Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280007164.01.T01 | ABN09753.1 | 94.157 | 599 | 35 | 0 | 1 | 599 | 97 | 695 | 0 | 1179 |
| MsG0280007164.01.T01 | ABN09753.1 | 28.226 | 496 | 325 | 4 | 88 | 553 | 52 | 546 | 1.20E-61 | 228 |
| MsG0280007164.01.T01 | ABN09753.1 | 27.469 | 324 | 184 | 5 | 217 | 534 | 49 | 327 | 2.53E-24 | 120 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080047869.01 | MsG0280007164.01 | 0.829832 | 4.129021e-55 | 4.275555e-52 |
| MsG0080047957.01 | MsG0280007164.01 | 0.800477 | 1.415010e-48 | 6.595053e-46 |
| MsG0080047959.01 | MsG0280007164.01 | 0.809767 | 1.608298e-50 | 9.526060e-48 |
| MsG0080048683.01 | MsG0280007164.01 | 0.804193 | 2.429793e-49 | 1.244905e-46 |
| MsG0080048785.01 | MsG0280007164.01 | 0.813390 | 2.621994e-51 | 1.710144e-48 |
| MsG0080048856.01 | MsG0280007164.01 | 0.857832 | 1.247504e-62 | 3.119953e-59 |
| MsG0080048892.01 | MsG0280007164.01 | 0.828271 | 9.865900e-55 | 9.758451e-52 |
| MsG0080048916.01 | MsG0280007164.01 | 0.827432 | 1.569658e-54 | 1.515151e-51 |
| MsG0080048926.01 | MsG0280007164.01 | 0.807074 | 6.035884e-50 | 3.330141e-47 |
| MsG0080049148.01 | MsG0280007164.01 | 0.870443 | 1.453542e-66 | 5.655308e-63 |
PPI
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280007164.01.T01 | MTR_2g021710 | 94.157 | 599 | 35 | 0 | 1 | 599 | 97 | 695 | 0.0 | 1179 |
| MsG0280007164.01.T01 | MTR_2g021710 | 28.226 | 496 | 325 | 4 | 88 | 553 | 52 | 546 | 1.46e-65 | 228 |
| MsG0280007164.01.T01 | MTR_2g021710 | 27.469 | 324 | 184 | 5 | 217 | 534 | 49 | 327 | 3.06e-28 | 120 |
| MsG0280007164.01.T01 | MTR_1g103840 | 37.209 | 602 | 368 | 4 | 1 | 596 | 54 | 651 | 8.89e-144 | 436 |
| MsG0280007164.01.T01 | MTR_1g103840 | 26.908 | 498 | 322 | 7 | 86 | 548 | 7 | 497 | 6.91e-57 | 205 |
| MsG0280007164.01.T01 | MTR_3g052720 | 36.452 | 620 | 357 | 5 | 11 | 596 | 65 | 681 | 6.69e-120 | 382 |
| MsG0280007164.01.T01 | MTR_3g052720 | 29.385 | 439 | 277 | 3 | 2 | 439 | 221 | 627 | 2.59e-58 | 212 |
| MsG0280007164.01.T01 | MTR_3g052720 | 26.012 | 346 | 194 | 4 | 1 | 345 | 352 | 636 | 2.37e-33 | 137 |
| MsG0280007164.01.T01 | MTR_1g073300 | 34.032 | 620 | 381 | 11 | 1 | 596 | 49 | 664 | 6.97e-117 | 366 |
| MsG0280007164.01.T01 | MTR_1g073300 | 25.103 | 486 | 303 | 13 | 109 | 539 | 25 | 504 | 1.79e-36 | 145 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280007164.01.T01 | AT5G19020 | 59.235 | 601 | 243 | 2 | 1 | 599 | 85 | 685 | 0.0 | 752 |
| MsG0280007164.01.T01 | AT5G19020 | 26.033 | 484 | 325 | 5 | 92 | 543 | 44 | 526 | 2.27e-52 | 191 |
| MsG0280007164.01.T01 | AT5G19020 | 25.134 | 374 | 216 | 10 | 220 | 532 | 40 | 410 | 1.70e-26 | 115 |
| MsG0280007164.01.T01 | AT2G22070 | 37.062 | 599 | 373 | 4 | 1 | 596 | 58 | 655 | 3.77e-134 | 410 |
| MsG0280007164.01.T01 | AT2G22070 | 27.733 | 494 | 314 | 6 | 87 | 539 | 4 | 495 | 4.94e-55 | 200 |
| MsG0280007164.01.T01 | AT1G08070 | 39.734 | 526 | 282 | 3 | 73 | 596 | 14 | 506 | 2.53e-126 | 386 |
| MsG0280007164.01.T01 | AT1G08070 | 29.787 | 376 | 198 | 5 | 1 | 373 | 74 | 386 | 2.91e-46 | 173 |
| MsG0280007164.01.T01 | AT1G08070 | 27.530 | 247 | 147 | 5 | 324 | 541 | 1 | 244 | 1.23e-21 | 99.8 |
| MsG0280007164.01.T01 | AT1G08070 | 38.710 | 558 | 307 | 3 | 42 | 597 | 87 | 611 | 4.70e-126 | 388 |
| MsG0280007164.01.T01 | AT1G08070 | 29.787 | 376 | 198 | 5 | 1 | 373 | 178 | 490 | 9.25e-46 | 173 |
Find 145 sgRNAs with CRISPR-Local
Find 166 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAACCATCAAACAACAGCT+GGG | 0.645382 | 2:+11307026 | None:intergenic |
| AAACTGTTTGATGTAATGCC+CGG | 0.551700 | 2:-11306928 | MsG0280007164.01.T01:CDS |
| AAATGTTCACATAGGAGAGA+GGG | 0.610808 | 2:-11305444 | MsG0280007164.01.T01:CDS |
| AAGAAGCTGAGGAAATTATA+AGG | 0.386498 | 2:-11305532 | MsG0280007164.01.T01:CDS |
| AATATCGGCCTCCATTGGCA+TGG | 0.643435 | 2:+11305508 | None:intergenic |
| AATGAAAAGCGCACATAATG+TGG | 0.535338 | 2:-11305615 | MsG0280007164.01.T01:CDS |
| AATGCCCAGCTGTTGTTTGA+TGG | 0.376877 | 2:-11307030 | MsG0280007164.01.T01:CDS |
| AATTATAAGGACCATGCCAA+TGG | 0.575784 | 2:-11305519 | MsG0280007164.01.T01:CDS |
| ACATATATGCAGACGCGGGC+AGG | 0.472740 | 2:-11305361 | MsG0280007164.01.T01:CDS |
| ACATGCAAGTATGTGCCTAG+AGG | 0.703396 | 2:-11305753 | MsG0280007164.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAGAAGCTGAGGAAATTATA+AGG | - | Chr2:11306802-11306821 | MsG0280007164.01.T01:CDS | 30.0% | |
| AGCACTCCAATAAATGTAAT+TGG | + | Chr2:11306645-11306664 | None:intergenic | 30.0% | |
| ATAAAGATGTGATTTCTTGG+GGG | - | Chr2:11305824-11305843 | MsG0280007164.01.T01:CDS | 30.0% | |
| ATCGCTGGATTCATAAAAAA+TGG | - | Chr2:11306156-11306175 | MsG0280007164.01.T01:CDS | 30.0% | |
| CAAATTGTCATTGAAAGGAA+TGG | + | Chr2:11306427-11306446 | None:intergenic | 30.0% | |
| CAACCATAATGTTTGATATC+TGG | + | Chr2:11306747-11306766 | None:intergenic | 30.0% | |
| CATGGAATGCAATTATATGT+GGG | - | Chr2:11306544-11306563 | MsG0280007164.01.T01:CDS | 30.0% | |
| GAACCAGATATCAAACATTA+TGG | - | Chr2:11306741-11306760 | MsG0280007164.01.T01:CDS | 30.0% | |
| GATAAAGATGTGATTTCTTG+GGG | - | Chr2:11305823-11305842 | MsG0280007164.01.T01:CDS | 30.0% | |
| GTGATAAAGATGTGATTTCT+TGG | - | Chr2:11305821-11305840 | MsG0280007164.01.T01:CDS | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr2 | gene | 11305277 | 11307079 | 11305277 | ID=MsG0280007164.01;Name=MsG0280007164.01 |
| Chr2 | mRNA | 11305277 | 11307079 | 11305277 | ID=MsG0280007164.01.T01;Parent=MsG0280007164.01;Name=MsG0280007164.01.T01;_AED=0.12;_eAED=0.12;_QI=0|-1|0|1|-1|1|1|0|600 |
| Chr2 | exon | 11305277 | 11307079 | 11305277 | ID=MsG0280007164.01.T01:exon:322;Parent=MsG0280007164.01.T01 |
| Chr2 | CDS | 11305277 | 11307079 | 11305277 | ID=MsG0280007164.01.T01:cds;Parent=MsG0280007164.01.T01 |
| Gene Sequence |
>MsG0280007164.01.T01 ATGTACGCAAAATGCGGTGATATTAATAATGCCCAGCTGTTGTTTGATGGTTTTCCTACGTTGGATAGTGTTTCTTGTAATATTATGGTTTCTGGGTATGTGAGAAATGGTCAAATTGATAATGCACGTAAACTGTTTGATGTAATGCCCGGTAAAGGTTGTGTTTCGTATACGACGATGATAATGGGTTTTGTTCAAAATGGATTGTTTCGTGAAGCGATTGAGGTTTTTAAGGATATGAGAAGTTGTGGTGTTGTGCCGAATGATTTGACTTTGGTTAATGTGATATCTGCTTGTTCGCATTTGGGTGAAGTTTTGAATTGTCGAATGGTTCATGGTTTGGTTGTTAAGATGTTTGTTGAGGGGTTAGTTATTGTTTCGACGAATTTGATGCATGCCTATTGTCTTTGTTCGGGTGTGAGGGAAGCAAGGAGGTTGTTTGATGAGATGCCTGAGAGGAATTTGGTTACGTGGAACGTGATGTTGAATGGATATGCGAAGACGGGACTTGTTGACAGGGCTAGGGAGTTGTTTGACGGGATTTGTGATAAAGATGTGATTTCTTGGGGGACGATGATTGACGGTTATATTCAAAAAGGTCGTTTGCGTGAAGCTTTGGAGATTTATCGTGCAATGCTGCAAACTGGACACAGACCTAATGAAGTTATGATAGTGAATTTGGTTTCAGCATGTGGTCGTGGGACTGCAATTGTTGATGGTTTGCAATTGCATGGAACAGTTGTTAAGAGAGGCTTTGATTGTCATAATTTTATACAGACTACAATCATCTATTTTTATGCAGCGTGTGGGATGATGGACCTTGCTTGTTTGCAATTTGAAGTGGGTGTAAAGGATCACTTAGAATCATGGAATGCTCTTATCGCTGGATTCATAAAAAATGGAATGATGGATCAGGCAATGAAAACCTTTGATAAGATGCCTGTAAGAGATGTGTTTTCATGGAGTACCATGATTTCTGGCTATGCGCAAACTGAACAGCCCAAAATGGCTCTCGAACTTTTCCATAAAATGGTAGCCTGGGGAATCAAACCAAATGAAGTTACCATGGTGAGTGTATTCTCTGCAATTGCCACATTAGGTACATTGCAGGATGGAAAATTGGCCCATGAATACATGCGTAGTGAATCCATTCCTTTCAATGACAATTTGCGTGCTGCTCTGATTGATATGTATGCAAAATGTGGGAGCATCAACTCTGCCCTTCAATTTTTCAATCAGATTCGAGATGGAGTCTCTTCTGTGTCACCATGGAATGCAATTATATGTGGGTTAGCCTCGCATGGACATGCAAGTATGTGCCTAGAGGTTTTTTCTGATATGCAGAGATGTCATATTAAACCAAATCCAATTACATTTATTGGAGTGCTAAGTGCTTGCTGTCATGCCGGGTTGGTGGAGCTTGGAAGGAGAATATTTGAAACAATGAAAAGCGCACATAATGTGGAACCAGATATCAAACATTATGGTTGTATGATTGATCTTTTGGGCAGAGCTGGGCTATTAGAAGAAGCTGAGGAAATTATAAGGACCATGCCAATGGAGGCCGATATTGTGATATGGGGAACTTTATTAGCAGCATGTAGAACTCATGGAAATGTTCACATAGGAGAGAGGGCTGCAGAGAATTTGGCGAGGTTGTCGCCGTCTCATGGTGGAGGCAAAGTTCTCCTATCAAACATATATGCAGACGCGGGCAGGTGGGAGGATGTATCATTTGTAAGAAGTGTCATGCAGGGTCAGACAATGGAGCGAGAACCTGGCTGCAGTGGTTTTGTCATGTAG |
| Protein sequence |
>MsG0280007164.01.T01 MYAKCGDINNAQLLFDGFPTLDSVSCNIMVSGYVRNGQIDNARKLFDVMPGKGCVSYTTMIMGFVQNGLFREAIEVFKDMRSCGVVPNDLTLVNVISACSHLGEVLNCRMVHGLVVKMFVEGLVIVSTNLMHAYCLCSGVREARRLFDEMPERNLVTWNVMLNGYAKTGLVDRARELFDGICDKDVISWGTMIDGYIQKGRLREALEIYRAMLQTGHRPNEVMIVNLVSACGRGTAIVDGLQLHGTVVKRGFDCHNFIQTTIIYFYAACGMMDLACLQFEVGVKDHLESWNALIAGFIKNGMMDQAMKTFDKMPVRDVFSWSTMISGYAQTEQPKMALELFHKMVAWGIKPNEVTMVSVFSAIATLGTLQDGKLAHEYMRSESIPFNDNLRAALIDMYAKCGSINSALQFFNQIRDGVSSVSPWNAIICGLASHGHASMCLEVFSDMQRCHIKPNPITFIGVLSACCHAGLVELGRRIFETMKSAHNVEPDIKHYGCMIDLLGRAGLLEEAEEIIRTMPMEADIVIWGTLLAACRTHGNVHIGERAAENLARLSPSHGGGKVLLSNIYADAGRWEDVSFVRSVMQGQTMEREPGCSGFVM* |
