Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480023624.01.T01 | XP_013447024.1 | 83.495 | 206 | 30 | 2 | 1 | 203 | 1 | 205 | 1.21E-120 | 350 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480023624.01.T01 | Q9SH27 | 44.175 | 206 | 95 | 7 | 1 | 202 | 1 | 190 | 2.77E-43 | 147 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|---|---|
| MsG0480023624.01 | MsG0780039359.01 | PPI |
| MsG0480023624.01 | MsG0780039372.01 | PPI |
| MsG0480023624.01 | MsG0780039372.01 | PPI |
| MsG0480023624.01 | MsG0780039370.01 | PPI |
| MsG0480023624.01 | MsG0480023625.01 | PPI |
| MsG0480023624.01 | MsG0780039368.01 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480023624.01.T01 | MTR_8g097400 | 83.495 | 206 | 30 | 2 | 1 | 203 | 1 | 205 | 1.46e-124 | 350 |
| MsG0480023624.01.T01 | MTR_8g097390 | 83.495 | 206 | 25 | 3 | 1 | 203 | 1 | 200 | 5.94e-124 | 348 |
| MsG0480023624.01.T01 | MTR_8g097410 | 80.097 | 206 | 37 | 2 | 1 | 203 | 1 | 205 | 6.01e-124 | 348 |
| MsG0480023624.01.T01 | MTR_8g097380 | 81.068 | 206 | 35 | 2 | 1 | 203 | 1 | 205 | 5.88e-123 | 346 |
| MsG0480023624.01.T01 | MTR_0415s0060 | 83.010 | 206 | 26 | 3 | 1 | 203 | 1 | 200 | 7.16e-121 | 340 |
| MsG0480023624.01.T01 | MTR_8g097370 | 80.749 | 187 | 28 | 2 | 1 | 187 | 1 | 179 | 5.38e-109 | 311 |
| MsG0480023624.01.T01 | MTR_8g023060 | 39.512 | 205 | 108 | 5 | 1 | 199 | 1 | 195 | 2.74e-37 | 128 |
| MsG0480023624.01.T01 | MTR_0559s0010 | 63.953 | 86 | 27 | 2 | 1 | 83 | 1 | 85 | 1.18e-31 | 110 |
| MsG0480023624.01.T01 | MTR_0559s0020 | 84.615 | 39 | 6 | 0 | 162 | 200 | 10 | 48 | 2.30e-16 | 70.9 |
| MsG0480023624.01.T01 | MTR_2g103850 | 31.285 | 179 | 109 | 6 | 30 | 201 | 33 | 204 | 2.48e-14 | 69.3 |
| MsG0480023624.01.T01 | MTR_8g099000 | 30.601 | 183 | 109 | 7 | 30 | 195 | 46 | 227 | 7.06e-12 | 62.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480023624.01.T01 | AT1G63410 | 43.961 | 207 | 96 | 7 | 1 | 203 | 1 | 191 | 1.20e-44 | 147 |
| MsG0480023624.01.T01 | AT1G63410 | 44.175 | 206 | 95 | 7 | 1 | 202 | 1 | 190 | 2.82e-44 | 147 |
| MsG0480023624.01.T01 | AT3G14260 | 37.198 | 207 | 119 | 5 | 1 | 199 | 23 | 226 | 2.47e-38 | 132 |
| MsG0480023624.01.T01 | AT1G53890 | 36.792 | 212 | 108 | 8 | 1 | 198 | 13 | 212 | 6.71e-34 | 120 |
| MsG0480023624.01.T01 | AT1G53870 | 36.792 | 212 | 108 | 8 | 1 | 198 | 13 | 212 | 6.71e-34 | 120 |
| MsG0480023624.01.T01 | AT1G53870 | 36.792 | 212 | 108 | 8 | 1 | 198 | 13 | 212 | 6.71e-34 | 120 |
| MsG0480023624.01.T01 | AT1G53890 | 36.792 | 212 | 108 | 8 | 1 | 198 | 13 | 212 | 6.71e-34 | 120 |
| MsG0480023624.01.T01 | AT1G53875 | 35.849 | 212 | 111 | 7 | 1 | 199 | 25 | 224 | 2.94e-32 | 116 |
| MsG0480023624.01.T01 | AT1G53890 | 36.458 | 192 | 96 | 8 | 1 | 178 | 13 | 192 | 2.79e-28 | 105 |
| MsG0480023624.01.T01 | AT1G53870 | 36.458 | 192 | 96 | 8 | 1 | 178 | 13 | 192 | 2.79e-28 | 105 |
| MsG0480023624.01.T01 | AT1G53870 | 36.458 | 192 | 96 | 8 | 1 | 178 | 13 | 192 | 2.79e-28 | 105 |
| MsG0480023624.01.T01 | AT1G53890 | 36.458 | 192 | 96 | 8 | 1 | 178 | 13 | 192 | 2.79e-28 | 105 |
| MsG0480023624.01.T01 | AT1G63410 | 39.634 | 164 | 79 | 7 | 1 | 160 | 1 | 148 | 1.32e-25 | 97.4 |
| MsG0480023624.01.T01 | AT1G53900 | 37.870 | 169 | 79 | 6 | 22 | 178 | 40 | 194 | 1.93e-24 | 100 |
| MsG0480023624.01.T01 | AT1G53900 | 37.870 | 169 | 79 | 6 | 22 | 178 | 25 | 179 | 1.95e-24 | 100 |
| MsG0480023624.01.T01 | AT1G53900 | 37.870 | 169 | 79 | 6 | 22 | 178 | 49 | 203 | 2.24e-24 | 100 |
| MsG0480023624.01.T01 | AT1G53890 | 33.508 | 191 | 91 | 7 | 1 | 178 | 13 | 180 | 4.10e-23 | 93.2 |
| MsG0480023624.01.T01 | AT2G38640 | 26.733 | 202 | 127 | 7 | 1 | 195 | 1 | 188 | 1.40e-11 | 61.2 |
Find 38 sgRNAs with CRISPR-Local
Find 49 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCTTTCTATACACAAATTT+TGG | 0.062529 | 4:+87787295 | None:intergenic |
| GAAACCATGGTTGTTCCTTT+TGG | 0.275199 | 4:+87787379 | None:intergenic |
| CGATGTTCTAACTTTGGATT+TGG | 0.278695 | 4:-87787169 | MsG0480023624.01.T01:CDS |
| CCGTTACCAAAGCCATTATA+AGG | 0.296552 | 4:+87787127 | None:intergenic |
| CACAGATCATTCCCTTATAA+TGG | 0.302815 | 4:-87787139 | MsG0480023624.01.T01:CDS |
| AGATGTCATAAGATTATTAC+AGG | 0.349635 | 4:-87787348 | MsG0480023624.01.T01:CDS |
| TTTGGGACCCCACTTTAATC+TGG | 0.365824 | 4:+87787313 | None:intergenic |
| TCATTCCCTTATAATGGCTT+TGG | 0.367454 | 4:-87787133 | MsG0480023624.01.T01:CDS |
| TAGAATTGTTGCAATATTTG+TGG | 0.392340 | 4:+87787412 | None:intergenic |
| ATTACTAGCTTCTGGATGTT+GGG | 0.421769 | 4:-87787439 | MsG0480023624.01.T01:CDS |
| GAGTAACGATGTTCTAACTT+TGG | 0.426881 | 4:-87787175 | MsG0480023624.01.T01:CDS |
| AATTACTAGCTTCTGGATGT+TGG | 0.435257 | 4:-87787440 | MsG0480023624.01.T01:CDS |
| CTAACTTTGGATTTGGCGAC+TGG | 0.448700 | 4:-87787162 | MsG0480023624.01.T01:CDS |
| TTTCGGATAGTAAACAAAGA+TGG | 0.472848 | 4:-87787249 | MsG0480023624.01.T01:CDS |
| ATGGCTTTGGTAACGGTCTA+CGG | 0.484369 | 4:-87787120 | MsG0480023624.01.T01:CDS |
| CCTTATAATGGCTTTGGTAA+CGG | 0.486757 | 4:-87787127 | MsG0480023624.01.T01:CDS |
| TGTATAGAAAGAATCAGTAT+AGG | 0.493226 | 4:-87787285 | MsG0480023624.01.T01:CDS |
| ACGGTCTACGGTTTGATATG+TGG | 0.494396 | 4:-87787108 | MsG0480023624.01.T01:CDS |
| TCTACACTCCAATGGTTGTA+CGG | 0.500420 | 4:-87787577 | MsG0480023624.01.T01:CDS |
| ATCATAGACCGTACAACCAT+TGG | 0.508987 | 4:+87787569 | None:intergenic |
| CGTTACCAAAGCCATTATAA+GGG | 0.509599 | 4:+87787128 | None:intergenic |
| TTGTTCTTGAGGATAAACTT+TGG | 0.527814 | 4:+87787683 | None:intergenic |
| TGTACGGTCTATGATTCAAA+TGG | 0.529977 | 4:-87787561 | MsG0480023624.01.T01:CDS |
| CTAGCTTCTGGATGTTGGGA+AGG | 0.536127 | 4:-87787435 | MsG0480023624.01.T01:CDS |
| AATAGCTTGCCAGATTAAAG+TGG | 0.557737 | 4:-87787322 | MsG0480023624.01.T01:CDS |
| ATCGTTACTCAGAACAATCC+CGG | 0.565145 | 4:+87787188 | None:intergenic |
| TGGAAGAGAAGTGAATCTCA+TGG | 0.566929 | 4:-87787499 | MsG0480023624.01.T01:CDS |
| GTTGTGGATTCTTGTTCTTG+AGG | 0.567350 | 4:+87787672 | None:intergenic |
| TCACTTGTTCTACACTCCAA+TGG | 0.570907 | 4:-87787585 | MsG0480023624.01.T01:CDS |
| ATAGCTTGCCAGATTAAAGT+GGG | 0.573587 | 4:-87787321 | MsG0480023624.01.T01:CDS |
| GTGAGTCTCAGAGGATGTTG+TGG | 0.607959 | 4:+87787656 | None:intergenic |
| GTGAATCTCATGGATCTACA+AGG | 0.610519 | 4:-87787489 | MsG0480023624.01.T01:CDS |
| GAAGCCAAAAGGAACAACCA+TGG | 0.639127 | 4:-87787383 | MsG0480023624.01.T01:CDS |
| AGATGGACAAATCATTGCAG+AGG | 0.658249 | 4:-87787232 | MsG0480023624.01.T01:CDS |
| TGTTAGAGATGAGACACTGA+TGG | 0.668011 | 4:+87787634 | None:intergenic |
| ACACTGATGGTGAGTCTCAG+AGG | 0.681074 | 4:+87787647 | None:intergenic |
| GATGTCATAAGATTATTACA+GGG | 0.697573 | 4:-87787347 | MsG0480023624.01.T01:CDS |
| TAGCTTGCCAGATTAAAGTG+GGG | 0.739697 | 4:-87787320 | MsG0480023624.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TCTTTCTATACACAAATTTT+GGG | + | Chr4:87787492-87787511 | None:intergenic | 20.0% |
| !!! | TTCTTTCTATACACAAATTT+TGG | + | Chr4:87787493-87787512 | None:intergenic | 20.0% |
| ! | ACAAAGAGAAAAATACTGTA+TGG | - | Chr4:87787171-87787190 | MsG0480023624.01.T01:CDS | 25.0% |
| ! | AGATGTCATAAGATTATTAC+AGG | - | Chr4:87787437-87787456 | MsG0480023624.01.T01:CDS | 25.0% |
| ! | GATGTCATAAGATTATTACA+GGG | - | Chr4:87787438-87787457 | MsG0480023624.01.T01:CDS | 25.0% |
| ! | TAGAATTGTTGCAATATTTG+TGG | + | Chr4:87787376-87787395 | None:intergenic | 25.0% |
| ! | TGTATAGAAAGAATCAGTAT+AGG | - | Chr4:87787500-87787519 | MsG0480023624.01.T01:CDS | 25.0% |
| !! | AAAAAGAAATTACTAGCTTC+TGG | - | Chr4:87787338-87787357 | MsG0480023624.01.T01:CDS | 25.0% |
| !! | AGCTAGTAATTTCTTTTTGA+TGG | + | Chr4:87787336-87787355 | None:intergenic | 25.0% |
| !!! | GTTGATAACTATGACAAAAA+AGG | - | Chr4:87787263-87787282 | MsG0480023624.01.T01:CDS | 25.0% |
| !!! | TAGGAAAATCATTTGCTTTT+CGG | - | Chr4:87787519-87787538 | MsG0480023624.01.T01:CDS | 25.0% |
| !!! | TTCTAATTTTAGAAGCCAAA+AGG | - | Chr4:87787391-87787410 | MsG0480023624.01.T01:CDS | 25.0% |
| AAGCAAAAACAATCTTCATC+CGG | - | Chr4:87787578-87787597 | MsG0480023624.01.T01:CDS | 30.0% | |
| GATAACTATGACAAAAAAGG+TGG | - | Chr4:87787266-87787285 | MsG0480023624.01.T01:CDS | 30.0% | |
| TTGTTCTTGAGGATAAACTT+TGG | + | Chr4:87787105-87787124 | None:intergenic | 30.0% | |
| TTTCGGATAGTAAACAAAGA+TGG | - | Chr4:87787536-87787555 | MsG0480023624.01.T01:CDS | 30.0% | |
| AGCAAAAACAATCTTCATCC+GGG | - | Chr4:87787579-87787598 | MsG0480023624.01.T01:CDS | 35.0% | |
| CACAGATCATTCCCTTATAA+TGG | - | Chr4:87787646-87787665 | MsG0480023624.01.T01:CDS | 35.0% | |
| CGATGTTCTAACTTTGGATT+TGG | - | Chr4:87787616-87787635 | MsG0480023624.01.T01:CDS | 35.0% | |
| CGTTACCAAAGCCATTATAA+GGG | + | Chr4:87787660-87787679 | None:intergenic | 35.0% | |
| GAGTAACGATGTTCTAACTT+TGG | - | Chr4:87787610-87787629 | MsG0480023624.01.T01:CDS | 35.0% | |
| TCATTCCCTTATAATGGCTT+TGG | - | Chr4:87787652-87787671 | MsG0480023624.01.T01:CDS | 35.0% | |
| TGTACGGTCTATGATTCAAA+TGG | - | Chr4:87787224-87787243 | MsG0480023624.01.T01:CDS | 35.0% | |
| ! | AATAGCTTGCCAGATTAAAG+TGG | - | Chr4:87787463-87787482 | MsG0480023624.01.T01:CDS | 35.0% |
| ! | AATTACTAGCTTCTGGATGT+TGG | - | Chr4:87787345-87787364 | MsG0480023624.01.T01:CDS | 35.0% |
| ! | ATAGCTTGCCAGATTAAAGT+GGG | - | Chr4:87787464-87787483 | MsG0480023624.01.T01:CDS | 35.0% |
| ! | ATTACTAGCTTCTGGATGTT+GGG | - | Chr4:87787346-87787365 | MsG0480023624.01.T01:CDS | 35.0% |
| !! | CCTTATAATGGCTTTGGTAA+CGG | - | Chr4:87787658-87787677 | MsG0480023624.01.T01:CDS | 35.0% |
| !!! | CATCTTTTGACTTGAAACCA+TGG | + | Chr4:87787422-87787441 | None:intergenic | 35.0% |
| AGATGGACAAATCATTGCAG+AGG | - | Chr4:87787553-87787572 | MsG0480023624.01.T01:CDS | 40.0% | |
| ATCATAGACCGTACAACCAT+TGG | + | Chr4:87787219-87787238 | None:intergenic | 40.0% | |
| ATCGTTACTCAGAACAATCC+CGG | + | Chr4:87787600-87787619 | None:intergenic | 40.0% | |
| CCGTTACCAAAGCCATTATA+AGG | + | Chr4:87787661-87787680 | None:intergenic | 40.0% | |
| GTGAATCTCATGGATCTACA+AGG | - | Chr4:87787296-87787315 | MsG0480023624.01.T01:CDS | 40.0% | |
| GTTGTGGATTCTTGTTCTTG+AGG | + | Chr4:87787116-87787135 | None:intergenic | 40.0% | |
| TCACTTGTTCTACACTCCAA+TGG | - | Chr4:87787200-87787219 | MsG0480023624.01.T01:CDS | 40.0% | |
| TCTACACTCCAATGGTTGTA+CGG | - | Chr4:87787208-87787227 | MsG0480023624.01.T01:CDS | 40.0% | |
| TGGAAGAGAAGTGAATCTCA+TGG | - | Chr4:87787286-87787305 | MsG0480023624.01.T01:CDS | 40.0% | |
| TGTTAGAGATGAGACACTGA+TGG | + | Chr4:87787154-87787173 | None:intergenic | 40.0% | |
| ! | GAAACCATGGTTGTTCCTTT+TGG | + | Chr4:87787409-87787428 | None:intergenic | 40.0% |
| ! | TAGCTTGCCAGATTAAAGTG+GGG | - | Chr4:87787465-87787484 | MsG0480023624.01.T01:CDS | 40.0% |
| ACGGTCTACGGTTTGATATG+TGG | - | Chr4:87787677-87787696 | MsG0480023624.01.T01:CDS | 45.0% | |
| CTAACTTTGGATTTGGCGAC+TGG | - | Chr4:87787623-87787642 | MsG0480023624.01.T01:CDS | 45.0% | |
| GAAGCCAAAAGGAACAACCA+TGG | - | Chr4:87787402-87787421 | MsG0480023624.01.T01:CDS | 45.0% | |
| ! | TTTGGGACCCCACTTTAATC+TGG | + | Chr4:87787475-87787494 | None:intergenic | 45.0% |
| !! | ATGGCTTTGGTAACGGTCTA+CGG | - | Chr4:87787665-87787684 | MsG0480023624.01.T01:CDS | 45.0% |
| CTAGCTTCTGGATGTTGGGA+AGG | - | Chr4:87787350-87787369 | MsG0480023624.01.T01:CDS | 50.0% | |
| GTGAGTCTCAGAGGATGTTG+TGG | + | Chr4:87787132-87787151 | None:intergenic | 50.0% | |
| ! | ACACTGATGGTGAGTCTCAG+AGG | + | Chr4:87787141-87787160 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 87787098 | 87787709 | 87787098 | ID=MsG0480023624.01;Name=MsG0480023624.01 |
| Chr4 | mRNA | 87787098 | 87787709 | 87787098 | ID=MsG0480023624.01.T01;Parent=MsG0480023624.01;Name=MsG0480023624.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|203 |
| Chr4 | exon | 87787098 | 87787709 | 87787098 | ID=MsG0480023624.01.T01:exon:3251;Parent=MsG0480023624.01.T01 |
| Chr4 | CDS | 87787098 | 87787709 | 87787098 | ID=MsG0480023624.01.T01:cds;Parent=MsG0480023624.01.T01 |
| Gene Sequence |
| Protein sequence |
