AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480023372.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480023372.01.T01 MTR_8g091720 93.717 191 5 1 1 184 1 191 1.02e-130 365
MsG0480023372.01.T01 MTR_5g095740 79.487 117 22 1 13 129 10 124 8.38e-63 192
MsG0480023372.01.T01 MTR_2g026710 80.702 114 20 1 24 137 13 124 2.67e-62 191
MsG0480023372.01.T01 MTR_3g058980 87.879 99 12 0 25 123 24 122 4.19e-60 186
MsG0480023372.01.T01 MTR_7g061270 75.439 114 28 0 13 126 11 124 1.36e-59 184
MsG0480023372.01.T01 MTR_7g100650 75.439 114 28 0 13 126 11 124 1.39e-59 184
MsG0480023372.01.T01 MTR_8g093920 76.636 107 25 0 27 133 39 145 1.49e-56 178
MsG0480023372.01.T01 MTR_1g072790 76.699 103 24 0 23 125 24 126 2.35e-56 176
MsG0480023372.01.T01 MTR_4g133938 74.312 109 28 0 17 125 8 116 2.25e-53 167
MsG0480023372.01.T01 MTR_1g088860 69.307 101 31 0 22 122 30 130 1.89e-48 157
MsG0480023372.01.T01 MTR_0392s0020 64.151 106 38 0 21 126 11 116 2.78e-48 154
MsG0480023372.01.T01 MTR_4g119500 56.557 122 53 0 12 133 6 127 2.55e-47 151
MsG0480023372.01.T01 MTR_4g133952 64.948 97 34 0 27 123 57 153 5.01e-46 152
MsG0480023372.01.T01 MTR_1g039040 62.745 102 38 0 27 128 5 106 1.43e-44 146
MsG0480023372.01.T01 MTR_1g083070 61.957 92 35 0 28 119 4 95 7.95e-39 130
MsG0480023372.01.T01 MTR_1g029070 49.580 119 54 1 14 126 32 150 2.53e-32 115
MsG0480023372.01.T01 MTR_5g095900 44.330 97 51 1 24 117 9 105 1.54e-21 87.4
MsG0480023372.01.T01 MTR_1g028480 53.125 64 30 0 58 121 19 82 2.19e-18 77.0
MsG0480023372.01.T01 MTR_1g029100 40.789 76 45 0 33 108 7 82 1.95e-17 73.9
MsG0480023372.01.T01 MTR_2g056000 32.143 84 57 0 22 105 35 118 1.23e-13 66.6
MsG0480023372.01.T01 MTR_4g112380 33.766 77 51 0 29 105 12 88 2.68e-13 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480023372.01.T01 AT4G14540 81.818 110 20 0 24 133 17 126 3.78e-64 195
MsG0480023372.01.T01 AT5G47640 85.859 99 14 0 25 123 24 122 6.71e-60 186
MsG0480023372.01.T01 AT2G13570 74.576 118 29 1 8 125 17 133 1.99e-59 185
MsG0480023372.01.T01 AT3G53340 68.800 125 28 2 14 133 21 139 1.94e-56 176
MsG0480023372.01.T01 AT3G53340 68.800 125 28 2 14 133 21 139 1.94e-56 176
MsG0480023372.01.T01 AT2G37060 71.552 116 28 1 27 137 29 144 4.70e-56 175
MsG0480023372.01.T01 AT2G37060 71.552 116 28 1 27 137 29 144 4.70e-56 175
MsG0480023372.01.T01 AT2G37060 71.552 116 28 1 27 137 29 144 4.70e-56 175
MsG0480023372.01.T01 AT2G38880 69.643 112 34 0 14 125 7 118 8.05e-55 171
MsG0480023372.01.T01 AT3G53340 75.238 105 20 1 14 118 21 119 9.34e-55 170
MsG0480023372.01.T01 AT2G38880 69.643 112 34 0 14 125 7 118 1.25e-54 171
MsG0480023372.01.T01 AT2G38880 69.643 112 34 0 14 125 7 118 1.25e-54 171
MsG0480023372.01.T01 AT2G38880 69.643 112 34 0 14 125 7 118 1.25e-54 171
MsG0480023372.01.T01 AT3G53340 75.472 106 20 1 14 119 21 120 2.76e-54 170
MsG0480023372.01.T01 AT3G53340 75.472 106 20 1 14 119 21 120 2.76e-54 170
MsG0480023372.01.T01 AT2G38880 71.429 105 30 0 14 118 7 111 1.17e-52 164
MsG0480023372.01.T01 AT2G38880 67.544 114 37 0 14 127 7 120 1.44e-52 165
MsG0480023372.01.T01 AT2G38880 71.429 105 30 0 14 118 7 111 3.44e-52 165
MsG0480023372.01.T01 AT2G38880 71.429 105 30 0 14 118 7 111 3.44e-52 165
MsG0480023372.01.T01 AT2G38880 77.660 94 21 0 25 118 18 111 5.53e-52 164
MsG0480023372.01.T01 AT2G38880 77.660 94 21 0 25 118 18 111 5.53e-52 164
MsG0480023372.01.T01 AT2G38880 77.660 94 21 0 25 118 18 111 5.53e-52 164
MsG0480023372.01.T01 AT2G47810 71.845 103 29 0 22 124 45 147 3.85e-51 162
MsG0480023372.01.T01 AT5G47670 60.714 112 44 0 16 127 17 128 6.27e-48 155
MsG0480023372.01.T01 AT5G47670 60.714 112 44 0 16 127 46 157 1.55e-47 155
MsG0480023372.01.T01 AT5G47670 60.714 112 44 0 16 127 46 157 1.55e-47 155
MsG0480023372.01.T01 AT1G21970 62.000 100 38 0 26 125 57 156 2.57e-45 150
MsG0480023372.01.T01 AT1G09030 62.500 96 36 0 28 123 3 98 1.26e-42 140
MsG0480023372.01.T01 AT5G08190 35.897 78 50 0 28 105 11 88 1.74e-14 68.2
MsG0480023372.01.T01 AT5G08190 35.897 78 50 0 28 105 11 88 1.87e-14 68.2
MsG0480023372.01.T01 AT5G23090 33.766 77 51 0 29 105 12 88 5.10e-14 67.0
MsG0480023372.01.T01 AT5G23090 33.766 77 51 0 29 105 12 88 5.10e-14 67.0
MsG0480023372.01.T01 AT5G23090 33.766 77 51 0 29 105 12 88 5.10e-14 67.0
MsG0480023372.01.T01 AT5G23090 33.766 77 51 0 29 105 12 88 5.10e-14 67.0

Find 42 sgRNAs with CRISPR-Local

Find 45 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTAATGGTGATGATCTTCTT+TGG 0.277181 4:+84297019 MsG0480023372.01.T01:CDS
TTAATCTCTTGGAACCTCTT+TGG 0.292077 4:-84297216 None:intergenic
AAGTTTATCTTAATAATTAT+AGG 0.297968 4:+84297085 MsG0480023372.01.T01:CDS
GAGTTCATAAGTTTCATTAC+TGG 0.304041 4:+84296955 MsG0480023372.01.T01:CDS
TGCCACCAAACCTACGTTAC+AGG 0.318400 4:+84297316 MsG0480023372.01.T01:CDS
GAAAAGAGGAAAACAATTAA+TGG 0.329176 4:+84297003 MsG0480023372.01.T01:CDS
AGTTTATCTTAATAATTATA+GGG 0.334719 4:+84297086 MsG0480023372.01.T01:CDS
ATTGTGGATTATAGAGTAAT+TGG 0.335947 4:+84297246 MsG0480023372.01.T01:CDS
AAGAGGTTCCAAGAGATTAA+TGG 0.350652 4:+84297219 MsG0480023372.01.T01:CDS
ATTATTAATTTCAACATGTC+CGG 0.359734 4:+84296745 None:intergenic
TAATGGTGATGATCTTCTTT+GGG 0.364795 4:+84297020 MsG0480023372.01.T01:CDS
GACTTCCTACAGGACTTGTT+TGG 0.366205 4:-84296780 None:intergenic
TGTTTGGTTTCTCTTATTCC+CGG 0.387356 4:-84296764 None:intergenic
CAATCCCTCCATTAATCTCT+TGG 0.406112 4:-84297227 None:intergenic
GATGAAACAGAACATGCAAT+TGG 0.429214 4:+84297276 MsG0480023372.01.T01:CDS
CCTGATGTTGGACTTCCTAC+AGG 0.434628 4:-84296790 None:intergenic
ATACGGCTCACGTTGGCAAT+TGG 0.452960 4:-84296859 None:intergenic
TCTGAGATGTTTCCTGATGT+TGG 0.453105 4:-84296802 None:intergenic
CTTTGGGCTATGACAACACT+TGG 0.464387 4:+84297036 MsG0480023372.01.T01:CDS
AATAATTATAGGGAAATTGA+AGG 0.468083 4:+84297096 MsG0480023372.01.T01:CDS
TCAACCCTGTAACGTAGGTT+TGG 0.472415 4:-84297321 None:intergenic
TCAATTGATGTTAATGATGT+TGG 0.481755 4:+84297177 MsG0480023372.01.T01:CDS
CCTGTAGGAAGTCCAACATC+AGG 0.498605 4:+84296790 MsG0480023372.01.T01:CDS
ACCTACGTTACAGGGTTGAA+TGG 0.502904 4:+84297325 MsG0480023372.01.T01:CDS
TTATTAATTTCAACATGTCC+GGG 0.509564 4:+84296746 None:intergenic
AGAGATTAATGGAGGGATTG+TGG 0.513734 4:+84297230 MsG0480023372.01.T01:CDS
ACAGAACATGCAATTGGAAG+TGG 0.518322 4:+84297282 MsG0480023372.01.T01:CDS
CTCAAAAGAAGCAAAGGAAA+CGG 0.525390 4:+84296915 MsG0480023372.01.T01:CDS
ATTGATGTTAATGATGTTGG+TGG 0.525414 4:+84297180 MsG0480023372.01.T01:CDS
ACCCTGTAACGTAGGTTTGG+TGG 0.528618 4:-84297318 None:intergenic
GATGTTAATGATGTTGGTGG+TGG 0.530464 4:+84297183 MsG0480023372.01.T01:CDS
CATCATCAAAAGAACAAGAC+AGG 0.530857 4:+84296830 MsG0480023372.01.T01:CDS
ATAAGTGCCAGAGAGAAAAG+AGG 0.537335 4:+84296989 MsG0480023372.01.T01:CDS
TTATTAAGATAAACTTTCAA+AGG 0.539135 4:-84297078 None:intergenic
GCCACCAAACCTACGTTACA+GGG 0.557356 4:+84297317 MsG0480023372.01.T01:CDS
TAAAATCTCAAAAGAAGCAA+AGG 0.579705 4:+84296909 MsG0480023372.01.T01:CDS
AGAAACCAAACAAGTCCTGT+AGG 0.592028 4:+84296775 MsG0480023372.01.T01:CDS
GGCTCACGTTGGCAATTGGT+AGG 0.609486 4:-84296855 None:intergenic
ACCATTCAACCCTGTAACGT+AGG 0.617328 4:-84297326 None:intergenic
AGGTTCCAAGAGATTAATGG+AGG 0.632716 4:+84297222 MsG0480023372.01.T01:CDS
GGTTCCAAGAGATTAATGGA+GGG 0.665223 4:+84297223 MsG0480023372.01.T01:CDS
TTTCATTATACGGCTCACGT+TGG 0.672250 4:-84296866 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGTTTATCTTAATAATTAT+AGG + Chr4:84297085-84297104 MsG0480023372.01.T01:CDS 10.0%
!! AGTTTATCTTAATAATTATA+GGG + Chr4:84297086-84297105 MsG0480023372.01.T01:CDS 10.0%
!! TTATTAAGATAAACTTTCAA+AGG - Chr4:84297081-84297100 None:intergenic 15.0%
!! AATAATTATAGGGAAATTGA+AGG + Chr4:84297096-84297115 MsG0480023372.01.T01:CDS 20.0%
! GAAAAGAGGAAAACAATTAA+TGG + Chr4:84297003-84297022 MsG0480023372.01.T01:CDS 25.0%
! TAAAATCTCAAAAGAAGCAA+AGG + Chr4:84296909-84296928 MsG0480023372.01.T01:CDS 25.0%
! TCAATTGATGTTAATGATGT+TGG + Chr4:84297177-84297196 MsG0480023372.01.T01:CDS 25.0%
!! ATTGTGGATTATAGAGTAAT+TGG + Chr4:84297246-84297265 MsG0480023372.01.T01:CDS 25.0%
ATTGATGTTAATGATGTTGG+TGG + Chr4:84297180-84297199 MsG0480023372.01.T01:CDS 30.0%
GAGTTCATAAGTTTCATTAC+TGG + Chr4:84296955-84296974 MsG0480023372.01.T01:CDS 30.0%
! TAATGGTGATGATCTTCTTT+GGG + Chr4:84297020-84297039 MsG0480023372.01.T01:CDS 30.0%
! TTAATGGTGATGATCTTCTT+TGG + Chr4:84297019-84297038 MsG0480023372.01.T01:CDS 30.0%
!! GAAGTGCTCTTTTCATTATA+CGG - Chr4:84296879-84296898 None:intergenic 30.0%
!! TTTGAGATTTTAGCATTTGC+TGG - Chr4:84296901-84296920 None:intergenic 30.0%
!!! CTTGGTTTTGAAAACTATGT+TGG + Chr4:84297054-84297073 MsG0480023372.01.T01:CDS 30.0%
AAGAGGTTCCAAGAGATTAA+TGG + Chr4:84297219-84297238 MsG0480023372.01.T01:CDS 35.0%
CATCATCAAAAGAACAAGAC+AGG + Chr4:84296830-84296849 MsG0480023372.01.T01:CDS 35.0%
CTCAAAAGAAGCAAAGGAAA+CGG + Chr4:84296915-84296934 MsG0480023372.01.T01:CDS 35.0%
GATGAAACAGAACATGCAAT+TGG + Chr4:84297276-84297295 MsG0480023372.01.T01:CDS 35.0%
TTAATCTCTTGGAACCTCTT+TGG - Chr4:84297219-84297238 None:intergenic 35.0%
! TGTTTGGTTTCTCTTATTCC+CGG - Chr4:84296767-84296786 None:intergenic 35.0%
! TTGTTTTCCTCTTTTCTCTC+TGG - Chr4:84296999-84297018 None:intergenic 35.0%
ACAGAACATGCAATTGGAAG+TGG + Chr4:84297282-84297301 MsG0480023372.01.T01:CDS 40.0%
AGAAACCAAACAAGTCCTGT+AGG + Chr4:84296775-84296794 MsG0480023372.01.T01:CDS 40.0%
AGAGATTAATGGAGGGATTG+TGG + Chr4:84297230-84297249 MsG0480023372.01.T01:CDS 40.0%
AGGTTCCAAGAGATTAATGG+AGG + Chr4:84297222-84297241 MsG0480023372.01.T01:CDS 40.0%
ATAAGTGCCAGAGAGAAAAG+AGG + Chr4:84296989-84297008 MsG0480023372.01.T01:CDS 40.0%
CAATCCCTCCATTAATCTCT+TGG - Chr4:84297230-84297249 None:intergenic 40.0%
GGTTCCAAGAGATTAATGGA+GGG + Chr4:84297223-84297242 MsG0480023372.01.T01:CDS 40.0%
TTTCATTATACGGCTCACGT+TGG - Chr4:84296869-84296888 None:intergenic 40.0%
! GATGTTAATGATGTTGGTGG+TGG + Chr4:84297183-84297202 MsG0480023372.01.T01:CDS 40.0%
! GTGGATTTTATGCTCCAAAG+AGG + Chr4:84297202-84297221 MsG0480023372.01.T01:CDS 40.0%
! TCTGAGATGTTTCCTGATGT+TGG - Chr4:84296805-84296824 None:intergenic 40.0%
ACCATTCAACCCTGTAACGT+AGG - Chr4:84297329-84297348 None:intergenic 45.0%
ACCTACGTTACAGGGTTGAA+TGG + Chr4:84297325-84297344 MsG0480023372.01.T01:CDS 45.0%
CTTTGGGCTATGACAACACT+TGG + Chr4:84297036-84297055 MsG0480023372.01.T01:CDS 45.0%
TCAACCCTGTAACGTAGGTT+TGG - Chr4:84297324-84297343 None:intergenic 45.0%
! GACTTCCTACAGGACTTGTT+TGG - Chr4:84296783-84296802 None:intergenic 45.0%
ACCCTGTAACGTAGGTTTGG+TGG - Chr4:84297321-84297340 None:intergenic 50.0%
ATACGGCTCACGTTGGCAAT+TGG - Chr4:84296862-84296881 None:intergenic 50.0%
CCTGATGTTGGACTTCCTAC+AGG - Chr4:84296793-84296812 None:intergenic 50.0%
CCTGTAGGAAGTCCAACATC+AGG + Chr4:84296790-84296809 MsG0480023372.01.T01:CDS 50.0%
GCCACCAAACCTACGTTACA+GGG + Chr4:84297317-84297336 MsG0480023372.01.T01:CDS 50.0%
TGCCACCAAACCTACGTTAC+AGG + Chr4:84297316-84297335 MsG0480023372.01.T01:CDS 50.0%
GGCTCACGTTGGCAATTGGT+AGG - Chr4:84296858-84296877 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
Chr4 gene 84296760 84297350 84296760 ID=MsG0480023372.01;Name=MsG0480023372.01
Chr4 mRNA 84296760 84297350 84296760 ID=MsG0480023372.01.T01;Parent=MsG0480023372.01;Name=MsG0480023372.01.T01;_AED=0.38;_eAED=0.38;_QI=0|-1|0|1|-1|1|1|0|196
Chr4 exon 84296760 84297350 84296760 ID=MsG0480023372.01.T01:exon:4737;Parent=MsG0480023372.01.T01
Chr4 CDS 84296760 84297350 84296760 ID=MsG0480023372.01.T01:cds;Parent=MsG0480023372.01.T01
Gene Sequence

>MsG0480023372.01.T01

ATGTCCGGGAATAAGAGAAACCAAACAAGTCCTGTAGGAAGTCCAACATCAGGAAACATCTCAGACAGTTCATCATCAAAAGAACAAGACAGGTTCCTACCAATTGCCAACGTGAGCCGTATAATGAAAAGAGCACTTCCAGCAAATGCTAAAATCTCAAAAGAAGCAAAGGAAACGGTTCAAGAATGTGTCTCTGAGTTCATAAGTTTCATTACTGGTGAAGCTTCTGATAAGTGCCAGAGAGAAAAGAGGAAAACAATTAATGGTGATGATCTTCTTTGGGCTATGACAACACTTGGTTTTGAAAACTATGTTGGTCCTTTGAAAGTTTATCTTAATAATTATAGGGAAATTGAAGGAGAAAAGAGTAATTCTAGTGCTACTAAACAAGATGATCAATATGATCATAATTCTTCTTCAATTGATGTTAATGATGTTGGTGGTGGATTTTATGCTCCAAAGAGGTTCCAAGAGATTAATGGAGGGATTGTGGATTATAGAGTAATTGGTAATAGTGATGAAACAGAACATGCAATTGGAAGTGGTAATAGAATCATGCCACCAAACCTACGTTACAGGGTTGAATGGTAG

Protein sequence

>MsG0480023372.01.T01

MSGNKRNQTSPVGSPTSGNISDSSSSKEQDRFLPIANVSRIMKRALPANAKISKEAKETVQECVSEFISFITGEASDKCQREKRKTINGDDLLWAMTTLGFENYVGPLKVYLNNYREIEGEKSNSSATKQDDQYDHNSSSIDVNDVGGGFYAPKRFQEINGGIVDYRVIGNSDETEHAIGSGNRIMPPNLRYRVEW*