AlfalfaGEDB | MsG0480021363.01

M. sativa cultivar ZhongmuNo.1 / MsG0480021363.01

KEGG: K14488
GO: GO:0009733
PFAM: PF02519

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480021363.01.T01	XP_003607135.1	86.957	92	12	0	1	92	1	92	1.43E-54	174

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480021363.01.T01	P32295	74.725	91	23	0	1	91	1	91	1.50E-46	146

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480021363.01.T01	G7JM89	86.957	92	12	0	1	92	1	92	6.83e-55	174

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0280010975.01	MsG0480021363.01	0.801797	7.598778e-49	3.662025e-46
MsG0480021363.01	MsG0480021370.01	0.806609	7.571016e-50	4.127185e-47
MsG0480021363.01	MsG0480021374.01	0.803432	3.496554e-49	1.756810e-46
MsG0480021363.01	MsG0780039975.01	0.803098	4.100124e-49	2.042362e-46

PPI

Gene1	Gene2	Type
MsG0480021386.01	MsG0480021363.01	PPI
MsG0480021386.01	MsG0480021363.01	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480021363.01.T01	MTR_4g072620	86.957	92	12	0	1	92	1	92	1.73e-58	174
MsG0480021363.01.T01	MTR_4g072480	85.870	92	13	0	1	92	1	92	1.70e-57	172
MsG0480021363.01.T01	MTR_4g072730	84.615	91	14	0	1	91	1	91	3.76e-56	168
MsG0480021363.01.T01	MTR_4g072570	85.870	92	13	0	1	92	1	92	1.08e-55	167
MsG0480021363.01.T01	MTR_4g072500	83.696	92	15	0	1	92	1	92	1.26e-55	167
MsG0480021363.01.T01	MTR_4g072680	84.783	92	14	0	1	92	1	92	1.87e-55	166
MsG0480021363.01.T01	MTR_4g072460	82.609	92	16	0	1	92	1	92	9.09e-54	162
MsG0480021363.01.T01	MTR_4g072530	80.000	90	18	0	1	90	1	90	2.25e-52	159
MsG0480021363.01.T01	MTR_4g072640	80.000	90	18	0	1	90	1	90	2.32e-51	156
MsG0480021363.01.T01	MTR_4g072660	80.435	92	13	1	1	92	1	87	8.69e-51	154
MsG0480021363.01.T01	MTR_4g072310	76.344	93	21	1	1	92	1	93	2.63e-49	151
MsG0480021363.01.T01	MTR_4g072860	78.495	93	19	1	1	92	1	93	2.90e-49	151
MsG0480021363.01.T01	MTR_4g072270	73.118	93	24	1	1	92	1	93	2.12e-47	146
MsG0480021363.01.T01	MTR_3g084150	73.913	92	24	0	1	92	1	92	2.32e-47	146
MsG0480021363.01.T01	MTR_4g072230	76.344	93	19	2	1	92	1	91	3.26e-47	145
MsG0480021363.01.T01	MTR_3g084180	72.826	92	25	0	1	92	1	92	2.77e-46	143
MsG0480021363.01.T01	MTR_8g461410	71.739	92	26	0	1	92	1	92	5.58e-46	142
MsG0480021363.01.T01	MTR_4g072880	68.817	93	28	1	1	92	1	93	1.14e-45	142
MsG0480021363.01.T01	MTR_4g072250	75.269	93	21	2	1	92	1	92	1.64e-45	141
MsG0480021363.01.T01	MTR_5g008360	81.250	80	15	0	13	92	4	83	1.74e-44	139
MsG0480021363.01.T01	MTR_4g072240	70.330	91	26	1	1	90	1	91	3.43e-44	138
MsG0480021363.01.T01	MTR_4g072870	70.330	91	26	1	1	90	1	91	1.06e-43	137
MsG0480021363.01.T01	MTR_3g084250	70.652	92	27	0	1	92	1	92	1.70e-43	136
MsG0480021363.01.T01	MTR_3g084240	68.478	92	29	0	1	92	1	92	2.33e-43	136
MsG0480021363.01.T01	MTR_3g117630	75.581	86	21	0	7	92	7	92	3.20e-43	135
MsG0480021363.01.T01	MTR_3g084170	68.478	92	29	0	1	92	1	92	6.68e-43	135
MsG0480021363.01.T01	MTR_4g072220	66.667	93	30	1	1	92	1	93	1.93e-42	134
MsG0480021363.01.T01	MTR_3g110042	73.563	87	23	0	1	87	1	87	2.24e-42	134
MsG0480021363.01.T01	MTR_4g072300	67.033	91	29	1	1	90	1	91	2.34e-42	133
MsG0480021363.01.T01	MTR_4g072850	70.213	94	26	1	1	92	1	94	2.34e-42	134
MsG0480021363.01.T01	MTR_4g072490	71.111	90	21	1	1	90	1	85	8.72e-42	132
MsG0480021363.01.T01	MTR_4g072600	72.222	90	20	1	1	90	1	85	1.52e-41	131
MsG0480021363.01.T01	MTR_3g084210	70.652	92	27	0	1	92	1	92	1.59e-41	131
MsG0480021363.01.T01	MTR_5g008370	82.432	74	13	0	18	91	18	91	1.73e-41	133
MsG0480021363.01.T01	MTR_4g072330	70.000	90	25	1	1	90	1	88	4.54e-41	130
MsG0480021363.01.T01	MTR_4g072370	71.111	90	24	1	1	90	1	88	4.59e-41	130
MsG0480021363.01.T01	MTR_4g050700	67.742	93	29	1	1	92	1	93	6.60e-41	130
MsG0480021363.01.T01	MTR_2g012410	67.742	93	29	1	1	92	1	93	6.60e-41	130
MsG0480021363.01.T01	MTR_3g110032	67.742	93	29	1	1	92	1	93	1.00e-40	129
MsG0480021363.01.T01	MTR_4g072470	73.333	90	19	1	1	90	1	85	1.12e-40	129
MsG0480021363.01.T01	MTR_3g084220	65.217	92	32	0	1	92	1	92	1.17e-40	129
MsG0480021363.01.T01	MTR_3g084190	70.652	92	27	0	1	92	1	92	1.26e-40	129
MsG0480021363.01.T01	MTR_4g072740	70.000	90	22	1	1	90	1	85	1.72e-40	129
MsG0480021363.01.T01	MTR_4g072890	64.835	91	31	1	1	90	1	91	3.12e-40	128
MsG0480021363.01.T01	MTR_4g072260	63.441	93	33	1	1	92	1	93	3.82e-40	128
MsG0480021363.01.T01	MTR_4g072580	68.889	90	23	1	1	90	1	85	1.06e-39	127
MsG0480021363.01.T01	MTR_8g461420	70.000	90	27	0	1	90	1	90	1.38e-39	126
MsG0480021363.01.T01	MTR_3g084160	69.565	92	28	0	1	92	1	92	1.63e-39	126
MsG0480021363.01.T01	MTR_3g084230	70.000	90	27	0	1	90	1	90	5.15e-39	125
MsG0480021363.01.T01	MTR_3g110038	64.130	92	33	0	1	92	1	92	6.93e-39	125
MsG0480021363.01.T01	MTR_4g072830	68.539	89	26	1	1	89	1	87	7.29e-39	124
MsG0480021363.01.T01	MTR_4g072940	77.778	72	16	0	21	92	53	124	1.10e-38	125
MsG0480021363.01.T01	MTR_3g084260	64.516	93	32	1	1	92	1	93	1.66e-38	124
MsG0480021363.01.T01	MTR_4g072630	64.444	90	28	1	1	90	1	86	2.19e-38	123
MsG0480021363.01.T01	MTR_4g072550	75.342	73	18	0	20	92	31	103	7.05e-38	122
MsG0480021363.01.T01	MTR_3g110045	69.892	93	26	1	2	92	59	151	1.88e-37	123
MsG0480021363.01.T01	MTR_4g072610	67.778	90	23	1	1	90	1	84	2.01e-37	120
MsG0480021363.01.T01	MTR_4g072590	71.111	90	21	1	1	90	1	85	3.53e-37	120
MsG0480021363.01.T01	MTR_4g072540	67.778	90	25	1	1	90	1	86	3.92e-37	120
MsG0480021363.01.T01	MTR_4g072465	63.333	90	29	1	1	90	1	86	3.92e-37	120
MsG0480021363.01.T01	MTR_4g072380	68.132	91	23	2	1	90	1	86	1.19e-36	119
MsG0480021363.01.T01	MTR_4g072720	66.667	90	26	1	1	90	1	86	3.12e-36	118
MsG0480021363.01.T01	MTR_4g072820	67.033	91	23	2	1	90	1	85	1.61e-35	116
MsG0480021363.01.T01	MTR_4g072670	66.667	90	26	1	1	90	1	86	4.28e-35	115
MsG0480021363.01.T01	MTR_4g072650	63.333	90	27	1	1	90	1	84	1.88e-34	113
MsG0480021363.01.T01	MTR_4g072510	64.444	90	28	1	1	90	1	86	5.16e-34	112
MsG0480021363.01.T01	MTR_4g072450	70.000	90	22	1	1	90	1	85	6.81e-34	112
MsG0480021363.01.T01	MTR_5g008350	67.089	79	21	1	1	79	1	74	1.07e-32	108
MsG0480021363.01.T01	MTR_4g072910	68.000	75	23	1	16	90	27	100	1.77e-32	108
MsG0480021363.01.T01	MTR_4g072980	58.065	93	33	2	1	92	8	95	3.65e-32	108
MsG0480021363.01.T01	MTR_4g072750	63.529	85	27	2	1	81	1	85	5.35e-32	107
MsG0480021363.01.T01	MTR_4g072690	66.667	90	25	1	1	90	1	85	8.57e-32	106
MsG0480021363.01.T01	MTR_4g072770	61.111	90	25	1	1	90	1	80	2.42e-31	105
MsG0480021363.01.T01	MTR_4g072560	57.831	83	34	1	8	89	15	97	3.91e-31	105
MsG0480021363.01.T01	MTR_4g072290	58.065	93	24	2	1	92	1	79	9.40e-31	103
MsG0480021363.01.T01	MTR_3g117640	68.116	69	21	1	24	91	33	101	1.65e-29	101
MsG0480021363.01.T01	MTR_3g084200	61.111	72	28	0	1	72	1	72	1.54e-28	99.4
MsG0480021363.01.T01	MTR_4g072280	60.000	75	29	1	1	74	1	75	4.23e-28	97.4
MsG0480021363.01.T01	MTR_3g110035	63.636	66	24	0	27	92	73	138	2.85e-26	94.4
MsG0480021363.01.T01	MTR_6g077850	56.716	67	29	0	24	90	33	99	1.04e-25	92.0
MsG0480021363.01.T01	MTR_3g113310	58.209	67	27	1	23	89	40	105	2.08e-25	91.3
MsG0480021363.01.T01	MTR_3g117610	59.701	67	26	1	23	89	43	108	1.28e-24	89.4
MsG0480021363.01.T01	MTR_4g072190	51.765	85	39	2	5	89	17	99	3.71e-24	87.8
MsG0480021363.01.T01	MTR_4g072930	51.852	81	34	2	15	90	30	110	1.02e-22	84.7
MsG0480021363.01.T01	MTR_5g021820	54.545	66	29	1	24	89	39	103	2.72e-21	80.9
MsG0480021363.01.T01	MTR_8g076040	48.101	79	40	1	11	89	27	104	3.66e-21	80.5
MsG0480021363.01.T01	MTR_8g461400	45.652	92	24	1	1	92	1	66	1.74e-20	77.8
MsG0480021363.01.T01	MTR_4g124750	51.515	66	31	1	24	89	68	132	2.35e-19	77.4
MsG0480021363.01.T01	MTR_2g043960	51.515	66	31	1	24	89	75	139	3.45e-19	77.0
MsG0480021363.01.T01	MTR_4g072840	49.451	91	14	3	1	90	1	60	3.87e-19	73.9
MsG0480021363.01.T01	MTR_8g461210	43.023	86	48	1	4	89	14	98	5.73e-18	72.8
MsG0480021363.01.T01	MTR_7g118010	48.529	68	32	2	25	90	23	89	4.12e-17	70.1
MsG0480021363.01.T01	MTR_3g092220	41.176	85	44	3	1	83	12	92	8.31e-17	69.7
MsG0480021363.01.T01	MTR_4g005310	48.052	77	34	2	15	85	47	123	1.08e-16	70.5
MsG0480021363.01.T01	MTR_1g024085	44.578	83	42	2	4	83	13	94	1.65e-16	69.7
MsG0480021363.01.T01	MTR_0388s0020	40.000	85	47	2	8	89	58	141	2.23e-16	69.7
MsG0480021363.01.T01	MTR_8g026730	45.588	68	34	2	25	90	27	93	3.99e-16	67.8
MsG0480021363.01.T01	MTR_1g061570	47.059	68	35	1	24	91	18	84	1.19e-15	67.0
MsG0480021363.01.T01	MTR_1g009150	50.000	68	33	1	18	85	64	130	2.21e-15	67.8
MsG0480021363.01.T01	MTR_1g059770	45.714	70	34	2	25	91	49	117	2.55e-15	66.2
MsG0480021363.01.T01	MTR_4g072160	47.887	71	34	2	16	85	28	96	2.89e-15	66.2
MsG0480021363.01.T01	MTR_4g025830	43.939	66	33	2	25	87	79	143	2.97e-15	67.0
MsG0480021363.01.T01	MTR_1g063950	43.056	72	40	1	17	88	40	110	5.50e-15	65.5
MsG0480021363.01.T01	MTR_1g094750	50.000	62	30	1	24	85	49	109	7.05e-15	64.7
MsG0480021363.01.T01	MTR_3g109160	43.750	80	44	1	12	91	56	134	1.56e-14	65.1
MsG0480021363.01.T01	MTR_7g101740	48.438	64	32	1	24	87	39	101	3.81e-14	62.8
MsG0480021363.01.T01	MTR_4g005320	49.180	61	30	1	25	85	70	129	6.09e-14	63.5
MsG0480021363.01.T01	MTR_2g066830	39.744	78	46	1	13	90	46	122	2.11e-13	61.6
MsG0480021363.01.T01	MTR_3g097120	44.595	74	32	2	20	85	4	76	9.47e-13	59.3
MsG0480021363.01.T01	MTR_8g101520	39.024	82	49	1	9	90	34	114	1.17e-12	60.1
MsG0480021363.01.T01	MTR_7g104940	37.333	75	42	2	15	89	44	113	1.40e-12	59.3
MsG0480021363.01.T01	MTR_7g405740	45.902	61	32	1	25	85	67	126	2.17e-12	59.3
MsG0480021363.01.T01	MTR_8g096470	46.667	60	31	1	26	85	47	105	2.39e-12	58.2
MsG0480021363.01.T01	MTR_7g104950	35.000	80	46	2	10	89	40	113	2.66e-12	58.5
MsG0480021363.01.T01	MTR_3g105900	72.222	36	10	0	24	59	1171	1206	2.73e-12	61.2
MsG0480021363.01.T01	MTR_3g098970	32.051	78	52	1	15	92	43	119	6.87e-12	57.8
MsG0480021363.01.T01	MTR_8g101530	39.130	69	41	1	19	87	34	101	1.90e-11	56.6
MsG0480021363.01.T01	MTR_8g096440	39.130	69	41	1	14	82	34	101	3.77e-11	55.8
MsG0480021363.01.T01	MTR_2g095430	39.506	81	41	3	5	78	22	101	4.87e-11	55.5
MsG0480021363.01.T01	MTR_8g096500	46.552	58	30	1	25	82	49	105	5.93e-11	55.5
MsG0480021363.01.T01	MTR_8g094980	40.789	76	31	2	23	85	1	75	9.99e-11	53.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480021363.01.T01	AT4G34770	60.000	90	34	1	4	91	14	103	8.80e-34	112
MsG0480021363.01.T01	AT3G03840	59.341	91	32	2	3	91	8	95	1.01e-32	109
MsG0480021363.01.T01	AT5G18020	65.432	81	21	2	8	87	13	87	5.70e-32	107
MsG0480021363.01.T01	AT4G38840	55.556	99	35	3	1	90	1	99	1.16e-31	107
MsG0480021363.01.T01	AT2G21200	57.647	85	34	2	7	89	2	86	1.49e-31	106
MsG0480021363.01.T01	AT5G18060	56.044	91	38	2	1	90	1	90	1.68e-31	106
MsG0480021363.01.T01	AT5G18080	55.556	90	38	2	1	89	1	89	4.80e-31	105
MsG0480021363.01.T01	AT4G38850	58.889	90	30	3	1	87	1	86	5.68e-31	105
MsG0480021363.01.T01	AT3G03850	59.341	91	30	2	3	91	8	93	8.94e-31	104
MsG0480021363.01.T01	AT4G38825	59.036	83	33	1	9	90	7	89	1.12e-30	104
MsG0480021363.01.T01	AT3G03820	57.471	87	31	2	8	91	13	96	1.66e-30	103
MsG0480021363.01.T01	AT5G18050	69.697	66	19	1	25	89	24	89	1.89e-30	103
MsG0480021363.01.T01	AT5G18030	65.278	72	24	1	20	90	17	88	2.19e-30	103
MsG0480021363.01.T01	AT2G21210	49.474	95	43	2	1	90	1	95	8.81e-30	102
MsG0480021363.01.T01	AT5G18010	52.747	91	41	2	1	90	1	90	2.37e-29	100
MsG0480021363.01.T01	AT2G21210	49.474	95	43	2	1	90	68	162	4.60e-29	102
MsG0480021363.01.T01	AT4G34800	55.294	85	31	2	1	82	1	81	7.26e-29	99.8
MsG0480021363.01.T01	AT4G34810	67.742	62	16	1	25	82	48	109	3.44e-27	96.3
MsG0480021363.01.T01	AT3G03830	53.409	88	32	2	3	87	8	89	8.00e-26	92.0
MsG0480021363.01.T01	AT4G13790	44.944	89	47	1	1	87	1	89	1.43e-25	91.3
MsG0480021363.01.T01	AT4G34790	61.538	65	23	1	25	87	39	103	1.46e-24	89.4
MsG0480021363.01.T01	AT4G34780	61.429	70	24	1	23	89	26	95	3.57e-24	88.2
MsG0480021363.01.T01	AT1G75580	52.500	80	37	1	10	89	29	107	1.10e-23	87.0
MsG0480021363.01.T01	AT4G38860	55.882	68	30	0	23	90	38	105	2.08e-23	86.3
MsG0480021363.01.T01	AT4G34760	57.353	68	28	1	23	90	41	107	2.35e-23	86.3
MsG0480021363.01.T01	AT2G21220	56.716	67	28	1	23	89	38	103	3.13e-23	85.9
MsG0480021363.01.T01	AT1G19830	56.164	73	30	2	18	89	41	112	3.66e-20	78.6
MsG0480021363.01.T01	AT3G43120	41.573	89	51	1	3	91	57	144	3.82e-20	79.3
MsG0480021363.01.T01	AT2G16580	55.385	65	28	1	23	87	42	105	5.56e-20	77.8
MsG0480021363.01.T01	AT4G36110	54.688	64	28	1	24	87	39	101	2.14e-19	76.3
MsG0480021363.01.T01	AT5G66260	48.315	89	42	2	5	90	11	98	2.54e-19	75.9
MsG0480021363.01.T01	AT5G20810	51.515	66	31	1	24	89	78	142	7.06e-19	76.3
MsG0480021363.01.T01	AT5G20810	47.945	73	37	1	17	89	71	142	1.24e-18	76.3
MsG0480021363.01.T01	AT2G18010	53.125	64	29	1	24	87	47	109	2.27e-18	73.6
MsG0480021363.01.T01	AT3G20220	50.000	76	32	3	11	85	37	107	7.24e-18	72.4
MsG0480021363.01.T01	AT2G46690	40.698	86	47	2	9	91	8	92	1.12e-17	72.0
MsG0480021363.01.T01	AT1G75590	50.685	73	31	2	15	83	28	99	1.44e-16	70.1
MsG0480021363.01.T01	AT5G10990	51.667	60	28	1	24	83	40	98	3.33e-16	68.9
MsG0480021363.01.T01	AT4G34750	45.312	64	34	1	24	87	36	98	4.75e-16	68.6
MsG0480021363.01.T01	AT4G34750	45.312	64	34	1	24	87	36	98	4.75e-16	68.6
MsG0480021363.01.T01	AT3G61900	44.286	70	35	2	24	90	29	97	7.43e-16	67.8
MsG0480021363.01.T01	AT3G51200	53.125	64	28	2	20	82	34	96	8.89e-16	67.0
MsG0480021363.01.T01	AT3G12830	41.379	87	49	2	4	90	30	114	1.07e-15	67.4
MsG0480021363.01.T01	AT1G19840	45.238	84	41	2	4	83	16	98	1.25e-15	67.8
MsG0480021363.01.T01	AT2G37030	47.692	65	33	1	24	88	48	111	2.98e-15	65.9
MsG0480021363.01.T01	AT4G00880	47.826	69	33	2	24	90	27	94	4.30e-15	65.5
MsG0480021363.01.T01	AT5G53590	43.056	72	35	2	24	90	45	115	9.58e-15	65.1
MsG0480021363.01.T01	AT3G60690	45.455	66	32	2	25	87	88	152	2.64e-14	64.7
MsG0480021363.01.T01	AT2G45210	40.845	71	38	2	20	87	74	143	4.41e-14	63.9
MsG0480021363.01.T01	AT2G36210	41.333	75	44	0	15	89	44	118	1.12e-13	62.4
MsG0480021363.01.T01	AT3G03847	49.275	69	30	2	18	82	60	127	1.41e-13	62.0
MsG0480021363.01.T01	AT1G56150	46.970	66	34	1	25	90	38	102	1.50e-13	61.2
MsG0480021363.01.T01	AT4G31320	45.455	66	35	1	20	85	75	139	1.65e-13	62.8
MsG0480021363.01.T01	AT2G24400	50.820	61	29	1	25	85	64	123	4.46e-13	61.6
MsG0480021363.01.T01	AT1G16510	44.615	65	35	1	25	89	54	117	1.71e-12	59.3
MsG0480021363.01.T01	AT3G53250	40.000	65	35	1	24	88	51	111	1.86e-12	58.9
MsG0480021363.01.T01	AT4G12410	43.750	64	32	2	25	85	76	138	1.95e-12	59.7
MsG0480021363.01.T01	AT3G09870	48.571	70	33	2	18	85	38	106	2.20e-12	58.5
MsG0480021363.01.T01	AT3G53250	40.000	65	35	1	24	88	82	142	3.18e-12	58.9
MsG0480021363.01.T01	AT1G79130	44.615	65	35	1	25	89	48	111	5.95e-12	57.8
MsG0480021363.01.T01	AT4G22620	39.706	68	37	2	21	85	75	141	6.64e-12	58.2
MsG0480021363.01.T01	AT4G09530	38.158	76	44	2	19	91	22	97	8.99e-12	56.6
MsG0480021363.01.T01	AT5G03310	45.070	71	32	3	24	88	38	107	1.36e-11	56.2
MsG0480021363.01.T01	AT1G76190	47.458	59	29	2	27	85	23	79	2.80e-11	55.8

Find 26 sgRNAs with CRISPR-Local

Find 26 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CATATAACTTCTTGCTTGAA+TGG	0.302470	4:-57095120	MsG0480021363.01.T01:CDS
GGTTGGTTCAAGTATGAGAT+AGG	0.370602	4:+57095240	MsG0480021364.01.T02:intron
CGTTTACATACTATTCTAAA+GGG	0.377068	4:-57095360	MsG0480021363.01.T01:CDS
CCGTTTACATACTATTCTAA+AGG	0.397138	4:-57095361	MsG0480021363.01.T01:CDS
ATATAACTTCTTGCTTGAAT+GGG	0.446395	4:-57095119	MsG0480021363.01.T01:CDS
CCTTTAGAATAGTATGTAAA+CGG	0.479197	4:+57095361	MsG0480021364.01.T02:intron
TACACTGCAACATAGCCCTT+TGG	0.504635	4:+57095294	MsG0480021364.01.T02:intron
AGTCAATCTGAGGAAGAGTT+CGG	0.507563	4:-57095198	MsG0480021363.01.T01:CDS
ACAAATCTTGAAACAATGGT+TGG	0.515696	4:+57095223	MsG0480021364.01.T02:intron
TCAAGCAAGAAGTTATATGT+TGG	0.522465	4:+57095124	MsG0480021364.01.T02:intron
CTCAACAAATCTTGAAACAA+TGG	0.526990	4:+57095219	MsG0480021364.01.T02:intron
TTCGGATATGATCATCCAAT+GGG	0.535679	4:-57095180	MsG0480021363.01.T01:CDS
AAAATCTGTGGAAGTTCCAA+AGG	0.548759	4:-57095310	MsG0480021363.01.T01:CDS
GGCTATGTTGCAGTGTATGT+TGG	0.553556	4:-57095288	MsG0480021363.01.T01:CDS
TGGAAGACATCTTCGGAGCA+TGG	0.559524	4:+57095144	MsG0480021364.01.T02:intron
GACATCTTCGGAGCATGGAA+TGG	0.559799	4:+57095149	MsG0480021364.01.T02:intron
GTTCGGATATGATCATCCAA+TGG	0.566860	4:-57095181	MsG0480021363.01.T01:CDS
TATATGTTGGAAGACATCTT+CGG	0.572640	4:+57095137	MsG0480021364.01.T02:intron
GTTGGTTCAAGTATGAGATA+GGG	0.585637	4:+57095241	MsG0480021364.01.T02:intron
GGAATGGTGAGGCCACCCAT+TGG	0.588290	4:+57095165	MsG0480021364.01.T02:intron
ACAAGCATCATCAAAATCTG+TGG	0.600941	4:-57095322	MsG0480021363.01.T01:CDS
AGATTTGTTGAGTCAATCTG+AGG	0.607255	4:-57095208	MsG0480021363.01.T01:CDS
AAATCTGTGGAAGTTCCAAA+GGG	0.612114	4:-57095309	MsG0480021363.01.T01:CDS
GGATATGATCATCCAATGGG+TGG	0.661502	4:-57095177	MsG0480021363.01.T01:CDS
CTTCGGAGCATGGAATGGTG+AGG	0.671810	4:+57095154	MsG0480021364.01.T02:intron
ATGTTGGAGAGAGACAAAAG+CGG	0.822067	4:-57095272	MsG0480021363.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	ATATAACTTCTTGCTTGAAT+GGG	-	Chr4:57095363-57095382	MsG0480021363.01.T01:CDS	25.0%
!	CGTTTACATACTATTCTAAA+GGG	-	Chr4:57095122-57095141	MsG0480021363.01.T01:CDS	25.0%
!!!	CCTTTAGAATAGTATGTAAA+CGG	+	Chr4:57095124-57095143	MsG0480021364.01.T02:intron	25.0%
	ACAAATCTTGAAACAATGGT+TGG	+	Chr4:57095262-57095281	MsG0480021364.01.T02:intron	30.0%
	CATATAACTTCTTGCTTGAA+TGG	-	Chr4:57095362-57095381	MsG0480021363.01.T01:CDS	30.0%
	CCGTTTACATACTATTCTAA+AGG	-	Chr4:57095121-57095140	MsG0480021363.01.T01:CDS	30.0%
	CTCAACAAATCTTGAAACAA+TGG	+	Chr4:57095266-57095285	MsG0480021364.01.T02:intron	30.0%
	TATATGTTGGAAGACATCTT+CGG	+	Chr4:57095348-57095367	MsG0480021364.01.T02:intron	30.0%
	TCAAGCAAGAAGTTATATGT+TGG	+	Chr4:57095361-57095380	MsG0480021364.01.T02:intron	30.0%
	AAAATCTGTGGAAGTTCCAA+AGG	-	Chr4:57095172-57095191	MsG0480021363.01.T01:CDS	35.0%
	AAATCTGTGGAAGTTCCAAA+GGG	-	Chr4:57095173-57095192	MsG0480021363.01.T01:CDS	35.0%
	ACAAGCATCATCAAAATCTG+TGG	-	Chr4:57095160-57095179	MsG0480021363.01.T01:CDS	35.0%
	TTCGGATATGATCATCCAAT+GGG	-	Chr4:57095302-57095321	MsG0480021363.01.T01:CDS	35.0%
!	GTTGGTTCAAGTATGAGATA+GGG	+	Chr4:57095244-57095263	MsG0480021364.01.T02:intron	35.0%
!!	AGATTTGTTGAGTCAATCTG+AGG	-	Chr4:57095274-57095293	MsG0480021363.01.T01:CDS	35.0%
	AGTCAATCTGAGGAAGAGTT+CGG	-	Chr4:57095284-57095303	MsG0480021363.01.T01:CDS	40.0%
	ATGTTGGAGAGAGACAAAAG+CGG	-	Chr4:57095210-57095229	MsG0480021363.01.T01:CDS	40.0%
	GTTCGGATATGATCATCCAA+TGG	-	Chr4:57095301-57095320	MsG0480021363.01.T01:CDS	40.0%
!	GGTTGGTTCAAGTATGAGAT+AGG	+	Chr4:57095245-57095264	MsG0480021364.01.T02:intron	40.0%
	GGATATGATCATCCAATGGG+TGG	-	Chr4:57095305-57095324	MsG0480021363.01.T01:CDS	45.0%
	GGCTATGTTGCAGTGTATGT+TGG	-	Chr4:57095194-57095213	MsG0480021363.01.T01:CDS	45.0%
	TACACTGCAACATAGCCCTT+TGG	+	Chr4:57095191-57095210	MsG0480021364.01.T02:intron	45.0%
	GACATCTTCGGAGCATGGAA+TGG	+	Chr4:57095336-57095355	MsG0480021364.01.T02:intron	50.0%
	TGGAAGACATCTTCGGAGCA+TGG	+	Chr4:57095341-57095360	MsG0480021364.01.T02:intron	50.0%
	CTTCGGAGCATGGAATGGTG+AGG	+	Chr4:57095331-57095350	MsG0480021364.01.T02:intron	55.0%
	GGAATGGTGAGGCCACCCAT+TGG	+	Chr4:57095320-57095339	MsG0480021364.01.T02:intron	60.0%

Chromosome	Type	Strat	End	Strand	Name
Chr4	gene	57095113	57095391	57095113	ID=MsG0480021363.01;Name=MsG0480021363.01
Chr4	mRNA	57095113	57095391	57095113	ID=MsG0480021363.01.T01;Parent=MsG0480021363.01;Name=MsG0480021363.01.T01;_AED=0.47;_eAED=0.47;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|92
Chr4	exon	57095113	57095391	57095113	ID=MsG0480021363.01.T01:exon:16253;Parent=MsG0480021363.01.T01
Chr4	CDS	57095113	57095391	57095113	ID=MsG0480021363.01.T01:cds;Parent=MsG0480021363.01.T01

Gene Sequence	>MsG0480021363.01.T01 ATGGGTTTCCGTTTACATACTATTCTAAAGGGTTCAGTAACTGCTAGACAAGCATCATCAAAATCTGTGGAAGTTCCAAAGGGCTATGTTGCAGTGTATGTTGGAGAGAGACAAAAGCGGTTTGTGATCCCTATCTCATACTTGAACCAACCATTGTTTCAAGATTTGTTGAGTCAATCTGAGGAAGAGTTCGGATATGATCATCCAATGGGTGGCCTCACCATTCCATGCTCCGAAGATGTCTTCCAACATATAACTTCTTGCTTGAATGGGCTATGA
Protein sequence	>MsG0480021363.01.T01 MGFRLHTILKGSVTARQASSKSVEVPKGYVAVYVGERQKRFVIPISYLNQPLFQDLLSQSEEEFGYDHPMGGLTIPCSEDVFQHITSCLNGL*