Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024422.01.T01 | XP_039689975.1 | 80 | 55 | 8 | 1 | 16 | 67 | 114 | 168 | 3.54E-21 | 91.3 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024422.01.T01 | Q56XX3 | 64.706 | 51 | 13 | 2 | 16 | 61 | 266 | 316 | 7.13E-13 | 64.3 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024422.01.T01 | A0A396GU09 | 92.857 | 42 | 3 | 0 | 16 | 57 | 311 | 352 | 7.44e-19 | 88.6 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000091.01 | MsG0580024422.01 | 0.816926 | 4.297648e-52 | 3.086288e-49 |
| MsG0180004465.01 | MsG0580024422.01 | 0.819501 | 1.123566e-52 | 8.661701e-50 |
| MsG0180004926.01 | MsG0580024422.01 | 0.811105 | 8.267214e-51 | 5.072752e-48 |
| MsG0280006309.01 | MsG0580024422.01 | 0.800983 | 1.115667e-48 | 5.267506e-46 |
| MsG0280010722.01 | MsG0580024422.01 | 0.834059 | 3.731419e-56 | 4.377921e-53 |
| MsG0380013363.01 | MsG0580024422.01 | 0.818036 | 2.417277e-52 | 1.789402e-49 |
| MsG0380014701.01 | MsG0580024422.01 | 0.806741 | 7.098410e-50 | 3.882884e-47 |
| MsG0480019035.01 | MsG0580024422.01 | 0.811473 | 6.879534e-51 | 4.262523e-48 |
| MsG0480023045.01 | MsG0580024422.01 | 0.810657 | 1.033654e-50 | 6.267317e-48 |
| MsG0580024038.01 | MsG0580024422.01 | 0.800289 | 1.545591e-48 | 7.169686e-46 |
| MsG0580024422.01 | MsG0880045372.01 | 0.802910 | 4.483061e-49 | 2.222633e-46 |
| MsG0380016387.01 | MsG0580024422.01 | 0.808551 | 2.930093e-50 | 1.680312e-47 |
| MsG0380016391.01 | MsG0580024422.01 | 0.808047 | 3.753039e-50 | 2.123854e-47 |
| MsG0280007072.01 | MsG0580024422.01 | 0.807236 | 5.579043e-50 | 3.091164e-47 |
| MsG0280007955.01 | MsG0580024422.01 | 0.806208 | 9.198915e-50 | 4.963955e-47 |
| MsG0280010517.01 | MsG0580024422.01 | 0.818474 | 1.923363e-52 | 1.441219e-49 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024422.01.T01 | MTR_5g010860 | 77.551 | 49 | 8 | 1 | 16 | 61 | 109 | 157 | 7.21e-20 | 78.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024422.01.T01 | AT2G02148 | 64.706 | 51 | 13 | 2 | 16 | 61 | 266 | 316 | 7.27e-14 | 64.3 |
| MsG0580024422.01.T01 | AT2G02148 | 64.706 | 51 | 13 | 2 | 16 | 61 | 266 | 316 | 7.27e-14 | 64.3 |
| MsG0580024422.01.T01 | AT2G02148 | 64.706 | 51 | 13 | 2 | 16 | 61 | 266 | 316 | 7.27e-14 | 64.3 |
| MsG0580024422.01.T01 | AT2G02148 | 64.706 | 51 | 13 | 2 | 16 | 61 | 266 | 316 | 7.27e-14 | 64.3 |
| MsG0580024422.01.T01 | AT2G02148 | 64.706 | 51 | 13 | 2 | 16 | 61 | 266 | 316 | 9.84e-14 | 63.9 |
Find 13 sgRNAs with CRISPR-Local
Find 23 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGCATGATGTTGCCTCATTT+TGG | 0.197106 | 5:+4736920 | MsG0580024422.01.T01:CDS |
| ATCATGCTTAAAGGGATATC+TGG | 0.221086 | 5:-4736905 | None:intergenic |
| AAAGGGATATCTGGGAAATT+TGG | 0.243233 | 5:-4736896 | None:intergenic |
| TGAGGCAACATCATGCTTAA+AGG | 0.260855 | 5:-4736914 | None:intergenic |
| GAATACGAGCACTATTTCTT+AGG | 0.335785 | 5:-4736502 | None:intergenic |
| GGTTTAAAAGATTGACAGGT+TGG | 0.403575 | 5:+4736792 | MsG0580024422.01.T01:intron |
| GAGGCAACATCATGCTTAAA+GGG | 0.411665 | 5:-4736913 | None:intergenic |
| TATAGGTTTAAAAGATTGAC+AGG | 0.420118 | 5:+4736788 | MsG0580024422.01.T01:intron |
| CCACGCCTATGCTTGTGTCT+TGG | 0.506145 | 5:-4736857 | None:intergenic |
| TCATGCTTAAAGGGATATCT+GGG | 0.515190 | 5:-4736904 | None:intergenic |
| GTATTCTATTTGTAGTTCTG+TGG | 0.532224 | 5:+4736519 | MsG0580024422.01.T01:CDS |
| CCAAGACACAAGCATAGGCG+TGG | 0.662445 | 5:+4736857 | MsG0580024422.01.T01:CDS |
| ACTCTCCAAGACACAAGCAT+AGG | 0.666512 | 5:+4736852 | MsG0580024422.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATGAGTGTAATAACAAAAT+CGG | + | Chr5:4736685-4736704 | MsG0580024422.01.T01:intron | 20.0% |
| !! | TTCGTTAAAATGTAACAAAA+GGG | - | Chr5:4736642-4736661 | None:intergenic | 20.0% |
| ! | AATAACAAAATCGGTTATGA+TGG | + | Chr5:4736694-4736713 | MsG0580024422.01.T01:intron | 25.0% |
| ! | ATAACAAAATCGGTTATGAT+GGG | + | Chr5:4736695-4736714 | MsG0580024422.01.T01:intron | 25.0% |
| ! | CTTCGTTAAAATGTAACAAA+AGG | - | Chr5:4736643-4736662 | None:intergenic | 25.0% |
| ! | TATAGGTTTAAAAGATTGAC+AGG | + | Chr5:4736788-4736807 | MsG0580024422.01.T01:intron | 25.0% |
| !! | CATTTCATTTGAAATTTGCA+AGG | + | Chr5:4736730-4736749 | MsG0580024422.01.T01:intron | 25.0% |
| !!! | CTTGCTTTGTTTTTGTTTAT+AGG | + | Chr5:4736771-4736790 | MsG0580024422.01.T01:intron | 25.0% |
| !!! | CTTTTGTTACATTTTAACGA+AGG | + | Chr5:4736641-4736660 | MsG0580024422.01.T01:intron | 25.0% |
| AAAATGTAACAAAAGGGAAC+AGG | - | Chr5:4736636-4736655 | None:intergenic | 30.0% | |
| ! | GTATTCTATTTGTAGTTCTG+TGG | + | Chr5:4736519-4736538 | MsG0580024422.01.T01:CDS | 30.0% |
| AAACAAAGCAAGTGAGACTA+AGG | - | Chr5:4736763-4736782 | None:intergenic | 35.0% | |
| AAAGGGATATCTGGGAAATT+TGG | - | Chr5:4736899-4736918 | None:intergenic | 35.0% | |
| ATCATGCTTAAAGGGATATC+TGG | - | Chr5:4736908-4736927 | None:intergenic | 35.0% | |
| GGTTTAAAAGATTGACAGGT+TGG | + | Chr5:4736792-4736811 | MsG0580024422.01.T01:intron | 35.0% | |
| TCATGCTTAAAGGGATATCT+GGG | - | Chr5:4736907-4736926 | None:intergenic | 35.0% | |
| ! | GAATACGAGCACTATTTCTT+AGG | - | Chr5:4736505-4736524 | None:intergenic | 35.0% |
| GAGGCAACATCATGCTTAAA+GGG | - | Chr5:4736916-4736935 | None:intergenic | 40.0% | |
| TGAGGCAACATCATGCTTAA+AGG | - | Chr5:4736917-4736936 | None:intergenic | 40.0% | |
| ! | AGCATGATGTTGCCTCATTT+TGG | + | Chr5:4736920-4736939 | MsG0580024422.01.T01:CDS | 40.0% |
| ACTCTCCAAGACACAAGCAT+AGG | + | Chr5:4736852-4736871 | MsG0580024422.01.T01:CDS | 45.0% | |
| CCAAGACACAAGCATAGGCG+TGG | + | Chr5:4736857-4736876 | MsG0580024422.01.T01:CDS | 55.0% | |
| CCACGCCTATGCTTGTGTCT+TGG | - | Chr5:4736860-4736879 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 4736494 | 4736949 | 4736494 | ID=MsG0580024422.01;Name=MsG0580024422.01 |
| Chr5 | mRNA | 4736494 | 4736949 | 4736494 | ID=MsG0580024422.01.T01;Parent=MsG0580024422.01;Name=MsG0580024422.01.T01;_AED=0.66;_eAED=0.66;_QI=0|0|0|0.5|0|0|2|0|67 |
| Chr5 | exon | 4736494 | 4736540 | 4736494 | ID=MsG0580024422.01.T01:exon:18980;Parent=MsG0580024422.01.T01 |
| Chr5 | exon | 4736793 | 4736949 | 4736793 | ID=MsG0580024422.01.T01:exon:18981;Parent=MsG0580024422.01.T01 |
| Chr5 | CDS | 4736494 | 4736540 | 4736494 | ID=MsG0580024422.01.T01:cds;Parent=MsG0580024422.01.T01 |
| Chr5 | CDS | 4736793 | 4736949 | 4736793 | ID=MsG0580024422.01.T01:cds;Parent=MsG0580024422.01.T01 |
| Gene Sequence |
| Protein sequence |
