AlfalfaGEDB | MsG0480022642.01

M. sativa cultivar ZhongmuNo.1 / MsG0480022642.01

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480022642.01.T01	XP_013457178.1	95.082	122	6	0	1	122	1	122	6.04E-47	157
MsG0480022642.01.T02	XP_013457178.1	87.879	132	6	1	1	132	1	122	1.14E-43	149

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480022642.01.T01	A0A072UN30	95.082	122	6	0	1	122	1	122	2.88e-47	157
MsG0480022642.01.T02	A0A072UN30	87.879	132	6	1	1	132	1	122	5.43e-44	149

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0480022639.01	MsG0480022642.01	0.817231	3.670990e-52	2.658194e-49
MsG0480022641.01	MsG0480022642.01	0.821726	3.463893e-53	2.840951e-50
MsG0480022642.01	MsG0580027875.01	0.806452	8.170306e-50	4.436346e-47
MsG0480022642.01	MsG0880047494.01	0.805442	1.332615e-49	7.050806e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480022642.01.T01	MTR_4g093835	95.082	122	6	0	1	122	1	122	7.31e-51	157
MsG0480022642.01.T01	MTR_4g093790	77.344	128	16	3	1	122	1	121	1.19e-30	106
MsG0480022642.01.T01	MTR_4g093810	62.879	132	26	3	1	122	1	119	3.41e-20	79.7
MsG0480022642.01.T01	MTR_4g093825	68.966	116	18	3	1	106	1	108	8.20e-20	79.0
MsG0480022642.01.T01	MTR_4g093780	59.813	107	23	3	1	94	1	100	2.11e-17	74.7
MsG0480022642.01.T01	MTR_6g039360	76.000	50	12	0	73	122	141	190	1.71e-15	69.3
MsG0480022642.01.T02	MTR_4g093835	87.879	132	6	1	1	132	1	122	1.38e-47	149
MsG0480022642.01.T02	MTR_4g093790	78.030	132	18	3	1	132	1	121	2.57e-33	113
MsG0480022642.01.T02	MTR_4g093780	67.290	107	25	3	1	104	1	100	5.71e-24	92.0
MsG0480022642.01.T02	MTR_4g093810	73.485	132	22	2	1	132	1	119	1.87e-21	83.2
MsG0480022642.01.T02	MTR_4g093825	81.034	116	14	2	1	116	1	108	5.40e-21	82.0
MsG0480022642.01.T02	MTR_6g039360	41.146	192	46	4	3	132	4	190	4.45e-17	73.9
MsG0480022642.01.T02	MTR_6g039850	72.581	62	17	0	44	105	23	84	1.79e-13	63.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 48 sgRNAs with CRISPR-Local

Find 48 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTGAACAGATTCACATTTGC+TGG	0.304728	4:-74987027	MsG0480022642.01.T02:CDS
AGGAGATGGTGGAGAGTTGA+TGG	0.321290	4:+74987158	None:intergenic
TGGAGTGGCAACCGGTGATA+TGG	0.388096	4:+74987275	None:intergenic
CACAGTTGTGTTGATGTTTG+TGG	0.412350	4:-74987349	MsG0480022642.01.T02:CDS
GATGTTTGTGGCTACACTAT+TGG	0.415229	4:-74987337	MsG0480022642.01.T02:CDS
AGATGGTGCCTGAGAGGACT+TGG	0.427679	4:+74987251	None:intergenic
ATCTCCTTCCGCTGTCTCAC+CGG	0.436937	4:-74987232	MsG0480022642.01.T02:CDS
GGAGTAGTAGAGATGGATGA+TGG	0.440924	4:+74987090	None:intergenic
GAAGCAGGAGGAGGAGATGG+TGG	0.444045	4:+74987147	None:intergenic
GACTTGGGTGGAGTGGCAAC+CGG	0.450462	4:+74987267	None:intergenic
GGAGCGTTCTTCACAGGTAC+TGG	0.455392	4:+74987198	None:intergenic
CACTCCACCCAAGTCCTCTC+AGG	0.464764	4:-74987259	MsG0480022642.01.T02:CDS
TTGAGAAGGAGTAGTAGAGA+TGG	0.490041	4:+74987083	None:intergenic
GCGGGAGAGTCGGATGAAGC+AGG	0.506808	4:+74987132	None:intergenic
GGAGATGGAGCGTTCTTCAC+AGG	0.508776	4:+74987192	None:intergenic
TGCTGGATCTGCTGCCGTTG+TGG	0.511630	4:-74987010	MsG0480022642.01.T02:CDS
AGGAGAGACAGCGGGAGAGT+CGG	0.511796	4:+74987122	None:intergenic
ATGGATGATGGAGTAACAGC+AGG	0.530730	4:+74987102	None:intergenic
GAGTCGGATGAAGCAGGAGG+AGG	0.537705	4:+74987138	None:intergenic
GATGAAGCAGGAGGAGGAGA+TGG	0.538341	4:+74987144	None:intergenic
AGTAACAGCAGGAGAGACAG+CGG	0.550973	4:+74987113	None:intergenic
ATGGCAGATGGTGAAGGAGA+TGG	0.559733	4:+74987177	None:intergenic
GAAGGTGAAGGTGCTTGAGA+AGG	0.564001	4:+74987069	None:intergenic
GGTGAGACAGCGGAAGGAGA+TGG	0.565932	4:+74987234	None:intergenic
ATCATCTCCAACCATATCAC+CGG	0.577596	4:-74987286	MsG0480022642.01.T02:CDS
TCCTTCCGCTGTCTCACCGG+TGG	0.578332	4:-74987229	MsG0480022642.01.T02:CDS
GTAACAGCAGGAGAGACAGC+GGG	0.589547	4:+74987114	None:intergenic
CTGAGAGGACTTGGGTGGAG+TGG	0.594704	4:+74987260	None:intergenic
GATGGTGCCTGAGAGGACTT+GGG	0.602114	4:+74987252	None:intergenic
GCCACCGGTGAGACAGCGGA+AGG	0.606446	4:+74987228	None:intergenic
GAATCTGTTCAAAGCGGCGG+CGG	0.606447	4:+74987038	None:intergenic
GAGTTGATGGCAGATGGTGA+AGG	0.608419	4:+74987171	None:intergenic
GGTGGAGAGTTGATGGCAGA+TGG	0.609584	4:+74987165	None:intergenic
AGATGCCACCGGTGAGACAG+CGG	0.622483	4:+74987224	None:intergenic
TGAAGGAGATTGAGCAATGG+TGG	0.623656	4:+74987308	None:intergenic
GGAAGGAGATGGTGCCTGAG+AGG	0.629137	4:+74987245	None:intergenic
GTGGCAACCGGTGATATGGT+TGG	0.632011	4:+74987279	None:intergenic
TGTGAATCTGTTCAAAGCGG+CGG	0.637759	4:+74987035	None:intergenic
GGAGAGTCGGATGAAGCAGG+AGG	0.644433	4:+74987135	None:intergenic
GCGGCGGCGGAGTTATCAGA+AGG	0.647391	4:+74987051	None:intergenic
GGTACTGGAGGAGATGCCAC+CGG	0.659674	4:+74987213	None:intergenic
GCGGAGTTATCAGAAGGTGA+AGG	0.662844	4:+74987057	None:intergenic
GCGTTCTTCACAGGTACTGG+AGG	0.664174	4:+74987201	None:intergenic
GATATGGTTGGAGATGATGA+AGG	0.665449	4:+74987291	None:intergenic
AGCAGCAGCGAAAACCACAA+CGG	0.677280	4:+74986996	None:intergenic
TGATGAAGGAGATTGAGCAA+TGG	0.691682	4:+74987305	None:intergenic
GGTGCCTGAGAGGACTTGGG+TGG	0.711883	4:+74987255	None:intergenic
AAATGTGAATCTGTTCAAAG+CGG	0.718772	4:+74987032	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AAATGTGAATCTGTTCAAAG+CGG	+	Chr4:74987315-74987334	None:intergenic	30.0%
	TTGAACAGATTCACATTTGC+TGG	-	Chr4:74987317-74987336	MsG0480022642.01.T02:CDS	35.0%
	ATCATCTCCAACCATATCAC+CGG	-	Chr4:74987058-74987077	MsG0480022642.01.T02:CDS	40.0%
	GATATGGTTGGAGATGATGA+AGG	+	Chr4:74987056-74987075	None:intergenic	40.0%
	GATGTTTGTGGCTACACTAT+TGG	-	Chr4:74987007-74987026	MsG0480022642.01.T02:CDS	40.0%
	TGATGAAGGAGATTGAGCAA+TGG	+	Chr4:74987042-74987061	None:intergenic	40.0%
	TTGAGAAGGAGTAGTAGAGA+TGG	+	Chr4:74987264-74987283	None:intergenic	40.0%
!	CACAGTTGTGTTGATGTTTG+TGG	-	Chr4:74986995-74987014	MsG0480022642.01.T02:CDS	40.0%
	GGAGTAGTAGAGATGGATGA+TGG	+	Chr4:74987257-74987276	None:intergenic	45.0%
	TGAAGGAGATTGAGCAATGG+TGG	+	Chr4:74987039-74987058	None:intergenic	45.0%
	TGTGAATCTGTTCAAAGCGG+CGG	+	Chr4:74987312-74987331	None:intergenic	45.0%
!!	ATGGATGATGGAGTAACAGC+AGG	+	Chr4:74987245-74987264	None:intergenic	45.0%
	AGCAGCAGCGAAAACCACAA+CGG	+	Chr4:74987351-74987370	None:intergenic	50.0%
	AGTAACAGCAGGAGAGACAG+CGG	+	Chr4:74987234-74987253	None:intergenic	50.0%
	ATGGCAGATGGTGAAGGAGA+TGG	+	Chr4:74987170-74987189	None:intergenic	50.0%
	GCGGAGTTATCAGAAGGTGA+AGG	+	Chr4:74987290-74987309	None:intergenic	50.0%
!!	AGGAGATGGTGGAGAGTTGA+TGG	+	Chr4:74987189-74987208	None:intergenic	50.0%
!!	GAAGGTGAAGGTGCTTGAGA+AGG	+	Chr4:74987278-74987297	None:intergenic	50.0%
!!	GAGTTGATGGCAGATGGTGA+AGG	+	Chr4:74987176-74987195	None:intergenic	50.0%
	AGATGGTGCCTGAGAGGACT+TGG	+	Chr4:74987096-74987115	None:intergenic	55.0%
	ATCTCCTTCCGCTGTCTCAC+CGG	-	Chr4:74987112-74987131	MsG0480022642.01.T02:CDS	55.0%
	GAATCTGTTCAAAGCGGCGG+CGG	+	Chr4:74987309-74987328	None:intergenic	55.0%
	GATGAAGCAGGAGGAGGAGA+TGG	+	Chr4:74987203-74987222	None:intergenic	55.0%
	GATGGTGCCTGAGAGGACTT+GGG	+	Chr4:74987095-74987114	None:intergenic	55.0%
	GTAACAGCAGGAGAGACAGC+GGG	+	Chr4:74987233-74987252	None:intergenic	55.0%
	GTGGCAACCGGTGATATGGT+TGG	+	Chr4:74987068-74987087	None:intergenic	55.0%
	TGGAGTGGCAACCGGTGATA+TGG	+	Chr4:74987072-74987091	None:intergenic	55.0%
!	GGAGATGGAGCGTTCTTCAC+AGG	+	Chr4:74987155-74987174	None:intergenic	55.0%
!	GGAGCGTTCTTCACAGGTAC+TGG	+	Chr4:74987149-74987168	None:intergenic	55.0%
!!	GCGTTCTTCACAGGTACTGG+AGG	+	Chr4:74987146-74987165	None:intergenic	55.0%
!!	GGTGGAGAGTTGATGGCAGA+TGG	+	Chr4:74987182-74987201	None:intergenic	55.0%
	AGATGCCACCGGTGAGACAG+CGG	+	Chr4:74987123-74987142	None:intergenic	60.0%
	AGGAGAGACAGCGGGAGAGT+CGG	+	Chr4:74987225-74987244	None:intergenic	60.0%
	CACTCCACCCAAGTCCTCTC+AGG	-	Chr4:74987085-74987104	MsG0480022642.01.T02:CDS	60.0%
	CTGAGAGGACTTGGGTGGAG+TGG	+	Chr4:74987087-74987106	None:intergenic	60.0%
	GAAGCAGGAGGAGGAGATGG+TGG	+	Chr4:74987200-74987219	None:intergenic	60.0%
	GACTTGGGTGGAGTGGCAAC+CGG	+	Chr4:74987080-74987099	None:intergenic	60.0%
	GAGTCGGATGAAGCAGGAGG+AGG	+	Chr4:74987209-74987228	None:intergenic	60.0%
	GGAAGGAGATGGTGCCTGAG+AGG	+	Chr4:74987102-74987121	None:intergenic	60.0%
	GGAGAGTCGGATGAAGCAGG+AGG	+	Chr4:74987212-74987231	None:intergenic	60.0%
	GGTGAGACAGCGGAAGGAGA+TGG	+	Chr4:74987113-74987132	None:intergenic	60.0%
	TGCTGGATCTGCTGCCGTTG+TGG	-	Chr4:74987334-74987353	MsG0480022642.01.T02:CDS	60.0%
!	GGTACTGGAGGAGATGCCAC+CGG	+	Chr4:74987134-74987153	None:intergenic	60.0%
	GCGGCGGCGGAGTTATCAGA+AGG	+	Chr4:74987296-74987315	None:intergenic	65.0%
	GCGGGAGAGTCGGATGAAGC+AGG	+	Chr4:74987215-74987234	None:intergenic	65.0%
	GGTGCCTGAGAGGACTTGGG+TGG	+	Chr4:74987092-74987111	None:intergenic	65.0%
	TCCTTCCGCTGTCTCACCGG+TGG	-	Chr4:74987115-74987134	MsG0480022642.01.T02:CDS	65.0%
	GCCACCGGTGAGACAGCGGA+AGG	+	Chr4:74987119-74987138	None:intergenic	70.0%

Chromosome	Type	Strat	End	Strand	Name
Chr4	gene	74986984	74987382	74986984	ID=MsG0480022642.01;Name=MsG0480022642.01
Chr4	mRNA	74986984	74987382	74986984	ID=MsG0480022642.01.T01;Parent=MsG0480022642.01;Name=MsG0480022642.01.T01;_AED=0.50;_eAED=0.50;_QI=0\|0\|0\|1\|0\|0\|2\|0\|122
Chr4	exon	74986984	74987150	74986984	ID=MsG0480022642.01.T01:exon:31594;Parent=MsG0480022642.01.T01
Chr4	exon	74987181	74987382	74987181	ID=MsG0480022642.01.T01:exon:31593;Parent=MsG0480022642.01.T01
Chr4	CDS	74987181	74987382	74987181	ID=MsG0480022642.01.T01:cds;Parent=MsG0480022642.01.T01
Chr4	CDS	74986984	74987150	74986984	ID=MsG0480022642.01.T01:cds;Parent=MsG0480022642.01.T01
Chr4	mRNA	74986984	74987382	74986984	ID=MsG0480022642.01.T02;Parent=MsG0480022642.01;Name=MsG0480022642.01.T02;_AED=0.50;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|132
Chr4	exon	74986984	74987382	74986984	ID=MsG0480022642.01.T02:exon:31595;Parent=MsG0480022642.01.T02
Chr4	CDS	74986984	74987382	74986984	ID=MsG0480022642.01.T02:cds;Parent=MsG0480022642.01.T02

Gene Sequence

>MsG0480022642.01.T01

ATGGCTTCATACACAGTTGTGTTGATGTTTGTGGCTACACTATTGGTTAGTTCCACCATTGCTCAATCTCCTTCATCATCTCCAACCATATCACCGGTTGCCACTCCACCCAAGTCCTCTCAGGCACCATCTCCTTCCGCTGTCTCACCGGTGGCATCTCCTCCAGTACCTGTGAAGAACGCTCCATCTCCTTCACCATCTGCTTCATCCGACTCTCCCGCTGTCTCTCCTGCTGTTACTCCATCATCCATCTCTACTACTCCTTCTCAAGCACCTTCACCTTCTGATAACTCCGCCGCCGCTTTGAACAGATTCACATTTGCTGGATCTGCTGCCGTTGTGGTTTTCGCTGCTGCTTTGATGATGTAG

>MsG0480022642.01.T02

ATGGCTTCATACACAGTTGTGTTGATGTTTGTGGCTACACTATTGGTTAGTTCCACCATTGCTCAATCTCCTTCATCATCTCCAACCATATCACCGGTTGCCACTCCACCCAAGTCCTCTCAGGCACCATCTCCTTCCGCTGTCTCACCGGTGGCATCTCCTCCAGTACCTGTGAAGAACGCTCCATCTCCTTCACCATCTGCCATCAACTCTCCACCATCTCCTCCTCCTGCTTCATCCGACTCTCCCGCTGTCTCTCCTGCTGTTACTCCATCATCCATCTCTACTACTCCTTCTCAAGCACCTTCACCTTCTGATAACTCCGCCGCCGCTTTGAACAGATTCACATTTGCTGGATCTGCTGCCGTTGTGGTTTTCGCTGCTGCTTTGATGATGTAG

Protein sequence

>MsG0480022642.01.T01

MASYTVVLMFVATLLVSSTIAQSPSSSPTISPVATPPKSSQAPSPSAVSPVASPPVPVKNAPSPSPSASSDSPAVSPAVTPSSISTTPSQAPSPSDNSAAALNRFTFAGSAAVVVFAAALMM*

>MsG0480022642.01.T02

MASYTVVLMFVATLLVSSTIAQSPSSSPTISPVATPPKSSQAPSPSAVSPVASPPVPVKNAPSPSPSAINSPPSPPPASSDSPAVSPAVTPSSISTTPSQAPSPSDNSAAALNRFTFAGSAAVVVFAAALMM*