AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480020944.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480020944.01.T01 MTR_4g063780 95.833 336 11 2 1 335 1 334 0.0 587
MsG0480020944.01.T01 MTR_2g096740 42.061 359 128 17 1 335 1 303 3.41e-65 208
MsG0480020944.01.T01 MTR_7g059400 43.769 329 135 10 1 311 1 297 1.77e-63 205
MsG0480020944.01.T01 MTR_2g096740 39.554 359 122 15 1 335 1 288 3.96e-59 192
MsG0480020944.01.T01 MTR_4g022370 40.346 347 152 13 1 316 1 323 3.03e-57 189
MsG0480020944.01.T01 MTR_7g024670 59.028 144 52 4 1 139 1 142 1.77e-46 162
MsG0480020944.01.T01 MTR_5g031440 87.500 56 7 0 70 125 38 93 1.85e-32 124
MsG0480020944.01.T01 MTR_3g077750 54.867 113 44 3 48 160 37 142 2.34e-32 123
MsG0480020944.01.T01 MTR_8g479350 60.674 89 26 1 75 163 68 147 1.05e-31 122
MsG0480020944.01.T01 MTR_2g093220 80.328 61 12 0 69 129 34 94 2.29e-31 119
MsG0480020944.01.T01 MTR_4g461080 66.667 78 25 1 50 127 5 81 2.72e-31 119
MsG0480020944.01.T01 MTR_4g088580 42.604 169 69 5 76 221 56 219 1.17e-30 120
MsG0480020944.01.T01 MTR_8g068210 75.758 66 13 1 74 139 31 93 2.10e-30 118
MsG0480020944.01.T01 MTR_8g027295 72.059 68 19 0 62 129 11 78 3.72e-30 116
MsG0480020944.01.T01 MTR_3g091820 67.089 79 18 2 56 134 28 98 1.13e-29 115
MsG0480020944.01.T01 MTR_1g077600 60.000 80 30 1 52 129 18 97 3.95e-29 113
MsG0480020944.01.T01 MTR_8g079060 53.774 106 33 3 54 159 17 106 4.25e-29 112
MsG0480020944.01.T01 MTR_1g077600 60.000 80 30 1 52 129 18 97 4.26e-29 114
MsG0480020944.01.T01 MTR_3g090430 63.514 74 27 0 56 129 32 105 1.26e-28 113
MsG0480020944.01.T01 MTR_8g015840 58.750 80 30 1 59 135 1 80 1.64e-28 110
MsG0480020944.01.T01 MTR_2g014060 67.606 71 22 1 57 126 6 76 2.84e-28 112
MsG0480020944.01.T01 MTR_4g109980 82.692 52 9 0 74 125 39 90 4.26e-28 111
MsG0480020944.01.T01 MTR_2g014170 88.000 50 6 0 74 123 44 93 6.85e-28 110
MsG0480020944.01.T01 MTR_1g056810 75.862 58 14 0 77 134 14 71 9.70e-28 110
MsG0480020944.01.T01 MTR_2g059540 84.000 50 8 0 74 123 30 79 9.97e-27 105
MsG0480020944.01.T01 MTR_4g089095 71.186 59 15 1 75 133 50 106 5.99e-25 102
MsG0480020944.01.T01 MTR_4g089095 71.186 59 15 1 75 133 16 72 7.63e-25 101
MsG0480020944.01.T01 MTR_2g013370 66.129 62 16 1 74 135 45 101 2.46e-24 100
MsG0480020944.01.T01 MTR_8g044220 66.102 59 20 0 71 129 114 172 3.12e-24 102
MsG0480020944.01.T01 MTR_2g016030 64.286 70 24 1 72 141 44 112 4.33e-24 97.1
MsG0480020944.01.T01 MTR_6g027450 63.333 60 22 0 68 127 82 141 8.17e-24 100
MsG0480020944.01.T01 MTR_6g012450 66.667 60 20 0 68 127 129 188 1.43e-23 101
MsG0480020944.01.T01 MTR_4g082060 58.571 70 26 1 58 127 108 174 1.97e-23 100
MsG0480020944.01.T01 MTR_7g086780 60.000 70 25 2 60 129 86 152 2.75e-23 100
MsG0480020944.01.T01 MTR_7g010950 63.333 60 22 0 68 127 128 187 2.99e-23 100
MsG0480020944.01.T01 MTR_3g435480 62.687 67 24 1 68 134 129 194 3.82e-23 100
MsG0480020944.01.T01 MTR_6g027460 61.667 60 23 0 68 127 89 148 5.41e-23 98.6
MsG0480020944.01.T01 MTR_5g041420 43.820 89 50 0 39 127 102 190 1.35e-21 94.0
MsG0480020944.01.T01 MTR_5g041530 69.091 55 16 1 77 131 142 195 1.79e-20 91.7
MsG0480020944.01.T01 MTR_5g041380 48.780 82 41 1 59 140 111 191 7.05e-20 89.7
MsG0480020944.01.T01 MTR_5g041400 50.725 69 34 0 59 127 103 171 1.53e-19 88.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480020944.01.T01 AT3G55370 38.920 352 137 14 1 323 1 303 9.45e-52 174
MsG0480020944.01.T01 AT3G55370 38.211 369 147 16 1 323 1 334 1.31e-50 172
MsG0480020944.01.T01 AT1G07640 41.667 288 143 8 57 334 3 275 2.40e-50 169
MsG0480020944.01.T01 AT1G07640 41.667 288 143 8 57 334 59 331 7.62e-50 169
MsG0480020944.01.T01 AT1G07640 41.667 288 143 8 57 334 67 339 1.38e-49 169
MsG0480020944.01.T01 AT3G55370 39.936 313 118 11 32 323 85 348 4.74e-49 168
MsG0480020944.01.T01 AT2G37590 53.846 156 32 2 1 129 1 143 1.27e-46 161
MsG0480020944.01.T01 AT2G28810 38.244 353 174 15 1 331 1 331 1.47e-45 158
MsG0480020944.01.T01 AT5G02460 88.158 76 9 0 54 129 74 149 6.44e-44 155
MsG0480020944.01.T01 AT2G28810 40.860 279 139 10 57 331 58 314 3.34e-42 149
MsG0480020944.01.T01 AT5G60200 76.389 72 13 1 70 141 50 117 2.61e-34 126
MsG0480020944.01.T01 AT1G51700 58.889 90 29 1 70 159 28 109 1.35e-31 117
MsG0480020944.01.T01 AT5G60850 66.667 78 22 1 74 151 50 123 8.44e-31 119
MsG0480020944.01.T01 AT3G45610 65.672 67 23 0 70 136 35 101 2.10e-30 116
MsG0480020944.01.T01 AT4G38000 77.049 61 14 0 69 129 35 95 2.41e-30 115
MsG0480020944.01.T01 AT5G65590 81.034 58 11 0 75 132 41 98 5.74e-30 116
MsG0480020944.01.T01 AT3G21270 82.143 56 10 0 75 130 29 84 4.59e-29 111
MsG0480020944.01.T01 AT4G24060 50.000 112 56 0 69 180 47 158 1.37e-28 113
MsG0480020944.01.T01 AT3G52440 70.588 68 16 1 77 144 27 90 4.51e-28 110
MsG0480020944.01.T01 AT1G28310 79.661 59 11 1 75 133 27 84 4.60e-28 111
MsG0480020944.01.T01 AT3G52440 70.588 68 16 1 77 144 46 109 5.73e-28 110
MsG0480020944.01.T01 AT1G28310 79.661 59 11 1 75 133 51 108 6.60e-28 111
MsG0480020944.01.T01 AT2G28510 73.846 65 13 1 74 138 47 107 6.67e-28 110
MsG0480020944.01.T01 AT5G62940 77.193 57 13 0 69 125 67 123 8.67e-28 112
MsG0480020944.01.T01 AT5G66940 65.714 70 21 1 75 144 32 98 2.98e-27 107
MsG0480020944.01.T01 AT3G61850 73.438 64 13 1 71 134 58 117 3.25e-27 108
MsG0480020944.01.T01 AT3G61850 73.438 64 13 1 71 134 58 117 3.25e-27 108
MsG0480020944.01.T01 AT3G61850 40.132 152 62 3 71 222 58 180 3.47e-27 108
MsG0480020944.01.T01 AT3G61850 73.438 64 13 1 71 134 70 129 3.55e-27 108
MsG0480020944.01.T01 AT1G21340 71.875 64 12 1 74 137 37 94 4.96e-27 107
MsG0480020944.01.T01 AT3G61850 40.132 152 62 3 71 222 70 192 5.62e-27 108
MsG0480020944.01.T01 AT4G21050 71.186 59 17 0 68 126 17 75 1.13e-26 105
MsG0480020944.01.T01 AT3G50410 70.690 58 17 0 75 132 30 87 1.61e-26 105
MsG0480020944.01.T01 AT2G46590 49.091 110 49 2 13 122 13 115 2.84e-26 107
MsG0480020944.01.T01 AT2G46590 49.091 110 49 2 13 122 25 127 3.53e-26 107
MsG0480020944.01.T01 AT4G00940 63.514 74 23 2 59 129 50 122 1.58e-25 104
MsG0480020944.01.T01 AT4G00940 63.514 74 23 2 59 129 50 122 1.58e-25 104
MsG0480020944.01.T01 AT4G00940 63.514 74 23 2 59 129 50 122 1.58e-25 104
MsG0480020944.01.T01 AT1G64620 65.217 69 24 0 56 124 30 98 5.45e-25 103
MsG0480020944.01.T01 AT1G47655 70.000 60 16 1 66 123 18 77 2.38e-24 99.0
MsG0480020944.01.T01 AT2G34140 58.442 77 28 2 53 129 39 111 4.46e-24 97.1
MsG0480020944.01.T01 AT1G29160 69.492 59 17 1 71 129 58 115 1.36e-23 95.9
MsG0480020944.01.T01 AT5G39660 65.574 61 21 0 67 127 130 190 4.23e-23 99.8
MsG0480020944.01.T01 AT5G39660 65.574 61 21 0 67 127 130 190 4.23e-23 99.8
MsG0480020944.01.T01 AT5G39660 65.574 61 21 0 67 127 130 190 4.23e-23 99.8
MsG0480020944.01.T01 AT1G26790 54.412 68 31 0 60 127 96 163 4.68e-23 99.0
MsG0480020944.01.T01 AT5G62430 56.000 75 31 2 58 131 36 109 5.00e-23 97.4
MsG0480020944.01.T01 AT4G21040 66.667 63 16 1 77 139 27 84 6.03e-23 95.9
MsG0480020944.01.T01 AT3G47500 58.824 68 28 0 68 135 103 170 9.96e-23 98.6
MsG0480020944.01.T01 AT1G69570 61.667 60 23 0 68 127 125 184 2.41e-22 97.1
MsG0480020944.01.T01 AT4G21080 63.492 63 18 1 77 139 27 84 9.20e-22 92.8
MsG0480020944.01.T01 AT4G21030 54.098 61 25 1 68 128 17 74 2.24e-15 73.9

Find 108 sgRNAs with CRISPR-Local

Find 137 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCAAGACATGTAGGCGTTAT+TGG 0.207030 4:+50958430 MsG0480020944.01.T01:CDS
GTTGTTGTTGATGTTGTTCT+TGG 0.217968 4:-50958722 None:intergenic
GATGAGAGAATGAGGGAATA+AGG 0.227115 4:+50957751 MsG0480020944.01.T01:five_prime_UTR
CCATTTGCTTGCTGATGATT+TGG 0.240767 4:-50958183 None:intergenic
TCAAGAGCCACCGGTTAAAA+TGG 0.246428 4:+50958992 MsG0480020944.01.T01:CDS
TCTTGAGAGTTTGTTGAATT+AGG 0.247926 4:-50958540 None:intergenic
ACCAACAACAATACTATTCT+TGG 0.250684 4:+50959072 MsG0480020944.01.T01:CDS
GTTTCTGCTTCTGCTGTTGC+TGG 0.257390 4:+50958804 MsG0480020944.01.T01:CDS
TACTGGTGGTGGTGGTACTA+TGG 0.263264 4:+50958257 MsG0480020944.01.T01:CDS
GATCATATGGGGTTTCAAAT+TGG 0.271163 4:+50958750 MsG0480020944.01.T01:CDS
TTTATAAAAGAAAACATAAA+AGG 0.276480 4:+50957910 MsG0480020944.01.T01:five_prime_UTR
TATTCTTGGAATGATTTATC+AGG 0.288988 4:+50959086 MsG0480020944.01.T01:CDS
TTTACCTGGTGGTGCCAATT+AGG 0.304320 4:-50957981 None:intergenic
CCTTCTCATCATGAACTAAT+TGG 0.327970 4:+50958591 MsG0480020944.01.T01:CDS
TTCATATCTTTGATCCATTC+CGG 0.333436 4:-50959140 None:intergenic
GTTATGTAGATCCTCCTAAT+TGG 0.350822 4:+50957967 MsG0480020944.01.T01:CDS
TTCTCTTCTCTGTATTCCCT+TGG 0.355868 4:+50957849 MsG0480020944.01.T01:five_prime_UTR
AATTCCTAACCTACCCTTCA+TGG 0.365060 4:+50958632 MsG0480020944.01.T01:CDS
ACAACAACAACAAGATCATA+TGG 0.370266 4:+50958737 MsG0480020944.01.T01:CDS
AATATTGAAGCTGCAGCATC+TGG 0.370611 4:+50958933 MsG0480020944.01.T01:CDS
ATGAGAGAATGAGGGAATAA+GGG 0.394979 4:+50957752 MsG0480020944.01.T01:five_prime_UTR
TCTGCTGTTGCTGGAGGTGT+TGG 0.398368 4:+50958813 MsG0480020944.01.T01:CDS
AATCTGAGTCGTTACGTTGT+TGG 0.398820 4:+50958666 MsG0480020944.01.T01:CDS
TTTCAAATTGGTATTGGTAA+TGG 0.399190 4:+50958762 MsG0480020944.01.T01:CDS
TCCAAGAATAGTATTGTTGT+TGG 0.406762 4:-50959073 None:intergenic
ATGGGGTTTCAAATTGGTAT+TGG 0.408013 4:+50958756 MsG0480020944.01.T01:CDS
AATACAGAGAAGAGAAAGAA+AGG 0.409902 4:-50957842 None:intergenic
CTCCCACTTGAGGTGGTAAA+GGG 0.410485 4:-50958230 None:intergenic
CTACTCATCTCTTATAACTT+TGG 0.422294 4:+50959117 MsG0480020944.01.T01:exon
GGACACTTTAGTGCTGGTTC+TGG 0.427397 4:-50958336 None:intergenic
CCTCCCACTTGAGGTGGTAA+AGG 0.434525 4:-50958231 None:intergenic
CTGACGTTGTAATATCAATT+CGG 0.439770 4:+50959270 MsG0480020944.01.T01:three_prime_UTR
ATATTACTTTCTCCTTGAAA+AGG 0.442140 4:-50958915 None:intergenic
GCTCGATCAGCCATGGAGCC+TGG 0.444337 4:-50958291 None:intergenic
GTGTAAAGTAAGTAAACTTA+AGG 0.447478 4:-50959304 None:intergenic
CCTCAAGTGGGAGGTAGTAC+TGG 0.448394 4:+50958240 MsG0480020944.01.T01:CDS
CCAAATCATCAGCAAGCAAA+TGG 0.450775 4:+50958183 MsG0480020944.01.T01:CDS
GGAAACGTTAACATGGGTCT+TGG 0.451197 4:+50958687 MsG0480020944.01.T01:CDS
TTGATGGCTCTTTAGGGAGA+TGG 0.453212 4:-50957884 None:intergenic
GCTGCAGCATCTGGATTTGT+TGG 0.454209 4:+50958942 MsG0480020944.01.T01:CDS
ACTGGTGGTGGTGGTACTAT+GGG 0.454422 4:+50958258 MsG0480020944.01.T01:CDS
GATATGAAGTTTCGAACGAT+TGG 0.454492 4:+50959155 MsG0480020944.01.T01:three_prime_UTR
TCTTATAACTTTGGCCGGAA+TGG 0.459551 4:+50959126 MsG0480020944.01.T01:exon
TTTAGTGCTGGTTCTGGTGG+TGG 0.460208 4:-50958330 None:intergenic
GATTCAAAAGTATTCAAGAA+AGG 0.460724 4:-50958867 None:intergenic
CTACGACACCCTCCACCAAC+AGG 0.463438 4:-50958480 None:intergenic
AATTTATCAAGAACATCTCT+TGG 0.474556 4:+50959032 MsG0480020944.01.T01:CDS
CTCGATCAGACCAGGCTCCA+TGG 0.475078 4:+50958281 MsG0480020944.01.T01:CDS
GAGATATAGCTTCAAACTCT+AGG 0.480948 4:+50958964 MsG0480020944.01.T01:CDS
ATACAGAGAAGAGAAAGAAA+GGG 0.484195 4:-50957841 None:intergenic
GGTAGGTTAGGAATTGTTTG+TGG 0.484498 4:-50958624 None:intergenic
CAACAACAACAAGATCATAT+GGG 0.484986 4:+50958738 MsG0480020944.01.T01:CDS
CGTTGTTGGAAACGTTAACA+TGG 0.486766 4:+50958680 MsG0480020944.01.T01:CDS
TAAGGAAGAGAGGAGGGAGG+AGG 0.490035 4:-50957810 None:intergenic
GAGGCCATGAAGGGTAGGTT+AGG 0.490552 4:-50958636 None:intergenic
CTTAGAAATGTTCCTGTTGG+TGG 0.493876 4:+50958468 MsG0480020944.01.T01:CDS
CACTTTAGTGCTGGTTCTGG+TGG 0.506223 4:-50958333 None:intergenic
TCATCTCTTATAACTTTGGC+CGG 0.512022 4:+50959121 MsG0480020944.01.T01:exon
GGAGGTAGTACTGGTGGTGG+TGG 0.514269 4:+50958249 MsG0480020944.01.T01:CDS
CCAATTAGTTCATGATGAGA+AGG 0.516486 4:-50958591 None:intergenic
CAACGAGGACACTTTAGTGC+TGG 0.521891 4:-50958342 None:intergenic
AGAAATGTTCCTGTTGGTGG+AGG 0.523012 4:+50958471 MsG0480020944.01.T01:CDS
GAAGTAGCAAAATTTAGTGT+TGG 0.523027 4:-50958373 None:intergenic
AAAGATTAAGGAAGAGAGGA+GGG 0.523529 4:-50957816 None:intergenic
TCCTCCTAATTGGCACCACC+AGG 0.528426 4:+50957977 MsG0480020944.01.T01:CDS
ACCTGGTGGTGCCAATTAGG+AGG 0.541280 4:-50957978 None:intergenic
TCTGCTTCTGCTGTTGCTGG+AGG 0.548891 4:+50958807 MsG0480020944.01.T01:CDS
AAAAGATTAAGGAAGAGAGG+AGG 0.549849 4:-50957817 None:intergenic
ATGGCTCTTTAGGGAGATGG+TGG 0.554378 4:-50957881 None:intergenic
AGATATAGCTTCAAACTCTA+GGG 0.556521 4:+50958965 MsG0480020944.01.T01:CDS
ATTCTGCATCGAGGCCATGA+AGG 0.561082 4:-50958646 None:intergenic
CAATTAGTTCATGATGAGAA+GGG 0.573984 4:-50958590 None:intergenic
GCACTTAGAAATGTTCCTGT+TGG 0.577935 4:+50958465 MsG0480020944.01.T01:CDS
ACTATGGGCTCGATCAGACC+AGG 0.580839 4:+50958273 MsG0480020944.01.T01:CDS
TTCTGCATCGAGGCCATGAA+GGG 0.581643 4:-50958645 None:intergenic
AGAAGGGATTGCACATGATG+AGG 0.582276 4:-50958574 None:intergenic
GTGGGAGGTAGTACTGGTGG+TGG 0.591143 4:+50958246 MsG0480020944.01.T01:CDS
GAAATGTTCCTGTTGGTGGA+GGG 0.600130 4:+50958472 MsG0480020944.01.T01:CDS
TATATTATTCAACTCAGTCA+TGG 0.602165 4:+50959203 MsG0480020944.01.T01:three_prime_UTR
GTAGGCGTTATTGGACAAGA+GGG 0.605161 4:+50958439 MsG0480020944.01.T01:CDS
GATTAAGGAAGAGAGGAGGG+AGG 0.605195 4:-50957813 None:intergenic
TAGGGCTAATCAAGAGCCAC+CGG 0.605644 4:+50958983 MsG0480020944.01.T01:CDS
GTCACTTCTGCAAGACATGT+AGG 0.611130 4:+50958421 MsG0480020944.01.T01:CDS
TGTAGGCGTTATTGGACAAG+AGG 0.616737 4:+50958438 MsG0480020944.01.T01:CDS
GCATCGAGGCCATGAAGGGT+AGG 0.623817 4:-50958641 None:intergenic
GTTGTTGGAAACGTTAACAT+GGG 0.627147 4:+50958681 MsG0480020944.01.T01:CDS
AATTGCTGCAAATTACGCCA+TGG 0.629104 4:-50958843 None:intergenic
TAAACGAGCTCGATCAGCCA+TGG 0.633447 4:-50958298 None:intergenic
AGGAGGAAAGCAGCAATGAG+AGG 0.635016 4:-50957793 None:intergenic
CATGTCTTGCAGAAGTGACG+AGG 0.635723 4:-50958417 None:intergenic
TCCCACTTGAGGTGGTAAAG+GGG 0.637701 4:-50958229 None:intergenic
CCCACTTGAGGTGGTAAAGG+GGG 0.637883 4:-50958228 None:intergenic
TTCTTGATAAATTTAAAGCG+CGG 0.644934 4:-50959022 None:intergenic
AACAACAACAAGATCATATG+GGG 0.646211 4:+50958739 MsG0480020944.01.T01:CDS
CAAGTGGGAGGTAGTACTGG+TGG 0.646524 4:+50958243 MsG0480020944.01.T01:CDS
GTACTACCTCCCACTTGAGG+TGG 0.666584 4:-50958237 None:intergenic
TAGGCGTTATTGGACAAGAG+GGG 0.675252 4:+50958440 MsG0480020944.01.T01:CDS
AGGCGTTATTGGACAAGAGG+GGG 0.675862 4:+50958441 MsG0480020944.01.T01:CDS
CCTTTACCACCTCAAGTGGG+AGG 0.680714 4:+50958231 MsG0480020944.01.T01:CDS
ATGGTGGAGAATCAAACCAA+GGG 0.681127 4:-50957865 None:intergenic
GATGGTGGAGAATCAAACCA+AGG 0.687483 4:-50957866 None:intergenic
CCAGTACTACCTCCCACTTG+AGG 0.688806 4:-50958240 None:intergenic
GAGGTGTTGGAGTTGAACCA+TGG 0.701694 4:+50958826 MsG0480020944.01.T01:CDS
TGAGTTGAATAATATATGAG+TGG 0.703462 4:-50959196 None:intergenic
TTGAATAGAAGAGAAAACCA+TGG 0.707467 4:-50957943 None:intergenic
AGAAGTGACGAGGTTGAGTG+AGG 0.724186 4:-50958407 None:intergenic
GTGTTGGTTGAATCGCAACG+AGG 0.733913 4:-50958357 None:intergenic
ACGACTCAGATTCTGCATCG+AGG 0.740811 4:-50958655 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTATAAAAGAAAACATAAA+AGG + Chr4:50957910-50957929 MsG0480020944.01.T01:five_prime_UTR 10.0%
!!! ATTTTAAAAAATTATTGTGA+TGG - Chr4:50958120-50958139 None:intergenic 10.0%
!! ATGGAAAGAAATTAAAAAAA+GGG - Chr4:50958144-50958163 None:intergenic 15.0%
!!! TTTCTTTTATAAAGTTTTGA+TGG - Chr4:50957903-50957922 None:intergenic 15.0%
!! ATGAAAATTAAAACTTTACC+TGG - Chr4:50957998-50958017 None:intergenic 20.0%
!! GATGGAAAGAAATTAAAAAA+AGG - Chr4:50958145-50958164 None:intergenic 20.0%
!!! AATAATTCATATCCTTTTCA+AGG + Chr4:50958903-50958922 MsG0480020944.01.T01:CDS 20.0%
!!! TATATTTTTAGGGTTTTTAG+TGG - Chr4:50957717-50957736 None:intergenic 20.0%
!!! TTCACTTTCTTTTTTGAATT+TGG - Chr4:50958027-50958046 None:intergenic 20.0%
! AAAATTAAAACTTTACCTGG+TGG - Chr4:50957995-50958014 None:intergenic 25.0%
! AAAGAAAGGGAAAAAGATTA+AGG - Chr4:50957831-50957850 None:intergenic 25.0%
! AATTTATCAAGAACATCTCT+TGG + Chr4:50959032-50959051 MsG0480020944.01.T01:CDS 25.0%
! ATATTACTTTCTCCTTGAAA+AGG - Chr4:50958918-50958937 None:intergenic 25.0%
! ATATTCAAGAAGATGAAGTA+AGG - Chr4:50958084-50958103 None:intergenic 25.0%
! CCTTAAGTTTACTTACTTTA+AGG + Chr4:50959304-50959323 MsG0480020944.01.T01:three_prime_UTR 25.0%
! TATATTATTCAACTCAGTCA+TGG + Chr4:50959203-50959222 MsG0480020944.01.T01:three_prime_UTR 25.0%
! TATTCTTGGAATGATTTATC+AGG + Chr4:50959086-50959105 MsG0480020944.01.T01:CDS 25.0%
!! TAAAAGGATTTTCAATACCA+TGG + Chr4:50957926-50957945 MsG0480020944.01.T01:exon 25.0%
!! TGAGTTGAATAATATATGAG+TGG - Chr4:50959199-50959218 None:intergenic 25.0%
!! TTCTTGATAAATTTAAAGCG+CGG - Chr4:50959025-50959044 None:intergenic 25.0%
!! TTGAATTAGGAGATTTTGAA+CGG - Chr4:50958530-50958549 None:intergenic 25.0%
!!! GATTCAAAAGTATTCAAGAA+AGG - Chr4:50958870-50958889 None:intergenic 25.0%
!!! TTTCAAATTGGTATTGGTAA+TGG + Chr4:50958762-50958781 MsG0480020944.01.T01:CDS 25.0%
AAAAAGAGATGAGAGAATGA+GGG + Chr4:50957744-50957763 MsG0480020944.01.T01:five_prime_UTR 30.0%
AACAACAACAAGATCATATG+GGG + Chr4:50958739-50958758 MsG0480020944.01.T01:CDS 30.0%
AATACAGAGAAGAGAAAGAA+AGG - Chr4:50957845-50957864 None:intergenic 30.0%
ACAACAACAACAAGATCATA+TGG + Chr4:50958737-50958756 MsG0480020944.01.T01:CDS 30.0%
ACCAACAACAATACTATTCT+TGG + Chr4:50959072-50959091 MsG0480020944.01.T01:CDS 30.0%
AGATATAGCTTCAAACTCTA+GGG + Chr4:50958965-50958984 MsG0480020944.01.T01:CDS 30.0%
ATACAGAGAAGAGAAAGAAA+GGG - Chr4:50957844-50957863 None:intergenic 30.0%
CAACAACAACAAGATCATAT+GGG + Chr4:50958738-50958757 MsG0480020944.01.T01:CDS 30.0%
CAATTAGTTCATGATGAGAA+GGG - Chr4:50958593-50958612 None:intergenic 30.0%
CTACTCATCTCTTATAACTT+TGG + Chr4:50959117-50959136 MsG0480020944.01.T01:exon 30.0%
CTGACGTTGTAATATCAATT+CGG + Chr4:50959270-50959289 MsG0480020944.01.T01:three_prime_UTR 30.0%
GAAGTAGCAAAATTTAGTGT+TGG - Chr4:50958376-50958395 None:intergenic 30.0%
TCCAAGAATAGTATTGTTGT+TGG - Chr4:50959076-50959095 None:intergenic 30.0%
TTCATATCTTTGATCCATTC+CGG - Chr4:50959143-50959162 None:intergenic 30.0%
TTGAATAGAAGAGAAAACCA+TGG - Chr4:50957946-50957965 None:intergenic 30.0%
! ATGGTTTTCTCTTCTATTCA+AGG + Chr4:50957945-50957964 MsG0480020944.01.T01:CDS 30.0%
!! AAAGTTTTGATGGCTCTTTA+GGG - Chr4:50957893-50957912 None:intergenic 30.0%
!! TAAAGTTTTGATGGCTCTTT+AGG - Chr4:50957894-50957913 None:intergenic 30.0%
!! TCTTGAGAGTTTGTTGAATT+AGG - Chr4:50958543-50958562 None:intergenic 30.0%
!!! ATTTTTAGGGTTTTTAGTGG+AGG - Chr4:50957714-50957733 None:intergenic 30.0%
AAAAGATTAAGGAAGAGAGG+AGG - Chr4:50957820-50957839 None:intergenic 35.0%
AAAGATTAAGGAAGAGAGGA+GGG - Chr4:50957819-50957838 None:intergenic 35.0%
ATGAGAGAATGAGGGAATAA+GGG + Chr4:50957752-50957771 MsG0480020944.01.T01:five_prime_UTR 35.0%
ATGGGGTTTCAAATTGGTAT+TGG + Chr4:50958756-50958775 MsG0480020944.01.T01:CDS 35.0%
CAAAAAGAGATGAGAGAATG+AGG + Chr4:50957743-50957762 MsG0480020944.01.T01:five_prime_UTR 35.0%
CCAATTAGTTCATGATGAGA+AGG - Chr4:50958594-50958613 None:intergenic 35.0%
CCTTCTCATCATGAACTAAT+TGG + Chr4:50958591-50958610 MsG0480020944.01.T01:CDS 35.0%
GAGATATAGCTTCAAACTCT+AGG + Chr4:50958964-50958983 MsG0480020944.01.T01:CDS 35.0%
GATATGAAGTTTCGAACGAT+TGG + Chr4:50959155-50959174 MsG0480020944.01.T01:three_prime_UTR 35.0%
GATCATATGGGGTTTCAAAT+TGG + Chr4:50958750-50958769 MsG0480020944.01.T01:CDS 35.0%
GGAAAAAGATTAAGGAAGAG+AGG - Chr4:50957823-50957842 None:intergenic 35.0%
GTTATGTAGATCCTCCTAAT+TGG + Chr4:50957967-50957986 MsG0480020944.01.T01:CDS 35.0%
GTTGTTGGAAACGTTAACAT+GGG + Chr4:50958681-50958700 MsG0480020944.01.T01:CDS 35.0%
TCATCTCTTATAACTTTGGC+CGG + Chr4:50959121-50959140 MsG0480020944.01.T01:exon 35.0%
TTGCTGCAGGTTAAAATAGA+TGG - Chr4:50958163-50958182 None:intergenic 35.0%
! ACTTTTGAATCAAACTCTGC+TGG + Chr4:50958879-50958898 MsG0480020944.01.T01:CDS 35.0%
! GTTGTTGTTGATGTTGTTCT+TGG - Chr4:50958725-50958744 None:intergenic 35.0%
!! CGGTTTTCTTCCATTTTAAC+CGG - Chr4:50959005-50959024 None:intergenic 35.0%
!! TTTTCTTCCATTTTAACCGG+TGG - Chr4:50959002-50959021 None:intergenic 35.0%
AATATTGAAGCTGCAGCATC+TGG + Chr4:50958933-50958952 MsG0480020944.01.T01:CDS 40.0%
AATCTGAGTCGTTACGTTGT+TGG + Chr4:50958666-50958685 MsG0480020944.01.T01:CDS 40.0%
AATTCCTAACCTACCCTTCA+TGG + Chr4:50958632-50958651 MsG0480020944.01.T01:CDS 40.0%
AATTGCTGCAAATTACGCCA+TGG - Chr4:50958846-50958865 None:intergenic 40.0%
CCAAATCATCAGCAAGCAAA+TGG + Chr4:50958183-50958202 MsG0480020944.01.T01:CDS 40.0%
CGTTGTTGGAAACGTTAACA+TGG + Chr4:50958680-50958699 MsG0480020944.01.T01:CDS 40.0%
CTTAGAAATGTTCCTGTTGG+TGG + Chr4:50958468-50958487 MsG0480020944.01.T01:CDS 40.0%
GATGAGAGAATGAGGGAATA+AGG + Chr4:50957751-50957770 MsG0480020944.01.T01:five_prime_UTR 40.0%
GCACTTAGAAATGTTCCTGT+TGG + Chr4:50958465-50958484 MsG0480020944.01.T01:CDS 40.0%
GGTAGGTTAGGAATTGTTTG+TGG - Chr4:50958627-50958646 None:intergenic 40.0%
TCTTATAACTTTGGCCGGAA+TGG + Chr4:50959126-50959145 MsG0480020944.01.T01:exon 40.0%
TTCTCTTCTCTGTATTCCCT+TGG + Chr4:50957849-50957868 MsG0480020944.01.T01:five_prime_UTR 40.0%
! ATGGTGGAGAATCAAACCAA+GGG - Chr4:50957868-50957887 None:intergenic 40.0%
! CCATTTGCTTGCTGATGATT+TGG - Chr4:50958186-50958205 None:intergenic 40.0%
AGAAGGGATTGCACATGATG+AGG - Chr4:50958577-50958596 None:intergenic 45.0%
GCAAGACATGTAGGCGTTAT+TGG + Chr4:50958430-50958449 MsG0480020944.01.T01:CDS 45.0%
GGAAACGTTAACATGGGTCT+TGG + Chr4:50958687-50958706 MsG0480020944.01.T01:CDS 45.0%
GTAGGCGTTATTGGACAAGA+GGG + Chr4:50958439-50958458 MsG0480020944.01.T01:CDS 45.0%
GTCACTTCTGCAAGACATGT+AGG + Chr4:50958421-50958440 MsG0480020944.01.T01:CDS 45.0%
TAGGCGTTATTGGACAAGAG+GGG + Chr4:50958440-50958459 MsG0480020944.01.T01:CDS 45.0%
TGTAGGCGTTATTGGACAAG+AGG + Chr4:50958438-50958457 MsG0480020944.01.T01:CDS 45.0%
! AGAAATGTTCCTGTTGGTGG+AGG + Chr4:50958471-50958490 MsG0480020944.01.T01:CDS 45.0%
! GAAATGTTCCTGTTGGTGGA+GGG + Chr4:50958472-50958491 MsG0480020944.01.T01:CDS 45.0%
! TCAAGAGCCACCGGTTAAAA+TGG + Chr4:50958992-50959011 MsG0480020944.01.T01:CDS 45.0%
! TTGATGGCTCTTTAGGGAGA+TGG - Chr4:50957887-50957906 None:intergenic 45.0%
! TTTACCTGGTGGTGCCAATT+AGG - Chr4:50957984-50958003 None:intergenic 45.0%
!! GATGGTGGAGAATCAAACCA+AGG - Chr4:50957869-50957888 None:intergenic 45.0%
!!! TTTGTTTTATTATATTTTTA+GGG - Chr4:50957727-50957746 None:intergenic 5.0%
!!! TTTTGTTTTATTATATTTTT+AGG - Chr4:50957728-50957747 None:intergenic 5.0%
ACGACTCAGATTCTGCATCG+AGG - Chr4:50958658-50958677 None:intergenic 50.0%
AGAAGTGACGAGGTTGAGTG+AGG - Chr4:50958410-50958429 None:intergenic 50.0%
AGGAGGAAAGCAGCAATGAG+AGG - Chr4:50957796-50957815 None:intergenic 50.0%
AGGCGTTATTGGACAAGAGG+GGG + Chr4:50958441-50958460 MsG0480020944.01.T01:CDS 50.0%
ATTCTGCATCGAGGCCATGA+AGG - Chr4:50958649-50958668 None:intergenic 50.0%
CAACGAGGACACTTTAGTGC+TGG - Chr4:50958345-50958364 None:intergenic 50.0%
CATGTCTTGCAGAAGTGACG+AGG - Chr4:50958420-50958439 None:intergenic 50.0%
CTCCCACTTGAGGTGGTAAA+GGG - Chr4:50958233-50958252 None:intergenic 50.0%
GATTAAGGAAGAGAGGAGGG+AGG - Chr4:50957816-50957835 None:intergenic 50.0%
GCTGCAGCATCTGGATTTGT+TGG + Chr4:50958942-50958961 MsG0480020944.01.T01:CDS 50.0%
GTTTCTGCTTCTGCTGTTGC+TGG + Chr4:50958804-50958823 MsG0480020944.01.T01:CDS 50.0%
TAAACGAGCTCGATCAGCCA+TGG - Chr4:50958301-50958320 None:intergenic 50.0%
TAGGGCTAATCAAGAGCCAC+CGG + Chr4:50958983-50959002 MsG0480020944.01.T01:CDS 50.0%
TCCCACTTGAGGTGGTAAAG+GGG - Chr4:50958232-50958251 None:intergenic 50.0%
TTCTGCATCGAGGCCATGAA+GGG - Chr4:50958648-50958667 None:intergenic 50.0%
! ATGGCTCTTTAGGGAGATGG+TGG - Chr4:50957884-50957903 None:intergenic 50.0%
!! ACTGGTGGTGGTGGTACTAT+GGG + Chr4:50958258-50958277 MsG0480020944.01.T01:CDS 50.0%
!! CACTTTAGTGCTGGTTCTGG+TGG - Chr4:50958336-50958355 None:intergenic 50.0%
!! GAGGTGTTGGAGTTGAACCA+TGG + Chr4:50958826-50958845 MsG0480020944.01.T01:CDS 50.0%
!! GCTGATGATTTGGTTGCTGC+AGG - Chr4:50958176-50958195 None:intergenic 50.0%
!! GGACACTTTAGTGCTGGTTC+TGG - Chr4:50958339-50958358 None:intergenic 50.0%
!! GTGTTGGTTGAATCGCAACG+AGG - Chr4:50958360-50958379 None:intergenic 50.0%
!! TACTGGTGGTGGTGGTACTA+TGG + Chr4:50958257-50958276 MsG0480020944.01.T01:CDS 50.0%
!! TTTAGTGCTGGTTCTGGTGG+TGG - Chr4:50958333-50958352 None:intergenic 50.0%
ACTATGGGCTCGATCAGACC+AGG + Chr4:50958273-50958292 MsG0480020944.01.T01:CDS 55.0%
CCAGTACTACCTCCCACTTG+AGG - Chr4:50958243-50958262 None:intergenic 55.0%
CCCACTTGAGGTGGTAAAGG+GGG - Chr4:50958231-50958250 None:intergenic 55.0%
CCCCCTTTACCACCTCAAGT+GGG + Chr4:50958228-50958247 MsG0480020944.01.T01:CDS 55.0%
CCTCAAGTGGGAGGTAGTAC+TGG + Chr4:50958240-50958259 MsG0480020944.01.T01:CDS 55.0%
CCTCCCACTTGAGGTGGTAA+AGG - Chr4:50958234-50958253 None:intergenic 55.0%
CCTTTACCACCTCAAGTGGG+AGG + Chr4:50958231-50958250 MsG0480020944.01.T01:CDS 55.0%
GAGGCCATGAAGGGTAGGTT+AGG - Chr4:50958639-50958658 None:intergenic 55.0%
GTACTACCTCCCACTTGAGG+TGG - Chr4:50958240-50958259 None:intergenic 55.0%
TAAGGAAGAGAGGAGGGAGG+AGG - Chr4:50957813-50957832 None:intergenic 55.0%
TCCCCCTTTACCACCTCAAG+TGG + Chr4:50958227-50958246 MsG0480020944.01.T01:CDS 55.0%
TCCTCCTAATTGGCACCACC+AGG + Chr4:50957977-50957996 MsG0480020944.01.T01:CDS 55.0%
TCTGCTTCTGCTGTTGCTGG+AGG + Chr4:50958807-50958826 MsG0480020944.01.T01:CDS 55.0%
! ACCTGGTGGTGCCAATTAGG+AGG - Chr4:50957981-50958000 None:intergenic 55.0%
! CAAGTGGGAGGTAGTACTGG+TGG + Chr4:50958243-50958262 MsG0480020944.01.T01:CDS 55.0%
! TCTGCTGTTGCTGGAGGTGT+TGG + Chr4:50958813-50958832 MsG0480020944.01.T01:CDS 55.0%
CTACGACACCCTCCACCAAC+AGG - Chr4:50958483-50958502 None:intergenic 60.0%
CTCGATCAGACCAGGCTCCA+TGG + Chr4:50958281-50958300 MsG0480020944.01.T01:CDS 60.0%
GCATCGAGGCCATGAAGGGT+AGG - Chr4:50958644-50958663 None:intergenic 60.0%
!! GGAGGTAGTACTGGTGGTGG+TGG + Chr4:50958249-50958268 MsG0480020944.01.T01:CDS 60.0%
!! GTGGGAGGTAGTACTGGTGG+TGG + Chr4:50958246-50958265 MsG0480020944.01.T01:CDS 60.0%
GCTCGATCAGCCATGGAGCC+TGG - Chr4:50958294-50958313 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
Chr4 gene 50957697 50959323 50957697 ID=MsG0480020944.01;Name=MsG0480020944.01
Chr4 mRNA 50957697 50959323 50957697 ID=MsG0480020944.01.T01;Parent=MsG0480020944.01;Name=MsG0480020944.01.T01;_AED=0.06;_eAED=0.06;_QI=248|1|1|1|1|1|2|190|335
Chr4 exon 50957697 50957998 50957697 ID=MsG0480020944.01.T01:exon:358;Parent=MsG0480020944.01.T01
Chr4 exon 50958180 50959323 50958180 ID=MsG0480020944.01.T01:exon:359;Parent=MsG0480020944.01.T01
Chr4 five_prime_UTR 50957697 50957944 50957697 ID=MsG0480020944.01.T01:five_prime_utr;Parent=MsG0480020944.01.T01
Chr4 CDS 50957945 50957998 50957945 ID=MsG0480020944.01.T01:cds;Parent=MsG0480020944.01.T01
Chr4 CDS 50958180 50959133 50958180 ID=MsG0480020944.01.T01:cds;Parent=MsG0480020944.01.T01
Chr4 three_prime_UTR 50959134 50959323 50959134 ID=MsG0480020944.01.T01:three_prime_utr;Parent=MsG0480020944.01.T01
Gene Sequence

>MsG0480020944.01.T01

ATGGTTTTCTCTTCTATTCAAGGTTATGTAGATCCTCCTAATTGGCACCACCAGCAACCAAATCATCAGCAAGCAAATGGAAGCGATAACACTCAACTTCTTCCCCCTTTACCACCTCAAGTGGGAGGTAGTACTGGTGGTGGTGGTACTATGGGCTCGATCAGACCAGGCTCCATGGCTGATCGAGCTCGTTTAGCAAAACTACCACCACCAGAACCAGCACTAAAGTGTCCTCGTTGCGATTCAACCAACACTAAATTTTGCTACTTCAACAACTACAGCCTCACTCAACCTCGTCACTTCTGCAAGACATGTAGGCGTTATTGGACAAGAGGGGGTGCACTTAGAAATGTTCCTGTTGGTGGAGGGTGTCGTAGAAACAACAAGAAAAACAAAAGAAGCCGTTCAAAATCTCCTAATTCAACAAACTCTCAAGAAAAACAAACTTCCTCATCATGTGCAATCCCTTCTCATCATGAACTAATTGGTCATCAGTTACCACAAACAATTCCTAACCTACCCTTCATGGCCTCGATGCAGAATCTGAGTCGTTACGTTGTTGGAAACGTTAACATGGGTCTTGGTTTGCGTGAGATCCAAGAACAACATCAACAACAACAACAAGATCATATGGGGTTTCAAATTGGTATTGGTAATGGTAACTCAACTTCTGTTTCTGTTTCTGCTTCTGCTGTTGCTGGAGGTGTTGGAGTTGAACCATGGCGTAATTTGCAGCAATTTCCTTTCTTGAATACTTTTGAATCAAACTCTGCTGGAAATAATTCATATCCTTTTCAAGGAGAAAGTAATATTGAAGCTGCAGCATCTGGATTTGTTGGAGATATAGCTTCAAACTCTAGGGCTAATCAAGAGCCACCGGTTAAAATGGAAGAAAACCGCGCTTTAAATTTATCAAGAACATCTCTTGGTGTCTCTGAACATAATAACCAACAACAATACTATTCTTGGAATGATTTATCAGGTTCTTCAACTACTCATCTCTTATAA
Protein sequence

>MsG0480020944.01.T01

MVFSSIQGYVDPPNWHHQQPNHQQANGSDNTQLLPPLPPQVGGSTGGGGTMGSIRPGSMADRARLAKLPPPEPALKCPRCDSTNTKFCYFNNYSLTQPRHFCKTCRRYWTRGGALRNVPVGGGCRRNNKKNKRSRSKSPNSTNSQEKQTSSSCAIPSHHELIGHQLPQTIPNLPFMASMQNLSRYVVGNVNMGLGLREIQEQHQQQQQDHMGFQIGIGNGNSTSVSVSASAVAGGVGVEPWRNLQQFPFLNTFESNSAGNNSYPFQGESNIEAAASGFVGDIASNSRANQEPPVKMEENRALNLSRTSLGVSEHNNQQQYYSWNDLSGSSTTHLL*