AlfalfaGEDB | MsG0480022641.01

M. sativa cultivar ZhongmuNo.1 / MsG0480022641.01

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480022641.01.T01	GAU30569.1	75.373	134	21	4	1	124	1	132	2.10E-39	139

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480022641.01.T01	A0A2Z6NFT7	75.373	134	21	4	1	124	1	132	1.00e-39	139

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0480022639.01	MsG0480022641.01	0.848254	6.889484e-60	1.256734e-56
MsG0480022641.01	MsG0480022642.01	0.821726	3.463893e-53	2.840951e-50

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480022641.01.T01	MTR_4g093825	92.593	108	8	0	1	108	1	108	4.30e-34	115
MsG0480022641.01.T01	MTR_4g093790	77.953	127	19	3	1	124	1	121	1.31e-32	111
MsG0480022641.01.T01	MTR_4g093835	72.727	132	18	3	1	124	1	122	2.57e-30	105
MsG0480022641.01.T01	MTR_4g093810	79.839	124	20	2	1	124	1	119	3.50e-27	97.4
MsG0480022641.01.T01	MTR_4g093780	65.347	101	30	2	1	98	1	99	4.06e-22	87.0
MsG0480022641.01.T01	MTR_6g039360	42.781	187	42	4	3	124	4	190	8.66e-19	77.8
MsG0480022641.01.T01	MTR_0002s0150	58.333	96	26	1	3	98	4	85	9.17e-15	69.7
MsG0480022641.01.T01	MTR_6g039850	65.574	61	21	0	38	98	22	82	9.90e-11	55.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 48 sgRNAs with CRISPR-Local

Find 47 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTGAACAGATTCACCTTTGC+CGG	0.313088	4:-74975329	MsG0480022641.01.T01:CDS
GATGCTTGTGGCTACACTAT+TGG	0.362100	4:-74975615	MsG0480022641.01.T01:CDS
AGGTGTTGCAACAGGGGATA+TGG	0.362150	4:+74975553	None:intergenic
AACCACAACAGCAGCAGATC+CGG	0.381665	4:+74975310	None:intergenic
AGGAGATGGTGCAGAGTTGA+TGG	0.390310	4:+74975451	None:intergenic
GAGATCGCAGGTGTTGCAAC+AGG	0.396955	4:+74975545	None:intergenic
GGAGGAGTGGAGATCGATGA+TGG	0.427396	4:+74975383	None:intergenic
CTCTCCCTCCGCTGACTCAC+CGG	0.429059	4:-74975525	MsG0480022641.01.T01:CDS
GCGGGAGAGTCGGAGGAAGC+AGG	0.432793	4:+74975425	None:intergenic
GGAGAGGGAGCGCTCTTCAC+AGG	0.474938	4:+74975485	None:intergenic
TGCCGGATCTGCTGCTGTTG+TGG	0.475100	4:-74975312	MsG0480022641.01.T01:CDS
GTTCAAAGCGGCGCCGTTTG+CGG	0.484242	4:+74975346	None:intergenic
TGAAGATGGAGATTGAGCGA+AGG	0.494303	4:+74975583	None:intergenic
AGGAGAGACAGCGGGAGAGT+CGG	0.494556	4:+74975415	None:intergenic
GATGGCAGATGGTGAAGGAG+AGG	0.497114	4:+74975469	None:intergenic
GGAGCGCTCTTCACAGGTAC+GGG	0.499222	4:+74975491	None:intergenic
TCTGAAGGTCCTTCCGCAAA+CGG	0.511949	4:-74975359	MsG0480022641.01.T01:CDS
TCAGCGGAGGGAGAGATCGC+AGG	0.514743	4:+74975533	None:intergenic
AGAGACAGCGGGAGAGTCGG+AGG	0.517005	4:+74975418	None:intergenic
ATCTCCACTCCTCCTTCTGA+AGG	0.524319	4:-74975374	MsG0480022641.01.T01:CDS
ATCGATGATGGAGTAACGGC+AGG	0.528907	4:+74975395	None:intergenic
GAGTCGGAGGAAGCAGGAGA+AGG	0.544934	4:+74975431	None:intergenic
GGGAGCGCTCTTCACAGGTA+CGG	0.546719	4:+74975490	None:intergenic
AAAGCGGCGCCGTTTGCGGA+AGG	0.548917	4:+74975350	None:intergenic
AGTAACGGCAGGAGAGACAG+CGG	0.553356	4:+74975406	None:intergenic
AGGACCTTCAGAAGGAGGAG+TGG	0.568330	4:+74975370	None:intergenic
TGCCACCGGTGAGTCAGCGG+AGG	0.568502	4:+74975520	None:intergenic
GTAACGGCAGGAGAGACAGC+GGG	0.589547	4:+74975407	None:intergenic
ATGGCAGATGGTGAAGGAGA+GGG	0.589863	4:+74975470	None:intergenic
AGATGCCACCGGTGAGTCAG+CGG	0.593129	4:+74975517	None:intergenic
TCCCTCCGCTGACTCACCGG+TGG	0.596452	4:-74975522	MsG0480022641.01.T01:CDS
CACAGTTGTGTTGATGCTTG+TGG	0.597734	4:-74975627	MsG0480022641.01.T01:CDS
GCGGAAGGACCTTCAGAAGG+AGG	0.598477	4:+74975365	None:intergenic
GAGGAAGCAGGAGAAGGAGA+TGG	0.598923	4:+74975437	None:intergenic
GATATGGTAGGAGATGAAGA+TGG	0.601445	4:+74975569	None:intergenic
GGAGATCGATGATGGAGTAA+CGG	0.602999	4:+74975391	None:intergenic
TTTGCGGAAGGACCTTCAGA+AGG	0.615822	4:+74975362	None:intergenic
GCGCTCTTCACAGGTACGGG+TGG	0.617846	4:+74975494	None:intergenic
GCCACCGGTGAGTCAGCGGA+GGG	0.624359	4:+74975521	None:intergenic
AGATCGCAGGTGTTGCAACA+GGG	0.627525	4:+74975546	None:intergenic
AACAGCAGCAGATCCGGCAA+AGG	0.628293	4:+74975316	None:intergenic
GTTGCAACAGGGGATATGGT+AGG	0.629950	4:+74975557	None:intergenic
GATCGCAGGTGTTGCAACAG+GGG	0.632727	4:+74975547	None:intergenic
GGTACGGGTGGAGATGCCAC+CGG	0.639491	4:+74975506	None:intergenic
GGTGCAGAGTTGATGGCAGA+TGG	0.654616	4:+74975458	None:intergenic
AACTCTCTACAGCATCAAAG+CGG	0.677293	4:+74975280	None:intergenic
GAGTTGATGGCAGATGGTGA+AGG	0.677440	4:+74975464	None:intergenic
AAAGGTGAATCTGTTCAAAG+CGG	0.702492	4:+74975334	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AAAGGTGAATCTGTTCAAAG+CGG	+	Chr4:74975593-74975612	None:intergenic	35.0%
	GATATGGTAGGAGATGAAGA+TGG	+	Chr4:74975358-74975377	None:intergenic	40.0%
	TTGAACAGATTCACCTTTGC+CGG	-	Chr4:74975595-74975614	MsG0480022641.01.T01:CDS	40.0%
	GATGCTTGTGGCTACACTAT+TGG	-	Chr4:74975309-74975328	MsG0480022641.01.T01:CDS	45.0%
	TGAAGATGGAGATTGAGCGA+AGG	+	Chr4:74975344-74975363	None:intergenic	45.0%
!	CACAGTTGTGTTGATGCTTG+TGG	-	Chr4:74975297-74975316	MsG0480022641.01.T01:CDS	45.0%
!!	GGAGATCGATGATGGAGTAA+CGG	+	Chr4:74975536-74975555	None:intergenic	45.0%
	AACCACAACAGCAGCAGATC+CGG	+	Chr4:74975617-74975636	None:intergenic	50.0%
	AGGAGATGGTGCAGAGTTGA+TGG	+	Chr4:74975476-74975495	None:intergenic	50.0%
	ATCTCCACTCCTCCTTCTGA+AGG	-	Chr4:74975550-74975569	MsG0480022641.01.T01:CDS	50.0%
	ATGGCAGATGGTGAAGGAGA+GGG	+	Chr4:74975457-74975476	None:intergenic	50.0%
	GTTGCAACAGGGGATATGGT+AGG	+	Chr4:74975370-74975389	None:intergenic	50.0%
	TCTGAAGGTCCTTCCGCAAA+CGG	-	Chr4:74975565-74975584	MsG0480022641.01.T01:CDS	50.0%
	TTTGCGGAAGGACCTTCAGA+AGG	+	Chr4:74975565-74975584	None:intergenic	50.0%
!	AGATCGCAGGTGTTGCAACA+GGG	+	Chr4:74975381-74975400	None:intergenic	50.0%
!	AGGTGTTGCAACAGGGGATA+TGG	+	Chr4:74975374-74975393	None:intergenic	50.0%
!!	ATCGATGATGGAGTAACGGC+AGG	+	Chr4:74975532-74975551	None:intergenic	50.0%
!!	GAGTTGATGGCAGATGGTGA+AGG	+	Chr4:74975463-74975482	None:intergenic	50.0%
	AACAGCAGCAGATCCGGCAA+AGG	+	Chr4:74975611-74975630	None:intergenic	55.0%
	AGGACCTTCAGAAGGAGGAG+TGG	+	Chr4:74975557-74975576	None:intergenic	55.0%
	AGTAACGGCAGGAGAGACAG+CGG	+	Chr4:74975521-74975540	None:intergenic	55.0%
	GAGGAAGCAGGAGAAGGAGA+TGG	+	Chr4:74975490-74975509	None:intergenic	55.0%
	GATGGCAGATGGTGAAGGAG+AGG	+	Chr4:74975458-74975477	None:intergenic	55.0%
	GGAGGAGTGGAGATCGATGA+TGG	+	Chr4:74975544-74975563	None:intergenic	55.0%
!	GAGATCGCAGGTGTTGCAAC+AGG	+	Chr4:74975382-74975401	None:intergenic	55.0%
!	GATCGCAGGTGTTGCAACAG+GGG	+	Chr4:74975380-74975399	None:intergenic	55.0%
!!	GGTGCAGAGTTGATGGCAGA+TGG	+	Chr4:74975469-74975488	None:intergenic	55.0%
	AGATGCCACCGGTGAGTCAG+CGG	+	Chr4:74975410-74975429	None:intergenic	60.0%
	AGGAGAGACAGCGGGAGAGT+CGG	+	Chr4:74975512-74975531	None:intergenic	60.0%
	GAGTCGGAGGAAGCAGGAGA+AGG	+	Chr4:74975496-74975515	None:intergenic	60.0%
	GCGGAAGGACCTTCAGAAGG+AGG	+	Chr4:74975562-74975581	None:intergenic	60.0%
	GTAACGGCAGGAGAGACAGC+GGG	+	Chr4:74975520-74975539	None:intergenic	60.0%
	GTTCAAAGCGGCGCCGTTTG+CGG	+	Chr4:74975581-74975600	None:intergenic	60.0%
	TGCCGGATCTGCTGCTGTTG+TGG	-	Chr4:74975612-74975631	MsG0480022641.01.T01:CDS	60.0%
!	GGAGCGCTCTTCACAGGTAC+GGG	+	Chr4:74975436-74975455	None:intergenic	60.0%
!!	GGGAGCGCTCTTCACAGGTA+CGG	+	Chr4:74975437-74975456	None:intergenic	60.0%
	AAAGCGGCGCCGTTTGCGGA+AGG	+	Chr4:74975577-74975596	None:intergenic	65.0%
	AGAGACAGCGGGAGAGTCGG+AGG	+	Chr4:74975509-74975528	None:intergenic	65.0%
	GCGCTCTTCACAGGTACGGG+TGG	+	Chr4:74975433-74975452	None:intergenic	65.0%
	GGTACGGGTGGAGATGCCAC+CGG	+	Chr4:74975421-74975440	None:intergenic	65.0%
	TCAGCGGAGGGAGAGATCGC+AGG	+	Chr4:74975394-74975413	None:intergenic	65.0%
!!	CTCTCCCTCCGCTGACTCAC+CGG	-	Chr4:74975399-74975418	MsG0480022641.01.T01:CDS	65.0%
!!	GGAGAGGGAGCGCTCTTCAC+AGG	+	Chr4:74975442-74975461	None:intergenic	65.0%
	GCCACCGGTGAGTCAGCGGA+GGG	+	Chr4:74975406-74975425	None:intergenic	70.0%
	GCGGGAGAGTCGGAGGAAGC+AGG	+	Chr4:74975502-74975521	None:intergenic	70.0%
	TGCCACCGGTGAGTCAGCGG+AGG	+	Chr4:74975407-74975426	None:intergenic	70.0%
!!	TCCCTCCGCTGACTCACCGG+TGG	-	Chr4:74975402-74975421	MsG0480022641.01.T01:CDS	70.0%

Chromosome	Type	Strat	End	Strand	Name
Chr4	gene	74975286	74975660	74975286	ID=MsG0480022641.01;Name=MsG0480022641.01
Chr4	mRNA	74975286	74975660	74975286	ID=MsG0480022641.01.T01;Parent=MsG0480022641.01;Name=MsG0480022641.01.T01;_AED=0.50;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|124
Chr4	exon	74975286	74975660	74975286	ID=MsG0480022641.01.T01:exon:31592;Parent=MsG0480022641.01.T01
Chr4	CDS	74975286	74975660	74975286	ID=MsG0480022641.01.T01:cds;Parent=MsG0480022641.01.T01

Gene Sequence

>MsG0480022641.01.T01

ATGGCTTCATACACAGTTGTGTTGATGCTTGTGGCTACACTATTGGTTAGTTCTACCTTCGCTCAATCTCCATCTTCATCTCCTACCATATCCCCTGTTGCAACACCTGCGATCTCTCCCTCCGCTGACTCACCGGTGGCATCTCCACCCGTACCTGTGAAGAGCGCTCCCTCTCCTTCACCATCTGCCATCAACTCTGCACCATCTCCTTCTCCTGCTTCCTCCGACTCTCCCGCTGTCTCTCCTGCCGTTACTCCATCATCGATCTCCACTCCTCCTTCTGAAGGTCCTTCCGCAAACGGCGCCGCTTTGAACAGATTCACCTTTGCCGGATCTGCTGCTGTTGTGGTTTTTGCTGCCGCTTTGATGCTGTAG

Protein sequence

>MsG0480022641.01.T01

MASYTVVLMLVATLLVSSTFAQSPSSSPTISPVATPAISPSADSPVASPPVPVKSAPSPSPSAINSAPSPSPASSDSPAVSPAVTPSSISTPPSEGPSANGAALNRFTFAGSAAVVVFAAALML*