
| MsG0180001237.01 | MsG0580024109.01 | 0.812405 | 4.309604e-51 | 2.737909e-48 |
| MsG0180002421.01 | MsG0580024109.01 | 0.855209 | 7.356285e-62 | 1.685287e-58 |
| MsG0180003509.01 | MsG0580024109.01 | 0.800606 | 1.331834e-48 | 6.227882e-46 |
| MsG0180003820.01 | MsG0580024109.01 | 0.809793 | 1.587366e-50 | 9.408421e-48 |
| MsG0180003858.01 | MsG0580024109.01 | 0.832385 | 9.740632e-56 | 1.087016e-52 |
| MsG0180004126.01 | MsG0580024109.01 | 0.830615 | 2.658680e-55 | 2.815706e-52 |
| MsG0180004754.01 | MsG0580024109.01 | 0.855026 | 8.312518e-62 | 1.892968e-58 |
| MsG0180004989.01 | MsG0580024109.01 | 0.811922 | 5.494565e-51 | 3.444893e-48 |
| MsG0180005667.01 | MsG0580024109.01 | 0.805808 | 1.116115e-49 | 5.961492e-47 |
| MsG0180005835.01 | MsG0580024109.01 | 0.805931 | 1.051566e-49 | 5.634417e-47 |
| MsG0280010779.01 | MsG0580024109.01 | 0.844682 | 6.498984e-59 | 1.057291e-55 |
| MsG0280010795.01 | MsG0580024109.01 | 0.819323 | 1.233616e-52 | 9.461946e-50 |
| MsG0280010977.01 | MsG0580024109.01 | 0.863013 | 3.369942e-64 | 1.006727e-60 |
| MsG0280011154.01 | MsG0580024109.01 | 0.831997 | 1.215274e-55 | 1.340876e-52 |
| MsG0380014130.01 | MsG0580024109.01 | 0.801686 | 8.010946e-49 | 3.849598e-46 |
| MsG0380014132.01 | MsG0580024109.01 | 0.810692 | 1.015834e-50 | 6.165082e-48 |
| MsG0380014808.01 | MsG0580024109.01 | 0.819374 | 1.201238e-52 | 9.226552e-50 |
| MsG0480018526.01 | MsG0580024109.01 | 0.823009 | 1.744655e-53 | 1.484178e-50 |
| MsG0480018885.01 | MsG0580024109.01 | 0.803878 | 2.825552e-49 | 1.436111e-46 |
| MsG0480018918.01 | MsG0580024109.01 | 0.801459 | 8.915760e-49 | 4.260057e-46 |
| MsG0480018919.01 | MsG0580024109.01 | 0.800102 | 1.686859e-48 | 7.787753e-46 |
| MsG0480019635.01 | MsG0580024109.01 | 0.820138 | 8.035707e-53 | 6.306204e-50 |
| MsG0480020401.01 | MsG0580024109.01 | 0.801200 | 1.007299e-48 | 4.781734e-46 |
| MsG0480020495.01 | MsG0580024109.01 | 0.822148 | 2.766559e-53 | 2.296446e-50 |
| MsG0480020597.01 | MsG0580024109.01 | 0.872243 | 3.694115e-67 | 1.534734e-63 |
| MsG0480020847.01 | MsG0580024109.01 | 0.802349 | 5.852931e-49 | 2.860660e-46 |
| MsG0480020965.01 | MsG0580024109.01 | 0.810533 | 1.099346e-50 | 6.643283e-48 |
| MsG0480021013.01 | MsG0580024109.01 | 0.815630 | 8.378021e-52 | 5.807165e-49 |
| MsG0480021156.01 | MsG0580024109.01 | 0.835763 | 1.388271e-56 | 1.714305e-53 |
| MsG0480021225.01 | MsG0580024109.01 | 0.847618 | 1.031743e-59 | 1.844406e-56 |
| MsG0480022167.01 | MsG0580024109.01 | 0.827873 | 1.229669e-54 | 1.202272e-51 |
| MsG0480022325.01 | MsG0580024109.01 | 0.862836 | 3.821608e-64 | 1.134904e-60 |
| MsG0480022331.01 | MsG0580024109.01 | 0.846287 | 2.387423e-59 | 4.088921e-56 |
| MsG0480022620.01 | MsG0580024109.01 | 0.810792 | 9.666883e-51 | 5.882417e-48 |
| MsG0480022952.01 | MsG0580024109.01 | 0.840058 | 1.092735e-57 | 1.538527e-54 |
| MsG0480022998.01 | MsG0580024109.01 | 0.800785 | 1.224298e-48 | 5.750876e-46 |
| MsG0480023053.01 | MsG0580024109.01 | 0.831748 | 1.400420e-55 | 1.533900e-52 |
| MsG0480023296.01 | MsG0580024109.01 | 0.833987 | 3.887652e-56 | 4.551923e-53 |
| MsG0480023324.01 | MsG0580024109.01 | 0.808170 | 3.532803e-50 | 2.005893e-47 |
| MsG0480023450.01 | MsG0580024109.01 | 0.823826 | 1.123591e-53 | 9.781914e-51 |
| MsG0480023620.01 | MsG0580024109.01 | 0.846153 | 2.596923e-59 | 4.428496e-56 |
| MsG0480023647.01 | MsG0580024109.01 | 0.800189 | 1.619401e-48 | 7.492794e-46 |
| MsG0480023672.01 | MsG0580024109.01 | 0.838801 | 2.317427e-57 | 3.138542e-54 |
| MsG0480023737.01 | MsG0580024109.01 | 0.825635 | 4.210444e-54 | 3.859355e-51 |
| MsG0480023755.01 | MsG0580024109.01 | 0.815217 | 1.035452e-51 | 7.095126e-49 |
| MsG0480023874.01 | MsG0580024109.01 | 0.804740 | 1.868145e-49 | 9.708423e-47 |
| MsG0580024109.01 | MsG0580024478.01 | 0.824718 | 6.932788e-54 | 6.191386e-51 |
| MsG0580024109.01 | MsG0580024489.01 | 0.854894 | 9.078020e-62 | 2.058171e-58 |
| MsG0580024109.01 | MsG0580024722.01 | 0.805471 | 1.313489e-49 | 6.954959e-47 |
| MsG0580024109.01 | MsG0580024758.01 | 0.804248 | 2.366120e-49 | 1.213979e-46 |
| MsG0580024109.01 | MsG0580024798.01 | 0.831209 | 1.900387e-55 | 2.048211e-52 |
| MsG0580024109.01 | MsG0580024813.01 | 0.803135 | 4.027917e-49 | 2.008381e-46 |
| MsG0580024109.01 | MsG0580024954.01 | 0.805565 | 1.255448e-49 | 6.663489e-47 |
| MsG0580024109.01 | MsG0580025093.01 | 0.827901 | 1.210710e-54 | 1.184672e-51 |
| MsG0580024109.01 | MsG0580025352.01 | 0.861791 | 8.005415e-64 | 2.291814e-60 |
| MsG0580024109.01 | MsG0580025614.01 | 0.805587 | 1.242146e-49 | 6.596854e-47 |
| MsG0580024109.01 | MsG0580025766.01 | 0.843283 | 1.540953e-58 | 2.399365e-55 |
| MsG0580024109.01 | MsG0580026059.01 | 0.823283 | 1.505801e-53 | 1.290855e-50 |
| MsG0580024109.01 | MsG0580026186.01 | 0.837230 | 5.877368e-57 | 7.585318e-54 |
| MsG0580024109.01 | MsG0580026593.01 | 0.804628 | 1.971377e-49 | 1.021424e-46 |
| MsG0580024109.01 | MsG0580028060.01 | 0.877618 | 5.437839e-69 | 2.751859e-65 |
| MsG0580024109.01 | MsG0580028330.01 | 0.826155 | 3.168596e-54 | 2.947703e-51 |
| MsG0580024109.01 | MsG0580028516.01 | 0.809450 | 1.880757e-50 | 1.104587e-47 |
| MsG0580024109.01 | MsG0580028756.01 | 0.835886 | 1.291981e-56 | 1.601271e-53 |
| MsG0580024109.01 | MsG0580029298.01 | 0.865233 | 6.851247e-65 | 2.212836e-61 |
| MsG0580024109.01 | MsG0680030865.01 | 0.811645 | 6.312776e-51 | 3.928762e-48 |
| MsG0580024109.01 | MsG0680031616.01 | 0.811274 | 7.598247e-51 | 4.682935e-48 |
| MsG0580024109.01 | MsG0680034598.01 | 0.818013 | 2.445746e-52 | 1.809400e-49 |
| MsG0580024109.01 | MsG0680034906.01 | 0.801446 | 8.969038e-49 | 4.284180e-46 |
| MsG0580024109.01 | MsG0780036752.01 | 0.861043 | 1.353156e-63 | 3.776495e-60 |
| MsG0580024109.01 | MsG0780038373.01 | 0.800576 | 1.350529e-48 | 6.310596e-46 |
| MsG0580024109.01 | MsG0780040139.01 | 0.811202 | 7.875824e-51 | 4.844889e-48 |
| MsG0580024109.01 | MsG0780040364.01 | 0.820897 | 5.379432e-53 | 4.311290e-50 |
| MsG0580024109.01 | MsG0780040472.01 | 0.822411 | 2.403861e-53 | 2.010581e-50 |
| MsG0580024109.01 | MsG0780040482.01 | 0.808537 | 2.950419e-50 | 1.691336e-47 |
| MsG0580024109.01 | MsG0780040541.01 | 0.812760 | 3.605441e-51 | 2.311853e-48 |
| MsG0580024109.01 | MsG0780040543.01 | 0.812347 | 4.438274e-51 | 2.815158e-48 |
| MsG0580024109.01 | MsG0780040564.01 | 0.833602 | 4.853173e-56 | 5.616562e-53 |
| MsG0580024109.01 | MsG0780040688.01 | 0.802117 | 6.532406e-49 | 3.173751e-46 |
| MsG0580024109.01 | MsG0780040828.01 | 0.817998 | 2.465203e-52 | 1.823073e-49 |
| MsG0580024109.01 | MsG0780041063.01 | 0.837539 | 4.897140e-57 | 6.381172e-54 |
| MsG0580024109.01 | MsG0780041078.01 | 0.806412 | 8.329215e-50 | 4.517850e-47 |
| MsG0580024109.01 | MsG0780041132.01 | 0.804646 | 1.954460e-49 | 1.013163e-46 |
| MsG0580024109.01 | MsG0780041143.01 | 0.815293 | 9.955575e-52 | 6.836659e-49 |
| MsG0580024109.01 | MsG0780041212.01 | 0.818166 | 2.258511e-52 | 1.677921e-49 |
| MsG0580024109.01 | MsG0780041363.01 | 0.806531 | 7.861426e-50 | 4.277129e-47 |
| MsG0580024109.01 | MsG0780041756.01 | 0.819141 | 1.357150e-52 | 1.035722e-49 |
| MsG0580024109.01 | MsG0780041804.01 | 0.812240 | 4.682321e-51 | 2.961395e-48 |
| MsG0580024109.01 | MsG0880041860.01 | 0.810721 | 1.001272e-50 | 6.081454e-48 |
| MsG0580024109.01 | MsG0880042552.01 | 0.802906 | 4.491786e-49 | 2.226737e-46 |
| MsG0580024109.01 | MsG0880042773.01 | 0.885268 | 9.416843e-72 | 6.421123e-68 |
| MsG0580024109.01 | MsG0880042791.01 | 0.841165 | 5.605917e-58 | 8.167051e-55 |
| MsG0580024109.01 | MsG0880042836.01 | 0.805959 | 1.037709e-49 | 5.564118e-47 |
| MsG0580024109.01 | MsG0880042845.01 | 0.842402 | 2.643455e-58 | 4.003907e-55 |
| MsG0580024109.01 | MsG0880042906.01 | 0.830075 | 3.601917e-55 | 3.755019e-52 |
| MsG0580024109.01 | MsG0880042929.01 | 0.813916 | 2.008726e-51 | 1.328868e-48 |
| MsG0580024109.01 | MsG0880043297.01 | 0.803372 | 3.598014e-49 | 1.804942e-46 |
| MsG0580024109.01 | MsG0880043516.01 | 0.839769 | 1.299339e-57 | 1.812635e-54 |
| MsG0580024109.01 | MsG0880043637.01 | 0.803233 | 3.844418e-49 | 1.921706e-46 |
| MsG0580024109.01 | MsG0880044803.01 | 0.816266 | 6.043456e-52 | 4.262367e-49 |
| MsG0580024109.01 | MsG0880045295.01 | 0.813754 | 2.180890e-51 | 1.436454e-48 |
| MsG0580024109.01 | MsG0880045430.01 | 0.849904 | 2.395899e-60 | 4.612199e-57 |
| MsG0580024109.01 | MsG0880045552.01 | 0.827965 | 1.168554e-54 | 1.145668e-51 |
| MsG0580024109.01 | MsG0880045890.01 | 0.810792 | 9.665683e-51 | 5.881747e-48 |
| MsG0580024109.01 | MsG0880046086.01 | 0.865253 | 6.754764e-65 | 2.183199e-61 |
| MsG0580024109.01 | MsG0880046885.01 | 0.813136 | 2.982601e-51 | 1.931673e-48 |
| MsG0580024109.01 | MsG0880047226.01 | 0.866915 | 2.011116e-65 | 6.893308e-62 |
| MsG0580024109.01 | MsG0880047293.01 | 0.829430 | 5.170322e-55 | 5.290513e-52 |
| MsG0580024109.01 | MsG0880047466.01 | 0.832336 | 1.001731e-55 | 1.116143e-52 |
| MsG0380015548.01 | MsG0580024109.01 | 0.890354 | 1.063040e-73 | 8.907613e-70 |
| MsG0380016140.01 | MsG0580024109.01 | 0.802497 | 5.454548e-49 | 2.675876e-46 |
| MsG0380016449.01 | MsG0580024109.01 | 0.821086 | 4.866797e-53 | 3.921111e-50 |
| MsG0380016703.01 | MsG0580024109.01 | 0.843680 | 1.207596e-58 | 1.903907e-55 |
| MsG0380016994.01 | MsG0580024109.01 | 0.847340 | 1.230110e-59 | 2.179367e-56 |
| MsG0380017159.01 | MsG0580024109.01 | 0.886735 | 2.640816e-72 | 1.909304e-68 |
| MsG0380017164.01 | MsG0580024109.01 | 0.809775 | 1.601527e-50 | 9.488106e-48 |
| MsG0380017240.01 | MsG0580024109.01 | 0.898338 | 5.854062e-77 | 6.888125e-73 |
| MsG0380017361.01 | MsG0580024109.01 | 0.804119 | 2.516663e-49 | 1.287042e-46 |
| MsG0380017559.01 | MsG0580024109.01 | 0.805692 | 1.180449e-49 | 6.286515e-47 |
| MsG0380017836.01 | MsG0580024109.01 | 0.804444 | 2.153565e-49 | 1.110544e-46 |
| MsG0380017983.01 | MsG0580024109.01 | 0.821727 | 3.461571e-53 | 2.839176e-50 |
| MsG0480018230.01 | MsG0580024109.01 | 0.823567 | 1.291981e-53 | 1.116485e-50 |
| MsG0480018256.01 | MsG0580024109.01 | 0.816063 | 6.705916e-52 | 4.703479e-49 |
| MsG0480018397.01 | MsG0580024109.01 | 0.812764 | 3.598342e-51 | 2.307524e-48 |
| MsG0280006477.01 | MsG0580024109.01 | 0.841464 | 4.677963e-58 | 6.879635e-55 |
| MsG0280006540.01 | MsG0580024109.01 | 0.804198 | 2.423999e-49 | 1.242097e-46 |
| MsG0280007359.01 | MsG0580024109.01 | 0.834704 | 2.569247e-56 | 3.072932e-53 |
| MsG0280007419.01 | MsG0580024109.01 | 0.846291 | 2.381770e-59 | 4.079846e-56 |
| MsG0280007761.01 | MsG0580024109.01 | 0.801628 | 8.232099e-49 | 3.950100e-46 |
| MsG0280007790.01 | MsG0580024109.01 | 0.800892 | 1.164411e-48 | 5.484941e-46 |
| MsG0280007983.01 | MsG0580024109.01 | 0.852336 | 4.936097e-61 | 1.028096e-57 |
| MsG0280008280.01 | MsG0580024109.01 | 0.818673 | 1.733490e-52 | 1.306039e-49 |
| MsG0280008614.01 | MsG0580024109.01 | 0.820451 | 6.812744e-53 | 5.392804e-50 |
| MsG0280008679.01 | MsG0580024109.01 | 0.806630 | 7.494020e-50 | 4.087245e-47 |
| MsG0280008909.01 | MsG0580024109.01 | 0.822954 | 1.796643e-53 | 1.525977e-50 |
| MsG0280009241.01 | MsG0580024109.01 | 0.809595 | 1.751118e-50 | 1.032474e-47 |
| MsG0280009514.01 | MsG0580024109.01 | 0.827830 | 1.259611e-54 | 1.229918e-51 |
| MsG0280009752.01 | MsG0580024109.01 | 0.805146 | 1.536498e-49 | 8.068342e-47 |
| MsG0280009794.01 | MsG0580024109.01 | 0.806906 | 6.552828e-50 | 3.599664e-47 |
| MsG0280010001.01 | MsG0580024109.01 | 0.819789 | 9.656479e-53 | 7.504438e-50 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024109.01.T01 | MTR_5g005710 | 94.527 | 201 | 11 | 0 | 1 | 201 | 1 | 201 | 3.30e-141 | 392 |
| MsG0580024109.01.T01 | MTR_1g105305 | 65.366 | 205 | 61 | 3 | 1 | 201 | 1 | 199 | 2.20e-94 | 273 |
| MsG0580024109.01.T01 | MTR_7g113640 | 40.000 | 160 | 92 | 3 | 14 | 170 | 16 | 174 | 1.51e-35 | 124 |
| MsG0580024109.01.T01 | MTR_1g082770 | 40.645 | 155 | 90 | 2 | 12 | 164 | 17 | 171 | 4.04e-35 | 123 |
| MsG0580024109.01.T01 | MTR_1g082830 | 33.728 | 169 | 110 | 2 | 15 | 181 | 35 | 203 | 9.43e-31 | 112 |
| MsG0580024109.01.T01 | MTR_1g082790 | 37.391 | 115 | 71 | 1 | 27 | 140 | 46 | 160 | 1.57e-22 | 89.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024109.01.T01 | AT3G06035 | 58.791 | 182 | 72 | 2 | 20 | 201 | 20 | 198 | 5.37e-71 | 214 |
| MsG0580024109.01.T01 | AT5G19250 | 65.972 | 144 | 49 | 0 | 27 | 170 | 28 | 171 | 1.48e-66 | 203 |
| MsG0580024109.01.T01 | AT1G54860 | 41.237 | 194 | 109 | 4 | 5 | 193 | 6 | 199 | 6.41e-51 | 163 |
| MsG0580024109.01.T01 | AT5G19240 | 46.784 | 171 | 77 | 4 | 27 | 194 | 28 | 187 | 7.08e-43 | 143 |
| MsG0580024109.01.T01 | AT5G19230 | 48.485 | 165 | 75 | 3 | 1 | 164 | 1 | 156 | 7.22e-42 | 140 |
| MsG0580024109.01.T01 | AT5G19230 | 48.485 | 165 | 75 | 3 | 1 | 164 | 1 | 156 | 7.22e-42 | 140 |
Find 58 sgRNAs with CRISPR-Local
Find 98 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAAAGCTTTATCACCAAAAT+TGG | 0.148873 | 5:+973528 | MsG0580024109.01.T01:CDS |
| GACTCAAAGTTTACTGGAAT+TGG | 0.202771 | 5:+973402 | MsG0580024109.01.T01:CDS |
| GTAATGCCTGCTTGTGTTCC+TGG | 0.222223 | 5:+973318 | MsG0580024109.01.T01:CDS |
| GGATTGGTTCCGAAGATAAT+TGG | 0.229248 | 5:+973424 | MsG0580024109.01.T01:CDS |
| CTTAATGACTCAAAGTTTAC+TGG | 0.244932 | 5:+973396 | MsG0580024109.01.T01:CDS |
| TTAAGTGACATTTAGTTAAA+AGG | 0.252789 | 5:-973269 | None:intergenic |
| GCAGTAAACCAATCCAATTT+TGG | 0.309912 | 5:-973541 | None:intergenic |
| TGAGGAAGATGATCTTTATC+AGG | 0.311325 | 5:+973077 | MsG0580024109.01.T01:CDS |
| ACAATAAATTGTTCTATTGA+TGG | 0.334563 | 5:+973919 | MsG0580024109.01.T01:three_prime_UTR |
| TTCCTTCTATTCTTCTTCTC+TGG | 0.339502 | 5:-973796 | None:intergenic |
| TTGTGTTCCTGGCCTTGTTC+CGG | 0.361906 | 5:+973329 | MsG0580024109.01.T01:CDS |
| GAAGAAGATACTTGATTTGA+AGG | 0.369222 | 5:-972081 | None:intergenic |
| ACTATCACTGTCTGGGAAAC+GGG | 0.372098 | 5:+973681 | MsG0580024109.01.T01:three_prime_UTR |
| AGTTAAAAGGTCTTCATAGT+TGG | 0.372121 | 5:-973256 | None:intergenic |
| AGTGATAGTATAAGCCTATA+TGG | 0.384109 | 5:-973667 | None:intergenic |
| TGCCAGAGAAGAAGAATAGA+AGG | 0.388717 | 5:+973794 | MsG0580024109.01.T01:three_prime_UTR |
| ACTCAAAGTTTACTGGAATT+GGG | 0.389579 | 5:+973403 | MsG0580024109.01.T01:CDS |
| TACAAGGTTGATTCTTGAAC+TGG | 0.391876 | 5:-973185 | None:intergenic |
| AGGCTTATACTATCACTGTC+TGG | 0.401288 | 5:+973673 | MsG0580024109.01.T01:three_prime_UTR |
| GTTAAAAGGTCTTCATAGTT+GGG | 0.403819 | 5:-973255 | None:intergenic |
| TACTATCACTGTCTGGGAAA+CGG | 0.423338 | 5:+973680 | MsG0580024109.01.T01:three_prime_UTR |
| TGCTCATTGCTCTTGTTAGT+TGG | 0.425769 | 5:+973561 | MsG0580024109.01.T01:CDS |
| AGCATCCTCACGCTCAAAAC+AGG | 0.435655 | 5:-973593 | None:intergenic |
| CTTTATCACCAAAATTGGAT+TGG | 0.444400 | 5:+973533 | MsG0580024109.01.T01:CDS |
| TCAAACTTTGAAGTAACAAA+TGG | 0.445254 | 5:+972117 | MsG0580024109.01.T01:exon |
| AACAACAATCCAATTATCTT+CGG | 0.461927 | 5:-973433 | None:intergenic |
| CTCGTCATCACTAGAACTCT+CGG | 0.472274 | 5:-973493 | None:intergenic |
| AGTGATGACGAGACTTCTAA+TGG | 0.482206 | 5:+973504 | MsG0580024109.01.T01:CDS |
| AAGTTTACTGGAATTGGGAT+TGG | 0.486609 | 5:+973408 | MsG0580024109.01.T01:CDS |
| ACAATGTCCGGAACAAGGCC+AGG | 0.505794 | 5:-973336 | None:intergenic |
| TCTTCATAGTTGGGTAATTG+AGG | 0.505804 | 5:-973246 | None:intergenic |
| TACAGATCCATCCCTTGTGT+TGG | 0.505945 | 5:-973298 | None:intergenic |
| TTGCAGCATAGTCGGTAAGC+AGG | 0.507546 | 5:+973636 | MsG0580024109.01.T01:three_prime_UTR |
| GCACCTGTGGTATTTGTACA+AGG | 0.509795 | 5:-973201 | None:intergenic |
| AATATTTCCAACACAAGGGA+TGG | 0.513314 | 5:+973291 | MsG0580024109.01.T01:CDS |
| ACAAGGCCAGGAACACAAGC+AGG | 0.520245 | 5:-973324 | None:intergenic |
| GGCTTATACTATCACTGTCT+GGG | 0.523682 | 5:+973674 | MsG0580024109.01.T01:three_prime_UTR |
| TATAAGCCTATATGGTAGCT+TGG | 0.531909 | 5:-973659 | None:intergenic |
| GAAGATACTTGATTTGAAGG+TGG | 0.540708 | 5:-972078 | None:intergenic |
| AAATTAGAGAGGACAATGTC+CGG | 0.542734 | 5:-973348 | None:intergenic |
| GGTAATTGAGGCTCAGTACC+TGG | 0.547704 | 5:-973234 | None:intergenic |
| AGCAGGCCAAGCTACCATAT+AGG | 0.557003 | 5:+973653 | MsG0580024109.01.T01:three_prime_UTR |
| TGAGTGACTGAATAAGAGAA+TGG | 0.560522 | 5:-972176 | None:intergenic |
| ACTTAAATATTTCCAACACA+AGG | 0.568858 | 5:+973286 | MsG0580024109.01.T01:CDS |
| TTCAGTGAAGCATAGAGGGA+TGG | 0.576470 | 5:+973732 | MsG0580024109.01.T01:three_prime_UTR |
| TGGTATTGTGTTAGCACCTG+TGG | 0.587893 | 5:-973214 | None:intergenic |
| TCGTCATCACTAGAACTCTC+GGG | 0.593940 | 5:-973492 | None:intergenic |
| GAGGAAGATGATCTTTATCA+GGG | 0.594123 | 5:+973078 | MsG0580024109.01.T01:CDS |
| GAGATTTCGTGAAATTAGAG+AGG | 0.595369 | 5:-973359 | None:intergenic |
| CACTCAGTTCATTGTGACCA+TGG | 0.595566 | 5:+972193 | MsG0580024109.01.T01:CDS |
| CGGGAAACTGCAAATAGTGA+TGG | 0.598265 | 5:+973700 | MsG0580024109.01.T01:three_prime_UTR |
| CAACCTTGTACAAATACCAC+AGG | 0.614385 | 5:+973198 | MsG0580024109.01.T01:CDS |
| AGAGGACAATGTCCGGAACA+AGG | 0.617052 | 5:-973341 | None:intergenic |
| ACAGGTGCTAACACAATACC+AGG | 0.623473 | 5:+973216 | MsG0580024109.01.T01:CDS |
| CTTAAATATTTCCAACACAA+GGG | 0.640170 | 5:+973287 | MsG0580024109.01.T01:CDS |
| GCTTCCGTCAGAAGTGCTTG+TGG | 0.642715 | 5:-973463 | None:intergenic |
| TTGACCACAAGCACTTCTGA+CGG | 0.644420 | 5:+973459 | MsG0580024109.01.T01:CDS |
| ATGTCAAGTTCAATGATGTG+CGG | 0.702075 | 5:-973113 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATAACCAAAATATAATATAT+AGG | + | Chr5:972331-972350 | MsG0580024109.01.T01:intron | 10.0% |
| !!! | AAAGCCTATATATTATATTT+TGG | - | Chr5:972338-972357 | None:intergenic | 15.0% |
| !! | ACAATAAATTGTTCTATTGA+TGG | + | Chr5:973919-973938 | MsG0580024109.01.T01:three_prime_UTR | 20.0% |
| !! | TTAAGTGACATTTAGTTAAA+AGG | - | Chr5:973272-973291 | None:intergenic | 20.0% |
| !!! | AAAAAGCTGTTTTTTTATGT+AGG | + | Chr5:973819-973838 | MsG0580024109.01.T01:three_prime_UTR | 20.0% |
| !!! | AAAAGCTGTTTTTTTATGTA+GGG | + | Chr5:973820-973839 | MsG0580024109.01.T01:three_prime_UTR | 20.0% |
| !!! | AAAGACTTTTACTATAAGTT+GGG | + | Chr5:972519-972538 | MsG0580024109.01.T01:intron | 20.0% |
| !!! | TAAAGACTTTTACTATAAGT+TGG | + | Chr5:972518-972537 | MsG0580024109.01.T01:intron | 20.0% |
| !!! | TAATTTTGCTTCAATATTGT+CGG | + | Chr5:973760-973779 | MsG0580024109.01.T01:three_prime_UTR | 20.0% |
| ! | AACAACAATCCAATTATCTT+CGG | - | Chr5:973436-973455 | None:intergenic | 25.0% |
| ! | ACTAAAATTCAATCACACTA+TGG | - | Chr5:972893-972912 | None:intergenic | 25.0% |
| ! | ACTTAAATATTTCCAACACA+AGG | + | Chr5:973286-973305 | MsG0580024109.01.T01:CDS | 25.0% |
| ! | CTTAAATATTTCCAACACAA+GGG | + | Chr5:973287-973306 | MsG0580024109.01.T01:CDS | 25.0% |
| !! | TTAATTGCTATTATCCACAA+TGG | - | Chr5:972501-972520 | None:intergenic | 25.0% |
| !!! | TCAAACTTTGAAGTAACAAA+TGG | + | Chr5:972117-972136 | MsG0580024109.01.T01:exon | 25.0% |
| ACTCAAAGTTTACTGGAATT+GGG | + | Chr5:973403-973422 | MsG0580024109.01.T01:CDS | 30.0% | |
| AGTGATAGTATAAGCCTATA+TGG | - | Chr5:973670-973689 | None:intergenic | 30.0% | |
| AGTTAAAAGGTCTTCATAGT+TGG | - | Chr5:973259-973278 | None:intergenic | 30.0% | |
| ATAGTTAGATAGATCCATTG+TGG | + | Chr5:972484-972503 | MsG0580024109.01.T01:intron | 30.0% | |
| CTGTATTGAATCAACAAAAC+AGG | - | Chr5:972549-972568 | None:intergenic | 30.0% | |
| CTTAATGACTCAAAGTTTAC+TGG | + | Chr5:973396-973415 | MsG0580024109.01.T01:CDS | 30.0% | |
| CTTTATCACCAAAATTGGAT+TGG | + | Chr5:973533-973552 | MsG0580024109.01.T01:CDS | 30.0% | |
| GTAAGTGTTTATGCTATAAG+CGG | + | Chr5:972840-972859 | MsG0580024109.01.T01:intron | 30.0% | |
| GTTAAAAGGTCTTCATAGTT+GGG | - | Chr5:973258-973277 | None:intergenic | 30.0% | |
| TGAAAGAAGAATAGTTACCA+TGG | - | Chr5:972213-972232 | None:intergenic | 30.0% | |
| ! | AGATACATACAAAGCTTGTT+TGG | + | Chr5:972613-972632 | MsG0580024109.01.T01:intron | 30.0% |
| ! | CATTTTTATTGCAGCATAGT+CGG | + | Chr5:973628-973647 | MsG0580024109.01.T01:three_prime_UTR | 30.0% |
| ! | GAAAGCTTTATCACCAAAAT+TGG | + | Chr5:973528-973547 | MsG0580024109.01.T01:CDS | 30.0% |
| ! | GAAGAAGATACTTGATTTGA+AGG | - | Chr5:972084-972103 | None:intergenic | 30.0% |
| ! | TAAGTTTTCTTTAGTTACCC+AGG | - | Chr5:972760-972779 | None:intergenic | 30.0% |
| AAATTAGAGAGGACAATGTC+CGG | - | Chr5:973351-973370 | None:intergenic | 35.0% | |
| AAGTTTACTGGAATTGGGAT+TGG | + | Chr5:973408-973427 | MsG0580024109.01.T01:CDS | 35.0% | |
| AATATTTCCAACACAAGGGA+TGG | + | Chr5:973291-973310 | MsG0580024109.01.T01:CDS | 35.0% | |
| ACACACTTACGAGATTGTTT+AGG | + | Chr5:972666-972685 | MsG0580024109.01.T01:intron | 35.0% | |
| AGAACTAGCTAGATCTGATA+GGG | + | Chr5:972277-972296 | MsG0580024109.01.T01:intron | 35.0% | |
| ATGTCAAGTTCAATGATGTG+CGG | - | Chr5:973116-973135 | None:intergenic | 35.0% | |
| GACTCAAAGTTTACTGGAAT+TGG | + | Chr5:973402-973421 | MsG0580024109.01.T01:CDS | 35.0% | |
| GAGATTTCGTGAAATTAGAG+AGG | - | Chr5:973362-973381 | None:intergenic | 35.0% | |
| GAGGAAGATGATCTTTATCA+GGG | + | Chr5:973078-973097 | MsG0580024109.01.T01:CDS | 35.0% | |
| TAGAACTAGCTAGATCTGAT+AGG | + | Chr5:972276-972295 | MsG0580024109.01.T01:intron | 35.0% | |
| TATAAGCCTATATGGTAGCT+TGG | - | Chr5:973662-973681 | None:intergenic | 35.0% | |
| TCTTCATAGTTGGGTAATTG+AGG | - | Chr5:973249-973268 | None:intergenic | 35.0% | |
| TGAGGAAGATGATCTTTATC+AGG | + | Chr5:973077-973096 | MsG0580024109.01.T01:CDS | 35.0% | |
| TGAGTGACTGAATAAGAGAA+TGG | - | Chr5:972179-972198 | None:intergenic | 35.0% | |
| TTATTCCCATAAGCTTTCCT+GGG | + | Chr5:972740-972759 | MsG0580024109.01.T01:intron | 35.0% | |
| TTCCTTCTATTCTTCTTCTC+TGG | - | Chr5:973799-973818 | None:intergenic | 35.0% | |
| TTGAGTGAATCTATCTCTCT+TGG | + | Chr5:972935-972954 | MsG0580024109.01.T01:intron | 35.0% | |
| ! | GAAGATACTTGATTTGAAGG+TGG | - | Chr5:972081-972100 | None:intergenic | 35.0% |
| ! | GCAGTAAACCAATCCAATTT+TGG | - | Chr5:973544-973563 | None:intergenic | 35.0% |
| ! | TACAAGGTTGATTCTTGAAC+TGG | - | Chr5:973188-973207 | None:intergenic | 35.0% |
| !! | AGATCTAGCTAGTTCTACAT+TGG | - | Chr5:972273-972292 | None:intergenic | 35.0% |
| !! | GTTTTTCAGTGAAGCATAGA+GGG | + | Chr5:973728-973747 | MsG0580024109.01.T01:three_prime_UTR | 35.0% |
| !! | TGTTTTTCAGTGAAGCATAG+AGG | + | Chr5:973727-973746 | MsG0580024109.01.T01:three_prime_UTR | 35.0% |
| AGGCTTATACTATCACTGTC+TGG | + | Chr5:973673-973692 | MsG0580024109.01.T01:three_prime_UTR | 40.0% | |
| AGTGAATCTATCTCTCTTGG+TGG | + | Chr5:972938-972957 | MsG0580024109.01.T01:intron | 40.0% | |
| AGTTACCCAGGAAAGCTTAT+GGG | - | Chr5:972748-972767 | None:intergenic | 40.0% | |
| CAACCTTGTACAAATACCAC+AGG | + | Chr5:973198-973217 | MsG0580024109.01.T01:CDS | 40.0% | |
| CTTATTCCCATAAGCTTTCC+TGG | + | Chr5:972739-972758 | MsG0580024109.01.T01:intron | 40.0% | |
| GAACTAGCTAGATCTGATAG+GGG | + | Chr5:972278-972297 | MsG0580024109.01.T01:intron | 40.0% | |
| GGCTTATACTATCACTGTCT+GGG | + | Chr5:973674-973693 | MsG0580024109.01.T01:three_prime_UTR | 40.0% | |
| TACTATCACTGTCTGGGAAA+CGG | + | Chr5:973680-973699 | MsG0580024109.01.T01:three_prime_UTR | 40.0% | |
| TAGTTACCCAGGAAAGCTTA+TGG | - | Chr5:972749-972768 | None:intergenic | 40.0% | |
| TGCCAGAGAAGAAGAATAGA+AGG | + | Chr5:973794-973813 | MsG0580024109.01.T01:three_prime_UTR | 40.0% | |
| TGCTCATTGCTCTTGTTAGT+TGG | + | Chr5:973561-973580 | MsG0580024109.01.T01:CDS | 40.0% | |
| ! | AGTGATGACGAGACTTCTAA+TGG | + | Chr5:973504-973523 | MsG0580024109.01.T01:CDS | 40.0% |
| !! | ATTATCCTGTTTTGAGCGTG+AGG | + | Chr5:973588-973607 | MsG0580024109.01.T01:exon | 40.0% |
| !! | GGATTGGTTCCGAAGATAAT+TGG | + | Chr5:973424-973443 | MsG0580024109.01.T01:CDS | 40.0% |
| ACAGGTGCTAACACAATACC+AGG | + | Chr5:973216-973235 | MsG0580024109.01.T01:CDS | 45.0% | |
| ACTATCACTGTCTGGGAAAC+GGG | + | Chr5:973681-973700 | MsG0580024109.01.T01:three_prime_UTR | 45.0% | |
| AGATCATCTTCCTCATCTGC+AGG | - | Chr5:973072-973091 | None:intergenic | 45.0% | |
| AGCGATAAGATCAGCGAATC+TGG | + | Chr5:972567-972586 | MsG0580024109.01.T01:intron | 45.0% | |
| ATCATCTTCCTCATCTGCAG+GGG | - | Chr5:973070-973089 | None:intergenic | 45.0% | |
| CACTCAGTTCATTGTGACCA+TGG | + | Chr5:972193-972212 | MsG0580024109.01.T01:CDS | 45.0% | |
| CGGGAAACTGCAAATAGTGA+TGG | + | Chr5:973700-973719 | MsG0580024109.01.T01:three_prime_UTR | 45.0% | |
| CTCGTCATCACTAGAACTCT+CGG | - | Chr5:973496-973515 | None:intergenic | 45.0% | |
| GATCATCTTCCTCATCTGCA+GGG | - | Chr5:973071-973090 | None:intergenic | 45.0% | |
| GCACCTGTGGTATTTGTACA+AGG | - | Chr5:973204-973223 | None:intergenic | 45.0% | |
| TACAGATCCATCCCTTGTGT+TGG | - | Chr5:973301-973320 | None:intergenic | 45.0% | |
| TCGTCATCACTAGAACTCTC+GGG | - | Chr5:973495-973514 | None:intergenic | 45.0% | |
| TGGTATTGTGTTAGCACCTG+TGG | - | Chr5:973217-973236 | None:intergenic | 45.0% | |
| TTCAGTGAAGCATAGAGGGA+TGG | + | Chr5:973732-973751 | MsG0580024109.01.T01:three_prime_UTR | 45.0% | |
| TTGACCACAAGCACTTCTGA+CGG | + | Chr5:973459-973478 | MsG0580024109.01.T01:CDS | 45.0% | |
| ! | ATCTATCTCTCTTGGTGGAC+TGG | + | Chr5:972943-972962 | MsG0580024109.01.T01:intron | 45.0% |
| AGAGGACAATGTCCGGAACA+AGG | - | Chr5:973344-973363 | None:intergenic | 50.0% | |
| AGCAGGCCAAGCTACCATAT+AGG | + | Chr5:973653-973672 | MsG0580024109.01.T01:three_prime_UTR | 50.0% | |
| AGCATCCTCACGCTCAAAAC+AGG | - | Chr5:973596-973615 | None:intergenic | 50.0% | |
| GGACTGGATCAAGCACAAGT+TGG | + | Chr5:972959-972978 | MsG0580024109.01.T01:intron | 50.0% | |
| GGTAATTGAGGCTCAGTACC+TGG | - | Chr5:973237-973256 | None:intergenic | 50.0% | |
| GTAATGCCTGCTTGTGTTCC+TGG | + | Chr5:973318-973337 | MsG0580024109.01.T01:CDS | 50.0% | |
| TCATCTTCCTCATCTGCAGG+GGG | - | Chr5:973069-973088 | None:intergenic | 50.0% | |
| TTGCAGCATAGTCGGTAAGC+AGG | + | Chr5:973636-973655 | MsG0580024109.01.T01:three_prime_UTR | 50.0% | |
| !! | TTGTGTTCCTGGCCTTGTTC+CGG | + | Chr5:973329-973348 | MsG0580024109.01.T01:CDS | 50.0% |
| ACAAGGCCAGGAACACAAGC+AGG | - | Chr5:973327-973346 | None:intergenic | 55.0% | |
| ACAATGTCCGGAACAAGGCC+AGG | - | Chr5:973339-973358 | None:intergenic | 55.0% | |
| CATCTGCAGGGGGGATGTTA+AGG | - | Chr5:973059-973078 | None:intergenic | 55.0% | |
| CATCTTCCTCATCTGCAGGG+GGG | - | Chr5:973068-973087 | None:intergenic | 55.0% | |
| TAACATCCCCCCTGCAGATG+AGG | + | Chr5:973059-973078 | MsG0580024109.01.T01:intron | 55.0% | |
| !! | GCTTCCGTCAGAAGTGCTTG+TGG | - | Chr5:973466-973485 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 972061 | 973962 | 972061 | ID=MsG0580024109.01;Name=MsG0580024109.01 |
| Chr5 | mRNA | 972061 | 973962 | 972061 | ID=MsG0580024109.01.T01;Parent=MsG0580024109.01;Name=MsG0580024109.01.T01;_AED=0.28;_eAED=0.28;_QI=75|1|1|1|0|0.5|2|360|201 |
| Chr5 | exon | 972061 | 972214 | 972061 | ID=MsG0580024109.01.T01:exon:293;Parent=MsG0580024109.01.T01 |
| Chr5 | exon | 973076 | 973962 | 973076 | ID=MsG0580024109.01.T01:exon:294;Parent=MsG0580024109.01.T01 |
| Chr5 | five_prime_UTR | 972061 | 972135 | 972061 | ID=MsG0580024109.01.T01:five_prime_utr;Parent=MsG0580024109.01.T01 |
| Chr5 | CDS | 972136 | 972214 | 972136 | ID=MsG0580024109.01.T01:cds;Parent=MsG0580024109.01.T01 |
| Chr5 | CDS | 973076 | 973602 | 973076 | ID=MsG0580024109.01.T01:cds;Parent=MsG0580024109.01.T01 |
| Chr5 | three_prime_UTR | 973603 | 973962 | 973603 | ID=MsG0580024109.01.T01:three_prime_utr;Parent=MsG0580024109.01.T01 |
| Gene Sequence |
| Protein sequence |
