AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780041662.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780041662.01.T01 MTR_7g115440 99.448 181 1 0 1 181 237 417 1.96e-130 372
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780041662.01.T01 AT5G03970 41.111 180 87 3 2 177 219 383 1.39e-35 128
MsG0780041662.01.T01 AT5G03970 41.111 180 87 3 2 177 231 395 1.66e-35 128

Find 57 sgRNAs with CRISPR-Local

Find 56 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTATGGTCAGAGCAATAATT+TGG 0.246919 7:-93652048 MsG0780041662.01.T01:CDS
GTCGAGGAGCTTCCATTTCT+TGG 0.260659 7:-93651755 MsG0780041662.01.T01:CDS
AGCAGAAGTAGTTAATTATC+AGG 0.265426 7:-93651778 MsG0780041662.01.T01:CDS
ATAATTTGGGTTTGGAGATA+TGG 0.271191 7:-93652034 MsG0780041662.01.T01:CDS
TGCATCTGTGTTTATTAGAC+TGG 0.271482 7:-93651838 MsG0780041662.01.T01:CDS
TGTGCACTGGCTTGCAATTT+GGG 0.279405 7:-93652198 MsG0780041662.01.T01:CDS
TTGTGCACTGGCTTGCAATT+TGG 0.292852 7:-93652199 MsG0780041662.01.T01:CDS
CTATTTATGATCCGCGTCTT+GGG 0.330993 7:-93652166 MsG0780041662.01.T01:CDS
TTCCTTACTTTCTGCCTGCT+TGG 0.355140 7:-93651716 MsG0780041662.01.T01:CDS
GATTGAATTTCAAAGCAATA+TGG 0.367493 7:-93651929 MsG0780041662.01.T01:CDS
TTTGCTAGATTCCCAAGAAA+TGG 0.375579 7:+93651743 None:intergenic
GAGCACGAGGAATCTGTTCT+TGG 0.385966 7:-93652095 MsG0780041662.01.T01:CDS
GCTATTTATGATCCGCGTCT+TGG 0.388579 7:-93652167 MsG0780041662.01.T01:CDS
TAATTTGGGTTTGGAGATAT+GGG 0.390227 7:-93652033 MsG0780041662.01.T01:CDS
TCGAGGAGCTTCCATTTCTT+GGG 0.396105 7:-93651754 MsG0780041662.01.T01:CDS
AACACAGATGCAGAATTCTT+AGG 0.399426 7:+93651849 None:intergenic
TCAGAGCAATAATTTGGGTT+TGG 0.405347 7:-93652042 MsG0780041662.01.T01:CDS
AGCACGAGGAATCTGTTCTT+GGG 0.412726 7:-93652094 MsG0780041662.01.T01:CDS
ACTGTAGGTATGGTGGTTGC+AGG 0.416741 7:-93652224 None:intergenic
TGGGGATAGGAATGTAGCTT+TGG 0.421868 7:-93652147 MsG0780041662.01.T01:CDS
ATTAACTACTTCTGCTGTCT+TGG 0.425023 7:+93651785 None:intergenic
TATGGTCAGAGCAATAATTT+GGG 0.440444 7:-93652047 MsG0780041662.01.T01:CDS
TTGAAATTCAATCTCCACCT+TGG 0.456880 7:+93651939 None:intergenic
GTTCTTGGGGAATCGTCAGA+TGG 0.470864 7:-93652080 MsG0780041662.01.T01:CDS
ATGATCCGCGTCTTGGGGAT+AGG 0.473836 7:-93652160 MsG0780041662.01.T01:CDS
TTAAAATACAAGCTAAAGGT+TGG 0.474334 7:+93651897 None:intergenic
CTGTGTTTATTAGACTGGGC+TGG 0.476248 7:-93651833 MsG0780041662.01.T01:CDS
GATGAAGAGCGTGGCCAAGC+AGG 0.483507 7:+93651702 None:intergenic
GGCCAAGCAGGCAGAAAGTA+AGG 0.488202 7:+93651714 None:intergenic
CTCTTTAAAATACAAGCTAA+AGG 0.488844 7:+93651893 None:intergenic
ATGAGTACAGTTGCAGTAAG+TGG 0.488957 7:+93651980 None:intergenic
GCATCTGTGTTTATTAGACT+GGG 0.489721 7:-93651837 MsG0780041662.01.T01:CDS
GTACTCATTTGAAGTACAGT+TGG 0.495329 7:-93651965 MsG0780041662.01.T01:CDS
TGAAGTACAGTTGGATTCCA+AGG 0.498144 7:-93651956 MsG0780041662.01.T01:CDS
TCAGATGGGCTATTGCAGTA+TGG 0.501832 7:-93652065 MsG0780041662.01.T01:CDS
TGGTTGCAGGAGTTGTGCAC+TGG 0.503026 7:-93652211 MsG0780041662.01.T01:CDS
GGAGATATGGGTTCTTGAGA+AGG 0.509861 7:-93652021 MsG0780041662.01.T01:CDS
TGTAGCTTTGGTTAAACTAC+CGG 0.513326 7:-93652135 MsG0780041662.01.T01:CDS
TAAGTGGATGGGTTAACTGC+TGG 0.537298 7:+93651996 None:intergenic
TTCTTGGGGAATCGTCAGAT+GGG 0.539667 7:-93652079 MsG0780041662.01.T01:CDS
GTAGTTAATTATCAGGGTCG+AGG 0.570044 7:-93651771 MsG0780041662.01.T01:CDS
TATTTATGATCCGCGTCTTG+GGG 0.570373 7:-93652165 MsG0780041662.01.T01:CDS
GTACAGTTGCAGTAAGTGGA+TGG 0.578851 7:+93651984 None:intergenic
TCCTGTGATAACACACCAGC+CGG 0.581421 7:+93652116 None:intergenic
ACCGGCTGGTGTGTTATCAC+AGG 0.587539 7:-93652117 MsG0780041662.01.T01:CDS
TACAGTTGCAGTAAGTGGAT+GGG 0.588665 7:+93651985 None:intergenic
GCAGAAGTAGTTAATTATCA+GGG 0.606553 7:-93651777 MsG0780041662.01.T01:CDS
TTCTTGGGAATCTAGCAAAG+TGG 0.617345 7:-93651739 MsG0780041662.01.T01:CDS
GCACGAGGAATCTGTTCTTG+GGG 0.627375 7:-93652093 MsG0780041662.01.T01:CDS
GCTTTGGTTAAACTACCGGC+TGG 0.627643 7:-93652131 MsG0780041662.01.T01:CDS
GTGCACTGGCTTGCAATTTG+GGG 0.664054 7:-93652197 MsG0780041662.01.T01:CDS
TGTGTTATCACAGGAGCACG+AGG 0.673702 7:-93652108 MsG0780041662.01.T01:CDS
TCTTGGTCTCCAAATCACAC+AGG 0.674843 7:+93651802 None:intergenic
CAAGACACGGATGAAGAGCG+TGG 0.684621 7:+93651693 None:intergenic
AGTACAGTTGGATTCCAAGG+TGG 0.686110 7:-93651953 MsG0780041662.01.T01:CDS
TGTTCCATTCTATCAAGACA+CGG 0.695008 7:+93651680 None:intergenic
ACATTCCTATCCCCAAGACG+CGG 0.709272 7:+93652155 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! GATTGAATTTCAAAGCAATA+TGG - Chr7:93651978-93651997 MsG0780041662.01.T01:CDS 25.0%
! TTAAAATACAAGCTAAAGGT+TGG + Chr7:93652013-93652032 None:intergenic 25.0%
!!! CTCTTTAAAATACAAGCTAA+AGG + Chr7:93652017-93652036 None:intergenic 25.0%
AGCAGAAGTAGTTAATTATC+AGG - Chr7:93652129-93652148 MsG0780041662.01.T01:CDS 30.0%
GCAGAAGTAGTTAATTATCA+GGG - Chr7:93652130-93652149 MsG0780041662.01.T01:CDS 30.0%
TATGGTCAGAGCAATAATTT+GGG - Chr7:93651860-93651879 MsG0780041662.01.T01:CDS 30.0%
! ATAATTTGGGTTTGGAGATA+TGG - Chr7:93651873-93651892 MsG0780041662.01.T01:CDS 30.0%
! TAATTTGGGTTTGGAGATAT+GGG - Chr7:93651874-93651893 MsG0780041662.01.T01:CDS 30.0%
AACACAGATGCAGAATTCTT+AGG + Chr7:93652061-93652080 None:intergenic 35.0%
ATTAACTACTTCTGCTGTCT+TGG + Chr7:93652125-93652144 None:intergenic 35.0%
GCATCTGTGTTTATTAGACT+GGG - Chr7:93652070-93652089 MsG0780041662.01.T01:CDS 35.0%
GTATGGTCAGAGCAATAATT+TGG - Chr7:93651859-93651878 MsG0780041662.01.T01:CDS 35.0%
TGCATCTGTGTTTATTAGAC+TGG - Chr7:93652069-93652088 MsG0780041662.01.T01:CDS 35.0%
TTGAAATTCAATCTCCACCT+TGG + Chr7:93651971-93651990 None:intergenic 35.0%
TTTGCTAGATTCCCAAGAAA+TGG + Chr7:93652167-93652186 None:intergenic 35.0%
! GAACATTTTCCTGTGTGATT+TGG - Chr7:93652096-93652115 MsG0780041662.01.T01:CDS 35.0%
! GTACTCATTTGAAGTACAGT+TGG - Chr7:93651942-93651961 MsG0780041662.01.T01:CDS 35.0%
! TCAGAGCAATAATTTGGGTT+TGG - Chr7:93651865-93651884 MsG0780041662.01.T01:CDS 35.0%
!! TGTAGCTTTGGTTAAACTAC+CGG - Chr7:93651772-93651791 MsG0780041662.01.T01:CDS 35.0%
ATGAGTACAGTTGCAGTAAG+TGG + Chr7:93651930-93651949 None:intergenic 40.0%
CTATTTATGATCCGCGTCTT+GGG - Chr7:93651741-93651760 MsG0780041662.01.T01:CDS 40.0%
GTAGTTAATTATCAGGGTCG+AGG - Chr7:93652136-93652155 MsG0780041662.01.T01:CDS 40.0%
TACAGTTGCAGTAAGTGGAT+GGG + Chr7:93651925-93651944 None:intergenic 40.0%
TATTTATGATCCGCGTCTTG+GGG - Chr7:93651742-93651761 MsG0780041662.01.T01:CDS 40.0%
TGAAGTACAGTTGGATTCCA+AGG - Chr7:93651951-93651970 MsG0780041662.01.T01:CDS 40.0%
TTCTTGGGAATCTAGCAAAG+TGG - Chr7:93652168-93652187 MsG0780041662.01.T01:CDS 40.0%
AGCACGAGGAATCTGTTCTT+GGG - Chr7:93651813-93651832 MsG0780041662.01.T01:CDS 45.0%
AGTACAGTTGGATTCCAAGG+TGG - Chr7:93651954-93651973 MsG0780041662.01.T01:CDS 45.0%
CTGTGTTTATTAGACTGGGC+TGG - Chr7:93652074-93652093 MsG0780041662.01.T01:CDS 45.0%
GCTATTTATGATCCGCGTCT+TGG - Chr7:93651740-93651759 MsG0780041662.01.T01:CDS 45.0%
GGAGATATGGGTTCTTGAGA+AGG - Chr7:93651886-93651905 MsG0780041662.01.T01:CDS 45.0%
GTACAGTTGCAGTAAGTGGA+TGG + Chr7:93651926-93651945 None:intergenic 45.0%
TCGAGGAGCTTCCATTTCTT+GGG - Chr7:93652153-93652172 MsG0780041662.01.T01:CDS 45.0%
TCTTGGTCTCCAAATCACAC+AGG + Chr7:93652108-93652127 None:intergenic 45.0%
TGGGGATAGGAATGTAGCTT+TGG - Chr7:93651760-93651779 MsG0780041662.01.T01:CDS 45.0%
TTCCTTACTTTCTGCCTGCT+TGG - Chr7:93652191-93652210 MsG0780041662.01.T01:CDS 45.0%
TTCTTGGGGAATCGTCAGAT+GGG - Chr7:93651828-93651847 MsG0780041662.01.T01:CDS 45.0%
!! TAAGTGGATGGGTTAACTGC+TGG + Chr7:93651914-93651933 None:intergenic 45.0%
!! TCAGATGGGCTATTGCAGTA+TGG - Chr7:93651842-93651861 MsG0780041662.01.T01:CDS 45.0%
!! TGTGCACTGGCTTGCAATTT+GGG - Chr7:93651709-93651728 MsG0780041662.01.T01:CDS 45.0%
!! TTGTGCACTGGCTTGCAATT+TGG - Chr7:93651708-93651727 MsG0780041662.01.T01:CDS 45.0%
ACATTCCTATCCCCAAGACG+CGG + Chr7:93651755-93651774 None:intergenic 50.0%
GAGCACGAGGAATCTGTTCT+TGG - Chr7:93651812-93651831 MsG0780041662.01.T01:CDS 50.0%
GCACGAGGAATCTGTTCTTG+GGG - Chr7:93651814-93651833 MsG0780041662.01.T01:CDS 50.0%
GCTTTGGTTAAACTACCGGC+TGG - Chr7:93651776-93651795 MsG0780041662.01.T01:CDS 50.0%
GTCGAGGAGCTTCCATTTCT+TGG - Chr7:93652152-93652171 MsG0780041662.01.T01:CDS 50.0%
GTTCTTGGGGAATCGTCAGA+TGG - Chr7:93651827-93651846 MsG0780041662.01.T01:CDS 50.0%
TCCTGTGATAACACACCAGC+CGG + Chr7:93651794-93651813 None:intergenic 50.0%
! TGTGTTATCACAGGAGCACG+AGG - Chr7:93651799-93651818 MsG0780041662.01.T01:CDS 50.0%
!! GTGCACTGGCTTGCAATTTG+GGG - Chr7:93651710-93651729 MsG0780041662.01.T01:CDS 50.0%
ACCGGCTGGTGTGTTATCAC+AGG - Chr7:93651790-93651809 MsG0780041662.01.T01:CDS 55.0%
ATGATCCGCGTCTTGGGGAT+AGG - Chr7:93651747-93651766 MsG0780041662.01.T01:CDS 55.0%
CAAGACACGGATGAAGAGCG+TGG + Chr7:93652217-93652236 None:intergenic 55.0%
GGCCAAGCAGGCAGAAAGTA+AGG + Chr7:93652196-93652215 None:intergenic 55.0%
TGGTTGCAGGAGTTGTGCAC+TGG - Chr7:93651696-93651715 MsG0780041662.01.T01:CDS 55.0%
! GATGAAGAGCGTGGCCAAGC+AGG + Chr7:93652208-93652227 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
Chr7 gene 93651692 93652237 93651692 ID=MsG0780041662.01;Name=MsG0780041662.01
Chr7 mRNA 93651692 93652237 93651692 ID=MsG0780041662.01.T01;Parent=MsG0780041662.01;Name=MsG0780041662.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|181
Chr7 exon 93651692 93652237 93651692 ID=MsG0780041662.01.T01:exon:19691;Parent=MsG0780041662.01.T01
Chr7 CDS 93651692 93652237 93651692 ID=MsG0780041662.01.T01:cds;Parent=MsG0780041662.01.T01
Gene Sequence

>MsG0780041662.01.T01

ATGGTGGTTGCAGGAGTTGTGCACTGGCTTGCAATTTGGGGAAAACTTGCTATTTATGATCCGCGTCTTGGGGATAGGAATGTAGCTTTGGTTAAACTACCGGCTGGTGTGTTATCACAGGAGCACGAGGAATCTGTTCTTGGGGAATCGTCAGATGGGCTATTGCAGTATGGTCAGAGCAATAATTTGGGTTTGGAGATATGGGTTCTTGAGAAGGAGCCAGCAGTTAACCCATCCACTTACTGCAACTGTACTCATTTGAAGTACAGTTGGATTCCAAGGTGGAGATTGAATTTCAAAGCAATATGGAAGCAGATACCAACCTTTAGCTTGTATTTTAAAGAGACTCAGATACTATCATTTCTTCCTAAGAATTCTGCATCTGTGTTTATTAGACTGGGCTGGAACATTTTCCTGTGTGATTTGGAGACCAAGACAGCAGAAGTAGTTAATTATCAGGGTCGAGGAGCTTCCATTTCTTGGGAATCTAGCAAAGTGGTTCCTTACTTTCTGCCTGCTTGGCCACGCTCTTCATCCGTGTCTTGA

Protein sequence

>MsG0780041662.01.T01

MVVAGVVHWLAIWGKLAIYDPRLGDRNVALVKLPAGVLSQEHEESVLGESSDGLLQYGQSNNLGLEIWVLEKEPAVNPSTYCNCTHLKYSWIPRWRLNFKAIWKQIPTFSLYFKETQILSFLPKNSASVFIRLGWNIFLCDLETKTAEVVNYQGRGASISWESSKVVPYFLPAWPRSSSVS*