AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480023309.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480023309.01.T01 MTR_4g094615 82.018 228 39 2 1 226 233 460 3.32e-129 372
MsG0480023309.01.T01 MTR_4g094615 83.212 137 23 0 1 137 233 369 8.04e-77 236
MsG0480023309.01.T01 MTR_4g094615 83.212 137 23 0 1 137 233 369 2.30e-76 236
MsG0480023309.01.T01 MTR_8g090145 72.067 179 38 4 52 226 24 194 1.87e-70 214
MsG0480023309.01.T02 MTR_4g094615 63.402 194 20 1 1 143 1 194 1.26e-64 202
MsG0480023309.01.T02 MTR_4g094615 63.402 194 20 2 1 143 1 194 1.99e-64 203
MsG0480023309.01.T02 MTR_4g094615 63.402 194 20 1 1 143 1 194 2.89e-64 201
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480023309.01.T01 AT3G51270 43.145 248 100 9 1 226 233 461 1.21e-35 131
MsG0480023309.01.T01 AT3G51270 43.145 248 100 9 1 226 233 461 1.21e-35 131
MsG0480023309.01.T01 AT3G51270 43.145 248 100 9 1 226 233 461 1.21e-35 131
MsG0480023309.01.T01 AT3G51270 42.972 249 104 9 1 226 225 458 2.18e-35 130
MsG0480023309.01.T02 AT3G51270 58.247 194 30 2 1 143 1 194 9.21e-62 196
MsG0480023309.01.T02 AT3G51270 58.247 194 30 2 1 143 1 194 9.21e-62 196
MsG0480023309.01.T02 AT3G51270 58.247 194 30 2 1 143 1 194 9.21e-62 196
MsG0480023309.01.T02 AT3G51270 56.316 190 32 2 1 139 1 190 7.35e-56 181

Find 54 sgRNAs with CRISPR-Local

Find 239 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACATCAAAGTAGTTATAAT+TGG 0.238386 4:-83602866 MsG0480023309.01.T01:five_prime_UTR
CGGTTCACAGCCTCCGGAAC+TGG 0.302352 4:+83602691 None:intergenic
TTAAATCGTCTGCTTCATTC+AGG 0.317069 4:+83599552 None:intergenic
CAATTATCGGTATAATTATT+CGG 0.330963 4:-83601751 MsG0480023309.01.T01:five_prime_UTR
GATAACGACGATTCTGATAA+AGG 0.348942 4:-83599856 MsG0480023309.01.T01:CDS
TGTTGATTCTTTGCATTTGT+CGG 0.381133 4:-83599528 MsG0480023309.01.T01:CDS
ATCTATCTGCAGTTTATTGA+AGG 0.411095 4:-83599629 MsG0480023309.01.T01:intron
TGATTACACAATGTCGATTA+CGG 0.414472 4:+83602671 None:intergenic
TGAAACCATGTTTAGGATGA+AGG 0.423709 4:-83599322 MsG0480023309.01.T01:intron
CAAACATGGCTTCCCAGTTC+CGG 0.424669 4:-83602704 MsG0480023309.01.T01:five_prime_UTR
TGCCATAGCATCAGCTCGTA+AGG 0.442859 4:-83598907 MsG0480023309.01.T01:CDS
CTAGCTATGAAGTTACATAT+AGG 0.446255 4:-83602938 MsG0480023309.01.T01:five_prime_UTR
TTTAAATGAATTTAACATCA+TGG 0.447072 4:-83601700 MsG0480023309.01.T01:intron
GTTCTGCAAATGCAGCGTTC+AGG 0.447538 4:-83601810 MsG0480023309.01.T01:intron
AATTGTGGCATTTGTGATGA+TGG 0.464048 4:-83602968 MsG0480023309.01.T01:five_prime_UTR
TATCGGTATAATTATTCGGC+TGG 0.483287 4:-83601747 MsG0480023309.01.T01:five_prime_UTR
CTCAAATGCAAATGAGCAGC+TGG 0.484860 4:-83598815 MsG0480023309.01.T01:CDS
TGTGACACAGACACCATTTG+TGG 0.486484 4:+83601545 None:intergenic
TTCTTTGCATTTGTCGGACG+AGG 0.486862 4:-83599522 MsG0480023309.01.T01:intron
GTAATCATGTCACTTGTTCA+AGG 0.489787 4:-83602655 MsG0480023309.01.T01:five_prime_UTR
TAAAGAATTTGCTTTCATGA+AGG 0.495198 4:-83602817 MsG0480023309.01.T01:intron
ATAAATTCTGATAGGGAAGA+TGG 0.495943 4:-83599590 MsG0480023309.01.T01:CDS
GATAAAGGAAGAGACGAAGC+TGG 0.501108 4:-83599841 MsG0480023309.01.T01:CDS
CGATTACGGTTCACAGCCTC+CGG 0.503311 4:+83602685 None:intergenic
TGATATTTCAATACTAATTG+TGG 0.505216 4:-83602983 MsG0480023309.01.T01:intron
TCCCTTACGAGCTGATGCTA+TGG 0.510097 4:+83598905 None:intergenic
GAAGGAAGAAACTTGCATCT+AGG 0.510712 4:-83598884 MsG0480023309.01.T01:CDS
GACATGTCCATTGCTTGAGC+AGG 0.513363 4:-83602734 MsG0480023309.01.T01:intron
TGAAAACAGCGGATCTGAAA+AGG 0.514234 4:-83599263 MsG0480023309.01.T01:CDS
AGTGTGATATAAATTCTGAT+AGG 0.525674 4:-83599598 MsG0480023309.01.T01:CDS
TAGGTCATCTCACAATTCTA+AGG 0.526345 4:-83598838 MsG0480023309.01.T01:CDS
GAGCAGGCTTTGCACAAACA+TGG 0.532199 4:-83602718 MsG0480023309.01.T01:intron
GCAGTTTATTGAAGGTGAGA+TGG 0.533264 4:-83599621 MsG0480023309.01.T01:intron
TGCAAAGCCTGCTCAAGCAA+TGG 0.537492 4:+83602727 None:intergenic
TATGAAGTTACATATAGGCT+TGG 0.545659 4:-83602933 MsG0480023309.01.T01:five_prime_UTR
GTGTGATATAAATTCTGATA+GGG 0.552531 4:-83599597 MsG0480023309.01.T01:CDS
GATACAGAACTTGCTTCTAG+TGG 0.559227 4:-83599775 MsG0480023309.01.T01:CDS
TCTTCAATAGACATTAGCAC+TGG 0.564830 4:-83599805 MsG0480023309.01.T01:CDS
GGTTCACAGCCTCCGGAACT+GGG 0.574058 4:+83602692 None:intergenic
GGAAAACAAAGATAATGCAG+TGG 0.574759 4:-83598976 MsG0480023309.01.T01:CDS
CAGGTACTTTGATCGTGATG+TGG 0.577248 4:-83600881 MsG0480023309.01.T01:CDS
GAAAACAAAGATAATGCAGT+GGG 0.582519 4:-83598975 MsG0480023309.01.T01:CDS
ATTATTCGGCTGGCAGAACA+TGG 0.588090 4:-83601737 MsG0480023309.01.T01:five_prime_UTR
AAGATGAGGAAGATATTCAG+AGG 0.596089 4:-83599741 MsG0480023309.01.T01:CDS
TAGCATCAGCTCGTAAGGGA+AGG 0.607120 4:-83598902 MsG0480023309.01.T01:CDS
TGAAAGAAGGGCACTTACAG+AGG 0.613053 4:+83602628 None:intergenic
CAAATTCTTTAAGTGCAGCA+AGG 0.616233 4:+83602829 None:intergenic
AGATGAGGAAGATATTCAGA+GGG 0.621735 4:-83599740 MsG0480023309.01.T01:intron
GCCATAGCATCAGCTCGTAA+GGG 0.630512 4:-83598906 MsG0480023309.01.T01:CDS
ACATGGCTTCCCAGTTCCGG+AGG 0.633971 4:-83602701 MsG0480023309.01.T01:five_prime_UTR
CAAACAGCTTGAGTAAGCAA+AGG 0.642212 4:-83598935 MsG0480023309.01.T01:CDS
AAACAGCGGATCTGAAAAGG+AGG 0.665431 4:-83599260 MsG0480023309.01.T01:CDS
TTTGAAGCAGGTGAAAACAG+CGG 0.730668 4:-83599274 MsG0480023309.01.T01:CDS
CATTTGTGGAAAATCAACCA+CGG 0.740102 4:+83601559 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GAAAAAAAAAAAAAAATCAT+CGG + Chr4:83602872-83602891 None:intergenic 10.0%
!! AAAAATAAACAGAATTCAAA+GGG + Chr4:83600804-83600823 None:intergenic 15.0%
!! GAAATATGTATATTATTCAA+TGG - Chr4:83601747-83601766 MsG0480023309.01.T01:five_prime_UTR 15.0%
!! TTTAAATGAATTTAACATCA+TGG - Chr4:83600572-83600591 MsG0480023309.01.T01:intron 15.0%
!!! AAAATTCTATCGATTTTATT+AGG - Chr4:83603053-83603072 MsG0480023309.01.T01:intron 15.0%
!!! ATAATATAGATTTTGAATTG+TGG - Chr4:83600133-83600152 MsG0480023309.01.T01:intron 15.0%
!!! ATCTAAAACTAAACAATATA+GGG + Chr4:83600094-83600113 None:intergenic 15.0%
!!! TATCTAAAACTAAACAATAT+AGG + Chr4:83600095-83600114 None:intergenic 15.0%
!!! TTTCTTTTATGATTATATTG+TGG - Chr4:83599962-83599981 MsG0480023309.01.T01:intron 15.0%
!! AACATCAAAGTAGTTATAAT+TGG - Chr4:83599406-83599425 MsG0480023309.01.T01:intron 20.0%
!! AAGTTCAAATGAAAATACAA+TGG - Chr4:83603138-83603157 MsG0480023309.01.T01:intron 20.0%
!! AATAACTATTACTATTGAGT+AGG + Chr4:83601857-83601876 None:intergenic 20.0%
!! AATTCGTAGTAGAATATAAA+AGG + Chr4:83601106-83601125 None:intergenic 20.0%
!! AGTTCAAATGAAAATACAAT+GGG - Chr4:83603139-83603158 MsG0480023309.01.T01:intron 20.0%
!! CAAATGTTATACAATTATGA+CGG + Chr4:83599801-83599820 None:intergenic 20.0%
!! CAATTATCGGTATAATTATT+CGG - Chr4:83600521-83600540 MsG0480023309.01.T01:intron 20.0%
!! CATTTACTCAAAATTATTAG+TGG - Chr4:83599234-83599253 MsG0480023309.01.T01:intron 20.0%
!! GAAAAATAAACAGAATTCAA+AGG + Chr4:83600805-83600824 None:intergenic 20.0%
!! GAAACAAAATAATGAAAGAA+GGG + Chr4:83599659-83599678 None:intergenic 20.0%
!! TACGCATATTAAATAAAAAG+CGG + Chr4:83602226-83602245 None:intergenic 20.0%
!! TGAAACAAAATAATGAAAGA+AGG + Chr4:83599660-83599679 None:intergenic 20.0%
!! TGATATTTCAATACTAATTG+TGG - Chr4:83599289-83599308 MsG0480023309.01.T01:CDS 20.0%
!!! ATAGTTTTATACCACAAAAA+TGG + Chr4:83601074-83601093 None:intergenic 20.0%
!!! CTTAACTTATTGAAAGATTT+TGG - Chr4:83602302-83602321 MsG0480023309.01.T01:intron 20.0%
!!! GATTTCATTTATTTTCACTA+CGG + Chr4:83601218-83601237 None:intergenic 20.0%
!!! TAGTTTTATACCACAAAAAT+GGG + Chr4:83601073-83601092 None:intergenic 20.0%
! AAAATGGGAAGATAAATCTA+TGG + Chr4:83601058-83601077 None:intergenic 25.0%
! AATTAAAAGCAAGGATCAAA+TGG + Chr4:83599873-83599892 None:intergenic 25.0%
! AGTGTGATATAAATTCTGAT+AGG - Chr4:83602674-83602693 MsG0480023309.01.T01:five_prime_UTR 25.0%
! ATCAAAACCATGTTTAACAA+AGG - Chr4:83599068-83599087 MsG0480023309.01.T01:intron 25.0%
! ATTGCTTGAATTTGTATACT+TGG - Chr4:83600440-83600459 MsG0480023309.01.T01:intron 25.0%
! CAAATCAAATTACACCATTA+TGG + Chr4:83603271-83603290 None:intergenic 25.0%
! CAAGGAAAAATAATCAGTTA+AGG + Chr4:83601368-83601387 None:intergenic 25.0%
! CATATAAAGGCTAGATAAAA+AGG + Chr4:83603244-83603263 None:intergenic 25.0%
! CCTTTATATGAAAACCATAA+TGG - Chr4:83603254-83603273 MsG0480023309.01.T01:five_prime_UTR 25.0%
! GAAACAAAATATAGCAAATG+AGG + Chr4:83600885-83600904 None:intergenic 25.0%
! GTATAAGATCTATATGTCAT+TGG - Chr4:83600959-83600978 MsG0480023309.01.T01:intron 25.0%
! GTGTGATATAAATTCTGATA+GGG - Chr4:83602675-83602694 MsG0480023309.01.T01:five_prime_UTR 25.0%
! TCATATTTAACATATGACTG+TGG - Chr4:83601513-83601532 MsG0480023309.01.T01:intron 25.0%
! TGTAACATTTCCATTACTAT+TGG + Chr4:83601252-83601271 None:intergenic 25.0%
! TTAAAGCTTACCAATAGTAA+TGG - Chr4:83601239-83601258 MsG0480023309.01.T01:intron 25.0%
! TTCAATCATAAAAGTCGTTT+AGG - Chr4:83599676-83599695 MsG0480023309.01.T01:intron 25.0%
! TTGTTAATGAAACCATGTTT+AGG - Chr4:83602943-83602962 MsG0480023309.01.T01:five_prime_UTR 25.0%
!! AACATTTCAAGTATATAGCA+AGG + Chr4:83599494-83599513 None:intergenic 25.0%
!! ACATTTCAAGTATATAGCAA+GGG + Chr4:83599493-83599512 None:intergenic 25.0%
!! CCATTATGGTTTTCATATAA+AGG + Chr4:83603257-83603276 None:intergenic 25.0%
!! CTTTTATGATTATATTGTGG+TGG - Chr4:83599965-83599984 MsG0480023309.01.T01:intron 25.0%
!! TAAAGAATTTGCTTTCATGA+AGG - Chr4:83599455-83599474 MsG0480023309.01.T01:intron 25.0%
!! TAGATTTTGAATTGTGGTTA+TGG - Chr4:83600139-83600158 MsG0480023309.01.T01:intron 25.0%
!!! ACAGTTTTTGAGACAATTAT+CGG - Chr4:83600508-83600527 MsG0480023309.01.T01:intron 25.0%
!!! ACTAGTTCTGAAAAACAAAA+AGG + Chr4:83600025-83600044 None:intergenic 25.0%
!!! AGATGACTTTTTCTCTAATA+AGG - Chr4:83602843-83602862 MsG0480023309.01.T01:five_prime_UTR 25.0%
!!! ATAACATTTGATTTGATCTG+TGG - Chr4:83599811-83599830 MsG0480023309.01.T01:CDS 25.0%
!!! ATGCCACAATTTTTAATTCT+AGG - Chr4:83599998-83600017 MsG0480023309.01.T01:intron 25.0%
!!! TCTTTAAGTTTTTCAGAAAG+AGG - Chr4:83601420-83601439 MsG0480023309.01.T01:intron 25.0%
!!! TGATTATTTTTCCTTGTTTC+AGG - Chr4:83601372-83601391 MsG0480023309.01.T01:intron 25.0%
!!! TGTTTAGTTTTAGATAACGA+AGG - Chr4:83600099-83600118 MsG0480023309.01.T01:intron 25.0%
!!! TTTGTTAAACATGGTTTTGA+TGG + Chr4:83599069-83599088 None:intergenic 25.0%
AAAGACACCTTTGTTAAACA+TGG + Chr4:83599078-83599097 None:intergenic 30.0%
AATGATCATCATGTCAATGT+AGG + Chr4:83599184-83599203 None:intergenic 30.0%
AGAGAAAAAGTCATCTCTAT+TGG + Chr4:83602839-83602858 None:intergenic 30.0%
AGGAATTCTCAAAAAGACAA+GGG - Chr4:83603408-83603427 MsG0480023309.01.T01:five_prime_UTR 30.0%
AGTAGTATGTCTTAACTGTA+TGG - Chr4:83601271-83601290 MsG0480023309.01.T01:intron 30.0%
AGTGTAAAATATGTGGAAGT+GGG + Chr4:83600406-83600425 None:intergenic 30.0%
ATAAATTCTGATAGGGAAGA+TGG - Chr4:83602682-83602701 MsG0480023309.01.T01:five_prime_UTR 30.0%
ATCTATCTGCAGTTTATTGA+AGG - Chr4:83602643-83602662 MsG0480023309.01.T01:intron 30.0%
ATGAACACAATTAGTCAACT+GGG - Chr4:83599719-83599738 MsG0480023309.01.T01:intron 30.0%
ATTGTTGTGTACACAATCAT+TGG + Chr4:83602200-83602219 None:intergenic 30.0%
CAAAAGCAGTGTAAAATATG+TGG + Chr4:83600413-83600432 None:intergenic 30.0%
CATCATATTTAGAAGAGTCA+TGG + Chr4:83599010-83599029 None:intergenic 30.0%
CATGACTCTTCTAAATATGA+TGG - Chr4:83599008-83599027 MsG0480023309.01.T01:intron 30.0%
CGAGATGTAAATTAAAAGCA+AGG + Chr4:83599882-83599901 None:intergenic 30.0%
CTAGCTATGAAGTTACATAT+AGG - Chr4:83599334-83599353 MsG0480023309.01.T01:intron 30.0%
CTATTACTATTGAGTAGGTT+AGG + Chr4:83601852-83601871 None:intergenic 30.0%
CTGTTTGTGATTTCTATCAA+TGG + Chr4:83601721-83601740 None:intergenic 30.0%
CTTAACTGTATGGTATCAAT+AGG - Chr4:83601281-83601300 MsG0480023309.01.T01:intron 30.0%
GAAAACAAAGATAATGCAGT+GGG - Chr4:83603297-83603316 MsG0480023309.01.T01:five_prime_UTR 30.0%
GAACATCTTGAAATCAAAGA+TGG + Chr4:83601324-83601343 None:intergenic 30.0%
GAGAACCTTAAAATCATCTT+TGG + Chr4:83598859-83598878 None:intergenic 30.0%
GGACCTAGAATTAAAAATTG+TGG + Chr4:83600004-83600023 None:intergenic 30.0%
GTTAGTGCTTTGTAGAATAT+CGG - Chr4:83599266-83599285 MsG0480023309.01.T01:CDS 30.0%
TACAGCATTCCTTGTTAATA+AGG + Chr4:83603109-83603128 None:intergenic 30.0%
TAGGAATTCTCAAAAAGACA+AGG - Chr4:83603407-83603426 MsG0480023309.01.T01:five_prime_UTR 30.0%
TATATTAAAACATGCAGCTG+AGG + Chr4:83599152-83599171 None:intergenic 30.0%
TATGAAGTTACATATAGGCT+TGG - Chr4:83599339-83599358 MsG0480023309.01.T01:intron 30.0%
TTCATTTCAGAATTGTCATG+AGG + Chr4:83600862-83600881 None:intergenic 30.0%
TTGTTGTGTACACAATCATT+GGG + Chr4:83602199-83602218 None:intergenic 30.0%
TTTATGACAACAAAAGCTGA+TGG + Chr4:83602784-83602803 None:intergenic 30.0%
! ATTTTCAGAGATGATGCATA+GGG + Chr4:83599926-83599945 None:intergenic 30.0%
! CACATATTTTACACTGCTTT+TGG - Chr4:83600411-83600430 MsG0480023309.01.T01:intron 30.0%
! TAAGTCTTTTGTTCTTAAGC+TGG - Chr4:83600343-83600362 MsG0480023309.01.T01:intron 30.0%
! TATTTTCAGAGATGATGCAT+AGG + Chr4:83599927-83599946 None:intergenic 30.0%
! TGATTACACAATGTCGATTA+CGG + Chr4:83599604-83599623 None:intergenic 30.0%
!! AATGGTGTAATTTGATTTGC+AGG - Chr4:83603272-83603291 MsG0480023309.01.T01:five_prime_UTR 30.0%
!! AGTTAGCAAAGATAGTTTTG+TGG + Chr4:83600062-83600081 None:intergenic 30.0%
!! CAAGTTTTTCAAAACCTTGT+AGG + Chr4:83598967-83598986 None:intergenic 30.0%
!! GATTTATCTTCCCATTTTTG+TGG - Chr4:83601060-83601079 MsG0480023309.01.T01:intron 30.0%
!! TGGCTTTAGTAAAAAAGATG+AGG - Chr4:83602517-83602536 MsG0480023309.01.T01:intron 30.0%
!! TGTTGATTCTTTGCATTTGT+CGG - Chr4:83602744-83602763 MsG0480023309.01.T01:intron 30.0%
!! TTTATTTTCACTACGGCATA+CGG + Chr4:83601211-83601230 None:intergenic 30.0%
AAGATGAGGAAGATATTCAG+AGG - Chr4:83602531-83602550 MsG0480023309.01.T01:intron 35.0%
AAGGGACGTAATAGCATATA+AGG + Chr4:83600786-83600805 None:intergenic 35.0%
AATTCTCAAAAAGACAAGGG+TGG - Chr4:83603411-83603430 MsG0480023309.01.T01:five_prime_UTR 35.0%
AATTGTGGCATTTGTGATGA+TGG - Chr4:83599304-83599323 MsG0480023309.01.T01:CDS 35.0%
AGATGAGGAAGATATTCAGA+GGG - Chr4:83602532-83602551 MsG0480023309.01.T01:intron 35.0%
AGCATTCCTTGTTAATAAGG+TGG + Chr4:83603106-83603125 None:intergenic 35.0%
AGCTCTTTACTTGAAGGATA+AGG + Chr4:83601688-83601707 None:intergenic 35.0%
AGGTTTACACAAGAAGAAAG+AGG + Chr4:83602148-83602167 None:intergenic 35.0%
ATTCCTTGTTAATAAGGTGG+AGG + Chr4:83603103-83603122 None:intergenic 35.0%
ATTGTCTCAAAAACTGTCTC+TGG + Chr4:83600505-83600524 None:intergenic 35.0%
CAAATTCTTTAAGTGCAGCA+AGG + Chr4:83599446-83599465 None:intergenic 35.0%
CAGTGTAAAATATGTGGAAG+TGG + Chr4:83600407-83600426 None:intergenic 35.0%
CATAGAAGCTCTACAATGAA+GGG - Chr4:83601780-83601799 MsG0480023309.01.T01:five_prime_UTR 35.0%
CATGAACACAATTAGTCAAC+TGG - Chr4:83599718-83599737 MsG0480023309.01.T01:intron 35.0%
CATTTGTGGAAAATCAACCA+CGG + Chr4:83600716-83600735 None:intergenic 35.0%
GAGTTTGATGCATGATATCA+AGG - Chr4:83601625-83601644 MsG0480023309.01.T01:intron 35.0%
GATAAATGTTTCACGATCGA+CGG + Chr4:83602592-83602611 None:intergenic 35.0%
GATAACGACGATTCTGATAA+AGG - Chr4:83602416-83602435 MsG0480023309.01.T01:intron 35.0%
GCTCTTTACTTGAAGGATAA+GGG + Chr4:83601687-83601706 None:intergenic 35.0%
GGAAAACAAAGATAATGCAG+TGG - Chr4:83603296-83603315 MsG0480023309.01.T01:five_prime_UTR 35.0%
GGTTTACACAAGAAGAAAGA+GGG + Chr4:83602147-83602166 None:intergenic 35.0%
TAGGTCATCTCACAATTCTA+AGG - Chr4:83603434-83603453 MsG0480023309.01.T01:five_prime_UTR 35.0%
TATAGAGTTCATGAAGCTGT+AGG + Chr4:83602023-83602042 None:intergenic 35.0%
TATCGGTATAATTATTCGGC+TGG - Chr4:83600525-83600544 MsG0480023309.01.T01:intron 35.0%
TCATCATGTCAATGTAGGTA+CGG + Chr4:83599179-83599198 None:intergenic 35.0%
TCTTCAATAGACATTAGCAC+TGG - Chr4:83602467-83602486 MsG0480023309.01.T01:intron 35.0%
TGAACCTAAAGCACAAAAGA+GGG + Chr4:83602370-83602389 None:intergenic 35.0%
TGGGAAGATAAATCTATGGA+CGG + Chr4:83601054-83601073 None:intergenic 35.0%
TGTTGTGTACACAATCATTG+GGG + Chr4:83602198-83602217 None:intergenic 35.0%
TTAAATCGTCTGCTTCATTC+AGG + Chr4:83602723-83602742 None:intergenic 35.0%
TTGAACCTAAAGCACAAAAG+AGG + Chr4:83602371-83602390 None:intergenic 35.0%
! AAGATTTTGGATCTTGTCTG+TGG - Chr4:83602315-83602334 MsG0480023309.01.T01:intron 35.0%
! ATTTCTATCAATGGCCTTAG+AGG + Chr4:83601712-83601731 None:intergenic 35.0%
! CCTCTCCAAAGATGATTTTA+AGG - Chr4:83598851-83598870 MsG0480023309.01.T01:CDS 35.0%
! CCTTAAAATCATCTTTGGAG+AGG + Chr4:83598854-83598873 None:intergenic 35.0%
! GTAATCATGTCACTTGTTCA+AGG - Chr4:83599617-83599636 MsG0480023309.01.T01:CDS 35.0%
! TGAAACCATGTTTAGGATGA+AGG - Chr4:83602950-83602969 MsG0480023309.01.T01:five_prime_UTR 35.0%
! TGGTTTGTGAACTACTCTTA+GGG - Chr4:83599758-83599777 MsG0480023309.01.T01:CDS 35.0%
! TTATTTTCACTACGGCATAC+GGG + Chr4:83601210-83601229 None:intergenic 35.0%
! TTCTAAAAAGCTAGCTCAAG+TGG + Chr4:83601602-83601621 None:intergenic 35.0%
!! AAAAAAGAGAGCTTTGAAGC+AGG - Chr4:83602986-83603005 MsG0480023309.01.T01:intron 35.0%
!! AAAGGTGTCTTTGTTTCTGT+TGG - Chr4:83599086-83599105 MsG0480023309.01.T01:intron 35.0%
!! ATGTTTTAAGTAGTCACGCT+TGG + Chr4:83599391-83599410 None:intergenic 35.0%
!!! TTGTTCTTAAGCTGGCTTTT+TGG - Chr4:83600351-83600370 MsG0480023309.01.T01:intron 35.0%
AAATATGTGGAAGTGGGTAG+TGG + Chr4:83600400-83600419 None:intergenic 40.0%
ACACAATTAGTCAACTGGGA+CGG - Chr4:83599723-83599742 MsG0480023309.01.T01:intron 40.0%
ACATATGACCAACTTCTGGA+AGG + Chr4:83602168-83602187 None:intergenic 40.0%
AGAACAAAACCAGAAGACCA+TGG + Chr4:83601651-83601670 None:intergenic 40.0%
AGAGGAAGCTCTTTACTTGA+AGG + Chr4:83601694-83601713 None:intergenic 40.0%
AGGAAGATATTCAGAGGGTA+TGG - Chr4:83602537-83602556 MsG0480023309.01.T01:intron 40.0%
CAAAAGAGGGACTAACTATG+AGG + Chr4:83602357-83602376 None:intergenic 40.0%
CAAACAGCTTGAGTAAGCAA+AGG - Chr4:83603337-83603356 MsG0480023309.01.T01:five_prime_UTR 40.0%
CAAGTAAAGAGCTTCCTCTA+AGG - Chr4:83601695-83601714 MsG0480023309.01.T01:intron 40.0%
CAAGTAAGCACAGGTTATAC+AGG + Chr4:83600602-83600621 None:intergenic 40.0%
CATGTCCTTCATCCTAAACA+TGG + Chr4:83602958-83602977 None:intergenic 40.0%
CGATGAAGAAAAAGTTACCG+TGG - Chr4:83600696-83600715 MsG0480023309.01.T01:intron 40.0%
CTTCCTCCACCTTATTAACA+AGG - Chr4:83603097-83603116 MsG0480023309.01.T01:intron 40.0%
GAAGGAAGAAACTTGCATCT+AGG - Chr4:83603388-83603407 MsG0480023309.01.T01:five_prime_UTR 40.0%
GAGAAAATCGTAACCAAGGT+AGG + Chr4:83599045-83599064 None:intergenic 40.0%
GAGAGAATATCTCTCTGGTT+AGG + Chr4:83601830-83601849 None:intergenic 40.0%
GCAGTTTATTGAAGGTGAGA+TGG - Chr4:83602651-83602670 MsG0480023309.01.T01:five_prime_UTR 40.0%
GCATAGAAGCTCTACAATGA+AGG - Chr4:83601779-83601798 MsG0480023309.01.T01:five_prime_UTR 40.0%
GCTAACATATTCTCTCCTCT+TGG - Chr4:83602116-83602135 MsG0480023309.01.T01:intron 40.0%
GTTAGGAGAGAATATCTCTC+TGG + Chr4:83601835-83601854 None:intergenic 40.0%
TAATGCTGACAAGTAAGCAC+AGG + Chr4:83600611-83600630 None:intergenic 40.0%
TACACAAGAAGAAAGAGGGT+GGG + Chr4:83602143-83602162 None:intergenic 40.0%
TGAAAACAGCGGATCTGAAA+AGG - Chr4:83603009-83603028 MsG0480023309.01.T01:intron 40.0%
TGTGTAAACCTTCCAGAAGT+TGG - Chr4:83602157-83602176 MsG0480023309.01.T01:intron 40.0%
TGTTTGTGAATCTCAGTGCT+TGG - Chr4:83601472-83601491 MsG0480023309.01.T01:intron 40.0%
TTAAAACATGCAGCTGAGGT+AGG + Chr4:83599148-83599167 None:intergenic 40.0%
TTACACAAGAAGAAAGAGGG+TGG + Chr4:83602144-83602163 None:intergenic 40.0%
TTGTGAACTACTCTTAGGGA+TGG - Chr4:83599762-83599781 MsG0480023309.01.T01:CDS 40.0%
! AAGATATTCAGAGGGTATGG+TGG - Chr4:83602540-83602559 MsG0480023309.01.T01:intron 40.0%
! ATTCTTTTCCAACACCGATC+TGG + Chr4:83599115-83599134 None:intergenic 40.0%
! CGATCAAAGTACCTGAAACA+AGG + Chr4:83601386-83601405 None:intergenic 40.0%
! GATACAGAACTTGCTTCTAG+TGG - Chr4:83602497-83602516 MsG0480023309.01.T01:intron 40.0%
! GTGGTTTGTGAACTACTCTT+AGG - Chr4:83599757-83599776 MsG0480023309.01.T01:CDS 40.0%
! TAGTCCCTCTTTTGTGCTTT+AGG - Chr4:83602363-83602382 MsG0480023309.01.T01:intron 40.0%
! TTCCAGAAGTTGGTCATATG+TGG - Chr4:83602167-83602186 MsG0480023309.01.T01:intron 40.0%
! TTTGAAGCAGGTGAAAACAG+CGG - Chr4:83602998-83603017 MsG0480023309.01.T01:intron 40.0%
!! ATCGGTGTTGGAAAAGAATC+TGG - Chr4:83599116-83599135 MsG0480023309.01.T01:intron 40.0%
!! CGTGGTTGATTTTCCACAAA+TGG - Chr4:83600714-83600733 MsG0480023309.01.T01:intron 40.0%
!! GACGGATTACAACTAAGAGA+AGG + Chr4:83602574-83602593 None:intergenic 40.0%
!! GATAGATTTGAAGAGCGTTG+AGG + Chr4:83602630-83602649 None:intergenic 40.0%
!! GGTGTAATTTGATTTGCAGG+AGG - Chr4:83603275-83603294 MsG0480023309.01.T01:five_prime_UTR 40.0%
!!! CTTAAGCTGGCTTTTTGGTT+TGG - Chr4:83600356-83600375 MsG0480023309.01.T01:intron 40.0%
!!! TGATTTTAAGGTTCTCTCCG+CGG - Chr4:83598863-83598882 MsG0480023309.01.T01:CDS 40.0%
AAACAGCGGATCTGAAAAGG+AGG - Chr4:83603012-83603031 MsG0480023309.01.T01:intron 45.0%
AATCGTAACCAAGGTAGGTG+AGG + Chr4:83599040-83599059 None:intergenic 45.0%
ACACAAGAAGAAAGAGGGTG+GGG + Chr4:83602142-83602161 None:intergenic 45.0%
ATATCAAGGCCATGGTCTTC+TGG - Chr4:83601639-83601658 MsG0480023309.01.T01:intron 45.0%
ATTATTCGGCTGGCAGAACA+TGG - Chr4:83600535-83600554 MsG0480023309.01.T01:intron 45.0%
ATTCGGACAATGAGTTCAGC+AGG + Chr4:83598921-83598940 None:intergenic 45.0%
CAGGTACTTTGATCGTGATG+TGG - Chr4:83601391-83601410 MsG0480023309.01.T01:intron 45.0%
CATGCTTGAGACAAGCGATT+CGG + Chr4:83598938-83598957 None:intergenic 45.0%
CTCAAAAAGACAAGGGTGGT+AGG - Chr4:83603415-83603434 MsG0480023309.01.T01:five_prime_UTR 45.0%
CTCAAATGCAAATGAGCAGC+TGG - Chr4:83603457-83603476 MsG0480023309.01.T01:five_prime_UTR 45.0%
GATAAAGGAAGAGACGAAGC+TGG - Chr4:83602431-83602450 MsG0480023309.01.T01:intron 45.0%
GATGCATGATATCAAGGCCA+TGG - Chr4:83601631-83601650 MsG0480023309.01.T01:intron 45.0%
GGACTAACTATGAGGACAAC+TGG + Chr4:83602349-83602368 None:intergenic 45.0%
GTCCACATATGACCAACTTC+TGG + Chr4:83602172-83602191 None:intergenic 45.0%
GTTCAGCAGGTACAATGTCA+TGG + Chr4:83598908-83598927 None:intergenic 45.0%
TGGCGAGAAAATCGTAACCA+AGG + Chr4:83599049-83599068 None:intergenic 45.0%
TGTGACACAGACACCATTTG+TGG + Chr4:83600730-83600749 None:intergenic 45.0%
TTCTTTGCATTTGTCGGACG+AGG - Chr4:83602750-83602769 MsG0480023309.01.T01:intron 45.0%
! AGTGCTTGGTTTGAGGAGAA+TGG - Chr4:83601486-83601505 MsG0480023309.01.T01:intron 45.0%
! GAATCTCAGTGCTTGGTTTG+AGG - Chr4:83601479-83601498 MsG0480023309.01.T01:intron 45.0%
! GTGCTTGGTTTGAGGAGAAT+GGG - Chr4:83601487-83601506 MsG0480023309.01.T01:intron 45.0%
! TGCTTGGTTTGAGGAGAATG+GGG - Chr4:83601488-83601507 MsG0480023309.01.T01:intron 45.0%
! TGGGACGGTGTTAGATATTG+TGG - Chr4:83599738-83599757 MsG0480023309.01.T01:intron 45.0%
!! TGAAAGAAGGGCACTTACAG+AGG + Chr4:83599647-83599666 None:intergenic 45.0%
!! TATATTTCATAATTTAATTA+GGG + Chr4:83600221-83600240 None:intergenic 5.0%
!! TTATATTTCATAATTTAATT+AGG + Chr4:83600222-83600241 None:intergenic 5.0%
ATCGCTTGTCTCAAGCATGG+TGG - Chr4:83598939-83598958 MsG0480023309.01.T01:CDS 50.0%
CAAACATGGCTTCCCAGTTC+CGG - Chr4:83599568-83599587 MsG0480023309.01.T01:CDS 50.0%
CGAATCGCTTGTCTCAAGCA+TGG - Chr4:83598936-83598955 MsG0480023309.01.T01:CDS 50.0%
GACATGTCCATTGCTTGAGC+AGG - Chr4:83599538-83599557 MsG0480023309.01.T01:CDS 50.0%
GAGCAGGCTTTGCACAAACA+TGG - Chr4:83599554-83599573 MsG0480023309.01.T01:CDS 50.0%
GCCATAGCATCAGCTCGTAA+GGG - Chr4:83603366-83603385 MsG0480023309.01.T01:five_prime_UTR 50.0%
GTTCTGCAAATGCAGCGTTC+AGG - Chr4:83600462-83600481 MsG0480023309.01.T01:intron 50.0%
GTTTCTGTTGGTCGCCAGAT+CGG - Chr4:83599098-83599117 MsG0480023309.01.T01:intron 50.0%
TAGCATCAGCTCGTAAGGGA+AGG - Chr4:83603370-83603389 MsG0480023309.01.T01:five_prime_UTR 50.0%
TCCCTTACGAGCTGATGCTA+TGG + Chr4:83603370-83603389 None:intergenic 50.0%
TGCAAAGCCTGCTCAAGCAA+TGG + Chr4:83599548-83599567 None:intergenic 50.0%
TGCAAATGAGCAGCTGGTGA+AGG - Chr4:83603463-83603482 MsG0480023309.01.T01:five_prime_UTR 50.0%
TGCCATAGCATCAGCTCGTA+AGG - Chr4:83603365-83603384 MsG0480023309.01.T01:five_prime_UTR 50.0%
TGGTTAGGAGTCTCTGACTC+TGG + Chr4:83601815-83601834 None:intergenic 50.0%
AGGTTCTCTCCGCGGTTGAA+TGG - Chr4:83598871-83598890 MsG0480023309.01.T01:CDS 55.0%
CAAGCATGGTGGCACCTACA+AGG - Chr4:83598950-83598969 MsG0480023309.01.T01:CDS 55.0%
CGATTACGGTTCACAGCCTC+CGG + Chr4:83599590-83599609 None:intergenic 55.0%
GGTTCTCTCCGCGGTTGAAT+GGG - Chr4:83598872-83598891 MsG0480023309.01.T01:CDS 55.0%
GTTCTCTCCGCGGTTGAATG+GGG - Chr4:83598873-83598892 MsG0480023309.01.T01:CDS 55.0%
TCTCATGCCCCATTCAACCG+CGG + Chr4:83598883-83598902 None:intergenic 55.0%
ACATGGCTTCCCAGTTCCGG+AGG - Chr4:83599571-83599590 MsG0480023309.01.T01:CDS 60.0%
AGAAAGAGGGTGGGGCCAAG+AGG + Chr4:83602134-83602153 None:intergenic 60.0%
GGCTTTCGCCTCACCTACCT+TGG - Chr4:83599029-83599048 MsG0480023309.01.T01:intron 60.0%
GGTTCACAGCCTCCGGAACT+GGG + Chr4:83599583-83599602 None:intergenic 60.0%
GTTGGTCGCCAGATCGGTGT+TGG - Chr4:83599104-83599123 MsG0480023309.01.T01:intron 60.0%
CGGTTCACAGCCTCCGGAAC+TGG + Chr4:83599584-83599603 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
Chr4 gene 83598812 83603482 83598812 ID=MsG0480023309.01;Name=MsG0480023309.01
Chr4 mRNA 83598812 83603482 83598812 ID=MsG0480023309.01.T01;Parent=MsG0480023309.01;Name=MsG0480023309.01.T01;_AED=0.17;_eAED=0.17;_QI=707|0.66|0.6|1|0.44|0.6|10|0|226
Chr4 exon 83598812 83599000 83598812 ID=MsG0480023309.01.T01:exon:5519;Parent=MsG0480023309.01.T01
Chr4 exon 83599249 83599329 83599249 ID=MsG0480023309.01.T01:exon:5518;Parent=MsG0480023309.01.T01
Chr4 exon 83599523 83599639 83599523 ID=MsG0480023309.01.T01:exon:5517;Parent=MsG0480023309.01.T01
Chr4 exon 83599741 83599909 83599741 ID=MsG0480023309.01.T01:exon:5516;Parent=MsG0480023309.01.T01
Chr4 exon 83600853 83600900 83600853 ID=MsG0480023309.01.T01:exon:5515;Parent=MsG0480023309.01.T01
Chr4 exon 83601530 83601603 83601530 ID=MsG0480023309.01.T01:exon:5514;Parent=MsG0480023309.01.T01
Chr4 exon 83601701 83601817 83601701 ID=MsG0480023309.01.T01:exon:5513;Parent=MsG0480023309.01.T01
Chr4 exon 83602646 83602734 83602646 ID=MsG0480023309.01.T01:exon:5512;Parent=MsG0480023309.01.T01
Chr4 exon 83602818 83602995 83602818 ID=MsG0480023309.01.T01:exon:5511;Parent=MsG0480023309.01.T01
Chr4 five_prime_UTR 83603157 83603482 83603157 ID=MsG0480023309.01.T01:five_prime_utr;Parent=MsG0480023309.01.T01
Chr4 five_prime_UTR 83602818 83602995 83602818 ID=MsG0480023309.01.T01:five_prime_utr;Parent=MsG0480023309.01.T01
Chr4 five_prime_UTR 83602646 83602734 83602646 ID=MsG0480023309.01.T01:five_prime_utr;Parent=MsG0480023309.01.T01
Chr4 five_prime_UTR 83601704 83601817 83601704 ID=MsG0480023309.01.T01:five_prime_utr;Parent=MsG0480023309.01.T01
Chr4 CDS 83601701 83601703 83601701 ID=MsG0480023309.01.T01:cds;Parent=MsG0480023309.01.T01
Chr4 CDS 83601530 83601603 83601530 ID=MsG0480023309.01.T01:cds;Parent=MsG0480023309.01.T01
Chr4 CDS 83600853 83600900 83600853 ID=MsG0480023309.01.T01:cds;Parent=MsG0480023309.01.T01
Chr4 CDS 83599741 83599909 83599741 ID=MsG0480023309.01.T01:cds;Parent=MsG0480023309.01.T01
Chr4 CDS 83599523 83599639 83599523 ID=MsG0480023309.01.T01:cds;Parent=MsG0480023309.01.T01
Chr4 CDS 83599249 83599329 83599249 ID=MsG0480023309.01.T01:cds;Parent=MsG0480023309.01.T01
Chr4 CDS 83598812 83599000 83598812 ID=MsG0480023309.01.T01:cds;Parent=MsG0480023309.01.T01
Chr4 mRNA 83602642 83603482 83602642 ID=MsG0480023309.01.T02;Parent=MsG0480023309.01;Name=MsG0480023309.01.T02;_AED=0.43;_eAED=0.43;_QI=10|0|0.5|1|1|1|2|0|143
Chr4 exon 83602642 83602757 83602642 ID=MsG0480023309.01.T02:exon:5520;Parent=MsG0480023309.01.T02
Chr4 five_prime_UTR 83603473 83603482 83603473 ID=MsG0480023309.01.T02:five_prime_utr;Parent=MsG0480023309.01.T02
Chr4 CDS 83603157 83603472 83603157 ID=MsG0480023309.01.T02:cds;Parent=MsG0480023309.01.T02
Chr4 CDS 83602642 83602757 83602642 ID=MsG0480023309.01.T02:cds;Parent=MsG0480023309.01.T02
Gene Sequence

>MsG0480023309.01.T01

ATGATCGACGATGAAGAAAAAGTTACCGTGGTTGATTTTCCACAAATGGTGTCTGTGTCACACTGTAATGCAAAGATGTACTTTGATCGTGATGTGGAATGTATCTTTAAGTTTTTCAGAAAGAGGTTCAATATTTCTTTTCAAGAAAGAATAGATGATAACGACGATTCTGATAAAGGAAGAGACGAAGCTGGAAAACTCTCTTTTTCTTCAATAGACATTAGCACTGGTTTTCTAGATACAGAACTTGCTTCTAGTGGCTTTAGTAAAAAAGATGAGGAAGATATTCAGAGGTTTATTGAAGGTGAGATGGAGTGTGATATAAATTCTGATAGGGAAGATGGTGACTTAATGAGAAACCTGAATGAAGCAGACGATTTAAATGTTGATTCTTTGCATTTGTCGGACGAGGATGAAGGACATGAAAGTCATAAAAAAGAGAGCTTTGAAGCAGGTGAAAACAGCGGATCTGAAAAGGAGGCAAACAACACTGAGGAAAACAAAGATAATGCAGTGGGAATTGAAGCTGAAATCACAAACAGCTTGAGTAAGCAAAGGAAAAGTGCCATAGCATCAGCTCGTAAGGGAAGGAAGAAACTTGCATCTAGGAATTCTCAAAAAGACAAGGGTGGTAGGTCATCTCACAATTCTAAGGCTCAAATGCAAATGAGCAGCTGGTGA

>MsG0480023309.01.T02

ATGAAGCTCGACGTAGATGTTTTGAGATACCTCTCCAAAGATGATTTTAAGGTTCTCTCCGCGGTTGAATGGGGCATGAGAAACCATGACATTGTACCTGCTGAACTCATTGTCCGAATCGCTTGTCTCAAGCATGGTGGCACCTACAAGGTTTTGAAAAACTTGCTTAAACATAAGCTCTTGCACCATGACTCTTCTAAATATGATGGCTTTCGCCTCACCTACCTTGGTTACGATTTTCTCGCCATCAAAACCATGTTTAACAAAGGTGTCTTTGTTTCTGTTGGTCGCCAGATCGGTGTTGGAAAAGAATCTGTGACATGTCCATTGCTTGAGCAGGCTTTGCACAAACATGGCTTCCCAGTTCCGGAGGCTGTGAACCGTAATCGACATTGTGTAATCATGTCACTTGTTCAAGGTTATCCTCTGTAA
Protein sequence

>MsG0480023309.01.T01

MIDDEEKVTVVDFPQMVSVSHCNAKMYFDRDVECIFKFFRKRFNISFQERIDDNDDSDKGRDEAGKLSFSSIDISTGFLDTELASSGFSKKDEEDIQRFIEGEMECDINSDREDGDLMRNLNEADDLNVDSLHLSDEDEGHESHKKESFEAGENSGSEKEANNTEENKDNAVGIEAEITNSLSKQRKSAIASARKGRKKLASRNSQKDKGGRSSHNSKAQMQMSSW*

>MsG0480023309.01.T02

MKLDVDVLRYLSKDDFKVLSAVEWGMRNHDIVPAELIVRIACLKHGGTYKVLKNLLKHKLLHHDSSKYDGFRLTYLGYDFLAIKTMFNKGVFVSVGRQIGVGKESVTCPLLEQALHKHGFPVPEAVNRNRHCVIMSLVQGYPL*