AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780039370.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780039370.01.T01 MTR_7g072120 82.857 210 28 4 1 202 1 210 4.49e-128 359
MsG0780039370.01.T01 MTR_7g072170 80.476 210 33 2 1 202 1 210 9.37e-125 352
MsG0780039370.01.T01 MTR_7g072270 79.710 207 36 1 1 201 1 207 1.71e-124 350
MsG0780039370.01.T01 MTR_7g072140 79.048 210 36 2 1 202 1 210 8.46e-123 346
MsG0780039370.01.T01 MTR_7g072250 67.742 217 49 3 1 200 25 237 9.87e-101 291
MsG0780039370.01.T01 MTR_7g072270 78.788 165 29 1 1 159 1 165 1.98e-96 278
MsG0780039370.01.T01 MTR_7g071710 96.610 118 4 0 86 203 1 118 6.66e-82 239
MsG0780039370.01.T01 MTR_4g029430 56.853 197 80 2 6 201 5 197 8.30e-80 237
MsG0780039370.01.T01 MTR_1g014180 52.151 186 79 5 16 196 5 185 7.60e-59 183
MsG0780039370.01.T01 MTR_1g014150 46.739 184 90 4 14 195 11 188 3.30e-47 154
MsG0780039370.01.T01 MTR_1g014160 40.426 188 87 6 26 195 59 239 3.08e-30 111
MsG0780039370.01.T01 MTR_2g103840 31.138 167 107 5 39 201 42 204 4.67e-23 92.4
MsG0780039370.01.T01 MTR_5g074550 31.111 180 109 6 26 195 17 191 2.78e-21 87.4
MsG0780039370.01.T01 MTR_4g010180 26.738 187 130 6 20 202 11 194 1.95e-20 85.5
MsG0780039370.01.T01 MTR_2g103850 29.762 168 110 5 39 202 43 206 1.10e-19 83.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780039370.01.T01 AT5G01750 57.500 200 84 1 4 202 15 214 4.97e-92 268
MsG0780039370.01.T01 AT3G11740 58.947 190 76 2 15 202 2 191 8.53e-83 244
MsG0780039370.01.T01 AT2G14560 51.813 193 91 2 9 200 1 192 1.49e-71 216
MsG0780039370.01.T01 AT2G14560 51.020 196 94 2 6 200 58 252 1.78e-70 215
MsG0780039370.01.T01 AT1G33840 46.919 211 100 2 1 199 1 211 4.15e-68 208
MsG0780039370.01.T01 AT5G01750 55.696 158 69 1 4 160 15 172 5.00e-67 203
MsG0780039370.01.T01 AT2G14560 48.780 164 82 2 9 171 1 163 1.67e-55 174
MsG0780039370.01.T01 AT2G14560 49.682 157 77 2 6 161 58 213 1.57e-54 174
MsG0780039370.01.T01 AT1G33840 43.353 173 86 2 1 161 1 173 4.91e-50 160
MsG0780039370.01.T01 AT3G16900 42.703 185 86 4 16 199 15 180 1.06e-49 160
MsG0780039370.01.T01 AT3G56180 35.567 194 118 4 12 201 14 204 9.78e-37 127
MsG0780039370.01.T01 AT3G56180 35.052 194 117 5 12 201 14 202 2.09e-34 121
MsG0780039370.01.T01 AT3G10986 32.632 190 121 4 15 197 3 192 8.65e-30 109
MsG0780039370.01.T01 AT3G15810 31.548 168 105 6 39 202 44 205 3.88e-24 95.5
MsG0780039370.01.T01 AT1G80120 33.333 168 105 4 39 202 30 194 1.05e-19 83.6
MsG0780039370.01.T01 AT2G38640 31.953 169 106 5 33 198 30 192 7.15e-17 75.5
MsG0780039370.01.T01 AT5G20640 28.177 181 113 5 36 201 33 211 3.58e-15 71.6
MsG0780039370.01.T01 AT2G30270 28.324 173 109 7 26 195 19 179 8.10e-15 69.7
MsG0780039370.01.T01 AT2G05910 29.586 169 98 8 39 198 28 184 1.23e-13 67.0
MsG0780039370.01.T01 AT5G41590 23.864 176 124 4 33 198 44 219 5.00e-11 60.1

Find 51 sgRNAs with CRISPR-Local

Find 68 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAATCACAAACATCTTCTT+TGG 0.257604 7:-62922924 None:intergenic
GGATTTAGCGGTCGTGAAAA+AGG 0.322249 7:+62922007 MsG0780039370.01.T01:CDS
GCACCCACCACCACCATATT+CGG 0.351246 7:+62921957 MsG0780039370.01.T01:CDS
ACAATATTGAGAGCCGAATA+TGG 0.363340 7:-62921970 None:intergenic
TAGGGTGGTGATGGGGTTAC+CGG 0.377318 7:-62922141 None:intergenic
GTTGCCATTGACATCTTTAA+CGG 0.392620 7:-62922054 None:intergenic
AATATGGTGGTGGTGGGTGC+TGG 0.394560 7:-62921954 None:intergenic
ACCGCTAAATCCACCGGATA+AGG 0.395830 7:-62921996 None:intergenic
AGCTAGAAACACATCTAGTT+TGG 0.401309 7:-62922894 None:intergenic
TCAATATTGTGCTCCTTATC+CGG 0.417584 7:+62921983 MsG0780039370.01.T01:CDS
CCTCCGTGACCGCCGTGTCT+TGG 0.425443 7:+62922109 MsG0780039370.01.T01:CDS
GTGGTGGTGGGTGCTGGCAT+CGG 0.428409 7:-62921948 None:intergenic
CTTGCGACGTAGGGTGGTGA+TGG 0.443247 7:-62922150 None:intergenic
GCTGGCATCGGTGGTTGATA+AGG 0.448493 7:-62921936 None:intergenic
TCCTTATCCGGTGGATTTAG+CGG 0.450209 7:+62921995 MsG0780039370.01.T01:CDS
TTGCGACGTAGGGTGGTGAT+GGG 0.485246 7:-62922149 None:intergenic
CGATCTTGTATTGTTTATGC+CGG 0.493058 7:+62922971 MsG0780039370.01.T01:CDS
CGTGTCTTGGTTGATGCCTC+CGG 0.494853 7:+62922122 MsG0780039370.01.T01:CDS
GTGGTGGGTGCTGGCATCGG+TGG 0.497728 7:-62921945 None:intergenic
ATGAAAGCGTAATCAACATT+GGG 0.498374 7:-62923202 None:intergenic
TAAAGTCAAAGGTAGTTGGT+TGG 0.499063 7:+62922946 MsG0780039370.01.T01:CDS
AACAATGTTGTTAGTATCAC+CGG 0.501905 7:-62922990 None:intergenic
TATGAAAGCGTAATCAACAT+TGG 0.519673 7:-62923203 None:intergenic
AAACTAGATGTGTTTCTAGC+TGG 0.525823 7:+62922896 MsG0780039370.01.T01:CDS
CAACCAAGACACGGCGGTCA+CGG 0.526860 7:-62922112 None:intergenic
GATAGACAGTGACTGCGAAA+TGG 0.528270 7:-62923180 None:intergenic
AACGGTGAAGTTGTCTGCCA+TGG 0.529412 7:-62922036 None:intergenic
TACTAACAACATTGTTGCAC+AGG 0.530405 7:+62922997 MsG0780039370.01.T01:CDS
TGTTGATTACGCTTTCATAG+TGG 0.534696 7:+62923207 MsG0780039370.01.T01:CDS
TTCACGACCGCTAAATCCAC+CGG 0.537903 7:-62922002 None:intergenic
AACGTCGTGTTTAAAGTGAA+AGG 0.567690 7:+62922074 MsG0780039370.01.T01:CDS
AGAGCCGAATATGGTGGTGG+TGG 0.571753 7:-62921961 None:intergenic
TGTACATACCTTGCGACGTA+GGG 0.572581 7:-62922159 None:intergenic
AGTGACTATGCATGATCGGT+GGG 0.580863 7:+62922793 MsG0780039370.01.T01:CDS
ATATTGTGCTCCTTATCCGG+TGG 0.590508 7:+62921986 MsG0780039370.01.T01:CDS
CATCACCACCCTACGTCGCA+AGG 0.590882 7:+62922151 MsG0780039370.01.T01:CDS
TTCACCGTTAAAGATGTCAA+TGG 0.593126 7:+62922050 MsG0780039370.01.T01:CDS
CCAAGACACGGCGGTCACGG+AGG 0.598529 7:-62922109 None:intergenic
CAAGACACGGCGGTCACGGA+GGG 0.598937 7:-62922108 None:intergenic
TAGTGACTATGCATGATCGG+TGG 0.614019 7:+62922792 MsG0780039370.01.T01:CDS
GGTGGTGATGGGGTTACCGG+AGG 0.620164 7:-62922138 None:intergenic
ACATACCTTGCGACGTAGGG+TGG 0.638701 7:-62922156 None:intergenic
TTGTACATACCTTGCGACGT+AGG 0.644546 7:-62922160 None:intergenic
AGATAGTGACTATGCATGAT+CGG 0.644746 7:+62922789 MsG0780039370.01.T01:intron
TGCGACGTAGGGTGGTGATG+GGG 0.652061 7:-62922148 None:intergenic
CGGAGGCATCAACCAAGACA+CGG 0.654823 7:-62922121 None:intergenic
TTGAGAGCCGAATATGGTGG+TGG 0.662726 7:-62921964 None:intergenic
GAGCCGAATATGGTGGTGGT+GGG 0.669874 7:-62921960 None:intergenic
GATCGGTGGGAAGCTTACAG+AGG 0.712766 7:+62922806 MsG0780039370.01.T01:CDS
ATATTGAGAGCCGAATATGG+TGG 0.741009 7:-62921967 None:intergenic
AGGCATCAACCAAGACACGG+CGG 0.796971 7:-62922118 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTATCAACTAAAAAAAATA+TGG + Chr7:62922580-62922599 MsG0780039370.01.T01:intron 10.0%
!!! TTATTTTTTAAATAAAGAGT+AGG + Chr7:62922271-62922290 MsG0780039370.01.T01:intron 10.0%
!! AGTATGTGAATTTCAATAAT+TGG + Chr7:62922622-62922641 MsG0780039370.01.T01:intron 20.0%
!! TTGAGATATATGAAGTATAA+TGG - Chr7:62922727-62922746 None:intergenic 20.0%
!!! ATATAAGGTTTTACTTTGAT+TGG + Chr7:62923051-62923070 MsG0780039370.01.T01:intron 20.0%
!!! TGCTCAATATTTTAATTTTG+TGG + Chr7:62922221-62922240 MsG0780039370.01.T01:intron 20.0%
!!! TGTAAATTCAAGAGTATATA+AGG + Chr7:62923036-62923055 MsG0780039370.01.T01:intron 20.0%
! AAAATCACAAACATCTTCTT+TGG - Chr7:62922927-62922946 None:intergenic 25.0%
! TTTGATTGGATTATCACTTA+AGG + Chr7:62923065-62923084 MsG0780039370.01.T01:intron 25.0%
!! GATTCATTAAGTTGTACTAT+TGG + Chr7:62922763-62922782 MsG0780039370.01.T01:intron 25.0%
!!! AAAAATATGGTTTTGCTCAA+TGG + Chr7:62922593-62922612 MsG0780039370.01.T01:intron 25.0%
!!! ATAACTTTTTTTTTTCGTGC+AGG - Chr7:62922558-62922577 None:intergenic 25.0%
!!! ATTTTAAAGTCAAAGGTAGT+TGG + Chr7:62922942-62922961 MsG0780039370.01.T01:CDS 25.0%
!!! GTTTGTGATTTTAAAGTCAA+AGG + Chr7:62922935-62922954 MsG0780039370.01.T01:CDS 25.0%
AACAATGTTGTTAGTATCAC+CGG - Chr7:62922993-62923012 None:intergenic 30.0%
! ATGAAAGCGTAATCAACATT+GGG - Chr7:62923205-62923224 None:intergenic 30.0%
! TATGAAAGCGTAATCAACAT+TGG - Chr7:62923206-62923225 None:intergenic 30.0%
ACAATATTGAGAGCCGAATA+TGG - Chr7:62921973-62921992 None:intergenic 35.0%
AGATAGTGACTATGCATGAT+CGG + Chr7:62922789-62922808 MsG0780039370.01.T01:intron 35.0%
AGCTAGAAACACATCTAGTT+TGG - Chr7:62922897-62922916 None:intergenic 35.0%
CGATCTTGTATTGTTTATGC+CGG + Chr7:62922971-62922990 MsG0780039370.01.T01:CDS 35.0%
GTTGCCATTGACATCTTTAA+CGG - Chr7:62922057-62922076 None:intergenic 35.0%
TAAAGTCAAAGGTAGTTGGT+TGG + Chr7:62922946-62922965 MsG0780039370.01.T01:CDS 35.0%
TACTAACAACATTGTTGCAC+AGG + Chr7:62922997-62923016 MsG0780039370.01.T01:CDS 35.0%
TCAATATTGTGCTCCTTATC+CGG + Chr7:62921983-62922002 MsG0780039370.01.T01:CDS 35.0%
TTCACCGTTAAAGATGTCAA+TGG + Chr7:62922050-62922069 MsG0780039370.01.T01:CDS 35.0%
! AACGTCGTGTTTAAAGTGAA+AGG + Chr7:62922074-62922093 MsG0780039370.01.T01:CDS 35.0%
! TGTTGATTACGCTTTCATAG+TGG + Chr7:62923207-62923226 MsG0780039370.01.T01:CDS 35.0%
!! AAACTAGATGTGTTTCTAGC+TGG + Chr7:62922896-62922915 MsG0780039370.01.T01:CDS 35.0%
ATATTGAGAGCCGAATATGG+TGG - Chr7:62921970-62921989 None:intergenic 40.0%
CGTGAAAAAGGTAATGACCA+TGG + Chr7:62922019-62922038 MsG0780039370.01.T01:CDS 40.0%
AGTGACTATGCATGATCGGT+GGG + Chr7:62922793-62922812 MsG0780039370.01.T01:CDS 45.0%
ATATTGTGCTCCTTATCCGG+TGG + Chr7:62921986-62922005 MsG0780039370.01.T01:CDS 45.0%
GATAGACAGTGACTGCGAAA+TGG - Chr7:62923183-62923202 None:intergenic 45.0%
GGATTTAGCGGTCGTGAAAA+AGG + Chr7:62922007-62922026 MsG0780039370.01.T01:CDS 45.0%
TAGTGACTATGCATGATCGG+TGG + Chr7:62922792-62922811 MsG0780039370.01.T01:CDS 45.0%
TGTACATACCTTGCGACGTA+GGG - Chr7:62922162-62922181 None:intergenic 45.0%
TTGTACATACCTTGCGACGT+AGG - Chr7:62922163-62922182 None:intergenic 45.0%
! TCCTTATCCGGTGGATTTAG+CGG + Chr7:62921995-62922014 MsG0780039370.01.T01:CDS 45.0%
!!! TTTACTTTTTTTTTTTTTTT+TGG - Chr7:62922659-62922678 None:intergenic 5.0%
AACGGTGAAGTTGTCTGCCA+TGG - Chr7:62922039-62922058 None:intergenic 50.0%
ACCGCTAAATCCACCGGATA+AGG - Chr7:62921999-62922018 None:intergenic 50.0%
CAACCACGTGAGTCTACTCT+CGG - Chr7:62922301-62922320 None:intergenic 50.0%
TTCACGACCGCTAAATCCAC+CGG - Chr7:62922005-62922024 None:intergenic 50.0%
TTGAGAGCCGAATATGGTGG+TGG - Chr7:62921967-62921986 None:intergenic 50.0%
ACATACCTTGCGACGTAGGG+TGG - Chr7:62922159-62922178 None:intergenic 55.0%
AGGCATCAACCAAGACACGG+CGG - Chr7:62922121-62922140 None:intergenic 55.0%
CATCCGAGAGTAGACTCACG+TGG + Chr7:62922295-62922314 MsG0780039370.01.T01:intron 55.0%
GATCGGTGGGAAGCTTACAG+AGG + Chr7:62922806-62922825 MsG0780039370.01.T01:CDS 55.0%
GCACCCACCACCACCATATT+CGG + Chr7:62921957-62921976 MsG0780039370.01.T01:CDS 55.0%
TTGCGACGTAGGGTGGTGAT+GGG - Chr7:62922152-62922171 None:intergenic 55.0%
! CGTGTCTTGGTTGATGCCTC+CGG + Chr7:62922122-62922141 MsG0780039370.01.T01:CDS 55.0%
!! AATATGGTGGTGGTGGGTGC+TGG - Chr7:62921957-62921976 None:intergenic 55.0%
!! AGAGCCGAATATGGTGGTGG+TGG - Chr7:62921964-62921983 None:intergenic 55.0%
!! CGGAGGCATCAACCAAGACA+CGG - Chr7:62922124-62922143 None:intergenic 55.0%
!! GAGCCGAATATGGTGGTGGT+GGG - Chr7:62921963-62921982 None:intergenic 55.0%
!! GCTGGCATCGGTGGTTGATA+AGG - Chr7:62921939-62921958 None:intergenic 55.0%
!! TAGGGTGGTGATGGGGTTAC+CGG - Chr7:62922144-62922163 None:intergenic 55.0%
CAACCAAGACACGGCGGTCA+CGG - Chr7:62922115-62922134 None:intergenic 60.0%
CATCACCACCCTACGTCGCA+AGG + Chr7:62922151-62922170 MsG0780039370.01.T01:CDS 60.0%
CTTGCGACGTAGGGTGGTGA+TGG - Chr7:62922153-62922172 None:intergenic 60.0%
! TGCGACGTAGGGTGGTGATG+GGG - Chr7:62922151-62922170 None:intergenic 60.0%
CAAGACACGGCGGTCACGGA+GGG - Chr7:62922111-62922130 None:intergenic 65.0%
!! GGTGGTGATGGGGTTACCGG+AGG - Chr7:62922141-62922160 None:intergenic 65.0%
!! GTGGTGGTGGGTGCTGGCAT+CGG - Chr7:62921951-62921970 None:intergenic 65.0%
CCAAGACACGGCGGTCACGG+AGG - Chr7:62922112-62922131 None:intergenic 70.0%
CCTCCGTGACCGCCGTGTCT+TGG + Chr7:62922109-62922128 MsG0780039370.01.T01:CDS 70.0%
!! GTGGTGGGTGCTGGCATCGG+TGG - Chr7:62921948-62921967 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
Chr7 gene 62921927 62923273 62921927 ID=MsG0780039370.01;Name=MsG0780039370.01
Chr7 mRNA 62921927 62923273 62921927 ID=MsG0780039370.01.T01;Parent=MsG0780039370.01;Name=MsG0780039370.01.T01;_AED=0.40;_eAED=0.40;_QI=0|0|0|1|1|1|3|0|203
Chr7 exon 62921927 62922172 62921927 ID=MsG0780039370.01.T01:exon:16921;Parent=MsG0780039370.01.T01
Chr7 exon 62922791 62923018 62922791 ID=MsG0780039370.01.T01:exon:16920;Parent=MsG0780039370.01.T01
Chr7 exon 62923136 62923273 62923136 ID=MsG0780039370.01.T01:exon:16919;Parent=MsG0780039370.01.T01
Chr7 CDS 62921927 62922172 62921927 ID=MsG0780039370.01.T01:cds;Parent=MsG0780039370.01.T01
Chr7 CDS 62922791 62923018 62922791 ID=MsG0780039370.01.T01:cds;Parent=MsG0780039370.01.T01
Chr7 CDS 62923136 62923273 62923136 ID=MsG0780039370.01.T01:cds;Parent=MsG0780039370.01.T01
Gene Sequence

>MsG0780039370.01.T01

ATGGCTTATCCTTATCAACCACCGATGCCAGCACCCACCACCACCATATTCGGCTCTCAATATTGTGCTCCTTATCCGGTGGATTTAGCGGTCGTGAAAAAGGTAATGACCATGGCAGACAACTTCACCGTTAAAGATGTCAATGGCAACGTCGTGTTTAAAGTGAAAGGTTCTCTCTTAACCCTCCGTGACCGCCGTGTCTTGGTTGATGCCTCCGGTAACCCCATCACCACCCTACGTCGCAAGATAGTGACTATGCATGATCGGTGGGAAGCTTACAGAGGAGAAAGCACAAATTCTAAAGATCTTATATTTACATTGAGAAAATCATCGCTATTGCAGTTGAAGACCAAACTAGATGTGTTTCTAGCTGGTAATACCAAAGAAGATGTTTGTGATTTTAAAGTCAAAGGTAGTTGGTTGGAACGATCTTGTATTGTTTATGCCGGTGATACTAACAACATTGTTGCACAGATGCACAAAAAGCACACTATTCAGAGTATTTTAATTGATAAAGACCATTTCGCAGTCACTGTCTATCCCAATGTTGATTACGCTTTCATAGTGGCATTAATCGCGATTCTTAACGAGATTAACGAAGATGATGATTGA

Protein sequence

>MsG0780039370.01.T01

MAYPYQPPMPAPTTTIFGSQYCAPYPVDLAVVKKVMTMADNFTVKDVNGNVVFKVKGSLLTLRDRRVLVDASGNPITTLRRKIVTMHDRWEAYRGESTNSKDLIFTLRKSSLLQLKTKLDVFLAGNTKEDVCDFKVKGSWLERSCIVYAGDTNNIVAQMHKKHTIQSILIDKDHFAVTVYPNVDYAFIVALIAILNEINEDDD*