Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480021036.01.T01 | XP_003606718.1 | 74.825 | 286 | 63 | 4 | 15 | 296 | 1 | 281 | 7.44E-139 | 417 |
| MsG0480021036.01.T01 | XP_003606718.1 | 36.842 | 361 | 158 | 6 | 62 | 355 | 151 | 508 | 2.08E-51 | 191 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180003188.01 | MsG0480021036.01 | 0.819050 | 1.423565e-52 | 1.083781e-49 |
| MsG0280007164.01 | MsG0480021036.01 | 0.803263 | 3.790019e-49 | 1.895970e-46 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480021036.01.T01 | MTR_4g064770 | 74.825 | 286 | 63 | 4 | 15 | 296 | 1 | 281 | 9.01e-143 | 417 |
| MsG0480021036.01.T01 | MTR_4g064770 | 36.842 | 361 | 158 | 6 | 62 | 355 | 151 | 508 | 2.52e-55 | 191 |
| MsG0480021036.01.T01 | MTR_2g101710 | 32.250 | 400 | 174 | 7 | 46 | 350 | 50 | 447 | 4.44e-49 | 175 |
| MsG0480021036.01.T01 | MTR_3g063220 | 28.148 | 405 | 196 | 6 | 39 | 350 | 107 | 509 | 3.63e-45 | 163 |
| MsG0480021036.01.T01 | MTR_2g036960 | 31.900 | 279 | 149 | 3 | 82 | 330 | 156 | 423 | 8.95e-45 | 164 |
| MsG0480021036.01.T01 | MTR_2g036960 | 29.066 | 289 | 161 | 10 | 59 | 313 | 235 | 513 | 1.49e-26 | 112 |
| MsG0480021036.01.T01 | MTR_2g036960 | 28.889 | 270 | 149 | 8 | 63 | 297 | 38 | 299 | 1.96e-25 | 108 |
| MsG0480021036.01.T01 | MTR_1g059280 | 29.592 | 294 | 170 | 4 | 34 | 297 | 36 | 322 | 4.01e-43 | 159 |
| MsG0480021036.01.T01 | MTR_1g112500 | 32.192 | 292 | 167 | 3 | 36 | 297 | 5 | 295 | 4.07e-38 | 145 |
| MsG0480021036.01.T01 | MTR_1g112500 | 24.936 | 393 | 193 | 9 | 57 | 350 | 229 | 618 | 1.34e-20 | 94.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480021036.01.T01 | AT5G59600 | 47.514 | 181 | 93 | 2 | 45 | 225 | 18 | 196 | 5.14e-47 | 168 |
| MsG0480021036.01.T01 | AT5G59600 | 31.268 | 355 | 175 | 7 | 62 | 350 | 136 | 487 | 5.79e-35 | 135 |
| MsG0480021036.01.T01 | AT5G59600 | 31.944 | 144 | 68 | 2 | 183 | 296 | 53 | 196 | 1.57e-14 | 75.5 |
| MsG0480021036.01.T01 | AT4G18520 | 30.205 | 341 | 196 | 7 | 32 | 340 | 274 | 604 | 1.26e-43 | 160 |
| MsG0480021036.01.T01 | AT4G18520 | 29.304 | 273 | 160 | 4 | 55 | 297 | 196 | 465 | 4.47e-34 | 133 |
| MsG0480021036.01.T01 | AT4G18520 | 28.223 | 287 | 174 | 5 | 41 | 297 | 80 | 364 | 1.18e-26 | 112 |
| MsG0480021036.01.T01 | AT3G13770 | 29.283 | 321 | 185 | 4 | 46 | 326 | 55 | 373 | 8.68e-43 | 157 |
| MsG0480021036.01.T01 | AT3G13770 | 30.000 | 250 | 169 | 4 | 45 | 292 | 155 | 400 | 6.07e-28 | 115 |
| MsG0480021036.01.T01 | AT5G55740 | 35.135 | 296 | 158 | 4 | 34 | 297 | 61 | 354 | 6.30e-42 | 157 |
| MsG0480021036.01.T01 | AT5G55740 | 29.699 | 266 | 155 | 4 | 62 | 297 | 192 | 455 | 1.58e-31 | 127 |
Find 94 sgRNAs with CRISPR-Local
Find 152 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAATCTCGAGAGCGTGATCA+TGG | 0.475577 | 4:+52459245 | None:intergenic |
| AAGGGCTTCGAATGAGGCGT+TGG | 0.473182 | 4:-52458943 | MsG0480021036.01.T01:CDS |
| AATGGGTGTGAAGGATTTGG+TGG | 0.535507 | 4:-52458999 | MsG0480021036.01.T01:CDS |
| ACGTAAGGTGTTTGATGGAA+TGG | 0.522286 | 4:-52459017 | MsG0480021036.01.T01:CDS |
| ACTCTAAGTGCGGTGAAGTT+AGG | 0.334128 | 4:-52459045 | MsG0480021036.01.T01:CDS |
| ACTGAGGTAAGAGTGAGGTT+GGG | 0.579888 | 4:-52458637 | MsG0480021036.01.T01:intron |
| ACTGGAGCTTTATGCATGCA+TGG | 0.652467 | 4:-52458262 | MsG0480021036.01.T01:CDS |
| AGACGATTCCCAGCACTCTC+AGG | 0.461506 | 4:+52458077 | None:intergenic |
| AGAGTTAGACCTGAGAGTGC+TGG | 0.481479 | 4:-52458086 | MsG0480021036.01.T01:CDS |
| AGATTATGCAAGAGAAATAC+TGG | 0.395684 | 4:-52458297 | MsG0480021036.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AGATTATGCAAGAGAAATAC+TGG | - | Chr4:52459313-52459332 | MsG0480021036.01.T01:CDS | 30.0% | |
| ATCAAGAGAAGGATAAAGAA+AGG | - | Chr4:52459607-52459626 | MsG0480021036.01.T01:CDS | 30.0% | |
| ATGTTGGAGATATTGAACTT+GGG | - | Chr4:52459277-52459296 | MsG0480021036.01.T01:CDS | 30.0% | |
| ATTGTTATGTACTCTAAGTG+CGG | - | Chr4:52458555-52458574 | MsG0480021036.01.T01:CDS | 30.0% | |
| GTTGATATGTATGCAAAATG+TGG | - | Chr4:52459062-52459081 | MsG0480021036.01.T01:CDS | 30.0% | |
| TCAAGAGAAGGATAAAGAAA+GGG | - | Chr4:52459608-52459627 | MsG0480021036.01.T01:CDS | 30.0% | |
| ACTTGGAATGCTTTGATATC+TGG | - | Chr4:52458736-52458755 | MsG0480021036.01.T01:intron | 35.0% | |
| AGGTTGTGTATAAGGTAATG+AGG | + | Chr4:52458245-52458264 | None:intergenic | 35.0% | |
| ATTACCTTATACACAACCTG+TGG | - | Chr4:52458246-52458265 | MsG0480021036.01.T01:CDS | 35.0% | |
| CAACGGTGAATATTTGTTTG+GGG | + | Chr4:52458309-52458328 | None:intergenic | 35.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 52457994 | 52459638 | 52457994 | ID=MsG0480021036.01;Name=MsG0480021036.01 |
| Chr4 | mRNA | 52457994 | 52459638 | 52457994 | ID=MsG0480021036.01.T01;Parent=MsG0480021036.01;Name=MsG0480021036.01.T01;_AED=0.49;_eAED=0.58;_QI=0|0|0|1|1|1|4|0|384 |
| Chr4 | exon | 52458943 | 52459638 | 52458943 | ID=MsG0480021036.01.T01:exon:831;Parent=MsG0480021036.01.T01 |
| Chr4 | exon | 52458455 | 52458648 | 52458455 | ID=MsG0480021036.01.T01:exon:830;Parent=MsG0480021036.01.T01 |
| Chr4 | exon | 52458228 | 52458329 | 52458228 | ID=MsG0480021036.01.T01:exon:829;Parent=MsG0480021036.01.T01 |
| Chr4 | exon | 52457994 | 52458156 | 52457994 | ID=MsG0480021036.01.T01:exon:828;Parent=MsG0480021036.01.T01 |
| Chr4 | CDS | 52458943 | 52459638 | 52458943 | ID=MsG0480021036.01.T01:cds;Parent=MsG0480021036.01.T01 |
| Chr4 | CDS | 52458455 | 52458648 | 52458455 | ID=MsG0480021036.01.T01:cds;Parent=MsG0480021036.01.T01 |
| Chr4 | CDS | 52458228 | 52458329 | 52458228 | ID=MsG0480021036.01.T01:cds;Parent=MsG0480021036.01.T01 |
| Chr4 | CDS | 52457994 | 52458156 | 52457994 | ID=MsG0480021036.01.T01:cds;Parent=MsG0480021036.01.T01 |
| Gene Sequence |
>MsG0480021036.01.T01 ATATTTGGCGCTCGTAATGCTCTCAGTGGCTTAGTGCCTACTATGCATGCTCTTGTAAGAACTTCACCATCCCTCAGAATTCCCATAAAAAATGCCATCTTCAACCACCGTTTTCTCCGATCAGAACCCGAATCCTACACAAAACTCATCGAAACATACATTCGCTCACGATCATTACATCAAGGAAAGAAGCTTCATGCGCTTCTAACAACCAACGGTTACGCGCGTTTCAACCTCATAGCATCCAACCTCATTACCTTATACACAACCTGTGGCCAACTCTCCATTGCACGTAAACTGTTCGACAAAATTCCCCAAACAAATATTCACCGTTGGATTGCCCTCATTGCCACATGTGCTCGCTGTGGCTTCCATGATCACGCTCTCGAGATTTTCTCTGAAATGCAAAGTCTCAATGGCCAAAAGTCCAATAGCGTATTCGTCATTCCTAGTGTTCTTAAAGCTTGTGGCCATGTTGGTGATAGAATTTACGGAGAGCAAGTGCATTGTTTGGTTTTGAAGTGTTCGTTTGAGATTGATGCTTTTGTTTCGAGTGCGTTGATTGTTATGTACTCTAAGTGCGGTGAAGTTAGGGATGCACGTAAGGTGTTTGATGGAATGGGTGTGAAGGATTTGGTGGCAATGAATGCTGTTGTTTCTGGGTATGCTCAACAAGGGCTTCGAATGAGGCGTTGGAGTGAGGTTGGGAAGGAGATTCATGGTTATGCTTTGGTGATTGGTGTGGAGGATGATTTGTATGTGAGGAGTGCTTTGGTTGATATGTATGCAAAATGTGGGTTCATTTCGGAAGCCAGGACACTGTTTTATAAGATGCCGGAGAAGAACACTGTGACAATGAACTCTATGATTTTTGGTTATGCAAATCATGGAGGCTGTTCAAGATTATGCAAGAGAAATACTGGAGTGAGCCACAGACTGGAGCTTTATGCATGCATGGTGGATCGTTTTGGTCGAGCGAAAACTAGAGAAGCAAATCATGGGCATGTGGAGCTTGCAGAAGTGGCTGCTAAGCATTTGTCAGAGTTAGACCTGAGAGTGCTGGGAATCGTCTTCTGTTGTCCGCTTGTATGCCGATGCTGGAACATGGGGAAAGGTTTAGCGGATCAAGAGAAGGATAAAGAAAGGGAAACTTAG |
| Protein sequence |
>MsG0480021036.01.T01 IFGARNALSGLVPTMHALVRTSPSLRIPIKNAIFNHRFLRSEPESYTKLIETYIRSRSLHQGKKLHALLTTNGYARFNLIASNLITLYTTCGQLSIARKLFDKIPQTNIHRWIALIATCARCGFHDHALEIFSEMQSLNGQKSNSVFVIPSVLKACGHVGDRIYGEQVHCLVLKCSFEIDAFVSSALIVMYSKCGEVRDARKVFDGMGVKDLVAMNAVVSGYAQQGLRMRRWSEVGKEIHGYALVIGVEDDLYVRSALVDMYAKCGFISEARTLFYKMPEKNTVTMNSMIFGYANHGGCSRLCKRNTGVSHRLELYACMVDRFGRAKTREANHGHVELAEVAAKHLSELDLRVLGIVFCCPLVCRCWNMGKGLADQEKDKERET* |
