AlfalfaGEDB | MsG0480021363.01

M. sativa cultivar ZhongmuNo.1 / MsG0480021363.01

KEGG: K14488
GO: GO:0009733
PFAM: PF02519

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480021363.01.T01	XP_003607135.1	86.957	92	12	0	1	92	1	92	1.43E-54	174

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480021363.01.T01	P32295	74.725	91	23	0	1	91	1	91	1.50E-46	146

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480021363.01.T01	G7JM89	86.957	92	12	0	1	92	1	92	6.83e-55	174

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0280010975.01	MsG0480021363.01	0.801797	7.598778e-49	3.662025e-46
MsG0480021363.01	MsG0480021370.01	0.806609	7.571016e-50	4.127185e-47
MsG0480021363.01	MsG0480021374.01	0.803432	3.496554e-49	1.756810e-46
MsG0480021363.01	MsG0780039975.01	0.803098	4.100124e-49	2.042362e-46

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PPI

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Gene1	Gene2	Type
MsG0480021386.01	MsG0480021363.01	PPI
MsG0480021386.01	MsG0480021363.01	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480021363.01.T01	MTR_4g072620	86.957	92	12	0	1	92	1	92	1.73e-58	174
MsG0480021363.01.T01	MTR_4g072480	85.870	92	13	0	1	92	1	92	1.70e-57	172
MsG0480021363.01.T01	MTR_4g072730	84.615	91	14	0	1	91	1	91	3.76e-56	168
MsG0480021363.01.T01	MTR_4g072570	85.870	92	13	0	1	92	1	92	1.08e-55	167
MsG0480021363.01.T01	MTR_4g072500	83.696	92	15	0	1	92	1	92	1.26e-55	167
MsG0480021363.01.T01	MTR_4g072680	84.783	92	14	0	1	92	1	92	1.87e-55	166
MsG0480021363.01.T01	MTR_4g072460	82.609	92	16	0	1	92	1	92	9.09e-54	162
MsG0480021363.01.T01	MTR_4g072530	80.000	90	18	0	1	90	1	90	2.25e-52	159
MsG0480021363.01.T01	MTR_4g072640	80.000	90	18	0	1	90	1	90	2.32e-51	156
MsG0480021363.01.T01	MTR_4g072660	80.435	92	13	1	1	92	1	87	8.69e-51	154

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480021363.01.T01	AT4G34770	60.000	90	34	1	4	91	14	103	8.80e-34	112
MsG0480021363.01.T01	AT3G03840	59.341	91	32	2	3	91	8	95	1.01e-32	109
MsG0480021363.01.T01	AT5G18020	65.432	81	21	2	8	87	13	87	5.70e-32	107
MsG0480021363.01.T01	AT4G38840	55.556	99	35	3	1	90	1	99	1.16e-31	107
MsG0480021363.01.T01	AT2G21200	57.647	85	34	2	7	89	2	86	1.49e-31	106
MsG0480021363.01.T01	AT5G18060	56.044	91	38	2	1	90	1	90	1.68e-31	106
MsG0480021363.01.T01	AT5G18080	55.556	90	38	2	1	89	1	89	4.80e-31	105
MsG0480021363.01.T01	AT4G38850	58.889	90	30	3	1	87	1	86	5.68e-31	105
MsG0480021363.01.T01	AT3G03850	59.341	91	30	2	3	91	8	93	8.94e-31	104
MsG0480021363.01.T01	AT4G38825	59.036	83	33	1	9	90	7	89	1.12e-30	104

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Find 26 sgRNAs with CRISPR-Local

Find 26 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATCTGTGGAAGTTCCAA+AGG	0.548759	4:-57095310	MsG0480021363.01.T01:CDS
AAATCTGTGGAAGTTCCAAA+GGG	0.612114	4:-57095309	MsG0480021363.01.T01:CDS
ACAAATCTTGAAACAATGGT+TGG	0.515696	4:+57095223	MsG0480021364.01.T02:intron
ACAAGCATCATCAAAATCTG+TGG	0.600941	4:-57095322	MsG0480021363.01.T01:CDS
AGATTTGTTGAGTCAATCTG+AGG	0.607255	4:-57095208	MsG0480021363.01.T01:CDS
AGTCAATCTGAGGAAGAGTT+CGG	0.507563	4:-57095198	MsG0480021363.01.T01:CDS
ATATAACTTCTTGCTTGAAT+GGG	0.446395	4:-57095119	MsG0480021363.01.T01:CDS
ATGTTGGAGAGAGACAAAAG+CGG	0.822067	4:-57095272	MsG0480021363.01.T01:CDS
CATATAACTTCTTGCTTGAA+TGG	0.302470	4:-57095120	MsG0480021363.01.T01:CDS
CCGTTTACATACTATTCTAA+AGG	0.397138	4:-57095361	MsG0480021363.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ACAAATCTTGAAACAATGGT+TGG	+	Chr4:57095262-57095281	MsG0480021364.01.T02:intron	30.0%
CATATAACTTCTTGCTTGAA+TGG	-	Chr4:57095362-57095381	MsG0480021363.01.T01:CDS	30.0%
CCGTTTACATACTATTCTAA+AGG	-	Chr4:57095121-57095140	MsG0480021363.01.T01:CDS	30.0%
CTCAACAAATCTTGAAACAA+TGG	+	Chr4:57095266-57095285	MsG0480021364.01.T02:intron	30.0%
TATATGTTGGAAGACATCTT+CGG	+	Chr4:57095348-57095367	MsG0480021364.01.T02:intron	30.0%
TCAAGCAAGAAGTTATATGT+TGG	+	Chr4:57095361-57095380	MsG0480021364.01.T02:intron	30.0%
AAAATCTGTGGAAGTTCCAA+AGG	-	Chr4:57095172-57095191	MsG0480021363.01.T01:CDS	35.0%
AAATCTGTGGAAGTTCCAAA+GGG	-	Chr4:57095173-57095192	MsG0480021363.01.T01:CDS	35.0%
ACAAGCATCATCAAAATCTG+TGG	-	Chr4:57095160-57095179	MsG0480021363.01.T01:CDS	35.0%
TTCGGATATGATCATCCAAT+GGG	-	Chr4:57095302-57095321	MsG0480021363.01.T01:CDS	35.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr4	gene	57095113	57095391	57095113	ID=MsG0480021363.01;Name=MsG0480021363.01
Chr4	mRNA	57095113	57095391	57095113	ID=MsG0480021363.01.T01;Parent=MsG0480021363.01;Name=MsG0480021363.01.T01;_AED=0.47;_eAED=0.47;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|92
Chr4	exon	57095113	57095391	57095113	ID=MsG0480021363.01.T01:exon:16253;Parent=MsG0480021363.01.T01
Chr4	CDS	57095113	57095391	57095113	ID=MsG0480021363.01.T01:cds;Parent=MsG0480021363.01.T01

Gene Sequence	>MsG0480021363.01.T01 ATGGGTTTCCGTTTACATACTATTCTAAAGGGTTCAGTAACTGCTAGACAAGCATCATCAAAATCTGTGGAAGTTCCAAAGGGCTATGTTGCAGTGTATGTTGGAGAGAGACAAAAGCGGTTTGTGATCCCTATCTCATACTTGAACCAACCATTGTTTCAAGATTTGTTGAGTCAATCTGAGGAAGAGTTCGGATATGATCATCCAATGGGTGGCCTCACCATTCCATGCTCCGAAGATGTCTTCCAACATATAACTTCTTGCTTGAATGGGCTATGA
Protein sequence	>MsG0480021363.01.T01 MGFRLHTILKGSVTARQASSKSVEVPKGYVAVYVGERQKRFVIPISYLNQPLFQDLLSQSEEEFGYDHPMGGLTIPCSEDVFQHITSCLNGL*