Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014607.01.T01 | XP_003600436.2 | 99.537 | 216 | 1 | 0 | 1 | 216 | 1 | 216 | 1.10E-156 | 442 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014607.01.T01 | A7RP64 | 60.476 | 210 | 78 | 1 | 7 | 216 | 34 | 238 | 2.52E-90 | 268 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014607.01.T01 | I3S062 | 99.537 | 216 | 1 | 0 | 1 | 216 | 1 | 216 | 5.25e-157 | 442 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180002214.01 | MsG0380014607.01 | -0.823041 | 1.714413e-53 | 1.459845e-50 |
| MsG0180002426.01 | MsG0380014607.01 | -0.809709 | 1.654893e-50 | 9.786880e-48 |
| MsG0180006227.01 | MsG0380014607.01 | -0.804663 | 1.938660e-49 | 1.005407e-46 |
| MsG0380014607.01 | MsG0480021530.01 | 0.806100 | 9.689941e-50 | 5.214298e-47 |
| MsG0380014607.01 | MsG0780036004.01 | -0.819453 | 1.152281e-52 | 8.871032e-50 |
| MsG0380014607.01 | MsG0780037458.01 | -0.802838 | 4.638632e-49 | 2.295658e-46 |
| MsG0380014607.01 | MsG0780039838.01 | -0.803778 | 2.963616e-49 | 1.502333e-46 |
| MsG0380014607.01 | MsG0780039839.01 | -0.801632 | 8.217048e-49 | 3.943296e-46 |
| MsG0380014607.01 | MsG0780041637.01 | -0.810882 | 9.238718e-51 | 5.635726e-48 |
| MsG0380014607.01 | MsG0880046947.01 | 0.841670 | 4.127997e-58 | 6.109088e-55 |
PPI
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014607.01.T01 | MTR_3g061130 | 99.537 | 216 | 1 | 0 | 1 | 216 | 1 | 216 | 1.33e-160 | 442 |
| MsG0380014607.01.T01 | MTR_5g088410 | 60.930 | 215 | 51 | 5 | 3 | 216 | 6 | 188 | 1.13e-75 | 226 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014607.01.T01 | AT3G13230 | 75.472 | 212 | 52 | 0 | 3 | 214 | 4 | 215 | 7.30e-123 | 347 |
| MsG0380014607.01.T01 | AT3G13230 | 75.472 | 212 | 52 | 0 | 3 | 214 | 4 | 215 | 7.30e-123 | 347 |
Find 47 sgRNAs with CRISPR-Local
Find 53 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCTCTTTGTTACCTAATTA+TGG | 0.104484 | 3:+57143770 | MsG0380014607.01.T01:CDS |
| AGATCCCAGAATGTGAATTT+TGG | 0.238915 | 3:-57143721 | None:intergenic |
| CTGCTGGTGATCCCATAATT+AGG | 0.273715 | 3:-57143782 | None:intergenic |
| TGATACCAAAATTCACATTC+TGG | 0.283493 | 3:+57143716 | MsG0380014607.01.T01:CDS |
| AGTTGAGAGCCGTTACTTCT+AGG | 0.295245 | 3:+57143820 | MsG0380014607.01.T01:CDS |
| TGCTATTGCTATTCTGCGTT+TGG | 0.312006 | 3:+57143545 | MsG0380014607.01.T01:CDS |
| TCTCTTTGTTACCTAATTAT+GGG | 0.325990 | 3:+57143771 | MsG0380014607.01.T01:CDS |
| TTTGTTCATGCTTTCATGTT+GGG | 0.347801 | 3:+57143507 | MsG0380014607.01.T01:CDS |
| GGCTCAAACTTTGGCTTTGG+AGG | 0.352039 | 3:-57143264 | None:intergenic |
| AGAGGCTCAAACTTTGGCTT+TGG | 0.356673 | 3:-57143267 | None:intergenic |
| ATACACCTCTCAAGAAAGTT+TGG | 0.369468 | 3:+57143355 | MsG0380014607.01.T01:CDS |
| GATCACTTGTCCCGTGCTAT+TGG | 0.373903 | 3:+57143621 | MsG0380014607.01.T01:CDS |
| CGTGCTATTGGAAGATTATC+TGG | 0.376662 | 3:+57143633 | MsG0380014607.01.T01:CDS |
| TGCATTCTCAATTGTAAACT+TGG | 0.386651 | 3:-57143670 | None:intergenic |
| GGTTTCAGAGGCTCAAACTT+TGG | 0.395402 | 3:-57143273 | None:intergenic |
| TTCGACCCTTCAAATTCATT+CGG | 0.417898 | 3:-57143422 | None:intergenic |
| TGTTGAGTCCTTTGAGATCA+AGG | 0.427401 | 3:+57143578 | MsG0380014607.01.T01:CDS |
| CACAATGGCATCATCTTCAA+TGG | 0.462021 | 3:+57143206 | None:intergenic |
| ATTGGAAGATTATCTGGAAA+AGG | 0.469137 | 3:+57143639 | MsG0380014607.01.T01:CDS |
| TTGCAAACATCAAGATTGCT+AGG | 0.471818 | 3:+57143745 | MsG0380014607.01.T01:CDS |
| AGAAGTAACGGCTCTCAACT+TGG | 0.482912 | 3:-57143817 | None:intergenic |
| TGACATCCGAATGAATTTGA+AGG | 0.495230 | 3:+57143416 | MsG0380014607.01.T01:CDS |
| TTGGAATATACCTTTCCTGC+TGG | 0.496163 | 3:-57143798 | None:intergenic |
| CGAAACTGAACCGCAGCACC+AGG | 0.498813 | 3:-57143306 | None:intergenic |
| AAGGATGTTAAGACTCTTCG+AGG | 0.505057 | 3:+57143597 | MsG0380014607.01.T01:CDS |
| GATACCAAAATTCACATTCT+GGG | 0.511425 | 3:+57143717 | MsG0380014607.01.T01:CDS |
| CTGAAACCTCATGAGATGCC+TGG | 0.516679 | 3:+57143288 | MsG0380014607.01.T01:CDS |
| TGCAAACATCAAGATTGCTA+GGG | 0.524357 | 3:+57143746 | MsG0380014607.01.T01:CDS |
| CTAATTATGGGATCACCAGC+AGG | 0.540701 | 3:+57143783 | MsG0380014607.01.T01:CDS |
| TATGGGATCACCAGCAGGAA+AGG | 0.547596 | 3:+57143788 | MsG0380014607.01.T01:CDS |
| CAGATAATCTTCCAATAGCA+CGG | 0.551311 | 3:-57143632 | None:intergenic |
| GACATCCGAATGAATTTGAA+GGG | 0.559638 | 3:+57143417 | MsG0380014607.01.T01:CDS |
| ACCTCTCAAGAAAGTTTGGA+TGG | 0.563023 | 3:+57143359 | MsG0380014607.01.T01:CDS |
| TCATGAGATGCCTGGTGCTG+CGG | 0.567566 | 3:+57143296 | MsG0380014607.01.T01:CDS |
| TCCATCCAAACTTTCTTGAG+AGG | 0.572045 | 3:-57143360 | None:intergenic |
| GGAAGATTATCTGGAAAAGG+TGG | 0.573573 | 3:+57143642 | MsG0380014607.01.T01:CDS |
| TTCTGCCATCCTAGAAGTAA+CGG | 0.576020 | 3:-57143829 | None:intergenic |
| GAGAGCCGTTACTTCTAGGA+TGG | 0.578516 | 3:+57143824 | MsG0380014607.01.T01:CDS |
| TTACTTCTAGGATGGCAGAA+AGG | 0.584575 | 3:+57143832 | MsG0380014607.01.T01:CDS |
| AGATAATCTTCCAATAGCAC+GGG | 0.591480 | 3:-57143631 | None:intergenic |
| TGCTGCGGTTCAGTTTCGCA+AGG | 0.601400 | 3:+57143311 | MsG0380014607.01.T01:CDS |
| GGCATCTCATGAGGTTTCAG+AGG | 0.608176 | 3:-57143285 | None:intergenic |
| CTTAACATCCTTGATCTCAA+AGG | 0.609805 | 3:-57143586 | None:intergenic |
| GCAGCACCAGGCATCTCATG+AGG | 0.646562 | 3:-57143294 | None:intergenic |
| TTGAGAGGTGTATAACGATG+TGG | 0.685518 | 3:-57143345 | None:intergenic |
| GAAAAGTTGAGTTGAAGACA+AGG | 0.702510 | 3:+57143442 | MsG0380014607.01.T01:CDS |
| AGAGGTGTATAACGATGTGG+TGG | 0.702702 | 3:-57143342 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TCTCTTTGTTACCTAATTAT+GGG | + | Chr3:57143771-57143790 | MsG0380014607.01.T01:CDS | 25.0% |
| ! | TTCTCTTTGTTACCTAATTA+TGG | + | Chr3:57143770-57143789 | MsG0380014607.01.T01:CDS | 25.0% |
| !! | AATTTTCATCTGCTCAAATA+CGG | - | Chr3:57143397-57143416 | None:intergenic | 25.0% |
| ATTGGAAGATTATCTGGAAA+AGG | + | Chr3:57143639-57143658 | MsG0380014607.01.T01:CDS | 30.0% | |
| GATACCAAAATTCACATTCT+GGG | + | Chr3:57143717-57143736 | MsG0380014607.01.T01:CDS | 30.0% | |
| TGATACCAAAATTCACATTC+TGG | + | Chr3:57143716-57143735 | MsG0380014607.01.T01:CDS | 30.0% | |
| TGCATTCTCAATTGTAAACT+TGG | - | Chr3:57143673-57143692 | None:intergenic | 30.0% | |
| TTTGTTCATGCTTTCATGTT+GGG | + | Chr3:57143507-57143526 | MsG0380014607.01.T01:CDS | 30.0% | |
| ! | ATTTTCATCTGCTCAAATAC+GGG | - | Chr3:57143396-57143415 | None:intergenic | 30.0% |
| ! | GCAAAACTTTTTGTTCAGAT+GGG | - | Chr3:57143245-57143264 | None:intergenic | 30.0% |
| ! | TTTTGTTCATGCTTTCATGT+TGG | + | Chr3:57143506-57143525 | MsG0380014607.01.T01:CDS | 30.0% |
| AGATAATCTTCCAATAGCAC+GGG | - | Chr3:57143634-57143653 | None:intergenic | 35.0% | |
| ATACACCTCTCAAGAAAGTT+TGG | + | Chr3:57143355-57143374 | MsG0380014607.01.T01:CDS | 35.0% | |
| CAGATAATCTTCCAATAGCA+CGG | - | Chr3:57143635-57143654 | None:intergenic | 35.0% | |
| CTTAACATCCTTGATCTCAA+AGG | - | Chr3:57143589-57143608 | None:intergenic | 35.0% | |
| GACATCCGAATGAATTTGAA+GGG | + | Chr3:57143417-57143436 | MsG0380014607.01.T01:CDS | 35.0% | |
| TGACATCCGAATGAATTTGA+AGG | + | Chr3:57143416-57143435 | MsG0380014607.01.T01:CDS | 35.0% | |
| TGCAAACATCAAGATTGCTA+GGG | + | Chr3:57143746-57143765 | MsG0380014607.01.T01:CDS | 35.0% | |
| TTCGACCCTTCAAATTCATT+CGG | - | Chr3:57143425-57143444 | None:intergenic | 35.0% | |
| TTGCAAACATCAAGATTGCT+AGG | + | Chr3:57143745-57143764 | MsG0380014607.01.T01:CDS | 35.0% | |
| ! | AGATCCCAGAATGTGAATTT+TGG | - | Chr3:57143724-57143743 | None:intergenic | 35.0% |
| ! | CAAAATCAGCACATTTTTGC+AGG | - | Chr3:57143491-57143510 | None:intergenic | 35.0% |
| ! | GGCAAAACTTTTTGTTCAGA+TGG | - | Chr3:57143246-57143265 | None:intergenic | 35.0% |
| !! | GAAAAGTTGAGTTGAAGACA+AGG | + | Chr3:57143442-57143461 | MsG0380014607.01.T01:CDS | 35.0% |
| AAGGATGTTAAGACTCTTCG+AGG | + | Chr3:57143597-57143616 | MsG0380014607.01.T01:CDS | 40.0% | |
| ACCTCTCAAGAAAGTTTGGA+TGG | + | Chr3:57143359-57143378 | MsG0380014607.01.T01:CDS | 40.0% | |
| GGAAGATTATCTGGAAAAGG+TGG | + | Chr3:57143642-57143661 | MsG0380014607.01.T01:CDS | 40.0% | |
| TCCATCCAAACTTTCTTGAG+AGG | - | Chr3:57143363-57143382 | None:intergenic | 40.0% | |
| TTCTGCCATCCTAGAAGTAA+CGG | - | Chr3:57143832-57143851 | None:intergenic | 40.0% | |
| TTGGAATATACCTTTCCTGC+TGG | - | Chr3:57143801-57143820 | None:intergenic | 40.0% | |
| ! | CGTGCTATTGGAAGATTATC+TGG | + | Chr3:57143633-57143652 | MsG0380014607.01.T01:CDS | 40.0% |
| ! | TGCTATTGCTATTCTGCGTT+TGG | + | Chr3:57143545-57143564 | MsG0380014607.01.T01:CDS | 40.0% |
| ! | TTGAGAGGTGTATAACGATG+TGG | - | Chr3:57143348-57143367 | None:intergenic | 40.0% |
| ! | TTTTGCAGGTTACTGATGTC+AGG | - | Chr3:57143477-57143496 | None:intergenic | 40.0% |
| !! | TGTTGAGTCCTTTGAGATCA+AGG | + | Chr3:57143578-57143597 | MsG0380014607.01.T01:CDS | 40.0% |
| !! | TTACTTCTAGGATGGCAGAA+AGG | + | Chr3:57143832-57143851 | MsG0380014607.01.T01:CDS | 40.0% |
| AGAAGTAACGGCTCTCAACT+TGG | - | Chr3:57143820-57143839 | None:intergenic | 45.0% | |
| AGTTGAGAGCCGTTACTTCT+AGG | + | Chr3:57143820-57143839 | MsG0380014607.01.T01:CDS | 45.0% | |
| CTAATTATGGGATCACCAGC+AGG | + | Chr3:57143783-57143802 | MsG0380014607.01.T01:CDS | 45.0% | |
| CTGCTGGTGATCCCATAATT+AGG | - | Chr3:57143785-57143804 | None:intergenic | 45.0% | |
| GGTTTCAGAGGCTCAAACTT+TGG | - | Chr3:57143276-57143295 | None:intergenic | 45.0% | |
| ! | AGAGGCTCAAACTTTGGCTT+TGG | - | Chr3:57143270-57143289 | None:intergenic | 45.0% |
| ! | AGAGGTGTATAACGATGTGG+TGG | - | Chr3:57143345-57143364 | None:intergenic | 45.0% |
| CTGAAACCTCATGAGATGCC+TGG | + | Chr3:57143288-57143307 | MsG0380014607.01.T01:CDS | 50.0% | |
| GATCACTTGTCCCGTGCTAT+TGG | + | Chr3:57143621-57143640 | MsG0380014607.01.T01:CDS | 50.0% | |
| GGCATCTCATGAGGTTTCAG+AGG | - | Chr3:57143288-57143307 | None:intergenic | 50.0% | |
| TATGGGATCACCAGCAGGAA+AGG | + | Chr3:57143788-57143807 | MsG0380014607.01.T01:CDS | 50.0% | |
| ! | GAGAGCCGTTACTTCTAGGA+TGG | + | Chr3:57143824-57143843 | MsG0380014607.01.T01:CDS | 50.0% |
| !! | GGCTCAAACTTTGGCTTTGG+AGG | - | Chr3:57143267-57143286 | None:intergenic | 50.0% |
| TGCTGCGGTTCAGTTTCGCA+AGG | + | Chr3:57143311-57143330 | MsG0380014607.01.T01:CDS | 55.0% | |
| ! | TCATGAGATGCCTGGTGCTG+CGG | + | Chr3:57143296-57143315 | MsG0380014607.01.T01:CDS | 55.0% |
| CGAAACTGAACCGCAGCACC+AGG | - | Chr3:57143309-57143328 | None:intergenic | 60.0% | |
| GCAGCACCAGGCATCTCATG+AGG | - | Chr3:57143297-57143316 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 57143210 | 57143860 | 57143210 | ID=MsG0380014607.01;Name=MsG0380014607.01 |
| Chr3 | mRNA | 57143210 | 57143860 | 57143210 | ID=MsG0380014607.01.T01;Parent=MsG0380014607.01;Name=MsG0380014607.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|216 |
| Chr3 | exon | 57143210 | 57143860 | 57143210 | ID=MsG0380014607.01.T01:exon:16180;Parent=MsG0380014607.01.T01 |
| Chr3 | CDS | 57143210 | 57143860 | 57143210 | ID=MsG0380014607.01.T01:cds;Parent=MsG0380014607.01.T01 |
| Gene Sequence |
| Protein sequence |
