Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480022639.01.T01 | GAU30569.1 | 72.388 | 134 | 26 | 5 | 1 | 125 | 1 | 132 | 6.22E-34 | 125 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480022639.01.T01 | A0A2Z6NFT7 | 72.388 | 134 | 26 | 5 | 1 | 125 | 1 | 132 | 2.97e-34 | 125 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0480022639.01 | MsG0480022641.01 | 0.848254 | 6.889484e-60 | 1.256734e-56 |
| MsG0480022639.01 | MsG0480022642.01 | 0.817231 | 3.670990e-52 | 2.658194e-49 |
| MsG0480022639.01 | MsG0580028909.01 | 0.800364 | 1.492132e-48 | 6.934904e-46 |
| MsG0380015862.01 | MsG0480022639.01 | 0.823287 | 1.502445e-53 | 1.288100e-50 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480022639.01.T01 | MTR_4g093810 | 88.800 | 125 | 8 | 1 | 1 | 125 | 1 | 119 | 1.01e-34 | 116 |
| MsG0480022639.01.T01 | MTR_4g093790 | 73.643 | 129 | 22 | 4 | 1 | 125 | 1 | 121 | 4.27e-30 | 105 |
| MsG0480022639.01.T01 | MTR_4g093835 | 70.455 | 132 | 22 | 3 | 1 | 125 | 1 | 122 | 4.05e-28 | 100 |
| MsG0480022639.01.T01 | MTR_4g093780 | 70.874 | 103 | 23 | 2 | 1 | 100 | 1 | 99 | 5.23e-28 | 102 |
| MsG0480022639.01.T01 | MTR_4g093825 | 83.636 | 110 | 15 | 2 | 1 | 109 | 1 | 108 | 3.92e-26 | 95.1 |
| MsG0480022639.01.T01 | MTR_6g039360 | 41.270 | 189 | 43 | 5 | 3 | 125 | 4 | 190 | 1.61e-14 | 67.0 |
| MsG0480022639.01.T01 | MTR_6g039850 | 65.574 | 61 | 21 | 0 | 40 | 100 | 22 | 82 | 2.75e-13 | 62.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 55 sgRNAs with CRISPR-Local
Find 53 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTGAATAGATTCACCGTTGC+CGG | 0.314871 | 4:-74949465 | MsG0480022639.01.T01:CDS |
| TGGAGTGGCAACGGGAGATT+TGG | 0.322110 | 4:+74949692 | None:intergenic |
| TCTCCTCCTCCTGCTTCCTC+TGG | 0.367416 | 4:-74949567 | MsG0480022639.01.T01:CDS |
| GATGCTTGTGGCTGCATTGT+TGG | 0.371395 | 4:-74949754 | MsG0480022639.01.T01:CDS |
| AGGAGATGGTGGAGAGTTGA+TGG | 0.371781 | 4:+74949584 | None:intergenic |
| AAGCATCAACACAACTTTGT+AGG | 0.415925 | 4:+74949770 | None:intergenic |
| AGTGACAGCAGGGGCGGCGG+CGG | 0.427837 | 4:+74949539 | None:intergenic |
| ATCGATGATGGAGTGACAGC+AGG | 0.443446 | 4:+74949528 | None:intergenic |
| GAAGCAGGAGGAGGAGATGG+TGG | 0.444045 | 4:+74949573 | None:intergenic |
| GCGGGAGATCCAGAGGAAGC+AGG | 0.447382 | 4:+74949558 | None:intergenic |
| GTGACAGCAGGGGCGGCGGC+GGG | 0.450586 | 4:+74949540 | None:intergenic |
| AACCACAACAGCAGCAGATC+CGG | 0.457034 | 4:+74949446 | None:intergenic |
| CCGTTCGATGGTGCTTCAGC+AGG | 0.467052 | 4:+74949495 | None:intergenic |
| GCAGGAGATGGAGTGGCAAC+GGG | 0.467415 | 4:+74949684 | None:intergenic |
| TGCCGGATCTGCTGCTGTTG+TGG | 0.475100 | 4:-74949448 | MsG0480022639.01.T01:CDS |
| TCACCGGAGGGAGAGATCGC+AGG | 0.481584 | 4:+74949666 | None:intergenic |
| CGCAGGAGATGGAGTGGCAA+CGG | 0.487389 | 4:+74949683 | None:intergenic |
| TGGAGTGACAGCAGGGGCGG+CGG | 0.489307 | 4:+74949536 | None:intergenic |
| GAGGAAGCAGGAGGAGGAGA+TGG | 0.494965 | 4:+74949570 | None:intergenic |
| GGAGTATTCTTCACAGGTGC+AGG | 0.495672 | 4:+74949624 | None:intergenic |
| GGAGGAGTGGAGATCGATGA+TGG | 0.496417 | 4:+74949516 | None:intergenic |
| TTCAAAGCGGCGCCGTTCGA+TGG | 0.497857 | 4:+74949483 | None:intergenic |
| CTCTCCCTCCGGTGACTCAC+CGG | 0.499775 | 4:-74949658 | MsG0480022639.01.T01:CDS |
| GAGGGAGAGATCGCAGGAGA+TGG | 0.500935 | 4:+74949672 | None:intergenic |
| AGATGCCGCCGGTGAGTCAC+CGG | 0.514366 | 4:+74949650 | None:intergenic |
| GATCCAGAGGAAGCAGGAGG+AGG | 0.530807 | 4:+74949564 | None:intergenic |
| CAAAGTTGTGTTGATGCTTG+TGG | 0.536413 | 4:-74949766 | MsG0480022639.01.T01:CDS |
| TTCGATGGTGCTTCAGCAGG+AGG | 0.539403 | 4:+74949498 | None:intergenic |
| GGCGGCGGCGGGAGATCCAG+AGG | 0.539861 | 4:+74949551 | None:intergenic |
| ATGGTGGATGGTGAAGGAGA+TGG | 0.550636 | 4:+74949603 | None:intergenic |
| TCTCCTGCGATCTCTCCCTC+CGG | 0.559625 | 4:-74949669 | MsG0480022639.01.T01:CDS |
| TCGATGATGGAGTGACAGCA+GGG | 0.563807 | 4:+74949529 | None:intergenic |
| GGAGATGGAGTATTCTTCAC+AGG | 0.569370 | 4:+74949618 | None:intergenic |
| AGAGATCGCAGGAGATGGAG+TGG | 0.573319 | 4:+74949677 | None:intergenic |
| CGATGGTGATTGAGCGAAGG+TGG | 0.579542 | 4:+74949725 | None:intergenic |
| GGAGATCCAGAGGAAGCAGG+AGG | 0.586852 | 4:+74949561 | None:intergenic |
| GTGGCAACGGGAGATTTGGA+TGG | 0.591213 | 4:+74949696 | None:intergenic |
| GGTGCAGGAGGAGATGCCGC+CGG | 0.600074 | 4:+74949639 | None:intergenic |
| AGATGGTGGAGAGTTGATGG+TGG | 0.602459 | 4:+74949587 | None:intergenic |
| TGATGGAGTGACAGCAGGGG+CGG | 0.602853 | 4:+74949533 | None:intergenic |
| TGGTGCTTCAGCAGGAGGAG+TGG | 0.604768 | 4:+74949503 | None:intergenic |
| TGCCGCCGGTGAGTCACCGG+AGG | 0.606824 | 4:+74949653 | None:intergenic |
| GGTGGAGAGTTGATGGTGGA+TGG | 0.609584 | 4:+74949591 | None:intergenic |
| CCTGCTGAAGCACCATCGAA+CGG | 0.619717 | 4:-74949495 | MsG0480022639.01.T01:CDS |
| GAGTTGATGGTGGATGGTGA+AGG | 0.628457 | 4:+74949597 | None:intergenic |
| AACAGCAGCAGATCCGGCAA+CGG | 0.637005 | 4:+74949452 | None:intergenic |
| GTATTCTTCACAGGTGCAGG+AGG | 0.640194 | 4:+74949627 | None:intergenic |
| CGATGATGGAGTGACAGCAG+GGG | 0.655107 | 4:+74949530 | None:intergenic |
| GCCGCCGGTGAGTCACCGGA+GGG | 0.661844 | 4:+74949654 | None:intergenic |
| AACGGTGAATCTATTCAAAG+CGG | 0.675574 | 4:+74949470 | None:intergenic |
| TCCCTCCGGTGACTCACCGG+CGG | 0.676379 | 4:-74949655 | MsG0480022639.01.T01:CDS |
| TGACGATGGTGATTGAGCGA+AGG | 0.684662 | 4:+74949722 | None:intergenic |
| TCTCTACATCATCAAAGCGG+CGG | 0.692163 | 4:+74949419 | None:intergenic |
| AACTCTCTACATCATCAAAG+CGG | 0.713170 | 4:+74949416 | None:intergenic |
| GATTTGGATGGAGATGACGA+TGG | 0.743905 | 4:+74949708 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AACGGTGAATCTATTCAAAG+CGG | + | Chr4:74949732-74949751 | None:intergenic | 35.0% | |
| AAGCATCAACACAACTTTGT+AGG | + | Chr4:74949432-74949451 | None:intergenic | 35.0% | |
| TTGAATAGATTCACCGTTGC+CGG | - | Chr4:74949734-74949753 | MsG0480022639.01.T01:CDS | 40.0% | |
| ! | CAAAGTTGTGTTGATGCTTG+TGG | - | Chr4:74949433-74949452 | MsG0480022639.01.T01:CDS | 40.0% |
| GATTTGGATGGAGATGACGA+TGG | + | Chr4:74949494-74949513 | None:intergenic | 45.0% | |
| ! | GGAGATGGAGTATTCTTCAC+AGG | + | Chr4:74949584-74949603 | None:intergenic | 45.0% |
| AACCACAACAGCAGCAGATC+CGG | + | Chr4:74949756-74949775 | None:intergenic | 50.0% | |
| GATGCTTGTGGCTGCATTGT+TGG | - | Chr4:74949445-74949464 | MsG0480022639.01.T01:CDS | 50.0% | |
| GTATTCTTCACAGGTGCAGG+AGG | + | Chr4:74949575-74949594 | None:intergenic | 50.0% | |
| TGACGATGGTGATTGAGCGA+AGG | + | Chr4:74949480-74949499 | None:intergenic | 50.0% | |
| ! | GGAGTATTCTTCACAGGTGC+AGG | + | Chr4:74949578-74949597 | None:intergenic | 50.0% |
| !! | AGATGGTGGAGAGTTGATGG+TGG | + | Chr4:74949615-74949634 | None:intergenic | 50.0% |
| !! | AGGAGATGGTGGAGAGTTGA+TGG | + | Chr4:74949618-74949637 | None:intergenic | 50.0% |
| !! | ATCGATGATGGAGTGACAGC+AGG | + | Chr4:74949674-74949693 | None:intergenic | 50.0% |
| !! | ATGGTGGATGGTGAAGGAGA+TGG | + | Chr4:74949599-74949618 | None:intergenic | 50.0% |
| !! | GAGTTGATGGTGGATGGTGA+AGG | + | Chr4:74949605-74949624 | None:intergenic | 50.0% |
| !! | TCGATGATGGAGTGACAGCA+GGG | + | Chr4:74949673-74949692 | None:intergenic | 50.0% |
| AACAGCAGCAGATCCGGCAA+CGG | + | Chr4:74949750-74949769 | None:intergenic | 55.0% | |
| AGAGATCGCAGGAGATGGAG+TGG | + | Chr4:74949525-74949544 | None:intergenic | 55.0% | |
| CGATGGTGATTGAGCGAAGG+TGG | + | Chr4:74949477-74949496 | None:intergenic | 55.0% | |
| GGAGGAGTGGAGATCGATGA+TGG | + | Chr4:74949686-74949705 | None:intergenic | 55.0% | |
| GTGGCAACGGGAGATTTGGA+TGG | + | Chr4:74949506-74949525 | None:intergenic | 55.0% | |
| TGGAGTGGCAACGGGAGATT+TGG | + | Chr4:74949510-74949529 | None:intergenic | 55.0% | |
| TTCGATGGTGCTTCAGCAGG+AGG | + | Chr4:74949704-74949723 | None:intergenic | 55.0% | |
| ! | CCTGCTGAAGCACCATCGAA+CGG | - | Chr4:74949704-74949723 | MsG0480022639.01.T01:CDS | 55.0% |
| !! | CGATGATGGAGTGACAGCAG+GGG | + | Chr4:74949672-74949691 | None:intergenic | 55.0% |
| !! | GGTGGAGAGTTGATGGTGGA+TGG | + | Chr4:74949611-74949630 | None:intergenic | 55.0% |
| CCGTTCGATGGTGCTTCAGC+AGG | + | Chr4:74949707-74949726 | None:intergenic | 60.0% | |
| CGCAGGAGATGGAGTGGCAA+CGG | + | Chr4:74949519-74949538 | None:intergenic | 60.0% | |
| GAAGCAGGAGGAGGAGATGG+TGG | + | Chr4:74949629-74949648 | None:intergenic | 60.0% | |
| GAGGAAGCAGGAGGAGGAGA+TGG | + | Chr4:74949632-74949651 | None:intergenic | 60.0% | |
| GAGGGAGAGATCGCAGGAGA+TGG | + | Chr4:74949530-74949549 | None:intergenic | 60.0% | |
| GATCCAGAGGAAGCAGGAGG+AGG | + | Chr4:74949638-74949657 | None:intergenic | 60.0% | |
| GCAGGAGATGGAGTGGCAAC+GGG | + | Chr4:74949518-74949537 | None:intergenic | 60.0% | |
| GGAGATCCAGAGGAAGCAGG+AGG | + | Chr4:74949641-74949660 | None:intergenic | 60.0% | |
| TCTCCTCCTCCTGCTTCCTC+TGG | - | Chr4:74949632-74949651 | MsG0480022639.01.T01:CDS | 60.0% | |
| TCTCCTGCGATCTCTCCCTC+CGG | - | Chr4:74949530-74949549 | MsG0480022639.01.T01:CDS | 60.0% | |
| TGATGGAGTGACAGCAGGGG+CGG | + | Chr4:74949669-74949688 | None:intergenic | 60.0% | |
| TGCCGGATCTGCTGCTGTTG+TGG | - | Chr4:74949751-74949770 | MsG0480022639.01.T01:CDS | 60.0% | |
| TGGTGCTTCAGCAGGAGGAG+TGG | + | Chr4:74949699-74949718 | None:intergenic | 60.0% | |
| TTCAAAGCGGCGCCGTTCGA+TGG | + | Chr4:74949719-74949738 | None:intergenic | 60.0% | |
| AGATGCCGCCGGTGAGTCAC+CGG | + | Chr4:74949552-74949571 | None:intergenic | 65.0% | |
| CTCTCCCTCCGGTGACTCAC+CGG | - | Chr4:74949541-74949560 | MsG0480022639.01.T01:CDS | 65.0% | |
| GCGGGAGATCCAGAGGAAGC+AGG | + | Chr4:74949644-74949663 | None:intergenic | 65.0% | |
| TCACCGGAGGGAGAGATCGC+AGG | + | Chr4:74949536-74949555 | None:intergenic | 65.0% | |
| GGTGCAGGAGGAGATGCCGC+CGG | + | Chr4:74949563-74949582 | None:intergenic | 70.0% | |
| TCCCTCCGGTGACTCACCGG+CGG | - | Chr4:74949544-74949563 | MsG0480022639.01.T01:CDS | 70.0% | |
| TGGAGTGACAGCAGGGGCGG+CGG | + | Chr4:74949666-74949685 | None:intergenic | 70.0% | |
| AGTGACAGCAGGGGCGGCGG+CGG | + | Chr4:74949663-74949682 | None:intergenic | 75.0% | |
| GCCGCCGGTGAGTCACCGGA+GGG | + | Chr4:74949548-74949567 | None:intergenic | 75.0% | |
| TGCCGCCGGTGAGTCACCGG+AGG | + | Chr4:74949549-74949568 | None:intergenic | 75.0% | |
| ! | GGCGGCGGCGGGAGATCCAG+AGG | + | Chr4:74949651-74949670 | None:intergenic | 80.0% |
| ! | GTGACAGCAGGGGCGGCGGC+GGG | + | Chr4:74949662-74949681 | None:intergenic | 80.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 74949422 | 74949799 | 74949422 | ID=MsG0480022639.01;Name=MsG0480022639.01 |
| Chr4 | mRNA | 74949422 | 74949799 | 74949422 | ID=MsG0480022639.01.T01;Parent=MsG0480022639.01;Name=MsG0480022639.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|125 |
| Chr4 | exon | 74949422 | 74949799 | 74949422 | ID=MsG0480022639.01.T01:exon:31589;Parent=MsG0480022639.01.T01 |
| Chr4 | CDS | 74949422 | 74949799 | 74949422 | ID=MsG0480022639.01.T01:cds;Parent=MsG0480022639.01.T01 |
| Gene Sequence |
| Protein sequence |
