AlfalfaGEDB | MsG0480023625.01

M. sativa cultivar ZhongmuNo.1 / MsG0480023625.01

KEGG: -
GO: -
PFAM: PF04525

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480023625.01.T01	XP_013447025.1	94.776	134	7	0	1	134	72	205	4.89E-87	262

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480023625.01.T01	Q9SH27	39.552	134	75	4	1	133	62	190	2.48E-22	90.9

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480023625.01.T01	A0A072TUE7	94.776	134	7	0	1	134	72	205	2.33e-87	262

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0480023625.01	MsG0480023718.01	0.804695	1.909670e-49	9.911852e-47
MsG0480023625.01	MsG0680030679.01	0.803335	3.661181e-49	1.834921e-46
MsG0480023625.01	MsG0780041787.01	0.800686	1.283037e-48	6.011695e-46

PPI

Gene1	Gene2	Type
MsG0780039359.01	MsG0480023625.01	PPI
MsG0780039368.01	MsG0480023625.01	PPI
MsG0780039372.01	MsG0480023625.01	PPI
MsG0780039372.01	MsG0480023625.01	PPI
MsG0780039370.01	MsG0480023625.01	PPI
MsG0480023624.01	MsG0480023625.01	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480023625.01.T01	MTR_8g097410	94.776	134	7	0	1	134	72	205	5.92e-91	262
MsG0480023625.01.T01	MTR_8g097390	89.552	134	14	0	1	134	67	200	4.46e-87	252
MsG0480023625.01.T01	MTR_8g097380	87.313	134	17	0	1	134	72	205	1.15e-83	244
MsG0480023625.01.T01	MTR_0415s0060	85.075	134	20	0	1	134	67	200	1.71e-82	241
MsG0480023625.01.T01	MTR_8g097400	85.075	134	20	0	1	134	72	205	2.35e-82	240
MsG0480023625.01.T01	MTR_8g097370	90.678	118	11	0	1	118	62	179	7.93e-77	227
MsG0480023625.01.T01	MTR_0559s0020	89.744	39	4	0	93	131	10	48	1.98e-17	71.6
MsG0480023625.01.T01	MTR_8g023060	30.714	140	86	4	1	134	65	199	1.68e-12	62.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480023625.01.T01	AT1G63410	39.259	135	76	4	1	134	62	191	1.37e-23	90.9
MsG0480023625.01.T01	AT1G63410	39.552	134	75	4	1	133	62	190	2.53e-23	90.9
MsG0480023625.01.T01	AT3G14260	31.159	138	84	5	1	130	92	226	2.38e-13	64.7
MsG0480023625.01.T01	AT1G53890	31.655	139	73	6	1	129	86	212	1.79e-11	59.7
MsG0480023625.01.T01	AT1G53870	31.655	139	73	6	1	129	86	212	1.79e-11	59.7
MsG0480023625.01.T01	AT1G53870	31.655	139	73	6	1	129	86	212	1.79e-11	59.7
MsG0480023625.01.T01	AT1G53890	31.655	139	73	6	1	129	86	212	1.79e-11	59.7

Find 29 sgRNAs with CRISPR-Local

Find 41 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTCTTTCTATGCACAAATTT+TGG	0.055275	4:+87793568	None:intergenic
CCGTAATCAGAGCTATTATT+AGG	0.235869	4:+87793400	None:intergenic
TTGCAGAGATTACTAGCTTT+TGG	0.275000	4:-87793720	MsG0480023625.01.T01:intron
ATAGCTCTGATTACGGTCTA+TGG	0.286716	4:-87793393	MsG0480023625.01.T01:CDS
AGATGTCATAAGGTTATATC+AGG	0.290968	4:-87793621	MsG0480023625.01.T01:CDS
TGATGTTCTAACTTTGGATT+TGG	0.311546	4:-87793442	MsG0480023625.01.T01:CDS
GTAAATCTCATGAATCTAAA+AGG	0.320119	4:-87793890	None:intergenic
CGTTCTCTGCACCATCAAAA+AGG	0.336589	4:-87793864	MsG0480023625.01.T01:intron
TTTGGGACCCCACTTTAATC+TGG	0.365824	4:+87793586	None:intergenic
TGAACCATGGTTGCTCCTCT+TGG	0.380118	4:+87793652	None:intergenic
TTTCGGATTGTAAACAAAGA+TGG	0.392530	4:-87793522	MsG0480023625.01.T01:CDS
CGTAATCAGAGCTATTATTA+GGG	0.393800	4:+87793401	None:intergenic
AAGTAATGATGTTCTAACTT+TGG	0.408274	4:-87793448	MsG0480023625.01.T01:CDS
CAAAGTTAAAAGATGTCATA+AGG	0.428313	4:-87793631	MsG0480023625.01.T01:CDS
CTAACTTTGGATTTGGCGGC+TGG	0.440479	4:-87793435	MsG0480023625.01.T01:CDS
TTTGGATTTGGCGGCTGGCA+CGG	0.445481	4:-87793430	MsG0480023625.01.T01:CDS
CCTAATAATAGCTCTGATTA+CGG	0.459361	4:-87793400	MsG0480023625.01.T01:CDS
TGTTCTAACTTTGGATTTGG+CGG	0.476974	4:-87793439	MsG0480023625.01.T01:CDS
ACGGTCTATGGTTTGATATG+CGG	0.535932	4:-87793381	MsG0480023625.01.T01:CDS
TGCATAGAAAGAATCAGTAT+AGG	0.542829	4:-87793558	MsG0480023625.01.T01:CDS
AATAGCTTGCCAGATTAAAG+TGG	0.557737	4:-87793595	MsG0480023625.01.T01:CDS
ATAGCTTGCCAGATTAAAGT+GGG	0.573587	4:-87793594	MsG0480023625.01.T01:CDS
GATGGACAAATCATTGCTGA+GGG	0.591065	4:-87793504	MsG0480023625.01.T01:CDS
TTCAAGTTTAAGAAGCCAAG+AGG	0.594213	4:-87793667	MsG0480023625.01.T01:CDS
AGATGGACAAATCATTGCTG+AGG	0.604214	4:-87793505	MsG0480023625.01.T01:CDS
GATGTCATAAGGTTATATCA+GGG	0.610635	4:-87793620	MsG0480023625.01.T01:CDS
ATCATTACTTAGAACAACCC+CGG	0.638115	4:+87793461	None:intergenic
GAAGCCAAGAGGAGCAACCA+TGG	0.643809	4:-87793656	MsG0480023625.01.T01:CDS
TAGCTTGCCAGATTAAAGTG+GGG	0.739697	4:-87793593	MsG0480023625.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	CTAATTAACTAATTAATGAA+TGG	+	Chr4:87793486-87793505	None:intergenic	15.0%
!	AAGTAATGATGTTCTAACTT+TGG	-	Chr4:87793804-87793823	MsG0480023625.01.T01:intron	25.0%
!	AATGGTTGATCAAAGATATA+TGG	+	Chr4:87793468-87793487	None:intergenic	25.0%
!	CAAAGTTAAAAGATGTCATA+AGG	-	Chr4:87793621-87793640	MsG0480023625.01.T01:CDS	25.0%
!!	TCTTTCTATGCACAAATTTT+GGG	+	Chr4:87793686-87793705	None:intergenic	25.0%
!!	TTCTTTCTATGCACAAATTT+TGG	+	Chr4:87793687-87793706	None:intergenic	25.0%
!!!	TAGGAAAATCATTTGCTTTT+CGG	-	Chr4:87793713-87793732	MsG0480023625.01.T01:CDS	25.0%
	AGATGTCATAAGGTTATATC+AGG	-	Chr4:87793631-87793650	MsG0480023625.01.T01:CDS	30.0%
	CGTAATCAGAGCTATTATTA+GGG	+	Chr4:87793854-87793873	None:intergenic	30.0%
	GATGTCATAAGGTTATATCA+GGG	-	Chr4:87793632-87793651	MsG0480023625.01.T01:CDS	30.0%
	TGATGTTCTAACTTTGGATT+TGG	-	Chr4:87793810-87793829	MsG0480023625.01.T01:intron	30.0%
	TGCATAGAAAGAATCAGTAT+AGG	-	Chr4:87793694-87793713	MsG0480023625.01.T01:CDS	30.0%
	TTTCGGATTGTAAACAAAGA+TGG	-	Chr4:87793730-87793749	MsG0480023625.01.T01:intron	30.0%
!	TGATGTAGATACCTTTTTGA+TGG	+	Chr4:87793402-87793421	None:intergenic	30.0%
!!	CCTAATAATAGCTCTGATTA+CGG	-	Chr4:87793852-87793871	MsG0480023625.01.T01:intron	30.0%
!!!	CATCTTTTAACTTTGAACCA+TGG	+	Chr4:87793616-87793635	None:intergenic	30.0%
	ATCATTACTTAGAACAACCC+CGG	+	Chr4:87793794-87793813	None:intergenic	35.0%
	CCGTAATCAGAGCTATTATT+AGG	+	Chr4:87793855-87793874	None:intergenic	35.0%
	TGTTCTAACTTTGGATTTGG+CGG	-	Chr4:87793813-87793832	MsG0480023625.01.T01:intron	35.0%
!	AATAGCTTGCCAGATTAAAG+TGG	-	Chr4:87793657-87793676	MsG0480023625.01.T01:CDS	35.0%
!	ATAGCTTGCCAGATTAAAGT+GGG	-	Chr4:87793658-87793677	MsG0480023625.01.T01:CDS	35.0%
!	TTCAAGTTTAAGAAGCCAAG+AGG	-	Chr4:87793585-87793604	MsG0480023625.01.T01:CDS	35.0%
!!!	ATTACTAGCTTTTGGATGCT+GGG	-	Chr4:87793540-87793559	MsG0480023625.01.T01:CDS	35.0%
!!!	TTGCAGAGATTACTAGCTTT+TGG	-	Chr4:87793532-87793551	MsG0480023625.01.T01:CDS	35.0%
	AAGCAAAAACAGTCGTCATC+CGG	-	Chr4:87793772-87793791	MsG0480023625.01.T01:intron	40.0%
	ACGGTCTATGGTTTGATATG+CGG	-	Chr4:87793871-87793890	MsG0480023625.01.T01:CDS	40.0%
	AGATGGACAAATCATTGCTG+AGG	-	Chr4:87793747-87793766	MsG0480023625.01.T01:intron	40.0%
	ATTAGCTATAGCTAGCAGAG+TGG	+	Chr4:87793427-87793446	None:intergenic	40.0%
	GATGGACAAATCATTGCTGA+GGG	-	Chr4:87793748-87793767	MsG0480023625.01.T01:intron	40.0%
!	TAGCTTGCCAGATTAAAGTG+GGG	-	Chr4:87793659-87793678	MsG0480023625.01.T01:CDS	40.0%
!!	ATAGCTCTGATTACGGTCTA+TGG	-	Chr4:87793859-87793878	MsG0480023625.01.T01:intron	40.0%
!!!	GATTACTAGCTTTTGGATGC+TGG	-	Chr4:87793539-87793558	MsG0480023625.01.T01:CDS	40.0%
	AGCAAAAACAGTCGTCATCC+GGG	-	Chr4:87793773-87793792	MsG0480023625.01.T01:intron	45.0%
	CGTTCTCTGCACCATCAAAA+AGG	-	Chr4:87793388-87793407	MsG0480023625.01.T01:CDS	45.0%
!	TTTGGGACCCCACTTTAATC+TGG	+	Chr4:87793669-87793688	None:intergenic	45.0%
	CTAACTTTGGATTTGGCGGC+TGG	-	Chr4:87793817-87793836	MsG0480023625.01.T01:intron	50.0%
	GCAAAAACAGTCGTCATCCG+GGG	-	Chr4:87793774-87793793	MsG0480023625.01.T01:intron	50.0%
	TGAACCATGGTTGCTCCTCT+TGG	+	Chr4:87793603-87793622	None:intergenic	50.0%
!!	CTAGCTTTTGGATGCTGGGA+AGG	-	Chr4:87793544-87793563	MsG0480023625.01.T01:CDS	50.0%
	GAAGCCAAGAGGAGCAACCA+TGG	-	Chr4:87793596-87793615	MsG0480023625.01.T01:CDS	55.0%
	TTTGGATTTGGCGGCTGGCA+CGG	-	Chr4:87793822-87793841	MsG0480023625.01.T01:intron	55.0%

Chromosome	Type	Strat	End	Strand	Name
Chr4	gene	87793371	87793903	87793371	ID=MsG0480023625.01;Name=MsG0480023625.01
Chr4	mRNA	87793371	87793903	87793371	ID=MsG0480023625.01.T01;Parent=MsG0480023625.01;Name=MsG0480023625.01.T01;_AED=0.50;_eAED=0.50;_QI=0\|0\|0\|1\|0\|0\|2\|0\|134
Chr4	exon	87793371	87793736	87793371	ID=MsG0480023625.01.T01:exon:3312;Parent=MsG0480023625.01.T01
Chr4	exon	87793865	87793903	87793865	ID=MsG0480023625.01.T01:exon:3311;Parent=MsG0480023625.01.T01
Chr4	CDS	87793865	87793903	87793865	ID=MsG0480023625.01.T01:cds;Parent=MsG0480023625.01.T01
Chr4	CDS	87793371	87793736	87793371	ID=MsG0480023625.01.T01:cds;Parent=MsG0480023625.01.T01

Gene Sequence

>MsG0480023625.01.T01

ATGAATCTAAAAGGAAACGTTCTCTGCACCATCAAAAAGAGATTACTAGCTTTTGGATGCTGGGAAGGACACAAACATTGCAACAATTCAAGTTTAAGAAGCCAAGAGGAGCAACCATGGTTCAAAGTTAAAAGATGTCATAAGGTTATATCAGGGAAAATAGCTTGCCAGATTAAAGTGGGGTCCCAAAATTTGTGCATAGAAAGAATCAGTATAGGAAAATCATTTGCTTTTCGGATTGTAAACAAAGATGGACAAATCATTGCTGAGGGAAAGCAAAAACAGTCGTCATCCGGGGTTGTTCTAAGTAATGATGTTCTAACTTTGGATTTGGCGGCTGGCACGGATCATTCCCTAATAATAGCTCTGATTACGGTCTATGGTTTGATATGCGGCTTAATGTAG

Protein sequence

>MsG0480023625.01.T01

MNLKGNVLCTIKKRLLAFGCWEGHKHCNNSSLRSQEEQPWFKVKRCHKVISGKIACQIKVGSQNLCIERISIGKSFAFRIVNKDGQIIAEGKQKQSSSGVVLSNDVLTLDLAAGTDHSLIIALITVYGLICGLM*