AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480021873.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480021873.01.T01 MTR_4g478280 92.228 193 15 0 1 193 275 467 2.48e-128 369
MsG0480021873.01.T01 MTR_4g478260 92.228 193 15 0 1 193 283 475 2.28e-121 352
MsG0480021873.01.T01 MTR_4g478220 88.601 193 22 0 1 193 275 467 1.08e-113 332
MsG0480021873.01.T01 MTR_4g078600 80.829 193 37 0 1 193 275 467 4.53e-112 328
MsG0480021873.01.T01 MTR_4g078390 75.130 193 45 1 1 193 204 393 1.69e-106 311
MsG0480021873.01.T01 MTR_4g078390 75.130 193 45 1 1 193 273 462 1.74e-105 311
MsG0480021873.01.T01 MTR_4g078440 56.995 193 83 0 1 193 246 438 6.28e-84 255
MsG0480021873.01.T01 MTR_4g078480 57.812 192 81 0 1 192 269 460 1.31e-80 247
MsG0480021873.01.T01 MTR_4g078417 57.778 180 76 0 1 180 270 449 1.36e-79 244
MsG0480021873.01.T01 MTR_4g078413 55.497 191 85 0 1 191 270 460 1.11e-77 240
MsG0480021873.01.T01 MTR_7g009960 51.031 194 94 1 1 193 272 465 5.09e-71 223
MsG0480021873.01.T01 MTR_0024s0190 51.042 192 93 1 1 191 211 402 4.30e-66 208
MsG0480021873.01.T01 MTR_0024s0170 47.423 194 99 1 1 191 274 467 3.74e-61 197
MsG0480021873.01.T01 MTR_2055s0010 91.209 91 8 0 102 192 1 91 1.05e-56 174
MsG0480021873.01.T01 MTR_7g009960 53.378 148 68 1 1 147 272 419 4.01e-53 175
MsG0480021873.01.T01 MTR_7g078060 44.920 187 102 1 1 187 180 365 9.25e-53 173
MsG0480021873.01.T01 MTR_7g078060 44.920 187 102 1 1 187 272 457 9.23e-52 173
MsG0480021873.01.T01 MTR_4g078390 64.754 122 40 1 1 122 204 322 5.44e-50 164
MsG0480021873.01.T01 MTR_4g078390 64.754 122 40 1 1 122 273 391 3.27e-49 164
MsG0480021873.01.T01 MTR_5g004770 28.655 171 120 2 14 183 215 384 8.31e-19 83.2
MsG0480021873.01.T01 MTR_1g008160 25.926 162 117 1 15 176 145 303 7.13e-15 72.0
MsG0480021873.01.T01 MTR_1g008160 25.926 162 117 1 15 176 299 457 1.60e-14 71.2
MsG0480021873.01.T01 MTR_1g008160 25.926 162 117 1 15 176 255 413 2.03e-14 70.9
MsG0480021873.01.T01 MTR_1g008160 25.564 133 96 1 15 147 299 428 4.28e-11 61.2
MsG0480021873.01.T01 MTR_1g008160 25.564 133 96 1 15 147 255 384 5.21e-11 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480021873.01.T01 AT3G49190 26.923 156 111 1 9 164 308 460 4.92e-16 75.9
MsG0480021873.01.T01 AT5G12420 24.845 161 117 2 19 179 304 460 6.32e-16 75.5
MsG0480021873.01.T01 AT5G22490 26.590 173 123 2 4 176 289 457 1.19e-15 74.7
MsG0480021873.01.T01 AT5G53390 27.152 151 107 1 6 156 293 440 1.58e-15 74.3
MsG0480021873.01.T01 AT3G49210 26.344 186 133 2 9 193 329 511 1.23e-14 71.6
MsG0480021873.01.T01 AT5G16350 24.571 175 128 2 5 179 272 442 4.22e-14 70.1
MsG0480021873.01.T01 AT3G49200 26.061 165 118 2 15 179 326 486 4.66e-14 70.1
MsG0480021873.01.T01 AT5G12420 27.119 118 83 1 19 136 304 418 1.25e-13 68.9
MsG0480021873.01.T01 AT5G16350 24.845 161 117 2 19 179 306 462 1.34e-13 68.9
MsG0480021873.01.T01 AT2G38995 23.316 193 142 3 3 193 101 289 3.04e-12 64.3
MsG0480021873.01.T01 AT2G38995 23.316 193 142 3 3 193 222 410 4.95e-12 63.9
MsG0480021873.01.T01 AT2G38995 23.316 193 142 3 3 193 291 479 6.27e-12 63.9

Find 38 sgRNAs with CRISPR-Local

Find 149 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGGCAAACCATCCGGTAAA+TGG 0.244375 4:-65424356 MsG0480021873.01.T01:CDS
CCAACCCTCTTGAGTTTGTT+TGG 0.247573 4:-65424768 MsG0480021873.01.T01:CDS
TGTAGCTTTGCTCATGGATT+TGG 0.268046 4:-65424695 MsG0480021873.01.T01:CDS
AATCGCTATGAAAACCAAAA+AGG 0.275963 4:-65422503 MsG0480021873.01.T01:CDS
AACCAGAGTGCAAAGGTCTT+TGG 0.282055 4:-65424849 MsG0480021873.01.T01:CDS
ACCAGAGTGCAAAGGTCTTT+GGG 0.288027 4:-65424848 MsG0480021873.01.T01:CDS
ATTCCTGATTGGCTTACTTT+AGG 0.303836 4:+65424793 None:intergenic
AAAATAATCTACAACACAAT+TGG 0.333891 4:-65424431 MsG0480021873.01.T01:CDS
GATTGGCTTACTTTAGGTAT+TGG 0.353212 4:+65424799 None:intergenic
ATGCTCAACACAAGAAATAT+TGG 0.356933 4:-65424901 MsG0480021873.01.T01:CDS
TTTGCATTTCCTTCACTGAT+TGG 0.357490 4:+65424872 None:intergenic
GTGAAATACAATCCATTTAC+CGG 0.402011 4:+65424344 None:intergenic
CTTTCTCTTGATTAGTTCAC+GGG 0.438535 4:+65424741 None:intergenic
CCCCAAAGACCTTTGCACTC+TGG 0.446961 4:+65424847 None:intergenic
GATTTGGAGATGAGATTAAG+AGG 0.447085 4:-65424679 MsG0480021873.01.T01:CDS
AAATAATCTACAACACAATT+GGG 0.449065 4:-65424430 MsG0480021873.01.T01:CDS
TTATCTTGTAGCTTTGCTCA+TGG 0.454159 4:-65424701 MsG0480021873.01.T01:CDS
ATACCTAAAGTAAGCCAATC+AGG 0.457300 4:-65424796 MsG0480021873.01.T01:CDS
TCTTTCTCTTGATTAGTTCA+CGG 0.458319 4:+65424740 None:intergenic
GGATTGTATTTCACTGCGAC+AGG 0.471296 4:-65424335 MsG0480021873.01.T01:CDS
GAGGGTTGGAGATTCCTGAT+TGG 0.498647 4:+65424782 None:intergenic
AATGACTTTGGCAAACCATC+CGG 0.504375 4:-65424363 MsG0480021873.01.T01:CDS
TATCATATTCAAAGCAGCTA+TGG 0.563442 4:-65422431 MsG0480021873.01.T01:CDS
CCAAACAAACTCAAGAGGGT+TGG 0.570642 4:+65424768 None:intergenic
TTGTATTTCACTGCGACAGG+TGG 0.573211 4:-65424332 MsG0480021873.01.T01:CDS
AATACAATCCATTTACCGGA+TGG 0.574178 4:+65424348 None:intergenic
CCAGAGTGCAAAGGTCTTTG+GGG 0.576472 4:-65424847 MsG0480021873.01.T01:CDS
CTCAACACAAGAAATATTGG+AGG 0.579193 4:-65424898 MsG0480021873.01.T01:CDS
GAATTCCAAACAAACTCAAG+AGG 0.614444 4:+65424763 None:intergenic
CACTGCGACAGGTGGACCTG+AGG 0.631902 4:-65424324 MsG0480021873.01.T01:intron
AAGAGCAAATTCCACAGCAG+TGG 0.634903 4:-65424926 MsG0480021873.01.T01:CDS
TGGAGGTTACCAATCAGTGA+AGG 0.641441 4:-65424881 MsG0480021873.01.T01:CDS
TTAGGCTGTACATGGAAGAG+AGG 0.644200 4:-65424963 None:intergenic
CAGAGTGCAAAGGTCTTTGG+GGG 0.647099 4:-65424846 MsG0480021873.01.T01:CDS
GTTCTTATGTCTAACCTCGT+AGG 0.647799 4:-65424398 MsG0480021873.01.T01:CDS
AATTCCAAACAAACTCAAGA+GGG 0.648821 4:+65424764 None:intergenic
GTTGAGCATTACCACTGCTG+TGG 0.649328 4:+65424915 None:intergenic
GATGAGATTAAGAGGACCTG+AGG 0.656997 4:-65424671 MsG0480021873.01.T01:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATTTTTATATATTAAAAAA+GGG - Chr4:65424063-65424082 MsG0480021873.01.T01:intron 0.0%
!! TTAAGAATTAATCAAAAAAA+AGG - Chr4:65423108-65423127 MsG0480021873.01.T01:intron 10.0%
!!! AAATAAAACATCAAAATTTT+AGG + Chr4:65422762-65422781 None:intergenic 10.0%
!!! ATTAATGTTCTCTATTTTAT+TGG - Chr4:65422902-65422921 MsG0480021873.01.T01:intron 15.0%
!!! TTAATGTTCTCTATTTTATT+GGG - Chr4:65422903-65422922 MsG0480021873.01.T01:intron 15.0%
!! AAAATAATCTACAACACAAT+TGG - Chr4:65422947-65422966 MsG0480021873.01.T01:intron 20.0%
!! AAATAATCTACAACACAATT+GGG - Chr4:65422948-65422967 MsG0480021873.01.T01:intron 20.0%
!! TGAGTAAAGAAAAGAAAATT+AGG + Chr4:65423415-65423434 None:intergenic 20.0%
!! TTATTATCAAGAAAATGTTG+AGG + Chr4:65423463-65423482 None:intergenic 20.0%
!!! TGAATCATAGTTTTAAAAAC+TGG + Chr4:65424658-65424677 None:intergenic 20.0%
!!! TTTAGAATGATAAATGAGTT+TGG - Chr4:65423487-65423506 MsG0480021873.01.T01:intron 20.0%
! AAATCAACCATAAATCAAAG+AGG + Chr4:65422882-65422901 None:intergenic 25.0%
! AGTGGTTATTTATTGTGAAT+TGG - Chr4:65423540-65423559 MsG0480021873.01.T01:intron 25.0%
! CATCACATACATATATATAG+TGG - Chr4:65423522-65423541 MsG0480021873.01.T01:intron 25.0%
! CGGCAAAAAACAAATTTAAT+TGG + Chr4:65423140-65423159 None:intergenic 25.0%
! GATGTAATAGTGTGTAATTA+AGG - Chr4:65423170-65423189 MsG0480021873.01.T01:intron 25.0%
! GCATATATAATCACAACATT+TGG + Chr4:65424217-65424236 None:intergenic 25.0%
! GCTCACAATTGTATATTTAT+TGG - Chr4:65424686-65424705 MsG0480021873.01.T01:CDS 25.0%
! GTATCAAGAAAATTTCAAGA+CGG + Chr4:65423215-65423234 None:intergenic 25.0%
! GTGGTTATTTATTGTGAATT+GGG - Chr4:65423541-65423560 MsG0480021873.01.T01:intron 25.0%
! TACTCTTATTCTTCTTAGTT+TGG - Chr4:65422854-65422873 MsG0480021873.01.T01:intron 25.0%
! TCAAACATGAAAAAACAGAA+GGG + Chr4:65423262-65423281 None:intergenic 25.0%
! TTCAAACATGAAAAAACAGA+AGG + Chr4:65423263-65423282 None:intergenic 25.0%
! TTCAATAAAAAACATTGACC+CGG + Chr4:65424035-65424054 None:intergenic 25.0%
!!! GGTATTGACAATAAATTGTA+CGG - Chr4:65423562-65423581 MsG0480021873.01.T01:intron 25.0%
!!! GTTTTTTCATGTTTGAATTG+AGG - Chr4:65423266-65423285 MsG0480021873.01.T01:intron 25.0%
AATCGCTATGAAAACCAAAA+AGG - Chr4:65424875-65424894 MsG0480021873.01.T01:CDS 30.0%
AATTCCAAACAAACTCAAGA+GGG + Chr4:65422617-65422636 None:intergenic 30.0%
ACCGATAGAAAAAATGACTT+TGG - Chr4:65423003-65423022 MsG0480021873.01.T01:intron 30.0%
AGAGATAAATATACGCACAT+AGG - Chr4:65423815-65423834 MsG0480021873.01.T01:intron 30.0%
ATAAAAAGTAACGACGAGTT+GGG - Chr4:65423622-65423641 MsG0480021873.01.T01:intron 30.0%
ATACACTTCCATATCTACTT+GGG - Chr4:65424742-65424761 MsG0480021873.01.T01:CDS 30.0%
ATATGTATGTGATGTGTAGT+GGG + Chr4:65423516-65423535 None:intergenic 30.0%
ATCGCTATGAAAACCAAAAA+GGG - Chr4:65424876-65424895 MsG0480021873.01.T01:CDS 30.0%
ATGCTCAACACAAGAAATAT+TGG - Chr4:65422477-65422496 MsG0480021873.01.T01:CDS 30.0%
GTGAAATACAATCCATTTAC+CGG + Chr4:65423037-65423056 None:intergenic 30.0%
TATATGTATGTGATGTGTAG+TGG + Chr4:65423517-65423536 None:intergenic 30.0%
TATCATATTCAAAGCAGCTA+TGG - Chr4:65424947-65424966 MsG0480021873.01.T01:CDS 30.0%
! ATATGGAAGTGTATCATGTA+TGG + Chr4:65424736-65424755 None:intergenic 30.0%
! CTATTACATCCCTTGATTTT+CGG + Chr4:65423160-65423179 None:intergenic 30.0%
! TCTTTCTCTTGATTAGTTCA+CGG + Chr4:65422641-65422660 None:intergenic 30.0%
! TTGTAGTAAACAAGTTGACT+CGG + Chr4:65424518-65424537 None:intergenic 30.0%
! TTTTATTGGGTTTGTGTTTC+AGG - Chr4:65422916-65422935 MsG0480021873.01.T01:intron 30.0%
!!! CATAGTTTTAAAAACTGGAC+CGG + Chr4:65424653-65424672 None:intergenic 30.0%
!!! GCCAAAGTCATTTTTTCTAT+CGG + Chr4:65423007-65423026 None:intergenic 30.0%
!!! TGTTTTTTGCCGAAAATCAA+GGG - Chr4:65423148-65423167 MsG0480021873.01.T01:intron 30.0%
!!! TTGTTTTTTGCCGAAAATCA+AGG - Chr4:65423147-65423166 MsG0480021873.01.T01:intron 30.0%
AATACAATCCATTTACCGGA+TGG + Chr4:65423033-65423052 None:intergenic 35.0%
ATACCTAAAGTAAGCCAATC+AGG - Chr4:65422582-65422601 MsG0480021873.01.T01:intron 35.0%
CTCAACACAAGAAATATTGG+AGG - Chr4:65422480-65422499 MsG0480021873.01.T01:CDS 35.0%
GAATTCCAAACAAACTCAAG+AGG + Chr4:65422618-65422637 None:intergenic 35.0%
GATAAAAAGTAACGACGAGT+TGG - Chr4:65423621-65423640 MsG0480021873.01.T01:intron 35.0%
GATACACTTCCATATCTACT+TGG - Chr4:65424741-65424760 MsG0480021873.01.T01:CDS 35.0%
GATTTGGAGATGAGATTAAG+AGG - Chr4:65422699-65422718 MsG0480021873.01.T01:intron 35.0%
TAAATAGCAATGCATACCTC+AGG + Chr4:65423073-65423092 None:intergenic 35.0%
TCGCTATGAAAACCAAAAAG+GGG - Chr4:65424877-65424896 MsG0480021873.01.T01:CDS 35.0%
TTATCCAACCCAAGTAGATA+TGG + Chr4:65424753-65424772 None:intergenic 35.0%
TTTGCATTTCCTTCACTGAT+TGG + Chr4:65422509-65422528 None:intergenic 35.0%
! CTTTAACTACCTCTTTTGTG+TGG - Chr4:65424805-65424824 MsG0480021873.01.T01:CDS 35.0%
! CTTTCTCTTGATTAGTTCAC+GGG + Chr4:65422640-65422659 None:intergenic 35.0%
! TTATCTTGTAGCTTTGCTCA+TGG - Chr4:65422677-65422696 MsG0480021873.01.T01:intron 35.0%
! TTCATCTATGAACCCCTTTT+TGG + Chr4:65424892-65424911 None:intergenic 35.0%
! TTTGGAGCCTCTTTGATTTA+TGG - Chr4:65422872-65422891 MsG0480021873.01.T01:intron 35.0%
!! ATTCCTGATTGGCTTACTTT+AGG + Chr4:65422588-65422607 None:intergenic 35.0%
!! GATTGGCTTACTTTAGGTAT+TGG + Chr4:65422582-65422601 None:intergenic 35.0%
!! TTCGTTTGTCAAGTTGGATT+TGG - Chr4:65423931-65423950 MsG0480021873.01.T01:intron 35.0%
!! TTGTCAAGTTGGATTTGGAT+CGG - Chr4:65423936-65423955 MsG0480021873.01.T01:intron 35.0%
AATGACTTTGGCAAACCATC+CGG - Chr4:65423015-65423034 MsG0480021873.01.T01:intron 40.0%
ACAACTACGCAAATGCTTAG+TGG + Chr4:65424154-65424173 None:intergenic 40.0%
ACTTCCATATCTACTTGGGT+TGG - Chr4:65424746-65424765 MsG0480021873.01.T01:CDS 40.0%
AGTCATCCGATTCTTTCATG+CGG - Chr4:65424562-65424581 MsG0480021873.01.T01:intron 40.0%
ATCCAACTTGACAAACGAAC+CGG + Chr4:65423930-65423949 None:intergenic 40.0%
ATGCAAAAACCAGAGTGCAA+AGG - Chr4:65422522-65422541 MsG0480021873.01.T01:CDS 40.0%
ATTAATGTGTCACACACCTC+AGG + Chr4:65422726-65422745 None:intergenic 40.0%
CATGAAAGAATCGGATGACT+CGG + Chr4:65424562-65424581 None:intergenic 40.0%
GATCGGTCATGTTATTGAAC+CGG - Chr4:65423953-65423972 MsG0480021873.01.T01:intron 40.0%
GTATTGGGTCATTAGGTCAT+TGG + Chr4:65424613-65424632 None:intergenic 40.0%
GTTCTTATGTCTAACCTCGT+AGG - Chr4:65422980-65422999 MsG0480021873.01.T01:intron 40.0%
TAGGTGCATGATTTGATCGA+CGG + Chr4:65423396-65423415 None:intergenic 40.0%
TGGTTGAACCACTGAATCAT+TGG + Chr4:65424593-65424612 None:intergenic 40.0%
! TGTAGCTTTGCTCATGGATT+TGG - Chr4:65422683-65422702 MsG0480021873.01.T01:intron 40.0%
!! GATGACTCGGTCATTTCATT+CGG + Chr4:65424549-65424568 None:intergenic 40.0%
!!! CATAGTTTTGAAACCCGAAC+CGG - Chr4:65423862-65423881 MsG0480021873.01.T01:intron 40.0%
!!! TCAATTCGCCGGTTTTTAAC+CGG + Chr4:65424004-65424023 None:intergenic 40.0%
!!! TTTTTTCTATCGGTCCTACG+AGG + Chr4:65422997-65423016 None:intergenic 40.0%
AAAACCGGCGAATTGATGAG+CGG - Chr4:65424008-65424027 MsG0480021873.01.T01:intron 45.0%
AAACAAGCCCTACCATGGAT+CGG - Chr4:65424087-65424106 MsG0480021873.01.T01:intron 45.0%
AACCAGAGTGCAAAGGTCTT+TGG - Chr4:65422529-65422548 MsG0480021873.01.T01:CDS 45.0%
AAGAGCAAATTCCACAGCAG+TGG - Chr4:65422452-65422471 MsG0480021873.01.T01:CDS 45.0%
ACCAGAGTGCAAAGGTCTTT+GGG - Chr4:65422530-65422549 MsG0480021873.01.T01:CDS 45.0%
ATCCTGCAACCACACAAAAG+AGG + Chr4:65424817-65424836 None:intergenic 45.0%
CCAAACAAACTCAAGAGGGT+TGG + Chr4:65422613-65422632 None:intergenic 45.0%
GATGAGATTAAGAGGACCTG+AGG - Chr4:65422707-65422726 MsG0480021873.01.T01:intron 45.0%
GGATTGTATTTCACTGCGAC+AGG - Chr4:65423043-65423062 MsG0480021873.01.T01:intron 45.0%
GTCGAACCGCATGAAAGAAT+CGG + Chr4:65424571-65424590 None:intergenic 45.0%
TGGAGGTTACCAATCAGTGA+AGG - Chr4:65422497-65422516 MsG0480021873.01.T01:CDS 45.0%
TTGGCAAACCATCCGGTAAA+TGG - Chr4:65423022-65423041 MsG0480021873.01.T01:intron 45.0%
TTGTATTTCACTGCGACAGG+TGG - Chr4:65423046-65423065 MsG0480021873.01.T01:intron 45.0%
! CCAACCCTCTTGAGTTTGTT+TGG - Chr4:65422610-65422629 MsG0480021873.01.T01:intron 45.0%
! TACCTCTTTTGTGTGGTTGC+AGG - Chr4:65424812-65424831 MsG0480021873.01.T01:CDS 45.0%
!! AACCGGTTCGTTTGTCAAGT+TGG - Chr4:65423925-65423944 MsG0480021873.01.T01:intron 45.0%
!!! CTATTTTTATATATTAAAAA+AGG - Chr4:65424062-65424081 MsG0480021873.01.T01:intron 5.0%
AAACGAACCGGTTACCCTTC+CGG + Chr4:65423918-65423937 None:intergenic 50.0%
AACAAGCCCTACCATGGATC+GGG - Chr4:65424088-65424107 MsG0480021873.01.T01:intron 50.0%
AGGGAAAACAAGCCCTACCA+TGG - Chr4:65424082-65424101 MsG0480021873.01.T01:intron 50.0%
CAGAGTGCAAAGGTCTTTGG+GGG - Chr4:65422532-65422551 MsG0480021873.01.T01:CDS 50.0%
CCAGAGTGCAAAGGTCTTTG+GGG - Chr4:65422531-65422550 MsG0480021873.01.T01:CDS 50.0%
CGGTCAAGCCGGTTAAAAAC+CGG - Chr4:65423993-65424012 MsG0480021873.01.T01:intron 50.0%
CGGTTCGACCAATGATTCAG+TGG - Chr4:65424582-65424601 MsG0480021873.01.T01:intron 50.0%
GAGGGTTGGAGATTCCTGAT+TGG + Chr4:65422599-65422618 None:intergenic 50.0%
GTTATTGAACCGGTGCGAAC+CGG - Chr4:65423963-65423982 MsG0480021873.01.T01:intron 50.0%
GTTGAGCATTACCACTGCTG+TGG + Chr4:65422466-65422485 None:intergenic 50.0%
TTCGATCCCGATCCATGGTA+GGG + Chr4:65424097-65424116 None:intergenic 50.0%
!! ATCGACTCGGTGAGGGTATT+GGG + Chr4:65424628-65424647 None:intergenic 50.0%
!! GGTGAGGGTATTGGGTCATT+AGG + Chr4:65424620-65424639 None:intergenic 50.0%
!!! TTTTGAAACCCGAACCGGAC+CGG - Chr4:65423867-65423886 MsG0480021873.01.T01:intron 50.0%
ACCATGGATCGGGATCGAAC+AGG - Chr4:65424098-65424117 MsG0480021873.01.T01:intron 55.0%
CAATACCCTCACCGAGTCGA+TGG - Chr4:65424627-65424646 MsG0480021873.01.T01:intron 55.0%
CCCCAAAGACCTTTGCACTC+TGG + Chr4:65422534-65422553 None:intergenic 55.0%
GTTCGATCCCGATCCATGGT+AGG + Chr4:65424098-65424117 None:intergenic 55.0%
TCCTGTTCGATCCCGATCCA+TGG + Chr4:65424102-65424121 None:intergenic 55.0%
TTACCCTTCCGGTTCACGGT+CGG + Chr4:65423907-65423926 None:intergenic 55.0%
!! CATCGACTCGGTGAGGGTAT+TGG + Chr4:65424629-65424648 None:intergenic 55.0%
CCGGTTACCCTTCCGGTTCA+CGG + Chr4:65423911-65423930 None:intergenic 60.0%
CCGTGAACCGGAAGGGTAAC+CGG - Chr4:65423908-65423927 MsG0480021873.01.T01:intron 60.0%
CGGCGAATTGATGAGCGGAC+CGG - Chr4:65424013-65424032 MsG0480021873.01.T01:intron 60.0%
CGGTCCGCTCATCAATTCGC+CGG + Chr4:65424015-65424034 None:intergenic 60.0%
GGCGAATTGATGAGCGGACC+GGG - Chr4:65424014-65424033 MsG0480021873.01.T01:intron 60.0%
! AACTGGACCGGCCATCGACT+CGG + Chr4:65424641-65424660 None:intergenic 60.0%
ACCCTCACCGAGTCGATGGC+CGG - Chr4:65424631-65424650 MsG0480021873.01.T01:intron 65.0%
CACTGCGACAGGTGGACCTG+AGG - Chr4:65423054-65423073 MsG0480021873.01.T01:intron 65.0%
CCGGCGTGAATCGGTCAAGC+CGG - Chr4:65423982-65424001 MsG0480021873.01.T01:intron 65.0%
CGGTGCGAACCGGCGTGAAT+CGG - Chr4:65423973-65423992 MsG0480021873.01.T01:intron 65.0%
CTTCCGGTTCACGGTCGGAC+CGG + Chr4:65423902-65423921 None:intergenic 65.0%
GGTCCGACCGTGAACCGGAA+GGG - Chr4:65423901-65423920 MsG0480021873.01.T01:intron 65.0%
! CCGGCTTGACCGATTCACGC+CGG + Chr4:65423985-65424004 None:intergenic 65.0%
!! ACCGGCCATCGACTCGGTGA+GGG + Chr4:65424635-65424654 None:intergenic 65.0%
!! CGATTCACGCCGGTTCGCAC+CGG + Chr4:65423975-65423994 None:intergenic 65.0%
CACGGTCGGACCGGTCCAAC+CGG + Chr4:65423893-65423912 None:intergenic 70.0%
CGGTCCGACCGTGAACCGGA+AGG - Chr4:65423900-65423919 MsG0480021873.01.T01:intron 70.0%
GAAACCCGAACCGGACCGGC+CGG - Chr4:65423871-65423890 MsG0480021873.01.T01:intron 70.0%
GGACCGGTCCGACCGTGAAC+CGG - Chr4:65423896-65423915 MsG0480021873.01.T01:intron 70.0%
TCCAACCGGCCGGTCCGGTT+CGG + Chr4:65423879-65423898 None:intergenic 70.0%
!! GACCGGCCATCGACTCGGTG+AGG + Chr4:65424636-65424655 None:intergenic 70.0%
ACCGGACCGGCCGGTTGGAC+CGG - Chr4:65423880-65423899 MsG0480021873.01.T01:intron 75.0%
ACCGGTCCAACCGGCCGGTC+CGG + Chr4:65423884-65423903 None:intergenic 75.0%
CCAACCGGCCGGTCCGGTTC+GGG + Chr4:65423878-65423897 None:intergenic 75.0%
GTCGGACCGGTCCAACCGGC+CGG + Chr4:65423889-65423908 None:intergenic 75.0%
! CCCGAACCGGACCGGCCGGT+TGG - Chr4:65423875-65423894 MsG0480021873.01.T01:intron 80.0%
Chromosome Type Strat End Strand Name
Chr4 gene 65422426 65424974 65422426 ID=MsG0480021873.01;Name=MsG0480021873.01
Chr4 mRNA 65422426 65424974 65422426 ID=MsG0480021873.01.T01;Parent=MsG0480021873.01;Name=MsG0480021873.01.T01;_AED=0.49;_eAED=0.49;_QI=0|0|0|1|1|0.66|3|0|193
Chr4 exon 65424672 65424974 65424672 ID=MsG0480021873.01.T01:exon:18079;Parent=MsG0480021873.01.T01
Chr4 exon 65424325 65424462 65424325 ID=MsG0480021873.01.T01:exon:18078;Parent=MsG0480021873.01.T01
Chr4 exon 65422426 65422566 65422426 ID=MsG0480021873.01.T01:exon:18077;Parent=MsG0480021873.01.T01
Chr4 CDS 65424672 65424974 65424672 ID=MsG0480021873.01.T01:cds;Parent=MsG0480021873.01.T01
Chr4 CDS 65424325 65424462 65424325 ID=MsG0480021873.01.T01:cds;Parent=MsG0480021873.01.T01
Chr4 CDS 65422426 65422566 65422426 ID=MsG0480021873.01.T01:cds;Parent=MsG0480021873.01.T01
Gene Sequence

>MsG0480021873.01.T01

ATGGAAGAGAGGAATGAGAAAACAAGAAGAGCAAATTCCACAGCAGTGGTAATGCTCAACACAAGAAATATTGGAGGTTACCAATCAGTGAAGGAAATGCAAAAACCAGAGTGCAAAGGTCTTTGGGGGAATCAAATTTCTTTCTTACAAATACCAATACCTAAAGTAAGCCAATCAGGAATCTCCAACCCTCTTGAGTTTGTTTGGAATTCCCGTGAACTAATCAAGAGAAAGAGACGTTCTTTCAGTGTTTATCTTGTAGCTTTGCTCATGGATTTGGAGATGAGATTAAGAGGACCTGAGGCAGTAGCTAAAATAATCTACAACACAATTGGGAACTCAAGTGTTCTTATGTCTAACCTCGTAGGACCGATAGAAAAAATGACTTTGGCAAACCATCCGGTAAATGGATTGTATTTCACTGCGACAGGTGGACCTGAGGATTTAACAGTTACAATTATTAGCTACGAGAAAATATTAAGAATCGCTATGAAAACCAAAAAGGGGTTCATAGATGAACGTAAGTTGAAGTTCTGCATTGAGAAAGCAGCTGATATCATATTCAAAGCAGCTATGGAGTGA
Protein sequence

>MsG0480021873.01.T01

MEERNEKTRRANSTAVVMLNTRNIGGYQSVKEMQKPECKGLWGNQISFLQIPIPKVSQSGISNPLEFVWNSRELIKRKRRSFSVYLVALLMDLEMRLRGPEAVAKIIYNTIGNSSVLMSNLVGPIEKMTLANHPVNGLYFTATGGPEDLTVTIISYEKILRIAMKTKKGFIDERKLKFCIEKAADIIFKAAME*