AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580024489.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580024489.01.T01 MTR_5g012010 99.051 316 2 1 1 315 5 320 0.0 631
MsG0580024489.01.T01 MTR_2g012270 47.267 311 146 3 1 311 5 297 4.60e-103 304
MsG0580024489.01.T01 MTR_4g090900 49.828 291 129 4 1 291 5 278 1.82e-97 290
MsG0580024489.01.T01 MTR_1g056560 48.988 247 125 1 45 291 44 289 1.87e-84 258
MsG0580024489.01.T01 MTR_1g056550 45.769 260 140 1 32 291 27 285 2.68e-84 256
MsG0580024489.01.T01 MTR_7g106420 46.122 245 131 1 49 293 31 274 8.69e-78 239
MsG0580024489.01.T01 MTR_2g088700 45.238 294 147 4 21 312 23 304 9.57e-77 237
MsG0580024489.01.T01 MTR_5g020670 45.392 293 136 6 1 290 5 276 8.21e-74 229
MsG0580024489.01.T01 MTR_2g088700 46.154 273 133 4 21 291 23 283 3.16e-72 226
MsG0580024489.01.T01 MTR_6g028140 42.803 264 150 1 30 293 18 280 1.51e-71 224
MsG0580024489.01.T01 MTR_8g073900 34.659 176 79 4 1 174 5 146 8.08e-20 84.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580024489.01.T01 AT1G08560 64.873 316 100 5 1 315 5 310 1.62e-129 372
MsG0580024489.01.T01 AT1G61290 47.896 309 139 4 1 307 5 293 1.95e-100 297
MsG0580024489.01.T01 AT1G61290 47.896 309 139 4 1 307 5 293 1.95e-100 297
MsG0580024489.01.T01 AT1G11250 53.226 248 113 2 46 291 26 272 3.17e-91 274
MsG0580024489.01.T01 AT4G03330 47.267 311 149 3 1 311 5 300 3.34e-91 274
MsG0580024489.01.T01 AT3G11820 50.575 261 128 1 31 291 29 288 2.65e-88 268
MsG0580024489.01.T01 AT3G11820 52.632 247 116 1 45 291 12 257 5.28e-88 266
MsG0580024489.01.T01 AT3G52400 43.750 288 157 2 10 293 3 289 8.10e-79 244
MsG0580024489.01.T01 AT3G03800 43.019 265 150 1 49 313 38 301 5.28e-68 215
MsG0580024489.01.T01 AT3G03800 43.019 265 150 1 49 313 38 301 5.28e-68 215
MsG0580024489.01.T01 AT2G18260 37.942 311 182 4 1 311 5 304 1.56e-67 213
MsG0580024489.01.T01 AT2G18260 37.942 311 182 4 1 311 5 304 1.56e-67 213
MsG0580024489.01.T01 AT5G08080 50.228 219 108 1 73 291 61 278 1.23e-64 206
MsG0580024489.01.T01 AT5G08080 50.228 219 108 1 73 291 61 278 1.23e-64 206
MsG0580024489.01.T01 AT5G08080 50.228 219 108 1 73 291 72 289 1.53e-64 206
MsG0580024489.01.T01 AT5G08080 46.809 141 74 1 73 213 61 200 1.78e-29 112
MsG0580024489.01.T01 AT5G08080 46.809 141 74 1 73 213 61 200 1.78e-29 112

Find 46 sgRNAs with CRISPR-Local

Find 63 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGAGATTGAGAAGAGTTTAT+TGG 0.159342 5:-5509554 MsG0580024489.01.T01:CDS
GGAGATGATGAAAGTTTCTT+AGG 0.183110 5:-5509655 MsG0580024489.01.T01:CDS
GGAGCTTATGATGGAGTTTC+AGG 0.211805 5:-5510038 MsG0580024489.01.T01:intron
TGAAGAAAGCAAAGTGTATT+AGG 0.275539 5:-5510186 MsG0580024489.01.T01:CDS
AACTGATATGAATTTGTTCT+TGG 0.340603 5:-5510359 MsG0580024489.01.T01:CDS
ACTAGAATTGCTGTTACTAA+TGG 0.388743 5:-5510079 MsG0580024489.01.T01:CDS
ACATGGAAGAGGAAAGGTTC+TGG 0.392183 5:-5509617 MsG0580024489.01.T01:CDS
AAGATCTTCACTGACCCATT+TGG 0.394651 5:-5510383 MsG0580024489.01.T01:CDS
AATTCATATCAGTTTCCAAA+TGG 0.400693 5:+5510368 None:intergenic
TGAGAAAGTAAGAACAGAAA+TGG 0.403034 5:-5510329 MsG0580024489.01.T01:CDS
ATTCTAGTTCTATAAATTGC+TGG 0.404484 5:+5510094 None:intergenic
TAGGACTCAACTCGAAGAAA+TGG 0.407461 5:-5510167 MsG0580024489.01.T01:CDS
TGAAGCACAAGGTGAGAAAA+TGG 0.413624 5:-5509494 MsG0580024489.01.T01:CDS
GATGAAGTTGATTTAGAAGC+TGG 0.444831 5:-5510436 MsG0580024489.01.T01:CDS
GCAGGAAGTGGATGTGCATT+GGG 0.446829 5:-5509381 MsG0580024489.01.T01:CDS
AGAAGATATTATACAGTGAC+AGG 0.449572 5:-5509721 MsG0580024489.01.T01:CDS
CTGCTGAAACTCACTACAAT+GGG 0.453019 5:+5509319 None:intergenic
CTGAAGCTTTGAAAGAATTG+AGG 0.453110 5:-5510240 MsG0580024489.01.T01:CDS
GCTGCTAATAGAAGACTCTC+TGG 0.458709 5:-5510133 MsG0580024489.01.T01:CDS
ATTCATATCAGTTTCCAAAT+GGG 0.460811 5:+5510369 None:intergenic
GCTTCTCATTATGTTAAAGA+TGG 0.464968 5:-5509445 MsG0580024489.01.T01:CDS
TGAAGCTTTGAAAGAATTGA+GGG 0.466131 5:-5510239 MsG0580024489.01.T01:CDS
GAGATGATGAAAGTTTCTTA+GGG 0.469669 5:-5509654 MsG0580024489.01.T01:CDS
TATGAATTTGTTCTTGGAAG+AGG 0.470619 5:-5510353 MsG0580024489.01.T01:CDS
AGCAGGAAGTGGATGTGCAT+TGG 0.476019 5:-5509382 MsG0580024489.01.T01:CDS
ATTGAGAAGATAATATCAAA+TGG 0.477269 5:-5509676 MsG0580024489.01.T01:CDS
ATCAGAAAAGTAGCAGGAAG+TGG 0.480407 5:-5509393 MsG0580024489.01.T01:CDS
AACAGTATCAGAAAAGTAGC+AGG 0.504319 5:-5509399 MsG0580024489.01.T01:CDS
GAAACTCAAGGAGCTTATGA+TGG 0.521896 5:-5510047 MsG0580024489.01.T01:CDS
GATGGTACAAAGAATCTTCA+CGG 0.525784 5:-5509427 MsG0580024489.01.T01:CDS
GTATGTTACAACAGGGGCTG+AGG 0.531696 5:-5509780 MsG0580024489.01.T01:intron
GAGAAAGTAAGAACAGAAAT+GGG 0.536281 5:-5510328 MsG0580024489.01.T01:CDS
TGATGAAAGTTTCTTAGGGA+AGG 0.540453 5:-5509650 MsG0580024489.01.T01:CDS
AGACTCTCTGGACTTAAAGA+TGG 0.542560 5:-5510121 MsG0580024489.01.T01:CDS
GCAATACAAGAACATGGAAG+AGG 0.566682 5:-5509628 MsG0580024489.01.T01:CDS
ACTGCTGAAACTCACTACAA+TGG 0.567938 5:+5509318 None:intergenic
AGCGAATACAAAGAAACAGT+AGG 0.587219 5:-5509745 MsG0580024489.01.T01:CDS
GCGAATACAAAGAAACAGTA+GGG 0.593645 5:-5509744 MsG0580024489.01.T01:CDS
TGGCTTGCGTAAGAAACTCA+AGG 0.596215 5:-5510059 MsG0580024489.01.T01:CDS
AGGGGAACATGCTGATGAAG+AGG 0.599762 5:-5509701 MsG0580024489.01.T01:CDS
ACAAGAACATGGAAGAGGAA+AGG 0.606969 5:-5509623 MsG0580024489.01.T01:CDS
GGGAAGGCAATACAAGAACA+TGG 0.609551 5:-5509634 MsG0580024489.01.T01:CDS
AGGAAAGGTTCTGGAAACTG+TGG 0.660613 5:-5509608 MsG0580024489.01.T01:CDS
GAAGATATTATACAGTGACA+GGG 0.694420 5:-5509720 MsG0580024489.01.T01:CDS
GCTGTTATGGTTGAAGCACA+AGG 0.705464 5:-5509505 MsG0580024489.01.T01:CDS
AAGATATTATACAGTGACAG+GGG 0.720898 5:-5509719 MsG0580024489.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GAAATTAGTCTTATTTAATA+AGG + Chr5:5509916-5509935 None:intergenic 15.0%
!!! GAATTTATGTATCAATTTTA+AGG - Chr5:5509854-5509873 MsG0580024489.01.T01:intron 15.0%
!! ATTGAGAAGATAATATCAAA+TGG - Chr5:5510123-5510142 MsG0580024489.01.T01:CDS 20.0%
!!! TCATCAAATATTTTTGGATA+TGG - Chr5:5510272-5510291 MsG0580024489.01.T01:CDS 20.0%
!!! TTTTTTTAGATCAACATAGT+TGG + Chr5:5509331-5509350 None:intergenic 20.0%
! AATTCATATCAGTTTCCAAA+TGG + Chr5:5509434-5509453 None:intergenic 25.0%
! ATTCATATCAGTTTCCAAAT+GGG + Chr5:5509433-5509452 None:intergenic 25.0%
! TCAGATAATGTCATATTCTA+AGG - Chr5:5509934-5509953 MsG0580024489.01.T01:intron 25.0%
!! AACTGATATGAATTTGTTCT+TGG - Chr5:5509440-5509459 MsG0580024489.01.T01:CDS 25.0%
!! GATTATACTTTTGCTTATTC+TGG - Chr5:5510440-5510459 MsG0580024489.01.T01:CDS 25.0%
!! GGAACTTCATCAAATATTTT+TGG - Chr5:5510266-5510285 MsG0580024489.01.T01:CDS 25.0%
!!! ATTCTAGTTCTATAAATTGC+TGG + Chr5:5509708-5509727 None:intergenic 25.0%
AAACCTTGTATGTTACAACA+GGG - Chr5:5510012-5510031 MsG0580024489.01.T01:intron 30.0%
AAGATATTATACAGTGACAG+GGG - Chr5:5510080-5510099 MsG0580024489.01.T01:CDS 30.0%
AGAAGATATTATACAGTGAC+AGG - Chr5:5510078-5510097 MsG0580024489.01.T01:CDS 30.0%
AGAGATTGAGAAGAGTTTAT+TGG - Chr5:5510245-5510264 MsG0580024489.01.T01:CDS 30.0%
GAAGATATTATACAGTGACA+GGG - Chr5:5510079-5510098 MsG0580024489.01.T01:CDS 30.0%
GAGAAAGTAAGAACAGAAAT+GGG - Chr5:5509471-5509490 MsG0580024489.01.T01:CDS 30.0%
GAGATGATGAAAGTTTCTTA+GGG - Chr5:5510145-5510164 MsG0580024489.01.T01:CDS 30.0%
GCTTCTCATTATGTTAAAGA+TGG - Chr5:5510354-5510373 MsG0580024489.01.T01:CDS 30.0%
TGAAGAAAGCAAAGTGTATT+AGG - Chr5:5509613-5509632 MsG0580024489.01.T01:CDS 30.0%
TGAGAAAGTAAGAACAGAAA+TGG - Chr5:5509470-5509489 MsG0580024489.01.T01:CDS 30.0%
! ACTAGAATTGCTGTTACTAA+TGG - Chr5:5509720-5509739 MsG0580024489.01.T01:CDS 30.0%
! TATGAATTTGTTCTTGGAAG+AGG - Chr5:5509446-5509465 MsG0580024489.01.T01:CDS 30.0%
!! ATTTTTGGATATGGCTGTTA+TGG - Chr5:5510281-5510300 MsG0580024489.01.T01:CDS 30.0%
!! TGAAGCTTTGAAAGAATTGA+GGG - Chr5:5509560-5509579 MsG0580024489.01.T01:CDS 30.0%
!!! TCTTCTGCTTTTTTTCTTTC+TGG - Chr5:5509804-5509823 MsG0580024489.01.T01:intron 30.0%
AACAGTATCAGAAAAGTAGC+AGG - Chr5:5510400-5510419 MsG0580024489.01.T01:CDS 35.0%
AACCTTGTATGTTACAACAG+GGG - Chr5:5510013-5510032 MsG0580024489.01.T01:intron 35.0%
AAGCAGAAGAGAAATTGACA+AGG + Chr5:5509794-5509813 None:intergenic 35.0%
AGCAGAAGAGAAATTGACAA+GGG + Chr5:5509793-5509812 None:intergenic 35.0%
AGCGAATACAAAGAAACAGT+AGG - Chr5:5510054-5510073 MsG0580024489.01.T01:CDS 35.0%
GAAACCTTGTATGTTACAAC+AGG - Chr5:5510011-5510030 MsG0580024489.01.T01:intron 35.0%
GATGGTACAAAGAATCTTCA+CGG - Chr5:5510372-5510391 MsG0580024489.01.T01:CDS 35.0%
GCGAATACAAAGAAACAGTA+GGG - Chr5:5510055-5510074 MsG0580024489.01.T01:CDS 35.0%
GGAGATGATGAAAGTTTCTT+AGG - Chr5:5510144-5510163 MsG0580024489.01.T01:CDS 35.0%
TGATGAAAGTTTCTTAGGGA+AGG - Chr5:5510149-5510168 MsG0580024489.01.T01:CDS 35.0%
! GATGAAGTTGATTTAGAAGC+TGG - Chr5:5509363-5509382 MsG0580024489.01.T01:CDS 35.0%
!! CTGAAGCTTTGAAAGAATTG+AGG - Chr5:5509559-5509578 MsG0580024489.01.T01:CDS 35.0%
AAGATCTTCACTGACCCATT+TGG - Chr5:5509416-5509435 MsG0580024489.01.T01:CDS 40.0%
ACAAGAACATGGAAGAGGAA+AGG - Chr5:5510176-5510195 MsG0580024489.01.T01:CDS 40.0%
ACTGCTGAAACTCACTACAA+TGG + Chr5:5510484-5510503 None:intergenic 40.0%
AGACTCTCTGGACTTAAAGA+TGG - Chr5:5509678-5509697 MsG0580024489.01.T01:CDS 40.0%
ATCAGAAAAGTAGCAGGAAG+TGG - Chr5:5510406-5510425 MsG0580024489.01.T01:CDS 40.0%
CTGCTGAAACTCACTACAAT+GGG + Chr5:5510483-5510502 None:intergenic 40.0%
GAAACTCAAGGAGCTTATGA+TGG - Chr5:5509752-5509771 MsG0580024489.01.T01:CDS 40.0%
GCAATACAAGAACATGGAAG+AGG - Chr5:5510171-5510190 MsG0580024489.01.T01:CDS 40.0%
TAGGACTCAACTCGAAGAAA+TGG - Chr5:5509632-5509651 MsG0580024489.01.T01:CDS 40.0%
TGAAGCACAAGGTGAGAAAA+TGG - Chr5:5510305-5510324 MsG0580024489.01.T01:CDS 40.0%
!! GAAGCTGGTCTTCAAAAACA+AGG - Chr5:5509378-5509397 MsG0580024489.01.T01:CDS 40.0%
ACATGGAAGAGGAAAGGTTC+TGG - Chr5:5510182-5510201 MsG0580024489.01.T01:CDS 45.0%
AGCCCCTGTTGTAACATACA+AGG + Chr5:5510018-5510037 None:intergenic 45.0%
GCTGCTAATAGAAGACTCTC+TGG - Chr5:5509666-5509685 MsG0580024489.01.T01:CDS 45.0%
GCTGTTATGGTTGAAGCACA+AGG - Chr5:5510294-5510313 MsG0580024489.01.T01:CDS 45.0%
GGGAAGGCAATACAAGAACA+TGG - Chr5:5510165-5510184 MsG0580024489.01.T01:CDS 45.0%
! GGAGCTTATGATGGAGTTTC+AGG - Chr5:5509761-5509780 MsG0580024489.01.T01:CDS 45.0%
! TGGCTTGCGTAAGAAACTCA+AGG - Chr5:5509740-5509759 MsG0580024489.01.T01:CDS 45.0%
!! AGGAAAGGTTCTGGAAACTG+TGG - Chr5:5510191-5510210 MsG0580024489.01.T01:CDS 45.0%
!! GCTGGTCTTCAAAAACAAGG+TGG - Chr5:5509381-5509400 MsG0580024489.01.T01:CDS 45.0%
AGCAGGAAGTGGATGTGCAT+TGG - Chr5:5510417-5510436 MsG0580024489.01.T01:CDS 50.0%
AGGGGAACATGCTGATGAAG+AGG - Chr5:5510098-5510117 MsG0580024489.01.T01:CDS 50.0%
GCAGGAAGTGGATGTGCATT+GGG - Chr5:5510418-5510437 MsG0580024489.01.T01:CDS 50.0%
GTATGTTACAACAGGGGCTG+AGG - Chr5:5510019-5510038 MsG0580024489.01.T01:intron 50.0%
Chromosome Type Strat End Strand Name
Chr5 gene 5509312 5510509 5509312 ID=MsG0580024489.01;Name=MsG0580024489.01
Chr5 mRNA 5509312 5510509 5509312 ID=MsG0580024489.01.T01;Parent=MsG0580024489.01;Name=MsG0580024489.01.T01;_AED=0.43;_eAED=0.43;_QI=0|0|0|1|1|1|2|0|315
Chr5 exon 5510039 5510509 5510039 ID=MsG0580024489.01.T01:exon:19099;Parent=MsG0580024489.01.T01
Chr5 exon 5509312 5509788 5509312 ID=MsG0580024489.01.T01:exon:19098;Parent=MsG0580024489.01.T01
Chr5 CDS 5510039 5510509 5510039 ID=MsG0580024489.01.T01:cds;Parent=MsG0580024489.01.T01
Chr5 CDS 5509312 5509788 5509312 ID=MsG0580024489.01.T01:cds;Parent=MsG0580024489.01.T01
Gene Sequence

>MsG0580024489.01.T01

ATGACAAAATCATTCACCAACTATGTTGATCTAAAAAAAGCAGCTATGAAAGATGAAGTTGATTTAGAAGCTGGTCTTCAAAAACAAGGTGGTGTTGAATTAACAAGATCTTCACTGACCCATTTGGAAACTGATATGAATTTGTTCTTGGAAGAGGCTGAGAAAGTAAGAACAGAAATGGGTGAAATTCGCGATATACTAACAAAGCTTGAACAAGCAAATGAAGAGAGCAAGAGTCTTCACAAAACTGAAGCTTTGAAAGAATTGAGGGAAAGAATTAATACTGATATTGTAACAGTTTTGAAGAAAGCAAAGTGTATTAGGACTCAACTCGAAGAAATGGATCGTGCTAATGCTGCTAATAGAAGACTCTCTGGACTTAAAGATGGTTCACCAGCAATTTATAGAACTAGAATTGCTGTTACTAATGGCTTGCGTAAGAAACTCAAGGAGCTTATGATGGAGTTTCAGGGGCTGAGGCAGAAAATGATGAGCGAATACAAAGAAACAGTAGGGAGAAGATATTATACAGTGACAGGGGAACATGCTGATGAAGAGGTAATTGAGAAGATAATATCAAATGGAGATGATGAAAGTTTCTTAGGGAAGGCAATACAAGAACATGGAAGAGGAAAGGTTCTGGAAACTGTGGTTGAGATACAAGACAGACATGATGCTGCTAAAGAGATTGAGAAGAGTTTATTGGAACTTCATCAAATATTTTTGGATATGGCTGTTATGGTTGAAGCACAAGGTGAGAAAATGGATGATATTGAACATCATGTTCTTCATGCTTCTCATTATGTTAAAGATGGTACAAAGAATCTTCACGGTGCTAAACAGTATCAGAAAAGTAGCAGGAAGTGGATGTGCATTGGGATTATACTTTTGCTTATTCTGGTTCTCATCATTGTTATTCCCATTGTAGTGAGTTTCAGCAGTTCTTGA

Protein sequence

>MsG0580024489.01.T01

MTKSFTNYVDLKKAAMKDEVDLEAGLQKQGGVELTRSSLTHLETDMNLFLEEAEKVRTEMGEIRDILTKLEQANEESKSLHKTEALKELRERINTDIVTVLKKAKCIRTQLEEMDRANAANRRLSGLKDGSPAIYRTRIAVTNGLRKKLKELMMEFQGLRQKMMSEYKETVGRRYYTVTGEHADEEVIEKIISNGDDESFLGKAIQEHGRGKVLETVVEIQDRHDAAKEIEKSLLELHQIFLDMAVMVEAQGEKMDDIEHHVLHASHYVKDGTKNLHGAKQYQKSSRKWMCIGIILLLILVLIIVIPIVVSFSSS*