Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780037052.01.T01 | XP_003622019.1 | 100 | 377 | 0 | 0 | 1 | 377 | 1 | 377 | 0 | 784 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048253.01 | MsG0780037052.01 | 0.837968 | 3.799271e-57 | 5.016653e-54 |
| MsG0080048717.01 | MsG0780037052.01 | 0.818847 | 1.582803e-52 | 1.198319e-49 |
| MsG0080048748.01 | MsG0780037052.01 | -0.849196 | 3.775411e-60 | 7.102749e-57 |
| MsG0080048849.01 | MsG0780037052.01 | -0.802413 | 5.677988e-49 | 2.779676e-46 |
| MsG0080048938.01 | MsG0780037052.01 | -0.892567 | 1.409495e-74 | 1.295793e-70 |
| MsG0080049069.01 | MsG0780037052.01 | -0.820066 | 8.344775e-53 | 6.535700e-50 |
| MsG0080049070.01 | MsG0780037052.01 | -0.823229 | 1.549985e-53 | 1.326704e-50 |
| MsG0180000011.01 | MsG0780037052.01 | 0.823598 | 1.270779e-53 | 1.099156e-50 |
| MsG0180000017.01 | MsG0780037052.01 | -0.813010 | 3.178285e-51 | 2.051532e-48 |
| MsG0180000246.01 | MsG0780037052.01 | -0.870061 | 1.939075e-66 | 7.441404e-63 |
PPI
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780037052.01.T01 | MTR_7g026230 | 100.000 | 377 | 0 | 0 | 1 | 377 | 1 | 377 | 0.0 | 784 |
| MsG0780037052.01.T01 | MTR_6g004000 | 97.082 | 377 | 11 | 0 | 1 | 377 | 1 | 377 | 0.0 | 769 |
| MsG0780037052.01.T01 | MTR_0015s0130 | 97.613 | 377 | 9 | 0 | 1 | 377 | 1 | 377 | 0.0 | 768 |
| MsG0780037052.01.T01 | MTR_2g096840 | 97.082 | 377 | 11 | 0 | 1 | 377 | 1 | 377 | 0.0 | 768 |
| MsG0780037052.01.T01 | MTR_3g095530 | 96.021 | 377 | 15 | 0 | 1 | 377 | 1 | 377 | 0.0 | 762 |
| MsG0780037052.01.T01 | MTR_7g093260 | 96.021 | 377 | 15 | 0 | 1 | 377 | 1 | 377 | 0.0 | 761 |
| MsG0780037052.01.T01 | MTR_2g008050 | 93.899 | 377 | 23 | 0 | 1 | 377 | 1 | 377 | 0.0 | 751 |
| MsG0780037052.01.T01 | MTR_7g027185 | 43.272 | 379 | 201 | 3 | 10 | 374 | 7 | 385 | 3.11e-120 | 353 |
| MsG0780037052.01.T01 | MTR_7g027185 | 44.507 | 355 | 184 | 2 | 10 | 351 | 7 | 361 | 1.17e-117 | 346 |
| MsG0780037052.01.T01 | MTR_7g027185 | 47.557 | 307 | 159 | 1 | 10 | 314 | 7 | 313 | 5.91e-110 | 324 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780037052.01.T01 | AT3G12110 | 97.613 | 377 | 9 | 0 | 1 | 377 | 1 | 377 | 0.0 | 770 |
| MsG0780037052.01.T01 | AT3G53750 | 96.286 | 377 | 14 | 0 | 1 | 377 | 1 | 377 | 0.0 | 762 |
| MsG0780037052.01.T01 | AT3G53750 | 96.286 | 377 | 14 | 0 | 1 | 377 | 1 | 377 | 0.0 | 762 |
| MsG0780037052.01.T01 | AT2G37620 | 96.286 | 377 | 14 | 0 | 1 | 377 | 1 | 377 | 0.0 | 762 |
| MsG0780037052.01.T01 | AT2G37620 | 96.286 | 377 | 14 | 0 | 1 | 377 | 1 | 377 | 0.0 | 762 |
| MsG0780037052.01.T01 | AT2G37620 | 96.286 | 377 | 14 | 0 | 1 | 377 | 1 | 377 | 0.0 | 762 |
| MsG0780037052.01.T01 | AT2G37620 | 96.286 | 377 | 14 | 0 | 1 | 377 | 1 | 377 | 0.0 | 762 |
| MsG0780037052.01.T01 | AT3G46520 | 96.286 | 377 | 14 | 0 | 1 | 377 | 1 | 377 | 0.0 | 758 |
| MsG0780037052.01.T01 | AT3G46520 | 96.286 | 377 | 14 | 0 | 1 | 377 | 1 | 377 | 0.0 | 758 |
| MsG0780037052.01.T01 | AT5G09810 | 95.225 | 377 | 18 | 0 | 1 | 377 | 1 | 377 | 0.0 | 756 |
Find 141 sgRNAs with CRISPR-Local
Find 276 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAAGCACTGGGTGTTCTTC+TGG | 0.181767 | 7:-18603147 | None:intergenic |
| AAAATGCCGAGGGTGATGGA+AGG | 0.638138 | 7:-18601020 | None:intergenic |
| AAAGCACTGGGTGTTCTTCT+GGG | 0.361175 | 7:-18603146 | None:intergenic |
| AAATACAGTGTCTGGATTGG+AGG | 0.490757 | 7:+18604457 | MsG0780037052.01.T01:CDS |
| AACAAAACAAACCTGCTGGA+AGG | 0.616042 | 7:-18604503 | None:intergenic |
| AACAATTTCTCGCTCTGCTG+AGG | 0.553097 | 7:-18604056 | None:intergenic |
| AACATGGAATCGTTAGCAAC+TGG | 0.386131 | 7:+18603070 | MsG0780037052.01.T01:CDS |
| AAGAGCTACGAATTGCCTGA+TGG | 0.595436 | 7:+18604163 | MsG0780037052.01.T01:CDS |
| AAGCACTGGGTGTTCTTCTG+GGG | 0.572123 | 7:-18603145 | None:intergenic |
| AAGGAAAATGCCGAGGGTGA+TGG | 0.500817 | 7:-18601024 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAACTTAATGCTATCAACCA+TGG | - | Chr7:18602830-18602849 | None:intergenic | 30.0% | |
| AAGTTATGCTAGAGAACTAA+TGG | - | Chr7:18604768-18604787 | None:intergenic | 30.0% | |
| AGAAAGAATAGATTCCGAAA+AGG | + | Chr7:18601894-18601913 | MsG0780037052.01.T01:intron | 30.0% | |
| AGGTAAAAAGTGAAATTTGG+TGG | + | Chr7:18601854-18601873 | MsG0780037052.01.T01:intron | 30.0% | |
| ATTAAAAAATGACTTACCGG+TGG | - | Chr7:18603301-18603320 | None:intergenic | 30.0% | |
| ATTCTGCGTATGAATTAACT+GGG | + | Chr7:18605346-18605365 | MsG0780037052.01.T01:three_prime_UTR | 30.0% | |
| CAAAAACACAAACACAATGT+TGG | - | Chr7:18601679-18601698 | None:intergenic | 30.0% | |
| GACTAGGTGTAATGTATATT+TGG | + | Chr7:18602007-18602026 | MsG0780037052.01.T01:intron | 30.0% | |
| GAGGAAATGAAAATGGAAAA+TGG | - | Chr7:18600907-18600926 | None:intergenic | 30.0% | |
| GAGTCTAACAAACAAAAAAG+AGG | - | Chr7:18600926-18600945 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 18600816 | 18605431 | 18600816 | ID=MsG0780037052.01;Name=MsG0780037052.01 |
| Chr7 | mRNA | 18600816 | 18605431 | 18600816 | ID=MsG0780037052.01.T01;Parent=MsG0780037052.01;Name=MsG0780037052.01.T01;_AED=0.32;_eAED=0.32;_QI=248|1|1|1|0.75|0.6|5|319|377 |
| Chr7 | exon | 18600816 | 18601053 | 18600816 | ID=MsG0780037052.01.T01:exon:2990;Parent=MsG0780037052.01.T01 |
| Chr7 | exon | 18602347 | 18602416 | 18602347 | ID=MsG0780037052.01.T01:exon:2991;Parent=MsG0780037052.01.T01 |
| Chr7 | exon | 18602910 | 18603303 | 18602910 | ID=MsG0780037052.01.T01:exon:2992;Parent=MsG0780037052.01.T01 |
| Chr7 | exon | 18603900 | 18604513 | 18603900 | ID=MsG0780037052.01.T01:exon:2993;Parent=MsG0780037052.01.T01 |
| Chr7 | exon | 18605047 | 18605431 | 18605047 | ID=MsG0780037052.01.T01:exon:2994;Parent=MsG0780037052.01.T01 |
| Chr7 | five_prime_UTR | 18600816 | 18601053 | 18600816 | ID=MsG0780037052.01.T01:five_prime_utr;Parent=MsG0780037052.01.T01 |
| Chr7 | five_prime_UTR | 18602347 | 18602356 | 18602347 | ID=MsG0780037052.01.T01:five_prime_utr;Parent=MsG0780037052.01.T01 |
| Chr7 | CDS | 18602357 | 18602416 | 18602357 | ID=MsG0780037052.01.T01:cds;Parent=MsG0780037052.01.T01 |
| Chr7 | CDS | 18602910 | 18603303 | 18602910 | ID=MsG0780037052.01.T01:cds;Parent=MsG0780037052.01.T01 |
| Chr7 | CDS | 18603900 | 18604513 | 18603900 | ID=MsG0780037052.01.T01:cds;Parent=MsG0780037052.01.T01 |
| Chr7 | CDS | 18605047 | 18605112 | 18605047 | ID=MsG0780037052.01.T01:cds;Parent=MsG0780037052.01.T01 |
| Chr7 | three_prime_UTR | 18605113 | 18605431 | 18605113 | ID=MsG0780037052.01.T01:three_prime_utr;Parent=MsG0780037052.01.T01 |
| Gene Sequence |
>MsG0780037052.01.T01 ATGGCAGACGCTGAGGATATTCAACCTCTTGTTTGTGACAATGGAACTGGAATGGTTAAGGCTGGATTTGCTGGAGATGATGCCCCTCGTGCTGTGTTTCCCAGCATTGTAGGTCGTCCTCGTCACACTGGTGTGATGGTTGGCATGGGTCAAAAAGATGCATATGTTGGTGATGAGGCACAGTCTAAGAGGGGTATTTTGACTCTCAAATACCCCATTGAACATGGAATCGTTAGCAACTGGGACGACATGGAAAAAATTTGGCATCACACCTTCTACAATGAGCTTCGTGTCGCCCCAGAAGAACACCCAGTGCTTTTAACTGAGGCTCCTCTCAACCCTAAGGCCAATCGTGAGAAGATGACTCAAATCATGTTTGAGACTTTTAATACCCCTGCCATGTATGTTGCTATTCAGGCCGTTCTTTCACTATACGCCAGTGGTCGTACCACCGGTATTGTGTTGGATTCTGGTGATGGTGTCAGCCATACTGTCCCTATCTACGAGGGTTATGCCCTTCCACATGCCATCCTTCGTCTTGATCTGGCTGGTCGCGATCTTACCGATTTCTTGATGAAGATTTTGACTGAACGTGGTTATTCTTTTACCACCTCAGCAGAGCGAGAAATTGTTCGTGACGTGAAAGAAAAGCTTTCATACATTGCCCTTGACTATGAGCAAGAGCTCGAGACAGCCAGGACCAGCTCATCCGTCGAGAAGAGCTACGAATTGCCTGATGGACAGGTGATCACCATCGGAGACGAGCGTTTCAGATGTCCGGAGGTCCTGTTCCAACCATCTATGATAGGAATGGAAGCAGCAGGCATTCACGAGACCACATACAACTCCATCATGAAGTGCGATGTTGATATCAGGAAAGACTTGTACGGTAACATTGTGCTTTCAGGAGGAACAACCATGTTCCCAGGCATTGCTGACAGAATGAGCAAGGAAATTTCTGCTTTGGCCCCAAGTAGCATGAAGATCAAGGTGGTTGCACCACCTGAGAGAAAATACAGTGTCTGGATTGGAGGTTCCATCTTGGCATCTCTTAGCACCTTCCAGCAGATGTGGATTGCAAAGGCAGAATATGATGAATCTGGTCCATCAATTGTCCACAGAAAGTGCTTCTAA |
| Protein sequence |
>MsG0780037052.01.T01 MADAEDIQPLVCDNGTGMVKAGFAGDDAPRAVFPSIVGRPRHTGVMVGMGQKDAYVGDEAQSKRGILTLKYPIEHGIVSNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDFLMKILTERGYSFTTSAEREIVRDVKEKLSYIALDYEQELETARTSSSVEKSYELPDGQVITIGDERFRCPEVLFQPSMIGMEAAGIHETTYNSIMKCDVDIRKDLYGNIVLSGGTTMFPGIADRMSKEISALAPSSMKIKVVAPPERKYSVWIGGSILASLSTFQQMWIAKAEYDESGPSIVHRKCF* |
