AlfalfaGEDB | MsG0480022030.01

M. sativa cultivar ZhongmuNo.1 / MsG0480022030.01

KEGG: K17756
GO: GO:0016614 GO:0046577 GO:0050660 GO:0055114
PFAM: PF00732 PF05199

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480022030.01.T01	XP_003607656.2	94.562	754	34	3	1	747	1	754	0	1414

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480022030.01.T01	B5WWZ8	78.667	750	149	5	1	743	1	746	0	1206

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480022030.01.T01	G7JDV8	94.562	754	34	3	1	747	1	754	0.0	1414

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180001922.01	MsG0480022030.01	0.803762	2.986312e-49	1.513253e-46
MsG0180003379.01	MsG0480022030.01	0.811049	8.503543e-51	5.210178e-48
MsG0280006935.01	MsG0480022030.01	0.801877	7.317250e-49	3.533703e-46
MsG0280007011.01	MsG0480022030.01	0.805939	1.047639e-49	5.614588e-47
MsG0280007109.01	MsG0480022030.01	0.809524	1.813124e-50	1.066979e-47
MsG0480018321.01	MsG0480022030.01	0.800823	1.203003e-48	5.656318e-46
MsG0480020144.01	MsG0480022030.01	0.812857	3.432192e-51	2.206345e-48
MsG0480022030.01	MsG0580024956.01	0.814999	1.157400e-51	7.884622e-49
MsG0480022030.01	MsG0580025278.01	0.819260	1.275060e-52	9.763424e-50
MsG0480022030.01	MsG0780036633.01	0.810742	9.906613e-51	6.020444e-48

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PPI

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Gene1	Gene2	Type
MsG0580025277.01	MsG0480022030.01	PPI
MsG0880045379.01	MsG0480022030.01	PPI
MsG0880045474.01	MsG0480022030.01	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	bit score
MsG0480022030.01.T01	MTR_4g080890	94.562	754	34	3	1	747	1	754	1414
MsG0480022030.01.T01	MTR_4g080823	90.704	753	63	3	1	746	10	762	1368
MsG0480022030.01.T01	MTR_0651s0010	93.262	653	37	3	1	646	1	653	1201
MsG0480022030.01.T01	MTR_7g097210	56.662	743	306	10	4	741	3	734	831
MsG0480022030.01.T01	MTR_1g075650	61.684	582	173	8	161	741	3	535	716
MsG0480022030.01.T01	MTR_7g097210	59.756	574	219	7	169	741	2	564	672
MsG0480022030.01.T01	MTR_5g025650	43.295	783	378	15	12	745	64	829	588
MsG0480022030.01.T01	MTR_8g072210	42.397	776	376	15	19	745	57	810	565

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	bit score
MsG0480022030.01.T01	AT3G23410	58.639	735	286	4	22	744	15	743	863
MsG0480022030.01.T01	AT4G28570	56.376	745	311	5	2	741	7	742	833
MsG0480022030.01.T01	AT4G28570	56.376	745	311	5	2	741	7	742	833
MsG0480022030.01.T01	AT3G23410	61.004	677	255	3	71	744	5	675	833
MsG0480022030.01.T01	AT1G03990	50.331	755	350	11	7	746	13	757	742
MsG0480022030.01.T01	AT4G19380	42.838	754	386	15	18	745	34	768	555
MsG0480022030.01.T01	AT4G19380	43.087	745	379	15	27	745	1	726	551

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Find 150 sgRNAs with CRISPR-Local

Find 243 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATCAAGTATTAAGAAAA+GGG	0.465765	4:+67185131	MsG0480022030.01.T01:CDS
AAAATGGTGATAATCCAGCA+TGG	0.647627	4:+67184531	MsG0480022030.01.T01:CDS
AAACCGCTAGAAATCATCAA+TGG	0.561746	4:-67185481	None:intergenic
AAAGAGAAAAGATTGTGCAG+AGG	0.669869	4:+67184307	MsG0480022030.01.T01:CDS
AAAGGTGCTGTGATAATAAC+AGG	0.471872	4:+67185292	MsG0480022030.01.T01:CDS
AAAGTAACAATTTCAGCAGG+TGG	0.598692	4:+67185442	MsG0480022030.01.T01:CDS
AAAGTGGTTGTTCTAGAGAA+AGG	0.509206	4:+67184809	MsG0480022030.01.T01:CDS
AAATGTGATGCGGTAATTGT+TGG	0.502713	4:+67184734	MsG0480022030.01.T01:CDS
AACAAGGGACTCAAGTTACA+TGG	0.588903	4:+67185251	MsG0480022030.01.T01:CDS
AACAGAAAGAAGAAATGTTT+GGG	0.405430	4:+67185364	MsG0480022030.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ACAATAATAAGAGACAAAGC+AGG	+	Chr4:67186212-67186231	MsG0480022030.01.T01:CDS	30.0%
ACCAACAATATGAAAATGGA+GGG	+	Chr4:67184882-67184901	MsG0480022030.01.T01:CDS	30.0%
ATATTCAGCACTATATCCAT+GGG	+	Chr4:67186130-67186149	MsG0480022030.01.T01:CDS	30.0%
ATGGACCAACAATATGAAAA+TGG	+	Chr4:67184878-67184897	MsG0480022030.01.T01:CDS	30.0%
ATGTCCACTTTCTTAAGTAT+TGG	-	Chr4:67184497-67184516	None:intergenic	30.0%
CAACAATCTCTATCTAACAA+AGG	+	Chr4:67184668-67184687	MsG0480022030.01.T01:CDS	30.0%
CAACAGAAAGAAGAAATGTT+TGG	+	Chr4:67185363-67185382	MsG0480022030.01.T01:CDS	30.0%
CGATTGAATTCAATTTGTAG+CGG	-	Chr4:67186265-67186284	None:intergenic	30.0%
GAAGATTATTCATCACTAGA+AGG	+	Chr4:67184848-67184867	MsG0480022030.01.T01:CDS	30.0%
GAATCAAAATCAGATCTCAA+AGG	+	Chr4:67185571-67185590	MsG0480022030.01.T01:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr4	gene	67183045	67186709	67183045	ID=MsG0480022030.01;Name=MsG0480022030.01
Chr4	mRNA	67183045	67186709	67183045	ID=MsG0480022030.01.T01;Parent=MsG0480022030.01;Name=MsG0480022030.01.T01;_AED=0.37;_eAED=0.37;_QI=0\|0\|0\|1\|1\|1\|4\|0\|747
Chr4	exon	67183045	67183248	67183045	ID=MsG0480022030.01.T01:exon:8150;Parent=MsG0480022030.01.T01
Chr4	exon	67184141	67184413	67184141	ID=MsG0480022030.01.T01:exon:8151;Parent=MsG0480022030.01.T01
Chr4	exon	67184524	67185592	67184524	ID=MsG0480022030.01.T01:exon:8152;Parent=MsG0480022030.01.T01
Chr4	exon	67186012	67186709	67186012	ID=MsG0480022030.01.T01:exon:8153;Parent=MsG0480022030.01.T01
Chr4	CDS	67183045	67183248	67183045	ID=MsG0480022030.01.T01:cds;Parent=MsG0480022030.01.T01
Chr4	CDS	67184141	67184413	67184141	ID=MsG0480022030.01.T01:cds;Parent=MsG0480022030.01.T01
Chr4	CDS	67184524	67185592	67184524	ID=MsG0480022030.01.T01:cds;Parent=MsG0480022030.01.T01
Chr4	CDS	67186012	67186709	67186012	ID=MsG0480022030.01.T01:cds;Parent=MsG0480022030.01.T01

Gene Sequence

>MsG0480022030.01.T01

ATGAGAAGGGAGTGTCATCCATTGTTGAGGGGTGTGAGAGAGAATCGCAAATACAAACATGGATTTTCTGTGGCTGAGATGAAATCGCTCACAAGCATATGTGAGGTTATGTTTCCTTCACTGCCAATGGATGATGATGAATCAAGCAAGGATGTGCAGTCTTTCTTCAACATCTCTGCTTCCAAATATCCAATCCCTGACGAGGTTGCTGAGATGTTAGCAAAGAGGGCTGTAATAGAAGCAGTAATATTGATTAGAGTGATTTTATGGCTTCTAGCAACGAGGTTGGGCACTTTCTTGATTTGTGGTTTGCTATGTCTCAGTAAGAAATGGCCCTTTATCAGCAACTTCTCAAGCTTGTCTTTGGATAAAAGAGAAAAGATTGTGCAGAGGGCTCTCAAGCATAAATTCTTCACTCCTTTTAGAATTGCATTCGTTTACATCAAAATCCTATGCCTCTTTGTTTTCTTCTCTTGGGTTGATGAAAATGGTGATAATCCAGCATGGAAAGCCATTGGATATGAAGTATCAACAGCTGATGAGAAAATGACCAATGACACCAAAAAGAGACCCCTTGAAAAAGGAATTATAGAAACCATGCATGAACATGACACAACTCTTCAACAATCTCTATCTAACAAAGGCCTCAATGTCACACTAGACTCCAAAAGCAACATCCTCAAAATCAAATGTGATGCGGTAATTGTTGGTTCTGGCTGTGGAGGAGGTGTTGCAGCTTCTGTCCTATCAAAAGCTGGCTACAAAGTGGTTGTTCTAGAGAAAGGAAACTATTTTGTTCCCGAAGATTATTCATCACTAGAAGGTCCTTCAATGGACCAACAATATGAAAATGGAGGGATCCTAGCTTCTATTGATTCAAGAATACTTATTTTGGCAGGTTCAACAGTAGGTGGTGGTTCAGCTGTTAATTGGTCAGCTTGCATTAAAACACCAGAAAAGGTGTTGAAAGAATGGTCAGAGGAACACAAACTTTCACTATTTGAAAGCATAGAGTATCTATCTGCTATGGAAACTGTGTGTGAGAGGATTGGTGTCAATGAAAATTGTACACAAGAAGGATTCCAAAATCAAGTATTAAGAAAAGGGTGTCAAAATCTTGGCTTAAAAGTTGATTATGTGCCAAGAAATTCACCAGGGAATCATTATTGCGGTTCATGTGGTTATGGTTGTCCAAAAGGAGAGAAACAAGGGACTCAAGTTACATGGCTTGTAGATGCTGTTGAAAAAGGTGCTGTGATAATAACAGGATGCAAAGCTGAGAGGTTTTTGTTTGAAAATAGCTACAAGAATGGAAACAACAGAAAGAAGAAATGTTTGGGAGTTTTGGCAAAGACTTTAAGTAGTGGAGTCACAATGAATCTACAATTTGAAGCAAAAGTAACAATTTCAGCAGGTGGAGCACTTCTAACACCTCCATTGATGATTTCTAGCGGTTTAAAGAATAAAAACATTGGTAGAAACCTTCATCTTCATCCCGTGCTAATGACATGGGGATACTTTCCAGAATCAAAATCAGATCTCAAAGGTAAAGCGAATGAGGGAGGAATAATCACATCTGTCCATAGAGTACCATCATCATCAAATGATGATTCAATATCCGATACAAGAGCAATAATTGAAACACCATTACTAGGACCATCATCATATTCAGCACTATATCCATGGGAATCAGGACTAGACTACAAACAAAGAATGCTAAATTATCCAAGAACAGCACACTTCATAACAATAATAAGAGACAAAGCAGGTGGAAAAGTAACAACAGAGGGAAGAATCCGCTACAAATTGAATTCAATCGACAAAGAGAATATGAGAGCTGGTATACAACAAGCACTGAGAATTCTAATAGCAGCAGGAGCAGTTGAAGTAGGAACACACAGAAGTGGTGGTCAGAGAATTAAGTGTAATGAGAACACTAGCGAAAAGGAAATCGAAGAGTTTATAGATAGTGTTTATCCAATGGAAGGAGCATTGTTACCAGGTGAGAATTGGAATTTGTATACTTCTGCTCATCAAATGGGGAGTTGTAGAATGGGAGTGAATGAAAAGGAAGGTGCTGTTGATGAAAATGGTGAGAGTTGGGAAGCTGAAGGGTTATTTGTTTGTGATGCTAGTGTGCTTCCAACTGCTGTTGGTGTTAACCCTATGATTACTATTCAATCAACTGCATTTTGTATTTCAAATAGGATTGTAGATTTTCTTGGAAAGGGTCGAGAACCATAA

Protein sequence

>MsG0480022030.01.T01

MRRECHPLLRGVRENRKYKHGFSVAEMKSLTSICEVMFPSLPMDDDESSKDVQSFFNISASKYPIPDEVAEMLAKRAVIEAVILIRVILWLLATRLGTFLICGLLCLSKKWPFISNFSSLSLDKREKIVQRALKHKFFTPFRIAFVYIKILCLFVFFSWVDENGDNPAWKAIGYEVSTADEKMTNDTKKRPLEKGIIETMHEHDTTLQQSLSNKGLNVTLDSKSNILKIKCDAVIVGSGCGGGVAASVLSKAGYKVVVLEKGNYFVPEDYSSLEGPSMDQQYENGGILASIDSRILILAGSTVGGGSAVNWSACIKTPEKVLKEWSEEHKLSLFESIEYLSAMETVCERIGVNENCTQEGFQNQVLRKGCQNLGLKVDYVPRNSPGNHYCGSCGYGCPKGEKQGTQVTWLVDAVEKGAVIITGCKAERFLFENSYKNGNNRKKKCLGVLAKTLSSGVTMNLQFEAKVTISAGGALLTPPLMISSGLKNKNIGRNLHLHPVLMTWGYFPESKSDLKGKANEGGIITSVHRVPSSSNDDSISDTRAIIETPLLGPSSYSALYPWESGLDYKQRMLNYPRTAHFITIIRDKAGGKVTTEGRIRYKLNSIDKENMRAGIQQALRILIAAGAVEVGTHRSGGQRIKCNENTSEKEIEEFIDSVYPMEGALLPGENWNLYTSAHQMGSCRMGVNEKEGAVDENGESWEAEGLFVCDASVLPTAVGVNPMITIQSTAFCISNRIVDFLGKGREP*