Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280006935.01.T01 | XP_003593822.2 | 98.864 | 88 | 1 | 0 | 2 | 89 | 169 | 256 | 1.67E-52 | 184 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080047825.01 | MsG0280006935.01 | 0.813000 | 3.194104e-51 | 2.061230e-48 |
| MsG0080047912.01 | MsG0280006935.01 | 0.800829 | 1.199605e-48 | 5.641313e-46 |
| MsG0080048058.01 | MsG0280006935.01 | 0.837512 | 4.977204e-57 | 6.480480e-54 |
| MsG0080048177.01 | MsG0280006935.01 | 0.849208 | 3.746523e-60 | 7.051192e-57 |
| MsG0080048178.01 | MsG0280006935.01 | 0.884867 | 1.327929e-71 | 8.908781e-68 |
| MsG0080048210.01 | MsG0280006935.01 | 0.844806 | 6.017188e-59 | 9.828331e-56 |
| MsG0080048602.01 | MsG0280006935.01 | 0.812422 | 4.272863e-51 | 2.715788e-48 |
| MsG0080048765.01 | MsG0280006935.01 | 0.817505 | 3.185717e-52 | 2.324287e-49 |
| MsG0080048809.01 | MsG0280006935.01 | -0.836314 | 1.006380e-56 | 1.263738e-53 |
| MsG0080048830.01 | MsG0280006935.01 | 0.846488 | 2.104664e-59 | 3.628684e-56 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280006935.01.T01 | MTR_2g018010 | 98.864 | 88 | 1 | 0 | 2 | 89 | 169 | 256 | 2.03e-56 | 184 |
Find 21 sgRNAs with CRISPR-Local
Find 23 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAACCTACAATACCGCGTCA+TGG | 0.632672 | 2:-8210566 | None:intergenic |
| AACTGAAATTGAGGCTTTGG+AGG | 0.435945 | 2:+8210480 | MsG0280006935.01.T01:CDS |
| AAGGGTTGGTGGCCATGACG+CGG | 0.659773 | 2:+8210554 | MsG0280006935.01.T01:CDS |
| AGTTTCTGTGCTTAAAGGGT+TGG | 0.449301 | 2:+8210540 | MsG0280006935.01.T01:CDS |
| ATATGGAGGATGATGCTCTT+AGG | 0.267598 | 2:+8210344 | MsG0280006935.01.T01:CDS |
| CCTACAGTTTCTGTGCTTAA+AGG | 0.158681 | 2:+8210535 | MsG0280006935.01.T01:CDS |
| CCTTTAAGCACAGAAACTGT+AGG | 0.597906 | 2:-8210535 | None:intergenic |
| CTACAGTTTCTGTGCTTAAA+GGG | 0.404783 | 2:+8210536 | MsG0280006935.01.T01:CDS |
| CTTCGCAATTCTTCCGAATC+AGG | 0.273597 | 2:-8210412 | None:intergenic |
| GAATGGGAGGATTCCTGATT+CGG | 0.461877 | 2:+8210399 | MsG0280006935.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TTCTTGATCAGTTTGAGAAT+GGG | + | Chr2:8210383-8210402 | MsG0280006935.01.T01:CDS | 30.0% | |
| TTGTTGTAGAACTGAAATTG+AGG | + | Chr2:8210471-8210490 | MsG0280006935.01.T01:CDS | 30.0% | |
| TTTCTTGATCAGTTTGAGAA+TGG | + | Chr2:8210382-8210401 | MsG0280006935.01.T01:CDS | 30.0% | |
| CTACAGTTTCTGTGCTTAAA+GGG | + | Chr2:8210536-8210555 | MsG0280006935.01.T01:CDS | 35.0% | |
| TAGAACTGAAATTGAGGCTT+TGG | + | Chr2:8210477-8210496 | MsG0280006935.01.T01:CDS | 35.0% | |
| AGTTTCTGTGCTTAAAGGGT+TGG | + | Chr2:8210540-8210559 | MsG0280006935.01.T01:CDS | 40.0% | |
| ATATGGAGGATGATGCTCTT+AGG | + | Chr2:8210344-8210363 | MsG0280006935.01.T01:CDS | 40.0% | |
| CCTACAGTTTCTGTGCTTAA+AGG | + | Chr2:8210535-8210554 | MsG0280006935.01.T01:CDS | 40.0% | |
| CCTTTAAGCACAGAAACTGT+AGG | - | Chr2:8210538-8210557 | None:intergenic | 40.0% | |
| GAGCAGATTGTTAACCATGA+GGG | + | Chr2:8210502-8210521 | MsG0280006935.01.T01:CDS | 40.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr2 | gene | 8210322 | 8210591 | 8210322 | ID=MsG0280006935.01;Name=MsG0280006935.01 |
| Chr2 | mRNA | 8210322 | 8210591 | 8210322 | ID=MsG0280006935.01.T01;Parent=MsG0280006935.01;Name=MsG0280006935.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|89 |
| Chr2 | exon | 8210322 | 8210591 | 8210322 | ID=MsG0280006935.01.T01:exon:2382;Parent=MsG0280006935.01.T01 |
| Chr2 | CDS | 8210322 | 8210591 | 8210322 | ID=MsG0280006935.01.T01:cds;Parent=MsG0280006935.01.T01 |
| Gene Sequence |
>MsG0280006935.01.T01 ATGAGGGCTAAGCTTTTTATTGATATGGAGGATGATGCTCTTAGGGTTCGTTTCTTTGAGTTTCTTGATCAGTTTGAGAATGGGAGGATTCCTGATTCGGAAGAATTGCGAAGTTTTTACGTTGATAAGTTGCAGATTATGAATGCTGCTTGTTGTAGAACTGAAATTGAGGCTTTGGAGGAGCAGATTGTTAACCATGAGGGTGATATTGAGCCTACAGTTTCTGTGCTTAAAGGGTTGGTGGCCATGACGCGGTATTGTAGGTTTTGA |
| Protein sequence |
>MsG0280006935.01.T01 MRAKLFIDMEDDALRVRFFEFLDQFENGRIPDSEELRSFYVDKLQIMNAACCRTEIEALEEQIVNHEGDIEPTVSVLKGLVAMTRYCRF* |
