AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280006935.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280006935.01.T01 MTR_2g018010 98.864 88 1 0 2 89 169 256 2.03e-56 184
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280006935.01.T01 AT1G29340 61.364 88 34 0 2 89 181 268 6.29e-34 122

Find 21 sgRNAs with CRISPR-Local

Find 23 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTACAGTTTCTGTGCTTAA+AGG 0.158681 2:+8210535 MsG0280006935.01.T01:CDS
ATATGGAGGATGATGCTCTT+AGG 0.267598 2:+8210344 MsG0280006935.01.T01:CDS
CTTCGCAATTCTTCCGAATC+AGG 0.273597 2:-8210412 None:intergenic
TAGAACTGAAATTGAGGCTT+TGG 0.324110 2:+8210477 MsG0280006935.01.T01:CDS
TGGCCATGACGCGGTATTGT+AGG 0.366029 2:+8210563 MsG0280006935.01.T01:CDS
TTTCTTGATCAGTTTGAGAA+TGG 0.402402 2:+8210382 MsG0280006935.01.T01:CDS
CTACAGTTTCTGTGCTTAAA+GGG 0.404783 2:+8210536 MsG0280006935.01.T01:CDS
TTCTGTGCTTAAAGGGTTGG+TGG 0.421580 2:+8210543 MsG0280006935.01.T01:CDS
AACTGAAATTGAGGCTTTGG+AGG 0.435945 2:+8210480 MsG0280006935.01.T01:CDS
AGTTTCTGTGCTTAAAGGGT+TGG 0.449301 2:+8210540 MsG0280006935.01.T01:CDS
TTCTTGATCAGTTTGAGAAT+GGG 0.456367 2:+8210383 MsG0280006935.01.T01:CDS
GAATGGGAGGATTCCTGATT+CGG 0.461877 2:+8210399 MsG0280006935.01.T01:CDS
TATGGAGGATGATGCTCTTA+GGG 0.462714 2:+8210345 MsG0280006935.01.T01:CDS
TTGATCAGTTTGAGAATGGG+AGG 0.564834 2:+8210386 MsG0280006935.01.T01:CDS
TTGTTGTAGAACTGAAATTG+AGG 0.586669 2:+8210471 MsG0280006935.01.T01:CDS
CCTTTAAGCACAGAAACTGT+AGG 0.597906 2:-8210535 None:intergenic
TAGGCTCAATATCACCCTCA+TGG 0.626253 2:-8210516 None:intergenic
AAACCTACAATACCGCGTCA+TGG 0.632672 2:-8210566 None:intergenic
AAGGGTTGGTGGCCATGACG+CGG 0.659773 2:+8210554 MsG0280006935.01.T01:CDS
GAGCAGATTGTTAACCATGA+GGG 0.670920 2:+8210502 MsG0280006935.01.T01:CDS
GGAGCAGATTGTTAACCATG+AGG 0.676840 2:+8210501 MsG0280006935.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAGCTTTTTATTGATATGG+AGG + Chr2:8210330-8210349 MsG0280006935.01.T01:CDS 25.0%
TTCTTGATCAGTTTGAGAAT+GGG + Chr2:8210383-8210402 MsG0280006935.01.T01:CDS 30.0%
TTGTTGTAGAACTGAAATTG+AGG + Chr2:8210471-8210490 MsG0280006935.01.T01:CDS 30.0%
TTTCTTGATCAGTTTGAGAA+TGG + Chr2:8210382-8210401 MsG0280006935.01.T01:CDS 30.0%
! GGCTAAGCTTTTTATTGATA+TGG + Chr2:8210327-8210346 MsG0280006935.01.T01:CDS 30.0%
CTACAGTTTCTGTGCTTAAA+GGG + Chr2:8210536-8210555 MsG0280006935.01.T01:CDS 35.0%
TAGAACTGAAATTGAGGCTT+TGG + Chr2:8210477-8210496 MsG0280006935.01.T01:CDS 35.0%
AGTTTCTGTGCTTAAAGGGT+TGG + Chr2:8210540-8210559 MsG0280006935.01.T01:CDS 40.0%
ATATGGAGGATGATGCTCTT+AGG + Chr2:8210344-8210363 MsG0280006935.01.T01:CDS 40.0%
CCTACAGTTTCTGTGCTTAA+AGG + Chr2:8210535-8210554 MsG0280006935.01.T01:CDS 40.0%
CCTTTAAGCACAGAAACTGT+AGG - Chr2:8210538-8210557 None:intergenic 40.0%
GAGCAGATTGTTAACCATGA+GGG + Chr2:8210502-8210521 MsG0280006935.01.T01:CDS 40.0%
TATGGAGGATGATGCTCTTA+GGG + Chr2:8210345-8210364 MsG0280006935.01.T01:CDS 40.0%
TTGATCAGTTTGAGAATGGG+AGG + Chr2:8210386-8210405 MsG0280006935.01.T01:CDS 40.0%
! AACTGAAATTGAGGCTTTGG+AGG + Chr2:8210480-8210499 MsG0280006935.01.T01:CDS 40.0%
AAACCTACAATACCGCGTCA+TGG - Chr2:8210569-8210588 None:intergenic 45.0%
CTTCGCAATTCTTCCGAATC+AGG - Chr2:8210415-8210434 None:intergenic 45.0%
GAATGGGAGGATTCCTGATT+CGG + Chr2:8210399-8210418 MsG0280006935.01.T01:CDS 45.0%
GGAGCAGATTGTTAACCATG+AGG + Chr2:8210501-8210520 MsG0280006935.01.T01:CDS 45.0%
TAGGCTCAATATCACCCTCA+TGG - Chr2:8210519-8210538 None:intergenic 45.0%
TTCTGTGCTTAAAGGGTTGG+TGG + Chr2:8210543-8210562 MsG0280006935.01.T01:CDS 45.0%
TGGCCATGACGCGGTATTGT+AGG + Chr2:8210563-8210582 MsG0280006935.01.T01:CDS 55.0%
!! AAGGGTTGGTGGCCATGACG+CGG + Chr2:8210554-8210573 MsG0280006935.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr2 gene 8210322 8210591 8210322 ID=MsG0280006935.01;Name=MsG0280006935.01
Chr2 mRNA 8210322 8210591 8210322 ID=MsG0280006935.01.T01;Parent=MsG0280006935.01;Name=MsG0280006935.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|89
Chr2 exon 8210322 8210591 8210322 ID=MsG0280006935.01.T01:exon:2382;Parent=MsG0280006935.01.T01
Chr2 CDS 8210322 8210591 8210322 ID=MsG0280006935.01.T01:cds;Parent=MsG0280006935.01.T01
Gene Sequence

>MsG0280006935.01.T01

ATGAGGGCTAAGCTTTTTATTGATATGGAGGATGATGCTCTTAGGGTTCGTTTCTTTGAGTTTCTTGATCAGTTTGAGAATGGGAGGATTCCTGATTCGGAAGAATTGCGAAGTTTTTACGTTGATAAGTTGCAGATTATGAATGCTGCTTGTTGTAGAACTGAAATTGAGGCTTTGGAGGAGCAGATTGTTAACCATGAGGGTGATATTGAGCCTACAGTTTCTGTGCTTAAAGGGTTGGTGGCCATGACGCGGTATTGTAGGTTTTGA

Protein sequence

>MsG0280006935.01.T01

MRAKLFIDMEDDALRVRFFEFLDQFENGRIPDSEELRSFYVDKLQIMNAACCRTEIEALEEQIVNHEGDIEPTVSVLKGLVAMTRYCRF*