Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014701.01.T01 | XP_013460146.1 | 95.39 | 564 | 22 | 2 | 1 | 564 | 1 | 560 | 0 | 992 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014701.01.T01 | A0A072UX49 | 95.390 | 564 | 22 | 2 | 1 | 564 | 1 | 560 | 0.0 | 992 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080047869.01 | MsG0380014701.01 | 0.814015 | 1.909902e-51 | 1.266933e-48 |
| MsG0080047944.01 | MsG0380014701.01 | 0.822405 | 2.410355e-53 | 2.015750e-50 |
| MsG0080047957.01 | MsG0380014701.01 | 0.831148 | 1.967595e-55 | 2.116844e-52 |
| MsG0080047959.01 | MsG0380014701.01 | 0.813848 | 2.079568e-51 | 1.373196e-48 |
| MsG0080048404.01 | MsG0380014701.01 | 0.815748 | 7.886921e-52 | 5.484371e-49 |
| MsG0080048461.01 | MsG0380014701.01 | 0.891462 | 3.887579e-74 | 3.411806e-70 |
| MsG0080048462.01 | MsG0380014701.01 | 0.871316 | 7.497060e-67 | 3.011349e-63 |
| MsG0080048672.01 | MsG0380014701.01 | 0.817920 | 2.567978e-52 | 1.895027e-49 |
| MsG0080048769.01 | MsG0380014701.01 | 0.832624 | 8.498350e-56 | 9.551683e-53 |
| MsG0080048787.01 | MsG0380014701.01 | 0.832824 | 7.581348e-56 | 8.571685e-53 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014701.01.T01 | MTR_3g462680 | 95.390 | 564 | 22 | 2 | 1 | 564 | 1 | 560 | 0.0 | 992 |
| MsG0380014701.01.T01 | MTR_7g066730 | 35.385 | 520 | 223 | 23 | 139 | 564 | 243 | 743 | 1.45e-54 | 197 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014701.01.T01 | AT5G58930 | 35.117 | 598 | 238 | 19 | 9 | 551 | 15 | 517 | 7.87e-73 | 242 |
| MsG0380014701.01.T01 | AT3G46990 | 35.135 | 592 | 266 | 22 | 16 | 555 | 22 | 547 | 1.92e-62 | 215 |
| MsG0380014701.01.T01 | AT3G09070 | 29.582 | 693 | 265 | 29 | 9 | 545 | 29 | 654 | 3.15e-51 | 187 |
| MsG0380014701.01.T01 | AT2G38070 | 31.327 | 648 | 267 | 28 | 9 | 558 | 36 | 603 | 3.94e-50 | 183 |
| MsG0380014701.01.T01 | AT5G01170 | 28.268 | 612 | 260 | 25 | 3 | 541 | 20 | 525 | 3.86e-32 | 130 |
Find 175 sgRNAs with CRISPR-Local
Find 185 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAACCCTCGAAGGAAATCGG+CGG | 0.720310 | 3:+58659650 | MsG0380014701.01.T01:CDS |
| AAACCGGTGATGGGTTGGGA+AGG | 0.521249 | 3:-58658697 | None:intergenic |
| AAAGGAAGATAAATTGGGAG+AGG | 0.549670 | 3:+58659950 | MsG0380014701.01.T01:CDS |
| AACAATGTCGATTGCGACGG+TGG | 0.555798 | 3:+58659225 | MsG0380014701.01.T01:CDS |
| AACCGGTGATGGGTTGGGAA+GGG | 0.573380 | 3:-58658696 | None:intergenic |
| AACGAACAAGTTTCACAAAT+GGG | 0.429380 | 3:+58659890 | MsG0380014701.01.T01:CDS |
| AAGAAGCTTGGTCACCGGCG+CGG | 0.680438 | 3:-58658816 | None:intergenic |
| AAGAGAAAGGAAGATAAATT+GGG | 0.436925 | 3:+58659945 | MsG0380014701.01.T01:CDS |
| AAGAGTGATATCTAATGCCA+AGG | 0.582042 | 3:+58659695 | MsG0380014701.01.T01:CDS |
| AAGTTGAGGAGGGTGGTTAA+TGG | 0.360472 | 3:+58660026 | MsG0380014701.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AACGAACAAGTTTCACAAAT+GGG | + | Chr3:58659890-58659909 | MsG0380014701.01.T01:CDS | 30.0% | |
| ATAGATCTTGAAATTCGTAG+CGG | + | Chr3:58659099-58659118 | MsG0380014701.01.T01:CDS | 30.0% | |
| GAAGAGAAAGGAAGATAAAT+TGG | + | Chr3:58659944-58659963 | MsG0380014701.01.T01:CDS | 30.0% | |
| GGAGAATATGGTTTATCATT+GGG | + | Chr3:58659336-58659355 | MsG0380014701.01.T01:CDS | 30.0% | |
| TCATCATTTATAGCAACCAT+TGG | - | Chr3:58659480-58659499 | None:intergenic | 30.0% | |
| TGGAGAATATGGTTTATCAT+TGG | + | Chr3:58659335-58659354 | MsG0380014701.01.T01:CDS | 30.0% | |
| TTCAATCTGATTGTAGAACA+GGG | + | Chr3:58659817-58659836 | MsG0380014701.01.T01:CDS | 30.0% | |
| AAAAAGCAGAAGAAGAAACC+TGG | + | Chr3:58658940-58658959 | MsG0380014701.01.T01:CDS | 35.0% | |
| AAAAATCATAGTAGAACGGC+TGG | + | Chr3:58660104-58660123 | MsG0380014701.01.T01:CDS | 35.0% | |
| AAAGGAAGATAAATTGGGAG+AGG | + | Chr3:58659950-58659969 | MsG0380014701.01.T01:CDS | 35.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 58658655 | 58660349 | 58658655 | ID=MsG0380014701.01;Name=MsG0380014701.01 |
| Chr3 | mRNA | 58658655 | 58660349 | 58658655 | ID=MsG0380014701.01.T01;Parent=MsG0380014701.01;Name=MsG0380014701.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|564 |
| Chr3 | exon | 58658655 | 58660349 | 58658655 | ID=MsG0380014701.01.T01:exon:18924;Parent=MsG0380014701.01.T01 |
| Chr3 | CDS | 58658655 | 58660349 | 58658655 | ID=MsG0380014701.01.T01:cds;Parent=MsG0380014701.01.T01 |
| Gene Sequence |
>MsG0380014701.01.T01 ATGACTTCCAAAACGCGTCGTTTAACCACCTGTCACCGTCACCCTTCCCAACCCATCACCGGTTTCTGCGCTTCCTGCCTCACCGAACGTCTCTCCGGCATCGAATCCTCCTCCGCCGACGCCGAACCCGAACTCCGCCGTACCAAATCTTGCTCCGGCAGCCGCGCCGGTGACCAAGCTTCTTCCTCCGCCGTCGAGCCTCGTCGTAGGTCGTGTGAGATCCTCGCTCCACCGGAGCAGAACCGTGGCACGCTGTCTGACCTGTTTAACATCGACGACGAGAAGAAAAAGCAGAAGAAGAAACCTGGTTTGAATCGGTGTCTCGAAAACGAATCTCGAAAATCAGGTTTTGAGATTATCGATTTCGACGGTGGTGGTGACATTGTTAGGGTTTTAGGTGAAGATGAAAATGATGAAGAAGAGACGAAGACGATGAAGGAGTTTATAGATCTTGAAATTCGTAGCGGGAAGAATGCAGGGAGAGATTCTTCTAGAACGAGTTTCTGGGATGCGGCTTCGGTTTTCAGCAAGAGGTTGAGGAAATGGAAACGGAAGCAGAAGTTGAAGAGAAACAATGTCGATTGCGACGGTGGCGGTGTTACCGGAAATGGCGGTGGTTTGAGGAGTATGGAGGTTGAGAAGCAAAGGAGGAGAAATTTGAGAGAGACGCTATCTGAGGTTGGAGAATATGGTTTATCATTGGGTAGAAGATCGTGTGATACTGATCCTAGGTTATCCGTGGATGATTCGAGGTTTTCATTCGACGCGCCTCGTGCTTCTTGGGATGGTTATTTGATTGGGAAAACTTGTTCGAGGTTTTCGCCAATGGTTGCTATAAATGATGATAGAGTTTTGGTTGAAGAAGAAGAAGATGAAGAAGGAGAAGAAGAGGGGGTGGTGAATTCGGAAAGTAGTGTTGGTGGACTATATCCTGGTGATTCAGCTGAAACAAAGCATTACTATTCGGATCGGAGGAGGAGGAGTTTCGATAGGTCAAACCCTCGAAGGAAATCGGCGGTGATTGGAGATGTTGATGAATTAAGAGTGATATCTAATGCCAAGGTTTCTCCGGCTACAACCGAGTTATTCTACGGTGCAAAGGTTTTGATCACTGAGGAAGATTTGAGGGATGCTAATTTGAAACCGAAGAATAGTGTTAATGTTCAATCTGATTGTAGAACAGGGTCTGCTTCTAAGGAAGCTTGTGATGTTGAAATTGGAAATGATCAAAAGGGAACGAACAAGTTTCACAAATGGGGCAAGTTGTGGAATAAGTTGGGGTTAGTACAGAAGAGAAAGGAAGATAAATTGGGAGAGGAAGAATGTGTTGCTGGTGATGTTGTTAATAAGCCGATTGTAGAGTCTTGGCAGAAGTTGAGGAGGGTGGTTAATGGACAAGGGAGTGAATCGGTTAGCCAGAAGCTTATTCGTAGCTATAGTGTTAGTTGTAAAAATCATAGTAGAACGGCTGGTTTAGTTAATGGTCTTGGTGGAGGTACTGAAACCAAAGGCAATGTTTTGAATGGACGACAAGAGTTAACGCTTCAAAGGAATCGGAGTGTCAGGTATTCGACGAGTAATGTTGACACCGGGTTGTTGAGATTCTATTTGACACCGTTGAAGAGCTATAGGCGAAGCAGATCGGGAAAGAGTAGCTCAAAGGATTTGAATCCATCAGCCAGAAGTTTCATGTAA |
| Protein sequence |
>MsG0380014701.01.T01 MTSKTRRLTTCHRHPSQPITGFCASCLTERLSGIESSSADAEPELRRTKSCSGSRAGDQASSSAVEPRRRSCEILAPPEQNRGTLSDLFNIDDEKKKQKKKPGLNRCLENESRKSGFEIIDFDGGGDIVRVLGEDENDEEETKTMKEFIDLEIRSGKNAGRDSSRTSFWDAASVFSKRLRKWKRKQKLKRNNVDCDGGGVTGNGGGLRSMEVEKQRRRNLRETLSEVGEYGLSLGRRSCDTDPRLSVDDSRFSFDAPRASWDGYLIGKTCSRFSPMVAINDDRVLVEEEEDEEGEEEGVVNSESSVGGLYPGDSAETKHYYSDRRRRSFDRSNPRRKSAVIGDVDELRVISNAKVSPATTELFYGAKVLITEEDLRDANLKPKNSVNVQSDCRTGSASKEACDVEIGNDQKGTNKFHKWGKLWNKLGLVQKRKEDKLGEEECVAGDVVNKPIVESWQKLRRVVNGQGSESVSQKLIRSYSVSCKNHSRTAGLVNGLGGGTETKGNVLNGRQELTLQRNRSVRYSTSNVDTGLLRFYLTPLKSYRRSRSGKSSSKDLNPSARSFM* |
