Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480021642.01.T01 | XP_003600261.1 | 96.679 | 542 | 18 | 0 | 1 | 542 | 1 | 542 | 0 | 1092 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000089.01 | MsG0480021642.01 | 0.842569 | 2.386761e-58 | 3.633791e-55 |
| MsG0180000222.01 | MsG0480021642.01 | 0.818082 | 2.359978e-52 | 1.749169e-49 |
| MsG0180000585.01 | MsG0480021642.01 | 0.817418 | 3.332333e-52 | 2.425502e-49 |
| MsG0180002912.01 | MsG0480021642.01 | 0.805039 | 1.618069e-49 | 8.473118e-47 |
| MsG0180004462.01 | MsG0480021642.01 | 0.833507 | 5.124713e-56 | 5.913922e-53 |
| MsG0180004752.01 | MsG0480021642.01 | 0.819254 | 1.279068e-52 | 9.792630e-50 |
| MsG0180004762.01 | MsG0480021642.01 | 0.825653 | 4.167836e-54 | 3.822351e-51 |
| MsG0180005172.01 | MsG0480021642.01 | 0.850976 | 1.198395e-60 | 2.389338e-57 |
| MsG0180005331.01 | MsG0480021642.01 | 0.877884 | 4.390348e-69 | 2.244913e-65 |
| MsG0180005813.01 | MsG0480021642.01 | 0.819781 | 9.700592e-53 | 7.536945e-50 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480021642.01.T01 | MTR_3g056110 | 96.679 | 542 | 18 | 0 | 1 | 542 | 1 | 542 | 0.0 | 1092 |
| MsG0480021642.01.T01 | MTR_5g094450 | 67.942 | 549 | 152 | 9 | 1 | 542 | 1 | 532 | 0.0 | 736 |
| MsG0480021642.01.T01 | MTR_5g097480 | 57.713 | 551 | 217 | 6 | 1 | 542 | 1 | 544 | 0.0 | 653 |
| MsG0480021642.01.T01 | MTR_4g133660 | 49.020 | 561 | 247 | 9 | 1 | 541 | 1 | 542 | 6.34e-180 | 518 |
| MsG0480021642.01.T01 | MTR_2g082090 | 59.140 | 372 | 152 | 0 | 170 | 541 | 207 | 578 | 1.21e-169 | 493 |
| MsG0480021642.01.T01 | MTR_2g026250 | 46.753 | 539 | 230 | 7 | 56 | 542 | 63 | 596 | 2.11e-166 | 485 |
| MsG0480021642.01.T01 | MTR_1g029420 | 50.000 | 370 | 185 | 0 | 173 | 542 | 223 | 592 | 3.66e-141 | 420 |
| MsG0480021642.01.T01 | MTR_3g089055 | 49.344 | 381 | 192 | 1 | 162 | 542 | 186 | 565 | 1.32e-138 | 413 |
| MsG0480021642.01.T01 | MTR_3g089055 | 49.344 | 381 | 192 | 1 | 162 | 542 | 191 | 570 | 1.42e-138 | 413 |
| MsG0480021642.01.T01 | MTR_7g027190 | 34.120 | 551 | 295 | 13 | 21 | 542 | 162 | 673 | 3.66e-79 | 262 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480021642.01.T01 | AT1G50600 | 56.044 | 546 | 216 | 7 | 1 | 542 | 72 | 597 | 0.0 | 593 |
| MsG0480021642.01.T01 | AT1G50600 | 56.044 | 546 | 216 | 7 | 1 | 542 | 76 | 601 | 0.0 | 593 |
| MsG0480021642.01.T01 | AT1G50600 | 56.044 | 546 | 216 | 7 | 1 | 542 | 1 | 526 | 0.0 | 592 |
| MsG0480021642.01.T01 | AT5G48150 | 64.554 | 426 | 148 | 2 | 118 | 542 | 67 | 490 | 0.0 | 582 |
| MsG0480021642.01.T01 | AT5G48150 | 64.554 | 426 | 148 | 2 | 118 | 542 | 67 | 490 | 0.0 | 582 |
| MsG0480021642.01.T01 | AT5G48150 | 64.554 | 426 | 148 | 2 | 118 | 542 | 67 | 490 | 0.0 | 582 |
| MsG0480021642.01.T01 | AT5G48150 | 64.554 | 426 | 148 | 2 | 118 | 542 | 67 | 490 | 0.0 | 582 |
| MsG0480021642.01.T01 | AT2G04890 | 59.179 | 414 | 162 | 1 | 129 | 542 | 7 | 413 | 0.0 | 521 |
| MsG0480021642.01.T01 | AT4G17230 | 49.183 | 551 | 245 | 10 | 1 | 542 | 1 | 525 | 3.53e-171 | 495 |
| MsG0480021642.01.T01 | AT4G17230 | 49.183 | 551 | 245 | 10 | 1 | 542 | 1 | 525 | 3.53e-171 | 495 |
Find 117 sgRNAs with CRISPR-Local
Find 129 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAATTGGACAAGGAACTCAG+TGG | 0.723091 | 4:+61167511 | MsG0480021642.01.T01:CDS |
| AAATTTCTTACGGCTCTGAC+AGG | 0.480661 | 4:+61166677 | MsG0480021642.01.T01:CDS |
| AACATCAATTGATTCATAAA+CGG | 0.428207 | 4:-61167958 | None:intergenic |
| AACATGGAGATGGTATCTAG+AGG | 0.612916 | 4:+61167141 | MsG0480021642.01.T01:CDS |
| AAGGATACACTCTAACAGCA+TGG | 0.621994 | 4:-61167698 | None:intergenic |
| AAGGCATAAGATGAGTGAGC+TGG | 0.578063 | 4:+61167014 | MsG0480021642.01.T01:CDS |
| AAGTAAGAATCCTCCGCCTC+AGG | 0.498080 | 4:-61166967 | None:intergenic |
| AATCTTCTCGAGAGCTACCG+TGG | 0.627998 | 4:+61168176 | MsG0480021642.01.T01:CDS |
| AATTGATGTTGTTCTTCCAA+GGG | 0.531808 | 4:+61167971 | MsG0480021642.01.T01:CDS |
| AATTGGACAAGGAACTCAGT+GGG | 0.647751 | 4:+61167512 | MsG0480021642.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AATTGATGTTGTTCTTCCAA+GGG | + | Chr4:61167971-61167990 | MsG0480021642.01.T01:CDS | 30.0% | |
| ACAAATTCAAGTGAATGTTG+CGG | - | Chr4:61166937-61166956 | None:intergenic | 30.0% | |
| ATGATATCGAGACAATTGAA+TGG | + | Chr4:61167211-61167230 | MsG0480021642.01.T01:CDS | 30.0% | |
| ATTGTCTAGATTCTCAATTG+AGG | - | Chr4:61166746-61166765 | None:intergenic | 30.0% | |
| CATTCACTTGAATTTGTCAA+TGG | + | Chr4:61166940-61166959 | MsG0480021642.01.T01:CDS | 30.0% | |
| CTTGATTGAAGAATTGATGA+AGG | - | Chr4:61168156-61168175 | None:intergenic | 30.0% | |
| TTCATAAAGTACATGCATGT+AGG | - | Chr4:61167397-61167416 | None:intergenic | 30.0% | |
| TTGTCTAGATTCTCAATTGA+GGG | - | Chr4:61166745-61166764 | None:intergenic | 30.0% | |
| AGAGAAGTGGAAGAAAAACA+TGG | + | Chr4:61167125-61167144 | MsG0480021642.01.T01:CDS | 35.0% | |
| AGCAGATTCTTCTTGAACTT+TGG | - | Chr4:61167088-61167107 | None:intergenic | 35.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 61166655 | 61168283 | 61166655 | ID=MsG0480021642.01;Name=MsG0480021642.01 |
| Chr4 | mRNA | 61166655 | 61168283 | 61166655 | ID=MsG0480021642.01.T01;Parent=MsG0480021642.01;Name=MsG0480021642.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|542 |
| Chr4 | exon | 61166655 | 61168283 | 61166655 | ID=MsG0480021642.01.T01:exon:8660;Parent=MsG0480021642.01.T01 |
| Chr4 | CDS | 61166655 | 61168283 | 61166655 | ID=MsG0480021642.01.T01:cds;Parent=MsG0480021642.01.T01 |
| Gene Sequence |
>MsG0480021642.01.T01 ATGCAAACATCTCAGAAACATGAAATTTCTTACGGCTCTGACAGGTTCTACGTTGAGCCTGTACAGAATCTCGAGTCGTATTGCTTACCCTCAATTGAGAATCTAGACAATTATTCTTCCTCTGATAACAGCTGCCAAACATTCTATCCTTCTCATCAAACTCTAGAACAATACAACAACCTTGAATCTGCTTCAACAAGCAACAACAGTTTACCTTACCAAAATTCTCCCTCTACTCACGGTTTCTCACCTAACAATAGTCCAGGTTCTACGTTAAGGCCGCAACATTCACTTGAATTTGTCAATGGTTCACCTGAGGCGGAGGATTCTTACTTGACATATCATGACCATGATGACCTAAGGCATAAGATGAGTGAGCTGGAATCTGTTATGCTCGGACCTAACGTAGAGATGCTAGAGATGTATGATACCAAAGTTCAAGAAGAATCTGCTTCGTTCTTATTAGAGGCAGAGAAGTGGAAGAAAAACATGGAGATGGTATCTAGAGGGGATTTGAAAGAGATGCTTTATACTTGTGCAAAAGCAGTGGATGTGAATGATATCGAGACAATTGAATGGATGGTAACTGAGTTACGCAAAATAGTATCCGTTTCAGGCAGTCCAATCGAACGATTGGGAGCGTACATGTTGGAGGCACTTGTTTCAAAGATTGCCTCTTCTGGAAGTACAATCTACAAATCGTTAAAATGCAGCGAGCCTACTGGTAATGAACTGCTTTCCTACATGCATGTACTTTATGAAATATGTCCGTACTTTAAATTCGGATATATGTCTGCAAATGGAGCCATCGCGGAAGCTATGAAGGAGGAAAATGAAGTCCACATAATTGATTTTCAAATTGGACAAGGAACTCAGTGGGTGAGCCTAATCCAGGCTCTTGCTCGCCGCCCTGGAGGACCTCCAAAGATTAGAATAACAGGTATTGATGACTCTTATTCATCGTATGCCCGCGGTGGGGGCGTTGATATAGTAGGGGAGAAGTTATCCGCGCTTGCGCAGTCATGTCATGTACCATTTGAGTTCCATGCTGTTAGAGTGTATCCTTCTGAGGTGCGACTCGAAGACTTTGAACTTCGTCCTAATGAAGCTGTGGCGGTGAACTTTGCCATTATGCTACATCATGTACCAGATGAAAGTGTGACCATTCATAATCATCGTGACAGGTTGCTGCGATTGGCTAAGCACATGTCTCCTAAGGTGGTGACTCTAGTTGAGCAAGAATTCAACACCAATAACGCTCCGTTCTTGCAGCGTTTCGTCGAGACAATGAACTACTACTCAGCCGTTTATGAATCAATTGATGTTGTTCTTCCAAGGGATCACAAAGAGAGGATTAATGTCGAACAGCACTGTTTGGCTCGTGAAGTTGTGAACTTAGTAGCTTGCGAAGGGGAAGAAAGAGTGGAACGTCACGAGCTTCTGAGTAAGTGGAAAAAGCGTTTCACGATGGCTGGATTTACGCCTTATCCATTGAGCTCCTTCATCAATTCTTCAATCAAGAATCTTCTCGAGAGCTACCGTGGACATTACACCCTAGAAGAGAGAGATGGTGCACTGTTTCTGGGTTGGATGAATCAAGATCTTATTGTTTCTTGTGCTTGGAGATGA |
| Protein sequence |
>MsG0480021642.01.T01 MQTSQKHEISYGSDRFYVEPVQNLESYCLPSIENLDNYSSSDNSCQTFYPSHQTLEQYNNLESASTSNNSLPYQNSPSTHGFSPNNSPGSTLRPQHSLEFVNGSPEAEDSYLTYHDHDDLRHKMSELESVMLGPNVEMLEMYDTKVQEESASFLLEAEKWKKNMEMVSRGDLKEMLYTCAKAVDVNDIETIEWMVTELRKIVSVSGSPIERLGAYMLEALVSKIASSGSTIYKSLKCSEPTGNELLSYMHVLYEICPYFKFGYMSANGAIAEAMKEENEVHIIDFQIGQGTQWVSLIQALARRPGGPPKIRITGIDDSYSSYARGGGVDIVGEKLSALAQSCHVPFEFHAVRVYPSEVRLEDFELRPNEAVAVNFAIMLHHVPDESVTIHNHRDRLLRLAKHMSPKVVTLVEQEFNTNNAPFLQRFVETMNYYSAVYESIDVVLPRDHKERINVEQHCLAREVVNLVACEGEERVERHELLSKWKKRFTMAGFTPYPLSSFINSSIKNLLESYRGHYTLEERDGALFLGWMNQDLIVSCAWR* |
