AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480022556.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480022556.01.T01 MTR_3g014200 58.955 134 13 2 42 175 16 107 8.06e-46 146
MsG0480022556.01.T01 MTR_4g092090 57.463 134 15 2 42 175 28 119 2.29e-44 143
MsG0480022556.01.T01 MTR_4g092090 57.463 134 15 2 42 175 16 107 2.93e-44 142
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 37 sgRNAs with CRISPR-Local

Find 278 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGATAACTGTCGCGGTAAA+TGG 0.205854 4:-74055514 MsG0480022556.01.T01:CDS
CAAAATGACTTTCCTCATTC+AGG 0.208346 4:+74053080 None:intergenic
TCCTACTTAACAAATGTTCT+TGG 0.241073 4:-74053241 MsG0480022556.01.T01:CDS
TACTGCCGCATTTGATAAAC+TGG 0.258041 4:+74055554 None:intergenic
ATCAAATGCGGCAGTATTAC+AGG 0.275072 4:-74055547 MsG0480022556.01.T01:CDS
CATTACTGAATTCCATACTA+TGG 0.287699 4:-74056832 MsG0480022556.01.T01:CDS
ACCAACCTCGACTTGAGATT+TGG 0.293709 4:+74055454 None:intergenic
CCAACCTCGACTTGAGATTT+GGG 0.322144 4:+74055455 None:intergenic
AAAGACAAGGATCCATAGTA+TGG 0.357731 4:+74056820 None:intergenic
CTCGACTTGAGATTTGGGTT+TGG 0.382849 4:+74055460 None:intergenic
GATATGAAGCTTCACAAAAC+TGG 0.407195 4:-74053130 MsG0480022556.01.T01:CDS
TTGAGCGAAAACAACTCTCT+TGG 0.427889 4:-74053289 MsG0480022556.01.T01:CDS
CCTCCTTCTGATACACTCCT+TGG 0.434600 4:+74056910 None:intergenic
TTGATTTCTCACGAGCTTCG+AGG 0.444756 4:+74053183 None:intergenic
TCCAAGAACATTTGTTAAGT+AGG 0.446325 4:+74053240 None:intergenic
CTTCGAGGACTTCCTGCACA+TGG 0.464782 4:+74053198 None:intergenic
ATGCGGCAGTATTACAGGCT+TGG 0.485797 4:-74055542 MsG0480022556.01.T01:CDS
TACACTCCTTGGACTAGCTT+CGG 0.510165 4:+74056921 None:intergenic
TAAGTAGGAGAACACTAGAA+TGG 0.544733 4:+74053255 None:intergenic
CTGGATATGCCGAAAAGACA+AGG 0.552746 4:+74056807 None:intergenic
GCTATATGTAACCCATGTGC+AGG 0.554451 4:-74053210 MsG0480022556.01.T01:CDS
GTCGCGGTAAATGGAAAGCA+TGG 0.579467 4:-74055505 MsG0480022556.01.T01:CDS
CATTTAGACAAGCCTGAATG+AGG 0.583066 4:-74053092 None:intergenic
GTTCGAAAATTCCAGATGTG+TGG 0.583866 4:+74053155 None:intergenic
CCCAATCTAAAATCAAAACC+TGG 0.586712 4:-74056876 MsG0480022556.01.T01:CDS
GCAAAAGCAGTCTTACGACC+AGG 0.593778 4:+74056858 None:intergenic
TTCGAGGACTTCCTGCACAT+GGG 0.606612 4:+74053199 None:intergenic
CCAAGGAGTGTATCAGAAGG+AGG 0.615600 4:-74056910 MsG0480022556.01.T01:CDS
AGGAGTGTATCAGAAGGAGG+AGG 0.617812 4:-74056907 MsG0480022556.01.T01:CDS
GGTTCTCTTGATAACTGTCG+CGG 0.618772 4:-74055521 MsG0480022556.01.T01:CDS
ACTGCCGCATTTGATAAACT+GGG 0.632430 4:+74055555 None:intergenic
AATTTACCGAAGCTAGTCCA+AGG 0.641341 4:-74056927 MsG0480022556.01.T01:CDS
CAGCCCCAGTTTATCAAATG+CGG 0.642365 4:-74055559 MsG0480022556.01.T01:CDS
AGTCCAAGGAGTGTATCAGA+AGG 0.645187 4:-74056913 MsG0480022556.01.T01:CDS
CAAACCCAAATCTCAAGTCG+AGG 0.664433 4:-74055459 MsG0480022556.01.T01:intron
CTGCCGCATTTGATAAACTG+GGG 0.687116 4:+74055556 None:intergenic
GTATAACAAAGTTCTGTACG+TGG 0.736673 4:+74056950 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATAATTAATAATATTTTTT+AGG + Chr4:74053597-74053616 None:intergenic 0.0%
!!! ATAATTAATAATATTTTTTA+GGG + Chr4:74053596-74053615 None:intergenic 0.0%
!!! TAAAAAATTATATTTTTTTA+GGG - Chr4:74053451-74053470 MsG0480022556.01.T01:intron 0.0%
!!! TTAAAAAATTATATTTTTTT+AGG - Chr4:74053450-74053469 MsG0480022556.01.T01:intron 0.0%
!! AACATATATATATAATTGTA+AGG + Chr4:74055993-74056012 None:intergenic 10.0%
!!! TATATTAATTTGTTTGATTA+CGG - Chr4:74056646-74056665 MsG0480022556.01.T01:intron 10.0%
!! ATATGTTCAAAATCTTTAAA+GGG - Chr4:74054649-74054668 MsG0480022556.01.T01:intron 15.0%
!! TATATGTTCAAAATCTTTAA+AGG - Chr4:74054648-74054667 MsG0480022556.01.T01:intron 15.0%
!! TTATAAACGAGATAAATTAT+GGG + Chr4:74053308-74053327 None:intergenic 15.0%
!! TTTATAAACGAGATAAATTA+TGG + Chr4:74053309-74053328 None:intergenic 15.0%
!!! ACTTTGATATTTTATTATAG+TGG - Chr4:74054046-74054065 MsG0480022556.01.T01:intron 15.0%
!!! TAATTTGAAATGTTTTATCT+GGG - Chr4:74053616-74053635 MsG0480022556.01.T01:intron 15.0%
!!! TTAATTTGAAATGTTTTATC+TGG - Chr4:74053615-74053634 MsG0480022556.01.T01:intron 15.0%
!! ATATATAAATTAGCCATTGA+TGG + Chr4:74054633-74054652 None:intergenic 20.0%
!! TATATAAATTAGCCATTGAT+GGG + Chr4:74054632-74054651 None:intergenic 20.0%
!! TATATATAATTGTAAGGTAC+AGG + Chr4:74055987-74056006 None:intergenic 20.0%
!!! CTTTAATTATGTTAGTTTTG+TGG + Chr4:74053542-74053561 None:intergenic 20.0%
!!! TATTATAGTGGTTTTAAACT+TGG - Chr4:74054058-74054077 MsG0480022556.01.T01:intron 20.0%
! AATAGAAATAAACCCGAATT+CGG - Chr4:74053355-74053374 MsG0480022556.01.T01:intron 25.0%
! ACACAAACTTTATCAATACT+GGG + Chr4:74054457-74054476 None:intergenic 25.0%
! ACGGTTATGTTAATTATTAG+TGG - Chr4:74056665-74056684 MsG0480022556.01.T01:intron 25.0%
! ACTAAAGACCAAATGAATAA+TGG + Chr4:74056402-74056421 None:intergenic 25.0%
! GAATTAGAAATGATGACATT+AGG - Chr4:74054964-74054983 MsG0480022556.01.T01:intron 25.0%
! GGGTATATTTCATTGTTAAA+AGG + Chr4:74055315-74055334 None:intergenic 25.0%
! GGTTAAATATTCTTTGAACT+TGG - Chr4:74054670-74054689 MsG0480022556.01.T01:intron 25.0%
! TAACTACAACAACAGAAAAT+CGG + Chr4:74054292-74054311 None:intergenic 25.0%
! TAAGGACAAATAGAACAAAA+AGG + Chr4:74054697-74054716 None:intergenic 25.0%
! TACACTTGAACTAATGTATT+TGG + Chr4:74054925-74054944 None:intergenic 25.0%
! TATTATCTTGGATGAAATGA+TGG + Chr4:74056461-74056480 None:intergenic 25.0%
! TCAACTGAAAATAAAAACTG+AGG - Chr4:74055343-74055362 MsG0480022556.01.T01:intron 25.0%
! TCTAGCAAGTATAGTTAAAA+TGG + Chr4:74053426-74053445 None:intergenic 25.0%
! TTCTATAGTGATTTATGACT+GGG - Chr4:74053833-74053852 MsG0480022556.01.T01:intron 25.0%
! TTGTTGAATAGAAATGACAA+CGG + Chr4:74054238-74054257 None:intergenic 25.0%
! TTGTTGATGCATAAATATGT+TGG + Chr4:74053280-74053299 None:intergenic 25.0%
!! AAAGACGATTAATAAGTTGA+AGG + Chr4:74053391-74053410 None:intergenic 25.0%
!! AAGACGATTAATAAGTTGAA+GGG + Chr4:74053390-74053409 None:intergenic 25.0%
!! AATGCATCTAAAACAGAAAA+TGG + Chr4:74053791-74053810 None:intergenic 25.0%
!! ATGCATCTAAAACAGAAAAT+GGG + Chr4:74053790-74053809 None:intergenic 25.0%
!! ATTTTCAGTTGAAAGATACA+GGG + Chr4:74055335-74055354 None:intergenic 25.0%
!! TATTTTCAGTTGAAAGATAC+AGG + Chr4:74055336-74055355 None:intergenic 25.0%
!! TTATATGTGGCTTTTAGATT+TGG + Chr4:74055729-74055748 None:intergenic 25.0%
!! TTTTTCCTTTTGAATACTCA+CGG + Chr4:74055612-74055631 None:intergenic 25.0%
!!! AACAAAGCCATATTTTTATG+GGG + Chr4:74054810-74054829 None:intergenic 25.0%
!!! CAACAAAGCCATATTTTTAT+GGG + Chr4:74054811-74054830 None:intergenic 25.0%
!!! TTACGAATTTTTTATCCATG+AGG + Chr4:74055519-74055538 None:intergenic 25.0%
AAACTGTACGAGAAACTATT+AGG - Chr4:74055120-74055139 MsG0480022556.01.T01:intron 30.0%
AACTGAAGATCAAATGACTT+TGG + Chr4:74054851-74054870 None:intergenic 30.0%
AAGTGTAATTTACTGTAGAC+GGG - Chr4:74054938-74054957 MsG0480022556.01.T01:intron 30.0%
ACACATACAGTCATTTCAAA+CGG - Chr4:74053950-74053969 MsG0480022556.01.T01:intron 30.0%
ACGCAAAAAAGTTATGTTTC+GGG + Chr4:74054416-74054435 None:intergenic 30.0%
AGAATCAAACTAAGACATGA+AGG + Chr4:74055651-74055670 None:intergenic 30.0%
AGCACTTATAGTAGAAAAGA+TGG - Chr4:74055001-74055020 MsG0480022556.01.T01:intron 30.0%
AGTCAACTTAAAAGAAGACT+CGG + Chr4:74055896-74055915 None:intergenic 30.0%
AGTGTGTTAGGTTTATCATA+AGG + Chr4:74054715-74054734 None:intergenic 30.0%
ATCAATATATTTCGATCGTG+TGG + Chr4:74056433-74056452 None:intergenic 30.0%
ATTGAATCCAATAAGCTGTT+GGG - Chr4:74054262-74054281 MsG0480022556.01.T01:intron 30.0%
CAAAACTGGCAAAAAATGTA+TGG - Chr4:74056932-74056951 MsG0480022556.01.T01:CDS 30.0%
CAAGTGTAATTTACTGTAGA+CGG - Chr4:74054937-74054956 MsG0480022556.01.T01:intron 30.0%
CAATTTATAGCATTTGCCTT+TGG + Chr4:74055793-74055812 None:intergenic 30.0%
CACACAAACTTTATCAATAC+TGG + Chr4:74054458-74054477 None:intergenic 30.0%
CATCTGAGGATATATTATCT+TGG + Chr4:74056473-74056492 None:intergenic 30.0%
CATTACTGAATTCCATACTA+TGG - Chr4:74053216-74053235 MsG0480022556.01.T01:CDS 30.0%
CCACAATCAGAGAAAATAAA+AGG - Chr4:74053916-74053935 MsG0480022556.01.T01:intron 30.0%
GACGCAAAAAAGTTATGTTT+CGG + Chr4:74054417-74054436 None:intergenic 30.0%
GGATCTATAGATAAATGAGT+TGG - Chr4:74055145-74055164 MsG0480022556.01.T01:intron 30.0%
GTTCTATAGTGATTTATGAC+TGG - Chr4:74053832-74053851 MsG0480022556.01.T01:intron 30.0%
TACAAAATACCCAAACATCA+GGG - Chr4:74055831-74055850 MsG0480022556.01.T01:intron 30.0%
TATTGAATCCAATAAGCTGT+TGG - Chr4:74054261-74054280 MsG0480022556.01.T01:intron 30.0%
TCCTACTTAACAAATGTTCT+TGG - Chr4:74056807-74056826 MsG0480022556.01.T01:CDS 30.0%
TTGTCACTCCATTATTCATT+TGG - Chr4:74056391-74056410 MsG0480022556.01.T01:intron 30.0%
TTGTGGACTTAAACTTAAGA+AGG + Chr4:74053525-74053544 None:intergenic 30.0%
TTTCTGAACTGAAAAGCAAA+AGG + Chr4:74055680-74055699 None:intergenic 30.0%
TTTCTGCTATGTTATATGCT+GGG - Chr4:74054180-74054199 MsG0480022556.01.T01:intron 30.0%
! ACTTTTACATAGCAACGATA+AGG + Chr4:74054105-74054124 None:intergenic 30.0%
! AGACGATTAATAAGTTGAAG+GGG + Chr4:74053389-74053408 None:intergenic 30.0%
! CTTTTACATAGCAACGATAA+GGG + Chr4:74054104-74054123 None:intergenic 30.0%
! GTTAACTGTGTTTCTGTATT+GGG - Chr4:74054132-74054151 MsG0480022556.01.T01:intron 30.0%
! TCCAAGAACATTTGTTAAGT+AGG + Chr4:74056811-74056830 None:intergenic 30.0%
! TGCATCTAAAACAGAAAATG+GGG + Chr4:74053789-74053808 None:intergenic 30.0%
! TGGTTTTTGATAGAACACTA+TGG - Chr4:74055177-74055196 MsG0480022556.01.T01:intron 30.0%
! TGTAGAAGATCTGAAACTAT+GGG + Chr4:74056263-74056282 None:intergenic 30.0%
! TGTTAACTGTGTTTCTGTAT+TGG - Chr4:74054131-74054150 MsG0480022556.01.T01:intron 30.0%
! TTGATTTTAGATTGGGTGAT+GGG + Chr4:74053168-74053187 None:intergenic 30.0%
! TTTTCTGCTATGTTATATGC+TGG - Chr4:74054179-74054198 MsG0480022556.01.T01:intron 30.0%
!! ATAGAAATGACAACGGATTA+AGG + Chr4:74054231-74054250 None:intergenic 30.0%
!! CCAACAAAGCCATATTTTTA+TGG + Chr4:74054812-74054831 None:intergenic 30.0%
!! CCATAAAAATATGGCTTTGT+TGG - Chr4:74054809-74054828 MsG0480022556.01.T01:intron 30.0%
!! TAGAAATGACAACGGATTAA+GGG + Chr4:74054230-74054249 None:intergenic 30.0%
!! TTTGATTTTAGATTGGGTGA+TGG + Chr4:74053169-74053188 None:intergenic 30.0%
!!! AAAGCTTGTTTTTAAGCATC+TGG + Chr4:74056196-74056215 None:intergenic 30.0%
!!! ACCAGGTTTTGATTTTAGAT+TGG + Chr4:74053176-74053195 None:intergenic 30.0%
!!! CCAGGTTTTGATTTTAGATT+GGG + Chr4:74053175-74053194 None:intergenic 30.0%
!!! CCTTTTATTTTCTCTGATTG+TGG + Chr4:74053919-74053938 None:intergenic 30.0%
!!! CTTCTTTTAAGTTGACTACA+CGG - Chr4:74055899-74055918 MsG0480022556.01.T01:intron 30.0%
!!! TTTTTTGCGTCTTTTACTAG+TGG - Chr4:74054426-74054445 MsG0480022556.01.T01:intron 30.0%
AAAGACAAGGATCCATAGTA+TGG + Chr4:74053231-74053250 None:intergenic 35.0%
AAATGCAGTGAGATTAAGAG+AGG + Chr4:74056156-74056175 None:intergenic 35.0%
AAGATGGTAGAAACTAGACT+TGG - Chr4:74055017-74055036 MsG0480022556.01.T01:intron 35.0%
AATCAAAAAAGCCACACATC+TGG - Chr4:74056882-74056901 MsG0480022556.01.T01:CDS 35.0%
ACTACAAGCTTGTGAAGTAA+AGG + Chr4:74056029-74056048 None:intergenic 35.0%
AGATGGTAGAAACTAGACTT+GGG - Chr4:74055018-74055037 MsG0480022556.01.T01:intron 35.0%
ATCCTGAATACTTCTCAAGT+TGG + Chr4:74056621-74056640 None:intergenic 35.0%
ATGAAATGGTTGCAAATGTG+GGG + Chr4:74056075-74056094 None:intergenic 35.0%
ATGACATTAGGTAGAGAGTT+GGG - Chr4:74054976-74054995 MsG0480022556.01.T01:intron 35.0%
CAACCATTTCATCACTTGTT+GGG - Chr4:74056083-74056102 MsG0480022556.01.T01:intron 35.0%
CCTCAAAACAAGTTAGTAAG+TGG + Chr4:74056340-74056359 None:intergenic 35.0%
CTACAAAATACCCAAACATC+AGG - Chr4:74055830-74055849 MsG0480022556.01.T01:intron 35.0%
GAACAGGATAGCAGAATAAA+TGG + Chr4:74056366-74056385 None:intergenic 35.0%
GAGAGGATGATGTTTATATG+TGG + Chr4:74055742-74055761 None:intergenic 35.0%
GATATGAAGCTTCACAAAAC+TGG - Chr4:74056918-74056937 MsG0480022556.01.T01:CDS 35.0%
GATGAAATGGTTGCAAATGT+GGG + Chr4:74056076-74056095 None:intergenic 35.0%
GGCAAATGCTATAAATTGCA+TGG - Chr4:74055795-74055814 MsG0480022556.01.T01:intron 35.0%
GTATAACAAAGTTCTGTACG+TGG + Chr4:74053101-74053120 None:intergenic 35.0%
GTATTGGACAAAATTCACTG+AGG + Chr4:74055429-74055448 None:intergenic 35.0%
TAAATCATAACACGTCTCAG+CGG + Chr4:74055286-74055305 None:intergenic 35.0%
TAAGTAGGAGAACACTAGAA+TGG + Chr4:74056796-74056815 None:intergenic 35.0%
TATCCCAACAAGTGATGAAA+TGG + Chr4:74056089-74056108 None:intergenic 35.0%
TCCTGAATACTTCTCAAGTT+GGG + Chr4:74056620-74056639 None:intergenic 35.0%
TGATAAACCTAACACACTCT+TGG - Chr4:74054717-74054736 MsG0480022556.01.T01:intron 35.0%
TGATGAAATGGTTGCAAATG+TGG + Chr4:74056077-74056096 None:intergenic 35.0%
TGTACGAGAAACTATTAGGA+AGG - Chr4:74055124-74055143 MsG0480022556.01.T01:intron 35.0%
TTAGATTTGGCATCAAGACT+TGG + Chr4:74055716-74055735 None:intergenic 35.0%
TTCTACAGAGACTGAATATC+AGG - Chr4:74056276-74056295 MsG0480022556.01.T01:intron 35.0%
TTCTCTGATTGTGGAAAGAT+GGG + Chr4:74053910-74053929 None:intergenic 35.0%
TTGTTGGCCCCATAAAAATA+TGG - Chr4:74054800-74054819 MsG0480022556.01.T01:intron 35.0%
TTTCTCTGATTGTGGAAAGA+TGG + Chr4:74053911-74053930 None:intergenic 35.0%
! AAACTAGACTTGGGTTGTTT+GGG - Chr4:74055027-74055046 MsG0480022556.01.T01:intron 35.0%
! AAAGTTATGTTTCGGGTGAA+TGG + Chr4:74054409-74054428 None:intergenic 35.0%
! AAATGAGTTGGAGAAAGACA+TGG - Chr4:74055157-74055176 MsG0480022556.01.T01:intron 35.0%
! AACAAATTGAGGGTCGATTT+GGG + Chr4:74053742-74053761 None:intergenic 35.0%
! ACTATGGATCCTTGTCTTTT+CGG - Chr4:74053232-74053251 MsG0480022556.01.T01:CDS 35.0%
! AGGTGTTTCTGTAGCAAAAT+AGG - Chr4:74055451-74055470 MsG0480022556.01.T01:intron 35.0%
! ATCTAAAACAGAAAATGGGG+AGG + Chr4:74053786-74053805 None:intergenic 35.0%
! ATGGGCTTTTCTTTATCTGT+AGG + Chr4:74056245-74056264 None:intergenic 35.0%
! ATGTTTGGGTATTTTGTAGC+TGG + Chr4:74055829-74055848 None:intergenic 35.0%
! CCCAATCTAAAATCAAAACC+TGG - Chr4:74053172-74053191 MsG0480022556.01.T01:CDS 35.0%
! CGATAATTTGCGTTTCATTG+TGG + Chr4:74054342-74054361 None:intergenic 35.0%
! CTGTAGAAGATCTGAAACTA+TGG + Chr4:74056264-74056283 None:intergenic 35.0%
! GACGATTAATAAGTTGAAGG+GGG + Chr4:74053388-74053407 None:intergenic 35.0%
! GTGAATTTTGTCCAATACAC+AGG - Chr4:74055431-74055450 MsG0480022556.01.T01:intron 35.0%
! GTGTTGCTGGTGAATTAATT+TGG - Chr4:74056309-74056328 MsG0480022556.01.T01:intron 35.0%
! GTTTCTCGTACAGTTTTTCT+AGG + Chr4:74055116-74055135 None:intergenic 35.0%
! TAAATTAGCCATTGATGGGT+AGG + Chr4:74054628-74054647 None:intergenic 35.0%
! TGTTTGGGTATTTTGTAGCT+GGG + Chr4:74055828-74055847 None:intergenic 35.0%
! TTCTGTATTGGGTTTTGCTA+CGG - Chr4:74054143-74054162 MsG0480022556.01.T01:intron 35.0%
!! GCTTTGTTTATAGGAGGAAA+GGG + Chr4:74055584-74055603 None:intergenic 35.0%
!!! CATTGATTTTGACAGTTCAG+TGG - Chr4:74055870-74055889 MsG0480022556.01.T01:intron 35.0%
!!! CCACTTACTAACTTGTTTTG+AGG - Chr4:74056337-74056356 MsG0480022556.01.T01:intron 35.0%
AAGGAAAAACAGTGCGAAGT+TGG - Chr4:74055623-74055642 MsG0480022556.01.T01:intron 40.0%
AATTTACCGAAGCTAGTCCA+AGG - Chr4:74053121-74053140 MsG0480022556.01.T01:CDS 40.0%
ACTGCCGCATTTGATAAACT+GGG + Chr4:74054496-74054515 None:intergenic 40.0%
AGTCAGATCACTAGAGTTAG+GGG - Chr4:74055086-74055105 MsG0480022556.01.T01:intron 40.0%
ATCAAATGCGGCAGTATTAC+AGG - Chr4:74054501-74054520 MsG0480022556.01.T01:intron 40.0%
ATCGATTGACCCTGATGTTT+GGG + Chr4:74055843-74055862 None:intergenic 40.0%
ATCTTCAGTTGTCATCTGCT+TGG - Chr4:74054861-74054880 MsG0480022556.01.T01:intron 40.0%
ATTGTAAGGTACAGGCAGTA+TGG + Chr4:74055979-74055998 None:intergenic 40.0%
ATTTCGATCGTGTGGAACAT+TGG + Chr4:74056425-74056444 None:intergenic 40.0%
CTACAGAAACACCTGTGTAT+TGG + Chr4:74055445-74055464 None:intergenic 40.0%
CTCGACTGAGAACAAATTGA+GGG + Chr4:74053752-74053771 None:intergenic 40.0%
CTTCTCCGTGAGTATTCAAA+AGG - Chr4:74055604-74055623 MsG0480022556.01.T01:intron 40.0%
GAACAAATTGAGGGTCGATT+TGG + Chr4:74053743-74053762 None:intergenic 40.0%
GAGAAGACTAGTAGATCAGA+TGG - Chr4:74055058-74055077 MsG0480022556.01.T01:intron 40.0%
GAGACTGAATATCAGGCTAT+TGG - Chr4:74056283-74056302 MsG0480022556.01.T01:intron 40.0%
GAGTCAGATCACTAGAGTTA+GGG - Chr4:74055085-74055104 MsG0480022556.01.T01:intron 40.0%
GATGACATTAGGTAGAGAGT+TGG - Chr4:74054975-74054994 MsG0480022556.01.T01:intron 40.0%
GCAACCATTTCATCACTTGT+TGG - Chr4:74056082-74056101 MsG0480022556.01.T01:intron 40.0%
GGATACTTAAACACATTGCC+AGG - Chr4:74056104-74056123 MsG0480022556.01.T01:intron 40.0%
GTGAATAAAATACCTCAGCC+TGG + Chr4:74056125-74056144 None:intergenic 40.0%
GTTCGAAAATTCCAGATGTG+TGG + Chr4:74056896-74056915 None:intergenic 40.0%
TAATGCTTGAGACTGAAGCA+CGG + Chr4:74055944-74055963 None:intergenic 40.0%
TACTGCCGCATTTGATAAAC+TGG + Chr4:74054497-74054516 None:intergenic 40.0%
TATAGCATTTGCCTTTGGTG+TGG + Chr4:74055788-74055807 None:intergenic 40.0%
TCCTATTTCCTACCCATCAA+TGG - Chr4:74054617-74054636 MsG0480022556.01.T01:intron 40.0%
TGACATTAGGTAGAGAGTTG+GGG - Chr4:74054977-74054996 MsG0480022556.01.T01:intron 40.0%
TGCAAATGTGGGGTACAAAT+TGG + Chr4:74056065-74056084 None:intergenic 40.0%
TGGAGTGACAATCTAAGAAC+AGG + Chr4:74056382-74056401 None:intergenic 40.0%
TGTAGTTATGTCGTCAGAGA+AGG - Chr4:74054304-74054323 MsG0480022556.01.T01:intron 40.0%
TTCTCAGCTTAGACAGTAAG+AGG + Chr4:74056581-74056600 None:intergenic 40.0%
TTGAGCGAAAACAACTCTCT+TGG - Chr4:74056759-74056778 MsG0480022556.01.T01:intron 40.0%
TTGTAAGGTACAGGCAGTAT+GGG + Chr4:74055978-74055997 None:intergenic 40.0%
! AAGTCTTATCCCACTAAGTG+GGG + Chr4:74055226-74055245 None:intergenic 40.0%
! ATAGCAACGATAAGGGTATG+AGG + Chr4:74054097-74054116 None:intergenic 40.0%
! ATGCCAAAAGGTGTATAGAG+AGG + Chr4:74055759-74055778 None:intergenic 40.0%
! CAAGTCTTATCCCACTAAGT+GGG + Chr4:74055227-74055246 None:intergenic 40.0%
! CATCCTCTCTATACACCTTT+TGG - Chr4:74055753-74055772 MsG0480022556.01.T01:intron 40.0%
! CTTTTCGGCATATCCAGAAA+AGG - Chr4:74053247-74053266 MsG0480022556.01.T01:CDS 40.0%
! CTTTTGAATACTCACGGAGA+AGG + Chr4:74055606-74055625 None:intergenic 40.0%
! GAAACTAGACTTGGGTTGTT+TGG - Chr4:74055026-74055045 MsG0480022556.01.T01:intron 40.0%
! TATCGATTGACCCTGATGTT+TGG + Chr4:74055844-74055863 None:intergenic 40.0%
! TGTTGGAGACAAACCTTTTC+TGG + Chr4:74053263-74053282 None:intergenic 40.0%
! TTGATAACTGTCGCGGTAAA+TGG - Chr4:74054534-74054553 MsG0480022556.01.T01:intron 40.0%
!! ACGGAGAAGGCTTTGTTTAT+AGG + Chr4:74055593-74055612 None:intergenic 40.0%
!! GAGAAGGCTTTGTTTATAGG+AGG + Chr4:74055590-74055609 None:intergenic 40.0%
!! GGACATGGAAATAGCTTTGT+TGG - Chr4:74054784-74054803 MsG0480022556.01.T01:intron 40.0%
!! GGCTTTGTTTATAGGAGGAA+AGG + Chr4:74055585-74055604 None:intergenic 40.0%
!! GGGTACAAATTGGCTTTAGA+TGG + Chr4:74056055-74056074 None:intergenic 40.0%
!! GTTTTTAAGCATCTGGTGTC+TGG + Chr4:74056189-74056208 None:intergenic 40.0%
!! TCCCAACTTGAGAAGTATTC+AGG - Chr4:74056616-74056635 MsG0480022556.01.T01:intron 40.0%
!! TGAGGTGATATACTGACTTG+AGG + Chr4:74055386-74055405 None:intergenic 40.0%
AAACTGAGGAGTCTCTCTCA+AGG - Chr4:74055357-74055376 MsG0480022556.01.T01:intron 45.0%
AAGAGCAGAAACTGACTAGC+TGG + Chr4:74056556-74056575 None:intergenic 45.0%
AGAATCACTCAGAGTGACAG+AGG - Chr4:74056513-74056532 MsG0480022556.01.T01:intron 45.0%
AGGGGGATTCAAACCGAATT+CGG + Chr4:74053371-74053390 None:intergenic 45.0%
CAAACCCAAATCTCAAGTCG+AGG - Chr4:74054589-74054608 MsG0480022556.01.T01:intron 45.0%
CAGCCCCAGTTTATCAAATG+CGG - Chr4:74054489-74054508 MsG0480022556.01.T01:intron 45.0%
CAGTATATCACCTCACTTCG+TGG - Chr4:74055391-74055410 MsG0480022556.01.T01:intron 45.0%
CCACTTAGTGGGATAAGACT+TGG - Chr4:74055225-74055244 MsG0480022556.01.T01:intron 45.0%
CCTCGACTGAGAACAAATTG+AGG + Chr4:74053753-74053772 None:intergenic 45.0%
CTGCCGCATTTGATAAACTG+GGG + Chr4:74054495-74054514 None:intergenic 45.0%
CTGGATATGCCGAAAAGACA+AGG + Chr4:74053244-74053263 None:intergenic 45.0%
GACCTATAGCTCACAAAGTC+AGG - Chr4:74054763-74054782 MsG0480022556.01.T01:intron 45.0%
GACTAGTAGATCAGATGGAG+AGG - Chr4:74055063-74055082 MsG0480022556.01.T01:intron 45.0%
GCTATATGTAACCCATGTGC+AGG - Chr4:74056838-74056857 MsG0480022556.01.T01:CDS 45.0%
GCTGGGACTCTCAATATCTT+TGG - Chr4:74054197-74054216 MsG0480022556.01.T01:intron 45.0%
GGAGTCAGATCACTAGAGTT+AGG - Chr4:74055084-74055103 MsG0480022556.01.T01:intron 45.0%
GTACATGATGTGCATCCTCA+TGG - Chr4:74055501-74055520 MsG0480022556.01.T01:CDS 45.0%
GTCCTGACTTTGTGAGCTAT+AGG + Chr4:74054768-74054787 None:intergenic 45.0%
GTGTGGCTAAAGATGCCAAA+AGG + Chr4:74055771-74055790 None:intergenic 45.0%
TAGCTCACAAAGTCAGGACA+TGG - Chr4:74054769-74054788 MsG0480022556.01.T01:intron 45.0%
TCGAACACTTTGATGCACTC+TGG - Chr4:74054366-74054385 MsG0480022556.01.T01:intron 45.0%
TGATGTTGGGACTTCATCTG+AGG + Chr4:74056487-74056506 None:intergenic 45.0%
TGATTCTGCCAGCTGATGTT+GGG + Chr4:74056500-74056519 None:intergenic 45.0%
TTAAACACATTGCCAGGCTG+AGG - Chr4:74056110-74056129 MsG0480022556.01.T01:intron 45.0%
TTGATTTCTCACGAGCTTCG+AGG + Chr4:74056868-74056887 None:intergenic 45.0%
TTTGGCATCAAGACTTGGCT+TGG + Chr4:74055711-74055730 None:intergenic 45.0%
! ACCAACCTCGACTTGAGATT+TGG + Chr4:74054597-74054616 None:intergenic 45.0%
! AGGCTATTGGTTAGTGTTGC+TGG - Chr4:74056296-74056315 MsG0480022556.01.T01:intron 45.0%
! AGTCCAAGGAGTGTATCAGA+AGG - Chr4:74053135-74053154 MsG0480022556.01.T01:CDS 45.0%
! CCAACCTCGACTTGAGATTT+GGG + Chr4:74054596-74054615 None:intergenic 45.0%
! CCAAGTCTTATCCCACTAAG+TGG + Chr4:74055228-74055247 None:intergenic 45.0%
! CCTCAATTTGTTCTCAGTCG+AGG - Chr4:74053750-74053769 MsG0480022556.01.T01:intron 45.0%
! CTCGACTTGAGATTTGGGTT+TGG + Chr4:74054591-74054610 None:intergenic 45.0%
! GCCATTGATGGGTAGGAAAT+AGG + Chr4:74054621-74054640 None:intergenic 45.0%
! GGTTCTCTTGATAACTGTCG+CGG - Chr4:74054527-74054546 MsG0480022556.01.T01:intron 45.0%
! TGAGAAGCCAAGAGTGTGTT+AGG + Chr4:74054727-74054746 None:intergenic 45.0%
! TGTATTGGGTTTTGCTACGG+TGG - Chr4:74054146-74054165 MsG0480022556.01.T01:intron 45.0%
! TTGGGTATTTTGTAGCTGGG+AGG + Chr4:74055825-74055844 None:intergenic 45.0%
!! TACACTCCTTGGACTAGCTT+CGG + Chr4:74053130-74053149 None:intergenic 45.0%
!!! AAAAAATTATATTTTTTTAG+GGG - Chr4:74053452-74053471 MsG0480022556.01.T01:intron 5.0%
!!! TAATTAATAATATTTTTTAG+GGG + Chr4:74053595-74053614 None:intergenic 5.0%
ATGCGGCAGTATTACAGGCT+TGG - Chr4:74054506-74054525 MsG0480022556.01.T01:intron 50.0%
ATGGCGTCGTTTGAGTCATG+TGG - Chr4:74055196-74055215 MsG0480022556.01.T01:intron 50.0%
CCCAAATCTCAAGTCGAGGT+TGG - Chr4:74054593-74054612 MsG0480022556.01.T01:intron 50.0%
CCTCCTTCTGATACACTCCT+TGG + Chr4:74053141-74053160 None:intergenic 50.0%
CGGTGATGAGGAGGATTTGA+CGG + Chr4:74053657-74053676 None:intergenic 50.0%
GATGAAGTCCCAACATCAGC+TGG - Chr4:74056489-74056508 MsG0480022556.01.T01:intron 50.0%
GATTTGGGAGCAACCGAAGA+CGG + Chr4:74053727-74053746 None:intergenic 50.0%
GCAAAAGCAGTCTTACGACC+AGG + Chr4:74053193-74053212 None:intergenic 50.0%
GGAGGAAAGGGACTGTTGAT+AGG + Chr4:74055572-74055591 None:intergenic 50.0%
GGCATCTTTAGCCACACCAA+AGG - Chr4:74055774-74055793 MsG0480022556.01.T01:intron 50.0%
GGGGGATTCAAACCGAATTC+GGG + Chr4:74053370-74053389 None:intergenic 50.0%
GTCGCGGTAAATGGAAAGCA+TGG - Chr4:74054543-74054562 MsG0480022556.01.T01:intron 50.0%
GTGATTCTGCCAGCTGATGT+TGG + Chr4:74056501-74056520 None:intergenic 50.0%
TTCGAGGACTTCCTGCACAT+GGG + Chr4:74056852-74056871 None:intergenic 50.0%
! ACTGACTAGCTGGAAGGAAG+AGG + Chr4:74056546-74056565 None:intergenic 50.0%
! AGGAGTGTATCAGAAGGAGG+AGG - Chr4:74053141-74053160 MsG0480022556.01.T01:CDS 50.0%
! CCAAGGAGTGTATCAGAAGG+AGG - Chr4:74053138-74053157 MsG0480022556.01.T01:CDS 50.0%
! GCAGAAACTGACTAGCTGGA+AGG + Chr4:74056552-74056571 None:intergenic 50.0%
AAGACGGCGAGCCGAGAAAT+TGG + Chr4:74053711-74053730 None:intergenic 55.0%
AGACGGCGAGCCGAGAAATT+GGG + Chr4:74053710-74053729 None:intergenic 55.0%
ATGTGGCTGACCCCACTTAG+TGG - Chr4:74055213-74055232 MsG0480022556.01.T01:intron 55.0%
CACCTCACTTCGTGGTCGTT+GGG - Chr4:74055399-74055418 MsG0480022556.01.T01:intron 55.0%
CTTCGAGGACTTCCTGCACA+TGG + Chr4:74056853-74056872 None:intergenic 55.0%
TCACCTCACTTCGTGGTCGT+TGG - Chr4:74055398-74055417 MsG0480022556.01.T01:intron 55.0%
TCGTCTCCTCGATAACCCGT+CGG - Chr4:74053677-74053696 MsG0480022556.01.T01:intron 55.0%
TGTGGCTGACCCCACTTAGT+GGG - Chr4:74055214-74055233 MsG0480022556.01.T01:intron 55.0%
TTCCCAACGACCACGAAGTG+AGG + Chr4:74055404-74055423 None:intergenic 55.0%
! ACGGGTTATCGAGGAGACGA+CGG + Chr4:74053677-74053696 None:intergenic 55.0%
! ATTTCTCGGCTCGCCGTCTT+CGG - Chr4:74053711-74053730 MsG0480022556.01.T01:intron 55.0%
! CGGAGCACCCAACAGCTTAT+TGG + Chr4:74054272-74054291 None:intergenic 55.0%
AGGAGACGACGGTGATGAGG+AGG + Chr4:74053666-74053685 None:intergenic 60.0%
GACGGCGAGCCGAGAAATTG+GGG + Chr4:74053709-74053728 None:intergenic 60.0%
GAGCCGAGAAATTGGGGCGA+CGG + Chr4:74053703-74053722 None:intergenic 60.0%
TCGAGGAGACGACGGTGATG+AGG + Chr4:74053669-74053688 None:intergenic 60.0%
AAATTGGGGCGACGGCCGAC+GGG + Chr4:74053695-74053714 None:intergenic 65.0%
CGGCCGTCGCCCCAATTTCT+CGG - Chr4:74053697-74053716 MsG0480022556.01.T01:intron 65.0%
GAAATTGGGGCGACGGCCGA+CGG + Chr4:74053696-74053715 None:intergenic 65.0%
!! CGACGGCCGACGGGTTATCG+AGG + Chr4:74053686-74053705 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
Chr4 gene 74053094 74056976 74053094 ID=MsG0480022556.01;Name=MsG0480022556.01
Chr4 mRNA 74053094 74056976 74053094 ID=MsG0480022556.01.T01;Parent=MsG0480022556.01;Name=MsG0480022556.01.T01;_AED=0.64;_eAED=0.75;_QI=0|0|0|0.66|0|0|3|0|175
Chr4 exon 74056802 74056976 74056802 ID=MsG0480022556.01.T01:exon:8468;Parent=MsG0480022556.01.T01
Chr4 exon 74055460 74055578 74055460 ID=MsG0480022556.01.T01:exon:8467;Parent=MsG0480022556.01.T01
Chr4 exon 74053094 74053327 74053094 ID=MsG0480022556.01.T01:exon:8466;Parent=MsG0480022556.01.T01
Chr4 CDS 74056802 74056976 74056802 ID=MsG0480022556.01.T01:cds;Parent=MsG0480022556.01.T01
Chr4 CDS 74055460 74055578 74055460 ID=MsG0480022556.01.T01:cds;Parent=MsG0480022556.01.T01
Chr4 CDS 74053094 74053327 74053094 ID=MsG0480022556.01.T01:cds;Parent=MsG0480022556.01.T01
Gene Sequence

>MsG0480022556.01.T01

ATGTCCACGTACAGAACTTTGTTATACAATTTACCGAAGCTAGTCCAAGGAGTGTATCAGAAGGAGGAGGACCCATCACCCAATCTAAAATCAAAACCTGGTCGTAAGACTGCTTTTGCTTTCATTACTGAATTCCATACTATGGATCCTTGTCTTTTCGGCATATCCAGAAAAGCCCCAGTTTATCAAATGCGGCAGTATTACAGGCTTGGTTCTCTTGATAACTGTCGCGGTAAATGGAAAGCATGGACTGACTGCTTAATGCTCAAGACCAAACCCAAATCTCAAGTCGAGATTAGATCACAAGTATTTGAGCGAAAACAACTCTCTTGGATCGTGTTTTTCCATTCTAGTGTTCTCCTACTTAACAAATGTTCTTGGAGTAACAAGCTATATGTAACCCATGTGCAGGAAGTCCTCGAAGCTCGTGAGAAATCAAAAAAGCCACACATCTGGAATTTTCGAACGCGATATGAAGCTTCACAAAACTGGCAAAAAATGTATGGTCATTTAGACAAGCCTGAATGA
Protein sequence

>MsG0480022556.01.T01

MSTYRTLLYNLPKLVQGVYQKEEDPSPNLKSKPGRKTAFAFITEFHTMDPCLFGISRKAPVYQMRQYYRLGSLDNCRGKWKAWTDCLMLKTKPKSQVEIRSQVFERKQLSWIVFFHSSVLLLNKCSWSNKLYVTHVQEVLEAREKSKKPHIWNFRTRYEASQNWQKMYGHLDKPE*