Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024410.01.T01 | XP_024640146.1 | 98.592 | 71 | 1 | 0 | 1 | 71 | 57 | 127 | 8.68E-40 | 145 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024410.01.T01 | Q93Z92 | 69.863 | 73 | 22 | 0 | 1 | 73 | 81 | 153 | 2.14E-32 | 119 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024410.01.T01 | G7KDG0 | 98.592 | 71 | 1 | 0 | 1 | 71 | 57 | 127 | 4.33e-40 | 145 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0280010884.01 | MsG0580024410.01 | 0.813927 | 1.998100e-51 | 1.322199e-48 |
| MsG0580024410.01 | MsG0880045704.01 | 0.824177 | 9.294841e-54 | 8.171963e-51 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024410.01.T01 | MTR_5g010720 | 98.592 | 71 | 1 | 0 | 1 | 71 | 57 | 127 | 1.10e-43 | 145 |
| MsG0580024410.01.T01 | MTR_4g102870 | 80.282 | 71 | 14 | 0 | 1 | 71 | 45 | 115 | 3.87e-35 | 123 |
| MsG0580024410.01.T01 | MTR_1g032440 | 61.972 | 71 | 27 | 0 | 2 | 72 | 41 | 111 | 1.74e-26 | 99.4 |
| MsG0580024410.01.T01 | MTR_1g032440 | 61.972 | 71 | 27 | 0 | 2 | 72 | 41 | 111 | 2.75e-26 | 99.4 |
| MsG0580024410.01.T01 | MTR_8g105330 | 60.563 | 71 | 28 | 0 | 2 | 72 | 30 | 100 | 4.46e-25 | 95.9 |
| MsG0580024410.01.T01 | MTR_7g076010 | 60.000 | 70 | 28 | 0 | 1 | 70 | 50 | 119 | 1.39e-18 | 79.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024410.01.T01 | AT4G11680 | 69.863 | 73 | 22 | 0 | 1 | 73 | 81 | 153 | 2.18e-33 | 119 |
| MsG0580024410.01.T01 | AT1G12760 | 74.648 | 71 | 18 | 0 | 1 | 71 | 1 | 71 | 2.84e-33 | 117 |
| MsG0580024410.01.T01 | AT4G11680 | 69.863 | 73 | 22 | 0 | 1 | 73 | 81 | 153 | 3.99e-33 | 118 |
| MsG0580024410.01.T01 | AT1G12760 | 72.603 | 73 | 20 | 0 | 1 | 73 | 72 | 144 | 1.21e-31 | 114 |
| MsG0580024410.01.T01 | AT3G61180 | 67.123 | 73 | 24 | 0 | 1 | 73 | 56 | 128 | 5.18e-29 | 107 |
| MsG0580024410.01.T01 | AT1G63170 | 74.648 | 71 | 18 | 0 | 1 | 71 | 46 | 116 | 7.58e-27 | 101 |
| MsG0580024410.01.T01 | AT1G68070 | 53.086 | 81 | 34 | 1 | 2 | 78 | 41 | 121 | 3.33e-24 | 94.0 |
| MsG0580024410.01.T01 | AT2G01735 | 54.167 | 72 | 33 | 0 | 2 | 73 | 50 | 121 | 5.09e-21 | 85.5 |
| MsG0580024410.01.T01 | AT2G01735 | 54.167 | 72 | 33 | 0 | 2 | 73 | 50 | 121 | 2.03e-20 | 82.4 |
Find 24 sgRNAs with CRISPR-Local
Find 27 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CACAGCCTCAACGGCATTTC+CGG | 0.201324 | 5:+4494234 | None:intergenic |
| TGTTGCAATGTTTCTTCATA+TGG | 0.332904 | 5:-4494197 | MsG0580024410.01.T01:CDS |
| GCATTTCCGGAGCTTCATTC+CGG | 0.387248 | 5:+4494247 | None:intergenic |
| TTGTGATTCTCGATATCGTT+TGG | 0.398024 | 5:-4494314 | MsG0580024410.01.T01:CDS |
| CCGTTGAGGCTGTGGATTGC+GGG | 0.401372 | 5:-4494225 | MsG0580024410.01.T01:CDS |
| CTTCGTCATTGTGGCGGGAA+CGG | 0.437914 | 5:-4494283 | MsG0580024410.01.T01:CDS |
| CGGAAATGCCGTTGAGGCTG+TGG | 0.443205 | 5:-4494233 | MsG0580024410.01.T01:CDS |
| TAGAAGAACGACAAAGCGAT+TGG | 0.486101 | 5:-4494353 | MsG0580024410.01.T01:CDS |
| TTCGCCTTCGTCATTGTGGC+GGG | 0.487248 | 5:-4494288 | MsG0580024410.01.T01:CDS |
| TTTGAGCCGGAATGAAGCTC+CGG | 0.515420 | 5:-4494253 | MsG0580024410.01.T01:CDS |
| AGAAGAACGACAAAGCGATT+GGG | 0.524313 | 5:-4494352 | MsG0580024410.01.T01:CDS |
| TTGAGTATAGGAGAAGGAGA+AGG | 0.554902 | 5:-4494162 | MsG0580024410.01.T01:CDS |
| CGATTGGGCGTATTCGAAGC+CGG | 0.557403 | 5:-4494337 | MsG0580024410.01.T01:CDS |
| CCCGCAATCCACAGCCTCAA+CGG | 0.557587 | 5:+4494225 | None:intergenic |
| CTTCGCCTTCGTCATTGTGG+CGG | 0.571163 | 5:-4494289 | MsG0580024410.01.T01:CDS |
| ACGATATCGAGAATCACAAC+CGG | 0.580305 | 5:+4494318 | None:intergenic |
| GCCGTTGAGGCTGTGGATTG+CGG | 0.586081 | 5:-4494226 | MsG0580024410.01.T01:CDS |
| GAACTTCGCCTTCGTCATTG+TGG | 0.588422 | 5:-4494292 | MsG0580024410.01.T01:CDS |
| ACGTTCTTCGTCATCGAATG+CGG | 0.597603 | 5:-4494134 | MsG0580024410.01.T01:CDS |
| GTTTCGCGAACAACGACGGA+AGG | 0.617701 | 5:+4494390 | None:intergenic |
| GGCAGTTTCGCGAACAACGA+CGG | 0.621073 | 5:+4494386 | None:intergenic |
| AAGCTCCGGAAATGCCGTTG+AGG | 0.640748 | 5:-4494239 | MsG0580024410.01.T01:CDS |
| CGTTCCCGCCACAATGACGA+AGG | 0.662267 | 5:+4494284 | None:intergenic |
| TCGTTCTTCTAATTGCTCTG+CGG | 0.681822 | 5:+4494365 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTGTGTTTGTTTTGAGTAT+AGG | - | Chr5:4494356-4494375 | MsG0580024410.01.T01:CDS | 25.0% |
| TGTTGCAATGTTTCTTCATA+TGG | - | Chr5:4494333-4494352 | MsG0580024410.01.T01:CDS | 30.0% | |
| !!! | TTTGTTTTGAGTATAGGAGA+AGG | - | Chr5:4494362-4494381 | MsG0580024410.01.T01:CDS | 30.0% |
| TTGTGATTCTCGATATCGTT+TGG | - | Chr5:4494216-4494235 | MsG0580024410.01.T01:CDS | 35.0% | |
| ACGATATCGAGAATCACAAC+CGG | + | Chr5:4494215-4494234 | None:intergenic | 40.0% | |
| AGAAGAACGACAAAGCGATT+GGG | - | Chr5:4494178-4494197 | MsG0580024410.01.T01:CDS | 40.0% | |
| TAGAAGAACGACAAAGCGAT+TGG | - | Chr5:4494177-4494196 | MsG0580024410.01.T01:CDS | 40.0% | |
| TCGTTCTTCTAATTGCTCTG+CGG | + | Chr5:4494168-4494187 | None:intergenic | 40.0% | |
| TTGAGTATAGGAGAAGGAGA+AGG | - | Chr5:4494368-4494387 | MsG0580024410.01.T01:CDS | 40.0% | |
| ACGTTCTTCGTCATCGAATG+CGG | - | Chr5:4494396-4494415 | MsG0580024410.01.T01:CDS | 45.0% | |
| GAACTTCGCCTTCGTCATTG+TGG | - | Chr5:4494238-4494257 | MsG0580024410.01.T01:CDS | 50.0% | |
| GCATTTCCGGAGCTTCATTC+CGG | + | Chr5:4494286-4494305 | None:intergenic | 50.0% | |
| TTTGAGCCGGAATGAAGCTC+CGG | - | Chr5:4494277-4494296 | MsG0580024410.01.T01:CDS | 50.0% | |
| !!! | GAACGGCGTTGTTTTTGAGC+CGG | - | Chr5:4494264-4494283 | MsG0580024410.01.T01:CDS | 50.0% |
| AAGCTCCGGAAATGCCGTTG+AGG | - | Chr5:4494291-4494310 | MsG0580024410.01.T01:CDS | 55.0% | |
| CACAGCCTCAACGGCATTTC+CGG | + | Chr5:4494299-4494318 | None:intergenic | 55.0% | |
| CGATTGGGCGTATTCGAAGC+CGG | - | Chr5:4494193-4494212 | MsG0580024410.01.T01:CDS | 55.0% | |
| CTTCGCCTTCGTCATTGTGG+CGG | - | Chr5:4494241-4494260 | MsG0580024410.01.T01:CDS | 55.0% | |
| GGCAGTTTCGCGAACAACGA+CGG | + | Chr5:4494147-4494166 | None:intergenic | 55.0% | |
| GTTTCGCGAACAACGACGGA+AGG | + | Chr5:4494143-4494162 | None:intergenic | 55.0% | |
| TTCGCCTTCGTCATTGTGGC+GGG | - | Chr5:4494242-4494261 | MsG0580024410.01.T01:CDS | 55.0% | |
| ! | CTTCGTCATTGTGGCGGGAA+CGG | - | Chr5:4494247-4494266 | MsG0580024410.01.T01:CDS | 55.0% |
| CCCGCAATCCACAGCCTCAA+CGG | + | Chr5:4494308-4494327 | None:intergenic | 60.0% | |
| CGGAAATGCCGTTGAGGCTG+TGG | - | Chr5:4494297-4494316 | MsG0580024410.01.T01:CDS | 60.0% | |
| CGTTCCCGCCACAATGACGA+AGG | + | Chr5:4494249-4494268 | None:intergenic | 60.0% | |
| ! | CCGTTGAGGCTGTGGATTGC+GGG | - | Chr5:4494305-4494324 | MsG0580024410.01.T01:CDS | 60.0% |
| ! | GCCGTTGAGGCTGTGGATTG+CGG | - | Chr5:4494304-4494323 | MsG0580024410.01.T01:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 4494131 | 4494421 | 4494131 | ID=MsG0580024410.01;Name=MsG0580024410.01 |
| Chr5 | mRNA | 4494131 | 4494421 | 4494131 | ID=MsG0580024410.01.T01;Parent=MsG0580024410.01;Name=MsG0580024410.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|96 |
| Chr5 | exon | 4494131 | 4494421 | 4494131 | ID=MsG0580024410.01.T01:exon:1394;Parent=MsG0580024410.01.T01 |
| Chr5 | CDS | 4494131 | 4494421 | 4494131 | ID=MsG0580024410.01.T01:cds;Parent=MsG0580024410.01.T01 |
| Gene Sequence |
| Protein sequence |
