AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480023731.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480023731.01.T01 MTR_8g100065 95.745 188 5 2 1 187 1 186 2.22e-131 366
MsG0480023731.01.T01 MTR_8g100065 97.093 172 5 0 16 187 37 208 1.11e-125 352
MsG0480023731.01.T01 MTR_6g077990 76.966 178 39 2 10 186 23 199 5.99e-95 275
MsG0480023731.01.T01 MTR_6g077990 79.290 169 33 2 19 186 31 198 1.23e-93 271
MsG0480023731.01.T01 MTR_6g077990 78.846 156 32 1 31 186 2 156 2.67e-87 253
MsG0480023731.01.T01 MTR_8g100065 93.893 131 5 2 1 130 1 129 1.86e-84 245
MsG0480023731.01.T01 MTR_8g100065 95.652 115 5 0 16 130 37 151 6.43e-79 232
MsG0480023731.01.T01 MTR_1g033750 53.261 184 85 1 3 186 41 223 9.74e-67 204
MsG0480023731.01.T01 MTR_1g114640 60.000 160 63 1 24 183 60 218 1.71e-63 196
MsG0480023731.01.T01 MTR_6g077990 79.130 115 22 2 19 132 31 144 1.17e-58 181
MsG0480023731.01.T01 MTR_1g114690 52.071 169 80 1 18 186 51 218 2.84e-56 177
MsG0480023731.01.T01 MTR_1g114690 51.497 167 80 1 18 184 51 216 7.20e-56 176
MsG0480023731.01.T01 MTR_1g114750 46.409 181 96 1 6 186 42 221 1.85e-55 175
MsG0480023731.01.T01 MTR_1g115130 52.667 150 70 1 37 186 46 194 1.11e-50 162
MsG0480023731.01.T01 MTR_1g114690 43.781 201 78 2 18 184 51 250 5.52e-49 159
MsG0480023731.01.T01 MTR_7g083570 41.975 162 93 1 23 184 115 275 4.16e-42 143
MsG0480023731.01.T01 MTR_3g100750 36.538 156 97 1 28 183 102 255 1.81e-39 135
MsG0480023731.01.T01 MTR_1g114640 60.185 108 42 1 24 131 60 166 3.63e-39 132
MsG0480023731.01.T01 MTR_8g069110 37.888 161 98 1 23 183 109 267 7.14e-39 134
MsG0480023731.01.T01 MTR_1g114680 60.000 110 43 1 75 184 53 161 9.58e-38 129
MsG0480023731.01.T01 MTR_2g076350 35.802 162 102 2 23 183 65 225 1.91e-31 114
MsG0480023731.01.T01 MTR_1g114690 50.442 113 55 1 18 130 51 162 6.79e-31 112
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480023731.01.T01 AT5G23370 54.878 164 73 1 24 187 55 217 1.84e-59 185
MsG0480023731.01.T01 AT5G08350 54.878 164 73 1 24 187 54 216 1.18e-58 183
MsG0480023731.01.T01 AT5G23350 54.658 161 72 1 24 184 55 214 8.40e-57 178
MsG0480023731.01.T01 AT5G23360 52.174 161 76 1 24 184 47 206 8.28e-55 173
MsG0480023731.01.T01 AT5G13200 39.506 162 97 1 23 184 101 261 1.78e-40 138
MsG0480023731.01.T01 AT1G28200 37.267 161 99 1 23 183 96 254 1.25e-39 135
MsG0480023731.01.T01 AT1G28200 37.267 161 99 1 23 183 96 254 1.25e-39 135
MsG0480023731.01.T01 AT4G01600 37.654 162 101 0 22 183 24 185 9.89e-39 131
MsG0480023731.01.T01 AT2G22475 35.625 160 101 1 24 183 134 291 1.28e-38 134
MsG0480023731.01.T01 AT4G01600 37.888 161 100 0 23 183 64 224 2.78e-38 131
MsG0480023731.01.T01 AT4G01600 39.456 147 89 0 37 183 73 219 1.11e-35 125
MsG0480023731.01.T01 AT5G13200 41.584 101 59 0 23 123 101 201 4.73e-24 94.4
MsG0480023731.01.T01 AT2G22475 33.333 117 76 1 24 140 134 248 8.79e-21 86.7
MsG0480023731.01.T01 AT4G40100 31.111 135 63 1 49 183 112 216 1.34e-19 83.2

Find 45 sgRNAs with CRISPR-Local

Find 81 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAATGGAAATTTCTTATTTC+TGG 0.091487 4:+89124231 None:intergenic
CTCACGATGATTCTGAATTT+TGG 0.150158 4:-89123032 MsG0480023731.01.T01:CDS
ATCTATCTTCTTCTTCTTGT+AGG 0.202658 4:-89124336 MsG0480023731.01.T01:five_prime_UTR
TTTAGCAGTTGCAGTTTCTT+TGG 0.245252 4:+89124051 None:intergenic
CTTCTTCTTGTAGGTTTCAT+TGG 0.252901 4:-89124327 MsG0480023731.01.T01:five_prime_UTR
AGAAACTGCAACTGCTAAAA+TGG 0.264589 4:-89124047 MsG0480023731.01.T01:CDS
CAATGTGCAGTAGGGCTAAA+TGG 0.279415 4:+89122948 None:intergenic
AGTGTGAAGTTCTATGTAAA+TGG 0.297354 4:-89122866 MsG0480023731.01.T01:three_prime_UTR
GATGCAGAAGAGAAGGTTAT+TGG 0.316549 4:+89123415 None:intergenic
TCTGAAGGGTAAGTTGAGTT+TGG 0.345403 4:-89123611 MsG0480023731.01.T01:CDS
AATCCTACATGCAAAACTTC+TGG 0.347012 4:+89123997 None:intergenic
TTCAGAGTCTCTGATAGTTT+AGG 0.396250 4:+89123628 None:intergenic
CTGAAGGGTAAGTTGAGTTT+GGG 0.410973 4:-89123610 MsG0480023731.01.T01:CDS
ACAACAGCTGGTCCTATTGC+TGG 0.416930 4:-89123484 MsG0480023731.01.T01:CDS
TGAAGGGCAGAATGTGAATC+AGG 0.430005 4:-89123082 MsG0480023731.01.T01:CDS
ACCTTCTCTTCTGCATCAAC+GGG 0.443671 4:-89123409 MsG0480023731.01.T01:CDS
ATGAAAAGAACTCCAGCAAT+AGG 0.446868 4:+89123472 None:intergenic
TGTTATATCTTGCAAACAGC+TGG 0.447335 4:+89124300 None:intergenic
ATGAATTCTTCTACTTCTAC+AGG 0.454002 4:-89124204 MsG0480023731.01.T01:intron
TAATAATTACGTACCTTGTA+CGG 0.463291 4:+89123370 None:intergenic
AACCTTCTCTTCTGCATCAA+CGG 0.469610 4:-89123410 MsG0480023731.01.T01:CDS
AAAATCAAACAATGTGCAGT+AGG 0.473818 4:+89122939 None:intergenic
GGGCTAAATGGACATTGAAA+TGG 0.476559 4:+89122960 None:intergenic
GTGTGAAGTTCTATGTAAAT+GGG 0.492938 4:-89122865 MsG0480023731.01.T01:three_prime_UTR
TTTATGTTTGAGATGTACAT+AGG 0.493478 4:-89122899 MsG0480023731.01.T01:three_prime_UTR
GTAAGTTGAGTTTGGGTGCA+AGG 0.498584 4:-89123603 MsG0480023731.01.T01:CDS
AAGATAAAAGAAGTCAATGA+AGG 0.502485 4:-89123099 MsG0480023731.01.T01:CDS
TAGAAGAATTCATCAGTGAA+TGG 0.512531 4:+89124214 None:intergenic
AGAAGGAGAGAAAGTGTTGA+AGG 0.521050 4:-89123527 MsG0480023731.01.T01:CDS
ATAATACAAGAAGGTGGGAG+AGG 0.525733 4:-89123580 MsG0480023731.01.T01:CDS
TGTTACTTATACACAACAGC+TGG 0.553623 4:-89123496 MsG0480023731.01.T01:CDS
AAATTCATTGCAGTAAAACT+AGG 0.557356 4:-89123652 MsG0480023731.01.T01:intron
AAGAAGAAGATAGATAGAGA+TGG 0.566356 4:+89124344 None:intergenic
AAATCAAACAATGTGCAGTA+GGG 0.570196 4:+89122940 None:intergenic
TCCCGTTGATGCAGAAGAGA+AGG 0.582493 4:+89123408 None:intergenic
AGCACAAGAAAGCAACAACC+AGG 0.598769 4:-89124024 MsG0480023731.01.T01:CDS
GCAAGGATAATACAAGAAGG+TGG 0.599451 4:-89123586 MsG0480023731.01.T01:CDS
TAATACAAGAAGGTGGGAGA+GGG 0.599558 4:-89123579 MsG0480023731.01.T01:CDS
TAAACTATCAGAGACTCTGA+AGG 0.601920 4:-89123626 MsG0480023731.01.T01:CDS
GCATGTAGGATTCATGAACA+TGG 0.604739 4:-89123986 MsG0480023731.01.T01:intron
CAAGGATAATACAAGAAGGT+GGG 0.615352 4:-89123585 MsG0480023731.01.T01:CDS
AGATAAAAGAAGTCAATGAA+GGG 0.635091 4:-89123098 MsG0480023731.01.T01:CDS
GACGCGCAAGATACCGTACA+AGG 0.636816 4:-89123383 MsG0480023731.01.T01:intron
AAACTATCAGAGACTCTGAA+GGG 0.658037 4:-89123625 MsG0480023731.01.T01:CDS
GGTGCAAGGATAATACAAGA+AGG 0.670404 4:-89123589 MsG0480023731.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATAATCAATATTTTTTTTAG+GGG - Chr4:89123433-89123452 MsG0480023731.01.T01:CDS 10.0%
!!! AACATTTTCAAGAATATTTT+TGG - Chr4:89123627-89123646 MsG0480023731.01.T01:CDS 15.0%
!!! ATAATTTGGTTTATTGATTT+AGG - Chr4:89123905-89123924 MsG0480023731.01.T01:intron 15.0%
!!! TTTTGAATGTAATTAATTGA+AGG + Chr4:89123033-89123052 None:intergenic 15.0%
!! AAGAGAGAAAGAAAAAAAAA+AGG + Chr4:89123474-89123493 None:intergenic 20.0%
!!! ATTTGTTGTATTAACTGTTT+CGG - Chr4:89124007-89124026 MsG0480023731.01.T01:CDS 20.0%
!!! TTGATTTGCACTTTATAATT+TGG - Chr4:89123891-89123910 MsG0480023731.01.T01:intron 20.0%
! AAATTCATTGCAGTAAAACT+AGG - Chr4:89123531-89123550 MsG0480023731.01.T01:CDS 25.0%
! AAGATAAAAGAAGTCAATGA+AGG - Chr4:89124084-89124103 MsG0480023731.01.T01:intron 25.0%
! AATAGAATTGTAGTTGTTTG+TGG - Chr4:89124358-89124377 MsG0480023731.01.T01:five_prime_UTR 25.0%
! AGATAAAAGAAGTCAATGAA+GGG - Chr4:89124085-89124104 MsG0480023731.01.T01:intron 25.0%
! AGTTTGATATGTTAAAGTTG+AGG + Chr4:89123972-89123991 None:intergenic 25.0%
! ATACTACTAATATATTGACG+TGG - Chr4:89123089-89123108 MsG0480023731.01.T01:CDS 25.0%
! ATGTAATTAATTGAAGGTTG+TGG + Chr4:89123027-89123046 None:intergenic 25.0%
! TAATAATTACGTACCTTGTA+CGG + Chr4:89123816-89123835 None:intergenic 25.0%
! TATTTACCTCACAAATTAAC+TGG - Chr4:89123237-89123256 MsG0480023731.01.T01:intron 25.0%
!! ACTACAAAATGCAACTTTTT+GGG + Chr4:89123742-89123761 None:intergenic 25.0%
!! GAATGGAAATTTCTTATTTC+TGG + Chr4:89122955-89122974 None:intergenic 25.0%
!! TAGATTAGTTATGTGTTAGT+TGG - Chr4:89123359-89123378 MsG0480023731.01.T01:intron 25.0%
!! TTTATGTTTGAGATGTACAT+AGG - Chr4:89124284-89124303 MsG0480023731.01.T01:five_prime_UTR 25.0%
!!! AATATTTTTGGTATGCAAGA+AGG - Chr4:89123639-89123658 MsG0480023731.01.T01:CDS 25.0%
!!! GATTCTGAATTTTGGTTTAT+GGG - Chr4:89124159-89124178 MsG0480023731.01.T01:intron 25.0%
!!! TGATTCTGAATTTTGGTTTA+TGG - Chr4:89124158-89124177 MsG0480023731.01.T01:intron 25.0%
AAAATCAAACAATGTGCAGT+AGG + Chr4:89124247-89124266 None:intergenic 30.0%
AAATCAAACAATGTGCAGTA+GGG + Chr4:89124246-89124265 None:intergenic 30.0%
AAGAAGAAGATAGATAGAGA+TGG + Chr4:89122842-89122861 None:intergenic 30.0%
AGTGTGAAGTTCTATGTAAA+TGG - Chr4:89124317-89124336 MsG0480023731.01.T01:five_prime_UTR 30.0%
ATAGTAATAGAGCTTATGAG+TGG - Chr4:89123866-89123885 MsG0480023731.01.T01:intron 30.0%
ATCTATCTTCTTCTTCTTGT+AGG - Chr4:89122847-89122866 MsG0480023731.01.T01:three_prime_UTR 30.0%
ATGAATTCTTCTACTTCTAC+AGG - Chr4:89122979-89122998 MsG0480023731.01.T01:CDS 30.0%
CTCATAAGCTCTATTACTAT+TGG + Chr4:89123866-89123885 None:intergenic 30.0%
GTGTGAAGTTCTATGTAAAT+GGG - Chr4:89124318-89124337 MsG0480023731.01.T01:five_prime_UTR 30.0%
TAGAAGAATTCATCAGTGAA+TGG + Chr4:89122972-89122991 None:intergenic 30.0%
TATTAACTGTTTCGGTATCA+AGG - Chr4:89124015-89124034 MsG0480023731.01.T01:CDS 30.0%
TCGGTATCAAGGATTTAATT+AGG - Chr4:89124026-89124045 MsG0480023731.01.T01:CDS 30.0%
TGATATGTTAAAGTTGAGGT+CGG + Chr4:89123968-89123987 None:intergenic 30.0%
TGTGAGGTAAATATTTAGAG+AGG + Chr4:89123230-89123249 None:intergenic 30.0%
! ACTTCTTTTATCTTCCCAAT+AGG + Chr4:89124078-89124097 None:intergenic 30.0%
! CACTACAAAATGCAACTTTT+TGG + Chr4:89123743-89123762 None:intergenic 30.0%
AAACTATCAGAGACTCTGAA+GGG - Chr4:89123558-89123577 MsG0480023731.01.T01:CDS 35.0%
AATCCTACATGCAAAACTTC+TGG + Chr4:89123189-89123208 None:intergenic 35.0%
AGAAACTGCAACTGCTAAAA+TGG - Chr4:89123136-89123155 MsG0480023731.01.T01:intron 35.0%
ATGAAAAGAACTCCAGCAAT+AGG + Chr4:89123714-89123733 None:intergenic 35.0%
CAAGGATAATACAAGAAGGT+GGG - Chr4:89123598-89123617 MsG0480023731.01.T01:CDS 35.0%
CTTCTTCTTGTAGGTTTCAT+TGG - Chr4:89122856-89122875 MsG0480023731.01.T01:three_prime_UTR 35.0%
TAAACTATCAGAGACTCTGA+AGG - Chr4:89123557-89123576 MsG0480023731.01.T01:CDS 35.0%
TGTTATATCTTGCAAACAGC+TGG + Chr4:89122886-89122905 None:intergenic 35.0%
TTCAGAGTCTCTGATAGTTT+AGG + Chr4:89123558-89123577 None:intergenic 35.0%
TTTAGCAGTTGCAGTTTCTT+TGG + Chr4:89123135-89123154 None:intergenic 35.0%
! AAACCAGAAGTTTTGCATGT+AGG - Chr4:89123183-89123202 MsG0480023731.01.T01:intron 35.0%
! CTCACGATGATTCTGAATTT+TGG - Chr4:89124151-89124170 MsG0480023731.01.T01:intron 35.0%
! TACGCTATGAGAAAGCTTTT+AGG - Chr4:89124187-89124206 MsG0480023731.01.T01:intron 35.0%
! TATGTGTTAGTTGGTCTACT+TGG - Chr4:89123368-89123387 MsG0480023731.01.T01:intron 35.0%
! TGTTACTTATACACAACAGC+TGG - Chr4:89123687-89123706 MsG0480023731.01.T01:intron 35.0%
! TTAGGTGCATTTTACTATGC+AGG - Chr4:89124044-89124063 MsG0480023731.01.T01:CDS 35.0%
!! ACAAAATGCAACTTTTTGGG+TGG + Chr4:89123739-89123758 None:intergenic 35.0%
!! ATGCAGGTTTTGATACCTAT+TGG - Chr4:89124060-89124079 MsG0480023731.01.T01:intron 35.0%
!! TGCAGGTTTTGATACCTATT+GGG - Chr4:89124061-89124080 MsG0480023731.01.T01:intron 35.0%
AACCTTCTCTTCTGCATCAA+CGG - Chr4:89123773-89123792 MsG0480023731.01.T01:intron 40.0%
ATAATACAAGAAGGTGGGAG+AGG - Chr4:89123603-89123622 MsG0480023731.01.T01:CDS 40.0%
GATGCAGAAGAGAAGGTTAT+TGG + Chr4:89123771-89123790 None:intergenic 40.0%
GCAAGGATAATACAAGAAGG+TGG - Chr4:89123597-89123616 MsG0480023731.01.T01:CDS 40.0%
GCATGTAGGATTCATGAACA+TGG - Chr4:89123197-89123216 MsG0480023731.01.T01:intron 40.0%
GGGCTAAATGGACATTGAAA+TGG + Chr4:89124226-89124245 None:intergenic 40.0%
GGTGCAAGGATAATACAAGA+AGG - Chr4:89123594-89123613 MsG0480023731.01.T01:CDS 40.0%
TAATACAAGAAGGTGGGAGA+GGG - Chr4:89123604-89123623 MsG0480023731.01.T01:CDS 40.0%
! CTGAAGGGTAAGTTGAGTTT+GGG - Chr4:89123573-89123592 MsG0480023731.01.T01:CDS 40.0%
! TCTGAAGGGTAAGTTGAGTT+TGG - Chr4:89123572-89123591 MsG0480023731.01.T01:CDS 40.0%
!! AGAAGGAGAGAAAGTGTTGA+AGG - Chr4:89123656-89123675 MsG0480023731.01.T01:intron 40.0%
!! CAGTAGCCAGTTAATTTGTG+AGG + Chr4:89123246-89123265 None:intergenic 40.0%
!!! TGCAAAACTTCTGGTTTTCC+TGG + Chr4:89123180-89123199 None:intergenic 40.0%
ACCTTCTCTTCTGCATCAAC+GGG - Chr4:89123774-89123793 MsG0480023731.01.T01:intron 45.0%
AGCACAAGAAAGCAACAACC+AGG - Chr4:89123159-89123178 MsG0480023731.01.T01:intron 45.0%
CAATGTGCAGTAGGGCTAAA+TGG + Chr4:89124238-89124257 None:intergenic 45.0%
TGAAGGGCAGAATGTGAATC+AGG - Chr4:89124101-89124120 MsG0480023731.01.T01:intron 45.0%
! GTAAGTTGAGTTTGGGTGCA+AGG - Chr4:89123580-89123599 MsG0480023731.01.T01:CDS 45.0%
!!! AATAATCAATATTTTTTTTA+GGG - Chr4:89123432-89123451 MsG0480023731.01.T01:CDS 5.0%
!!! TAATAATCAATATTTTTTTT+AGG - Chr4:89123431-89123450 MsG0480023731.01.T01:CDS 5.0%
ACAACAGCTGGTCCTATTGC+TGG - Chr4:89123699-89123718 MsG0480023731.01.T01:intron 50.0%
TCCCGTTGATGCAGAAGAGA+AGG + Chr4:89123778-89123797 None:intergenic 50.0%
GACGCGCAAGATACCGTACA+AGG - Chr4:89123800-89123819 MsG0480023731.01.T01:intron 55.0%
Chromosome Type Strat End Strand Name
Chr4 gene 89122827 89124378 89122827 ID=MsG0480023731.01;Name=MsG0480023731.01
Chr4 mRNA 89122827 89124378 89122827 ID=MsG0480023731.01.T01;Parent=MsG0480023731.01;Name=MsG0480023731.01.T01;_AED=0.45;_eAED=0.45;_QI=152|1|1|1|0.66|0.5|4|136|187
Chr4 exon 89122827 89123139 89122827 ID=MsG0480023731.01.T01:exon:12454;Parent=MsG0480023731.01.T01
Chr4 exon 89123384 89123661 89123384 ID=MsG0480023731.01.T01:exon:12453;Parent=MsG0480023731.01.T01
Chr4 exon 89123987 89124073 89123987 ID=MsG0480023731.01.T01:exon:12452;Parent=MsG0480023731.01.T01
Chr4 exon 89124205 89124378 89124205 ID=MsG0480023731.01.T01:exon:12451;Parent=MsG0480023731.01.T01
Chr4 five_prime_UTR 89124227 89124378 89124227 ID=MsG0480023731.01.T01:five_prime_utr;Parent=MsG0480023731.01.T01
Chr4 CDS 89124205 89124226 89124205 ID=MsG0480023731.01.T01:cds;Parent=MsG0480023731.01.T01
Chr4 CDS 89123987 89124073 89123987 ID=MsG0480023731.01.T01:cds;Parent=MsG0480023731.01.T01
Chr4 CDS 89123384 89123661 89123384 ID=MsG0480023731.01.T01:cds;Parent=MsG0480023731.01.T01
Chr4 CDS 89122963 89123139 89122963 ID=MsG0480023731.01.T01:cds;Parent=MsG0480023731.01.T01
Chr4 three_prime_UTR 89122827 89122962 89122827 ID=MsG0480023731.01.T01:three_prime_utr;Parent=MsG0480023731.01.T01
Gene Sequence

>MsG0480023731.01.T01

ATGAATTCTTCTACTTCTACAGCCAAAGAAACTGCAACTGCTAAAATGGAGCACAAGAAAGCAACAACCAGGAAAACCAGAAGTTTTGCATGTAGGATTCATGAACATGTAAAACTAGGTCCTAAACTATCAGAGACTCTGAAGGGTAAGTTGAGTTTGGGTGCAAGGATAATACAAGAAGGTGGGAGAGGGAACATTTTCAAGAATATTTTTGGTATGCAAGAAGGAGAGAAAGTGTTGAAGGCTTCACAATGTTACTTATACACAACAGCTGGTCCTATTGCTGGAGTTCTTTTCATCTCCACCCAAAAAGTTGCATTTTGTAGTGAAAGACCAATAACCTTCTCTTCTGCATCAACGGGAGAGACGCGCAAGATACCGTACAAGGTTTTGATACCTATTGGGAAGATAAAAGAAGTCAATGAAGGGCAGAATGTGAATCAGGAAGAACAAAAGTATATAGAAATTGTTACTCACGATGATTCTGAATTTTGGTTTATGGGATTTTTACGCTATGAGAAAGCTTTTAGGAATCTTCAAAAAGCCATTTCAATGTCCATTTAG

Protein sequence

>MsG0480023731.01.T01

MNSSTSTAKETATAKMEHKKATTRKTRSFACRIHEHVKLGPKLSETLKGKLSLGARIIQEGGRGNIFKNIFGMQEGEKVLKASQCYLYTTAGPIAGVLFISTQKVAFCSERPITFSSASTGETRKIPYKVLIPIGKIKEVNEGQNVNQEEQKYIEIVTHDDSEFWFMGFLRYEKAFRNLQKAISMSI*