AlfalfaGEDB | MsG0580024516.01

M. sativa cultivar ZhongmuNo.1 / MsG0580024516.01

KEGG: -
GO: GO:0005515
PFAM: PF01535 PF12854 PF13041

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580024516.01.T01	XP_003611313.1	95.568	519	23	0	1	519	1	519	0	1031

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580024516.01.T01	Q9FGL1	49.13	517	257	4	4	516	54	568	0	529

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580024516.01.T01	G7JW38	95.568	519	23	0	1	519	1	519	0.0	1031

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
MsG0280006933.01	MsG0580024516.01	PPI
MsG0280009685.01	MsG0580024516.01	PPI
MsG0580024516.01	MsG0780041163.01	PPI
MsG0580024516.01	MsG0780041163.01	PPI
MsG0580024516.01	MsG0880045133.01	PPI
MsG0780035948.01	MsG0580024516.01	PPI
MsG0780036302.01	MsG0580024516.01	PPI
MsG0780040456.01	MsG0580024516.01	PPI
MsG0780040456.01	MsG0580024516.01	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580024516.01.T01	MTR_5g012660	95.568	519	23	0	1	519	1	519	0.0	1031
MsG0580024516.01.T01	MTR_7g011100	34.783	506	316	5	21	516	15	516	5.22e-107	329
MsG0580024516.01.T01	MTR_7g011100	23.529	187	141	2	4	188	270	456	7.85e-16	80.5
MsG0580024516.01.T01	MTR_3g011950	33.458	535	315	6	21	516	151	683	1.03e-103	329
MsG0580024516.01.T01	MTR_3g011950	26.382	398	252	5	25	383	54	449	1.51e-39	153
MsG0580024516.01.T01	MTR_3g011950	24.033	362	233	6	4	324	235	595	1.16e-28	120
MsG0580024516.01.T01	MTR_1g014340	32.778	540	314	6	23	519	124	657	7.60e-98	311
MsG0580024516.01.T01	MTR_1g014340	28.605	423	257	7	4	384	2	421	1.19e-50	184
MsG0580024516.01.T01	MTR_1g014340	26.027	219	157	5	239	456	74	288	5.97e-20	94.0
MsG0580024516.01.T01	MTR_7g417770	32.110	545	328	5	12	517	278	819	1.45e-97	315

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580024516.01.T01	AT5G47460	49.130	517	257	4	4	516	54	568	0.0	529
MsG0580024516.01.T01	AT5G09950	32.342	538	320	5	21	515	323	859	1.00e-98	320
MsG0580024516.01.T01	AT5G09950	25.751	466	291	11	40	455	29	489	4.57e-41	158
MsG0580024516.01.T01	AT5G09950	24.557	395	252	9	5	354	409	802	2.38e-29	123
MsG0580024516.01.T01	AT5G09950	32.342	538	320	5	21	515	329	865	1.42e-98	320
MsG0580024516.01.T01	AT5G09950	25.751	466	291	11	40	455	35	495	5.64e-41	158
MsG0580024516.01.T01	AT5G09950	24.557	395	252	9	5	354	415	808	2.74e-29	123
MsG0580024516.01.T01	AT5G09950	32.342	538	320	5	21	515	329	865	1.42e-98	320
MsG0580024516.01.T01	AT5G09950	25.751	466	291	11	40	455	35	495	5.64e-41	158
MsG0580024516.01.T01	AT5G09950	24.557	395	252	9	5	354	415	808	2.74e-29	123

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Find 104 sgRNAs with CRISPR-Local

Find 116 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGGGTTAGGAAAGAAGC+AGG	0.529355	5:+5928307	MsG0580024516.01.T01:CDS
AAAGTATGGTTTGGATTCAT+CGG	0.494300	5:+5927688	MsG0580024516.01.T01:CDS
AACAATGGAAACAATGGACT+TGG	0.532295	5:+5927350	MsG0580024516.01.T01:CDS
AACTGCTTGATTGATATGTA+TGG	0.479489	5:+5927233	MsG0580024516.01.T01:CDS
AAGCACGTTGAATATTGATT+CGG	0.495181	5:-5927768	None:intergenic
AAGTTTCACCAGGCTAAGTT+GGG	0.474985	5:-5927150	None:intergenic
AATCAAGCAGTTAGCAACAA+CGG	0.670020	5:-5927222	None:intergenic
AATTTCGACAAACAAGTTGT+GGG	0.531894	5:-5926976	None:intergenic
AGCAGAGATTGCAGCTGCTA+AGG	0.458519	5:+5928174	MsG0580024516.01.T01:CDS
AGCATTGTTGTTCAATGATA+CGG	0.430736	5:+5928026	MsG0580024516.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AACTGCTTGATTGATATGTA+TGG	+	Chr5:5927233-5927252	MsG0580024516.01.T01:CDS	30.0%
AATTTCGACAAACAAGTTGT+GGG	-	Chr5:5926979-5926998	None:intergenic	30.0%
ATTGACATGTACTCAAAATG+TGG	+	Chr5:5927731-5927750	MsG0580024516.01.T01:CDS	30.0%
ATTGATATGTATGGCAAATG+CGG	+	Chr5:5927242-5927261	MsG0580024516.01.T01:CDS	30.0%
CAAAAGATGATACATGAACT+CGG	+	Chr5:5928082-5928101	MsG0580024516.01.T01:CDS	30.0%
CATTGAACAACAATGCTTAA+TGG	-	Chr5:5928023-5928042	None:intergenic	30.0%
CCATTATAAGAAACAACATC+AGG	-	Chr5:5927404-5927423	None:intergenic	30.0%
GGGAAGATGTTAATGTAATT+AGG	+	Chr5:5928269-5928288	MsG0580024516.01.T01:CDS	30.0%
TATTCAACGTGCTTTCTAAT+AGG	+	Chr5:5927777-5927796	MsG0580024516.01.T01:CDS	30.0%
TTAAGATATGTGATACCATC+AGG	-	Chr5:5927908-5927927	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr5	gene	5926798	5928357	5926798	ID=MsG0580024516.01;Name=MsG0580024516.01
Chr5	mRNA	5926798	5928357	5926798	ID=MsG0580024516.01.T01;Parent=MsG0580024516.01;Name=MsG0580024516.01.T01;_AED=0.01;_eAED=0.01;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|519
Chr5	exon	5926798	5928357	5926798	ID=MsG0580024516.01.T01:exon:19130;Parent=MsG0580024516.01.T01
Chr5	CDS	5926798	5928357	5926798	ID=MsG0580024516.01.T01:cds;Parent=MsG0580024516.01.T01

Gene Sequence

>MsG0580024516.01.T01

ATGACTAAAAATGCATTGGCTCTTGTTGACCTCATTCGAACTGCAACTGACCTTTGCTGCCTCAAATTCGGACAACAAATCCACAGTTATGCAATACGTTCTGGTTATCATCATTCAAATATCTATGTCTCCACTTCCCTCGTCAAATTCTATGTCAAAATGCACTTTTTCACTCATGCCCACAACTTGTTTGTCGAAATTCCTCAACCAAATGTCGTTTCTTGGAACACTTTGATTTCTGGTTATGTCCATGCTGGCCGGTTTAAAAAAGCATTGTCTCTTTTCACAAACCTAGAAAGATCTCAAATTCGTGCTGACGCGTTCTCTTTCACGTCTGCTATGGTTGCTTGTGCCCAACTTAGCCTGGTGAAACTTGGTAGTTCAATTCATTCCAAGACAGTGAAATTAGGAATGGATAATAACACCGTTGTTGCTAACTGCTTGATTGATATGTATGGCAAATGCGGATCTGTTGAACGTGCTGTTCGGATATTCTCTGATATCGTTGACAAGGATGTTATTTCTTGGAATTCAGTTATAGCCGCATGTGCAAACAATGGAAACAATGGACTTGGTTTCAAGTTTTTGCAACTCATGCCTAACCCTGATGTTGTTTCTTATAATGGGATGATAAATGGAATTGCTCAAGCTGGGAAGATAGAAGACGCTGTTCGGATTTTGTCAACTATGCCGTGTCCAAATTCATCTTCTTGGAACTCTGTAATTACTGGATTTGTGAATAGGAGTCGAGTTCCTGAAGCTTTGGAAATGTTCAGTAAAATGCATTTGAGAAACTTGCAGATGGATGAGTTCACATTTTCAATAATTCTAAACGGAATTGCTAGTCTGTCAGCATTAACATGGGGGATGCTGATTCATTGTTGTACAATAAAGTATGGTTTGGATTCATCGGTTGTTGTAGGGAGTTCCCTGATTGACATGTACTCAAAATGTGGGCAGGTGAACGATGCCGAATCAATATTCAACGTGCTTTCTAATAGGAATTTGGTAAGCTGGAATGCAATGATATATGGGTATGCTCGCAACGGTGATTCTGCACAAGCTATTAGCCTATTCGAGTTGTTGAAAATGGAAAGAGACACAAAACCTGATGGTATCACATATCTTAATGTTATATCGGCATGTTCACATAGCCAAATACCATTTGAGATGGGTATTCAGTATTTTGATGATATGATTAATGAATATGGGATTGCACCGTCCATTAAGCATTGTTGTTCAATGATACGGCTAATGGGGCAAAAAGGAGAGTTGTCGAGAGCACAAAAGATGATACATGAACTCGGTTTTGAGTCATGTGGAGTGGTTTGGAGGTCTTTGCTTGCTGCTTGTGGAACTCAGGAAGATTTACAAGTAGCAGAGATTGCAGCTGCTAAGGTGATTGAACTGGAGAAGGATGAAGATTACGTCTACGTGATGTTGTCTAATATGTATGCATCTTTTGGAAGATGGGAAGATGTTAATGTAATTAGGAGCCTCATGAGTAAGAAAAGGGTTAGGAAAGAAGCAGGTTCTAGCTGGATAGAAGTAAATAGATAG

Protein sequence

>MsG0580024516.01.T01

MTKNALALVDLIRTATDLCCLKFGQQIHSYAIRSGYHHSNIYVSTSLVKFYVKMHFFTHAHNLFVEIPQPNVVSWNTLISGYVHAGRFKKALSLFTNLERSQIRADAFSFTSAMVACAQLSLVKLGSSIHSKTVKLGMDNNTVVANCLIDMYGKCGSVERAVRIFSDIVDKDVISWNSVIAACANNGNNGLGFKFLQLMPNPDVVSYNGMINGIAQAGKIEDAVRILSTMPCPNSSSWNSVITGFVNRSRVPEALEMFSKMHLRNLQMDEFTFSIILNGIASLSALTWGMLIHCCTIKYGLDSSVVVGSSLIDMYSKCGQVNDAESIFNVLSNRNLVSWNAMIYGYARNGDSAQAISLFELLKMERDTKPDGITYLNVISACSHSQIPFEMGIQYFDDMINEYGIAPSIKHCCSMIRLMGQKGELSRAQKMIHELGFESCGVVWRSLLAACGTQEDLQVAEIAAAKVIELEKDEDYVYVMLSNMYASFGRWEDVNVIRSLMSKKRVRKEAGSSWIEVNR*