Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024743.01.T01 | XP_003611595.1 | 67.466 | 292 | 19 | 2 | 1 | 216 | 62 | 353 | 1.41E-119 | 353 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024743.01.T01 | Q2V3B2 | 51.579 | 95 | 41 | 2 | 56 | 145 | 117 | 211 | 1.94E-21 | 93.2 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024743.01.T01 | G7K3T3 | 67.466 | 292 | 19 | 2 | 1 | 216 | 62 | 353 | 6.74e-120 | 353 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0480023620.01 | MsG0580024743.01 | 0.830803 | 2.390700e-55 | 2.546042e-52 |
| MsG0580024743.01 | MsG0580024744.01 | 0.816574 | 5.154219e-52 | 3.665872e-49 |
| MsG0580024743.01 | MsG0780039654.01 | 0.802524 | 5.384789e-49 | 2.643451e-46 |
| MsG0580024743.01 | MsG0780039655.01 | 0.806665 | 7.367951e-50 | 4.022153e-47 |
| MsG0580024743.01 | MsG0780039656.01 | 0.804964 | 1.677962e-49 | 8.770116e-47 |
| MsG0580024743.01 | MsG0880047226.01 | 0.818670 | 1.736948e-52 | 1.308479e-49 |
| MsG0280010001.01 | MsG0580024743.01 | 0.805760 | 1.142530e-49 | 6.095258e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024743.01.T01 | MTR_5g015670 | 67.466 | 292 | 19 | 2 | 1 | 216 | 62 | 353 | 1.71e-123 | 353 |
| MsG0580024743.01.T01 | MTR_3g103080 | 37.500 | 176 | 63 | 4 | 1 | 145 | 42 | 201 | 2.72e-27 | 106 |
| MsG0580024743.01.T01 | MTR_3g100710 | 47.727 | 88 | 43 | 1 | 41 | 125 | 96 | 183 | 1.51e-20 | 88.2 |
| MsG0580024743.01.T01 | MTR_4g080990 | 47.436 | 78 | 38 | 1 | 50 | 124 | 129 | 206 | 8.44e-18 | 80.9 |
| MsG0580024743.01.T01 | MTR_3g102310 | 34.254 | 181 | 80 | 6 | 50 | 203 | 122 | 290 | 1.94e-17 | 79.7 |
| MsG0580024743.01.T01 | MTR_4g094942 | 46.429 | 84 | 42 | 1 | 50 | 130 | 119 | 202 | 4.05e-17 | 79.0 |
| MsG0580024743.01.T01 | MTR_5g035360 | 43.878 | 98 | 43 | 2 | 52 | 137 | 116 | 213 | 5.48e-17 | 78.6 |
| MsG0580024743.01.T01 | MTR_3g100710 | 47.436 | 78 | 38 | 1 | 50 | 124 | 134 | 211 | 1.05e-16 | 77.8 |
| MsG0580024743.01.T01 | MTR_8g063120 | 42.268 | 97 | 52 | 2 | 52 | 144 | 133 | 229 | 1.85e-16 | 76.3 |
| MsG0580024743.01.T01 | MTR_8g063120 | 42.268 | 97 | 52 | 2 | 52 | 144 | 133 | 229 | 3.14e-16 | 76.6 |
| MsG0580024743.01.T01 | MTR_1g107535 | 46.835 | 79 | 39 | 1 | 50 | 125 | 144 | 222 | 8.26e-16 | 75.5 |
| MsG0580024743.01.T01 | MTR_5g032550 | 44.872 | 78 | 40 | 1 | 50 | 124 | 129 | 206 | 5.49e-15 | 72.0 |
| MsG0580024743.01.T01 | MTR_5g032550 | 44.872 | 78 | 40 | 1 | 50 | 124 | 129 | 206 | 9.50e-15 | 72.4 |
| MsG0580024743.01.T01 | MTR_5g065830 | 39.362 | 94 | 51 | 3 | 43 | 133 | 120 | 210 | 1.58e-13 | 68.9 |
| MsG0580024743.01.T01 | MTR_4g095790 | 26.459 | 257 | 100 | 7 | 30 | 201 | 56 | 308 | 1.66e-13 | 68.6 |
| MsG0580024743.01.T01 | MTR_2g011500 | 35.577 | 104 | 60 | 2 | 50 | 147 | 121 | 223 | 2.57e-11 | 62.0 |
| MsG0580024743.01.T01 | MTR_8g068950 | 41.772 | 79 | 42 | 2 | 50 | 124 | 145 | 223 | 9.96e-11 | 60.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024743.01.T01 | AT4G37630 | 48.515 | 101 | 47 | 2 | 50 | 145 | 111 | 211 | 4.00e-23 | 94.0 |
| MsG0580024743.01.T01 | AT4G37630 | 49.000 | 100 | 46 | 2 | 51 | 145 | 110 | 209 | 4.61e-23 | 94.0 |
| MsG0580024743.01.T01 | AT4G37630 | 51.579 | 95 | 41 | 2 | 56 | 145 | 117 | 211 | 1.98e-22 | 93.2 |
| MsG0580024743.01.T01 | AT4G34160 | 45.000 | 100 | 51 | 2 | 50 | 145 | 124 | 223 | 1.92e-19 | 85.9 |
| MsG0580024743.01.T01 | AT2G22490 | 46.591 | 88 | 43 | 2 | 54 | 138 | 138 | 224 | 1.14e-17 | 80.5 |
| MsG0580024743.01.T01 | AT2G22490 | 46.591 | 88 | 43 | 2 | 54 | 138 | 138 | 224 | 1.17e-17 | 80.5 |
| MsG0580024743.01.T01 | AT1G70210 | 44.000 | 100 | 52 | 2 | 52 | 148 | 121 | 219 | 5.80e-17 | 78.6 |
| MsG0580024743.01.T01 | AT5G10440 | 43.158 | 95 | 45 | 3 | 53 | 144 | 109 | 197 | 8.92e-17 | 76.3 |
| MsG0580024743.01.T01 | AT5G10440 | 43.158 | 95 | 45 | 3 | 53 | 144 | 109 | 197 | 1.59e-16 | 76.6 |
| MsG0580024743.01.T01 | AT5G65420 | 39.175 | 97 | 54 | 2 | 53 | 144 | 118 | 214 | 2.52e-16 | 75.5 |
| MsG0580024743.01.T01 | AT5G65420 | 39.175 | 97 | 54 | 2 | 53 | 144 | 128 | 224 | 3.63e-16 | 75.9 |
| MsG0580024743.01.T01 | AT5G65420 | 39.175 | 97 | 54 | 2 | 53 | 144 | 118 | 214 | 3.72e-16 | 75.5 |
| MsG0580024743.01.T01 | AT5G65420 | 39.583 | 96 | 53 | 2 | 54 | 144 | 119 | 214 | 3.78e-16 | 75.9 |
| MsG0580024743.01.T01 | AT3G50070 | 31.544 | 149 | 79 | 4 | 1 | 145 | 93 | 222 | 8.63e-16 | 75.5 |
| MsG0580024743.01.T01 | AT5G67260 | 48.750 | 80 | 36 | 2 | 54 | 130 | 137 | 214 | 1.06e-14 | 72.4 |
| MsG0580024743.01.T01 | AT5G67260 | 48.750 | 80 | 36 | 2 | 54 | 130 | 137 | 214 | 1.16e-14 | 72.4 |
Find 28 sgRNAs with CRISPR-Local
Find 54 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAGTACTTTGGAATGGAAAA+TGG | 0.144972 | 5:-9074066 | MsG0580024743.01.T01:CDS |
| TCTTGATCCATTGCAGAATT+TGG | 0.222300 | 5:+9074004 | None:intergenic |
| TTCTCAAATCGATATTCTAT+TGG | 0.242040 | 5:+9074122 | None:intergenic |
| ATTCAAGCAAAAGCTTATTC+TGG | 0.285826 | 5:-9074568 | MsG0580024743.01.T01:CDS |
| CAAGAAACAGGACTTGGATT+TGG | 0.320103 | 5:-9074647 | MsG0580024743.01.T01:CDS |
| AGTTAACAAGACACCTTTAT+CGG | 0.362687 | 5:-9073559 | MsG0580024743.01.T01:CDS |
| GTTGATAATAAATCCGATAA+AGG | 0.374551 | 5:+9073546 | None:intergenic |
| AGTACTTTGGAATGGAAAAT+GGG | 0.395838 | 5:-9074065 | MsG0580024743.01.T01:CDS |
| CTCGTTTGCATGCTATTGAT+TGG | 0.395951 | 5:-9074535 | MsG0580024743.01.T01:CDS |
| TCTATTGGATATTCTGATAG+TGG | 0.404279 | 5:+9074137 | None:intergenic |
| TAAGAGAAAGCTTGGTTATG+AGG | 0.430380 | 5:-9073466 | MsG0580024743.01.T01:CDS |
| AAATAATGCAAATAAGCAAA+AGG | 0.447047 | 5:+9074032 | None:intergenic |
| AATAATGCAAATAAGCAAAA+GGG | 0.449345 | 5:+9074033 | None:intergenic |
| TATGCAGGAATCAAAACCTT+GGG | 0.450693 | 5:-9074225 | MsG0580024743.01.T01:intron |
| TATTTGTTCAAACAAGAAAC+AGG | 0.453564 | 5:-9074659 | MsG0580024743.01.T01:CDS |
| GGCTATTCAATTGTTGTCAG+TGG | 0.484737 | 5:-9074204 | MsG0580024743.01.T01:CDS |
| AACAAATCTAATGCTTGTGC+AGG | 0.488051 | 5:-9073492 | MsG0580024743.01.T01:CDS |
| GCAGGAGTTAAGAGAAAGCT+TGG | 0.497942 | 5:-9073474 | MsG0580024743.01.T01:CDS |
| TTCAAACAAGAAACAGGACT+TGG | 0.508140 | 5:-9074653 | MsG0580024743.01.T01:CDS |
| TTCTTCACCAAATTCTGCAA+TGG | 0.509880 | 5:-9074011 | MsG0580024743.01.T01:CDS |
| TTATGCAGGAATCAAAACCT+TGG | 0.516551 | 5:-9074226 | MsG0580024743.01.T01:intron |
| TGCAAATAAGCAAAAGGGGT+TGG | 0.517338 | 5:+9074038 | None:intergenic |
| CCACACAACACTTGTGACAT+TGG | 0.521424 | 5:-9073958 | MsG0580024743.01.T01:intron |
| GAATAAAGTTATTAAGAACA+TGG | 0.585219 | 5:-9074102 | MsG0580024743.01.T01:CDS |
| ATAATGCAAATAAGCAAAAG+GGG | 0.614789 | 5:+9074034 | None:intergenic |
| GACAACAATTGAATAGCCCA+AGG | 0.617594 | 5:+9074209 | None:intergenic |
| ATTGGATATTCTGATAGTGG+AGG | 0.627410 | 5:+9074140 | None:intergenic |
| CCAATGTCACAAGTGTTGTG+TGG | 0.680777 | 5:+9073958 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTATCAATTAATTATTTGAT+CGG | - | Chr5:9073718-9073737 | MsG0580024743.01.T01:intron | 10.0% |
| !!! | AAAACAAATTTTGATTAGTT+TGG | + | Chr5:9073584-9073603 | None:intergenic | 15.0% |
| !!! | CTAATTTATTTATTTTATGC+AGG | - | Chr5:9073832-9073851 | MsG0580024743.01.T01:intron | 15.0% |
| !! | AAATAATGCAAATAAGCAAA+AGG | + | Chr5:9074043-9074062 | None:intergenic | 20.0% |
| !! | AATAATGCAAATAAGCAAAA+GGG | + | Chr5:9074042-9074061 | None:intergenic | 20.0% |
| !! | GAATAAAGTTATTAAGAACA+TGG | - | Chr5:9073970-9073989 | MsG0580024743.01.T01:CDS | 20.0% |
| ! | AAAACAATGAAAGAGGAAAA+AGG | + | Chr5:9073617-9073636 | None:intergenic | 25.0% |
| ! | ATAATGCAAATAAGCAAAAG+GGG | + | Chr5:9074041-9074060 | None:intergenic | 25.0% |
| ! | ATAGCATAAAAAATGAGTGA+TGG | + | Chr5:9073453-9073472 | None:intergenic | 25.0% |
| ! | TTCTCAAATCGATATTCTAT+TGG | + | Chr5:9073953-9073972 | None:intergenic | 25.0% |
| ! | TTTATCAATTGCAGCAAAAA+TGG | - | Chr5:9073895-9073914 | MsG0580024743.01.T01:intron | 25.0% |
| !! | ATTAGTTTAATCTCATCATG+TGG | - | Chr5:9074334-9074353 | MsG0580024743.01.T01:intron | 25.0% |
| !!! | ACTTTTGATTTTGAGTACTT+TGG | - | Chr5:9073994-9074013 | MsG0580024743.01.T01:CDS | 25.0% |
| !!! | GTTGATAATAAATCCGATAA+AGG | + | Chr5:9074529-9074548 | None:intergenic | 25.0% |
| !!! | TTGTTTTTGTTTTATGCAAC+AGG | - | Chr5:9074441-9074460 | MsG0580024743.01.T01:intron | 25.0% |
| !!! | TTTCCTCTTTCATTGTTTTT+TGG | - | Chr5:9073618-9073637 | MsG0580024743.01.T01:intron | 25.0% |
| AGTTAACAAGACACCTTTAT+CGG | - | Chr5:9074513-9074532 | MsG0580024743.01.T01:intron | 30.0% | |
| ATGTGTTAAGCTTACATCAA+TGG | + | Chr5:9073760-9073779 | None:intergenic | 30.0% | |
| ATTCAAGCAAAAGCTTATTC+TGG | - | Chr5:9073504-9073523 | MsG0580024743.01.T01:CDS | 30.0% | |
| CAACCAAAAAACAATGAAAG+AGG | + | Chr5:9073624-9073643 | None:intergenic | 30.0% | |
| TCTATTGGATATTCTGATAG+TGG | + | Chr5:9073938-9073957 | None:intergenic | 30.0% | |
| ! | TTGATCGGTTTCTTTCAAAA+CGG | - | Chr5:9073733-9073752 | MsG0580024743.01.T01:intron | 30.0% |
| !! | AGTACTTTGGAATGGAAAAT+GGG | - | Chr5:9074007-9074026 | MsG0580024743.01.T01:CDS | 30.0% |
| !! | TGATTTTGAGTACTTTGGAA+TGG | - | Chr5:9073999-9074018 | MsG0580024743.01.T01:CDS | 30.0% |
| !! | TTATTTGTTGATTCAGACAC+AGG | - | Chr5:9073663-9073682 | MsG0580024743.01.T01:intron | 30.0% |
| !!! | AATTTCCTTTTGGGTTAATG+TGG | + | Chr5:9074305-9074324 | None:intergenic | 30.0% |
| AACAAATCTAATGCTTGTGC+AGG | - | Chr5:9074580-9074599 | MsG0580024743.01.T01:CDS | 35.0% | |
| ATTCAGACACAGGCAAAATT+TGG | - | Chr5:9073673-9073692 | MsG0580024743.01.T01:intron | 35.0% | |
| CCCAAAAGGAAATTGATCTT+CGG | - | Chr5:9074311-9074330 | MsG0580024743.01.T01:intron | 35.0% | |
| GAAGCAGAAGCAATTATAGA+TGG | + | Chr5:9074262-9074281 | None:intergenic | 35.0% | |
| TAAGAGAAAGCTTGGTTATG+AGG | - | Chr5:9074606-9074625 | MsG0580024743.01.T01:CDS | 35.0% | |
| TATGCAGGAATCAAAACCTT+GGG | - | Chr5:9073847-9073866 | MsG0580024743.01.T01:intron | 35.0% | |
| TCTTGATCCATTGCAGAATT+TGG | + | Chr5:9074071-9074090 | None:intergenic | 35.0% | |
| TGATTCCACATTAACCCAAA+AGG | - | Chr5:9074297-9074316 | MsG0580024743.01.T01:intron | 35.0% | |
| TTATGCAGGAATCAAAACCT+TGG | - | Chr5:9073846-9073865 | MsG0580024743.01.T01:intron | 35.0% | |
| TTCAAACAAGAAACAGGACT+TGG | - | Chr5:9073419-9073438 | MsG0580024743.01.T01:CDS | 35.0% | |
| TTCTTCACCAAATTCTGCAA+TGG | - | Chr5:9074061-9074080 | MsG0580024743.01.T01:CDS | 35.0% | |
| ! | AACATTGAAGATTTTCCTCC+AGG | - | Chr5:9074634-9074653 | MsG0580024743.01.T01:CDS | 35.0% |
| ! | CCGAAGATCAATTTCCTTTT+GGG | + | Chr5:9074314-9074333 | None:intergenic | 35.0% |
| ! | TCCGAAGATCAATTTCCTTT+TGG | + | Chr5:9074315-9074334 | None:intergenic | 35.0% |
| !! | ATTGGATATTCTGATAGTGG+AGG | + | Chr5:9073935-9073954 | None:intergenic | 35.0% |
| !! | GAGTACTTTGGAATGGAAAA+TGG | - | Chr5:9074006-9074025 | MsG0580024743.01.T01:CDS | 35.0% |
| ACACTTGTGACATTGGTCAA+AGG | - | Chr5:9074121-9074140 | MsG0580024743.01.T01:CDS | 40.0% | |
| CAAGAAACAGGACTTGGATT+TGG | - | Chr5:9073425-9073444 | MsG0580024743.01.T01:CDS | 40.0% | |
| CTCGTTTGCATGCTATTGAT+TGG | - | Chr5:9073537-9073556 | MsG0580024743.01.T01:CDS | 40.0% | |
| GACAACAATTGAATAGCCCA+AGG | + | Chr5:9073866-9073885 | None:intergenic | 40.0% | |
| GGCTATTCAATTGTTGTCAG+TGG | - | Chr5:9073868-9073887 | MsG0580024743.01.T01:intron | 40.0% | |
| TGCAAATAAGCAAAAGGGGT+TGG | + | Chr5:9074037-9074056 | None:intergenic | 40.0% | |
| ! | GTTTTCAACAACAACACTCG+TGG | + | Chr5:9074563-9074582 | None:intergenic | 40.0% |
| CCACACAACACTTGTGACAT+TGG | - | Chr5:9074114-9074133 | MsG0580024743.01.T01:CDS | 45.0% | |
| GCAGGAGTTAAGAGAAAGCT+TGG | - | Chr5:9074598-9074617 | MsG0580024743.01.T01:CDS | 45.0% | |
| ! | CCAATGTCACAAGTGTTGTG+TGG | + | Chr5:9074117-9074136 | None:intergenic | 45.0% |
| ! | GGTTGACGTAGCTTTTGTCC+TGG | + | Chr5:9074655-9074674 | None:intergenic | 50.0% |
| ! | TGACGTAGCTTTTGTCCTGG+AGG | + | Chr5:9074652-9074671 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 9073416 | 9074678 | 9073416 | ID=MsG0580024743.01;Name=MsG0580024743.01 |
| Chr5 | mRNA | 9073416 | 9074678 | 9073416 | ID=MsG0580024743.01.T01;Parent=MsG0580024743.01;Name=MsG0580024743.01.T01;_AED=0.26;_eAED=0.26;_QI=0|0|0|1|1|1|3|0|216 |
| Chr5 | exon | 9074526 | 9074678 | 9074526 | ID=MsG0580024743.01.T01:exon:1873;Parent=MsG0580024743.01.T01 |
| Chr5 | exon | 9073959 | 9074240 | 9073959 | ID=MsG0580024743.01.T01:exon:1872;Parent=MsG0580024743.01.T01 |
| Chr5 | exon | 9073416 | 9073631 | 9073416 | ID=MsG0580024743.01.T01:exon:1871;Parent=MsG0580024743.01.T01 |
| Chr5 | CDS | 9074526 | 9074678 | 9074526 | ID=MsG0580024743.01.T01:cds;Parent=MsG0580024743.01.T01 |
| Chr5 | CDS | 9073959 | 9074240 | 9073959 | ID=MsG0580024743.01.T01:cds;Parent=MsG0580024743.01.T01 |
| Chr5 | CDS | 9073416 | 9073631 | 9073416 | ID=MsG0580024743.01.T01:cds;Parent=MsG0580024743.01.T01 |
| Gene Sequence |
| Protein sequence |
