AlfalfaGEDB | MsG0480022462.01

M. sativa cultivar ZhongmuNo.1 / MsG0480022462.01

KEGG: K03857
GO: GO:0006506
PFAM: PF00534 PF08288

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480022462.01.T01	XP_013457081.1	91.083	471	19	4	1	468	1	451	0	863

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480022462.01.T01	Q94BX4	77.042	453	83	2	7	459	3	434	0	698

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480022462.01.T01	A0A072UN35	91.083	471	19	4	1	468	1	451	0.0	863

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
MsG0480022462.01	MsG0380012860.01	PPI
MsG0580029371.01	MsG0480022462.01	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480022462.01.T01	MTR_4g089065	91.083	471	19	4	1	468	1	451	0.0	863
MsG0480022462.01.T01	MTR_2g095220	81.007	437	64	3	6	442	4	421	0.0	721
MsG0480022462.01.T01	MTR_2g095220	81.618	408	56	3	6	413	4	392	0.0	672
MsG0480022462.01.T01	MTR_2g095220	80.240	334	47	3	6	339	4	318	0.0	534
MsG0480022462.01.T01	MTR_2g047140	90.000	70	7	0	44	113	140	209	6.59e-36	132

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480022462.01.T01	AT3G45100	77.042	453	83	2	7	459	3	434	0.0	698
MsG0480022462.01.T01	AT3G45100	77.042	453	83	2	7	459	3	434	0.0	698
MsG0480022462.01.T01	AT3G45100	77.042	453	83	2	7	459	3	434	0.0	698
MsG0480022462.01.T01	AT1G73160	26.542	373	216	13	25	369	98	440	9.50e-17	83.2

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Find 110 sgRNAs with CRISPR-Local

Find 271 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACAAGCCAACTTACATT+AGG	0.434799	4:-72847286	None:intergenic
AAAACCATTCAAGGAATGAT+CGG	0.584527	4:-72846939	None:intergenic
AACACATTTCCAACTTTGTA+TGG	0.315745	4:+72846785	MsG0480022462.01.T01:CDS
AACCTTCTCCGGCGCCAAAC+CGG	0.405406	4:-72847071	None:intergenic
AAGGTTCGTTTCATTATTGG+AGG	0.502256	4:+72847807	MsG0480022462.01.T01:intron
AATGACTTCAACAAGCAAGT+CGG	0.671637	4:-72847242	None:intergenic
AATGTGTTCTGATTAAAGAA+TGG	0.616415	4:-72846770	None:intergenic
ACAACTAGCAGCCTCTAATA+TGG	0.367430	4:-72849066	None:intergenic
ACAATTTCCGATCCGCTAGG+AGG	0.612287	4:-72847181	None:intergenic
ACACCTCTCTTGCGGAGCAT+GGG	0.525914	4:+72849917	MsG0480022462.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAACAAGCCAACTTACATT+AGG	-	Chr4:72847289-72847308	None:intergenic	30.0%
AAAACCATTCAAGGAATGAT+CGG	-	Chr4:72846942-72846961	None:intergenic	30.0%
AACACATTTCCAACTTTGTA+TGG	+	Chr4:72846785-72846804	MsG0480022462.01.T01:CDS	30.0%
AACTTATGTATCCCATTTGA+AGG	+	Chr4:72847788-72847807	MsG0480022462.01.T01:intron	30.0%
AATGAAACGAACCTTCAAAT+GGG	-	Chr4:72847802-72847821	None:intergenic	30.0%
ACAATTCAACAGTTATTACG+GGG	-	Chr4:72848742-72848761	None:intergenic	30.0%
AGCACGATCATATACAATTT+CGG	-	Chr4:72849536-72849555	None:intergenic	30.0%
AGTGGTTAAAGCAGTATATA+AGG	-	Chr4:72846388-72846407	None:intergenic	30.0%
AGTTGTAAAAGAAAATCCAC+TGG	+	Chr4:72849641-72849660	MsG0480022462.01.T01:intron	30.0%
ATGTGTTTCTCATACAAGTA+AGG	+	Chr4:72847030-72847049	MsG0480022462.01.T01:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr4	gene	72846195	72850203	72846195	ID=MsG0480022462.01;Name=MsG0480022462.01
Chr4	mRNA	72846195	72850203	72846195	ID=MsG0480022462.01.T01;Parent=MsG0480022462.01;Name=MsG0480022462.01.T01;_AED=0.39;_eAED=0.40;_QI=0\|0\|0\|1\|1\|1\|8\|0\|477
Chr4	exon	72846195	72846326	72846195	ID=MsG0480022462.01.T01:exon:31312;Parent=MsG0480022462.01.T01
Chr4	exon	72846683	72847318	72846683	ID=MsG0480022462.01.T01:exon:31313;Parent=MsG0480022462.01.T01
Chr4	exon	72847810	72847964	72847810	ID=MsG0480022462.01.T01:exon:31314;Parent=MsG0480022462.01.T01
Chr4	exon	72849040	72849130	72849040	ID=MsG0480022462.01.T01:exon:31315;Parent=MsG0480022462.01.T01
Chr4	exon	72849293	72849409	72849293	ID=MsG0480022462.01.T01:exon:31316;Parent=MsG0480022462.01.T01
Chr4	exon	72849493	72849596	72849493	ID=MsG0480022462.01.T01:exon:31317;Parent=MsG0480022462.01.T01
Chr4	exon	72849917	72850007	72849917	ID=MsG0480022462.01.T01:exon:31318;Parent=MsG0480022462.01.T01
Chr4	exon	72850096	72850203	72850096	ID=MsG0480022462.01.T01:exon:31319;Parent=MsG0480022462.01.T01
Chr4	CDS	72846195	72846326	72846195	ID=MsG0480022462.01.T01:cds;Parent=MsG0480022462.01.T01
Chr4	CDS	72846683	72847318	72846683	ID=MsG0480022462.01.T01:cds;Parent=MsG0480022462.01.T01
Chr4	CDS	72847810	72847964	72847810	ID=MsG0480022462.01.T01:cds;Parent=MsG0480022462.01.T01
Chr4	CDS	72849040	72849130	72849040	ID=MsG0480022462.01.T01:cds;Parent=MsG0480022462.01.T01
Chr4	CDS	72849293	72849409	72849293	ID=MsG0480022462.01.T01:cds;Parent=MsG0480022462.01.T01
Chr4	CDS	72849493	72849596	72849493	ID=MsG0480022462.01.T01:cds;Parent=MsG0480022462.01.T01
Chr4	CDS	72849917	72850007	72849917	ID=MsG0480022462.01.T01:cds;Parent=MsG0480022462.01.T01
Chr4	CDS	72850096	72850203	72850096	ID=MsG0480022462.01.T01:cds;Parent=MsG0480022462.01.T01

Gene Sequence

>MsG0480022462.01.T01

ATGGGTGATCGTGAAAATGAAAATGAAAAGCATTCGATTCTTATGGTCTCTGATTTTTTCTATCCCAACGTTGGTGGTGTCGAGAATCACATCTACTGCCTCTCTCAATGCTTGCTCAACTTAGGCCACAAGGTGGTGGTTGTAACTCATGCTTATGGAAAACGCTCTGGGGTCAGATACATGACAGGTGGTTTAAAAGTCTACTATGTTCCATGGAGACCATTCTTTAATCAGAACACATTTCCAACTTTGTATGGAACACTCCCAATTATAAGAACTATTCTCACTCGAGAAAGAATTACCGTTGTGCATGGACATCAAACCTTTTCAACACTTTGTCATGAGGCCCTTATGCATGCTAGAATCATGGGATACAAGGTTGTATTTACCGATCATTCCTTGAATGGTTTTAGTGATGCTGGAAGTATTCACATGAACAAGGTTTTGCAATTTACCTTGGCAGATGTGAGTCAGGCTATATGTGTTTCTCATACAAGTAAGGAGAACACGGTTTTGAGGTCCGGTTTGGCGCCGGAGAAGGTTTTTGTAATTCCTAATGCTGTTGACACTGCCAAGTTTAAGCCATTTAAGCCAGTGTCGGAGGCGGAGGCGGAGGCGGAGGAGGAGCGTCCTCCTAGCGGATCGGAAATTGTTATTGTTGTTATCAGTCGATTGGTTTACCGTAAAGGAGCCGACTTGCTTGTTGAAGTCATTCCAGAAGTGTGTCGATTGCATCCTAATGTAAGTTGGCTTGTTTTGCATAACGCGGTTCGTTTCATTATTGGAGGTGATGGACCTAAACGTGTACGGTTGGAGGAGATGAGAGAAAAGCATTCTCTTCAAGATCGAGTTGGTTTGTTGGGAGCTGTACAACATACAGATGTCCAGTCTGTTCTAATAAAAGGGCATATTTTCTTAAACAGTTCCTTAACAGAAGCTTTTTGTATAGCCATATTAGAGGCTGCTAGTTGTGGATTGTTAACAGTTAGCACACGCGTCGGAGGTGTTCCTGAGGTTGTACCAGATGATATGATTGTTTTGGCAGAACCTGATCCAAGTGATATGGTGCGCGCAATCCAGAAGGCGATAACTATGCTGCCTAAAATTGATCCTCAAGTCATGCACAATCGAATGAGGGAACTCTATAGCTGGCATGATGTTGCCAAAAGGACCGAAATTGTATATGATCGTGCTTTGAAGTGTTCTGATCAAAATCTACTTGAACGACTCTCACGACACCTCTCTTGCGGAGCATGGGCAGGAAAGATCTTTTGCTTGGTTATGATCTTCAGTTTTTTGTTATGGCGTATATTGGAGCTATGGCAGCCTGCTGATGATATTGAGGAGGCGCCGGATTTCATTCTCTCGCCCAACCGTGATGGATTTGGAGGGATGTTGCAGAAAACCCAACAATGCAGAAAATCAGAAGAATAG

Protein sequence

>MsG0480022462.01.T01

MGDRENENEKHSILMVSDFFYPNVGGVENHIYCLSQCLLNLGHKVVVVTHAYGKRSGVRYMTGGLKVYYVPWRPFFNQNTFPTLYGTLPIIRTILTRERITVVHGHQTFSTLCHEALMHARIMGYKVVFTDHSLNGFSDAGSIHMNKVLQFTLADVSQAICVSHTSKENTVLRSGLAPEKVFVIPNAVDTAKFKPFKPVSEAEAEAEEERPPSGSEIVIVVISRLVYRKGADLLVEVIPEVCRLHPNVSWLVLHNAVRFIIGGDGPKRVRLEEMREKHSLQDRVGLLGAVQHTDVQSVLIKGHIFLNSSLTEAFCIAILEAASCGLLTVSTRVGGVPEVVPDDMIVLAEPDPSDMVRAIQKAITMLPKIDPQVMHNRMRELYSWHDVAKRTEIVYDRALKCSDQNLLERLSRHLSCGAWAGKIFCLVMIFSFLLWRILELWQPADDIEEAPDFILSPNRDGFGGMLQKTQQCRKSEE*