AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280010001.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280010001.01.T01 MTR_2g075910 97.895 475 10 0 1 475 1 475 0.0 962
MsG0280010001.01.T01 MTR_8g059485 59.870 461 156 10 1 455 1 438 3.41e-180 512
MsG0280010001.01.T01 MTR_8g059485 59.870 461 156 10 1 455 10 447 1.02e-179 511
MsG0280010001.01.T01 MTR_8g087400 45.356 463 238 7 1 453 1 458 3.91e-127 377
MsG0280010001.01.T01 MTR_4g025420 41.282 390 211 5 71 453 34 412 1.21e-98 303
MsG0280010001.01.T01 MTR_0178s0030 31.486 397 247 5 69 453 139 522 1.18e-57 199
MsG0280010001.01.T01 MTR_5g075180 31.738 397 233 8 64 455 78 441 2.67e-51 181
MsG0280010001.01.T01 MTR_3g462150 30.446 404 248 10 73 457 92 481 9.46e-50 177
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280010001.01.T01 AT1G53380 52.288 459 204 7 1 453 1 450 1.77e-167 480
MsG0280010001.01.T01 AT1G53380 52.288 459 204 7 1 453 1 450 1.77e-167 480
MsG0280010001.01.T01 AT1G53380 52.288 459 204 7 1 453 1 450 1.77e-167 480
MsG0280010001.01.T01 AT1G53380 52.288 459 204 7 1 453 1 450 1.77e-167 480
MsG0280010001.01.T01 AT1G53380 52.288 459 204 7 1 453 1 450 1.77e-167 480
MsG0280010001.01.T01 AT3G14870 50.318 471 216 5 1 458 5 470 8.35e-165 474
MsG0280010001.01.T01 AT3G14870 50.318 471 216 5 1 458 2 467 9.07e-165 474
MsG0280010001.01.T01 AT3G14870 50.318 471 216 5 1 458 1 466 1.28e-164 473
MsG0280010001.01.T01 AT3G14870 50.318 471 216 5 1 458 1 466 1.28e-164 473
MsG0280010001.01.T01 AT3G14870 50.318 471 216 5 1 458 1 466 1.28e-164 473
MsG0280010001.01.T01 AT1G29300 41.841 478 234 9 1 457 1 455 5.33e-111 336
MsG0280010001.01.T01 AT2G45260 40.351 399 225 3 62 453 26 418 4.07e-102 312
MsG0280010001.01.T01 AT3G60680 31.714 391 251 4 74 459 120 499 1.17e-59 204
MsG0280010001.01.T01 AT4G34080 36.786 280 170 2 62 334 2 281 2.71e-56 189
MsG0280010001.01.T01 AT2G30380 32.540 378 212 4 76 453 76 410 6.67e-54 186
MsG0280010001.01.T01 AT4G33320 34.965 286 178 3 62 339 2 287 4.59e-50 172
MsG0280010001.01.T01 AT5G58960 28.606 416 247 9 73 456 86 483 1.96e-46 168
MsG0280010001.01.T01 AT5G58960 28.606 416 247 9 73 456 86 483 1.96e-46 168
MsG0280010001.01.T01 AT5G58960 28.606 416 247 9 73 456 161 558 5.38e-46 168
MsG0280010001.01.T01 AT2G32130 45.161 124 68 0 73 196 34 157 6.95e-27 106
MsG0280010001.01.T01 AT4G36100 45.098 102 53 1 62 160 2 103 2.05e-20 90.5

Find 52 sgRNAs with CRISPR-Local

Find 72 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATAATGTTGTTCACTTATC+AGG 0.152485 2:+62653807 MsG0280010001.01.T01:CDS
TCATTTGGAAGATTGAAATT+AGG 0.213794 2:-62654047 None:intergenic
GCTAGCATTTCAACCGTTAA+AGG 0.254354 2:+62653441 MsG0280010001.01.T01:CDS
TCCATGTTTGTTTCATCCTT+TGG 0.256313 2:-62653315 None:intergenic
CTTCTTCGCGGTAATCTCAT+AGG 0.337313 2:-62653658 None:intergenic
CATTTGGAAGATTGAAATTA+GGG 0.340570 2:-62654046 None:intergenic
ATGAAATGAGATCTGCAAAA+TGG 0.347278 2:+62653907 MsG0280010001.01.T01:CDS
TTCAGCATATGCACCTTTAA+CGG 0.355170 2:-62653454 None:intergenic
AGCTGAATCGAAGGAGATAA+AGG 0.357870 2:+62653623 MsG0280010001.01.T01:CDS
TTCCTAGCTAATTTCTTGCT+TGG 0.381111 2:-62653231 None:intergenic
TCATCAGGAAGTGCCTCATT+TGG 0.398030 2:-62654062 None:intergenic
GAAATCTTTCAAGTGGGAAA+AGG 0.412349 2:+62654377 MsG0280010001.01.T01:CDS
GATTAACTCCTGTGTGAAAT+CGG 0.417828 2:-62654608 None:intergenic
TCAGAAGCTTCAATTCCATC+AGG 0.419938 2:-62653504 None:intergenic
TTGCTTGGTGTCACTGCTGA+AGG 0.427239 2:-62653216 None:intergenic
GTTTCTTAAAGAGAAATTAG+TGG 0.430754 2:+62653719 MsG0280010001.01.T01:CDS
TCAGAAGAAGAACCAGTTGT+TGG 0.431414 2:+62654476 MsG0280010001.01.T01:CDS
AATTGCGCAAACGATGATCT+TGG 0.434932 2:-62654167 None:intergenic
GAAACTCTCCACCACTCAAA+AGG 0.448344 2:-62654265 None:intergenic
CGCGAAGAAGTTGGAATCAC+AGG 0.450315 2:+62653671 MsG0280010001.01.T01:CDS
CATCGTTTGCGCAATTCTGC+AGG 0.469810 2:+62654174 MsG0280010001.01.T01:CDS
TGCTCTAAGATGAAGAACTT+TGG 0.476082 2:-62653259 None:intergenic
AGCTTGAACCTCAAAAGAAA+AGG 0.479323 2:-62654354 None:intergenic
AAATCTTTCAAGTGGGAAAA+GGG 0.495316 2:+62654378 MsG0280010001.01.T01:CDS
TCAATTCCATCAGGGTCAAA+AGG 0.497708 2:-62653495 None:intergenic
CTGTGATTCCAACTTCTTCG+CGG 0.499296 2:-62653670 None:intergenic
ATAATGTTGTTCACTTATCA+GGG 0.499714 2:+62653808 MsG0280010001.01.T01:CDS
TAATTTCAATCTTCCAAATG+AGG 0.502545 2:+62654049 MsG0280010001.01.T01:CDS
GCTGCAAGTATCGCTTTCTC+TGG 0.503251 2:-62653603 None:intergenic
TGAGATTACCGCGAAGAAGT+TGG 0.506310 2:+62653662 MsG0280010001.01.T01:CDS
ACAACAGTAAAACCAACAAC+TGG 0.508318 2:-62654488 None:intergenic
TGAAATGAGATCTGCAAAAT+GGG 0.517744 2:+62653908 MsG0280010001.01.T01:CDS
AGAAGTTGGAATCACAGGTT+AGG 0.521372 2:+62653676 MsG0280010001.01.T01:CDS
GCTGAATCGAAGGAGATAAA+GGG 0.532899 2:+62653624 MsG0280010001.01.T01:CDS
AAAGGATCACGATCGCGTGC+TGG 0.540329 2:+62654584 MsG0280010001.01.T01:CDS
GAGAAGAGATTGAATCAAAG+TGG 0.546796 2:+62653768 MsG0280010001.01.T01:CDS
TGGAAAAGACTACAATAACT+TGG 0.552801 2:+62653334 MsG0280010001.01.T01:CDS
TGAGTTGATTCTTCTGCATG+AGG 0.560321 2:-62654556 None:intergenic
AAGAATCAACTCAAGGTGAA+AGG 0.569431 2:+62654566 MsG0280010001.01.T01:CDS
TCAAGCTGAAATCTTTCAAG+TGG 0.572316 2:+62654370 MsG0280010001.01.T01:CDS
CAAGCTGAAATCTTTCAAGT+GGG 0.598623 2:+62654371 MsG0280010001.01.T01:CDS
TCCAAAGGATGAAACAAACA+TGG 0.604906 2:+62653314 MsG0280010001.01.T01:CDS
GATACTTGCAGCTGAATCGA+AGG 0.610878 2:+62653614 MsG0280010001.01.T01:CDS
CAGAAGCTTCAATTCCATCA+GGG 0.612641 2:-62653503 None:intergenic
TATATCTGATACAAATCCAA+AGG 0.615715 2:+62653299 MsG0280010001.01.T01:CDS
GATCGATTCGAAACTTTGTG+AGG 0.630009 2:+62653874 MsG0280010001.01.T01:CDS
AGTATCGCTTTCTCTGGTGA+AGG 0.630806 2:-62653597 None:intergenic
CACCAAGCAAGAAATTAGCT+AGG 0.637970 2:+62653229 MsG0280010001.01.T01:CDS
AAAACAGTAGCAAAATGGCT+AGG 0.639681 2:-62653837 None:intergenic
CATGCAGAAGAATCAACTCA+AGG 0.660821 2:+62654559 MsG0280010001.01.T01:CDS
GTGCTGGTCCGATTTCACAC+AGG 0.697518 2:+62654600 MsG0280010001.01.T01:CDS
TGATGCTATTGAACACAATG+TGG 0.709156 2:+62653947 MsG0280010001.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAGAAAAAATCAACAAAAT+TGG + Chr2:62654084-62654103 MsG0280010001.01.T01:CDS 15.0%
! ATAATGTTGTTCACTTATCA+GGG + Chr2:62653808-62653827 MsG0280010001.01.T01:CDS 25.0%
! CATTTGGAAGATTGAAATTA+GGG - Chr2:62654049-62654068 None:intergenic 25.0%
! GTTTCTTAAAGAGAAATTAG+TGG + Chr2:62653719-62653738 MsG0280010001.01.T01:CDS 25.0%
! TAATTTCAATCTTCCAAATG+AGG + Chr2:62654049-62654068 MsG0280010001.01.T01:CDS 25.0%
! TATATCTGATACAAATCCAA+AGG + Chr2:62653299-62653318 MsG0280010001.01.T01:CDS 25.0%
! TCATTTGGAAGATTGAAATT+AGG - Chr2:62654050-62654069 None:intergenic 25.0%
!! AAAAATGATGACTCCATTTT+TGG - Chr2:62654224-62654243 None:intergenic 25.0%
!!! AAAATGGAGTCATCATTTTT+TGG + Chr2:62654224-62654243 MsG0280010001.01.T01:CDS 25.0%
!!! GATGCAAAAAAATCACTTTT+TGG - Chr2:62654290-62654309 None:intergenic 25.0%
!!! TGTTGATTTTTTCTATCATC+AGG - Chr2:62654080-62654099 None:intergenic 25.0%
ATGAAATGAGATCTGCAAAA+TGG + Chr2:62653907-62653926 MsG0280010001.01.T01:CDS 30.0%
GATAATGTTGTTCACTTATC+AGG + Chr2:62653807-62653826 MsG0280010001.01.T01:CDS 30.0%
TGAAATGAGATCTGCAAAAT+GGG + Chr2:62653908-62653927 MsG0280010001.01.T01:CDS 30.0%
TGGAAAAGACTACAATAACT+TGG + Chr2:62653334-62653353 MsG0280010001.01.T01:CDS 30.0%
! AAATCTTTCAAGTGGGAAAA+GGG + Chr2:62654378-62654397 MsG0280010001.01.T01:CDS 30.0%
! TAGATTTTCTGATGTGTACA+TGG + Chr2:62654403-62654422 MsG0280010001.01.T01:CDS 30.0%
! TTTTGCATCTTTTGCTGAAA+TGG + Chr2:62654301-62654320 MsG0280010001.01.T01:CDS 30.0%
!! TTGTTTAGCCTTTTCTTTTG+AGG + Chr2:62654346-62654365 MsG0280010001.01.T01:CDS 30.0%
AAAACAGTAGCAAAATGGCT+AGG - Chr2:62653840-62653859 None:intergenic 35.0%
AAGAATCAACTCAAGGTGAA+AGG + Chr2:62654566-62654585 MsG0280010001.01.T01:CDS 35.0%
ACAACAGTAAAACCAACAAC+TGG - Chr2:62654491-62654510 None:intergenic 35.0%
AGCTTGAACCTCAAAAGAAA+AGG - Chr2:62654357-62654376 None:intergenic 35.0%
GACGAAAAACAGTAGCAAAA+TGG - Chr2:62653845-62653864 None:intergenic 35.0%
GAGAAGAGATTGAATCAAAG+TGG + Chr2:62653768-62653787 MsG0280010001.01.T01:CDS 35.0%
TCAAGCTGAAATCTTTCAAG+TGG + Chr2:62654370-62654389 MsG0280010001.01.T01:CDS 35.0%
TCCAAAGGATGAAACAAACA+TGG + Chr2:62653314-62653333 MsG0280010001.01.T01:CDS 35.0%
TCCATGTTTGTTTCATCCTT+TGG - Chr2:62653318-62653337 None:intergenic 35.0%
TGATGCTATTGAACACAATG+TGG + Chr2:62653947-62653966 MsG0280010001.01.T01:CDS 35.0%
TTCAGCATATGCACCTTTAA+CGG - Chr2:62653457-62653476 None:intergenic 35.0%
TTCCTAGCTAATTTCTTGCT+TGG - Chr2:62653234-62653253 None:intergenic 35.0%
! CAAGCTGAAATCTTTCAAGT+GGG + Chr2:62654371-62654390 MsG0280010001.01.T01:CDS 35.0%
! GAAATCTTTCAAGTGGGAAA+AGG + Chr2:62654377-62654396 MsG0280010001.01.T01:CDS 35.0%
! GAGACTTGTTCATCCAAAAA+TGG + Chr2:62654208-62654227 MsG0280010001.01.T01:CDS 35.0%
! GTTTTACCAATCCTAAAACC+AGG - Chr2:62654515-62654534 None:intergenic 35.0%
! TTTTGCTGAAATGGCTAAGA+GGG + Chr2:62654310-62654329 MsG0280010001.01.T01:CDS 35.0%
!! TGCTCTAAGATGAAGAACTT+TGG - Chr2:62653262-62653281 None:intergenic 35.0%
!! TTACTGTTGTTCCTGGTTTT+AGG + Chr2:62654501-62654520 MsG0280010001.01.T01:CDS 35.0%
!! TTTTTGGCAACATGATTCAC+AGG + Chr2:62654240-62654259 MsG0280010001.01.T01:CDS 35.0%
AATTGCGCAAACGATGATCT+TGG - Chr2:62654170-62654189 None:intergenic 40.0%
AGAAGTTGGAATCACAGGTT+AGG + Chr2:62653676-62653695 MsG0280010001.01.T01:CDS 40.0%
AGCTGAATCGAAGGAGATAA+AGG + Chr2:62653623-62653642 MsG0280010001.01.T01:CDS 40.0%
CACCAAGCAAGAAATTAGCT+AGG + Chr2:62653229-62653248 MsG0280010001.01.T01:CDS 40.0%
CAGAAGCTTCAATTCCATCA+GGG - Chr2:62653506-62653525 None:intergenic 40.0%
CATGCAGAAGAATCAACTCA+AGG + Chr2:62654559-62654578 MsG0280010001.01.T01:CDS 40.0%
GCTAGCATTTCAACCGTTAA+AGG + Chr2:62653441-62653460 MsG0280010001.01.T01:CDS 40.0%
GCTGAATCGAAGGAGATAAA+GGG + Chr2:62653624-62653643 MsG0280010001.01.T01:CDS 40.0%
TCAATTCCATCAGGGTCAAA+AGG - Chr2:62653498-62653517 None:intergenic 40.0%
TCAGAAGAAGAACCAGTTGT+TGG + Chr2:62654476-62654495 MsG0280010001.01.T01:CDS 40.0%
TCAGAAGCTTCAATTCCATC+AGG - Chr2:62653507-62653526 None:intergenic 40.0%
! ATTCACAGGAACCTTTTGAG+TGG + Chr2:62654254-62654273 MsG0280010001.01.T01:CDS 40.0%
! CTTTTGCTGAAATGGCTAAG+AGG + Chr2:62654309-62654328 MsG0280010001.01.T01:CDS 40.0%
!! GATCGATTCGAAACTTTGTG+AGG + Chr2:62653874-62653893 MsG0280010001.01.T01:CDS 40.0%
!! TGAGTTGATTCTTCTGCATG+AGG - Chr2:62654559-62654578 None:intergenic 40.0%
!!! GTTGGTTTTACTGTTGTTCC+TGG + Chr2:62654494-62654513 MsG0280010001.01.T01:CDS 40.0%
!!! GTTGTTCCTGGTTTTAGGAT+TGG + Chr2:62654506-62654525 MsG0280010001.01.T01:CDS 40.0%
AGTATCGCTTTCTCTGGTGA+AGG - Chr2:62653600-62653619 None:intergenic 45.0%
CTGTGATTCCAACTTCTTCG+CGG - Chr2:62653673-62653692 None:intergenic 45.0%
CTTCTTCGCGGTAATCTCAT+AGG - Chr2:62653661-62653680 None:intergenic 45.0%
GAAACTCTCCACCACTCAAA+AGG - Chr2:62654268-62654287 None:intergenic 45.0%
GATACTTGCAGCTGAATCGA+AGG + Chr2:62653614-62653633 MsG0280010001.01.T01:CDS 45.0%
TGAGATTACCGCGAAGAAGT+TGG + Chr2:62653662-62653681 MsG0280010001.01.T01:CDS 45.0%
!! TCATCAGGAAGTGCCTCATT+TGG - Chr2:62654065-62654084 None:intergenic 45.0%
!! TGCTACTGTTTTTCGTCACG+CGG + Chr2:62653848-62653867 MsG0280010001.01.T01:CDS 45.0%
CATCGTTTGCGCAATTCTGC+AGG + Chr2:62654174-62654193 MsG0280010001.01.T01:CDS 50.0%
CGCGAAGAAGTTGGAATCAC+AGG + Chr2:62653671-62653690 MsG0280010001.01.T01:CDS 50.0%
! CAGACACCTTTTGACCCTGA+TGG + Chr2:62653489-62653508 MsG0280010001.01.T01:CDS 50.0%
! GCTGCAAGTATCGCTTTCTC+TGG - Chr2:62653606-62653625 None:intergenic 50.0%
!! CACAGGAACCTTTTGAGTGG+TGG + Chr2:62654257-62654276 MsG0280010001.01.T01:CDS 50.0%
!! TTGCTTGGTGTCACTGCTGA+AGG - Chr2:62653219-62653238 None:intergenic 50.0%
AAAGGATCACGATCGCGTGC+TGG + Chr2:62654584-62654603 MsG0280010001.01.T01:CDS 55.0%
! GTGCTGGTCCGATTTCACAC+AGG + Chr2:62654600-62654619 MsG0280010001.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
Chr2 gene 62653201 62654628 62653201 ID=MsG0280010001.01;Name=MsG0280010001.01
Chr2 mRNA 62653201 62654628 62653201 ID=MsG0280010001.01.T01;Parent=MsG0280010001.01;Name=MsG0280010001.01.T01;_AED=0.38;_eAED=0.38;_QI=0|-1|0|1|-1|1|1|0|475
Chr2 exon 62653201 62654628 62653201 ID=MsG0280010001.01.T01:exon:34724;Parent=MsG0280010001.01.T01
Chr2 CDS 62653201 62654628 62653201 ID=MsG0280010001.01.T01:cds;Parent=MsG0280010001.01.T01
Gene Sequence

>MsG0280010001.01.T01

ATGGAATCAATGAAACCTTCAGCAGTGACACCAAGCAAGAAATTAGCTAGGAATTTCGCCAAAGTTCTTCATCTTAGAGCATTTTTGAAGAATGTTACTATATCTGATACAAATCCAAAGGATGAAACAAACATGGAAAAGACTACAATAACTTGGTCTGAATCATTCAAAAAAGTTGATGAAGATGAAGAGATTGAAGAACAAAGAGTTGCAACAGAAGCACTTCTTTCAAAGATTTTTGCTAGCATTTCAACCGTTAAAGGTGCATATGCTGAACTGCAACATTTTCAGACACCTTTTGACCCTGATGGAATTGAAGCTTCTGATAAACTACTAGTTTCTGAGTTGAAGCATTTATCTGAGCTAAAACAATGTTACTTGAAGAAACAATTTGATCCTTCACCAGAGAAAGCGATACTTGCAGCTGAATCGAAGGAGATAAAGGGTGTCATAAAAACCTATGAGATTACCGCGAAGAAGTTGGAATCACAGGTTAGGCTCAAAGATTCTGAGATTATGTTTCTTAAAGAGAAATTAGTGGAAGCTAATAGTCATAACAAGTTGATTGAGAAGAGATTGAATCAAAGTGGAACATTATCAGTACTTGATAATGTTGTTCACTTATCAGGGTTAAGTCCTAGCCATTTTGCTACTGTTTTTCGTCACGCGGTTAGATCGATTCGAAACTTTGTGAGGTTAATTGTTGATGAAATGAGATCTGCAAAATGGGATATTGATGCTGCTGTTGATGCTATTGAACACAATGTGGTTTATATGATAGAAGATCACAAGTGTTTCACAATTGAATCTTTTGTTTGCAAAGAAATGTTTGATGCATTTCACTTCCCTAATTTCAATCTTCCAAATGAGGCACTTCCTGATGATAGAAAAAATCAACAAAATTGGTTCTTTGAAAAGTTCAATGAGTTGAAATCAACGAAAGCTAAAGACTTTCTTGCTGAGAAACCAAGATCATCGTTTGCGCAATTCTGCAGGAACAAGTATTTGAGACTTGTTCATCCAAAAATGGAGTCATCATTTTTTGGCAACATGATTCACAGGAACCTTTTGAGTGGTGGAGAGTTTCCAAAAAGTGATTTTTTTGCATCTTTTGCTGAAATGGCTAAGAGGGTTTATCTCTTGCATTGTTTAGCCTTTTCTTTTGAGGTTCAAGCTGAAATCTTTCAAGTGGGAAAAGGGTGTAGATTTTCTGATGTGTACATGGAAAGTGTGAATGATGAAATGTTTGTGTTTTCAGACAAGACAGTTGTTGAATCAGAAGAAGAACCAGTTGTTGGTTTTACTGTTGTTCCTGGTTTTAGGATTGGTAAAACTGTTTTACAGTGTCAAATTTACCTCATGCAGAAGAATCAACTCAAGGTGAAAGGATCACGATCGCGTGCTGGTCCGATTTCACACAGGAGTTAA

Protein sequence

>MsG0280010001.01.T01

MESMKPSAVTPSKKLARNFAKVLHLRAFLKNVTISDTNPKDETNMEKTTITWSESFKKVDEDEEIEEQRVATEALLSKIFASISTVKGAYAELQHFQTPFDPDGIEASDKLLVSELKHLSELKQCYLKKQFDPSPEKAILAAESKEIKGVIKTYEITAKKLESQVRLKDSEIMFLKEKLVEANSHNKLIEKRLNQSGTLSVLDNVVHLSGLSPSHFATVFRHAVRSIRNFVRLIVDEMRSAKWDIDAAVDAIEHNVVYMIEDHKCFTIESFVCKEMFDAFHFPNFNLPNEALPDDRKNQQNWFFEKFNELKSTKAKDFLAEKPRSSFAQFCRNKYLRLVHPKMESSFFGNMIHRNLLSGGEFPKSDFFASFAEMAKRVYLLHCLAFSFEVQAEIFQVGKGCRFSDVYMESVNDEMFVFSDKTVVESEEEPVVGFTVVPGFRIGKTVLQCQIYLMQKNQLKVKGSRSRAGPISHRS*