AlfalfaGEDB | MsG0480021384.01

M. sativa cultivar ZhongmuNo.1 / MsG0480021384.01

KEGG: K14488
GO: GO:0009733
PFAM: PF02519

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480021384.01.T01	XP_003607159.2	96.739	92	3	0	1	92	1	92	4.79E-59	186

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480021384.01.T01	P33083	76.923	91	20	1	1	91	1	90	2.81E-46	146

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480021384.01.T01	A0A396I775	96.739	92	3	0	1	92	1	92	2.29e-59	186

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180001270.01	MsG0480021384.01	0.818731	1.681846e-52	1.269126e-49
MsG0480021384.01	MsG0680033377.01	0.806526	7.883476e-50	4.288463e-47
MsG0480021384.01	MsG0680034064.01	0.803688	3.093254e-49	1.564447e-46
MsG0480021384.01	MsG0780039675.01	0.821160	4.680626e-53	3.778666e-50
MsG0380016135.01	MsG0480021384.01	0.806821	6.829896e-50	3.743689e-47
MsG0280010353.01	MsG0480021384.01	0.812273	4.606839e-51	2.916241e-48

PPI

Gene1	Gene2	Type
MsG0480021386.01	MsG0480021384.01	PPI
MsG0480021386.01	MsG0480021384.01	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480021384.01.T01	MTR_4g072880	95.652	92	4	0	1	92	1	92	1.99e-61	182
MsG0480021384.01.T01	MTR_4g072310	88.043	92	11	0	1	92	1	92	1.72e-58	174
MsG0480021384.01.T01	MTR_4g072270	89.130	92	10	0	1	92	1	92	4.48e-58	173
MsG0480021384.01.T01	MTR_4g072230	82.609	92	14	1	1	92	1	90	2.08e-52	159
MsG0480021384.01.T01	MTR_4g072860	79.348	92	19	0	1	92	1	92	4.60e-52	158
MsG0480021384.01.T01	MTR_4g072250	79.348	92	18	1	1	92	1	91	3.72e-50	153
MsG0480021384.01.T01	MTR_3g084150	73.913	92	23	1	1	92	1	91	5.93e-47	145
MsG0480021384.01.T01	MTR_3g084180	72.826	92	24	1	1	92	1	91	3.71e-46	143
MsG0480021384.01.T01	MTR_4g072240	71.429	91	26	0	1	91	1	91	1.13e-45	142
MsG0480021384.01.T01	MTR_4g072640	72.527	91	24	1	1	91	1	90	1.19e-45	142
MsG0480021384.01.T01	MTR_3g110038	72.527	91	24	1	1	91	1	90	1.32e-45	142
MsG0480021384.01.T01	MTR_4g072500	69.565	92	27	1	1	92	1	91	1.56e-45	141
MsG0480021384.01.T01	MTR_3g084170	72.826	92	24	1	1	92	1	91	1.74e-45	141
MsG0480021384.01.T01	MTR_8g461410	70.652	92	26	1	1	92	1	91	2.56e-45	141
MsG0480021384.01.T01	MTR_4g072370	72.527	91	22	1	1	91	1	88	1.17e-44	139
MsG0480021384.01.T01	MTR_3g084240	71.739	92	25	1	1	92	1	91	1.67e-44	139
MsG0480021384.01.T01	MTR_4g072870	69.231	91	28	0	1	91	1	91	2.57e-44	138
MsG0480021384.01.T01	MTR_4g072530	68.132	91	28	1	1	91	1	90	6.20e-44	137
MsG0480021384.01.T01	MTR_3g117630	72.727	88	24	0	4	91	3	90	1.16e-43	137
MsG0480021384.01.T01	MTR_4g072480	69.565	92	27	1	1	92	1	91	1.24e-43	137
MsG0480021384.01.T01	MTR_3g110032	68.478	92	29	0	1	92	1	92	1.37e-43	137
MsG0480021384.01.T01	MTR_4g072570	68.478	92	28	1	1	92	1	91	2.65e-43	136
MsG0480021384.01.T01	MTR_4g050700	67.391	92	30	0	1	92	1	92	2.67e-43	136
MsG0480021384.01.T01	MTR_2g012410	67.391	92	30	0	1	92	1	92	2.67e-43	136
MsG0480021384.01.T01	MTR_4g072300	68.132	91	29	0	1	91	1	91	3.42e-43	135
MsG0480021384.01.T01	MTR_4g072620	68.478	92	28	1	1	92	1	91	3.72e-43	135
MsG0480021384.01.T01	MTR_4g072220	67.033	91	30	0	1	91	1	91	3.79e-43	135
MsG0480021384.01.T01	MTR_4g072730	68.478	92	28	1	1	92	1	91	4.16e-43	135
MsG0480021384.01.T01	MTR_4g072680	69.565	92	27	1	1	92	1	91	4.34e-43	135
MsG0480021384.01.T01	MTR_4g072460	69.565	92	27	1	1	92	1	91	4.39e-43	135
MsG0480021384.01.T01	MTR_4g072850	76.087	92	21	1	1	91	1	92	4.98e-43	135
MsG0480021384.01.T01	MTR_3g084220	69.565	92	27	1	1	92	1	91	5.47e-43	135
MsG0480021384.01.T01	MTR_4g072580	71.429	91	20	1	1	91	1	85	8.25e-43	134
MsG0480021384.01.T01	MTR_4g072890	68.132	91	29	0	1	91	1	91	2.55e-42	133
MsG0480021384.01.T01	MTR_4g072740	71.429	91	20	1	1	91	1	85	4.91e-42	132
MsG0480021384.01.T01	MTR_3g084260	66.304	92	31	0	1	92	1	92	8.84e-42	132
MsG0480021384.01.T01	MTR_4g072260	67.033	91	30	0	1	91	1	91	9.75e-42	132
MsG0480021384.01.T01	MTR_3g110042	71.591	88	24	1	1	88	1	87	1.30e-41	132
MsG0480021384.01.T01	MTR_3g084250	66.304	92	30	1	1	92	1	91	2.23e-41	131
MsG0480021384.01.T01	MTR_4g072490	70.330	91	21	1	1	91	1	85	2.46e-41	130
MsG0480021384.01.T01	MTR_4g072830	70.000	90	24	1	1	90	1	87	4.53e-41	130
MsG0480021384.01.T01	MTR_4g072330	64.835	91	29	1	1	91	1	88	6.29e-41	130
MsG0480021384.01.T01	MTR_4g072470	69.231	91	22	1	1	91	1	85	2.07e-40	128
MsG0480021384.01.T01	MTR_4g072610	69.231	91	21	1	1	91	1	84	2.09e-39	126
MsG0480021384.01.T01	MTR_4g072290	66.304	92	17	1	1	92	1	78	2.37e-39	125
MsG0480021384.01.T01	MTR_5g008360	71.795	78	22	0	15	92	5	82	3.50e-39	125
MsG0480021384.01.T01	MTR_4g072940	72.222	90	24	1	3	91	33	122	4.03e-39	126
MsG0480021384.01.T01	MTR_4g072660	65.217	92	26	1	1	92	1	86	5.70e-39	125
MsG0480021384.01.T01	MTR_4g072465	65.934	91	26	1	1	91	1	86	2.49e-38	123
MsG0480021384.01.T01	MTR_4g072600	65.934	91	25	1	1	91	1	85	2.77e-38	123
MsG0480021384.01.T01	MTR_4g072630	64.835	91	27	1	1	91	1	86	4.55e-38	122
MsG0480021384.01.T01	MTR_5g008370	67.368	95	24	2	1	92	1	91	8.20e-38	124
MsG0480021384.01.T01	MTR_4g072820	66.304	92	23	2	1	91	1	85	2.26e-37	120
MsG0480021384.01.T01	MTR_3g084190	70.330	91	26	1	1	91	1	90	2.44e-37	121
MsG0480021384.01.T01	MTR_4g072540	67.033	91	25	1	1	91	1	86	3.10e-37	120
MsG0480021384.01.T01	MTR_4g072650	67.033	91	23	2	1	91	1	84	3.66e-37	120
MsG0480021384.01.T01	MTR_8g461420	68.132	91	28	1	1	91	1	90	1.06e-36	119
MsG0480021384.01.T01	MTR_3g084160	68.132	91	28	1	1	91	1	90	1.12e-36	119
MsG0480021384.01.T01	MTR_4g072380	67.391	92	23	2	1	91	1	86	2.65e-36	118
MsG0480021384.01.T01	MTR_3g084230	68.132	91	28	1	1	91	1	90	2.69e-36	118
MsG0480021384.01.T01	MTR_3g084210	65.217	92	31	1	1	92	1	91	4.21e-36	117
MsG0480021384.01.T01	MTR_4g072670	64.835	91	27	1	1	91	1	86	8.70e-36	117
MsG0480021384.01.T01	MTR_4g072720	77.143	70	16	0	22	91	17	86	1.39e-35	116
MsG0480021384.01.T01	MTR_4g072550	72.222	72	20	0	21	92	31	102	4.00e-35	115
MsG0480021384.01.T01	MTR_4g072590	68.132	91	23	1	1	91	1	85	2.95e-34	113
MsG0480021384.01.T01	MTR_4g072510	63.736	91	28	1	1	91	1	86	3.82e-34	112
MsG0480021384.01.T01	MTR_3g110045	66.292	89	29	1	3	91	62	149	6.38e-34	114
MsG0480021384.01.T01	MTR_3g084200	69.863	73	21	1	1	73	1	72	2.61e-33	111
MsG0480021384.01.T01	MTR_5g008350	66.250	80	21	1	1	80	1	74	1.14e-32	108
MsG0480021384.01.T01	MTR_4g072910	57.447	94	34	1	4	91	7	100	1.98e-32	108
MsG0480021384.01.T01	MTR_4g072560	52.577	97	39	3	1	90	1	97	2.96e-31	105
MsG0480021384.01.T01	MTR_4g072690	61.538	91	29	1	1	91	1	85	7.23e-31	104
MsG0480021384.01.T01	MTR_4g072980	53.191	94	42	2	1	92	1	94	1.12e-30	104
MsG0480021384.01.T01	MTR_4g072450	61.538	91	29	1	1	91	1	85	4.22e-30	102
MsG0480021384.01.T01	MTR_4g072770	68.182	66	21	0	26	91	15	80	1.45e-28	98.6
MsG0480021384.01.T01	MTR_4g072750	62.667	75	25	2	1	73	1	74	4.44e-28	97.4
MsG0480021384.01.T01	MTR_3g117640	47.525	101	44	2	1	92	1	101	8.44e-28	97.4
MsG0480021384.01.T01	MTR_3g113310	48.315	89	39	2	8	90	18	105	1.19e-26	94.4
MsG0480021384.01.T01	MTR_3g117610	50.549	91	36	2	8	90	19	108	3.66e-26	93.6
MsG0480021384.01.T01	MTR_4g072190	54.321	81	34	2	8	88	20	97	4.36e-26	92.8
MsG0480021384.01.T01	MTR_4g072280	56.000	75	33	0	1	75	1	75	4.63e-26	92.4
MsG0480021384.01.T01	MTR_3g110035	57.895	76	31	1	17	92	63	137	1.23e-25	92.8
MsG0480021384.01.T01	MTR_6g077850	44.318	88	48	1	4	91	13	99	1.85e-25	91.3
MsG0480021384.01.T01	MTR_5g021820	50.575	87	38	2	8	90	18	103	3.49e-25	90.9
MsG0480021384.01.T01	MTR_8g076040	50.588	85	37	2	8	88	19	102	2.15e-24	89.0
MsG0480021384.01.T01	MTR_8g461400	52.174	92	17	2	1	92	1	65	3.23e-24	87.0
MsG0480021384.01.T01	MTR_4g072930	47.059	85	41	1	11	91	26	110	9.13e-23	84.7
MsG0480021384.01.T01	MTR_4g072840	48.913	92	14	3	1	91	1	60	2.43e-19	74.7
MsG0480021384.01.T01	MTR_4g005310	52.857	70	29	2	16	84	55	121	1.53e-17	72.8
MsG0480021384.01.T01	MTR_4g124750	53.030	66	30	1	25	90	68	132	4.09e-17	71.6
MsG0480021384.01.T01	MTR_2g043960	50.725	69	33	1	25	93	75	142	1.07e-16	70.9
MsG0480021384.01.T01	MTR_1g024085	50.000	70	31	2	15	84	29	94	1.17e-16	70.1
MsG0480021384.01.T01	MTR_3g092220	42.500	80	41	3	9	86	18	94	2.33e-15	65.9
MsG0480021384.01.T01	MTR_1g009150	47.887	71	36	1	16	86	61	130	4.98e-15	66.6
MsG0480021384.01.T01	MTR_4g025830	45.588	68	33	2	26	90	79	145	9.27e-15	65.9
MsG0480021384.01.T01	MTR_7g118010	47.059	68	33	2	26	91	23	89	1.02e-14	64.3
MsG0480021384.01.T01	MTR_0388s0020	43.056	72	37	2	20	88	69	139	1.10e-14	65.1
MsG0480021384.01.T01	MTR_1g061570	41.176	85	46	2	8	92	4	84	1.61e-14	64.3
MsG0480021384.01.T01	MTR_1g063950	44.928	69	37	1	15	83	37	104	3.18e-14	63.5
MsG0480021384.01.T01	MTR_4g005320	49.180	61	30	1	26	86	70	129	6.24e-14	63.5
MsG0480021384.01.T01	MTR_3g109160	47.059	68	35	1	26	93	69	135	6.65e-14	63.5
MsG0480021384.01.T01	MTR_1g094750	42.857	77	39	2	11	83	31	106	6.72e-14	62.4
MsG0480021384.01.T01	MTR_1g059770	41.538	65	37	1	26	90	49	112	2.79e-13	61.2
MsG0480021384.01.T01	MTR_4g072160	47.059	68	35	1	23	90	34	100	3.44e-13	61.2
MsG0480021384.01.T01	MTR_8g026730	47.541	61	29	2	26	84	27	86	3.79e-13	60.1
MsG0480021384.01.T01	MTR_7g101740	45.312	64	34	1	25	88	39	101	1.38e-12	58.9
MsG0480021384.01.T01	MTR_8g461210	38.462	91	52	3	4	92	14	102	1.92e-12	58.5
MsG0480021384.01.T01	MTR_3g105900	72.222	36	10	0	25	60	1171	1206	2.16e-12	61.6
MsG0480021384.01.T01	MTR_3g098970	35.000	80	50	2	13	92	41	118	5.28e-12	58.2
MsG0480021384.01.T01	MTR_2g095430	45.588	68	30	3	17	78	34	100	6.64e-12	57.8
MsG0480021384.01.T01	MTR_7g104940	40.323	62	36	1	29	90	53	113	2.05e-11	56.2
MsG0480021384.01.T01	MTR_8g101520	35.632	87	51	2	9	91	29	114	2.56e-11	56.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480021384.01.T01	AT4G38840	63.529	85	30	1	8	91	15	99	5.75e-33	110
MsG0480021384.01.T01	AT5G18020	67.568	74	23	1	17	89	15	88	4.61e-32	107
MsG0480021384.01.T01	AT3G03840	62.791	86	27	2	9	92	13	95	5.96e-32	107
MsG0480021384.01.T01	AT5G18060	65.789	76	25	1	17	91	15	90	6.29e-32	107
MsG0480021384.01.T01	AT5G18030	68.919	74	22	1	19	91	15	88	1.07e-31	106
MsG0480021384.01.T01	AT5G18080	65.789	76	25	1	17	91	15	90	1.61e-31	106
MsG0480021384.01.T01	AT5G18050	64.000	75	26	1	17	90	15	89	5.88e-31	105
MsG0480021384.01.T01	AT5G18010	64.474	76	26	1	17	91	15	90	5.94e-31	105
MsG0480021384.01.T01	AT2G21200	67.606	71	22	1	21	90	16	86	1.55e-30	103
MsG0480021384.01.T01	AT4G38825	65.278	72	24	1	21	91	18	89	9.65e-30	102
MsG0480021384.01.T01	AT2G21210	60.811	74	27	1	20	91	22	95	3.16e-29	100
MsG0480021384.01.T01	AT3G03820	54.255	94	37	2	2	92	6	96	3.48e-29	100
MsG0480021384.01.T01	AT3G03850	69.118	68	19	1	27	92	26	93	3.89e-29	100
MsG0480021384.01.T01	AT4G38850	58.621	87	23	2	8	91	13	89	6.49e-29	99.8
MsG0480021384.01.T01	AT3G03830	57.955	88	30	2	11	91	5	92	1.64e-28	99.0
MsG0480021384.01.T01	AT2G21210	60.811	74	27	1	20	91	89	162	1.82e-28	100
MsG0480021384.01.T01	AT4G34770	59.211	76	29	1	19	92	28	103	5.29e-28	97.8
MsG0480021384.01.T01	AT4G34810	56.250	80	29	3	17	90	38	117	1.05e-26	95.1
MsG0480021384.01.T01	AT4G38860	51.765	85	37	1	8	88	18	102	4.13e-26	93.2
MsG0480021384.01.T01	AT4G13790	51.765	85	33	3	8	90	13	91	1.52e-25	91.3
MsG0480021384.01.T01	AT4G34800	50.000	86	35	2	1	83	1	81	3.02e-25	90.5
MsG0480021384.01.T01	AT4G34760	49.425	87	37	2	8	88	19	104	3.64e-25	90.9
MsG0480021384.01.T01	AT2G21220	60.000	65	25	1	24	88	38	101	1.40e-24	89.4
MsG0480021384.01.T01	AT1G75580	47.253	91	39	2	8	90	18	107	1.74e-24	89.0
MsG0480021384.01.T01	AT1G19830	47.917	96	36	3	8	90	18	112	6.27e-23	85.5
MsG0480021384.01.T01	AT2G16580	46.591	88	39	2	8	88	19	105	5.20e-22	82.8
MsG0480021384.01.T01	AT4G36110	46.988	83	41	2	8	88	20	101	1.89e-21	81.3
MsG0480021384.01.T01	AT4G34790	56.716	67	26	2	26	89	39	105	3.43e-21	80.9
MsG0480021384.01.T01	AT4G34780	43.617	94	47	2	4	91	3	96	3.51e-21	80.9
MsG0480021384.01.T01	AT2G18010	54.688	64	28	1	25	88	47	109	8.23e-20	77.4
MsG0480021384.01.T01	AT5G66260	45.349	86	44	2	8	91	14	98	1.39e-19	76.3
MsG0480021384.01.T01	AT3G20220	48.684	76	37	2	15	89	36	110	1.93e-19	76.6
MsG0480021384.01.T01	AT3G43120	53.226	62	28	1	25	86	78	138	5.03e-17	71.6
MsG0480021384.01.T01	AT5G20810	50.725	69	33	1	25	93	78	145	1.45e-16	70.5
MsG0480021384.01.T01	AT5G20810	53.030	66	30	1	25	90	78	142	3.67e-16	70.1
MsG0480021384.01.T01	AT2G46690	41.176	85	45	3	13	93	10	93	1.10e-15	67.0
MsG0480021384.01.T01	AT3G51200	50.000	64	30	2	21	83	34	96	3.21e-15	65.5
MsG0480021384.01.T01	AT4G34750	45.312	64	34	1	25	88	36	98	7.55e-15	65.9
MsG0480021384.01.T01	AT4G34750	45.312	64	34	1	25	88	36	98	7.55e-15	65.9
MsG0480021384.01.T01	AT1G75590	41.176	85	45	2	4	84	16	99	2.27e-14	64.7
MsG0480021384.01.T01	AT2G45210	41.096	73	39	2	21	90	74	145	2.92e-14	64.3
MsG0480021384.01.T01	AT4G31320	49.231	65	32	1	26	90	80	143	4.65e-14	64.3
MsG0480021384.01.T01	AT5G10990	46.667	60	31	1	25	84	40	98	9.42e-14	62.8
MsG0480021384.01.T01	AT3G61900	42.857	70	36	2	25	91	29	97	1.12e-13	62.4
MsG0480021384.01.T01	AT2G24400	50.000	66	32	1	26	91	64	128	1.84e-13	62.8
MsG0480021384.01.T01	AT1G19840	47.059	68	33	2	19	84	32	98	1.85e-13	62.0
MsG0480021384.01.T01	AT3G60690	41.096	73	39	2	22	91	84	155	8.32e-13	60.8
MsG0480021384.01.T01	AT4G00880	50.794	63	28	2	26	86	28	89	9.14e-13	59.7
MsG0480021384.01.T01	AT2G37030	47.458	59	30	1	25	83	48	105	1.53e-12	59.3
MsG0480021384.01.T01	AT5G53590	32.653	98	60	2	1	93	21	117	1.66e-12	59.3
MsG0480021384.01.T01	AT4G09530	40.845	71	36	3	17	84	22	89	3.26e-11	55.1

Find 27 sgRNAs with CRISPR-Local

Find 29 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGTCAAATTGAGGAAGAGTT+TGG	0.355201	4:-57282728	MsG0480021384.01.T01:CDS
CTCAGCAAATCTTGAAATGA+AGG	0.381225	4:+57282749	None:intergenic
TTATAACATCTCGCATGAAC+GGG	0.464620	4:-57282649	MsG0480021384.01.T01:CDS
GCAAATCTTGAAATGAAGGT+TGG	0.509792	4:+57282753	None:intergenic
GGATATCTTGCAGTATATGT+TGG	0.514243	4:-57282818	MsG0480021384.01.T01:CDS
AGATTTGCTGAGTCAAATTG+AGG	0.517425	4:-57282738	MsG0480021384.01.T01:CDS
TATAAGTTGAAACATATCTT+CGG	0.520039	4:+57282667	None:intergenic
CATATCTTCGGTGCAAGGAA+TGG	0.521275	4:+57282679	None:intergenic
GGTTGGCTCAAGTATGATAC+GGG	0.521678	4:+57282770	None:intergenic
GTTTCCCTGGTATCATCAGA+AGG	0.529309	4:-57282892	MsG0480021384.01.T01:CDS
AGGTTGGCTCAAGTATGATA+CGG	0.529579	4:+57282769	None:intergenic
CTTATAACATCTCGCATGAA+CGG	0.532582	4:-57282650	MsG0480021384.01.T01:CDS
TATACTGCAAGATATCCCTT+TGG	0.554191	4:+57282824	None:intergenic
AGGAATGGTAAGGCCACCCA+TGG	0.560219	4:+57282694	None:intergenic
TGAAACATATCTTCGGTGCA+AGG	0.560974	4:+57282674	None:intergenic
GTTTGGATATGATCATCCCA+TGG	0.570301	4:-57282711	MsG0480021384.01.T01:CDS
GATGCCCTTCTGATGATACC+AGG	0.570722	4:+57282887	None:intergenic
AAAAGCTGTTGATGTGCCAA+AGG	0.571530	4:-57282840	MsG0480021384.01.T01:CDS
CTTCGGTGCAAGGAATGGTA+AGG	0.572529	4:+57282684	None:intergenic
AAAGCTGTTGATGTGCCAAA+GGG	0.580562	4:-57282839	MsG0480021384.01.T01:CDS
GGATATGATCATCCCATGGG+TGG	0.600402	4:-57282707	MsG0480021384.01.T01:CDS
TTTCCCTGGTATCATCAGAA+GGG	0.622803	4:-57282891	MsG0480021384.01.T01:CDS
TTTGGATATGATCATCCCAT+GGG	0.641263	4:-57282710	MsG0480021384.01.T01:CDS
ATGCCCTTCTGATGATACCA+GGG	0.658200	4:+57282888	None:intergenic
GGAATGGTAAGGCCACCCAT+GGG	0.680903	4:+57282695	None:intergenic
GTTGGCTCAAGTATGATACG+GGG	0.770839	4:+57282771	None:intergenic
ATGTTGGAGAGAAACAGACG+CGG	0.843088	4:-57282802	MsG0480021384.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TATAAGTTGAAACATATCTT+CGG	+	Chr4:57282881-57282900	None:intergenic	20.0%
	AGTCAAATTGAGGAAGAGTT+TGG	-	Chr4:57282817-57282836	MsG0480021384.01.T01:CDS	35.0%
	CTCAGCAAATCTTGAAATGA+AGG	+	Chr4:57282799-57282818	None:intergenic	35.0%
	CTTATAACATCTCGCATGAA+CGG	-	Chr4:57282895-57282914	MsG0480021384.01.T01:CDS	35.0%
	GCAAATCTTGAAATGAAGGT+TGG	+	Chr4:57282795-57282814	None:intergenic	35.0%
	GGATATCTTGCAGTATATGT+TGG	-	Chr4:57282727-57282746	MsG0480021384.01.T01:CDS	35.0%
	TATACTGCAAGATATCCCTT+TGG	+	Chr4:57282724-57282743	None:intergenic	35.0%
	TTATAACATCTCGCATGAAC+GGG	-	Chr4:57282896-57282915	MsG0480021384.01.T01:CDS	35.0%
	TTTGGATATGATCATCCCAT+GGG	-	Chr4:57282835-57282854	MsG0480021384.01.T01:CDS	35.0%
!!	AGATTTGCTGAGTCAAATTG+AGG	-	Chr4:57282807-57282826	MsG0480021384.01.T01:CDS	35.0%
	AAAAGCTGTTGATGTGCCAA+AGG	-	Chr4:57282705-57282724	MsG0480021384.01.T01:CDS	40.0%
	AAAGCTGTTGATGTGCCAAA+GGG	-	Chr4:57282706-57282725	MsG0480021384.01.T01:CDS	40.0%
	GTTTGGATATGATCATCCCA+TGG	-	Chr4:57282834-57282853	MsG0480021384.01.T01:CDS	40.0%
	TTTCCCTGGTATCATCAGAA+GGG	-	Chr4:57282654-57282673	MsG0480021384.01.T01:CDS	40.0%
!	AGGTTGGCTCAAGTATGATA+CGG	+	Chr4:57282779-57282798	None:intergenic	40.0%
!	TGAAACATATCTTCGGTGCA+AGG	+	Chr4:57282874-57282893	None:intergenic	40.0%
!!!	ATCAGAAGGGCATCTTTTTC+TGG	-	Chr4:57282667-57282686	MsG0480021384.01.T01:CDS	40.0%
!!!	CAACAGCTTTTGAAGCTGAT+CGG	+	Chr4:57282696-57282715	None:intergenic	40.0%
	ATGCCCTTCTGATGATACCA+GGG	+	Chr4:57282660-57282679	None:intergenic	45.0%
	ATGTTGGAGAGAAACAGACG+CGG	-	Chr4:57282743-57282762	MsG0480021384.01.T01:CDS	45.0%
	GTTTCCCTGGTATCATCAGA+AGG	-	Chr4:57282653-57282672	MsG0480021384.01.T01:CDS	45.0%
!	CATATCTTCGGTGCAAGGAA+TGG	+	Chr4:57282869-57282888	None:intergenic	45.0%
!	GGTTGGCTCAAGTATGATAC+GGG	+	Chr4:57282778-57282797	None:intergenic	45.0%
!	GTTGGCTCAAGTATGATACG+GGG	+	Chr4:57282777-57282796	None:intergenic	45.0%
	GATGCCCTTCTGATGATACC+AGG	+	Chr4:57282661-57282680	None:intergenic	50.0%
	GGATATGATCATCCCATGGG+TGG	-	Chr4:57282838-57282857	MsG0480021384.01.T01:CDS	50.0%
!	CTTCGGTGCAAGGAATGGTA+AGG	+	Chr4:57282864-57282883	None:intergenic	50.0%
	AGGAATGGTAAGGCCACCCA+TGG	+	Chr4:57282854-57282873	None:intergenic	55.0%
	GGAATGGTAAGGCCACCCAT+GGG	+	Chr4:57282853-57282872	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
Chr4	gene	57282643	57282924	57282643	ID=MsG0480021384.01;Name=MsG0480021384.01
Chr4	mRNA	57282643	57282924	57282643	ID=MsG0480021384.01.T01;Parent=MsG0480021384.01;Name=MsG0480021384.01.T01;_AED=0.50;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|93
Chr4	exon	57282643	57282924	57282643	ID=MsG0480021384.01.T01:exon:7233;Parent=MsG0480021384.01.T01
Chr4	CDS	57282643	57282924	57282643	ID=MsG0480021384.01.T01:cds;Parent=MsG0480021384.01.T01

Gene Sequence	>MsG0480021384.01.T01 ATGGGTTTTCGTTTCCCTGGTATCATCAGAAGGGCATCTTTTTCTGGAAACCGATCAGCTTCAAAAGCTGTTGATGTGCCAAAGGGATATCTTGCAGTATATGTTGGAGAGAAACAGACGCGGTATGTGATCCCCGTATCATACTTGAGCCAACCTTCATTTCAAGATTTGCTGAGTCAAATTGAGGAAGAGTTTGGATATGATCATCCCATGGGTGGCCTTACCATTCCTTGCACCGAAGATATGTTTCAACTTATAACATCTCGCATGAACGGGCAATGA
Protein sequence	>MsG0480021384.01.T01 MGFRFPGIIRRASFSGNRSASKAVDVPKGYLAVYVGEKQTRYVIPVSYLSQPSFQDLLSQIEEEFGYDHPMGGLTIPCTEDMFQLITSRMNGQ*