AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580024343.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580024343.01.T01 MTR_5g009310 90.323 186 7 1 1 175 1 186 1.42e-116 328
MsG0580024343.01.T01 MTR_8g099455 70.968 186 43 1 1 175 1 186 4.09e-89 259
MsG0580024343.01.T01 MTR_8g099455 70.968 186 43 1 1 175 62 247 6.33e-88 258
MsG0580024343.01.T01 MTR_5g009310 97.959 98 2 0 1 98 1 98 2.80e-64 194
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580024343.01.T01 AT4G23895 66.129 186 50 2 1 175 60 243 2.83e-79 236
MsG0580024343.01.T01 AT4G23895 66.129 186 50 2 1 175 60 243 2.83e-79 236
MsG0580024343.01.T01 AT4G23895 66.129 186 50 2 1 175 60 243 2.83e-79 236
MsG0580024343.01.T01 AT4G23895 68.182 176 43 2 1 165 60 233 4.70e-75 232

Find 67 sgRNAs with CRISPR-Local

Find 197 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCGCACAATCTAGCTTATTT+TGG 0.119039 5:+3599568 MsG0580024343.01.T01:five_prime_UTR
CACGCTTAATACTTGGTAAT+TGG 0.189394 5:-3601018 None:intergenic
TCCTCGCTTTGTTGTTCTTC+AGG 0.207772 5:+3600558 MsG0580024343.01.T01:CDS
AATAGAGACTGATGATTATT+TGG 0.240870 5:+3601478 MsG0580024343.01.T01:three_prime_UTR
TGAGTTCCAGATTCAGTAAT+AGG 0.270097 5:-3599479 None:intergenic
ATCAGAACTTGTTGATTTAG+AGG 0.291898 5:+3599823 MsG0580024343.01.T01:CDS
CCTCGCTTTGTTGTTCTTCA+GGG 0.314121 5:+3600559 MsG0580024343.01.T01:CDS
GGCATTTCCTTGAGAACTTA+AGG 0.331835 5:+3599397 MsG0580024343.01.T01:five_prime_UTR
AAGGCACCTATTACTGAATC+TGG 0.344705 5:+3599473 MsG0580024343.01.T01:five_prime_UTR
GATGTTTCAGGATTATTTAT+CGG 0.365961 5:+3599338 MsG0580024343.01.T01:intron
CCATCTTCACGCTTAATACT+TGG 0.382946 5:-3601025 None:intergenic
TTGCGGTCAGCTCGTCTATC+CGG 0.396043 5:+3600354 MsG0580024343.01.T01:CDS
TTGAAATTGTTTACACTTCG+AGG 0.404814 5:+3599640 MsG0580024343.01.T01:CDS
CAGAGATGGACAGAGCTTCC+AGG 0.406603 5:+3600493 MsG0580024343.01.T01:intron
CTCGAGCAGTTCTAAGATAC+AGG 0.424024 5:+3601102 MsG0580024343.01.T01:CDS
TCTGCAATCGGACTGCAAAC+TGG 0.436293 5:+3601299 MsG0580024343.01.T01:CDS
TCTACATCGGTATCATCAAT+AGG 0.447712 5:-3600526 None:intergenic
ACCTATCCAGAGGAAGATAA+TGG 0.451212 5:-3599373 None:intergenic
TTATCGCCATTATCTTCCTC+TGG 0.456512 5:+3599367 MsG0580024343.01.T01:five_prime_UTR
ACTTGTTGATTTAGAGGAGA+AGG 0.457112 5:+3599829 MsG0580024343.01.T01:CDS
TTAGTTAGTTGTACCTGGCC+TGG 0.458537 5:-3598540 None:intergenic
AATGGATTTCTTTCATTCGA+AGG 0.473694 5:-3598457 None:intergenic
TTATCTTCCTCTGGATAGGT+TGG 0.480663 5:+3599376 MsG0580024343.01.T01:five_prime_UTR
CTTCTCTTCTTAACACGGCC+AGG 0.481417 5:+3598522 MsG0580024343.01.T01:five_prime_UTR
ATGTCGTGTTAAAATACAAA+AGG 0.492182 5:-3601062 None:intergenic
GGTTCGGATGTATAAATATC+CGG 0.494584 5:-3600373 None:intergenic
CTTGGTGTTCTTCAAGTCCT+CGG 0.496975 5:-3599604 None:intergenic
CCAAGTATTAAGCGTGAAGA+TGG 0.499854 5:+3601025 MsG0580024343.01.T01:CDS
AAGTAGGTAGATACTACTAA+TGG 0.500409 5:-3598475 None:intergenic
CTTCTTAACACGGCCAGGCC+AGG 0.500500 5:+3598527 MsG0580024343.01.T01:five_prime_UTR
TTACATCTATATCGAACCAT+TGG 0.502885 5:-3601340 None:intergenic
ATGATACCGATGTAGATGAT+TGG 0.507290 5:+3600533 MsG0580024343.01.T01:CDS
GAGCCCTAGTAGTGGTAGTC+GGG 0.510554 5:+3599672 MsG0580024343.01.T01:CDS
CTTCTCTTCTCTTCTTAACA+CGG 0.512426 5:+3598517 MsG0580024343.01.T01:five_prime_UTR
TAGTAGTGGTAGTCGGGAAA+AGG 0.515903 5:+3599678 MsG0580024343.01.T01:CDS
TTCAACATGAAAGCAGCCCT+TGG 0.520437 5:-3599622 None:intergenic
GTGTTCTTCAAGTCCTCGGA+TGG 0.524855 5:-3599600 None:intergenic
GTGGTTATCAGCTCTGCAAT+CGG 0.533434 5:+3601287 MsG0580024343.01.T01:CDS
TACATCTATATCGAACCATT+GGG 0.534421 5:-3601339 None:intergenic
TTACACTTCGAGGGCTGCCA+TGG 0.535812 5:+3599650 MsG0580024343.01.T01:CDS
CAGATGACCTTAAGTTCTCA+AGG 0.536063 5:-3599404 None:intergenic
GGACGAAAACGAAGGTAAGT+AGG 0.543421 5:-3598491 None:intergenic
TCAATAGGTGGAGATTCTCC+TGG 0.549524 5:-3600511 None:intergenic
TTGGGCCTTGGTGCCATCCG+AGG 0.549919 5:+3599587 MsG0580024343.01.T01:five_prime_UTR
TGAAATTGTTTACACTTCGA+GGG 0.554877 5:+3599641 MsG0580024343.01.T01:CDS
TGTACAAGAAATAATAGTCA+TGG 0.573002 5:-3601437 None:intergenic
GCCATTATCTTCCTCTGGAT+AGG 0.579320 5:+3599372 MsG0580024343.01.T01:five_prime_UTR
GGAGCCCTAGTAGTGGTAGT+CGG 0.588289 5:+3599671 MsG0580024343.01.T01:CDS
TGTTGATTTAGAGGAGAAGG+AGG 0.603598 5:+3599832 MsG0580024343.01.T01:CDS
CTACCACTACTAGGGCTCCA+TGG 0.603848 5:-3599667 None:intergenic
AATAAAGATCAACACTCTGA+AGG 0.618147 5:+3599538 MsG0580024343.01.T01:five_prime_UTR
TTTCCCGACTACCACTACTA+GGG 0.626466 5:-3599675 None:intergenic
TCTGATGCATCAACTCCCAA+TGG 0.627776 5:+3601324 MsG0580024343.01.T01:CDS
CCCTGAAGAACAACAAAGCG+AGG 0.633323 5:-3600559 None:intergenic
TGTAGAATATCTTGAGAACA+AGG 0.639246 5:+3599454 MsG0580024343.01.T01:five_prime_UTR
AGAAGAGAGGACGAAAACGA+AGG 0.639661 5:-3598499 None:intergenic
CTGCCATGGAGCCCTAGTAG+TGG 0.643912 5:+3599664 MsG0580024343.01.T01:CDS
AAAGCTAAACAAAAGACTAG+AGG 0.644343 5:+3598404 None:intergenic
TTAAGAAGAGAAGAGAAGAG+AGG 0.655292 5:-3598512 None:intergenic
GATAGAAGTGTTGAGTCGTG+AGG 0.659394 5:-3600977 None:intergenic
AGGAAGCCAATCATCTACAT+CGG 0.663664 5:-3600539 None:intergenic
CAAGTCCTCGGATGGCACCA+AGG 0.664072 5:-3599592 None:intergenic
ACATCGGTATCATCAATAGG+TGG 0.664944 5:-3600523 None:intergenic
GAGGACTTGAAGAACACCAA+GGG 0.673825 5:+3599606 MsG0580024343.01.T01:five_prime_UTR
GGAAATGCCAACCTATCCAG+AGG 0.683785 5:-3599383 None:intergenic
GGCGTGTCGTACTCGTACGT+AGG 0.700221 5:+3598425 MsG0580024343.01.T01:five_prime_UTR
CGAGGACTTGAAGAACACCA+AGG 0.708009 5:+3599605 MsG0580024343.01.T01:five_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAAAAAGGTTAAATTTGT+AGG - Chr5:3598628-3598647 None:intergenic 15.0%
!! TATGCAATATGATATTTAAT+TGG + Chr5:3598823-3598842 MsG0580024343.01.T01:intron 15.0%
!!! AAAATATTGATGAAATTTTG+CGG + Chr5:3600337-3600356 MsG0580024343.01.T01:CDS 15.0%
!!! TACTTTTCTTCTTTAAATTA+CGG - Chr5:3599134-3599153 None:intergenic 15.0%
!! AAAACAAAAGTAATAAAGAG+AGG - Chr5:3598594-3598613 None:intergenic 20.0%
!! AAAATAAACTAATGAAGGTT+TGG - Chr5:3600411-3600430 None:intergenic 20.0%
!! AAATAAACTAATGAAGGTTT+GGG - Chr5:3600410-3600429 None:intergenic 20.0%
!! ATAAACTGAATATGTAGAAT+CGG - Chr5:3599072-3599091 None:intergenic 20.0%
!! ATAAGGATAAAAAAATAGAG+AGG - Chr5:3600798-3600817 None:intergenic 20.0%
!! TAATCAGATATTCTTAAGTT+AGG - Chr5:3601132-3601151 None:intergenic 20.0%
!!! AAGTTGATTAATGAAGTTTA+GGG + Chr5:3598689-3598708 MsG0580024343.01.T01:intron 20.0%
!!! CAAAGTCAATTATATTTTGT+AGG + Chr5:3599766-3599785 MsG0580024343.01.T01:intron 20.0%
!!! CTACAAAATATAATTGACTT+TGG - Chr5:3599768-3599787 None:intergenic 20.0%
!!! CTCTTTTTACCAATATTTTT+CGG + Chr5:3600900-3600919 MsG0580024343.01.T01:intron 20.0%
!!! TAAGTTGATTAATGAAGTTT+AGG + Chr5:3598688-3598707 MsG0580024343.01.T01:intron 20.0%
!!! TTGATTTTTTTATTTGACAC+AGG + Chr5:3600310-3600329 MsG0580024343.01.T01:intron 20.0%
!!! TTTAACCTTTTTTATTCTCT+TGG + Chr5:3598633-3598652 MsG0580024343.01.T01:intron 20.0%
! AATAGAGACTGATGATTATT+TGG + Chr5:3601478-3601497 MsG0580024343.01.T01:three_prime_UTR 25.0%
! AATATGGTAGTACTTAGATA+CGG + Chr5:3599243-3599262 MsG0580024343.01.T01:intron 25.0%
! ACAATCCAAGAGAATAAAAA+AGG - Chr5:3598641-3598660 None:intergenic 25.0%
! AGAATATAAGAGAGATTCAT+CGG - Chr5:3600677-3600696 None:intergenic 25.0%
! AGCAAAAAAGAATCACTATA+TGG - Chr5:3600186-3600205 None:intergenic 25.0%
! AGTTGAGAATCAGAATATTT+AGG + Chr5:3598760-3598779 MsG0580024343.01.T01:intron 25.0%
! ATGTCGTGTTAAAATACAAA+AGG - Chr5:3601065-3601084 None:intergenic 25.0%
! CGAAAAATATTGGTAAAAAG+AGG - Chr5:3600902-3600921 None:intergenic 25.0%
! GATGCAAAATAAACTAATGA+AGG - Chr5:3600416-3600435 None:intergenic 25.0%
! GATGTTTCAGGATTATTTAT+CGG + Chr5:3599338-3599357 MsG0580024343.01.T01:intron 25.0%
! TAGTTTAAAAGCAAGTTTGT+CGG + Chr5:3598969-3598988 MsG0580024343.01.T01:intron 25.0%
! TGAAGAACTACAAAACAATA+AGG - Chr5:3600156-3600175 None:intergenic 25.0%
! TGTACAAGAAATAATAGTCA+TGG - Chr5:3601440-3601459 None:intergenic 25.0%
!! AAAGTCCAAAAATCCTTTTA+GGG - Chr5:3599201-3599220 None:intergenic 25.0%
!! ATAGTGGAACAGTTATTAAT+TGG - Chr5:3598861-3598880 None:intergenic 25.0%
!! GACTTTATATTTTGCAAGAT+TGG + Chr5:3599215-3599234 MsG0580024343.01.T01:intron 25.0%
!! TAAAGTCCAAAAATCCTTTT+AGG - Chr5:3599202-3599221 None:intergenic 25.0%
!! TTCATATATTTGTTGAGTAC+AGG + Chr5:3600592-3600611 MsG0580024343.01.T01:intron 25.0%
!! TTTTATTCTCTTGGATTGTT+TGG + Chr5:3598642-3598661 MsG0580024343.01.T01:intron 25.0%
!!! ATTCTTTTTTGCTGTTACAA+AGG + Chr5:3600193-3600212 MsG0580024343.01.T01:intron 25.0%
!!! TCATTTTTTATCTACAGAGA+TGG + Chr5:3600479-3600498 MsG0580024343.01.T01:intron 25.0%
!!! TCTTTTATTCCTTTTTAAGG+TGG + Chr5:3601260-3601279 MsG0580024343.01.T01:intron 25.0%
!!! TTTTGTATTTTAACACGACA+TGG + Chr5:3601064-3601083 MsG0580024343.01.T01:CDS 25.0%
!!! TTTTTTTATTTGACACAGGT+TGG + Chr5:3600314-3600333 MsG0580024343.01.T01:intron 25.0%
AAGTAGGTAGATACTACTAA+TGG - Chr5:3598478-3598497 None:intergenic 30.0%
AAGTGATTGTCTTAGTAGTA+GGG + Chr5:3600111-3600130 MsG0580024343.01.T01:intron 30.0%
AATAAAGATCAACACTCTGA+AGG + Chr5:3599538-3599557 MsG0580024343.01.T01:five_prime_UTR 30.0%
AATAGAGAGGAGAGAAAAAA+AGG - Chr5:3600785-3600804 None:intergenic 30.0%
AGAATCAGAATATTTAGGTG+GGG + Chr5:3598765-3598784 MsG0580024343.01.T01:intron 30.0%
AGTTTAAAAGCAAGTTTGTC+GGG + Chr5:3598970-3598989 MsG0580024343.01.T01:intron 30.0%
ATATGGTAGTACTTAGATAC+GGG + Chr5:3599244-3599263 MsG0580024343.01.T01:intron 30.0%
ATTTACAATTGCTGATGAAG+GGG + Chr5:3600936-3600955 MsG0580024343.01.T01:intron 30.0%
CATTTACAATTGCTGATGAA+GGG + Chr5:3600935-3600954 MsG0580024343.01.T01:intron 30.0%
GAATATAAGAGAGATTCATC+GGG - Chr5:3600676-3600695 None:intergenic 30.0%
GAGAATCAGAATATTTAGGT+GGG + Chr5:3598764-3598783 MsG0580024343.01.T01:intron 30.0%
GAGGAAAAAGAAAGAGTTTA+TGG - Chr5:3598575-3598594 None:intergenic 30.0%
GCTGTAACAGTAAAAAGTTA+TGG - Chr5:3599883-3599902 None:intergenic 30.0%
GGAACAGTTATTAATTGGTT+AGG - Chr5:3598856-3598875 None:intergenic 30.0%
TACATCTATATCGAACCATT+GGG - Chr5:3601342-3601361 None:intergenic 30.0%
TCATTTACAATTGCTGATGA+AGG + Chr5:3600934-3600953 MsG0580024343.01.T01:intron 30.0%
TCCTCTAGTGATATTACTAT+TGG - Chr5:3599286-3599305 None:intergenic 30.0%
TGAAATTGTTTACACTTCGA+GGG + Chr5:3599641-3599660 MsG0580024343.01.T01:CDS 30.0%
TGAGAATCAGAATATTTAGG+TGG + Chr5:3598763-3598782 MsG0580024343.01.T01:intron 30.0%
TGATATTACTATTGGCAACT+TGG - Chr5:3599278-3599297 None:intergenic 30.0%
TGTAGAATATCTTGAGAACA+AGG + Chr5:3599454-3599473 MsG0580024343.01.T01:five_prime_UTR 30.0%
TTACATCTATATCGAACCAT+TGG - Chr5:3601343-3601362 None:intergenic 30.0%
TTGAAATTGTTTACACTTCG+AGG + Chr5:3599640-3599659 MsG0580024343.01.T01:CDS 30.0%
! AACATGTTTTACTCCTTGTT+TGG + Chr5:3601361-3601380 MsG0580024343.01.T01:three_prime_UTR 30.0%
! AATCACCTCTAAAGAAATAG+TGG - Chr5:3598877-3598896 None:intergenic 30.0%
! AATGGATTTCTTTCATTCGA+AGG - Chr5:3598460-3598479 None:intergenic 30.0%
! CGCTCTTTTATTCCTTTTTA+AGG + Chr5:3601257-3601276 MsG0580024343.01.T01:intron 30.0%
! CTGGAACTCATAAATCTTTT+AGG + Chr5:3599492-3599511 MsG0580024343.01.T01:five_prime_UTR 30.0%
! GTTTCTTTTGTCATTGATGA+AGG + Chr5:3599727-3599746 MsG0580024343.01.T01:intron 30.0%
!! ACATGTTTTACTCCTTGTTT+GGG + Chr5:3601362-3601381 MsG0580024343.01.T01:three_prime_UTR 30.0%
!! ACCAATATTTTTCGGATGAT+TGG + Chr5:3600908-3600927 MsG0580024343.01.T01:intron 30.0%
!! ATCAGAACTTGTTGATTTAG+AGG + Chr5:3599823-3599842 MsG0580024343.01.T01:CDS 30.0%
!! ATCTTTTAGGCATTGTTTTC+TGG + Chr5:3599505-3599524 MsG0580024343.01.T01:five_prime_UTR 30.0%
ACATCAACAATGCTAGAAGT+TGG - Chr5:3599323-3599342 None:intergenic 35.0%
ACATTGAACATGACAAAGCT+AGG + Chr5:3600051-3600070 MsG0580024343.01.T01:intron 35.0%
ATATTTAGGTGGGGGAAAAT+CGG + Chr5:3598774-3598793 MsG0580024343.01.T01:intron 35.0%
ATGATACCGATGTAGATGAT+TGG + Chr5:3600533-3600552 MsG0580024343.01.T01:CDS 35.0%
CAAGTGATTGTCTTAGTAGT+AGG + Chr5:3600110-3600129 MsG0580024343.01.T01:intron 35.0%
CTTCTCTTCTCTTCTTAACA+CGG + Chr5:3598517-3598536 MsG0580024343.01.T01:five_prime_UTR 35.0%
GAATCAGAATATTTAGGTGG+GGG + Chr5:3598766-3598785 MsG0580024343.01.T01:intron 35.0%
GAGCTGTATTTCTCACTTTA+AGG + Chr5:3600644-3600663 MsG0580024343.01.T01:intron 35.0%
GCCAATAGTAATATCACTAG+AGG + Chr5:3599282-3599301 MsG0580024343.01.T01:intron 35.0%
GCCAATCATCCGAAAAATAT+TGG - Chr5:3600912-3600931 None:intergenic 35.0%
GCTATTAGTAGAAGAAGTGA+TGG + Chr5:3599021-3599040 MsG0580024343.01.T01:intron 35.0%
GGTTCGGATGTATAAATATC+CGG - Chr5:3600376-3600395 None:intergenic 35.0%
TCTACATCGGTATCATCAAT+AGG - Chr5:3600529-3600548 None:intergenic 35.0%
TCTAGCATTGTTGATGTTTC+AGG + Chr5:3599326-3599345 MsG0580024343.01.T01:intron 35.0%
TGAGTTCCAGATTCAGTAAT+AGG - Chr5:3599482-3599501 None:intergenic 35.0%
TGTAGTTAGTTAGTTGTACC+TGG - Chr5:3598548-3598567 None:intergenic 35.0%
TTAAGAAGAGAAGAGAAGAG+AGG - Chr5:3598515-3598534 None:intergenic 35.0%
TTTACAATTGCTGATGAAGG+GGG + Chr5:3600937-3600956 MsG0580024343.01.T01:intron 35.0%
! AGTCAAATAGCAGTTAGCTT+TGG + Chr5:3599979-3599998 MsG0580024343.01.T01:intron 35.0%
! CACGCTTAATACTTGGTAAT+TGG - Chr5:3601021-3601040 None:intergenic 35.0%
! CTGTTCCACTATTTCTTTAG+AGG + Chr5:3598869-3598888 MsG0580024343.01.T01:intron 35.0%
! TCACACCCTAAAAGGATTTT+TGG + Chr5:3599193-3599212 MsG0580024343.01.T01:intron 35.0%
!! AAAAAGTATTCCCCAGATAG+GGG - Chr5:3600271-3600290 None:intergenic 35.0%
!! ACAAAAAGTATTCCCCAGAT+AGG - Chr5:3600273-3600292 None:intergenic 35.0%
!! ACTTGTTGATTTAGAGGAGA+AGG + Chr5:3599829-3599848 MsG0580024343.01.T01:CDS 35.0%
!! ATGTTTTACTCCTTGTTTGG+GGG + Chr5:3601364-3601383 MsG0580024343.01.T01:three_prime_UTR 35.0%
!! CAAAAAGTATTCCCCAGATA+GGG - Chr5:3600272-3600291 None:intergenic 35.0%
!! CACTCTCAAGTTTTAACATC+TGG + Chr5:3599431-3599450 MsG0580024343.01.T01:five_prime_UTR 35.0%
!! CATGTTTTACTCCTTGTTTG+GGG + Chr5:3601363-3601382 MsG0580024343.01.T01:three_prime_UTR 35.0%
!! CGCACAATCTAGCTTATTTT+GGG + Chr5:3599569-3599588 MsG0580024343.01.T01:five_prime_UTR 35.0%
!! GTCAAATAGCAGTTAGCTTT+GGG + Chr5:3599980-3599999 MsG0580024343.01.T01:intron 35.0%
!! TCGCACAATCTAGCTTATTT+TGG + Chr5:3599568-3599587 MsG0580024343.01.T01:five_prime_UTR 35.0%
!! TGCAAGATTGGTTTGCAATA+TGG + Chr5:3599227-3599246 MsG0580024343.01.T01:intron 35.0%
!! TTTAGGCATTGTTTTCTGGT+GGG + Chr5:3599509-3599528 MsG0580024343.01.T01:five_prime_UTR 35.0%
!! TTTTAGGCATTGTTTTCTGG+TGG + Chr5:3599508-3599527 MsG0580024343.01.T01:five_prime_UTR 35.0%
!!! AAATCCTTTTAGGGTGTGAT+TGG - Chr5:3599192-3599211 None:intergenic 35.0%
AACACTGAATTTCAACTCCG+AGG - Chr5:3600230-3600249 None:intergenic 40.0%
AAGAGAGATTCATCGGGTAA+AGG - Chr5:3600670-3600689 None:intergenic 40.0%
AAGGTTTGGGCAGTTAAGTA+CGG - Chr5:3600397-3600416 None:intergenic 40.0%
ACATCGGTATCATCAATAGG+TGG - Chr5:3600526-3600545 None:intergenic 40.0%
ACCACGAATCCACCTTAAAA+AGG - Chr5:3601272-3601291 None:intergenic 40.0%
ACCTATCCAGAGGAAGATAA+TGG - Chr5:3599376-3599395 None:intergenic 40.0%
AGGAAGCCAATCATCTACAT+CGG - Chr5:3600542-3600561 None:intergenic 40.0%
ATCTCAAATTCCCCCAAACA+AGG - Chr5:3601377-3601396 None:intergenic 40.0%
ATTGGATGGAGGGTTTAAGA+GGG - Chr5:3599174-3599193 None:intergenic 40.0%
CAATCAGTACTAAGCGTGTT+AGG - Chr5:3600455-3600474 None:intergenic 40.0%
CAGATGACCTTAAGTTCTCA+AGG - Chr5:3599407-3599426 None:intergenic 40.0%
CATGACAAAGCTAGGATTTC+AGG + Chr5:3600059-3600078 MsG0580024343.01.T01:intron 40.0%
CCAAGTATTAAGCGTGAAGA+TGG + Chr5:3601025-3601044 MsG0580024343.01.T01:CDS 40.0%
CCATCTTCACGCTTAATACT+TGG - Chr5:3601028-3601047 None:intergenic 40.0%
GGCATTTCCTTGAGAACTTA+AGG + Chr5:3599397-3599416 MsG0580024343.01.T01:five_prime_UTR 40.0%
GTGACTAGACTCCAAATCTA+TGG - Chr5:3600091-3600110 None:intergenic 40.0%
GTTAGGTAATGACAAGCAGA+TGG - Chr5:3600438-3600457 None:intergenic 40.0%
TCTACTAATAGCAAGCTCAC+CGG - Chr5:3599013-3599032 None:intergenic 40.0%
TTATCGCCATTATCTTCCTC+TGG + Chr5:3599367-3599386 MsG0580024343.01.T01:five_prime_UTR 40.0%
TTATCTTCCTCTGGATAGGT+TGG + Chr5:3599376-3599395 MsG0580024343.01.T01:five_prime_UTR 40.0%
TTGTTTGGGGGAATTTGAGA+TGG + Chr5:3601376-3601395 MsG0580024343.01.T01:three_prime_UTR 40.0%
! AAGGCACCTATTACTGAATC+TGG + Chr5:3599473-3599492 MsG0580024343.01.T01:five_prime_UTR 40.0%
! GGAAAAGGTTTGCTTTCATC+AGG + Chr5:3599693-3599712 MsG0580024343.01.T01:intron 40.0%
! TCAGGTTCTAGCCATAGATT+TGG + Chr5:3600077-3600096 MsG0580024343.01.T01:intron 40.0%
!! CATTGTTTTCTGGTGGGAAA+GGG + Chr5:3599515-3599534 MsG0580024343.01.T01:five_prime_UTR 40.0%
!! CTAACACGCTTAGTACTGAT+TGG + Chr5:3600453-3600472 MsG0580024343.01.T01:intron 40.0%
!! TGTTGATTTAGAGGAGAAGG+AGG + Chr5:3599832-3599851 MsG0580024343.01.T01:CDS 40.0%
!!! ATCTAGCTTATTTTGGGCCT+TGG + Chr5:3599575-3599594 MsG0580024343.01.T01:five_prime_UTR 40.0%
!!! TCCTTTTTAAGGTGGATTCG+TGG + Chr5:3601268-3601287 MsG0580024343.01.T01:intron 40.0%
AGAAGAGAGGACGAAAACGA+AGG - Chr5:3598502-3598521 None:intergenic 45.0%
AGGGTTTAAGAGGGGAAGAT+AGG - Chr5:3599165-3599184 None:intergenic 45.0%
CAAAGGAAGCTACTTGACCT+CGG + Chr5:3600210-3600229 MsG0580024343.01.T01:intron 45.0%
CCATCCAATCACACCCTAAA+AGG + Chr5:3599185-3599204 MsG0580024343.01.T01:intron 45.0%
GAGGACTTGAAGAACACCAA+GGG + Chr5:3599606-3599625 MsG0580024343.01.T01:five_prime_UTR 45.0%
GATTGGATGGAGGGTTTAAG+AGG - Chr5:3599175-3599194 None:intergenic 45.0%
GCCATTATCTTCCTCTGGAT+AGG + Chr5:3599372-3599391 MsG0580024343.01.T01:five_prime_UTR 45.0%
GGACGAAAACGAAGGTAAGT+AGG - Chr5:3598494-3598513 None:intergenic 45.0%
GGGTTTAAGAGGGGAAGATA+GGG - Chr5:3599164-3599183 None:intergenic 45.0%
GGTTTAAGAGGGGAAGATAG+GGG - Chr5:3599163-3599182 None:intergenic 45.0%
GTGGTTATCAGCTCTGCAAT+CGG + Chr5:3601287-3601306 MsG0580024343.01.T01:CDS 45.0%
TAGTAGTGGTAGTCGGGAAA+AGG + Chr5:3599678-3599697 MsG0580024343.01.T01:CDS 45.0%
TCAATAGGTGGAGATTCTCC+TGG - Chr5:3600514-3600533 None:intergenic 45.0%
TCTGATGCATCAACTCCCAA+TGG + Chr5:3601324-3601343 MsG0580024343.01.T01:CDS 45.0%
TTAGGGTGTGATTGGATGGA+GGG - Chr5:3599184-3599203 None:intergenic 45.0%
TTAGTTAGTTGTACCTGGCC+TGG - Chr5:3598543-3598562 None:intergenic 45.0%
TTCAACATGAAAGCAGCCCT+TGG - Chr5:3599625-3599644 None:intergenic 45.0%
TTGGATGGAGGGTTTAAGAG+GGG - Chr5:3599173-3599192 None:intergenic 45.0%
TTGGGCAGTTAAGTACGGTT+CGG - Chr5:3600392-3600411 None:intergenic 45.0%
TTTCCCGACTACCACTACTA+GGG - Chr5:3599678-3599697 None:intergenic 45.0%
! CTCGCGTTTTCGTAAGCATA+AGG - Chr5:3600815-3600834 None:intergenic 45.0%
! GGACTGCTAGTCTAACAATC+CGG + Chr5:3598991-3599010 MsG0580024343.01.T01:intron 45.0%
! TGAAGTTTAGGGCTGATCGA+TGG + Chr5:3598700-3598719 MsG0580024343.01.T01:intron 45.0%
! TTTAGGGTGTGATTGGATGG+AGG - Chr5:3599185-3599204 None:intergenic 45.0%
! TTTTCCCGACTACCACTACT+AGG - Chr5:3599679-3599698 None:intergenic 45.0%
!! CCTCGCTTTGTTGTTCTTCA+GGG + Chr5:3600559-3600578 MsG0580024343.01.T01:CDS 45.0%
!! CTCGAGCAGTTCTAAGATAC+AGG + Chr5:3601102-3601121 MsG0580024343.01.T01:CDS 45.0%
!! CTTGGTGTTCTTCAAGTCCT+CGG - Chr5:3599607-3599626 None:intergenic 45.0%
!! GATAGAAGTGTTGAGTCGTG+AGG - Chr5:3600980-3600999 None:intergenic 45.0%
!! GCATTGTTTTCTGGTGGGAA+AGG + Chr5:3599514-3599533 MsG0580024343.01.T01:five_prime_UTR 45.0%
!! TCCTCGCTTTGTTGTTCTTC+AGG + Chr5:3600558-3600577 MsG0580024343.01.T01:CDS 45.0%
!!! CCTTTTAGGGTGTGATTGGA+TGG - Chr5:3599188-3599207 None:intergenic 45.0%
AAGTATTCCCCAGATAGGGG+AGG - Chr5:3600268-3600287 None:intergenic 50.0%
AGTATTCCCCAGATAGGGGA+GGG - Chr5:3600267-3600286 None:intergenic 50.0%
CCCTGAAGAACAACAAAGCG+AGG - Chr5:3600562-3600581 None:intergenic 50.0%
CGAGGACTTGAAGAACACCA+AGG + Chr5:3599605-3599624 MsG0580024343.01.T01:five_prime_UTR 50.0%
CTTCTCTTCTTAACACGGCC+AGG + Chr5:3598522-3598541 MsG0580024343.01.T01:five_prime_UTR 50.0%
GGAAATGCCAACCTATCCAG+AGG - Chr5:3599386-3599405 None:intergenic 50.0%
GTGTTCTTCAAGTCCTCGGA+TGG - Chr5:3599603-3599622 None:intergenic 50.0%
TAAGAGGGGAAGATAGGGGA+GGG - Chr5:3599159-3599178 None:intergenic 50.0%
TAGGTGGGGGAAAATCGGTA+TGG + Chr5:3598779-3598798 MsG0580024343.01.T01:intron 50.0%
TCTGCAATCGGACTGCAAAC+TGG + Chr5:3601299-3601318 MsG0580024343.01.T01:CDS 50.0%
TTAAGAGGGGAAGATAGGGG+AGG - Chr5:3599160-3599179 None:intergenic 50.0%
CAGAGATGGACAGAGCTTCC+AGG + Chr5:3600493-3600512 MsG0580024343.01.T01:intron 55.0%
CTACCACTACTAGGGCTCCA+TGG - Chr5:3599670-3599689 None:intergenic 55.0%
GAGCCCTAGTAGTGGTAGTC+GGG + Chr5:3599672-3599691 MsG0580024343.01.T01:CDS 55.0%
GGAGCCCTAGTAGTGGTAGT+CGG + Chr5:3599671-3599690 MsG0580024343.01.T01:CDS 55.0%
TATCCAGCCCTCCCCTATCT+GGG + Chr5:3600257-3600276 MsG0580024343.01.T01:intron 55.0%
TTACACTTCGAGGGCTGCCA+TGG + Chr5:3599650-3599669 MsG0580024343.01.T01:CDS 55.0%
TTATCCAGCCCTCCCCTATC+TGG + Chr5:3600256-3600275 MsG0580024343.01.T01:intron 55.0%
TTGCGGTCAGCTCGTCTATC+CGG + Chr5:3600354-3600373 MsG0580024343.01.T01:CDS 55.0%
ATCCAGCCCTCCCCTATCTG+GGG + Chr5:3600258-3600277 MsG0580024343.01.T01:intron 60.0%
CAAGTCCTCGGATGGCACCA+AGG - Chr5:3599595-3599614 None:intergenic 60.0%
CTGCCATGGAGCCCTAGTAG+TGG + Chr5:3599664-3599683 MsG0580024343.01.T01:CDS 60.0%
CTTCTTAACACGGCCAGGCC+AGG + Chr5:3598527-3598546 MsG0580024343.01.T01:five_prime_UTR 60.0%
GGCGTGTCGTACTCGTACGT+AGG + Chr5:3598425-3598444 MsG0580024343.01.T01:five_prime_UTR 60.0%
TTCCCCAGATAGGGGAGGGC+TGG - Chr5:3600263-3600282 None:intergenic 65.0%
!! TTGGGCCTTGGTGCCATCCG+AGG + Chr5:3599587-3599606 MsG0580024343.01.T01:five_prime_UTR 65.0%
Chromosome Type Strat End Strand Name
Chr5 gene 3598417 3601509 3598417 ID=MsG0580024343.01;Name=MsG0580024343.01
Chr5 mRNA 3598417 3601509 3598417 ID=MsG0580024343.01.T01;Parent=MsG0580024343.01;Name=MsG0580024343.01.T01;_AED=0.33;_eAED=0.33;_QI=421|0.83|0.85|1|0.83|0.71|7|153|175
Chr5 exon 3598417 3598548 3598417 ID=MsG0580024343.01.T01:exon:2058;Parent=MsG0580024343.01.T01
Chr5 exon 3599348 3599699 3599348 ID=MsG0580024343.01.T01:exon:2059;Parent=MsG0580024343.01.T01
Chr5 exon 3599788 3599870 3599788 ID=MsG0580024343.01.T01:exon:2060;Parent=MsG0580024343.01.T01
Chr5 exon 3600332 3600395 3600332 ID=MsG0580024343.01.T01:exon:2061;Parent=MsG0580024343.01.T01
Chr5 exon 3600496 3600580 3600496 ID=MsG0580024343.01.T01:exon:2062;Parent=MsG0580024343.01.T01
Chr5 exon 3600969 3601123 3600969 ID=MsG0580024343.01.T01:exon:2063;Parent=MsG0580024343.01.T01
Chr5 exon 3601279 3601509 3601279 ID=MsG0580024343.01.T01:exon:2064;Parent=MsG0580024343.01.T01
Chr5 five_prime_UTR 3598417 3598548 3598417 ID=MsG0580024343.01.T01:five_prime_utr;Parent=MsG0580024343.01.T01
Chr5 five_prime_UTR 3599348 3599636 3599348 ID=MsG0580024343.01.T01:five_prime_utr;Parent=MsG0580024343.01.T01
Chr5 CDS 3599637 3599699 3599637 ID=MsG0580024343.01.T01:cds;Parent=MsG0580024343.01.T01
Chr5 CDS 3599788 3599870 3599788 ID=MsG0580024343.01.T01:cds;Parent=MsG0580024343.01.T01
Chr5 CDS 3600332 3600395 3600332 ID=MsG0580024343.01.T01:cds;Parent=MsG0580024343.01.T01
Chr5 CDS 3600496 3600580 3600496 ID=MsG0580024343.01.T01:cds;Parent=MsG0580024343.01.T01
Chr5 CDS 3600969 3601123 3600969 ID=MsG0580024343.01.T01:cds;Parent=MsG0580024343.01.T01
Chr5 CDS 3601279 3601356 3601279 ID=MsG0580024343.01.T01:cds;Parent=MsG0580024343.01.T01
Chr5 three_prime_UTR 3601357 3601509 3601357 ID=MsG0580024343.01.T01:three_prime_utr;Parent=MsG0580024343.01.T01
Gene Sequence

>MsG0580024343.01.T01

ATGTTGAAATTGTTTACACTTCGAGGGCTGCCATGGAGCCCTAGTAGTGGTAGTCGGGAAAAGGTTGAACTAACAGCTGCAGAAGTAGAGTCTCTTCGATCAGAACTTGTTGATTTAGAGGAGAAGGAGGCTCAGTTAAAAGCTCAGTTGGAAAATATTGATGAAATTTTGCGGTCAGCTCGTCTATCCGGATATTTATACATCCGAACCAGATGGACAGAGCTTCCAGGAGAATCTCCACCTATTGATGATACCGATGTAGATGATTGGCTTCCTCGCTTTGTTGTTCTTCAGGACTTAAGTCCTCACGACTCAACACTTCTATCTGACATTGTTGAAGTATACCAATTACCAAGTATTAAGCGTGAAGATGGTGAGATGAGATACGCCTTTTGTATTTTAACACGACATGGATTACGATATGAATGCTCGAGCAGTTCTAAGATACAGGTGGATTCGTGGTTATCAGCTCTGCAATCGGACTGCAAACTGGAATCTGATGCATCAACTCCCAATGGTTCGATATAG
Protein sequence

>MsG0580024343.01.T01

MLKLFTLRGLPWSPSSGSREKVELTAAEVESLRSELVDLEEKEAQLKAQLENIDEILRSARLSGYLYIRTRWTELPGESPPIDDTDVDDWLPRFVVLQDLSPHDSTLLSDIVEVYQLPSIKREDGEMRYAFCILTRHGLRYECSSSSKIQVDSWLSALQSDCKLESDASTPNGSI*