Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480023870.01.T01 | XP_003602582.1 | 84.615 | 78 | 12 | 0 | 3 | 80 | 85 | 162 | 4.76E-39 | 137 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480023870.01.T01 | G7JBH3 | 84.615 | 78 | 12 | 0 | 3 | 80 | 85 | 162 | 2.28e-39 | 137 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000118.01 | MsG0480023870.01 | 0.805920 | 1.057318e-49 | 5.663586e-47 |
| MsG0480022789.01 | MsG0480023870.01 | 0.812083 | 5.068246e-51 | 3.191709e-48 |
| MsG0380015446.01 | MsG0480023870.01 | 0.814398 | 1.572629e-51 | 1.054115e-48 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480023870.01.T01 | MTR_3g095900 | 84.615 | 78 | 12 | 0 | 3 | 80 | 85 | 162 | 5.77e-43 | 137 |
| MsG0480023870.01.T01 | MTR_8g103900 | 82.051 | 78 | 14 | 0 | 3 | 80 | 85 | 162 | 1.42e-40 | 130 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480023870.01.T01 | AT5G64650 | 72.727 | 77 | 20 | 1 | 3 | 79 | 85 | 160 | 5.62e-34 | 114 |
| MsG0480023870.01.T01 | AT5G09770 | 71.429 | 77 | 21 | 1 | 3 | 79 | 85 | 160 | 8.32e-33 | 111 |
Find 27 sgRNAs with CRISPR-Local
Find 44 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTCTGCGGTGGTTGAGGAGT+TGG | 0.413679 | 4:-90693898 | None:intergenic |
| GGTGCTCTCTGCGGTGGTTG+AGG | 0.432915 | 4:-90693904 | None:intergenic |
| CAGAGAGCACCCTTGATCCC+TGG | 0.484278 | 4:+90693916 | MsG0480023870.01.T01:CDS |
| GAGATCGTGTCCAGGGATCA+AGG | 0.491136 | 4:-90693926 | None:intergenic |
| AACAGAGATTTACTCAATAT+AGG | 0.495924 | 4:-90693616 | None:intergenic |
| TGCGGTGGTTGAGGAGTTGG+TGG | 0.501694 | 4:-90693895 | None:intergenic |
| TTCAGGCATCTCAAACTATG+TGG | 0.519024 | 4:-90694061 | None:intergenic |
| GGACACGATCTCGTCTTACC+AGG | 0.528819 | 4:+90693937 | MsG0480023870.01.T01:CDS |
| GGTAAGACGAGATCGTGTCC+AGG | 0.534527 | 4:-90693934 | None:intergenic |
| ATTTACTCAATATAGGCCAT+TGG | 0.558368 | 4:-90693609 | None:intergenic |
| ATAGAACTGGTGGATACACT+AGG | 0.567688 | 4:+90693550 | MsG0480023870.01.T01:CDS |
| ATCAAGGGTGCTCTCTGCGG+TGG | 0.572040 | 4:-90693910 | None:intergenic |
| CTATCTACTACTATTACTGC+AGG | 0.573505 | 4:+90694135 | MsG0480023870.01.T01:three_prime_UTR |
| TTTGGGAGGAGCAAACTGCC+TGG | 0.576026 | 4:-90693955 | None:intergenic |
| AGTTGGTGATGCTGCACCAA+TGG | 0.577706 | 4:+90693593 | MsG0480023870.01.T01:CDS |
| GTGGATACACTAGGGTGCTA+CGG | 0.583066 | 4:+90693559 | MsG0480023870.01.T01:CDS |
| CTACGGACTCGCATTCGAGT+TGG | 0.599565 | 4:+90693576 | MsG0480023870.01.T01:CDS |
| TCAGGCATCTCAAACTATGT+GGG | 0.600864 | 4:-90694060 | None:intergenic |
| TACTATTACTGCAGGAACTG+AGG | 0.610274 | 4:+90694143 | MsG0480023870.01.T01:three_prime_UTR |
| GCAGTTTGCTCCTCCCAAAG+TGG | 0.612258 | 4:+90693959 | MsG0480023870.01.T01:CDS |
| AGATCGTGTCCAGGGATCAA+GGG | 0.612571 | 4:-90693925 | None:intergenic |
| TTGATCGCGATAATGAGCTT+AGG | 0.622821 | 4:+90693862 | MsG0480023870.01.T01:CDS |
| GTGTTGTGCAGAGATAGAAC+TGG | 0.623312 | 4:+90693537 | None:intergenic |
| GGGATCAAGGGTGCTCTCTG+CGG | 0.628911 | 4:-90693913 | None:intergenic |
| GTAAGACGAGATCGTGTCCA+GGG | 0.629636 | 4:-90693933 | None:intergenic |
| TTGTGCAGAGATAGAACTGG+TGG | 0.666108 | 4:+90693540 | MsG0480023870.01.T01:CDS |
| TAGAACTGGTGGATACACTA+GGG | 0.675074 | 4:+90693551 | MsG0480023870.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTATAAAATAAGAAAAATTC+AGG | - | Chr4:90694081-90694100 | None:intergenic | 10.0% |
| !!! | TTATTTTATAAATCTCCTCA+TGG | + | Chr4:90694090-90694109 | MsG0480023870.01.T01:three_prime_UTR | 20.0% |
| ! | AAATCTTAATATCCTAGTCT+AGG | - | Chr4:90693805-90693824 | None:intergenic | 25.0% |
| ! | AACAGAGATTTACTCAATAT+AGG | - | Chr4:90693619-90693638 | None:intergenic | 25.0% |
| !! | GTTTATCCTTCAAATCTTTT+AGG | + | Chr4:90693776-90693795 | MsG0480023870.01.T01:intron | 25.0% |
| !!! | AAAAAAATGTGCTTTGAAAC+AGG | + | Chr4:90693835-90693854 | MsG0480023870.01.T01:intron | 25.0% |
| ATTTACTCAATATAGGCCAT+TGG | - | Chr4:90693612-90693631 | None:intergenic | 30.0% | |
| CACACAATAGATTAAAAGAC+AGG | - | Chr4:90693747-90693766 | None:intergenic | 30.0% | |
| GTTAAACAGTGAAAATCAGA+AGG | - | Chr4:90693643-90693662 | None:intergenic | 30.0% | |
| ! | AGAATCAGATTTTTCCACTT+TGG | - | Chr4:90693976-90693995 | None:intergenic | 30.0% |
| ! | GAATCAGATTTTTCCACTTT+GGG | - | Chr4:90693975-90693994 | None:intergenic | 30.0% |
| ! | TTTTATTATTGCAAGCCATG+AGG | - | Chr4:90694108-90694127 | None:intergenic | 30.0% |
| ! | TTTTCACTGTTTAACTGACT+TGG | + | Chr4:90693648-90693667 | MsG0480023870.01.T01:intron | 30.0% |
| AGGCAACCTAAAAGATTTGA+AGG | - | Chr4:90693785-90693804 | None:intergenic | 35.0% | |
| CTATCTACTACTATTACTGC+AGG | + | Chr4:90694135-90694154 | MsG0480023870.01.T01:three_prime_UTR | 35.0% | |
| TTCTTTAGTGAACTCAAGCT+GGG | - | Chr4:90693695-90693714 | None:intergenic | 35.0% | |
| CTTCTTTAGTGAACTCAAGC+TGG | - | Chr4:90693696-90693715 | None:intergenic | 40.0% | |
| TACTATTACTGCAGGAACTG+AGG | + | Chr4:90694143-90694162 | MsG0480023870.01.T01:three_prime_UTR | 40.0% | |
| TCAGGCATCTCAAACTATGT+GGG | - | Chr4:90694063-90694082 | None:intergenic | 40.0% | |
| TTCAGGCATCTCAAACTATG+TGG | - | Chr4:90694064-90694083 | None:intergenic | 40.0% | |
| TTGATCGCGATAATGAGCTT+AGG | + | Chr4:90693862-90693881 | MsG0480023870.01.T01:CDS | 40.0% | |
| ! | ATAGAACTGGTGGATACACT+AGG | + | Chr4:90693550-90693569 | MsG0480023870.01.T01:CDS | 40.0% |
| ! | TAGAACTGGTGGATACACTA+GGG | + | Chr4:90693551-90693570 | MsG0480023870.01.T01:CDS | 40.0% |
| ! | TCAGATTTTTCCACTTTGGG+AGG | - | Chr4:90693972-90693991 | None:intergenic | 40.0% |
| !! | CACTGTTTAACTGACTTGGT+CGG | + | Chr4:90693652-90693671 | MsG0480023870.01.T01:intron | 40.0% |
| !! | CTTGGTCGGTTCTGTTATTT+TGG | + | Chr4:90693666-90693685 | MsG0480023870.01.T01:intron | 40.0% |
| !!! | TCTTTTAGGTTGCCTAGACT+AGG | + | Chr4:90693790-90693809 | MsG0480023870.01.T01:intron | 40.0% |
| TTGTGCAGAGATAGAACTGG+TGG | + | Chr4:90693540-90693559 | MsG0480023870.01.T01:CDS | 45.0% | |
| AGATCGTGTCCAGGGATCAA+GGG | - | Chr4:90693928-90693947 | None:intergenic | 50.0% | |
| GTAAGACGAGATCGTGTCCA+GGG | - | Chr4:90693936-90693955 | None:intergenic | 50.0% | |
| ! | GTGGATACACTAGGGTGCTA+CGG | + | Chr4:90693559-90693578 | MsG0480023870.01.T01:CDS | 50.0% |
| !! | AGTTGGTGATGCTGCACCAA+TGG | + | Chr4:90693593-90693612 | MsG0480023870.01.T01:CDS | 50.0% |
| CTACGGACTCGCATTCGAGT+TGG | + | Chr4:90693576-90693595 | MsG0480023870.01.T01:CDS | 55.0% | |
| GAGATCGTGTCCAGGGATCA+AGG | - | Chr4:90693929-90693948 | None:intergenic | 55.0% | |
| GGACACGATCTCGTCTTACC+AGG | + | Chr4:90693937-90693956 | MsG0480023870.01.T01:CDS | 55.0% | |
| GGTAAGACGAGATCGTGTCC+AGG | - | Chr4:90693937-90693956 | None:intergenic | 55.0% | |
| TTTGGGAGGAGCAAACTGCC+TGG | - | Chr4:90693958-90693977 | None:intergenic | 55.0% | |
| ! | GCAGTTTGCTCCTCCCAAAG+TGG | + | Chr4:90693959-90693978 | MsG0480023870.01.T01:CDS | 55.0% |
| CTCTGCGGTGGTTGAGGAGT+TGG | - | Chr4:90693901-90693920 | None:intergenic | 60.0% | |
| TGCGGTGGTTGAGGAGTTGG+TGG | - | Chr4:90693898-90693917 | None:intergenic | 60.0% | |
| !! | ATCAAGGGTGCTCTCTGCGG+TGG | - | Chr4:90693913-90693932 | None:intergenic | 60.0% |
| !! | CAGAGAGCACCCTTGATCCC+TGG | + | Chr4:90693916-90693935 | MsG0480023870.01.T01:CDS | 60.0% |
| !! | GGGATCAAGGGTGCTCTCTG+CGG | - | Chr4:90693916-90693935 | None:intergenic | 60.0% |
| GGTGCTCTCTGCGGTGGTTG+AGG | - | Chr4:90693907-90693926 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 90693540 | 90694187 | 90693540 | ID=MsG0480023870.01;Name=MsG0480023870.01 |
| Chr4 | mRNA | 90693540 | 90694187 | 90693540 | ID=MsG0480023870.01.T01;Parent=MsG0480023870.01;Name=MsG0480023870.01.T01;_AED=0.28;_eAED=0.28;_QI=0|1|0.5|1|1|1|2|174|80 |
| Chr4 | exon | 90693540 | 90693625 | 90693540 | ID=MsG0480023870.01.T01:exon:33816;Parent=MsG0480023870.01.T01 |
| Chr4 | exon | 90693857 | 90694187 | 90693857 | ID=MsG0480023870.01.T01:exon:33817;Parent=MsG0480023870.01.T01 |
| Chr4 | CDS | 90693540 | 90693625 | 90693540 | ID=MsG0480023870.01.T01:cds;Parent=MsG0480023870.01.T01 |
| Chr4 | CDS | 90693857 | 90694013 | 90693857 | ID=MsG0480023870.01.T01:cds;Parent=MsG0480023870.01.T01 |
| Chr4 | three_prime_UTR | 90694014 | 90694187 | 90694014 | ID=MsG0480023870.01.T01:three_prime_utr;Parent=MsG0480023870.01.T01 |
| Gene Sequence |
| Protein sequence |
