AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580024352.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580024352.01.T01 MTR_5g009500 55.775 355 63 10 5 274 1 346 2.08e-111 325
MsG0580024352.01.T01 MTR_8g027975 44.262 366 100 12 4 274 5 361 5.47e-78 241
MsG0580024352.01.T01 MTR_5g081150 26.736 288 143 10 29 262 22 295 9.33e-16 76.3
MsG0580024352.01.T01 MTR_1g012030 27.477 222 125 4 40 225 137 358 5.07e-14 72.0
MsG0580024352.01.T01 MTR_3g107510 26.126 222 128 5 40 225 120 341 9.79e-13 67.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580024352.01.T01 AT4G01100 42.700 363 104 10 5 274 1 352 9.15e-72 224
MsG0580024352.01.T01 AT4G01100 41.379 377 103 11 5 274 1 366 2.05e-70 221
MsG0580024352.01.T01 AT4G01100 40.514 311 89 8 51 274 1 302 2.85e-55 181
MsG0580024352.01.T01 AT4G26180 30.901 233 109 7 39 220 18 249 1.47e-16 78.2
MsG0580024352.01.T01 AT4G26180 30.901 233 109 7 39 220 18 249 2.20e-16 78.2
MsG0580024352.01.T01 AT3G53940 26.087 276 141 7 39 262 70 334 1.28e-13 70.5
MsG0580024352.01.T01 AT5G51050 29.555 247 114 11 25 219 204 442 1.02e-12 68.2
MsG0580024352.01.T01 AT4G32400 25.664 226 124 4 40 225 112 333 2.94e-12 66.6

Find 77 sgRNAs with CRISPR-Local

Find 211 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCTTCTGCGTCTTGGAGCT+GGG 0.252032 5:+3753583 MsG0580024352.01.T01:CDS
CCTCTTCTGCGTCTTGGAGC+TGG 0.254698 5:+3753582 MsG0580024352.01.T01:CDS
ATCCTAAGATGATATTCTTA+TGG 0.263272 5:+3754914 MsG0580024352.01.T01:three_prime_UTR
CTTGCATGTGGGGCTGCTGC+TGG 0.296448 5:+3754106 MsG0580024352.01.T01:CDS
CATATGGTACTATAAGCTTA+AGG 0.353640 5:-3752706 None:intergenic
ATGCTCTTTCAACAGTCTTC+CGG 0.365311 5:+3753830 MsG0580024352.01.T01:CDS
TACCTACCTTTCAAACTTCA+AGG 0.367732 5:+3753494 MsG0580024352.01.T01:CDS
CGAGATGGTCAAGACGTTCT+AGG 0.368677 5:+3754692 MsG0580024352.01.T01:CDS
ATCCATAAGAATATCATCTT+AGG 0.371021 5:-3754916 None:intergenic
GGGGCTGCTGCTGGAACTAT+TGG 0.396252 5:+3754115 MsG0580024352.01.T01:CDS
GGCGCTAGAGCATTGTATAA+AGG 0.419300 5:+3753858 MsG0580024352.01.T01:CDS
CTCACTCCTCTTCTGCGTCT+TGG 0.425612 5:+3753576 MsG0580024352.01.T01:CDS
ATTTCAGCTTCAAGCTTCAA+TGG 0.430101 5:+3751384 None:intergenic
AAAAGCGTGATTAGGAGGTT+TGG 0.431176 5:-3751473 None:intergenic
TTCAACAGTCTTCCGGAAGA+AGG 0.433762 5:+3753837 MsG0580024352.01.T01:CDS
ATATGGTACTATAAGCTTAA+GGG 0.436373 5:-3752705 None:intergenic
CTTGGAGCTGGGGCATGTGC+AGG 0.442547 5:+3753594 MsG0580024352.01.T01:CDS
AGGAGGTTTGGCACCTTCAC+CGG 0.455797 5:-3751461 None:intergenic
ATACTGAGAAAAGCGTGATT+AGG 0.460163 5:-3751481 None:intergenic
GCTTCCGTTGTCACTGGTGA+CGG 0.466067 5:+3754205 MsG0580024352.01.T01:CDS
TTAACAGTACCTGTACAGTT+AGG 0.476691 5:-3753665 None:intergenic
AATGCTCTAGCGCCTTCTTC+CGG 0.484272 5:-3753849 None:intergenic
CGTCCTCGCACCAGGTCCAT+CGG 0.488129 5:-3753642 None:intergenic
GGCACCTTCACCGGCGATTG+CGG 0.488284 5:-3751452 None:intergenic
CTTCCGTTGTCACTGGTGAC+GGG 0.490939 5:+3754206 MsG0580024352.01.T01:CDS
GTCCTCGCACCAGGTCCATC+GGG 0.496178 5:-3753641 None:intergenic
GGCGCTGCTTCCGTTGTCAC+TGG 0.499991 5:+3754199 MsG0580024352.01.T01:CDS
CTTGTCGCAGGTGGAGTCGC+CGG 0.500084 5:+3751514 MsG0580024352.01.T01:CDS
GAGATTGAAGATTCTGCTGC+AGG 0.501355 5:+3752677 MsG0580024352.01.T01:CDS
TTCTTCTGCGAATGACATCA+AGG 0.504967 5:-3754156 None:intergenic
ATGCAGATGGGAGGCTGGAA+AGG 0.510793 5:+3754178 MsG0580024352.01.T01:CDS
GAAGAATGCAGATGGGAGGC+TGG 0.511460 5:+3754173 MsG0580024352.01.T01:CDS
TGTCACGAAGGCAATGGCTA+TGG 0.511840 5:-3754667 None:intergenic
CCAGCTCCAAGACGCAGAAG+AGG 0.514439 5:-3753582 None:intergenic
CAGTTAGGCGTCCTCGCACC+AGG 0.518084 5:-3753650 None:intergenic
CGTTGTCACTGGTGACGGGA+AGG 0.519437 5:+3754210 MsG0580024352.01.T01:CDS
GTCTCCGCAATCGCCGGTGA+AGG 0.536172 5:+3751448 MsG0580024352.01.T01:CDS
CTGAGAAAAGCGTGATTAGG+AGG 0.537877 5:-3751478 None:intergenic
ATCTGCAAATCTCTTGTCGC+AGG 0.542029 5:+3751502 MsG0580024352.01.T01:CDS
ATGGCGTCTCAAGATTCAGC+TGG 0.547242 5:+3751403 MsG0580024352.01.T01:CDS
CATTCGCAGAAGAATGCAGA+TGG 0.551827 5:+3754165 MsG0580024352.01.T01:CDS
TCTAAAGTCTCCGCAATCGC+CGG 0.551921 5:+3751442 MsG0580024352.01.T01:CDS
TGCAAATCTCTTGTCGCAGG+TGG 0.557858 5:+3751505 MsG0580024352.01.T01:CDS
TGACACAAGGCTTGCATGTG+GGG 0.558542 5:+3754096 MsG0580024352.01.T01:CDS
CGCAACTTACCCGATGGACC+TGG 0.558753 5:+3753632 MsG0580024352.01.T01:CDS
AGCCTTGAAGTTTGAAAGGT+AGG 0.566836 5:-3753496 None:intergenic
ACATAGCCTTGAAGTTTGAA+AGG 0.568183 5:-3753500 None:intergenic
GCGAGGACGCCTAACTGTAC+AGG 0.574869 5:+3753656 MsG0580024352.01.T01:CDS
AAAATTCCTCTGTATTGATG+AGG 0.577478 5:-3753805 None:intergenic
GTTGTCACTGGTGACGGGAA+GGG 0.578565 5:+3754211 MsG0580024352.01.T01:CDS
ACGAAGGCAATGGCTATGGA+AGG 0.582467 5:-3754663 None:intergenic
TCTTCTGCGTCTTGGAGCTG+GGG 0.588235 5:+3753584 MsG0580024352.01.T01:CDS
CTCGTATGTCACGAAGGCAA+TGG 0.590817 5:-3754673 None:intergenic
AAGTGAAACTCACACTCCAC+CGG 0.598603 5:-3751533 None:intergenic
CTAGAGCATTGTATAAAGGA+TGG 0.598668 5:+3753862 MsG0580024352.01.T01:CDS
GTCGCAGGTGGAGTCGCCGG+TGG 0.605211 5:+3751517 MsG0580024352.01.T01:CDS
TCTTCTGCGAATGACATCAA+GGG 0.610404 5:-3754155 None:intergenic
AATTATTTACATAAAATCAG+TGG 0.611486 5:-3754876 None:intergenic
TACCCGATGGACCTGGTGCG+AGG 0.618370 5:+3753639 MsG0580024352.01.T01:CDS
TGCCTTCGTGACATACGAGA+TGG 0.618477 5:+3754677 MsG0580024352.01.T01:CDS
CTTCTGCGAATGACATCAAG+GGG 0.618902 5:-3754154 None:intergenic
CGCAGAAGAATGCAGATGGG+AGG 0.620203 5:+3754169 MsG0580024352.01.T01:CDS
GCAGAATCTTCAATCTCTAG+AGG 0.625838 5:-3752671 None:intergenic
TAATCTTGAGTACACTGGCA+TGG 0.633949 5:+3754234 MsG0580024352.01.T01:CDS
GTGACACAAGGCTTGCATGT+GGG 0.645429 5:+3754095 MsG0580024352.01.T01:CDS
AATGTCCGCAACTTACCCGA+TGG 0.649760 5:+3753626 MsG0580024352.01.T01:CDS
AAGGGTAATCTTGAGTACAC+TGG 0.650989 5:+3754229 MsG0580024352.01.T01:CDS
CTTCCCGTCACCAGTGACAA+CGG 0.653118 5:-3754209 None:intergenic
GACCATCTCGTATGTCACGA+AGG 0.657725 5:-3754679 None:intergenic
CAGGTCCATCGGGTAAGTTG+CGG 0.663278 5:-3753631 None:intergenic
CGAAGGCAATGGCTATGGAA+GGG 0.663950 5:-3754662 None:intergenic
GCATCTCCTCATCAATACAG+AGG 0.672044 5:+3753799 MsG0580024352.01.T01:CDS
TGGAACTATTGGCCAGACAG+TGG 0.674835 5:+3754126 MsG0580024352.01.T01:CDS
ATTCGCAGAAGAATGCAGAT+GGG 0.675451 5:+3754166 MsG0580024352.01.T01:CDS
TGTGACACAAGGCTTGCATG+TGG 0.691984 5:+3754094 MsG0580024352.01.T01:CDS
TCAGAGCTAAGTGTGACACA+AGG 0.730696 5:+3754083 MsG0580024352.01.T01:CDS
TTCTGCGAATGACATCAAGG+GGG 0.801026 5:-3754153 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATATAATATAATATAATAA+AGG - Chr5:3751770-3751789 None:intergenic 0.0%
!! ATAATATAATATAATAAAGG+CGG - Chr5:3751767-3751786 None:intergenic 10.0%
!!! AATTTTTTTTTATCTGAATA+TGG + Chr5:3753396-3753415 MsG0580024352.01.T01:intron 10.0%
!! AAATAGTAATAGTTAAAAAG+TGG + Chr5:3754584-3754603 MsG0580024352.01.T01:intron 15.0%
!! AATTATGTTTGTAGAATTTA+AGG + Chr5:3753703-3753722 MsG0580024352.01.T01:intron 15.0%
!! AATTATTTACATAAAATCAG+TGG - Chr5:3754879-3754898 None:intergenic 15.0%
!! AGATGTATAAAGATTATTAA+GGG - Chr5:3753268-3753287 None:intergenic 15.0%
!! ATAGAATATTTACTTTATCA+TGG + Chr5:3753316-3753335 MsG0580024352.01.T01:intron 15.0%
!! ATGATTTAAATCATTTATCT+AGG + Chr5:3754397-3754416 MsG0580024352.01.T01:intron 15.0%
!! ATTTAATCTTCTGATTATTT+AGG + Chr5:3751694-3751713 MsG0580024352.01.T01:intron 15.0%
!! GAAAAAAAAATATTGCATAT+AGG + Chr5:3754826-3754845 MsG0580024352.01.T01:three_prime_UTR 15.0%
!! GATATTTAATGTATTAACAT+AGG + Chr5:3752732-3752751 MsG0580024352.01.T01:intron 15.0%
!! TACATTAAATATCTAACATA+TGG - Chr5:3752725-3752744 None:intergenic 15.0%
!! TAGATGTATAAAGATTATTA+AGG - Chr5:3753269-3753288 None:intergenic 15.0%
!! TGAAAATGAATTAAACTTAA+TGG - Chr5:3753757-3753776 None:intergenic 15.0%
!!! ATTAATATTTTCTCTCTATT+TGG + Chr5:3752007-3752026 MsG0580024352.01.T01:intron 15.0%
!!! ATTTTTATTCATATTATGTG+AGG + Chr5:3753466-3753485 MsG0580024352.01.T01:CDS 15.0%
!!! CATTTTTATATGCTTAAAAT+AGG + Chr5:3752588-3752607 MsG0580024352.01.T01:intron 15.0%
!!! GAGCTATTAATTATTTATTT+TGG + Chr5:3751854-3751873 MsG0580024352.01.T01:intron 15.0%
!!! TAAGTTTAATTCATTTTCAA+TGG + Chr5:3753758-3753777 MsG0580024352.01.T01:intron 15.0%
!!! TTTTTATTCATATTATGTGA+GGG + Chr5:3753467-3753486 MsG0580024352.01.T01:CDS 15.0%
!!! TTTTTTTTTCCATTAATCTT+TGG + Chr5:3753142-3753161 MsG0580024352.01.T01:intron 15.0%
!! AAAATATATATGGAAAACTG+AGG + Chr5:3753009-3753028 MsG0580024352.01.T01:intron 20.0%
!! AAATACAGAAACAGAAAAAT+GGG - Chr5:3751735-3751754 None:intergenic 20.0%
!! AAATATATATGGAAAACTGA+GGG + Chr5:3753010-3753029 MsG0580024352.01.T01:intron 20.0%
!! AAGCAATTAATTAATGCAAA+TGG - Chr5:3754359-3754378 None:intergenic 20.0%
!! AGAACACAAAATTCATTTAT+AGG + Chr5:3754558-3754577 MsG0580024352.01.T01:intron 20.0%
!! AGCAATTAATTAATGCAAAT+GGG - Chr5:3754358-3754377 None:intergenic 20.0%
!! ATAAAAATAAAGTGTGACAA+TGG - Chr5:3753526-3753545 None:intergenic 20.0%
!! ATTTAAATCATTTATCTAGG+TGG + Chr5:3754400-3754419 MsG0580024352.01.T01:intron 20.0%
!! CTAAAATTCTAAATTTCTCT+AGG - Chr5:3754464-3754483 None:intergenic 20.0%
!! TAACATTTACCAAAGATTAA+TGG - Chr5:3753154-3753173 None:intergenic 20.0%
!! TAGTTAAAAAGTGGTTTATA+TGG + Chr5:3754593-3754612 MsG0580024352.01.T01:intron 20.0%
!! TTTAAATCATTTATCTAGGT+GGG + Chr5:3754401-3754420 MsG0580024352.01.T01:intron 20.0%
!! TTTGTGAATTATGTTTCATT+TGG + Chr5:3752442-3752461 MsG0580024352.01.T01:intron 20.0%
!!! AAGTTTGAGATTTCTTTATA+TGG - Chr5:3751923-3751942 None:intergenic 20.0%
!!! ATAAGATCATTTTAAAATGC+AGG + Chr5:3752940-3752959 MsG0580024352.01.T01:intron 20.0%
!!! ATTATTTTCTCCAATGTTAA+AGG + Chr5:3752767-3752786 MsG0580024352.01.T01:intron 20.0%
!!! ATTTAGAATTTTAGATACAG+TGG + Chr5:3754470-3754489 MsG0580024352.01.T01:intron 20.0%
!!! TGATTATTTTATTCTGTCAA+TGG + Chr5:3753349-3753368 MsG0580024352.01.T01:intron 20.0%
!!! TTTTATTCATATTATGTGAG+GGG + Chr5:3753468-3753487 MsG0580024352.01.T01:CDS 20.0%
! AAAAAAAATAGATTCACACG+TGG - Chr5:3751818-3751837 None:intergenic 25.0%
! AATGCATTTAAGAAAATGTG+TGG + Chr5:3752860-3752879 MsG0580024352.01.T01:intron 25.0%
! AGAAACATGAAATAAAAGCT+TGG - Chr5:3753954-3753973 None:intergenic 25.0%
! AGTAAAACTGTAAAGTATGA+GGG + Chr5:3754268-3754287 MsG0580024352.01.T01:intron 25.0%
! AGTGATTTCTATAACAAAAC+TGG + Chr5:3752179-3752198 MsG0580024352.01.T01:intron 25.0%
! ATAAAGATTATTAAGGGAGA+AGG - Chr5:3753262-3753281 None:intergenic 25.0%
! ATATGGTACTATAAGCTTAA+GGG - Chr5:3752708-3752727 None:intergenic 25.0%
! ATATTTACTTTATCATGGTG+TGG + Chr5:3753321-3753340 MsG0580024352.01.T01:intron 25.0%
! ATCCATAAGAATATCATCTT+AGG - Chr5:3754919-3754938 None:intergenic 25.0%
! ATCCTAAGATGATATTCTTA+TGG + Chr5:3754914-3754933 MsG0580024352.01.T01:three_prime_UTR 25.0%
! CAACAATAAACCTTTAACAT+TGG - Chr5:3752780-3752799 None:intergenic 25.0%
! GAAATACAGAAACAGAAAAA+TGG - Chr5:3751736-3751755 None:intergenic 25.0%
! TAAAGGTTTATTGTTGATAC+TGG + Chr5:3752784-3752803 MsG0580024352.01.T01:intron 25.0%
! TCAGGGCTTAAAAATATATA+TGG + Chr5:3752999-3753018 MsG0580024352.01.T01:intron 25.0%
! TGAATAAAAATGCAGATTGA+AGG - Chr5:3753457-3753476 None:intergenic 25.0%
! TGTTCTATGACTAACAAAAT+TGG - Chr5:3754544-3754563 None:intergenic 25.0%
! TTGATTAAAACAAAACCACT+TGG + Chr5:3754047-3754066 MsG0580024352.01.T01:intron 25.0%
! TTGTATACAATCAATCTAAG+TGG + Chr5:3752335-3752354 MsG0580024352.01.T01:intron 25.0%
!! CTCAAACTTGAAATGATTTT+TGG + Chr5:3751934-3751953 MsG0580024352.01.T01:intron 25.0%
!! TTATGATTTTGCATTGTGAT+AGG + Chr5:3752559-3752578 MsG0580024352.01.T01:intron 25.0%
!!! GTTTTGTTATAGAAATCACT+TGG - Chr5:3752179-3752198 None:intergenic 25.0%
!!! TAGTTTTGATTTTCATCTCA+AGG - Chr5:3752393-3752412 None:intergenic 25.0%
ACATGAAATAAAAGCTTGGT+TGG - Chr5:3753950-3753969 None:intergenic 30.0%
AGTATAAAACAGTGATCAGA+AGG - Chr5:3753186-3753205 None:intergenic 30.0%
CAAACTAAAGAACTTATCGT+TGG - Chr5:3751638-3751657 None:intergenic 30.0%
CACTGAACAACAAGTAATAT+AGG - Chr5:3752919-3752938 None:intergenic 30.0%
CAGTAAAACTGTAAAGTATG+AGG + Chr5:3754267-3754286 MsG0580024352.01.T01:intron 30.0%
CATATGGTACTATAAGCTTA+AGG - Chr5:3752709-3752728 None:intergenic 30.0%
CATTTAAGAAAATGTGTGGA+TGG + Chr5:3752864-3752883 MsG0580024352.01.T01:intron 30.0%
GAAAAATTTAAACCCACGTA+CGG - Chr5:3754003-3754022 None:intergenic 30.0%
TATACATGTACATATCGCTT+TGG - Chr5:3752306-3752325 None:intergenic 30.0%
TCAATTGCATTCTTCTCTAA+TGG + Chr5:3754628-3754647 MsG0580024352.01.T01:intron 30.0%
TCGTCACATGTAAAATACAA+CGG + Chr5:3752970-3752989 MsG0580024352.01.T01:intron 30.0%
TTATTAAGGGAGAAGGATTT+CGG - Chr5:3753255-3753274 None:intergenic 30.0%
! AAAATTCCTCTGTATTGATG+AGG - Chr5:3753808-3753827 None:intergenic 30.0%
! ACTGTTTTATACTAATGCAG+TGG + Chr5:3753193-3753212 MsG0580024352.01.T01:intron 30.0%
! GACATTCAAGAGTTGTTATT+TGG - Chr5:3752113-3752132 None:intergenic 30.0%
! GTATATGAATCTCTAAAGAC+TGG + Chr5:3754024-3754043 MsG0580024352.01.T01:intron 30.0%
! TCTATCTGCTTTATTAGAGT+TGG - Chr5:3753301-3753320 None:intergenic 30.0%
! TTCAGACCAAAGTTGTTATT+TGG - Chr5:3752208-3752227 None:intergenic 30.0%
!! CATTGACTTGTTATCTTTGT+TGG + Chr5:3753731-3753750 MsG0580024352.01.T01:intron 30.0%
AAAATACAACGGAACAGTTC+AGG + Chr5:3752981-3753000 MsG0580024352.01.T01:intron 35.0%
AAAATGGGATTCACATGGAA+AGG - Chr5:3751720-3751739 None:intergenic 35.0%
AAATACAACGGAACAGTTCA+GGG + Chr5:3752982-3753001 MsG0580024352.01.T01:intron 35.0%
AAGAACTTCACTGCTGAATT+TGG - Chr5:3753084-3753103 None:intergenic 35.0%
AATCATTTATCTAGGTGGGT+AGG + Chr5:3754405-3754424 MsG0580024352.01.T01:intron 35.0%
ACAGAAAAATGGGATTCACA+TGG - Chr5:3751725-3751744 None:intergenic 35.0%
ACTGCAAGGAAACAAAAACA+CGG + Chr5:3754501-3754520 MsG0580024352.01.T01:intron 35.0%
ACTGTAAAGTATGAGGGATT+TGG + Chr5:3754274-3754293 MsG0580024352.01.T01:intron 35.0%
AGCCAATCTATCTCTATTGA+TGG - Chr5:3752495-3752514 None:intergenic 35.0%
AGGGGAATGTTTAAAGGAAA+CGG + Chr5:3753037-3753056 MsG0580024352.01.T01:intron 35.0%
ATAATATAATAAAGGCGGCC+CGG - Chr5:3751762-3751781 None:intergenic 35.0%
ATATGTCAGTCTTGATTACC+AGG + Chr5:3752134-3752153 MsG0580024352.01.T01:intron 35.0%
CAGTGGTATTAAGTCTCTAT+CGG + Chr5:3753210-3753229 MsG0580024352.01.T01:intron 35.0%
CATTCTTCTCTAATGGTTTC+AGG + Chr5:3754635-3754654 MsG0580024352.01.T01:intron 35.0%
GTCCATCAATAGAGATAGAT+TGG + Chr5:3752490-3752509 MsG0580024352.01.T01:intron 35.0%
TAAGAAAATGTGTGGATGGA+TGG + Chr5:3752868-3752887 MsG0580024352.01.T01:intron 35.0%
TACCTACCTTTCAAACTTCA+AGG + Chr5:3753494-3753513 MsG0580024352.01.T01:CDS 35.0%
TATCGTTGGATTGAAGAGAA+CGG - Chr5:3751624-3751643 None:intergenic 35.0%
TCGAGCTAACAACTTGAATA+TGG - Chr5:3753429-3753448 None:intergenic 35.0%
TTAACAGTACCTGTACAGTT+AGG - Chr5:3753668-3753687 None:intergenic 35.0%
TTATTGTTGATACTGGACTC+AGG + Chr5:3752791-3752810 MsG0580024352.01.T01:intron 35.0%
TTGGATAATACGAGCACAAT+TGG - Chr5:3753065-3753084 None:intergenic 35.0%
! ACATAGCCTTGAAGTTTGAA+AGG - Chr5:3753503-3753522 None:intergenic 35.0%
! AGTTCTGTGTGTATTTTACG+CGG + Chr5:3752055-3752074 MsG0580024352.01.T01:intron 35.0%
! ATACTGAGAAAAGCGTGATT+AGG - Chr5:3751484-3751503 None:intergenic 35.0%
! ATCTATCTCTATTGATGGAC+TGG - Chr5:3752490-3752509 None:intergenic 35.0%
! ATGGAAAACTGAGGGTTTTA+GGG + Chr5:3753018-3753037 MsG0580024352.01.T01:intron 35.0%
! GGATTTGGAGCTTTATACAA+GGG + Chr5:3754289-3754308 MsG0580024352.01.T01:intron 35.0%
! TATGGAAAACTGAGGGTTTT+AGG + Chr5:3753017-3753036 MsG0580024352.01.T01:intron 35.0%
! TCTCTATTTGGATGATGATG+AGG + Chr5:3752019-3752038 MsG0580024352.01.T01:intron 35.0%
! TTCAAAGATGCACTGTGTTT+TGG + Chr5:3752536-3752555 MsG0580024352.01.T01:intron 35.0%
!! CTAGAGCATTGTATAAAGGA+TGG + Chr5:3753862-3753881 MsG0580024352.01.T01:CDS 35.0%
!! TCTGTTTCTGTATTTCCTAC+CGG + Chr5:3751740-3751759 MsG0580024352.01.T01:intron 35.0%
!!! GGTTTTAGGGGAATGTTTAA+AGG + Chr5:3753031-3753050 MsG0580024352.01.T01:intron 35.0%
AAACAGTGATCAGAAGGACA+TGG - Chr5:3753180-3753199 None:intergenic 40.0%
AAGGATTTCGGTACCATTTC+CGG - Chr5:3753243-3753262 None:intergenic 40.0%
AAGGGTAATCTTGAGTACAC+TGG + Chr5:3754229-3754248 MsG0580024352.01.T01:CDS 40.0%
AGCCTTGAAGTTTGAAAGGT+AGG - Chr5:3753499-3753518 None:intergenic 40.0%
ATGCTCTTTCAACAGTCTTC+CGG + Chr5:3753830-3753849 MsG0580024352.01.T01:CDS 40.0%
ATTCGCAGAAGAATGCAGAT+GGG + Chr5:3754166-3754185 MsG0580024352.01.T01:CDS 40.0%
CAGCTAACCTTGACTGAATT+GGG - Chr5:3754322-3754341 None:intergenic 40.0%
CAGTGGTATATGAAACTGCA+AGG + Chr5:3754487-3754506 MsG0580024352.01.T01:intron 40.0%
CTGCAAGGAAACAAAAACAC+GGG + Chr5:3754502-3754521 MsG0580024352.01.T01:intron 40.0%
CTTACAACTCCTATGACTGA+AGG - Chr5:3753891-3753910 None:intergenic 40.0%
CTTAGTACCCAATTCAGTCA+AGG + Chr5:3754312-3754331 MsG0580024352.01.T01:intron 40.0%
GCAGAATCTTCAATCTCTAG+AGG - Chr5:3752674-3752693 None:intergenic 40.0%
GGATTGAAGAGAACGGTATA+AGG - Chr5:3751617-3751636 None:intergenic 40.0%
TAATCTTGAGTACACTGGCA+TGG + Chr5:3754234-3754253 MsG0580024352.01.T01:CDS 40.0%
TCAGCTAACCTTGACTGAAT+TGG - Chr5:3754323-3754342 None:intergenic 40.0%
TCTTCTCTAATGGTTTCAGG+TGG + Chr5:3754638-3754657 MsG0580024352.01.T01:intron 40.0%
TCTTCTGCGAATGACATCAA+GGG - Chr5:3754158-3754177 None:intergenic 40.0%
TGCAAGGAAACAAAAACACG+GGG + Chr5:3754503-3754522 MsG0580024352.01.T01:intron 40.0%
TGGCGTCCAAATAACAACTT+TGG + Chr5:3752199-3752218 MsG0580024352.01.T01:intron 40.0%
TTCTTCTGCGAATGACATCA+AGG - Chr5:3754159-3754178 None:intergenic 40.0%
! AAAAGCGTGATTAGGAGGTT+TGG - Chr5:3751476-3751495 None:intergenic 40.0%
! GGGATTTGGAGCTTTATACA+AGG + Chr5:3754288-3754307 MsG0580024352.01.T01:intron 40.0%
! GTGCATCTTTGAAAATCAGC+TGG - Chr5:3752529-3752548 None:intergenic 40.0%
! TGAAAGTTTGCTCTTCAACC+TGG - Chr5:3752155-3752174 None:intergenic 40.0%
!! CTGTTTCTGTATTTCCTACC+GGG + Chr5:3751741-3751760 MsG0580024352.01.T01:intron 40.0%
!! TGGAAAACTGAGGGTTTTAG+GGG + Chr5:3753019-3753038 MsG0580024352.01.T01:intron 40.0%
!!! TTTTAGTTCACGAACAGCTG+TGG + Chr5:3752646-3752665 MsG0580024352.01.T01:intron 40.0%
AAAATGTGTGGATGGATGGC+AGG + Chr5:3752872-3752891 MsG0580024352.01.T01:intron 45.0%
AAATGTGTGGATGGATGGCA+GGG + Chr5:3752873-3752892 MsG0580024352.01.T01:intron 45.0%
AAGTGAAACTCACACTCCAC+CGG - Chr5:3751536-3751555 None:intergenic 45.0%
ATCTGCAAATCTCTTGTCGC+AGG + Chr5:3751502-3751521 MsG0580024352.01.T01:CDS 45.0%
CATTCGCAGAAGAATGCAGA+TGG + Chr5:3754165-3754184 MsG0580024352.01.T01:CDS 45.0%
CTTCTGCGAATGACATCAAG+GGG - Chr5:3754157-3754176 None:intergenic 45.0%
GAGATTGAAGATTCTGCTGC+AGG + Chr5:3752677-3752696 MsG0580024352.01.T01:CDS 45.0%
GAGTCTTGAGCAATTCCAAG+TGG - Chr5:3754065-3754084 None:intergenic 45.0%
GCATCTCCTCATCAATACAG+AGG + Chr5:3753799-3753818 MsG0580024352.01.T01:CDS 45.0%
GGATGGTTACCTTCAGTCAT+AGG + Chr5:3753879-3753898 MsG0580024352.01.T01:intron 45.0%
TCAGAGCTAAGTGTGACACA+AGG + Chr5:3754083-3754102 MsG0580024352.01.T01:CDS 45.0%
TTCAACAGTCTTCCGGAAGA+AGG + Chr5:3753837-3753856 MsG0580024352.01.T01:CDS 45.0%
TTCTGCGAATGACATCAAGG+GGG - Chr5:3754156-3754175 None:intergenic 45.0%
! AGTCTCTATCGGTTGCAAAC+CGG + Chr5:3753221-3753240 MsG0580024352.01.T01:intron 45.0%
! CTGAGAAAAGCGTGATTAGG+AGG - Chr5:3751481-3751500 None:intergenic 45.0%
! TATCGGTTGCAAACCGGAAA+TGG + Chr5:3753227-3753246 MsG0580024352.01.T01:intron 45.0%
! TGTATTTTACGCGGTGTCTC+TGG + Chr5:3752064-3752083 MsG0580024352.01.T01:intron 45.0%
!! GGCGCTAGAGCATTGTATAA+AGG + Chr5:3753858-3753877 MsG0580024352.01.T01:CDS 45.0%
!!! GACTGGTTTAGACGTGCTTT+TGG - Chr5:3752473-3752492 None:intergenic 45.0%
AATGCTCTAGCGCCTTCTTC+CGG - Chr5:3753852-3753871 None:intergenic 50.0%
AATGTCCGCAACTTACCCGA+TGG + Chr5:3753626-3753645 MsG0580024352.01.T01:CDS 50.0%
ACGAAGGCAATGGCTATGGA+AGG - Chr5:3754666-3754685 None:intergenic 50.0%
ATGGCGTCTCAAGATTCAGC+TGG + Chr5:3751403-3751422 MsG0580024352.01.T01:CDS 50.0%
CGAAGGCAATGGCTATGGAA+GGG - Chr5:3754665-3754684 None:intergenic 50.0%
CGAGATGGTCAAGACGTTCT+AGG + Chr5:3754692-3754711 MsG0580024352.01.T01:CDS 50.0%
CTCGTATGTCACGAAGGCAA+TGG - Chr5:3754676-3754695 None:intergenic 50.0%
GACCATCTCGTATGTCACGA+AGG - Chr5:3754682-3754701 None:intergenic 50.0%
GTGACACAAGGCTTGCATGT+GGG + Chr5:3754095-3754114 MsG0580024352.01.T01:CDS 50.0%
TATAATAAAGGCGGCCCGGT+AGG - Chr5:3751758-3751777 None:intergenic 50.0%
TGACACAAGGCTTGCATGTG+GGG + Chr5:3754096-3754115 MsG0580024352.01.T01:CDS 50.0%
TGCAAATCTCTTGTCGCAGG+TGG + Chr5:3751505-3751524 MsG0580024352.01.T01:CDS 50.0%
TGCCTTCGTGACATACGAGA+TGG + Chr5:3754677-3754696 MsG0580024352.01.T01:CDS 50.0%
TGGAACTATTGGCCAGACAG+TGG + Chr5:3754126-3754145 MsG0580024352.01.T01:CDS 50.0%
TGTCACGAAGGCAATGGCTA+TGG - Chr5:3754670-3754689 None:intergenic 50.0%
TGTCTGCAGATACCGTACGT+GGG + Chr5:3753988-3754007 MsG0580024352.01.T01:intron 50.0%
TGTGACACAAGGCTTGCATG+TGG + Chr5:3754094-3754113 MsG0580024352.01.T01:CDS 50.0%
TTGTCTGCAGATACCGTACG+TGG + Chr5:3753987-3754006 MsG0580024352.01.T01:intron 50.0%
! TCTAAAGTCTCCGCAATCGC+CGG + Chr5:3751442-3751461 MsG0580024352.01.T01:CDS 50.0%
AGGAGGTTTGGCACCTTCAC+CGG - Chr5:3751464-3751483 None:intergenic 55.0%
ATGCAGATGGGAGGCTGGAA+AGG + Chr5:3754178-3754197 MsG0580024352.01.T01:CDS 55.0%
CAGGTCCATCGGGTAAGTTG+CGG - Chr5:3753634-3753653 None:intergenic 55.0%
CGCAGAAGAATGCAGATGGG+AGG + Chr5:3754169-3754188 MsG0580024352.01.T01:CDS 55.0%
CTCACTCCTCTTCTGCGTCT+TGG + Chr5:3753576-3753595 MsG0580024352.01.T01:CDS 55.0%
CTCTTCTGCGTCTTGGAGCT+GGG + Chr5:3753583-3753602 MsG0580024352.01.T01:CDS 55.0%
CTTCCCGTCACCAGTGACAA+CGG - Chr5:3754212-3754231 None:intergenic 55.0%
CTTCCGTTGTCACTGGTGAC+GGG + Chr5:3754206-3754225 MsG0580024352.01.T01:CDS 55.0%
GAAGAATGCAGATGGGAGGC+TGG + Chr5:3754173-3754192 MsG0580024352.01.T01:CDS 55.0%
GCTTCCGTTGTCACTGGTGA+CGG + Chr5:3754205-3754224 MsG0580024352.01.T01:CDS 55.0%
TCTTCTGCGTCTTGGAGCTG+GGG + Chr5:3753584-3753603 MsG0580024352.01.T01:CDS 55.0%
!! GTTGTCACTGGTGACGGGAA+GGG + Chr5:3754211-3754230 MsG0580024352.01.T01:CDS 55.0%
CCAGCTCCAAGACGCAGAAG+AGG - Chr5:3753585-3753604 None:intergenic 60.0%
CCTCTTCTGCGTCTTGGAGC+TGG + Chr5:3753582-3753601 MsG0580024352.01.T01:CDS 60.0%
CGCAACTTACCCGATGGACC+TGG + Chr5:3753632-3753651 MsG0580024352.01.T01:CDS 60.0%
GCGAGGACGCCTAACTGTAC+AGG + Chr5:3753656-3753675 MsG0580024352.01.T01:CDS 60.0%
GGGGCTGCTGCTGGAACTAT+TGG + Chr5:3754115-3754134 MsG0580024352.01.T01:CDS 60.0%
! CAAGGGGGTATGCCACTGTC+TGG - Chr5:3754141-3754160 None:intergenic 60.0%
!! CGTTGTCACTGGTGACGGGA+AGG + Chr5:3754210-3754229 MsG0580024352.01.T01:CDS 60.0%
CAGTTAGGCGTCCTCGCACC+AGG - Chr5:3753653-3753672 None:intergenic 65.0%
CGTCCTCGCACCAGGTCCAT+CGG - Chr5:3753645-3753664 None:intergenic 65.0%
CTTGCATGTGGGGCTGCTGC+TGG + Chr5:3754106-3754125 MsG0580024352.01.T01:CDS 65.0%
CTTGGAGCTGGGGCATGTGC+AGG + Chr5:3753594-3753613 MsG0580024352.01.T01:CDS 65.0%
CTTGTCGCAGGTGGAGTCGC+CGG + Chr5:3751514-3751533 MsG0580024352.01.T01:CDS 65.0%
GGCACCTTCACCGGCGATTG+CGG - Chr5:3751455-3751474 None:intergenic 65.0%
GGCGCTGCTTCCGTTGTCAC+TGG + Chr5:3754199-3754218 MsG0580024352.01.T01:CDS 65.0%
GTCCTCGCACCAGGTCCATC+GGG - Chr5:3753644-3753663 None:intergenic 65.0%
GTCTCCGCAATCGCCGGTGA+AGG + Chr5:3751448-3751467 MsG0580024352.01.T01:CDS 65.0%
TACCCGATGGACCTGGTGCG+AGG + Chr5:3753639-3753658 MsG0580024352.01.T01:CDS 65.0%
GTCGCAGGTGGAGTCGCCGG+TGG + Chr5:3751517-3751536 MsG0580024352.01.T01:CDS 75.0%
Chromosome Type Strat End Strand Name
Chr5 gene 3751391 3754947 3751391 ID=MsG0580024352.01;Name=MsG0580024352.01
Chr5 mRNA 3751391 3754947 3751391 ID=MsG0580024352.01.T01;Parent=MsG0580024352.01;Name=MsG0580024352.01.T01;_AED=0.36;_eAED=0.38;_QI=0|0.2|0.16|1|0.6|0.5|6|208|274
Chr5 exon 3751391 3751542 3751391 ID=MsG0580024352.01.T01:exon:1181;Parent=MsG0580024352.01.T01
Chr5 exon 3752652 3752718 3752652 ID=MsG0580024352.01.T01:exon:1182;Parent=MsG0580024352.01.T01
Chr5 exon 3753444 3753677 3753444 ID=MsG0580024352.01.T01:exon:1183;Parent=MsG0580024352.01.T01
Chr5 exon 3753793 3753893 3753793 ID=MsG0580024352.01.T01:exon:1184;Parent=MsG0580024352.01.T01
Chr5 exon 3754081 3754268 3754081 ID=MsG0580024352.01.T01:exon:1185;Parent=MsG0580024352.01.T01
Chr5 exon 3754657 3754947 3754657 ID=MsG0580024352.01.T01:exon:1186;Parent=MsG0580024352.01.T01
Chr5 CDS 3751391 3751542 3751391 ID=MsG0580024352.01.T01:cds;Parent=MsG0580024352.01.T01
Chr5 CDS 3752652 3752718 3752652 ID=MsG0580024352.01.T01:cds;Parent=MsG0580024352.01.T01
Chr5 CDS 3753444 3753677 3753444 ID=MsG0580024352.01.T01:cds;Parent=MsG0580024352.01.T01
Chr5 CDS 3753793 3753893 3753793 ID=MsG0580024352.01.T01:cds;Parent=MsG0580024352.01.T01
Chr5 CDS 3754081 3754268 3754081 ID=MsG0580024352.01.T01:cds;Parent=MsG0580024352.01.T01
Chr5 CDS 3754657 3754739 3754657 ID=MsG0580024352.01.T01:cds;Parent=MsG0580024352.01.T01
Chr5 three_prime_UTR 3754740 3754947 3754740 ID=MsG0580024352.01.T01:three_prime_utr;Parent=MsG0580024352.01.T01
Gene Sequence

>MsG0580024352.01.T01

CTTCAAGCTTCAATGGCGTCTCAAGATTCAGCTGGAAAGACAAATGATCAGTCTAAAGTCTCCGCAATCGCCGGTGAAGGTGCCAAACCTCCTAATCACGCTTTTCTCAGTATCTGCAAATCTCTTGTCGCAGGTGGAGTCGCCGGTGGAGTTTCACGAACAGCTGTGGCTCCTCTAGAGATTGAAGATTCTGCTGCAGGTAGTTCCCTTAAGCTTATACTCGATGTATCCTTCAATCTGCATTTTTATTCATATTATGTGAGGGGTTTTACCTACCTTTCAAACTTCAAGGCTATGTCCATTGTCACACTTTATTTTTATTTTTATTTTTATTTTGCAGAAGAAGCTCAACTCACTCCTCTTCTGCGTCTTGGAGCTGGGGCATGTGCAGGAATCATTGCAATGTCCGCAACTTACCCGATGGACCTGGTGCGAGGACGCCTAACTGTACAGACAGAAGCATCTCCTCATCAATACAGAGGAATTTTTAATGCTCTTTCAACAGTCTTCCGGAAGAAGGCGCTAGAGCATTGTATAAAGGATGGTTACCTTCAACTCAGAGCTAAGTGTGACACAAGGCTTGCATGTGGGGCTGCTGCTGGAACTATTGGCCAGACAGTGGCATACCCCCTTGATGTCATTCGCAGAAGAATGCAGATGGGAGGCTGGAAAGGCGCTGCTTCCGTTGTCACTGGTGACGGGAAGGGTAATCTTGAGTACACTGGCATGGTTGATGCATTCAGTGGTCCCTTCCATAGCCATTGCCTTCGTGACATACGAGATGGTCAAGACGTTCTAGGAGTTGAAATGAGAATATCTGACTGA
Protein sequence

>MsG0580024352.01.T01

LQASMASQDSAGKTNDQSKVSAIAGEGAKPPNHAFLSICKSLVAGGVAGGVSRTAVAPLEIEDSAAGSSLKLILDVSFNLHFYSYYVRGFTYLSNFKAMSIVTLYFYFYFYFAEEAQLTPLLRLGAGACAGIIAMSATYPMDLVRGRLTVQTEASPHQYRGIFNALSTVFRKKALEHCIKDGYLQLRAKCDTRLACGAAAGTIGQTVAYPLDVIRRRMQMGGWKGAASVVTGDGKGNLEYTGMVDAFSGPFHSHCLRDIRDGQDVLGVEMRISD*