Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004762.01.T01 | XP_003591335.1 | 97.949 | 390 | 8 | 0 | 1 | 390 | 1 | 390 | 0 | 785 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048177.01 | MsG0180004762.01 | 0.849259 | 3.626901e-60 | 6.836807e-57 |
| MsG0080048178.01 | MsG0180004762.01 | 0.809941 | 1.474964e-50 | 8.776024e-48 |
| MsG0080048205.01 | MsG0180004762.01 | 0.802317 | 5.942647e-49 | 2.902227e-46 |
| MsG0080048915.01 | MsG0180004762.01 | 0.810425 | 1.160265e-50 | 6.991178e-48 |
| MsG0180000041.01 | MsG0180004762.01 | 0.819822 | 9.490746e-53 | 7.382189e-50 |
| MsG0180000267.01 | MsG0180004762.01 | 0.801009 | 1.101935e-48 | 5.206208e-46 |
| MsG0180000293.01 | MsG0180004762.01 | 0.812811 | 3.513943e-51 | 2.256056e-48 |
| MsG0180000585.01 | MsG0180004762.01 | 0.807200 | 5.677726e-50 | 3.142872e-47 |
| MsG0180001053.01 | MsG0180004762.01 | 0.817288 | 3.564781e-52 | 2.585299e-49 |
| MsG0180001921.01 | MsG0180004762.01 | 0.821229 | 4.511894e-53 | 3.649457e-50 |
PPI
| Gene1 | Gene2 | Type |
|---|---|---|
| MsG0080048977.01 | MsG0180004762.01 | PPI |
| MsG0180004762.01 | MsG0780038271.01 | PPI |
| MsG0380017457.01 | MsG0180004762.01 | PPI |
| MsG0380017494.01 | MsG0180004762.01 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004762.01.T01 | MTR_1g086350 | 97.949 | 390 | 8 | 0 | 1 | 390 | 1 | 390 | 0.0 | 785 |
| MsG0180004762.01.T01 | MTR_1g086350 | 93.846 | 390 | 8 | 1 | 1 | 390 | 1 | 374 | 0.0 | 744 |
| MsG0180004762.01.T01 | MTR_1g086350 | 98.535 | 273 | 4 | 0 | 118 | 390 | 16 | 288 | 0.0 | 556 |
| MsG0180004762.01.T01 | MTR_2g033000 | 56.589 | 387 | 149 | 3 | 1 | 387 | 1 | 368 | 1.59e-152 | 435 |
| MsG0180004762.01.T01 | MTR_4g123080 | 55.385 | 390 | 162 | 4 | 1 | 387 | 1 | 381 | 1.71e-151 | 433 |
| MsG0180004762.01.T01 | MTR_4g123080 | 54.872 | 390 | 146 | 5 | 1 | 387 | 1 | 363 | 6.31e-150 | 428 |
| MsG0180004762.01.T01 | MTR_5g024340 | 50.943 | 318 | 134 | 3 | 73 | 386 | 79 | 378 | 4.72e-110 | 328 |
| MsG0180004762.01.T01 | MTR_4g116420 | 47.904 | 334 | 146 | 6 | 62 | 385 | 68 | 383 | 2.72e-99 | 300 |
| MsG0180004762.01.T01 | MTR_3g107880 | 46.588 | 337 | 155 | 6 | 56 | 385 | 1 | 319 | 3.40e-94 | 285 |
| MsG0180004762.01.T01 | MTR_3g107880 | 47.230 | 343 | 154 | 8 | 50 | 385 | 55 | 377 | 1.56e-93 | 285 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004762.01.T01 | AT3G62260 | 61.224 | 392 | 137 | 8 | 1 | 386 | 1 | 383 | 9.31e-166 | 469 |
| MsG0180004762.01.T01 | AT3G62260 | 61.224 | 392 | 138 | 8 | 1 | 386 | 1 | 384 | 1.90e-165 | 469 |
| MsG0180004762.01.T01 | AT3G62260 | 68.243 | 296 | 91 | 2 | 92 | 386 | 1 | 294 | 1.19e-149 | 425 |
| MsG0180004762.01.T01 | AT1G48040 | 58.376 | 394 | 138 | 11 | 1 | 386 | 7 | 382 | 3.42e-145 | 417 |
| MsG0180004762.01.T01 | AT3G17250 | 55.495 | 364 | 142 | 10 | 34 | 386 | 68 | 422 | 1.06e-129 | 379 |
| MsG0180004762.01.T01 | AT2G33700 | 53.605 | 319 | 125 | 3 | 73 | 386 | 78 | 378 | 4.78e-117 | 345 |
| MsG0180004762.01.T01 | AT3G51470 | 49.524 | 315 | 132 | 4 | 74 | 384 | 23 | 314 | 1.65e-105 | 314 |
| MsG0180004762.01.T01 | AT3G51470 | 49.524 | 315 | 132 | 4 | 74 | 384 | 67 | 358 | 6.24e-105 | 314 |
| MsG0180004762.01.T01 | AT2G25620 | 46.087 | 345 | 157 | 7 | 53 | 386 | 63 | 389 | 5.02e-95 | 290 |
| MsG0180004762.01.T01 | AT2G25620 | 46.087 | 345 | 157 | 7 | 53 | 386 | 63 | 389 | 5.02e-95 | 290 |
Find 118 sgRNAs with CRISPR-Local
Find 211 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAACTACCGGAACGGATGG+AGG | 0.545279 | 1:+82360842 | None:intergenic |
| AAAATTGGGTGGCTATGTTG+AGG | 0.666115 | 1:-82359906 | MsG0180004762.01.T01:CDS |
| AAACTACCGGAACGGATGGA+GGG | 0.556241 | 1:+82360843 | None:intergenic |
| AAAGCTCATTACCCCATAAA+AGG | 0.322934 | 1:+82360719 | None:intergenic |
| AAAGGTGAGGGAGTACCCCT+AGG | 0.611278 | 1:+82359821 | None:intergenic |
| AAATAAATGTCGTGTCATAG+TGG | 0.573103 | 1:+82359251 | None:intergenic |
| AAATCCCTGGCGCACTTCTC+TGG | 0.265688 | 1:+82359656 | None:intergenic |
| AACTACCGGAACGGATGGAG+GGG | 0.553531 | 1:+82360844 | None:intergenic |
| AACTCAGGATCTGCAATCAA+AGG | 0.595994 | 1:+82359803 | None:intergenic |
| AATCCCTGGCGCACTTCTCT+GGG | 0.496285 | 1:+82359657 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAACAGAAGAAATTCTCGAA+AGG | + | Chr1:82359617-82359636 | None:intergenic | 30.0% | |
| AAATAAATGTCGTGTCATAG+TGG | + | Chr1:82362136-82362155 | None:intergenic | 30.0% | |
| AACAGAAGAAATTCTCGAAA+GGG | + | Chr1:82359616-82359635 | None:intergenic | 30.0% | |
| ACATGGAAGATGAACATATA+AGG | - | Chr1:82360587-82360606 | MsG0180004762.01.T01:CDS | 30.0% | |
| ACTTCTAATATGCTTCTTTC+AGG | - | Chr1:82361339-82361358 | MsG0180004762.01.T01:intron | 30.0% | |
| CAAAAGAAAAGCCTTTCTAA+GGG | + | Chr1:82360892-82360911 | None:intergenic | 30.0% | |
| CAAAGAAACTCAATTTCAAC+TGG | + | Chr1:82359984-82360003 | None:intergenic | 30.0% | |
| CAAATGTATCAGGTATTTGA+TGG | - | Chr1:82360744-82360763 | MsG0180004762.01.T01:CDS | 30.0% | |
| GAAGTCAGTTAAACAAACAA+AGG | + | Chr1:82361324-82361343 | None:intergenic | 30.0% | |
| GTTTATAACCTTCATTGTTG+AGG | - | Chr1:82360140-82360159 | MsG0180004762.01.T01:intron | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 82359210 | 82362196 | 82359210 | ID=MsG0180004762.01;Name=MsG0180004762.01 |
| Chr1 | mRNA | 82359210 | 82362196 | 82359210 | ID=MsG0180004762.01.T01;Parent=MsG0180004762.01;Name=MsG0180004762.01.T01;_AED=0.33;_eAED=0.33;_QI=224|1|1|1|1|1|4|259|390 |
| Chr1 | exon | 82359210 | 82360045 | 82359210 | ID=MsG0180004762.01.T01:exon:27274;Parent=MsG0180004762.01.T01 |
| Chr1 | exon | 82360415 | 82360650 | 82360415 | ID=MsG0180004762.01.T01:exon:27273;Parent=MsG0180004762.01.T01 |
| Chr1 | exon | 82360731 | 82360928 | 82360731 | ID=MsG0180004762.01.T01:exon:27272;Parent=MsG0180004762.01.T01 |
| Chr1 | exon | 82361811 | 82362196 | 82361811 | ID=MsG0180004762.01.T01:exon:27271;Parent=MsG0180004762.01.T01 |
| Chr1 | five_prime_UTR | 82361973 | 82362196 | 82361973 | ID=MsG0180004762.01.T01:five_prime_utr;Parent=MsG0180004762.01.T01 |
| Chr1 | CDS | 82361811 | 82361972 | 82361811 | ID=MsG0180004762.01.T01:cds;Parent=MsG0180004762.01.T01 |
| Chr1 | CDS | 82360731 | 82360928 | 82360731 | ID=MsG0180004762.01.T01:cds;Parent=MsG0180004762.01.T01 |
| Chr1 | CDS | 82360415 | 82360650 | 82360415 | ID=MsG0180004762.01.T01:cds;Parent=MsG0180004762.01.T01 |
| Chr1 | CDS | 82359469 | 82360045 | 82359469 | ID=MsG0180004762.01.T01:cds;Parent=MsG0180004762.01.T01 |
| Chr1 | three_prime_UTR | 82359210 | 82359468 | 82359210 | ID=MsG0180004762.01.T01:three_prime_utr;Parent=MsG0180004762.01.T01 |
| Gene Sequence |
>MsG0180004762.01.T01 ATGGTGGCTGAAGCAGAGGTTGTTGTGTGTCAACAAAGCGTGGATGTGAAGTATTTTCCGAATAAAGGGAGCAATAATCACGAGATCGGTGATGTAGATTCGAATTTTGATCGAGTTCGTGTTTCTGAATCAGTTTCTGCTGAGTTACTTACTACAAAAGAAGATGTTAAGACTACAGATATAACCTCGGAAGCTGGTCTTGAATCTGCGGTTCTGCAATTTGTCCCCTCCATCCGTTCCGGTAGTTTTGCCGACATTGGGCCACGTAGATACATGGAAGATGAACATATAAGGATAGATGATTTGTCATCTCATCTGGGATCACAATACAATTTTCCTAAACCAAGTGCCTTTTATGGGGTATTTGATGGTCACGGAGGACCTGAAGCTGCAGCGTATATAAGAAAAAATGTTCTAAAATTCTTCTTTGAGGATGTCAATTTCCCTCAGATATCTGAAGTTGATAATGTATTTTTACAAGAGGTAGAGAATTCCCTTAGAAAGGCTTTTCTTTTGGCAGACTCAGCTTTGGCTGATGATAGCAATGTGAACACTTCATCAGGGACCACAGCTCTTACTGCTTTGATATTTGGAAGGCTTCTAATGGTGGCAAATGCCGGTGACTGCCGAGCAGTTCTCAGTCGTAAAGGAGAGGCCATTGACATGTCTCAAGATCATAGGCCTATTTATCCATCAGAGCGGAGGCGAGTTGAAAAATTGGGTGGCTATGTTGAGGATGGTTATCTTAATGGCGTTTTATCAGTTACGAGAGCACTAGGGGACTGGGACATGAAGTTACCTAGGGGTACTCCCTCACCTTTGATTGCAGATCCTGAGTTCCACCAAGTGGTTCTAACAGAGGATGACGAATTCCTCATCATAGGATGCGACGGCATCTGGGATGTGATGACAAGTCAGCATGCTGTTAGCCTTGTTCGTAAAGGTTTGAGGAGGCATGATGACCCAGAGAAGTGCGCCAGGGATTTAGTTATGGAGGCATTACGGCTCAACACATTTGACAATCTTACAGTGATCATCATATGCCTCTCCTCCCTTGACCATGGGGAGTCAGAGCCATCACCACCTCCCCAACGGAAATTGAGATGCTGTAGCCTCTCTGCAGAGGCTCTTTGTAGCTTGAGAAGCTTGTTGGAAGGCAATGCTAGCAACTGA |
| Protein sequence |
>MsG0180004762.01.T01 MVAEAEVVVCQQSVDVKYFPNKGSNNHEIGDVDSNFDRVRVSESVSAELLTTKEDVKTTDITSEAGLESAVLQFVPSIRSGSFADIGPRRYMEDEHIRIDDLSSHLGSQYNFPKPSAFYGVFDGHGGPEAAAYIRKNVLKFFFEDVNFPQISEVDNVFLQEVENSLRKAFLLADSALADDSNVNTSSGTTALTALIFGRLLMVANAGDCRAVLSRKGEAIDMSQDHRPIYPSERRRVEKLGGYVEDGYLNGVLSVTRALGDWDMKLPRGTPSPLIADPEFHQVVLTEDDEFLIIGCDGIWDVMTSQHAVSLVRKGLRRHDDPEKCARDLVMEALRLNTFDNLTVIIICLSSLDHGESEPSPPPQRKLRCCSLSAEALCSLRSLLEGNASN* |
