AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480023777.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480023777.01.T01 MTR_8g102030 99.550 222 1 0 1 222 1 222 2.41e-160 442
MsG0480023777.01.T01 MTR_6g090515 81.364 220 39 2 5 222 1 220 1.11e-105 304
MsG0480023777.01.T01 MTR_8g102020 67.411 224 52 5 1 222 1 205 1.98e-72 219
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480023777.01.T01 AT2G19830 77.027 222 38 7 5 222 1 213 6.46e-98 284
MsG0480023777.01.T01 AT4G29160 77.828 221 44 4 5 222 1 219 7.99e-93 271
MsG0480023777.01.T01 AT4G29160 77.828 221 44 4 5 222 1 219 7.99e-93 271
MsG0480023777.01.T01 AT2G19830 79.293 198 31 5 28 222 4 194 6.73e-90 263
MsG0480023777.01.T01 AT4G29160 80.000 180 33 3 46 222 13 192 9.80e-83 244

Find 73 sgRNAs with CRISPR-Local

Find 248 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACGAACCCTGGTGAGAAATT+AGG 0.197502 4:+89663358 None:intergenic
TCACAAGAGCCAAGAATAAA+AGG 0.227877 4:-89662742 MsG0480023777.01.T01:CDS
ACTTGGAATGCTAGAGAAAA+AGG 0.296376 4:-89662816 MsG0480023777.01.T01:CDS
GGAGAGCGTGCTTCTTAAAA+AGG 0.301195 4:-89662795 MsG0480023777.01.T01:CDS
GGGACATGCACAGAGGCTTC+TGG 0.308869 4:+89659909 None:intergenic
GGATTCTGTTGCAGGGAGAC+TGG 0.311823 4:-89663283 MsG0480023777.01.T01:intron
CGAACCCTGGTGAGAAATTA+GGG 0.313711 4:+89663359 None:intergenic
TGAGAACATGAAACAGATTC+AGG 0.382236 4:-89660187 MsG0480023777.01.T01:CDS
AAATTAGGGTTTGGAATAAG+AGG 0.383107 4:+89663373 None:intergenic
TGTGGGCCGCGCTTAGTAGA+AGG 0.389765 4:-89663540 MsG0480023777.01.T01:five_prime_UTR
GCCCACTCCTGAGGAAGAAT+TGG 0.390463 4:-89659853 MsG0480023777.01.T01:CDS
GCTTCTGGAGTTGTAATTGC+AGG 0.391518 4:+89659924 None:intergenic
GGACGAGTAGGCCGCCTAGC+TGG 0.394730 4:+89659888 None:intergenic
TACGGGGCATACATAAGAAT+TGG 0.406525 4:-89659508 MsG0480023777.01.T01:three_prime_UTR
TACTGTTTCTGTGGTTGCCT+TGG 0.415099 4:+89660645 None:intergenic
CCAGTTGCGTATTCATGATC+AGG 0.417336 4:-89661920 MsG0480023777.01.T01:intron
AGCTAGGCGGCCTACTCGTC+CGG 0.432309 4:-89659886 MsG0480023777.01.T01:CDS
GCTGAGATGGCGCTTTGAGC+AGG 0.432581 4:-89659819 MsG0480023777.01.T01:intron
CCAAACCCTAATTTCTCACC+AGG 0.441030 4:-89663364 MsG0480023777.01.T01:five_prime_UTR
GAATTATTGTCCGCTCCAAT+TGG 0.445452 4:-89660165 MsG0480023777.01.T01:CDS
TTCTAAAGTTGATAGAGTAT+TGG 0.445759 4:+89663182 None:intergenic
CACAAGAGCCAAGAATAAAA+GGG 0.447210 4:-89662741 MsG0480023777.01.T01:CDS
AAATCAGCAGCTGCACCAAT+TGG 0.448641 4:+89660150 None:intergenic
CCTGGTGAGAAATTAGGGTT+TGG 0.450323 4:+89663364 None:intergenic
GGCAGCTTTGCAAGCTGAGA+TGG 0.454546 4:-89659832 MsG0480023777.01.T01:CDS
ACAGTAGCCGCATTGAGAAC+TGG 0.461367 4:-89660628 MsG0480023777.01.T01:CDS
TGGTTAGGTCAGAATTTAGC+AGG 0.466064 4:-89659549 MsG0480023777.01.T01:intron
AGAGCTAGAGGGTGCTGAGT+TGG 0.470621 4:-89659964 MsG0480023777.01.T01:CDS
GCCAATTCTTCCTCAGGAGT+GGG 0.471301 4:+89659852 None:intergenic
AAAGCTGCCAATTCTTCCTC+AGG 0.474769 4:+89659846 None:intergenic
GGCTGCAGCTGAAGTTGAAA+GGG 0.480403 4:-89662774 MsG0480023777.01.T01:CDS
GATTCTGTTGCAGGGAGACT+GGG 0.481277 4:-89663282 MsG0480023777.01.T01:intron
CGGCGATACAATGTTTGAAA+AGG 0.488220 4:-89661984 MsG0480023777.01.T01:CDS
CCTGATCATGAATACGCAAC+TGG 0.506321 4:+89661920 None:intergenic
AGGCTGCAGCTGAAGTTGAA+AGG 0.506350 4:-89662775 MsG0480023777.01.T01:CDS
GACGAGTAGGCCGCCTAGCT+GGG 0.521270 4:+89659889 None:intergenic
TACAATGTTTGAAAAGGAAG+AGG 0.525703 4:-89661978 MsG0480023777.01.T01:CDS
ATTGATGATGAGAATGATAC+GGG 0.531507 4:-89659525 MsG0480023777.01.T01:three_prime_UTR
CATTGATGATGAGAATGATA+CGG 0.539696 4:-89659526 MsG0480023777.01.T01:three_prime_UTR
CCTCTGTGCATGTCCCAGCT+AGG 0.540963 4:-89659902 MsG0480023777.01.T01:CDS
AAGATAAAAGCGACTGTGAG+AGG 0.541206 4:+89659462 None:intergenic
GCGTGCAAGTGCAAATGCGA+TGG 0.547146 4:+89663455 None:intergenic
GGCAGAATTCTCCATGTCAC+TGG 0.548290 4:+89659483 None:intergenic
CTGTTCGTTAATCTCATCCA+TGG 0.550173 4:+89660212 None:intergenic
TGCCAATTCTTCCTCAGGAG+TGG 0.551831 4:+89659851 None:intergenic
TTTGTATGCACCGGACGAGT+AGG 0.560328 4:+89659876 None:intergenic
GCTTGAATTATGTAGACACT+TGG 0.569639 4:-89662833 MsG0480023777.01.T01:intron
TGTGTTACAGATGATAATGC+TGG 0.571958 4:-89660674 MsG0480023777.01.T01:intron
CAATGCGGCTACTGTTTCTG+TGG 0.580774 4:+89660636 None:intergenic
AGCAGAACTTGAAGAGCTAG+AGG 0.585998 4:-89659976 MsG0480023777.01.T01:CDS
GATAATGCTGGAAGGTGCCA+AGG 0.586807 4:-89660662 MsG0480023777.01.T01:CDS
GAACAGCAAGTAGAGCAGCT+TGG 0.594879 4:-89661949 MsG0480023777.01.T01:CDS
GGAGTGGGCTTTGTATGCAC+CGG 0.596065 4:+89659867 None:intergenic
TTTGTGAAATTATTGAACAG+AGG 0.605498 4:-89659441 MsG0480023777.01.T01:three_prime_UTR
GCCGCGCTTAGTAGAAGGGA+GGG 0.610430 4:-89663535 MsG0480023777.01.T01:five_prime_UTR
AAGAATTGGAACCAGTGACA+TGG 0.617295 4:-89659494 MsG0480023777.01.T01:three_prime_UTR
CGTGCAAGTGCAAATGCGAT+GGG 0.619756 4:+89663456 None:intergenic
TAATGATGTTGATGCGACCA+TGG 0.625933 4:-89660229 MsG0480023777.01.T01:CDS
AAACGAAATAGAGAAAGCGA+AGG 0.628174 4:+89663498 None:intergenic
GGCCGCGCTTAGTAGAAGGG+AGG 0.635506 4:-89663536 MsG0480023777.01.T01:five_prime_UTR
ACAAGAGCCAAGAATAAAAG+GGG 0.640430 4:-89662740 MsG0480023777.01.T01:intron
AGCAGCTGCACCAATTGGAG+CGG 0.641867 4:+89660155 None:intergenic
GCAGAACTTGAAGAGCTAGA+GGG 0.644080 4:-89659975 MsG0480023777.01.T01:CDS
GCATACAAAGCCCACTCCTG+AGG 0.653225 4:-89659862 MsG0480023777.01.T01:CDS
CAAACCCTAATTTCTCACCA+GGG 0.653246 4:-89663363 MsG0480023777.01.T01:intron
ATCTACCAAGGTGACAACAA+AGG 0.654532 4:+89659380 None:intergenic
TCCCTCCCTTCTACTAAGCG+CGG 0.657112 4:+89663534 None:intergenic
AGCCGCATTGAGAACTGGAG+CGG 0.658271 4:-89660623 MsG0480023777.01.T01:CDS
CTGTGCATGTCCCAGCTAGG+CGG 0.671027 4:-89659899 MsG0480023777.01.T01:CDS
CCTAGCTGGGACATGCACAG+AGG 0.679296 4:+89659902 None:intergenic
TTACAGATGATAATGCTGGA+AGG 0.735879 4:-89660670 MsG0480023777.01.T01:intron
AGCCGCTCCAGTTCTCAATG+CGG 0.737111 4:+89660621 None:intergenic
TTGATGATGAGAATGATACG+GGG 0.784907 4:-89659524 MsG0480023777.01.T01:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATATATATATATATATAT+TGG - Chr4:89662391-89662410 MsG0480023777.01.T01:intron 0.0%
!! ATTATTATTATATTATTTAA+AGG - Chr4:89661950-89661969 MsG0480023777.01.T01:CDS 0.0%
!! AAACATGTATAAATAAATAA+AGG - Chr4:89661712-89661731 MsG0480023777.01.T01:intron 10.0%
!! GTAAACAAATATTATATTTA+CGG - Chr4:89661678-89661697 MsG0480023777.01.T01:intron 10.0%
!! TAAACAAATATTATATTTAC+GGG - Chr4:89661679-89661698 MsG0480023777.01.T01:intron 10.0%
!! TATAATTATATATCATTACT+AGG + Chr4:89661653-89661672 None:intergenic 10.0%
!!! TATTATTTATAAGTTTGTTA+AGG + Chr4:89661333-89661352 None:intergenic 10.0%
!! AAAACTTATCTATAGAAATT+TGG + Chr4:89660724-89660743 None:intergenic 15.0%
!! AATTCATTCAATAAAATGTT+CGG - Chr4:89663539-89663558 MsG0480023777.01.T01:five_prime_UTR 15.0%
!! ATTATGAAATTGTGATAAAA+AGG + Chr4:89659558-89659577 None:intergenic 15.0%
!! GTAATAAATCAATTGAATTA+TGG + Chr4:89661757-89661776 None:intergenic 15.0%
!! TAAATAAATAAAACTCCTAA+TGG - Chr4:89659724-89659743 MsG0480023777.01.T01:intron 15.0%
!! TACTTATTATGTGAAATATT+CGG - Chr4:89662831-89662850 MsG0480023777.01.T01:intron 15.0%
!!! ACTTTTTTAAAGAATATAAG+TGG - Chr4:89660174-89660193 MsG0480023777.01.T01:CDS 15.0%
!!! CATATTGATTCTAAATAAAT+TGG - Chr4:89661913-89661932 MsG0480023777.01.T01:intron 15.0%
!!! CATTAATATGTTTTTATGAT+TGG - Chr4:89663118-89663137 MsG0480023777.01.T01:intron 15.0%
!!! CTTTTTTAAAGAATATAAGT+GGG - Chr4:89660175-89660194 MsG0480023777.01.T01:CDS 15.0%
!!! GTTTATATTATATTGCTATT+CGG - Chr4:89659825-89659844 MsG0480023777.01.T01:CDS 15.0%
!!! TTGCAATATTATATTTTTTC+AGG - Chr4:89662613-89662632 MsG0480023777.01.T01:intron 15.0%
!!! TTTGTTTCCATATATTATTT+TGG - Chr4:89660217-89660236 MsG0480023777.01.T01:CDS 15.0%
!! AAGTTCTCCAAAATAATATA+TGG + Chr4:89660227-89660246 None:intergenic 20.0%
!! AATAATATATGGAAACAAAC+AGG + Chr4:89660216-89660235 None:intergenic 20.0%
!! ATAATATATGGAAACAAACA+GGG + Chr4:89660215-89660234 None:intergenic 20.0%
!! ATATATATATTGGTGTGTTA+AGG - Chr4:89662441-89662460 MsG0480023777.01.T01:intron 20.0%
!! ATCTACGTTAATATACAAAA+TGG + Chr4:89661136-89661155 None:intergenic 20.0%
!! ATTCATTCAATAAAATGTTC+GGG - Chr4:89663540-89663559 MsG0480023777.01.T01:five_prime_UTR 20.0%
!! CAATTTCATAATTCTCATAT+TGG - Chr4:89659566-89659585 MsG0480023777.01.T01:intron 20.0%
!! TAAAAAAACAGTGCAAAAAA+TGG + Chr4:89662046-89662065 None:intergenic 20.0%
!! TTGTATATTAACGTAGATAT+AGG - Chr4:89661138-89661157 MsG0480023777.01.T01:intron 20.0%
!! TTTATAATATTCTTCTGATG+TGG - Chr4:89662511-89662530 MsG0480023777.01.T01:intron 20.0%
!!! AACTTTAGAAAAATTAAGTG+AGG - Chr4:89659701-89659720 MsG0480023777.01.T01:intron 20.0%
!!! ACATTCATTATTTTCAAAAG+TGG - Chr4:89661022-89661041 MsG0480023777.01.T01:intron 20.0%
!!! ATATGTTTTTATGATTGGTA+TGG - Chr4:89663123-89663142 MsG0480023777.01.T01:intron 20.0%
!!! ATTCTTTAAAAAAGTATCCA+TGG + Chr4:89660169-89660188 None:intergenic 20.0%
!!! ATTTTTTGCACTGTTTTTTT+AGG - Chr4:89662045-89662064 MsG0480023777.01.T01:intron 20.0%
!!! CAATTGATTTATTACATTAC+TGG - Chr4:89661762-89661781 MsG0480023777.01.T01:intron 20.0%
!!! TAATAATGGATTTCTAAAGA+GGG + Chr4:89660793-89660812 None:intergenic 20.0%
!!! TATTTTGAGCTTATGAATAT+AGG - Chr4:89661179-89661198 MsG0480023777.01.T01:intron 20.0%
!!! TTCTTTAAAAAAGTATCCAT+GGG + Chr4:89660168-89660187 None:intergenic 20.0%
!!! TTTTTTGCACTGTTTTTTTA+GGG - Chr4:89662046-89662065 MsG0480023777.01.T01:intron 20.0%
!!! TTTTTTTGTAGAAAATTGAC+AGG + Chr4:89661475-89661494 None:intergenic 20.0%
! AAAATAATGAATGTAACTGC+TGG + Chr4:89661016-89661035 None:intergenic 25.0%
! AAAATGTGAACTGATAGAAA+AGG - Chr4:89660615-89660634 MsG0480023777.01.T01:CDS 25.0%
! AAAGTTTCTTCATAGTTGAT+TGG - Chr4:89660497-89660516 MsG0480023777.01.T01:intron 25.0%
! AAGAAAAATTGAGTTCCATT+AGG + Chr4:89659742-89659761 None:intergenic 25.0%
! AAGGGAAAAATATCCATTTA+AGG + Chr4:89661608-89661627 None:intergenic 25.0%
! AGAAACTATAAAAATGCCTT+TGG + Chr4:89661504-89661523 None:intergenic 25.0%
! AGGGAAAAATATCCATTTAA+GGG + Chr4:89661607-89661626 None:intergenic 25.0%
! AGTAGTATCCTTATAACTAT+AGG + Chr4:89659929-89659948 None:intergenic 25.0%
! ATTAATGTATAGCAGTTGAA+TGG - Chr4:89663148-89663167 MsG0480023777.01.T01:intron 25.0%
! CAATTAAGCCTATAGTTATA+AGG - Chr4:89659918-89659937 MsG0480023777.01.T01:CDS 25.0%
! CATTAAATCGCTAAAAAGAA+AGG - Chr4:89661087-89661106 MsG0480023777.01.T01:intron 25.0%
! CTAAAATAAGCTAATCCAAA+CGG - Chr4:89661395-89661414 MsG0480023777.01.T01:intron 25.0%
! GAAACTATAAAAATGCCTTT+GGG + Chr4:89661503-89661522 None:intergenic 25.0%
! TACGTTAATATACAAAATGG+TGG + Chr4:89661133-89661152 None:intergenic 25.0%
! TAGATTACTAGAATGAAAGT+GGG - Chr4:89660644-89660663 MsG0480023777.01.T01:CDS 25.0%
! TTTAAAGAATATAAGTGGGA+CGG - Chr4:89660179-89660198 MsG0480023777.01.T01:CDS 25.0%
! TTTGTGAAATTATTGAACAG+AGG - Chr4:89663429-89663448 MsG0480023777.01.T01:five_prime_UTR 25.0%
!! CTTTAGAAATCCATTATTAG+TGG - Chr4:89660794-89660813 MsG0480023777.01.T01:intron 25.0%
!! TCAGTTCACATTTTATAAAG+TGG + Chr4:89660609-89660628 None:intergenic 25.0%
!! TCTTTCTTATCTTTAGGTTA+CGG - Chr4:89662572-89662591 MsG0480023777.01.T01:intron 25.0%
!! TTCTATAGATAAGTTTTACC+TGG - Chr4:89660727-89660746 MsG0480023777.01.T01:intron 25.0%
!! TTGTCTTTTATATCTTATGC+TGG - Chr4:89662168-89662187 MsG0480023777.01.T01:intron 25.0%
!!! AATAATGGATTTCTAAAGAG+GGG + Chr4:89660792-89660811 None:intergenic 25.0%
!!! ATTTTTAGCATAAAATGTCC+AGG + Chr4:89660748-89660767 None:intergenic 25.0%
!!! CTAATAATGGATTTCTAAAG+AGG + Chr4:89660794-89660813 None:intergenic 25.0%
!!! CTTTCTTTTTAGCGATTTAA+TGG + Chr4:89661089-89661108 None:intergenic 25.0%
!!! GAATCTTTTTAGCGATTTAA+TGG + Chr4:89661053-89661072 None:intergenic 25.0%
!!! TTCTAAAGTTGATAGAGTAT+TGG + Chr4:89659691-89659710 None:intergenic 25.0%
!!! TTGTATTTAGTTTCTAGCAA+TGG - Chr4:89659969-89659988 MsG0480023777.01.T01:CDS 25.0%
!!! TTTTTATTTTCACTCTGCTA+TGG - Chr4:89662767-89662786 MsG0480023777.01.T01:CDS 25.0%
AAGTGTAGAATAGAATGATC+AGG + Chr4:89660699-89660718 None:intergenic 30.0%
AATTGATAAGCACGTTATTG+TGG - Chr4:89659868-89659887 MsG0480023777.01.T01:CDS 30.0%
ACTTATTTGAGTCTATCTAC+TGG + Chr4:89660333-89660352 None:intergenic 30.0%
CTAGATTACTAGAATGAAAG+TGG - Chr4:89660643-89660662 MsG0480023777.01.T01:CDS 30.0%
CTATAGGCTTAATTGAGATA+TGG + Chr4:89659913-89659932 None:intergenic 30.0%
TAACAAGAACTGCTTAAAAC+AGG + Chr4:89663280-89663299 None:intergenic 30.0%
TAACGCTCTTTCTTATCTTT+AGG - Chr4:89662566-89662585 MsG0480023777.01.T01:intron 30.0%
TACAAAAAAAATCTGCCCAA+AGG - Chr4:89661485-89661504 MsG0480023777.01.T01:intron 30.0%
TACAATGTTTGAAAAGGAAG+AGG - Chr4:89660892-89660911 MsG0480023777.01.T01:intron 30.0%
TCGCTAAAAAGATTCAAAAC+TGG - Chr4:89661058-89661077 MsG0480023777.01.T01:intron 30.0%
TGAATTTCTATCTATTGCAG+CGG - Chr4:89660866-89660885 MsG0480023777.01.T01:intron 30.0%
TGCGTAAAAAAAATCTACCA+AGG + Chr4:89663505-89663524 None:intergenic 30.0%
TTAAAGAATATAAGTGGGAC+GGG - Chr4:89660180-89660199 MsG0480023777.01.T01:CDS 30.0%
! AAATTAGGGTTTGGAATAAG+AGG + Chr4:89659500-89659519 None:intergenic 30.0%
! ATTTGACAATTTTCTCCTGA+TGG - Chr4:89661546-89661565 MsG0480023777.01.T01:intron 30.0%
! TCCATGTTTTCAAAGCTTTT+TGG - Chr4:89659642-89659661 MsG0480023777.01.T01:intron 30.0%
! TTCTTCATAGTTGATTGGTT+TGG - Chr4:89660502-89660521 MsG0480023777.01.T01:intron 30.0%
! TTTTGTAGAAAATTGACAGG+TGG + Chr4:89661472-89661491 None:intergenic 30.0%
!! AAGATTTTTGTGGTAGTGTT+TGG + Chr4:89660309-89660328 None:intergenic 30.0%
!! ACCAAAAAGCTTTGAAAACA+TGG + Chr4:89659646-89659665 None:intergenic 30.0%
!! AGATTTTTGTGGTAGTGTTT+GGG + Chr4:89660308-89660327 None:intergenic 30.0%
!! ATTGATGATGAGAATGATAC+GGG - Chr4:89663345-89663364 MsG0480023777.01.T01:intron 30.0%
!! CATTGATGATGAGAATGATA+CGG - Chr4:89663344-89663363 MsG0480023777.01.T01:intron 30.0%
!! CTACTTTTCTTGCAAAACTT+AGG - Chr4:89661522-89661541 MsG0480023777.01.T01:intron 30.0%
!! CTTTTTAGCGATTTAATGGT+TGG + Chr4:89661049-89661068 None:intergenic 30.0%
!! GAAAGAGCGTTAAACAAAAA+AGG + Chr4:89662558-89662577 None:intergenic 30.0%
!! GCTTTTTGGTAAAACTAAAC+AGG - Chr4:89659656-89659675 MsG0480023777.01.T01:intron 30.0%
!!! AAAAAAGTATCCATGGGTTT+TGG + Chr4:89660162-89660181 None:intergenic 30.0%
AAACGAAATAGAGAAAGCGA+AGG + Chr4:89659375-89659394 None:intergenic 35.0%
AAATCCATTATTAGTGGAGC+AGG - Chr4:89660800-89660819 MsG0480023777.01.T01:intron 35.0%
AAGGAATGATGAGAAGAAGA+AGG + Chr4:89659539-89659558 None:intergenic 35.0%
ACAAGAGCCAAGAATAAAAG+GGG - Chr4:89660130-89660149 MsG0480023777.01.T01:intron 35.0%
ACATGGACATTTCCCTTAAA+TGG - Chr4:89661592-89661611 MsG0480023777.01.T01:intron 35.0%
ACTTGGAATGCTAGAGAAAA+AGG - Chr4:89660054-89660073 MsG0480023777.01.T01:intron 35.0%
AGCAATGGTTTACAGTTTGA+GGG - Chr4:89659984-89660003 MsG0480023777.01.T01:CDS 35.0%
ATATAGAATGAGAGCAGAAG+GGG - Chr4:89661837-89661856 MsG0480023777.01.T01:intron 35.0%
ATGAATTGCTTGAAGCTGTT+TGG - Chr4:89663237-89663256 MsG0480023777.01.T01:CDS 35.0%
ATGACCTATAGAAGCAGTTA+AGG - Chr4:89660537-89660556 MsG0480023777.01.T01:intron 35.0%
CAAAATAAGCTCCATCCAAA+CGG + Chr4:89661166-89661185 None:intergenic 35.0%
CACAAGAGCCAAGAATAAAA+GGG - Chr4:89660129-89660148 MsG0480023777.01.T01:intron 35.0%
CATATAGAATGAGAGCAGAA+GGG - Chr4:89661836-89661855 MsG0480023777.01.T01:intron 35.0%
CATTGCTCAAATGATTGAAC+TGG + Chr4:89661977-89661996 None:intergenic 35.0%
CCTTCATTGAAGAGAAACTT+GGG - Chr4:89660449-89660468 MsG0480023777.01.T01:intron 35.0%
GACTTGCACTTGTGTTAATT+TGG - Chr4:89660389-89660408 MsG0480023777.01.T01:intron 35.0%
GCTTGAATTATGTAGACACT+TGG - Chr4:89660037-89660056 MsG0480023777.01.T01:intron 35.0%
TAACACAAGTGCAAGTCATA+AGG + Chr4:89660386-89660405 None:intergenic 35.0%
TAGAAAATTGACAGGTGGTT+GGG + Chr4:89661467-89661486 None:intergenic 35.0%
TAGCAATGGTTTACAGTTTG+AGG - Chr4:89659983-89660002 MsG0480023777.01.T01:CDS 35.0%
TATAGCAGTTGAATGGTTTG+TGG - Chr4:89663155-89663174 MsG0480023777.01.T01:intron 35.0%
TATATTGGTGTGTTAAGGCT+TGG - Chr4:89662446-89662465 MsG0480023777.01.T01:intron 35.0%
TCACAAGAGCCAAGAATAAA+AGG - Chr4:89660128-89660147 MsG0480023777.01.T01:intron 35.0%
TCATATAGAATGAGAGCAGA+AGG - Chr4:89661835-89661854 MsG0480023777.01.T01:intron 35.0%
TCATATTGGATTCTGTTGCA+GGG - Chr4:89659580-89659599 MsG0480023777.01.T01:intron 35.0%
TGAGAACATGAAACAGATTC+AGG - Chr4:89662683-89662702 MsG0480023777.01.T01:intron 35.0%
TGATATATTGCTTCTCGCTT+TGG - Chr4:89663301-89663320 MsG0480023777.01.T01:intron 35.0%
TGCGATTTCATGCAAAAACA+TGG - Chr4:89661575-89661594 MsG0480023777.01.T01:intron 35.0%
TGTATTGTATGCGAGTCTAT+GGG + Chr4:89661422-89661441 None:intergenic 35.0%
TTAAGAACTGATCCTCCAAT+CGG + Chr4:89662081-89662100 None:intergenic 35.0%
TTATCAGAACAAGACTGAAC+TGG + Chr4:89662123-89662142 None:intergenic 35.0%
TTATGAATATAGGCTCCGTT+TGG - Chr4:89661189-89661208 MsG0480023777.01.T01:intron 35.0%
! AAACTTTACCCTCTTTTCCA+CGG + Chr4:89660484-89660503 None:intergenic 35.0%
! AGTGCAAAAAATGGAAGTAC+TGG + Chr4:89662037-89662056 None:intergenic 35.0%
! ATCTACTGGCAAGATTTTTG+TGG + Chr4:89660319-89660338 None:intergenic 35.0%
! TCTGAATTTGTTTGCCATTC+AGG - Chr4:89662861-89662880 MsG0480023777.01.T01:intron 35.0%
! TGTGTTACAGATGATAATGC+TGG - Chr4:89662196-89662215 MsG0480023777.01.T01:intron 35.0%
! TTACAGATGATAATGCTGGA+AGG - Chr4:89662200-89662219 MsG0480023777.01.T01:intron 35.0%
!! TGAACTATGACTTGTTCATG+CGG - Chr4:89661995-89662014 MsG0480023777.01.T01:intron 35.0%
!! TTATAAAGTGGTGTCGTAGT+AGG + Chr4:89660597-89660616 None:intergenic 35.0%
!! TTGATGATGAGAATGATACG+GGG - Chr4:89663346-89663365 MsG0480023777.01.T01:intron 35.0%
AAGAATTGGAACCAGTGACA+TGG - Chr4:89663376-89663395 MsG0480023777.01.T01:five_prime_UTR 40.0%
AAGATAAAAGCGACTGTGAG+AGG + Chr4:89663411-89663430 None:intergenic 40.0%
ACAAGTGCAAGTCATAAGGA+GGG + Chr4:89660382-89660401 None:intergenic 40.0%
AGAACTGCTTAAAACAGGAC+AGG + Chr4:89663275-89663294 None:intergenic 40.0%
AGTTTCTCTTCAATGAAGGC+TGG + Chr4:89660448-89660467 None:intergenic 40.0%
ATCTACCAAGGTGACAACAA+AGG + Chr4:89663493-89663512 None:intergenic 40.0%
CAAACCCTAATTTCTCACCA+GGG - Chr4:89659507-89659526 MsG0480023777.01.T01:three_prime_UTR 40.0%
CATAAGGAGGGATACACTAT+GGG + Chr4:89660370-89660389 None:intergenic 40.0%
CCCAAGTTTCTCTTCAATGA+AGG + Chr4:89660452-89660471 None:intergenic 40.0%
CCTATAGAAGCAGTTAAGGA+CGG - Chr4:89660541-89660560 MsG0480023777.01.T01:intron 40.0%
CGGCGATACAATGTTTGAAA+AGG - Chr4:89660886-89660905 MsG0480023777.01.T01:intron 40.0%
CTCATATTGGATTCTGTTGC+AGG - Chr4:89659579-89659598 MsG0480023777.01.T01:intron 40.0%
CTGTTCGTTAATCTCATCCA+TGG + Chr4:89662661-89662680 None:intergenic 40.0%
GAAATCGCATAAAAGCCATC+AGG + Chr4:89661564-89661583 None:intergenic 40.0%
GAATTATTGTCCGCTCCAAT+TGG - Chr4:89662705-89662724 MsG0480023777.01.T01:intron 40.0%
GCCTTCATTGAAGAGAAACT+TGG - Chr4:89660448-89660467 MsG0480023777.01.T01:intron 40.0%
GTAGAAAATTGACAGGTGGT+TGG + Chr4:89661468-89661487 None:intergenic 40.0%
GTGTATTGTATGCGAGTCTA+TGG + Chr4:89661423-89661442 None:intergenic 40.0%
TAATGATGTTGATGCGACCA+TGG - Chr4:89662641-89662660 MsG0480023777.01.T01:intron 40.0%
TACGGGGCATACATAAGAAT+TGG - Chr4:89663362-89663381 MsG0480023777.01.T01:intron 40.0%
TATTGCTTCTCGCTTTGGTT+AGG - Chr4:89663306-89663325 MsG0480023777.01.T01:intron 40.0%
TCATAAGGAGGGATACACTA+TGG + Chr4:89660371-89660390 None:intergenic 40.0%
TGAACCTGCTCCACTAATAA+TGG + Chr4:89660807-89660826 None:intergenic 40.0%
TGGTTAGGTCAGAATTTAGC+AGG - Chr4:89663321-89663340 MsG0480023777.01.T01:intron 40.0%
! GTACGTACCCCTTTTATTCT+TGG + Chr4:89660140-89660159 None:intergenic 40.0%
! TAAGGCTTGGTTGTTGTTGT+TGG - Chr4:89662459-89662478 MsG0480023777.01.T01:intron 40.0%
! TGCTTCAAGTTCATCCTGAA+TGG + Chr4:89662878-89662897 None:intergenic 40.0%
!! GAACTATGACTTGTTCATGC+GGG - Chr4:89661996-89662015 MsG0480023777.01.T01:intron 40.0%
!! TTTTGCCTTTGTTGTCACCT+TGG - Chr4:89663485-89663504 MsG0480023777.01.T01:five_prime_UTR 40.0%
AAAGCTGCCAATTCTTCCTC+AGG + Chr4:89663027-89663046 None:intergenic 45.0%
AAATCAGCAGCTGCACCAAT+TGG + Chr4:89662723-89662742 None:intergenic 45.0%
ACGAACCCTGGTGAGAAATT+AGG + Chr4:89659515-89659534 None:intergenic 45.0%
ACGTAGATATAGGCTCCGTT+TGG - Chr4:89661148-89661167 MsG0480023777.01.T01:intron 45.0%
AGAATGAAAGTGGGAGTGAC+TGG - Chr4:89660653-89660672 MsG0480023777.01.T01:CDS 45.0%
AGATATAGGCTCCGTTTGGA+TGG - Chr4:89661152-89661171 MsG0480023777.01.T01:intron 45.0%
AGCAGAACTTGAAGAGCTAG+AGG - Chr4:89662894-89662913 MsG0480023777.01.T01:intron 45.0%
ATAGTGTCGTAGCCGAGAAA+AGG + Chr4:89663079-89663098 None:intergenic 45.0%
CACAAGTGCAAGTCATAAGG+AGG + Chr4:89660383-89660402 None:intergenic 45.0%
CAGGTAGTTCGGTAGGTTTA+GGG - Chr4:89660569-89660588 MsG0480023777.01.T01:intron 45.0%
CCAAACCCTAATTTCTCACC+AGG - Chr4:89659506-89659525 MsG0480023777.01.T01:three_prime_UTR 45.0%
CCAGTTGCGTATTCATGATC+AGG - Chr4:89660950-89660969 MsG0480023777.01.T01:intron 45.0%
CCGTCCTTAACTGCTTCTAT+AGG + Chr4:89660544-89660563 None:intergenic 45.0%
CCTGATCATGAATACGCAAC+TGG + Chr4:89660953-89660972 None:intergenic 45.0%
CCTGGTGAGAAATTAGGGTT+TGG + Chr4:89659509-89659528 None:intergenic 45.0%
CGAACCCTGGTGAGAAATTA+GGG + Chr4:89659514-89659533 None:intergenic 45.0%
GAATATAGGCTCCGTTTGGA+TGG - Chr4:89661193-89661212 MsG0480023777.01.T01:intron 45.0%
GAATGAAAGTGGGAGTGACT+GGG - Chr4:89660654-89660673 MsG0480023777.01.T01:CDS 45.0%
GATACACTATGGGCTCTGTT+TGG + Chr4:89660360-89660379 None:intergenic 45.0%
GCAGAACTTGAAGAGCTAGA+GGG - Chr4:89662895-89662914 MsG0480023777.01.T01:intron 45.0%
TAGTGTCGTAGCCGAGAAAA+GGG + Chr4:89663078-89663097 None:intergenic 45.0%
TCAGGTAGTTCGGTAGGTTT+AGG - Chr4:89660568-89660587 MsG0480023777.01.T01:intron 45.0%
! GGAGAGCGTGCTTCTTAAAA+AGG - Chr4:89660075-89660094 MsG0480023777.01.T01:intron 45.0%
! TTTACCCTCTTTTCCACGGT+TGG + Chr4:89660480-89660499 None:intergenic 45.0%
! TTTCCCTTGAGCTTTTGCGT+TGG - Chr4:89661620-89661639 MsG0480023777.01.T01:intron 45.0%
! TTTGAGCAGGTCCCTTTTCT+CGG - Chr4:89663064-89663083 MsG0480023777.01.T01:intron 45.0%
!! GCTTCTGGAGTTGTAATTGC+AGG + Chr4:89662949-89662968 None:intergenic 45.0%
!! TACTGTTTCTGTGGTTGCCT+TGG + Chr4:89662228-89662247 None:intergenic 45.0%
!! TTAGGGTTGAGTTGACCGAT+TGG - Chr4:89662063-89662082 MsG0480023777.01.T01:intron 45.0%
ACAGTAGCCGCATTGAGAAC+TGG - Chr4:89662242-89662261 MsG0480023777.01.T01:intron 50.0%
AGCACCAACCGTGGAAAAGA+GGG - Chr4:89660473-89660492 MsG0480023777.01.T01:intron 50.0%
AGCACCAACGCAAAAGCTCA+AGG + Chr4:89661627-89661646 None:intergenic 50.0%
AGGCTGCAGCTGAAGTTGAA+AGG - Chr4:89660095-89660114 MsG0480023777.01.T01:intron 50.0%
CGTGCAAGTGCAAATGCGAT+GGG + Chr4:89659417-89659436 None:intergenic 50.0%
GAACAGCAAGTAGAGCAGCT+TGG - Chr4:89660921-89660940 MsG0480023777.01.T01:intron 50.0%
GAAGAAGAAGGAACGAACCC+TGG + Chr4:89659527-89659546 None:intergenic 50.0%
GATTCTGTTGCAGGGAGACT+GGG - Chr4:89659588-89659607 MsG0480023777.01.T01:intron 50.0%
GCACCAACGCAAAAGCTCAA+GGG + Chr4:89661626-89661645 None:intergenic 50.0%
GCCAATTCTTCCTCAGGAGT+GGG + Chr4:89663021-89663040 None:intergenic 50.0%
GGCAGAATTCTCCATGTCAC+TGG + Chr4:89663390-89663409 None:intergenic 50.0%
GGCTGCAGCTGAAGTTGAAA+GGG - Chr4:89660096-89660115 MsG0480023777.01.T01:intron 50.0%
TAGCACCAACCGTGGAAAAG+AGG - Chr4:89660472-89660491 MsG0480023777.01.T01:intron 50.0%
TGCCAATTCTTCCTCAGGAG+TGG + Chr4:89663022-89663041 None:intergenic 50.0%
TTTGTATGCACCGGACGAGT+AGG + Chr4:89662997-89663016 None:intergenic 50.0%
! ACTTGTTCATGCGGGTTGAC+TGG - Chr4:89662004-89662023 MsG0480023777.01.T01:intron 50.0%
!! CAATGCGGCTACTGTTTCTG+TGG + Chr4:89662237-89662256 None:intergenic 50.0%
!! GATAATGCTGGAAGGTGCCA+AGG - Chr4:89662208-89662227 MsG0480023777.01.T01:intron 50.0%
!!! TGCAGCTGCTGATTTTGACG+AGG - Chr4:89662728-89662747 MsG0480023777.01.T01:intron 50.0%
AACTTGGGTAGCACCAACCG+TGG - Chr4:89660464-89660483 MsG0480023777.01.T01:intron 55.0%
AGCAGCTGCACCAATTGGAG+CGG + Chr4:89662718-89662737 None:intergenic 55.0%
AGCCGCATTGAGAACTGGAG+CGG - Chr4:89662247-89662266 MsG0480023777.01.T01:intron 55.0%
AGCCGCTCCAGTTCTCAATG+CGG + Chr4:89662252-89662271 None:intergenic 55.0%
AGGTGGTTGGGCTTCTGATC+GGG + Chr4:89661455-89661474 None:intergenic 55.0%
CAGGTGGTTGGGCTTCTGAT+CGG + Chr4:89661456-89661475 None:intergenic 55.0%
GCAGTTAAGGACGGCAGATC+AGG - Chr4:89660550-89660569 MsG0480023777.01.T01:intron 55.0%
GCATACAAAGCCCACTCCTG+AGG - Chr4:89663008-89663027 MsG0480023777.01.T01:intron 55.0%
GCCCACTCCTGAGGAAGAAT+TGG - Chr4:89663017-89663036 MsG0480023777.01.T01:intron 55.0%
GCGTGCAAGTGCAAATGCGA+TGG + Chr4:89659418-89659437 None:intergenic 55.0%
GGACGGCAGATCAGGTAGTT+CGG - Chr4:89660558-89660577 MsG0480023777.01.T01:intron 55.0%
GGATTCTGTTGCAGGGAGAC+TGG - Chr4:89659587-89659606 MsG0480023777.01.T01:intron 55.0%
GGCAGATCAGGTAGTTCGGT+AGG - Chr4:89660562-89660581 MsG0480023777.01.T01:intron 55.0%
GGCAGCTTTGCAAGCTGAGA+TGG - Chr4:89663038-89663057 MsG0480023777.01.T01:intron 55.0%
GGGGTACGTACCAAAACCCA+TGG - Chr4:89660149-89660168 MsG0480023777.01.T01:CDS 55.0%
TCCCTCCCTTCTACTAAGCG+CGG + Chr4:89659339-89659358 None:intergenic 55.0%
TGAGAGCAGAAGGGGAAGAC+AGG - Chr4:89661845-89661864 MsG0480023777.01.T01:intron 55.0%
TGCGAGTCTATGGGTCCGTT+TGG + Chr4:89661413-89661432 None:intergenic 55.0%
! GGAGTGGGCTTTGTATGCAC+CGG + Chr4:89663006-89663025 None:intergenic 55.0%
!! AGAGCTAGAGGGTGCTGAGT+TGG - Chr4:89662906-89662925 MsG0480023777.01.T01:intron 55.0%
!! GGGTTGAGTTGACCGATTGG+AGG - Chr4:89662066-89662085 MsG0480023777.01.T01:intron 55.0%
CCTAGCTGGGACATGCACAG+AGG + Chr4:89662971-89662990 None:intergenic 60.0%
CCTCTGTGCATGTCCCAGCT+AGG - Chr4:89662968-89662987 MsG0480023777.01.T01:intron 60.0%
CTGTGCATGTCCCAGCTAGG+CGG - Chr4:89662971-89662990 MsG0480023777.01.T01:intron 60.0%
GCCGCGCTTAGTAGAAGGGA+GGG - Chr4:89659335-89659354 MsG0480023777.01.T01:three_prime_UTR 60.0%
GGGACATGCACAGAGGCTTC+TGG + Chr4:89662964-89662983 None:intergenic 60.0%
! GCTGAGATGGCGCTTTGAGC+AGG - Chr4:89663051-89663070 MsG0480023777.01.T01:intron 60.0%
AGCTAGGCGGCCTACTCGTC+CGG - Chr4:89662984-89663003 MsG0480023777.01.T01:intron 65.0%
GACGAGTAGGCCGCCTAGCT+GGG + Chr4:89662984-89663003 None:intergenic 65.0%
GGCCGCGCTTAGTAGAAGGG+AGG - Chr4:89659334-89659353 MsG0480023777.01.T01:three_prime_UTR 65.0%
GGACGAGTAGGCCGCCTAGC+TGG + Chr4:89662985-89663004 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
Chr4 gene 89659333 89663559 89659333 ID=MsG0480023777.01;Name=MsG0480023777.01
Chr4 mRNA 89659333 89663559 89659333 ID=MsG0480023777.01.T01;Parent=MsG0480023777.01;Name=MsG0480023777.01.T01;_AED=0.40;_eAED=0.40;_QI=228|1|1|1|0.57|0.37|8|236|222
Chr4 exon 89659333 89659564 89659333 ID=MsG0480023777.01.T01:exon:33681;Parent=MsG0480023777.01.T01
Chr4 exon 89659820 89660009 89659820 ID=MsG0480023777.01.T01:exon:33680;Parent=MsG0480023777.01.T01
Chr4 exon 89660143 89660257 89660143 ID=MsG0480023777.01.T01:exon:33679;Parent=MsG0480023777.01.T01
Chr4 exon 89660595 89660686 89660595 ID=MsG0480023777.01.T01:exon:33678;Parent=MsG0480023777.01.T01
Chr4 exon 89661921 89662006 89661921 ID=MsG0480023777.01.T01:exon:33677;Parent=MsG0480023777.01.T01
Chr4 exon 89662741 89662840 89662741 ID=MsG0480023777.01.T01:exon:33676;Parent=MsG0480023777.01.T01
Chr4 exon 89663170 89663291 89663170 ID=MsG0480023777.01.T01:exon:33675;Parent=MsG0480023777.01.T01
Chr4 exon 89663364 89663559 89663364 ID=MsG0480023777.01.T01:exon:33674;Parent=MsG0480023777.01.T01
Chr4 five_prime_UTR 89663364 89663559 89663364 ID=MsG0480023777.01.T01:five_prime_utr;Parent=MsG0480023777.01.T01
Chr4 five_prime_UTR 89663260 89663291 89663260 ID=MsG0480023777.01.T01:five_prime_utr;Parent=MsG0480023777.01.T01
Chr4 CDS 89663170 89663259 89663170 ID=MsG0480023777.01.T01:cds;Parent=MsG0480023777.01.T01
Chr4 CDS 89662741 89662840 89662741 ID=MsG0480023777.01.T01:cds;Parent=MsG0480023777.01.T01
Chr4 CDS 89661921 89662006 89661921 ID=MsG0480023777.01.T01:cds;Parent=MsG0480023777.01.T01
Chr4 CDS 89660595 89660686 89660595 ID=MsG0480023777.01.T01:cds;Parent=MsG0480023777.01.T01
Chr4 CDS 89660143 89660257 89660143 ID=MsG0480023777.01.T01:cds;Parent=MsG0480023777.01.T01
Chr4 CDS 89659824 89660009 89659824 ID=MsG0480023777.01.T01:cds;Parent=MsG0480023777.01.T01
Chr4 three_prime_UTR 89659820 89659823 89659820 ID=MsG0480023777.01.T01:three_prime_utr;Parent=MsG0480023777.01.T01
Chr4 three_prime_UTR 89659333 89659564 89659333 ID=MsG0480023777.01.T01:three_prime_utr;Parent=MsG0480023777.01.T01
Gene Sequence

>MsG0480023777.01.T01

ATGAAATCATCCATGTTTTCAAAGCTTTTTGGTAAAACTAAACAGGATCAACAAGCCAATACTCTATCAACTTTAGAAAAATTAAGTGAGACACTTGGAATGCTAGAGAAAAAGGAGAGCGTGCTTCTTAAAAAGGCTGCAGCTGAAGTTGAAAGGGCTAAAGAATTCACAAGAGCCAAGAATAAAAGGGCGGCGATACAATGTTTGAAAAGGAAGAGGCTATATGAACAGCAAGTAGAGCAGCTTGGAAACTTCCAGTTGCGTATTCATGATCAGATGATAATGCTGGAAGGTGCCAAGGCAACCACAGAAACAGTAGCCGCATTGAGAACTGGAGCGGCTGCTATGAAGACTATGCAACAAGAAACGAATATTAATGATGTTGATGCGACCATGGATGAGATTAACGAACAGACTGAGAACATGAAACAGATTCAGGAATTATTGTCCGCTCCAATTGGTGCAGCTGCTGATTTTGACGAGGATGAACTTGAAGCAGAACTTGAAGAGCTAGAGGGTGCTGAGTTGGAAGAACAGCTTCTTCAGCCTGCAATTACAACTCCAGAAGCCTCTGTGCATGTCCCAGCTAGGCGGCCTACTCGTCCGGTGCATACAAAGCCCACTCCTGAGGAAGAATTGGCAGCTTTGCAAGCTGAGATGGCGCTTTGA
Protein sequence

>MsG0480023777.01.T01

MKSSMFSKLFGKTKQDQQANTLSTLEKLSETLGMLEKKESVLLKKAAAEVERAKEFTRAKNKRAAIQCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVAALRTGAAAMKTMQQETNINDVDATMDEINEQTENMKQIQELLSAPIGAAADFDEDELEAELEELEGAELEEQLLQPAITTPEASVHVPARRPTRPVHTKPTPEEELAALQAEMAL*