AlfalfaGEDB | MsG0480021012.01

M. sativa cultivar ZhongmuNo.1 / MsG0480021012.01

KEGG: -
GO: GO:0004672 GO:0005524 GO:0006468
PFAM: PF07714

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480021012.01.T01	XP_024637586.1	95.11	409	5	1	1	394	45	453	0	803

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480021012.01.T01	Q9SF86	61.812	309	102	2	29	322	65	372	1.29E-134	394

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480021012.01.T01	A0A396I8B4	95.110	409	5	1	1	394	45	453	0.0	803

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
MsG0480018334.01	MsG0480021012.01	PPI
MsG0480021012.01	MsG0780040233.01	PPI
MsG0480021012.01	MsG0880047337.01	PPI
MsG0480021012.01	MsG0280007018.01	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480021012.01.T01	MTR_4g063950	95.297	404	9	1	1	394	45	448	0.0	796
MsG0480021012.01.T01	MTR_1g063910	55.319	376	142	6	2	353	38	411	3.78e-140	406
MsG0480021012.01.T01	MTR_1g063910	55.319	376	142	6	2	353	47	420	5.69e-140	406
MsG0480021012.01.T01	MTR_7g101800	56.105	344	127	4	1	322	33	374	1.17e-136	397
MsG0480021012.01.T01	MTR_7g101800	56.105	344	127	4	1	322	41	382	1.82e-136	397
MsG0480021012.01.T01	MTR_7g101800	56.105	344	127	4	1	322	53	394	2.08e-136	397
MsG0480021012.01.T01	MTR_8g008820	57.827	313	116	2	25	322	53	364	2.09e-120	356
MsG0480021012.01.T01	MTR_4g099130	55.556	306	120	2	25	315	46	350	2.33e-115	341
MsG0480021012.01.T01	MTR_5g013610	54.072	307	122	4	25	315	41	344	3.96e-110	327
MsG0480021012.01.T01	MTR_8g469230	51.803	305	122	4	30	315	114	412	1.22e-98	302

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480021012.01.T01	AT2G28940	65.707	417	113	8	3	394	51	462	0.0	524
MsG0480021012.01.T01	AT2G28940	71.519	316	83	5	81	394	33	343	3.03e-160	454
MsG0480021012.01.T01	AT3G09830	61.812	309	102	2	29	322	65	372	1.31e-135	394
MsG0480021012.01.T01	AT3G09830	61.812	309	102	2	29	322	65	372	1.31e-135	394
MsG0480021012.01.T01	AT3G09830	61.812	309	102	2	29	322	65	372	1.31e-135	394
MsG0480021012.01.T01	AT5G03320	57.558	344	124	4	2	324	29	371	1.80e-132	386
MsG0480021012.01.T01	AT5G03320	57.558	344	124	4	2	324	29	371	1.80e-132	386
MsG0480021012.01.T01	AT2G39110	60.703	313	108	1	25	322	67	379	1.54e-128	377
MsG0480021012.01.T01	AT2G39110	60.703	313	108	1	25	322	52	364	2.06e-128	376
MsG0480021012.01.T01	AT5G47070	49.459	370	159	8	3	352	38	399	8.31e-106	318

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Find 88 sgRNAs with CRISPR-Local

Find 161 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACATAGCCAAAGCCTTCTG+CGG	0.598757	4:+52051031	None:intergenic
AACCTGAACCAGCTAAACAG+GGG	0.710672	4:-52050274	MsG0480021012.01.T01:CDS
AAGAGGGATTCAGAGGCTTT+TGG	0.356513	4:-52052089	MsG0480021012.01.T01:CDS
AAGAGTGATGTGTGGAGCTT+TGG	0.430091	4:-52050650	MsG0480021012.01.T01:CDS
AAGATAAGCCAACCCACGAG+CGG	0.673322	4:+52051964	None:intergenic
AAGATTTGATTGGAAGAGTT+GGG	0.443023	4:-52050186	MsG0480021012.01.T01:CDS
AATCCATTGGAAGATTTGAT+TGG	0.351673	4:-52050196	MsG0480021012.01.T01:CDS
AATCGGAATCGTATTCGGAG+CGG	0.728176	4:+52052553	None:intergenic
AATGCAAAGCTTTCTGATTT+TGG	0.064179	4:-52051071	MsG0480021012.01.T01:CDS
ACAAAGGTTGTTGGCACCAT+AGG	0.470699	4:-52050716	MsG0480021012.01.T01:intron

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAAAACGAACAAAAGCTTC+TGG	-	Chr4:52052151-52052170	MsG0480021012.01.T01:intron	30.0%
AAAATCCGGATTACAATCTA+AGG	+	Chr4:52051951-52051970	None:intergenic	30.0%
AAGATTTGATTGGAAGAGTT+GGG	-	Chr4:52052538-52052557	MsG0480021012.01.T01:CDS	30.0%
AATCCATTGGAAGATTTGAT+TGG	-	Chr4:52052528-52052547	MsG0480021012.01.T01:CDS	30.0%
ACAACTATGCCATCTTAAAA+GGG	+	Chr4:52051195-52051214	None:intergenic	30.0%
ATCAAACAGTTGAATCGTAA+TGG	-	Chr4:52050400-52050419	MsG0480021012.01.T01:CDS	30.0%
GTGATGATTATGATGAACTA+GGG	+	Chr4:52050359-52050378	None:intergenic	30.0%
TCACATATCAGTAGAAAATC+CGG	+	Chr4:52051965-52051984	None:intergenic	30.0%
TTCTTTGGATCAGATACATA+TGG	+	Chr4:52052188-52052207	None:intergenic	30.0%
AAGATGGCATAGTTGTGTTT+AGG	-	Chr4:52051199-52051218	MsG0480021012.01.T01:intron	35.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr4	gene	52050160	52052586	52050160	ID=MsG0480021012.01;Name=MsG0480021012.01
Chr4	mRNA	52050160	52052586	52050160	ID=MsG0480021012.01.T01;Parent=MsG0480021012.01;Name=MsG0480021012.01.T01;_AED=0.38;_eAED=0.42;_QI=0\|0\|0\|1\|1\|1\|4\|0\|394
Chr4	exon	52052325	52052586	52052325	ID=MsG0480021012.01.T01:exon:759;Parent=MsG0480021012.01.T01
Chr4	exon	52051943	52052169	52051943	ID=MsG0480021012.01.T01:exon:758;Parent=MsG0480021012.01.T01
Chr4	exon	52051022	52051144	52051022	ID=MsG0480021012.01.T01:exon:757;Parent=MsG0480021012.01.T01
Chr4	exon	52050160	52050732	52050160	ID=MsG0480021012.01.T01:exon:756;Parent=MsG0480021012.01.T01
Chr4	CDS	52052325	52052586	52052325	ID=MsG0480021012.01.T01:cds;Parent=MsG0480021012.01.T01
Chr4	CDS	52051943	52052169	52051943	ID=MsG0480021012.01.T01:cds;Parent=MsG0480021012.01.T01
Chr4	CDS	52051022	52051144	52051022	ID=MsG0480021012.01.T01:cds;Parent=MsG0480021012.01.T01
Chr4	CDS	52050160	52050732	52050160	ID=MsG0480021012.01.T01:cds;Parent=MsG0480021012.01.T01

Gene Sequence

>MsG0480021012.01.T01

ATGGACACGCGCCGCTCCGAATACGATTCCGATTCTAGAGACTTCTCCGACACGCTTGGCTTCCATGAGTTTCTCTCTCAACGCCGTGCTAATCATCTTCATCTGTTTTCATTCTCTGACCTCAAAATCGCTACTCGAGGATTCAATAGAGCGTTGTTAATTGGTGAAGGTGGATTTGGTTCGGTTTATAGAGGAACCCTAGTTCATCATAATCATCACCGTTCTCAAATCGATGTTGCTATCAAACAGTTGAATCGTAATGGGGTGATTAAGCATCCAAATTTGGTGAGGTTAGTAGGTTATTGTGCTGAAGATGATGAAAGAGGGATTCAGAGGCTTTTGGTGTATGAGTTTATGTCTAATAAGAGTTTGGAAGATCATCTTTTGGTTCGTGTTCCTTCGTCAGTTCTTCCTTGGATTACTAGATTAAGAATTGCTCAAGATGCCGCTCGTGGGTTGGCTTATCTTCATGAGGAAATGGATTTTCAGCTAATATTTCGAGATTTTAAAACATCCAACATCTTGCTGGATGAGGACTTCAATGCAAAGCTTTCTGATTTTGGACTCGCTAGGCAAGGACCCGCAGAAGGCTTTGGCTATGTTTCTACAGCGGTTGTTGGCACCATAGGATATGCTGCACCAGAGTATGTTCATACCGGAAAGCTGACTGCTAAGAGTGATGTGTGGAGCTTTGGTGTAGTTCTTTACGAGCTTATCACTGGGAGGAGAGCCGTAGAAAGAAACCTACCAAAAAACGAACAAAAGCTTCTGGATTGGGTAAGACCATATGTATCTGATCCAAAGAAGTTTCATCATATTGTCGATCCTCGACTTGAGGCACAGGATTGCACCAAATCAGCTCATAAACTTGCAGTCCTGGCAAACAAGTGTCTCATGAAGCAGCCAAAGTCTCGCCCAAAAATGAGTGAGGTGGTTGAGATTCTTGGAAACATTATTGACGAGTCTGTTCCCTTGGATGAATGCATTTCCGAAGCTGTTGCAGAAACAGGAGAAGCAAATGAAGTAAATTTATCTCTAGAGAATGCTCAACCTGAACCAGCTAAACAGGGGAATAATTATCTGAAGAAGGTTTTTGAATTCAGAGACAAGGTGAGTTTAAGAAATAAATCCATTGGAAGATTTGATTGGAAGAGTTGGGCTCCTGGGCTTGTAAGAACATGGTGA

Protein sequence

>MsG0480021012.01.T01

MDTRRSEYDSDSRDFSDTLGFHEFLSQRRANHLHLFSFSDLKIATRGFNRALLIGEGGFGSVYRGTLVHHNHHRSQIDVAIKQLNRNGVIKHPNLVRLVGYCAEDDERGIQRLLVYEFMSNKSLEDHLLVRVPSSVLPWITRLRIAQDAARGLAYLHEEMDFQLIFRDFKTSNILLDEDFNAKLSDFGLARQGPAEGFGYVSTAVVGTIGYAAPEYVHTGKLTAKSDVWSFGVVLYELITGRRAVERNLPKNEQKLLDWVRPYVSDPKKFHHIVDPRLEAQDCTKSAHKLAVLANKCLMKQPKSRPKMSEVVEILGNIIDESVPLDECISEAVAETGEANEVNLSLENAQPEPAKQGNNYLKKVFEFRDKVSLRNKSIGRFDWKSWAPGLVRTW*