Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480023793.01.T01 | XP_024628281.1 | 99.248 | 133 | 1 | 0 | 1 | 133 | 1 | 133 | 1.16E-91 | 271 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480023793.01.T01 | A4K9Z8 | 90.226 | 133 | 13 | 0 | 1 | 133 | 1 | 133 | 7.70E-88 | 254 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480023793.01.T01 | A0A396GR17 | 99.248 | 133 | 1 | 0 | 1 | 133 | 1 | 133 | 5.55e-92 | 271 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|---|---|
| MsG0480023793.01 | MsG0680035804.01 | PPI |
| MsG0480023794.01 | MsG0480023793.01 | PPI |
| MsG0780037052.01 | MsG0480023793.01 | PPI |
| MsG0480023220.01 | MsG0480023793.01 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480023793.01.T01 | MTR_6g090030 | 89.474 | 133 | 14 | 0 | 1 | 133 | 1 | 133 | 6.44e-88 | 252 |
| MsG0480023793.01.T01 | MTR_8g102330 | 84.211 | 133 | 21 | 0 | 1 | 133 | 1 | 133 | 1.99e-83 | 240 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480023793.01.T01 | AT2G19770 | 86.567 | 134 | 17 | 1 | 1 | 133 | 1 | 134 | 1.43e-85 | 246 |
| MsG0480023793.01.T01 | AT4G29340 | 84.328 | 134 | 20 | 1 | 1 | 133 | 1 | 134 | 2.56e-83 | 240 |
| MsG0480023793.01.T01 | AT2G19760 | 75.188 | 133 | 31 | 1 | 1 | 133 | 1 | 131 | 2.11e-71 | 210 |
| MsG0480023793.01.T01 | AT4G29350 | 73.684 | 133 | 33 | 1 | 1 | 133 | 1 | 131 | 1.23e-70 | 208 |
| MsG0480023793.01.T01 | AT5G56600 | 72.180 | 133 | 35 | 1 | 1 | 133 | 1 | 131 | 6.97e-69 | 204 |
| MsG0480023793.01.T01 | AT5G56600 | 70.787 | 89 | 24 | 1 | 1 | 89 | 1 | 87 | 3.10e-41 | 133 |
Find 50 sgRNAs with CRISPR-Local
Find 78 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCATCTGTTTCTTTCTTAAT+TGG | 0.128837 | 4:+89897713 | MsG0480023793.01.T01:three_prime_UTR |
| TATGCAACAACTCCCGATTC+AGG | 0.266052 | 4:-89897798 | None:intergenic |
| TTGGCCATGATGGCTCTGTT+TGG | 0.298573 | 4:+89896582 | MsG0480023793.01.T01:CDS |
| CCTACGGGCTTGCACCTTGC+AGG | 0.315777 | 4:+89897218 | MsG0480023793.01.T01:CDS |
| TGGCCATGATGGCTCTGTTT+GGG | 0.344130 | 4:+89896583 | MsG0480023793.01.T01:CDS |
| CTATCTGCCGCTGCCATCAT+TGG | 0.366810 | 4:+89896563 | MsG0480023793.01.T01:CDS |
| ATGGTCATCCAAGGAGAGTC+TGG | 0.390726 | 4:+89897251 | MsG0480023793.01.T01:CDS |
| GGGAGATTACCTCATTGATC+AGG | 0.391286 | 4:+89897659 | MsG0480023793.01.T01:CDS |
| CATAATAAAATCTTATTGAA+TGG | 0.398488 | 4:+89897886 | MsG0480023793.01.T01:three_prime_UTR |
| ATCACATTGCAACCCTGAAT+CGG | 0.418082 | 4:+89897785 | MsG0480023793.01.T01:three_prime_UTR |
| TTGTTATTTGGATAGGGATC+TGG | 0.425299 | 4:+89897540 | MsG0480023793.01.T01:intron |
| CAATGATGGCAGCGGCAGAT+AGG | 0.436163 | 4:-89896562 | None:intergenic |
| ACCTGGTCATCTTGCTCCTA+CGG | 0.438799 | 4:+89897202 | MsG0480023793.01.T01:CDS |
| ATCATAGAGTCTGTGTCTAC+AGG | 0.447469 | 4:-89897683 | None:intergenic |
| TCACATTGCAACCCTGAATC+GGG | 0.465748 | 4:+89897786 | MsG0480023793.01.T01:three_prime_UTR |
| TGACAGCTCCAGACTCTCCT+TGG | 0.476205 | 4:-89897259 | None:intergenic |
| AGCGGCAGATAGGTGGTGTC+CGG | 0.488939 | 4:-89896552 | None:intergenic |
| CCTGGTCATCTTGCTCCTAC+GGG | 0.490248 | 4:+89897203 | MsG0480023793.01.T01:CDS |
| GCTGCCATCATTGGCCATGA+TGG | 0.491650 | 4:+89896572 | MsG0480023793.01.T01:CDS |
| GGCATCACCATAAAGAAAAC+CGG | 0.492694 | 4:+89897564 | MsG0480023793.01.T01:CDS |
| CATTTGATGTGTGATATTGA+TGG | 0.492739 | 4:+89896527 | MsG0480023793.01.T01:CDS |
| TTGCATTGTCCAGGAGTCAC+AGG | 0.497282 | 4:-89897618 | None:intergenic |
| ATGCAACAACTCCCGATTCA+GGG | 0.499490 | 4:-89897797 | None:intergenic |
| CTTCTTGTTGTGTAGTGTTG+TGG | 0.499929 | 4:-89896449 | None:intergenic |
| ACCGAAAACTAGAGCTTGAC+CGG | 0.513054 | 4:-89897583 | None:intergenic |
| CAACATGGTTGTTGAGAGGT+TGG | 0.513380 | 4:+89897638 | MsG0480023793.01.T01:CDS |
| ACAACCATGTTGCATTGTCC+AGG | 0.519180 | 4:-89897627 | None:intergenic |
| AGCTGTCATCCGTGGGAAGA+AGG | 0.522760 | 4:+89897274 | MsG0480023793.01.T01:CDS |
| TATGAGGAACCTGTGACTCC+TGG | 0.523087 | 4:+89897609 | MsG0480023793.01.T01:CDS |
| GAGCCCAAACAGAGCCATCA+TGG | 0.526888 | 4:-89896586 | None:intergenic |
| CCCGTAGGAGCAAGATGACC+AGG | 0.528322 | 4:-89897203 | None:intergenic |
| ATAATATCAGTGATCTCTTG+AGG | 0.540089 | 4:-89897164 | None:intergenic |
| AGAGCCATCATGGCCAATGA+TGG | 0.541950 | 4:-89896576 | None:intergenic |
| AACATGGTTGTTGAGAGGTT+GGG | 0.543359 | 4:+89897639 | MsG0480023793.01.T01:CDS |
| GAGTCTGGAGCTGTCATCCG+TGG | 0.545615 | 4:+89897266 | MsG0480023793.01.T01:CDS |
| GTCAAATACATGGTCATCCA+AGG | 0.546015 | 4:+89897242 | MsG0480023793.01.T01:CDS |
| TTATTTGGATAGGGATCTGG+AGG | 0.562519 | 4:+89897543 | MsG0480023793.01.T01:intron |
| AATAATTTACCTTCTTCCCA+CGG | 0.573845 | 4:-89897283 | None:intergenic |
| GGAGATTACCTCATTGATCA+GGG | 0.584642 | 4:+89897660 | MsG0480023793.01.T01:CDS |
| CCTGCAAGGTGCAAGCCCGT+AGG | 0.594563 | 4:-89897218 | None:intergenic |
| ATGTGTGATATTGATGGCAC+CGG | 0.603576 | 4:+89896533 | MsG0480023793.01.T01:CDS |
| CCTTGCAGGAGTCAAATACA+TGG | 0.605937 | 4:+89897232 | MsG0480023793.01.T01:CDS |
| ATCATGGCCAATGATGGCAG+CGG | 0.611873 | 4:-89896570 | None:intergenic |
| TCATGAATATACTTGAGGAA+AGG | 0.612766 | 4:-89896618 | None:intergenic |
| TGATGGCAGCGGCAGATAGG+TGG | 0.614292 | 4:-89896559 | None:intergenic |
| GACTCCTGGACAATGCAACA+TGG | 0.634894 | 4:+89897623 | MsG0480023793.01.T01:CDS |
| AGTCTGGAGCTGTCATCCGT+GGG | 0.647697 | 4:+89897267 | MsG0480023793.01.T01:CDS |
| AATGCAACATGGTTGTTGAG+AGG | 0.662368 | 4:+89897634 | MsG0480023793.01.T01:CDS |
| TCTACAGGCCCTGATCAATG+AGG | 0.683059 | 4:-89897668 | None:intergenic |
| CCATGTATTTGACTCCTGCA+AGG | 0.700396 | 4:-89897232 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTATCTTTTCATTTTTATA+TGG | + | Chr4:89897753-89897772 | MsG0480023793.01.T01:three_prime_UTR | 10.0% |
| !! | AAACAACATTGTAATTAAAA+TGG | - | Chr4:89896925-89896944 | None:intergenic | 15.0% |
| !! | CATAATAAAATCTTATTGAA+TGG | + | Chr4:89897886-89897905 | MsG0480023793.01.T01:three_prime_UTR | 15.0% |
| !! | ATCATCATTGTAAAGATTTA+CGG | - | Chr4:89896858-89896877 | None:intergenic | 20.0% |
| !!! | AAAAAAGGAACAAATTTTGA+AGG | + | Chr4:89896472-89896491 | MsG0480023793.01.T01:five_prime_UTR | 20.0% |
| ! | GTTGAAGATTAGATTTGATT+AGG | + | Chr4:89897064-89897083 | MsG0480023793.01.T01:intron | 25.0% |
| ! | TGTAATCATGAATATACTTG+AGG | - | Chr4:89896626-89896645 | None:intergenic | 25.0% |
| !! | TATGTAAGATCGATTTAGAA+AGG | - | Chr4:89897003-89897022 | None:intergenic | 25.0% |
| !! | TCATCTGTTTCTTTCTTAAT+TGG | + | Chr4:89897713-89897732 | MsG0480023793.01.T01:three_prime_UTR | 25.0% |
| !!! | TGAAAAATGCATGTTAGTTT+TGG | + | Chr4:89896896-89896915 | MsG0480023793.01.T01:intron | 25.0% |
| !!! | TGATATATGTGCTTGTTATT+TGG | + | Chr4:89897528-89897547 | MsG0480023793.01.T01:intron | 25.0% |
| AAAAAAGAGACATGTACGTT+AGG | - | Chr4:89897489-89897508 | None:intergenic | 30.0% | |
| AAAAGTTAGTCCATAAGAAG+AGG | - | Chr4:89897415-89897434 | None:intergenic | 30.0% | |
| AAAGTTAGTCCATAAGAAGA+GGG | - | Chr4:89897414-89897433 | None:intergenic | 30.0% | |
| AATAATTTACCTTCTTCCCA+CGG | - | Chr4:89897286-89897305 | None:intergenic | 30.0% | |
| ATAATATCAGTGATCTCTTG+AGG | - | Chr4:89897167-89897186 | None:intergenic | 30.0% | |
| ATGCATAGAATAGTTTCATG+TGG | - | Chr4:89896652-89896671 | None:intergenic | 30.0% | |
| CATTTGATGTGTGATATTGA+TGG | + | Chr4:89896527-89896546 | MsG0480023793.01.T01:CDS | 30.0% | |
| CTACACAACAAGAAGAAAAA+AGG | + | Chr4:89896457-89896476 | MsG0480023793.01.T01:five_prime_UTR | 30.0% | |
| GAAGATTAGATTTGATTAGG+AGG | + | Chr4:89897067-89897086 | MsG0480023793.01.T01:intron | 30.0% | |
| TCATGAATATACTTGAGGAA+AGG | - | Chr4:89896621-89896640 | None:intergenic | 30.0% | |
| TGCATAGAATAGTTTCATGT+GGG | - | Chr4:89896651-89896670 | None:intergenic | 30.0% | |
| TTCAAAATCACACTATGATC+TGG | - | Chr4:89896781-89896800 | None:intergenic | 30.0% | |
| !! | ATGTGCTTGTTATTTGGATA+GGG | + | Chr4:89897534-89897553 | MsG0480023793.01.T01:intron | 30.0% |
| !! | TATGTGCTTGTTATTTGGAT+AGG | + | Chr4:89897533-89897552 | MsG0480023793.01.T01:intron | 30.0% |
| GCATAGATCTCTATCATTCT+TGG | + | Chr4:89896668-89896687 | MsG0480023793.01.T01:intron | 35.0% | |
| GTTGTATTTGAGTCTCATGT+TGG | + | Chr4:89897345-89897364 | MsG0480023793.01.T01:intron | 35.0% | |
| !! | ATGAAAGATTTTGACGAACC+TGG | + | Chr4:89897185-89897204 | MsG0480023793.01.T01:CDS | 35.0% |
| !! | TACGTTAGGTTTTATGCATG+TGG | - | Chr4:89897475-89897494 | None:intergenic | 35.0% |
| !! | TTGTTATTTGGATAGGGATC+TGG | + | Chr4:89897540-89897559 | MsG0480023793.01.T01:intron | 35.0% |
| !! | TTTTGAAGGACAAAATGTCG+TGG | + | Chr4:89896486-89896505 | MsG0480023793.01.T01:exon | 35.0% |
| !!! | TCTAGTTTTCGGTGTTTATG+AGG | + | Chr4:89897593-89897612 | MsG0480023793.01.T01:CDS | 35.0% |
| AACATGGTTGTTGAGAGGTT+GGG | + | Chr4:89897639-89897658 | MsG0480023793.01.T01:CDS | 40.0% | |
| AATGCAACATGGTTGTTGAG+AGG | + | Chr4:89897634-89897653 | MsG0480023793.01.T01:CDS | 40.0% | |
| ATCACATTGCAACCCTGAAT+CGG | + | Chr4:89897785-89897804 | MsG0480023793.01.T01:three_prime_UTR | 40.0% | |
| ATCATAGAGTCTGTGTCTAC+AGG | - | Chr4:89897686-89897705 | None:intergenic | 40.0% | |
| GGAGATTACCTCATTGATCA+GGG | + | Chr4:89897660-89897679 | MsG0480023793.01.T01:CDS | 40.0% | |
| GGCATCACCATAAAGAAAAC+CGG | + | Chr4:89897564-89897583 | MsG0480023793.01.T01:CDS | 40.0% | |
| GTCAAATACATGGTCATCCA+AGG | + | Chr4:89897242-89897261 | MsG0480023793.01.T01:CDS | 40.0% | |
| GTTTGAGTACCCTCTTCTTA+TGG | + | Chr4:89897402-89897421 | MsG0480023793.01.T01:intron | 40.0% | |
| TTATTTGGATAGGGATCTGG+AGG | + | Chr4:89897543-89897562 | MsG0480023793.01.T01:intron | 40.0% | |
| ! | ATGTGTGATATTGATGGCAC+CGG | + | Chr4:89896533-89896552 | MsG0480023793.01.T01:CDS | 40.0% |
| ! | CTTCTTGTTGTGTAGTGTTG+TGG | - | Chr4:89896452-89896471 | None:intergenic | 40.0% |
| !! | AGCTTGACCGGTTTTCTTTA+TGG | - | Chr4:89897574-89897593 | None:intergenic | 40.0% |
| ACAACCATGTTGCATTGTCC+AGG | - | Chr4:89897630-89897649 | None:intergenic | 45.0% | |
| ACCGAAAACTAGAGCTTGAC+CGG | - | Chr4:89897586-89897605 | None:intergenic | 45.0% | |
| ATGCAACAACTCCCGATTCA+GGG | - | Chr4:89897800-89897819 | None:intergenic | 45.0% | |
| CAACATGGTTGTTGAGAGGT+TGG | + | Chr4:89897638-89897657 | MsG0480023793.01.T01:CDS | 45.0% | |
| CCATGTATTTGACTCCTGCA+AGG | - | Chr4:89897235-89897254 | None:intergenic | 45.0% | |
| CCTTGCAGGAGTCAAATACA+TGG | + | Chr4:89897232-89897251 | MsG0480023793.01.T01:CDS | 45.0% | |
| GGGAGATTACCTCATTGATC+AGG | + | Chr4:89897659-89897678 | MsG0480023793.01.T01:CDS | 45.0% | |
| TATGCAACAACTCCCGATTC+AGG | - | Chr4:89897801-89897820 | None:intergenic | 45.0% | |
| TCACATTGCAACCCTGAATC+GGG | + | Chr4:89897786-89897805 | MsG0480023793.01.T01:three_prime_UTR | 45.0% | |
| !!! | ACCGGTCAAGCTCTAGTTTT+CGG | + | Chr4:89897582-89897601 | MsG0480023793.01.T01:CDS | 45.0% |
| ACCTGGTCATCTTGCTCCTA+CGG | + | Chr4:89897202-89897221 | MsG0480023793.01.T01:CDS | 50.0% | |
| AGAGCCATCATGGCCAATGA+TGG | - | Chr4:89896579-89896598 | None:intergenic | 50.0% | |
| ATCATGGCCAATGATGGCAG+CGG | - | Chr4:89896573-89896592 | None:intergenic | 50.0% | |
| ATGGTCATCCAAGGAGAGTC+TGG | + | Chr4:89897251-89897270 | MsG0480023793.01.T01:CDS | 50.0% | |
| GACTCCTGGACAATGCAACA+TGG | + | Chr4:89897623-89897642 | MsG0480023793.01.T01:CDS | 50.0% | |
| TATGAGGAACCTGTGACTCC+TGG | + | Chr4:89897609-89897628 | MsG0480023793.01.T01:CDS | 50.0% | |
| TCTACAGGCCCTGATCAATG+AGG | - | Chr4:89897671-89897690 | None:intergenic | 50.0% | |
| TTGCATTGTCCAGGAGTCAC+AGG | - | Chr4:89897621-89897640 | None:intergenic | 50.0% | |
| ! | TGGCCATGATGGCTCTGTTT+GGG | + | Chr4:89896583-89896602 | MsG0480023793.01.T01:CDS | 50.0% |
| ! | TTGGCCATGATGGCTCTGTT+TGG | + | Chr4:89896582-89896601 | MsG0480023793.01.T01:CDS | 50.0% |
| AGCTGTCATCCGTGGGAAGA+AGG | + | Chr4:89897274-89897293 | MsG0480023793.01.T01:CDS | 55.0% | |
| CAATGATGGCAGCGGCAGAT+AGG | - | Chr4:89896565-89896584 | None:intergenic | 55.0% | |
| CCTGGTCATCTTGCTCCTAC+GGG | + | Chr4:89897203-89897222 | MsG0480023793.01.T01:CDS | 55.0% | |
| CTATCTGCCGCTGCCATCAT+TGG | + | Chr4:89896563-89896582 | MsG0480023793.01.T01:CDS | 55.0% | |
| GAGCCCAAACAGAGCCATCA+TGG | - | Chr4:89896589-89896608 | None:intergenic | 55.0% | |
| GCTGCCATCATTGGCCATGA+TGG | + | Chr4:89896572-89896591 | MsG0480023793.01.T01:CDS | 55.0% | |
| TGACAGCTCCAGACTCTCCT+TGG | - | Chr4:89897262-89897281 | None:intergenic | 55.0% | |
| ! | AGTCTGGAGCTGTCATCCGT+GGG | + | Chr4:89897267-89897286 | MsG0480023793.01.T01:CDS | 55.0% |
| AGCGGCAGATAGGTGGTGTC+CGG | - | Chr4:89896555-89896574 | None:intergenic | 60.0% | |
| CCCGTAGGAGCAAGATGACC+AGG | - | Chr4:89897206-89897225 | None:intergenic | 60.0% | |
| TGATGGCAGCGGCAGATAGG+TGG | - | Chr4:89896562-89896581 | None:intergenic | 60.0% | |
| ! | GAGTCTGGAGCTGTCATCCG+TGG | + | Chr4:89897266-89897285 | MsG0480023793.01.T01:CDS | 60.0% |
| CCTACGGGCTTGCACCTTGC+AGG | + | Chr4:89897218-89897237 | MsG0480023793.01.T01:CDS | 65.0% | |
| CCTGCAAGGTGCAAGCCCGT+AGG | - | Chr4:89897221-89897240 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 89896445 | 89897913 | 89896445 | ID=MsG0480023793.01;Name=MsG0480023793.01 |
| Chr4 | mRNA | 89896445 | 89897913 | 89896445 | ID=MsG0480023793.01.T01;Parent=MsG0480023793.01;Name=MsG0480023793.01.T01;_AED=0.50;_eAED=0.50;_QI=55|1|1|1|1|1|3|224|133 |
| Chr4 | exon | 89896445 | 89896628 | 89896445 | ID=MsG0480023793.01.T01:exon:25374;Parent=MsG0480023793.01.T01 |
| Chr4 | exon | 89897158 | 89897295 | 89897158 | ID=MsG0480023793.01.T01:exon:25375;Parent=MsG0480023793.01.T01 |
| Chr4 | exon | 89897555 | 89897913 | 89897555 | ID=MsG0480023793.01.T01:exon:25376;Parent=MsG0480023793.01.T01 |
| Chr4 | five_prime_UTR | 89896445 | 89896499 | 89896445 | ID=MsG0480023793.01.T01:five_prime_utr;Parent=MsG0480023793.01.T01 |
| Chr4 | CDS | 89896500 | 89896628 | 89896500 | ID=MsG0480023793.01.T01:cds;Parent=MsG0480023793.01.T01 |
| Chr4 | CDS | 89897158 | 89897295 | 89897158 | ID=MsG0480023793.01.T01:cds;Parent=MsG0480023793.01.T01 |
| Chr4 | CDS | 89897555 | 89897689 | 89897555 | ID=MsG0480023793.01.T01:cds;Parent=MsG0480023793.01.T01 |
| Chr4 | three_prime_UTR | 89897690 | 89897913 | 89897690 | ID=MsG0480023793.01.T01:three_prime_utr;Parent=MsG0480023793.01.T01 |
| Gene Sequence |
| Protein sequence |
