AlfalfaGEDB | MsG0480023363.01

M. sativa cultivar ZhongmuNo.1 / MsG0480023363.01

KEGG: K14431
GO: GO:0003700 GO:0006351 GO:0006355 GO:0043565
PFAM: PF00170

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480023363.01.T01	XP_013446843.1	95.413	109	5	0	116	224	159	267	4.05E-64	211
MsG0480023363.01.T01	XP_013446843.1	95.833	48	2	0	71	118	2	49	3.18E-18	92

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480023363.01.T01	Q39162	67.89	109	35	0	116	224	238	346	6.83E-42	148

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480023363.01.T01	A0A072U4Y3	95.413	109	5	0	116	224	159	267	1.94e-64	211

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048158.01	MsG0480023363.01	0.807131	5.872816e-50	3.244884e-47
MsG0180005959.01	MsG0480023363.01	0.824561	7.549231e-54	6.711018e-51
MsG0480019106.01	MsG0480023363.01	0.814297	1.655148e-51	1.106403e-48
MsG0480021996.01	MsG0480023363.01	0.829568	4.787568e-55	4.918287e-52
MsG0480023363.01	MsG0580027993.01	0.837546	4.877655e-57	6.357086e-54
MsG0480023363.01	MsG0680035842.01	0.801794	7.610822e-49	3.667499e-46
MsG0480023363.01	MsG0880045861.01	0.808414	3.134057e-50	1.790854e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480023363.01.T01	MTR_8g091650	95.413	109	5	0	116	224	159	267	4.91e-68	211
MsG0480023363.01.T01	MTR_8g091650	95.833	48	2	0	71	118	2	49	3.85e-22	92.0
MsG0480023363.01.T01	MTR_8g091650	95.413	109	5	0	116	224	232	340	1.96e-67	211
MsG0480023363.01.T01	MTR_8g091650	81.967	122	18	2	1	118	1	122	1.76e-57	186
MsG0480023363.01.T01	MTR_8g091650	95.413	109	5	0	116	224	268	376	6.99e-67	211
MsG0480023363.01.T01	MTR_8g091650	63.291	158	18	3	1	118	1	158	4.94e-50	167
MsG0480023363.01.T01	MTR_3g087940	71.560	109	31	0	116	224	237	345	2.01e-47	160
MsG0480023363.01.T01	MTR_3g087940	51.639	122	50	3	3	116	4	124	7.02e-28	108
MsG0480023363.01.T01	MTR_7g089800	50.427	117	51	2	116	225	292	408	1.48e-25	103
MsG0480023363.01.T01	MTR_7g089800	73.913	46	12	0	71	116	138	183	3.43e-13	68.2

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480023363.01.T01	AT5G10030	67.890	109	35	0	116	224	238	346	6.96e-43	148
MsG0480023363.01.T01	AT5G10030	46.774	124	52	5	4	116	5	125	1.10e-20	89.4
MsG0480023363.01.T01	AT5G10030	67.890	109	35	0	116	224	238	346	6.96e-43	148
MsG0480023363.01.T01	AT5G10030	46.774	124	52	5	4	116	5	125	1.10e-20	89.4
MsG0480023363.01.T01	AT5G10030	67.890	109	35	0	116	224	238	346	6.96e-43	148
MsG0480023363.01.T01	AT5G10030	46.774	124	52	5	4	116	5	125	1.10e-20	89.4
MsG0480023363.01.T01	AT5G10030	67.890	109	35	0	116	224	238	346	6.96e-43	148
MsG0480023363.01.T01	AT5G10030	46.774	124	52	5	4	116	5	125	1.10e-20	89.4
MsG0480023363.01.T01	AT5G10030	67.890	109	35	0	116	224	238	346	6.96e-43	148
MsG0480023363.01.T01	AT5G10030	46.774	124	52	5	4	116	5	125	1.10e-20	89.4

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Find 43 sgRNAs with CRISPR-Local

Find 193 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGGGCATCTTCTGCTTGT+CGG	0.484492	4:-84209310	None:intergenic
AACCATTGACAGAGGAACAA+CGG	0.614266	4:+84209258	MsG0480023363.01.T01:CDS
AACCGTTGTTCCTCTGTCAA+TGG	0.441867	4:-84209260	None:intergenic
AATAGAGAGGCTGGAAGCTC+TGG	0.410741	4:+84209427	MsG0480023363.01.T01:CDS
ACAGGCTGATCATCTTCGAC+AGG	0.558370	4:+84209709	MsG0480023363.01.T01:intron
ACGTCTCGCACAAAATCGAG+AGG	0.680873	4:+84207653	MsG0480023363.01.T01:CDS
AGAGCTAGATCATACAAAAC+AGG	0.481991	4:+84207834	MsG0480023363.01.T01:CDS
AGATGCCCTTTCACAAGGTA+TGG	0.480625	4:+84209322	MsG0480023363.01.T01:CDS
AGATGGATATTGCAATAGAG+AGG	0.647680	4:+84209414	MsG0480023363.01.T01:CDS
AGCTAACACAGTTACTAATA+AGG	0.363384	4:+84207082	MsG0480023363.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AACCTACAAAATCAACTACA+AGG	+	Chr4:84208153-84208172	MsG0480023363.01.T01:intron	30.0%
ACCTACAAAATCAACTACAA+GGG	+	Chr4:84208154-84208173	MsG0480023363.01.T01:intron	30.0%
AGAATAGAGTTCAATACAAG+AGG	-	Chr4:84208811-84208830	None:intergenic	30.0%
AGACAAATGTTGGAATTGAA+AGG	+	Chr4:84208920-84208939	MsG0480023363.01.T01:intron	30.0%
AGCTAACACAGTTACTAATA+AGG	+	Chr4:84207082-84207101	MsG0480023363.01.T01:CDS	30.0%
ATCGATATACTTGATCAACA+TGG	+	Chr4:84209152-84209171	MsG0480023363.01.T01:intron	30.0%
ATTCCAACATTTGTCTGTTT+AGG	-	Chr4:84208916-84208935	None:intergenic	30.0%
ATTGCTCAATCAGTATTTCA+TGG	+	Chr4:84207432-84207451	MsG0480023363.01.T01:intron	30.0%
CCATACACCATTTACTATAT+GGG	+	Chr4:84206835-84206854	MsG0480023363.01.T01:CDS	30.0%
CCATATAGTAAATGGTGTAT+GGG	-	Chr4:84206837-84206856	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr4	gene	84206782	84209817	84206782	ID=MsG0480023363.01;Name=MsG0480023363.01
Chr4	mRNA	84206782	84209817	84206782	ID=MsG0480023363.01.T01;Parent=MsG0480023363.01;Name=MsG0480023363.01.T01;_AED=0.40;_eAED=0.40;_QI=0\|0\|0\|1\|1\|1\|6\|0\|226
Chr4	exon	84206782	84206921	84206782	ID=MsG0480023363.01.T01:exon:1258;Parent=MsG0480023363.01.T01
Chr4	exon	84207031	84207103	84207031	ID=MsG0480023363.01.T01:exon:1259;Parent=MsG0480023363.01.T01
Chr4	exon	84207645	84207704	84207645	ID=MsG0480023363.01.T01:exon:1260;Parent=MsG0480023363.01.T01
Chr4	exon	84207781	84207855	84207781	ID=MsG0480023363.01.T01:exon:1261;Parent=MsG0480023363.01.T01
Chr4	exon	84209239	84209466	84209239	ID=MsG0480023363.01.T01:exon:1262;Parent=MsG0480023363.01.T01
Chr4	exon	84209713	84209817	84209713	ID=MsG0480023363.01.T01:exon:1263;Parent=MsG0480023363.01.T01
Chr4	CDS	84206782	84206921	84206782	ID=MsG0480023363.01.T01:cds;Parent=MsG0480023363.01.T01
Chr4	CDS	84207031	84207103	84207031	ID=MsG0480023363.01.T01:cds;Parent=MsG0480023363.01.T01
Chr4	CDS	84207645	84207704	84207645	ID=MsG0480023363.01.T01:cds;Parent=MsG0480023363.01.T01
Chr4	CDS	84207781	84207855	84207781	ID=MsG0480023363.01.T01:cds;Parent=MsG0480023363.01.T01
Chr4	CDS	84209239	84209466	84209239	ID=MsG0480023363.01.T01:cds;Parent=MsG0480023363.01.T01
Chr4	CDS	84209713	84209817	84209713	ID=MsG0480023363.01.T01:cds;Parent=MsG0480023363.01.T01

Gene Sequence

>MsG0480023363.01.T01

ATGGATCCTTCATCATCTTCAGTTTCCTCAAGAAATATTGGTGTATATGATTCCCATACACCATTTACTATATGGGAAGAACATTTCAAGAGTAATGGTGATACAAGTGTATATGAACCTGAAATGAAGTTAAATATCAGAGGATGCTTCTTTTGGAATATTGGAACATCTATCAAGTATGACCACCAAGAAGCTAACACAGTTACTAATAAGATGCAAAGACGTCTCGCACAAAATCGAGAGGCTGCTCGTAAGAGTCGTTTGAAGAAAAAGGCTCATATACAACAGTTAGAATCGTGTCGTTTAAAGCTGTTACAGGTAGAGCAAGAGCTAGATCATACAAAACAGGTTCTGGTCCCTTTAATTGAACCATTGACAGAGGAACAACGGTTTGATGCCTATAATCTTGAGAAGTCATGCCGACAAGCAGAAGATGCCCTTTCACAAGGTATGGAAAAACTACAGGGAATGCTTGTTGACACAGTTGCAGCAGGACAACTCGTCGAAGGAACTTACATTCCACAGATGGATATTGCAATAGAGAGGCTGGAAGCTCTGGCTAGCTTTGTGAACCAGGCTGATCATCTTCGACAGGAAACGTTGCAACAGATGTCTCGAATACTTACCGTACGTCAAACTGCTCGATGGCTGCTTGCTTGGGTGAATACTTTCAACGCCTAA

Protein sequence

>MsG0480023363.01.T01

MDPSSSSVSSRNIGVYDSHTPFTIWEEHFKSNGDTSVYEPEMKLNIRGCFFWNIGTSIKYDHQEANTVTNKMQRRLAQNREAARKSRLKKKAHIQQLESCRLKLLQVEQELDHTKQVLVPLIEPLTEEQRFDAYNLEKSCRQAEDALSQGMEKLQGMLVDTVAAGQLVEGTYIPQMDIAIERLEALASFVNQADHLRQETLQQMSRILTVRQTARWLLAWVNTFNA*