AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580024044.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580024044.01.T01 MTR_5g004870 85.882 170 15 2 2 166 114 279 3.54e-37 129
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 88 sgRNAs with CRISPR-Local

Find 100 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAGATATAAGAAACAAAATC+TGG 0.335243 5:-214319 None:intergenic
AAGAATTACTTACCAGCGTT+TGG 0.337334 5:-214123 None:intergenic
GTTTCTTATATCTCTACATT+AGG 0.348243 5:+214328 MsG0580024044.01.T01:three_prime_UTR
ATATGATGGGACTGCTGTAT+AGG 0.351177 5:-213764 None:intergenic
GGGTAAGGTGGTGGTGGTTG+TGG 0.356727 5:-213862 None:intergenic
GGCGGATAAGAGTGGGGAGG+AGG 0.387349 5:-214051 None:intergenic
GGACTGCTGTATAGGGAGTA+TGG 0.403544 5:-213756 None:intergenic
TATGATGGGACTGCTGTATA+GGG 0.411289 5:-213763 None:intergenic
GTAGCATATGTGTGTCAATA+TGG 0.415498 5:-214240 None:intergenic
GATGATGAGGGTGGTGGATA+GGG 0.427578 5:-213961 None:intergenic
ATTCACTTTGGCATTGGCAC+TGG 0.431726 5:-213609 None:intergenic
TTTGGCATTGGCACTGGCAT+TGG 0.438190 5:-213603 None:intergenic
CTGGTGATTCACTTTGGCAT+TGG 0.439416 5:-213615 None:intergenic
TGTGGGTAGTAGGAAGGAAC+AGG 0.447068 5:-213790 None:intergenic
GTGTGGTTGTTGTGGGTAGT+AGG 0.452219 5:-213800 None:intergenic
TGATGATGAGGGTGGTGGAT+AGG 0.463785 5:-213962 None:intergenic
GATGACACTGATTGAGGGTA+TGG 0.470349 5:-213937 None:intergenic
AATTAGAGTGTGGTTGTTGT+GGG 0.481190 5:-213807 None:intergenic
GATGATGAGGTTGGTGGATA+GGG 0.488772 5:-214012 None:intergenic
CTGAAGATGGCGGATAAGAG+TGG 0.491172 5:-214059 None:intergenic
ACAAAATCACGGTGGTTCCT+TGG 0.491656 5:-214271 None:intergenic
TAATTAGAGTGTGGTTGTTG+TGG 0.492816 5:-213808 None:intergenic
TATGGATATGTTGAGGATGG+TGG 0.494303 5:-213919 None:intergenic
CCTGCAGGTGGAGGGTATGA+AGG 0.495024 5:-214093 None:intergenic
GAAGGAACAGGATTATATGA+TGG 0.495410 5:-213778 None:intergenic
TGATGATGAGGTTGGTGGAT+AGG 0.496782 5:-214013 None:intergenic
TGGGGAGGAGGGTACGGTGA+TGG 0.496971 5:-214039 None:intergenic
TAAGAGTGGGGAGGAGGGTA+CGG 0.498100 5:-214045 None:intergenic
GGAGTATGGCTTGAGGAGCC+TGG 0.500219 5:-213742 None:intergenic
TAAGCAACAGCAGGTGTTGT+TGG 0.507242 5:-213838 None:intergenic
TAGGGAGATGACACTGATTG+AGG 0.507262 5:-213943 None:intergenic
TAGGGAGATGGCACTGAAGG+AGG 0.510794 5:-213994 None:intergenic
GATGGCACTGAAGGAGGGTA+TGG 0.515857 5:-213988 None:intergenic
GGTTACCTGGTGATTCACTT+TGG 0.517974 5:-213621 None:intergenic
GGAGGATAACCTGCAGGTGG+AGG 0.519441 5:-214102 None:intergenic
GTGTGTCAATATGGCGGTGG+AGG 0.520887 5:-214231 None:intergenic
CCTTCATACCCTCCACCTGC+AGG 0.529204 5:+214093 MsG0580024044.01.T01:CDS
AATTACTTACCAGCGTTTGG+AGG 0.530399 5:-214120 None:intergenic
AGGGAGATGACACTGATTGA+GGG 0.533173 5:-213942 None:intergenic
ATAACGAAGATGTGGATTAG+TGG 0.533513 5:+214456 MsG0580024044.01.T01:three_prime_UTR
GGATAGGGAGATGGCACTGA+AGG 0.535955 5:-213997 None:intergenic
GGTGGAGGGTATGAAGGTGG+TGG 0.549199 5:-214087 None:intergenic
GGTGATGATGATGAGGGTGG+TGG 0.549858 5:-213967 None:intergenic
TGAAGATGGCGGATAAGAGT+GGG 0.557216 5:-214058 None:intergenic
GCAGCAGGGTAAGGTGGTGG+TGG 0.559747 5:-213868 None:intergenic
GAGGATGGTGGATAAGAAGA+TGG 0.563718 5:-213907 None:intergenic
TTATGTGAATGTGAAGCAGC+AGG 0.565883 5:-213883 None:intergenic
AAGGAACAGGATTATATGAT+GGG 0.567570 5:-213777 None:intergenic
AGGGTATGGTGATGATGATG+AGG 0.569188 5:-213974 None:intergenic
GAATGTGAAGCAGCAGGGTA+AGG 0.573678 5:-213877 None:intergenic
GGTGATGATGATGAGGTTGG+TGG 0.578742 5:-214018 None:intergenic
AGATATAAGAAACAAAATCT+GGG 0.579766 5:-214318 None:intergenic
GGTTGTTGTGGGTAGTAGGA+AGG 0.584669 5:-213796 None:intergenic
ATTCTATTATAACGAAGATG+TGG 0.584978 5:+214448 MsG0580024044.01.T01:three_prime_UTR
AGGGAGATGGCACTGAAGGA+GGG 0.585482 5:-213993 None:intergenic
GATGGTGATGATGATGAGGT+TGG 0.587170 5:-214021 None:intergenic
GGGTATGGATATGTTGAGGA+TGG 0.588607 5:-213922 None:intergenic
GCGTTTGGAGGATAACCTGC+AGG 0.588898 5:-214108 None:intergenic
GAGGATAACCTGCAGGTGGA+GGG 0.591147 5:-214101 None:intergenic
GCGGATAAGAGTGGGGAGGA+GGG 0.591531 5:-214050 None:intergenic
CGGTGATGGTGATGATGATG+AGG 0.592680 5:-214025 None:intergenic
GAGGTTGGTGGATAGGGAGA+TGG 0.593101 5:-214006 None:intergenic
CAACAGCAGGTGTTGTTGGA+TGG 0.604074 5:-213834 None:intergenic
CAATGCCAAAGTGAATCACC+AGG 0.609446 5:+213616 MsG0580024044.01.T01:CDS
ATATGCTACTTTCGCTACCA+AGG 0.613661 5:+214254 MsG0580024044.01.T01:three_prime_UTR
ACTAATCATGTACAAAATCA+CGG 0.618703 5:-214282 None:intergenic
GATGGCGGATAAGAGTGGGG+AGG 0.623756 5:-214054 None:intergenic
GAAGCAGCAGGGTAAGGTGG+TGG 0.624794 5:-213871 None:intergenic
GCTAATATATTGCAGAAACC+AGG 0.627769 5:+213724 MsG0580024044.01.T01:intron
GGTGGTGGATAAGCTGAAGA+TGG 0.630978 5:-214072 None:intergenic
GCAGGTGGAGGGTATGAAGG+TGG 0.632925 5:-214090 None:intergenic
GGGTATGGTGATGATGATGA+GGG 0.634043 5:-213973 None:intergenic
GGTTGTGGATAAGCAACAGC+AGG 0.634892 5:-213847 None:intergenic
GGATGGTGATAATTAGAGTG+TGG 0.637190 5:-213817 None:intergenic
TATGGTGATGATGATGAGGG+TGG 0.640824 5:-213970 None:intergenic
TGTGTCAATATGGCGGTGGA+GGG 0.643568 5:-214230 None:intergenic
TATGTGAATGTGAAGCAGCA+GGG 0.647936 5:-213882 None:intergenic
GCAGGTTATCCTCCAAACGC+TGG 0.651932 5:+214111 MsG0580024044.01.T01:CDS
TGTGAAGCAGCAGGGTAAGG+TGG 0.654828 5:-213874 None:intergenic
TTTGGAGGATAACCTGCAGG+TGG 0.658511 5:-214105 None:intergenic
GGTGGATAAGCTGAAGATGG+CGG 0.670105 5:-214069 None:intergenic
TATGTGTGTCAATATGGCGG+TGG 0.670842 5:-214234 None:intergenic
TTGAGGGTATGGATATGTTG+AGG 0.674935 5:-213926 None:intergenic
TGTATAGGGAGTATGGCTTG+AGG 0.692075 5:-213749 None:intergenic
TGTCTGTCTGACAGACACGC+AGG 0.700944 5:+213554 None:intergenic
GAAGATGGCGGATAAGAGTG+GGG 0.705455 5:-214057 None:intergenic
GCATATGTGTGTCAATATGG+CGG 0.756030 5:-214237 None:intergenic
AATCATGTACAAAATCACGG+TGG 0.820568 5:-214279 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TACATATATATAATAACAAT+TGG + Chr5:213672-213691 MsG0580024044.01.T01:intron 10.0%
!! ATAAATAGTAATGAAAATGT+AGG - Chr5:213645-213664 None:intergenic 15.0%
!!! ACTATCTATCATTATTTTTT+TGG + Chr5:214412-214431 MsG0580024044.01.T01:three_prime_UTR 15.0%
!!! CTATCTATCATTATTTTTTT+GGG + Chr5:214413-214432 MsG0580024044.01.T01:three_prime_UTR 15.0%
!! AGATATAAGAAACAAAATCT+GGG - Chr5:214321-214340 None:intergenic 20.0%
! ACTAATCATGTACAAAATCA+CGG - Chr5:214285-214304 None:intergenic 25.0%
! AGTAGATGTGAAAATTGAAA+TGG - Chr5:214162-214181 None:intergenic 25.0%
! ATTCTATTATAACGAAGATG+TGG + Chr5:214448-214467 MsG0580024044.01.T01:three_prime_UTR 25.0%
! GAGATATAAGAAACAAAATC+TGG - Chr5:214322-214341 None:intergenic 25.0%
! GATTCTTAATAAGTGTGTTT+GGG + Chr5:213699-213718 MsG0580024044.01.T01:intron 25.0%
! GTTTCTTATATCTCTACATT+AGG + Chr5:214328-214347 MsG0580024044.01.T01:three_prime_UTR 25.0%
! TGATTCTTAATAAGTGTGTT+TGG + Chr5:213698-213717 MsG0580024044.01.T01:intron 25.0%
AAGGAACAGGATTATATGAT+GGG - Chr5:213780-213799 None:intergenic 30.0%
GATGTGAAAATTGAAATGGT+TGG - Chr5:214158-214177 None:intergenic 30.0%
TAATGAAAATGTAGGTTACC+TGG - Chr5:213637-213656 None:intergenic 30.0%
AAGAATTACTTACCAGCGTT+TGG - Chr5:214126-214145 None:intergenic 35.0%
AATCATGTACAAAATCACGG+TGG - Chr5:214282-214301 None:intergenic 35.0%
AATTAGAGTGTGGTTGTTGT+GGG - Chr5:213810-213829 None:intergenic 35.0%
ATCCTGAAAAAGGATGTGAT+TGG - Chr5:214206-214225 None:intergenic 35.0%
GAAAAAGGATGTGATTGGAA+AGG - Chr5:214201-214220 None:intergenic 35.0%
GAAGGAACAGGATTATATGA+TGG - Chr5:213781-213800 None:intergenic 35.0%
GCTAATATATTGCAGAAACC+AGG + Chr5:213724-213743 MsG0580024044.01.T01:intron 35.0%
GTAGCATATGTGTGTCAATA+TGG - Chr5:214243-214262 None:intergenic 35.0%
TAATTAGAGTGTGGTTGTTG+TGG - Chr5:213811-213830 None:intergenic 35.0%
! TTCCAATCACATCCTTTTTC+AGG + Chr5:214201-214220 MsG0580024044.01.T01:intron 35.0%
!! ATAACGAAGATGTGGATTAG+TGG + Chr5:214456-214475 MsG0580024044.01.T01:three_prime_UTR 35.0%
AATTACTTACCAGCGTTTGG+AGG - Chr5:214123-214142 None:intergenic 40.0%
GCATATGTGTGTCAATATGG+CGG - Chr5:214240-214259 None:intergenic 40.0%
GGATGGTGATAATTAGAGTG+TGG - Chr5:213820-213839 None:intergenic 40.0%
TATGGATATGTTGAGGATGG+TGG - Chr5:213922-213941 None:intergenic 40.0%
TATGTGAATGTGAAGCAGCA+GGG - Chr5:213885-213904 None:intergenic 40.0%
TTATGTGAATGTGAAGCAGC+AGG - Chr5:213886-213905 None:intergenic 40.0%
! ATATGATGGGACTGCTGTAT+AGG - Chr5:213767-213786 None:intergenic 40.0%
! TATGATGGGACTGCTGTATA+GGG - Chr5:213766-213785 None:intergenic 40.0%
! TTGAGGGTATGGATATGTTG+AGG - Chr5:213929-213948 None:intergenic 40.0%
!! ATATGCTACTTTCGCTACCA+AGG + Chr5:214254-214273 MsG0580024044.01.T01:three_prime_UTR 40.0%
ACAAAATCACGGTGGTTCCT+TGG - Chr5:214274-214293 None:intergenic 45.0%
CAATGCCAAAGTGAATCACC+AGG + Chr5:213616-213635 MsG0580024044.01.T01:CDS 45.0%
CTGGTGATTCACTTTGGCAT+TGG - Chr5:213618-213637 None:intergenic 45.0%
GGAGGGTAGAATCCTGAAAA+AGG - Chr5:214216-214235 None:intergenic 45.0%
GGGTATGGATATGTTGAGGA+TGG - Chr5:213925-213944 None:intergenic 45.0%
GGTTACCTGGTGATTCACTT+TGG - Chr5:213624-213643 None:intergenic 45.0%
TATGTGTGTCAATATGGCGG+TGG - Chr5:214237-214256 None:intergenic 45.0%
! AGGGAGATGACACTGATTGA+GGG - Chr5:213945-213964 None:intergenic 45.0%
! AGGGTATGGTGATGATGATG+AGG - Chr5:213977-213996 None:intergenic 45.0%
! GATGACACTGATTGAGGGTA+TGG - Chr5:213940-213959 None:intergenic 45.0%
! GATGGTGATGATGATGAGGT+TGG - Chr5:214024-214043 None:intergenic 45.0%
! GGGTATGGTGATGATGATGA+GGG - Chr5:213976-213995 None:intergenic 45.0%
! TAAGCAACAGCAGGTGTTGT+TGG - Chr5:213841-213860 None:intergenic 45.0%
! TAGGGAGATGACACTGATTG+AGG - Chr5:213946-213965 None:intergenic 45.0%
! TATGGTGATGATGATGAGGG+TGG - Chr5:213973-213992 None:intergenic 45.0%
! TGTATAGGGAGTATGGCTTG+AGG - Chr5:213752-213771 None:intergenic 45.0%
!! ATTCACTTTGGCATTGGCAC+TGG - Chr5:213612-213631 None:intergenic 45.0%
!! GAGGATGGTGGATAAGAAGA+TGG - Chr5:213910-213929 None:intergenic 45.0%
!! GATGATGAGGTTGGTGGATA+GGG - Chr5:214015-214034 None:intergenic 45.0%
!! TGAAGATGGCGGATAAGAGT+GGG - Chr5:214061-214080 None:intergenic 45.0%
!! TGATGATGAGGTTGGTGGAT+AGG - Chr5:214016-214035 None:intergenic 45.0%
GAATGTGAAGCAGCAGGGTA+AGG - Chr5:213880-213899 None:intergenic 50.0%
GGACTGCTGTATAGGGAGTA+TGG - Chr5:213759-213778 None:intergenic 50.0%
GGTGGATAAGCTGAAGATGG+CGG - Chr5:214072-214091 None:intergenic 50.0%
GGTTGTGGATAAGCAACAGC+AGG - Chr5:213850-213869 None:intergenic 50.0%
GGTTGTTGTGGGTAGTAGGA+AGG - Chr5:213799-213818 None:intergenic 50.0%
GTGTGGTTGTTGTGGGTAGT+AGG - Chr5:213803-213822 None:intergenic 50.0%
TGTGGGTAGTAGGAAGGAAC+AGG - Chr5:213793-213812 None:intergenic 50.0%
TGTGTCAATATGGCGGTGGA+GGG - Chr5:214233-214252 None:intergenic 50.0%
TTTGGAGGATAACCTGCAGG+TGG - Chr5:214108-214127 None:intergenic 50.0%
! CGGTGATGGTGATGATGATG+AGG - Chr5:214028-214047 None:intergenic 50.0%
! GGTGATGATGATGAGGTTGG+TGG - Chr5:214021-214040 None:intergenic 50.0%
!! CAACAGCAGGTGTTGTTGGA+TGG - Chr5:213837-213856 None:intergenic 50.0%
!! CTGAAGATGGCGGATAAGAG+TGG - Chr5:214062-214081 None:intergenic 50.0%
!! GAAGATGGCGGATAAGAGTG+GGG - Chr5:214060-214079 None:intergenic 50.0%
!! GATGATGAGGGTGGTGGATA+GGG - Chr5:213964-213983 None:intergenic 50.0%
!! GGTGGTGGATAAGCTGAAGA+TGG - Chr5:214075-214094 None:intergenic 50.0%
!! TGATGATGAGGGTGGTGGAT+AGG - Chr5:213965-213984 None:intergenic 50.0%
!! TTTGGCATTGGCACTGGCAT+TGG - Chr5:213606-213625 None:intergenic 50.0%
GAGGATAACCTGCAGGTGGA+GGG - Chr5:214104-214123 None:intergenic 55.0%
GCAGGTTATCCTCCAAACGC+TGG + Chr5:214111-214130 MsG0580024044.01.T01:CDS 55.0%
GCGTTTGGAGGATAACCTGC+AGG - Chr5:214111-214130 None:intergenic 55.0%
GTGTGTCAATATGGCGGTGG+AGG - Chr5:214234-214253 None:intergenic 55.0%
TAAGAGTGGGGAGGAGGGTA+CGG - Chr5:214048-214067 None:intergenic 55.0%
TGTGAAGCAGCAGGGTAAGG+TGG - Chr5:213877-213896 None:intergenic 55.0%
! AGGGAGATGGCACTGAAGGA+GGG - Chr5:213996-214015 None:intergenic 55.0%
! GATGGCACTGAAGGAGGGTA+TGG - Chr5:213991-214010 None:intergenic 55.0%
! GGATAGGGAGATGGCACTGA+AGG - Chr5:214000-214019 None:intergenic 55.0%
! GGTGATGATGATGAGGGTGG+TGG - Chr5:213970-213989 None:intergenic 55.0%
! TAGGGAGATGGCACTGAAGG+AGG - Chr5:213997-214016 None:intergenic 55.0%
!! GAGGTTGGTGGATAGGGAGA+TGG - Chr5:214009-214028 None:intergenic 55.0%
CCTTCATACCCTCCACCTGC+AGG + Chr5:214093-214112 MsG0580024044.01.T01:CDS 60.0%
GAAGCAGCAGGGTAAGGTGG+TGG - Chr5:213874-213893 None:intergenic 60.0%
GCGGATAAGAGTGGGGAGGA+GGG - Chr5:214053-214072 None:intergenic 60.0%
GGAGGATAACCTGCAGGTGG+AGG - Chr5:214105-214124 None:intergenic 60.0%
GGAGTATGGCTTGAGGAGCC+TGG - Chr5:213745-213764 None:intergenic 60.0%
! CCTGCAGGTGGAGGGTATGA+AGG - Chr5:214096-214115 None:intergenic 60.0%
! GCAGGTGGAGGGTATGAAGG+TGG - Chr5:214093-214112 None:intergenic 60.0%
! GGTGGAGGGTATGAAGGTGG+TGG - Chr5:214090-214109 None:intergenic 60.0%
!! GATGGCGGATAAGAGTGGGG+AGG - Chr5:214057-214076 None:intergenic 60.0%
!! GGGTAAGGTGGTGGTGGTTG+TGG - Chr5:213865-213884 None:intergenic 60.0%
GGCGGATAAGAGTGGGGAGG+AGG - Chr5:214054-214073 None:intergenic 65.0%
! GCAGCAGGGTAAGGTGGTGG+TGG - Chr5:213871-213890 None:intergenic 65.0%
! TGGGGAGGAGGGTACGGTGA+TGG - Chr5:214042-214061 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
Chr5 gene 213557 214528 213557 ID=MsG0580024044.01;Name=MsG0580024044.01
Chr5 mRNA 213557 214528 213557 ID=MsG0580024044.01.T01;Parent=MsG0580024044.01;Name=MsG0580024044.01.T01;_AED=0.33;_eAED=0.34;_QI=0|1|0.66|1|0|0|3|280|166
Chr5 exon 213557 213637 213557 ID=MsG0580024044.01.T01:exon:22989;Parent=MsG0580024044.01.T01
Chr5 exon 213739 214132 213739 ID=MsG0580024044.01.T01:exon:22990;Parent=MsG0580024044.01.T01
Chr5 exon 214223 214528 214223 ID=MsG0580024044.01.T01:exon:22991;Parent=MsG0580024044.01.T01
Chr5 CDS 213557 213637 213557 ID=MsG0580024044.01.T01:cds;Parent=MsG0580024044.01.T01
Chr5 CDS 213739 214132 213739 ID=MsG0580024044.01.T01:cds;Parent=MsG0580024044.01.T01
Chr5 CDS 214223 214248 214223 ID=MsG0580024044.01.T01:cds;Parent=MsG0580024044.01.T01
Chr5 three_prime_UTR 214249 214528 214249 ID=MsG0580024044.01.T01:three_prime_utr;Parent=MsG0580024044.01.T01
Gene Sequence

>MsG0580024044.01.T01

CTGTCTGACAGACACGCAGGCGAAGTAAAACTCATACTGCATTATGCCAATGCCAGTGCCAATGCCAAAGTGAATCACCAGAAACCAGGCTCCTCAAGCCATACTCCCTATACAGCAGTCCCATCATATAATCCTGTTCCTTCCTACTACCCACAACAACCACACTCTAATTATCACCATCCAACAACACCTGCTGTTGCTTATCCACAACCACCACCACCTTACCCTGCTGCTTCACATTCACATAATCCATCTTCTTATCCACCATCCTCAACATATCCATACCCTCAATCAGTGTCATCTCCCTATCCACCACCCTCATCATCATCACCATACCCTCCTTCAGTGCCATCTCCCTATCCACCAACCTCATCATCATCACCATCACCGTACCCTCCTCCCCACTCTTATCCGCCATCTTCAGCTTATCCACCACCTTCATACCCTCCACCTGCAGGTTATCCTCCAAACGCTGGATTCTACCCTCCACCGCCATATTGA

Protein sequence

>MsG0580024044.01.T01

LSDRHAGEVKLILHYANASANAKVNHQKPGSSSHTPYTAVPSYNPVPSYYPQQPHSNYHHPTTPAVAYPQPPPPYPAASHSHNPSSYPPSSTYPYPQSVSSPYPPPSSSSPYPPSVPSPYPPTSSSSPSPYPPPHSYPPSSAYPPPSYPPPAGYPPNAGFYPPPPY*