AlfalfaGEDB | MsG0480023642.01

M. sativa cultivar ZhongmuNo.1 / MsG0480023642.01

KEGG: -
GO: GO:0003682 GO:0006351
PFAM: PF01426 PF07500

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480023642.01.T01	GAV57952.1	47.908	478	143	8	23	401	90	560	9.37E-135	410

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480023642.01.T01	G7LHC0	83.427	356	15	2	30	341	69	424	0.0	596

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180000210.01	MsG0480023642.01	0.810348	1.205108e-50	7.246933e-48
MsG0180005923.01	MsG0480023642.01	0.802992	4.311639e-49	2.141969e-46
MsG0280006469.01	MsG0480023642.01	0.828744	7.582432e-55	7.603206e-52
MsG0280007955.01	MsG0480023642.01	0.818029	2.426067e-52	1.795559e-49
MsG0380015555.01	MsG0480023642.01	0.803939	2.743669e-49	1.396719e-46
MsG0380016163.01	MsG0480023642.01	0.810013	1.423133e-50	8.484004e-48
MsG0480019181.01	MsG0480023642.01	0.839525	1.504148e-57	2.082604e-54
MsG0480023489.01	MsG0480023642.01	0.809752	1.620180e-50	9.592657e-48
MsG0480023642.01	MsG0780041662.01	0.809704	1.659234e-50	9.810911e-48
MsG0480023642.01	MsG0880045453.01	0.801394	9.193940e-49	4.385707e-46

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480023642.01.T01	MTR_8g098290	83.427	356	15	2	30	341	69	424	0.0	596
MsG0480023642.01.T01	MTR_8g098290	98.571	70	1	0	342	411	502	571	1.04e-38	147
MsG0480023642.01.T01	MTR_8g098290	83.193	357	16	2	30	342	69	425	0.0	595
MsG0480023642.01.T01	MTR_5g010190	57.337	368	106	7	23	341	57	422	3.26e-133	391
MsG0480023642.01.T01	MTR_5g010190	57.337	368	106	7	23	341	57	422	6.50e-132	392
MsG0480023642.01.T01	MTR_5g010190	61.667	60	22	1	341	399	499	558	1.54e-14	76.3
MsG0480023642.01.T01	MTR_8g098260	61.905	357	89	5	30	341	47	401	6.89e-128	400
MsG0480023642.01.T01	MTR_8g098260	72.727	66	14	2	342	404	479	543	1.05e-19	92.8
MsG0480023642.01.T01	MTR_6g464540	48.287	321	111	9	34	342	50	327	2.70e-85	265

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480023642.01.T01	AT4G11560	46.429	392	155	7	32	381	99	477	6.00e-108	328
MsG0480023642.01.T01	AT4G11560	46.429	392	155	7	32	381	99	477	8.89e-107	328
MsG0480023642.01.T01	AT2G25120	38.329	347	165	8	19	349	48	361	4.65e-54	185
MsG0480023642.01.T01	AT4G23120	40.060	332	152	11	27	341	22	323	2.88e-49	171
MsG0480023642.01.T01	AT3G43990	52.239	134	63	1	27	160	47	179	7.65e-40	147
MsG0480023642.01.T01	AT3G48050	38.000	100	58	3	60	159	47	142	3.51e-11	66.2
MsG0480023642.01.T01	AT3G48050	38.000	100	58	3	60	159	47	142	3.51e-11	66.2
MsG0480023642.01.T01	AT3G48060	38.000	100	58	3	60	159	47	142	3.86e-11	65.9
MsG0480023642.01.T01	AT3G48060	38.000	100	58	3	60	159	47	142	3.86e-11	65.9
MsG0480023642.01.T01	AT3G48060	38.000	100	58	3	60	159	48	143	3.86e-11	65.9

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Find 147 sgRNAs with CRISPR-Local

Find 477 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACAGCTCTCTGGTGTCAA+CGG	0.576675	4:-88015407	None:intergenic
AAACCTAACGGGTTCACCAA+TGG	0.548184	4:-88014182	None:intergenic
AAAGTGCTTAAAGAGTGCCC+GGG	0.594463	4:-88019718	None:intergenic
AAATAGAAGAAACTTTAAGA+AGG	0.497072	4:+88015719	MsG0480023642.01.T01:CDS
AAATGGATGTTGACTTGACG+AGG	0.657204	4:+88019662	MsG0480023642.01.T01:three_prime_UTR
AAATGTAGGCACGGCCCCAT+GGG	0.524078	4:+88019319	MsG0480023642.01.T01:CDS
AAATTTCGCAGTTGCCCATG+GGG	0.641672	4:-88019333	None:intergenic
AACCCATTGGTGAACCCGTT+AGG	0.478741	4:+88014179	MsG0480023642.01.T01:CDS
AACCTAACGGGTTCACCAAT+GGG	0.437811	4:-88014181	None:intergenic
AAGGGCTGAGATTGCCTGAC+AGG	0.434084	4:-88019786	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AACAATGTGACGGTTAATAA+TGG	-	Chr4:88014865-88014884	None:intergenic	30.0%
AACACCGTTTGACAAAAAAT+TGG	+	Chr4:88018273-88018292	MsG0480023642.01.T01:intron	30.0%
AACTCATCACTGAACTTAAA+GGG	-	Chr4:88016811-88016830	None:intergenic	30.0%
AAGAAATCACAACCACAATT+GGG	-	Chr4:88016079-88016098	None:intergenic	30.0%
ACACAATCCATAAATGGAAA+TGG	+	Chr4:88015310-88015329	MsG0480023642.01.T01:CDS	30.0%
ACACAGATGAATTGAAATTG+TGG	+	Chr4:88015560-88015579	MsG0480023642.01.T01:intron	30.0%
AGCCTGTAAGTAGTATAATA+CGG	-	Chr4:88017114-88017133	None:intergenic	30.0%
ATTATGGATATTTGAACTCC+AGG	+	Chr4:88018883-88018902	MsG0480023642.01.T01:intron	30.0%
ATTGGAAATGGAAATGGAAA+TGG	-	Chr4:88013907-88013926	None:intergenic	30.0%
ATTTGGAAGTTGTTTGTGTA+TGG	-	Chr4:88015500-88015519	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr4	gene	88013758	88020323	88013758	ID=MsG0480023642.01;Name=MsG0480023642.01
Chr4	mRNA	88013758	88020323	88013758	ID=MsG0480023642.01.T01;Parent=MsG0480023642.01;Name=MsG0480023642.01.T01;_AED=0.50;_eAED=0.50;_QI=318\|0.4\|0.5\|1\|1\|1\|6\|809\|434
Chr4	exon	88013758	88014273	88013758	ID=MsG0480023642.01.T01:exon:6206;Parent=MsG0480023642.01.T01
Chr4	exon	88014459	88014521	88014459	ID=MsG0480023642.01.T01:exon:6207;Parent=MsG0480023642.01.T01
Chr4	exon	88015304	88015525	88015304	ID=MsG0480023642.01.T01:exon:6208;Parent=MsG0480023642.01.T01
Chr4	exon	88015642	88016021	88015642	ID=MsG0480023642.01.T01:exon:6209;Parent=MsG0480023642.01.T01
Chr4	exon	88016169	88016328	88016169	ID=MsG0480023642.01.T01:exon:6210;Parent=MsG0480023642.01.T01
Chr4	exon	88019233	88020323	88019233	ID=MsG0480023642.01.T01:exon:6211;Parent=MsG0480023642.01.T01
Chr4	five_prime_UTR	88013758	88014075	88013758	ID=MsG0480023642.01.T01:five_prime_utr;Parent=MsG0480023642.01.T01
Chr4	CDS	88014076	88014273	88014076	ID=MsG0480023642.01.T01:cds;Parent=MsG0480023642.01.T01
Chr4	CDS	88014459	88014521	88014459	ID=MsG0480023642.01.T01:cds;Parent=MsG0480023642.01.T01
Chr4	CDS	88015304	88015525	88015304	ID=MsG0480023642.01.T01:cds;Parent=MsG0480023642.01.T01
Chr4	CDS	88015642	88016021	88015642	ID=MsG0480023642.01.T01:cds;Parent=MsG0480023642.01.T01
Chr4	CDS	88016169	88016328	88016169	ID=MsG0480023642.01.T01:cds;Parent=MsG0480023642.01.T01
Chr4	CDS	88019233	88019514	88019233	ID=MsG0480023642.01.T01:cds;Parent=MsG0480023642.01.T01
Chr4	three_prime_UTR	88019515	88020323	88019515	ID=MsG0480023642.01.T01:three_prime_utr;Parent=MsG0480023642.01.T01

Gene Sequence

>MsG0480023642.01.T01

ATGAAGCCGAGTGACGAGGCTGAGAAGGAGGAAGAAGAGGAACAGTCCAGCGAAGAAGAAGAAGAAGAAGAAGAAGAGGAAGAATCTCCAGTTGAAGACGCTAAACCCATTGGTGAACCCGTTAGGTTTTCTGGAAAAGGAAGAGGCAAAAAAAAGCATTTTCAGTCCTTCGACTTCGATGGAGACCAATATTCACTCGAGGACCCTGTGCTTCTTGTGCCCGAGGTTAAAGATCAAAAGCCGTATGTCGCAATTATCAAGGACATCACACAATCCATAAATGGAAATGGAAGCTTGATGATCACAGGGCAGTGGTTTTACCGTCCAGACGAAGCAGAAAAAAAGGGAGGTGGTAGCTGGCAATCCGTTGACACCAGAGAGCTGTTTTATAGTTTCCACCGAGATGAGGTTCCTGCAGAATCTGTTATGCATAAGTGTGTGGTGCATTTTGTCCCCATACACAAACAACTTCCAAATCGTAAGAAAACTCGTCAACGGTTGGGTGAACTACCTGACATCAAGACTGATGAAGCTGCTGCTGATCAGGAAGATCTTATTAAAAATAGAAGAAACTTTAAGAAGGGTATTTCAGCTATTGATGTTTCAAGGGAGGATGAAACATCTCGAAAGAGTCTCCACTCTCTGAAACCTGGAACACCAGGGAGTTGTCCAGTTATTACCACAGAGCATCAGCGCATATTAGTGAATTTTAATGCGTTAACAGGAGATAGCCATCGTGACAAGGGTTTGGCGATGTTGCTTCAAAATGTTCAATACTTGTTTGACACTGATGAGAGCAAAAAGAAAGAAGACAAATGTGGTGACCGCTCTGATGCTATTAACAATGAAGGCAATGACAAAGTCATTTACCATTGTTTCGCTCTACAGAATTCCAAGTCTTTTGTCTGGCCAGATGTTGCTGTTCCAGCTGTAGTTGCTCTGGAGAAGGCTCTATACCATACATTTTCATCAGATTATCAGAAGTACACCCAAAAGTTACGACAGCTAGTCTTTAATCTCAAGCTGGAATGCATAGCTTGTGGTCATTCCTGGTCTGCCTCTCGTGATGCGGTGTCTGTGCTAACCTTAGATGCATCAGACTCAAAAAGAAATGTAGGCACGGCCCCATGGGCAACTGCGAAATTTGATGTTGGGAAGAAGCTGGCAAGTCCCCGTGAATCTGACAAGACCAATGACATCTTTATGCCGCCTGTTTCCAGGAAAGATGAAACATCGAAGTTTCAAGACGTGCTGATTAGGAGGAGTATTCTATCTTTTGGATATCACTTTTGTTTGGAATTATAG

Protein sequence

>MsG0480023642.01.T01

MKPSDEAEKEEEEEQSSEEEEEEEEEEESPVEDAKPIGEPVRFSGKGRGKKKHFQSFDFDGDQYSLEDPVLLVPEVKDQKPYVAIIKDITQSINGNGSLMITGQWFYRPDEAEKKGGGSWQSVDTRELFYSFHRDEVPAESVMHKCVVHFVPIHKQLPNRKKTRQRLGELPDIKTDEAAADQEDLIKNRRNFKKGISAIDVSREDETSRKSLHSLKPGTPGSCPVITTEHQRILVNFNALTGDSHRDKGLAMLLQNVQYLFDTDESKKKEDKCGDRSDAINNEGNDKVIYHCFALQNSKSFVWPDVAVPAVVALEKALYHTFSSDYQKYTQKLRQLVFNLKLECIACGHSWSASRDAVSVLTLDASDSKRNVGTAPWATAKFDVGKKLASPRESDKTNDIFMPPVSRKDETSKFQDVLIRRSILSFGYHFCLEL*